Disclosure of Invention
In view of this, the invention provides a multi-channel microscopic observation counting frame with more reasonable structural design, improved detection efficiency and automatic detection prospect, and a clamping device with the counting frame.
The technical scheme of the invention is realized as follows: the invention provides a multichannel microscopic observation counting frame, which comprises a fixing frame and a multichannel cartridge, wherein the multichannel cartridge is of a flat plate structure, the fixing frame is arranged on the outer side of the multichannel cartridge in a surrounding manner along the circumferential direction of the multichannel cartridge, the inner side of the multichannel cartridge is provided with a plurality of parallel channels, two ends of the multichannel cartridge along the length direction of the parallel channels are embedded in the fixing frame and are mutually sealed with the surface of the fixing frame, the surface of the fixing frame is provided with a plurality of butt joint channels in a penetrating manner, two ends of each parallel channel are mutually communicated with the surface of the multichannel cartridge, and two ends of each parallel channel are mutually communicated with the surface of the fixing frame through a unique corresponding butt joint channel respectively, as a concrete matter, the multichannel cartridge adopts a transparent material, the parallel channel structure is used for replacing a closed surrounding counting structure in a conventional counting frame, and a pipeline feeding manner can be adopted, replace conventional manual operation, for the automated inspection provides probably, also improved the efficiency of detection, parallel passage's both ends opening is through butt joint passageway and count frame outside intercommunication, utilizes connection structure direct and count frame surface to communicate the opening that meets the passageway, realizes the normal use of count frame.
On the basis of the technical scheme, preferably, the multichannel card casket includes roof, runner plate and bottom plate that the preface parallel arrangement in proper order, a plurality of parallel grid holes have been seted up on the surface of runner plate, and roof and bottom plate centre gripping grid hole form parallel channel, the position that the surface of roof or bottom plate is close to grid hole both ends has seted up the through-hole, and when the multichannel card casket embedding was installed in fixed frame, every through-hole communicates with an only butt joint passageway that corresponds each other respectively.
On the basis of the technical scheme, preferably, the top plate and the bottom plate are made of transparent materials.
On the basis of above technical scheme, it is preferred, fixed frame still includes fixed plate and two grip blocks, and the centre of fixed plate is link up and is equipped with the opening, and one of them side surface of fixed plate is seted up flutedly, and the opening is seted up in the recess, and multichannel card casket embedding is installed in the recess, the grip block tight mounting is at the fixed plate along the both ends of recess length direction, and two grip blocks compress tightly the both ends of multichannel card casket on the surface of fixed plate respectively.
It is further preferred that the docking channels open onto the clamping plate, the docking channels communicating with the parallel channels of the multi-channel cartridge when the clamping plate is securely mounted on the holding plate.
On the basis of the technical scheme, the improved fixing frame is preferred and further comprises a sealing ring, a sealing groove is formed in the surface of the fixing frame and is located at the opening where the butt joint channel is communicated with the parallel channel along the circumferential direction, and the sealing ring is embedded into the sealing groove and is installed.
On the basis of the technical scheme, preferably, the two ends of the fixing frame in the width direction of the parallel channel are provided with wedge-shaped surfaces.
The invention also provides a clamping device comprising the multichannel microscopic observation counting frame, and the clamping device further comprises a clamping plate and a buckle, wherein the surface of the clamping plate is provided with a mounting groove, the mounting groove comprises a first side surface, the first side surface and the bottom surface of the mounting groove form an acute angle, one side of the mounting groove, which is opposite to the first side surface, is provided with the buckle in a sliding manner, the sliding direction of the buckle is vertical to the length direction of the first side surface, one side of the buckle, which is opposite to the first side surface, is provided with a second side surface, the second side surface and the bottom surface of the mounting groove form an acute angle, and when the fixing frame is embedded in the mounting groove, the buckle slides, so that the first side surface and the second side surface are selectively mutually propped against two wedge-shaped surfaces of the fixing frame and tightly press the fixing frame in the mounting groove.
On the basis of the technical scheme, preferably, still include resilient means, resilient means installs and keeps away from between the one end of first side and the clamping board at the buckle, thereby resilient means drive buckle towards the direction motion of being close to first side makes first side and second side push up tightly each other with two wedge faces of fixed frame respectively.
On above technical scheme's basis, it is preferred, still include the slider, the spout has all been seted up at the mounting groove along first side length direction's both ends, and slider slidable mounting is in the spout, a plurality of connect the via hole have been seted up on the surface of slider, when fixed frame embedding in the mounting groove and first side and second side and two wedge faces of fixed frame each other when pushing up tightly, the slider slides and selectively pushes up the surface of tight fixed frame, when the surface of the tight fixed frame in slider top, parallel passage's one end is all just to setting up with a connect the via hole to every butt joint passageway is kept away from.
On above technical scheme's basis, preferred, still include communicating pipe, wherein one end of communicating pipe is fixed to be set up in connect the via hole, and when the surface of the tight fixed frame in slider top, the tip embedding butt joint passageway of communicating pipe is interior.
Compared with the prior art, the multi-channel microscopic observation counting frame and the clamping device with the counting frame have the following beneficial effects:
(1) the counting frame adopts the channel structure as a container for temporary storage and observation of microorganisms, replaces a conventional manually filled closed counting frame, has the advantages of easy manufacture of samples and easy realization of automation, and simultaneously has a plurality of parallel channels, so that the parallel observation of a plurality of samples can be realized simultaneously, and the utilization rate of the counting frame and the efficiency of sample detection are improved;
(2) the channel type structure is convenient to be matched with a fluid conveying and valve structure, so that automatic sample feeding, sample discharging, cleaning, blow-drying and other operations are realized, the detection efficiency is further improved, and the workload of manual operation is greatly reduced;
(3) the clamping device is matched with the counting frame structure, so that the counting frame can be quickly mounted and dismounted, the counting frame can be positioned and fixed by the clamping device, automatic detection processing is convenient to realize, the clamping device is provided with the buckle structure, and the buckle and the wedge-shaped surface are matched with the counting frame body to realize quick clamping and dismounting;
(4) the clamping device is provided with the sliding slide block, the slide block is provided with the connecting through hole, the channel opening on the counting frame can be conveniently butted, and the connecting through hole can be internally provided with the connecting pipe structure, so that the slide block is utilized to realize quick butt joint and communication of a plurality of openings, and the efficiency of butt joint installation of pipelines is improved.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, it is obvious that the drawings in the following description are only some embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to the drawings without creative efforts.
FIG. 1 is an isometric view of a clamping device having a multi-channel microscopic observation counting frame according to the present invention;
FIG. 2 is a schematic diagram of the exploded structure of FIG. 1;
FIG. 3 is a schematic view of the chuck portion in an exploded condition in accordance with the present invention;
FIG. 4 is a schematic view of another perspective of the clamping plate of the present invention showing a partially exploded view;
FIG. 5 is an isometric view of a multi-channel cassette of the present invention in a perspective state;
FIG. 6 is an exploded view of a multi-channel cassette of the present invention;
FIG. 7 is an isometric view of a multi-channel cassette of another embodiment of the invention;
FIG. 8 is an exploded view of a multi-channel cassette according to another embodiment of the invention;
FIG. 9 is an isometric view of a multi-channel cassette of another embodiment of the invention;
FIG. 10 is an exploded view of FIG. 9;
fig. 11 is a perspective view of fig. 10.
In the figure: 1-fixing frame, 2-multichannel cassette, 3-clamping plate, 4-buckle, 5-elastic device, 6-slide block, 7-communicating pipe, 8-sealing ring, 11-fixing plate, 12-clamping plate, 13-sealing groove, 14-wedge surface, 21-top plate, 22-runner plate, 23-bottom plate, 221-grid hole, 201-parallel channel, 202-through hole, 31-mounting groove, 32-first side surface, 33-sliding groove, 41-second side surface and 61-connecting through hole.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be obtained by a person skilled in the art without any inventive step based on the embodiments of the present invention, are within the scope of the present invention.
As shown in fig. 1, with reference to fig. 2 to 11, the multichannel microscopic observation counting frame of the present invention includes a fixing frame 1 and a multichannel cartridge 2, wherein the multichannel cartridge 2 is a flat plate structure, the fixing frame 1 is installed outside the multichannel cartridge 2 along the circumferential direction of the multichannel cartridge 2, the multichannel cartridge 2 is provided with a plurality of parallel channels 201 on the inner side, both ends of the multichannel cartridge 2 along the length direction of the parallel channels 201 are embedded in the fixing frame 1 and are mutually sealed with the surface of the fixing frame 1, the surface of the fixing frame 1 is provided with a plurality of docking channels 101 in a penetrating manner, both ends of each parallel channel 201 are mutually communicated with the surface of the multichannel cartridge 2, and both ends of each parallel channel 201 are mutually communicated with the surface of the fixing frame 1 through a unique corresponding docking channel 101.
In the above embodiment, the multi-channel cassette 2 is provided with a plurality of parallel channels 201, the parallel channels 201 are communicated with the docking channel 101, the docking channel 101 can be used to directly inject the water sample to be tested into the parallel channels 201, and the water sample forms the observation sample in the multi-channel cassette 2, it should be understood that, in order to facilitate the observation of the multi-channel cassette 2, it should be made of transparent material, and particularly, in order to maintain the stability of the sample liquid during observation, the opening and closing structure of the pipeline can be arranged on the peripheral input and output pipeline, the environment in the parallel channel 201 is sealed or not sealed by controlling the opening and closing of the pipeline, thereby achieving the purpose of selectively stabilizing the water sample to be tested, due to the pipeline structure formed by the parallel channels 201, the external input and output structure with controllable fluid can be directly communicated with the fixed frame 1, so that the automatic sample feeding and discharging processing is realized.
This application adopts parallel channel 201 as the container of observing the count, still conveniently removes and washs the operation to the sample after the detection.
In the specific embodiment, the multi-channel cassette 2 comprises a top plate 21, a flow channel plate 22 and a bottom plate 23 which are sequentially arranged in parallel, wherein a plurality of parallel grid holes 221 are formed in the surface of the flow channel plate 22, the grid holes 221 are clamped by the top plate 21 and the bottom plate 23 to form parallel channels 201, through holes 202 are formed in the positions, close to two ends of the grid holes 221, of the surface of the top plate 21 or the bottom plate 23, and when the multi-channel cassette 2 is embedded in the fixing frame 1, each through hole 202 is communicated with one unique corresponding butt joint channel 101.
The multichannel cassette 2 formed by connecting the three layers of structures enables the preparation difficulty to be greatly reduced, as a specific implementation mode, the top plate 21, the runner plate 22 and the bottom plate 23 are fixedly connected, and the specific connection mode is preferably that adhesive is used for sealing and bonding the three layers of structures, and the top plate 21 or the bottom plate 23 is provided with a through hole 202 for providing an inlet and outlet opening for the parallel channel 201.
The open sides of the grid holes 221 are sealed to the top plate 21 and the bottom plate 23 to form a thin-layer chamber structure, i.e., the parallel channels 201.
In a specific embodiment, the fixing frame 1 further includes a fixing plate 11 and two clamping plates 12, an opening 111 is formed through the middle of the fixing plate 11, a groove 112 is formed in one side surface of the fixing plate 11, the opening 111 is formed in the groove 112, the multichannel cartridge 2 is embedded in the groove 112, the clamping plates 12 are tightly mounted at two end portions of the fixing plate 11 along the length direction of the groove 112, and the two clamping plates 12 respectively press the two end portions of the multichannel cartridge 2 against the surface of the fixing plate 11.
Fixed frame 1 is used for fixing multichannel card casket 2 and makes multichannel card casket 2 can be directly connected with outside intercommunication pipeline as intermediate junction structure, opening 111 is as the window of observing multichannel card casket 2, also can regard as operation window convenience to carry out specific operation to multichannel card casket 2 simultaneously, for example remove etc., fixed plate 11 and grip block 12 mutually support, it is fixed to carry out the centre gripping to multichannel card casket 2, it is concrete, multichannel card casket 2 imbeds in recess 112, two grip blocks 12 carry out the centre gripping respectively to the both ends that multichannel card casket 2 is relative fixed.
In the embodiment, the docking channel 101 is opened on the clamping plate 12, and when the clamping plate 12 is tightly mounted on the fixing plate 11, the docking channel 101 and the parallel channel 201 of the multi-channel cassette 2 are communicated with each other.
In the above embodiment, the clamping plate 12 not only clamps and fixes the multi-channel cartridge 2, but also the docking channel 101 is disposed on the clamping plate 12, and when the clamping plate 12 clamps the multi-channel cartridge 2, the docking channel 101 is docked with the through hole 202 on the multi-channel cartridge 2 to communicate with the through hole, and by using the above connection method, on one hand, the multi-channel cartridge 2 can be disassembled and replaced, and the clamping plate 12 can be disassembled and replaced, so that the number of the parallel channels 201 can be adjusted, and at the same time, because the multi-channel cartridge 2 is used for detecting planktonic microorganisms in the water body, dirt exists in the multi-channel cartridge 2 after long-time use, and the conventional washing may not reach good washing requirements, therefore, the detachable structure can facilitate the cleaning of the multi-channel cartridge 2, and at the same time, by disposing the docking channel 101 on the detachable clamping plate 12, the clamping plate 12 can be conveniently disassembled and cleaned.
In a specific embodiment, the fixing frame further comprises a sealing ring 8, a sealing groove 13 is formed in the surface of the fixing frame 1 at the opening where the butt joint channel 101 and the parallel channel 201 are communicated along the circumferential direction, and the sealing ring 8 is embedded in the sealing groove 13.
In the above embodiment. The fixing frame 1 and the multi-channel cassette 2 are connected in a contact mode, and therefore the connection position between the butt joint channel 101 and the parallel channel 201 may have the problem of poor fit.
In a particular embodiment, the fixing frame 1 is provided with wedge surfaces 14 at both ends in the direction parallel to the width of the channel.
The wedge-shaped surface 14, as a fixing force applying point structure of the fixing frame 1, can be mutually matched with an external wedge-shaped structure, and realizes the compression fixation of the fixing frame 1 by utilizing the inclined surface effect.
In a specific embodiment, the invention further provides a clamping device with a multi-channel microscopic observation counting frame, which further comprises a clamping plate 3 and a buckle 4, wherein a mounting groove 31 is formed in the surface of the clamping plate 3, the mounting groove 31 comprises a first side surface 32, the first side surface 32 forms an acute angle with the bottom surface of the mounting groove 31, the buckle 4 is slidably mounted on one side of the mounting groove 31, which is opposite to the first side surface 32, the sliding direction of the buckle 4 is perpendicular to the length direction of the first side surface 32, a second side surface 41 is arranged on one side of the buckle 4, which is opposite to the first side surface 32, the second side surface 41 forms an acute angle with the bottom surface of the mounting groove 31, and when the fixing frame 1 is embedded in the mounting groove 31, the buckle 4 slides, so that the first side surface 32 and the second side surface 41 selectively abut against the two wedge surfaces 14 of the fixing frame 1 and press the fixing frame 1 in the mounting groove 31.
In the above embodiments, in order to achieve the better automatic detection function of the counting frame of the present application, the installation and fixing operations in the using process of the counting frame need to be simplified, and the conventional counting frame structure is generally fixed on the objective table of a microscope in a manual manner for counting, the fixing frame 1 of the present application has a channel-type counting cavity, so that the rapid active sample introduction and sample discharge of a sample can be performed, and relatively, the fixing frame 1 does not need to be moved by multiple manual operations, so the present application provides a clamping device, which fixes the clamping device, specifically, the clamp 4 slides on the clamping plate 3, the clamp 4 and the clamping plate 3 are respectively provided with the second side surface 41 and the first side surface 32, the two side surfaces respectively correspond to the wedge surfaces 14 on the two sides of the fixing frame 1, and because the two side surfaces both form an acute angle with the bottom surface of the installation groove 31, therefore, when the fixing frame 1 is embedded in the mounting groove 31, the first side surface 32 and the second side surface 41 can be respectively attached to the surfaces of the two wedge surfaces 14 by moving the buckle 4 to tightly push the fixing frame 1, so that a wedge-shaped compression structure is formed, and the fixing frame 1 is compressed in the mounting groove 31.
The cell body can be seted up on the surface of clamping plate 3, and buckle 4 slides and sets up in the cell body, and as further, in order to realize compressing tightly fixed frame 1, the reaction force can order about buckle 4 and break away from the cell body, consequently can set up limit structure in the cell body, for example set up the gag lever post parallel with 4 slip directions of buckle in the cell body, the gag lever post runs through buckle 4, and limit structure is used for preventing buckle 4 to break away from the cell body.
In the specific embodiment, the elastic device 5 is further included, the elastic device 5 is installed between one end of the buckle 4 far away from the first side surface 43 and the clamping plate 4, and the elastic device 5 drives the buckle 4 to move towards the direction close to the first side surface 32 so as to enable the first side surface 32 and the second side surface 41 to be respectively and mutually abutted against the two wedge-shaped surfaces 14 of the fixing frame 1.
In the above embodiment, the buckle 4 is driven to tightly abut against the fixed frame 1 in a sliding manner, and the corresponding fixed frame 1 also generates a reaction force to drive the buckle 4 to separate from the fixed frame 1, so that in order to satisfy the stability of the buckle 4 abutting against the fixed frame 1, the elastic device 5 is further provided for driving the buckle 4 to tightly abut against the fixed frame 1, and specifically, the elastic device 5 may be a spring or other structures having stable elastic driving force.
Similarly, in order to separate the fixing frame 1 from the mounting groove 31, the engaging piece 4 needs to be driven to be away from the fixing frame 1, so that the fixing frame 1 can be separated from the wedge-shaped pressing structure, and in order to apply force to the engaging piece 4, it is preferable that a groove for applying force is formed on the surface of the engaging piece 4.
In the concrete implementation mode, still include slider 6, spout 33 has all been seted up at the both ends of first side 32 length direction along mounting groove 31, and slider 6 slidable mounting is in spout 33, a plurality of connect the via hole 61 have been seted up on the surface of slider 6, when fixed frame 1 embedding in mounting groove 31 and first side 32 and second side 41 and fixed frame 1 two wedge surfaces 14 when tightly jacking each other, slider 6 slides and selectively tightly jacks up the surface of fixed frame 1, and when slider 6 tightly jacks up the surface of fixed frame 1, the one end that parallel passage 201 was kept away from to every butt joint passageway 101 all just sets up with a connect the via hole 61.
In the above embodiment, be equipped with in fixed frame 1 and dock passageway 101 and be used for communicateing with parallel channel 201, consequently, be equipped with the opening of docking passageway 101 on the surface of fixed frame 1, in order to facilitate the pipeline with the outside and dock the opening of docking passageway 101, this application adopts slider 6 as guide structure, set up connect the through-hole 61 on the surface of slider 6, slider 6 slides to when butting with fixed frame 1, connect the through-hole 61 just to the opening of docking passageway 101, thereby make things convenient for operating personnel to utilize connect the through-hole 61 to insert the pipeline in docking passageway 101, through this design, can let equipment realize the connection of pipeline more fast and automizedly, connect the through-hole 61 can regard as an alignment structure, slider 6 then is as the integrated connector of connecting tube, can drive the connection and the disconnection of pipeline promptly through drive slider 6.
In a preferred embodiment, the sliding direction of the sliding block 6 is parallel to the axial direction of the connecting through hole 61, so that the pipe can be directly and fixedly arranged in the connecting channel 61, and the sliding block 6 is driven to move, that is, the communication between the external pipe and the docking channel 101 can be realized.
As a further preferable embodiment, in order to satisfy the close contact at the joint between the connection channel 61 and the docking channel 101, a sealing structure may be provided at an opening of the connection channel 61 near one end of the fixed frame 1, or at an opening of the docking channel 101 near one end of the slider 6.
In the specific embodiment, the docking device further comprises a communication pipe 7, wherein one end of the communication pipe 7 is fixedly arranged in the connection through hole 61, and when the slider 6 abuts against the surface of the fixed frame 1, the end of the communication pipe 7 is embedded in the docking channel 101.
In the above embodiment, the communication pipe 7 is directly disposed in the connection through hole 61, and the slider 6 is moved to communicate and dock the communication pipe 7 with the docking passage 101, as a preferable feasible embodiment of the above embodiment, the axial direction of the connection through hole 61 is parallel to the moving direction of the slider 6.
As a further preference, the docking channel 101 is provided with a sealing ring in the circumferential direction close to the inside of the opening of the slider 6.
The above description is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the invention, and any modifications, equivalents, improvements and the like that fall within the spirit and principle of the present invention are intended to be included therein.