CN1139092A - Concentrated strain powder for microbial fertilizer and its production - Google Patents
Concentrated strain powder for microbial fertilizer and its production Download PDFInfo
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- CN1139092A CN1139092A CN 96104890 CN96104890A CN1139092A CN 1139092 A CN1139092 A CN 1139092A CN 96104890 CN96104890 CN 96104890 CN 96104890 A CN96104890 A CN 96104890A CN 1139092 A CN1139092 A CN 1139092A
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- gemma
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- phosphorus
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Abstract
Nitrogen-fixing bacteria, phosphobacteria and potassium bacteria producing gemma are made into concentrateds gemma powder through the process of culturing fermentation, dewatering in plate-frame filter and drying in hot blast of the temp lower than 80 deg.C. The strain powder thus produced has high gemma density (10 billion/gram) and is easy to store and transport. The said method can produce strain centrally for various mcirobial fertilizer plants.
Description
The present invention relates to a kind of novel process of novel microorganism manure strain agent manufacture method.More particularly, the present invention relates to novel process and the novel method that a kind of novel microorganism manure strain agent that will contain the fixed nitrogen sporeformer is made the gemma powder of concentrate drying.
In last patent of invention " solid-nitrogen bud-fungus fertilizer and manufacture method thereof " (number of patent application 951174320) application form, adopt following a kind of novel microorganism fertilizer and manufacture method thereof that contains the fixed nitrogen sporeformer: (a) select domestic distinctive fixed nitrogen gemma bacterial classification (Bacillus azotofixans) and the known sporiferous phosphorus bacteria and the potassium bacteria culture that can produce gemma of newly isolating for use, bulk fermentation is cultivated and is formed gemma in fermentor tank; (b) three kinds of zymocyte liquids are mixed by a certain percentage with organism matrix such as domestic sludge that passes through germicidal treatment and manure of livestock and poultry, in mechanical means, manufacture the spheroidal particle; (c) after 60～80 ℃ of oven dry, make the microorganism organic composite fertilizer material that contains a large amount of vinelandii, phosphorus bacteria and potassium bacterial spore.The advantage of this method is to produce vinelandii, phosphorus bacteria and the potassium bacteria particles fertilizer that contains gemma, can high temperature resistant drying and storage.But weak point is all strain fermentating liquids only should be self-produced personal in the our factory, big through the strain liquid volume of fermentation culture, is difficult to packed and transported and becomes particle bacterial manure to other bacterial manure factory with local fertilizer raw material hybrid process; And various places township and village enterprises will produce this kind bacterial manure, certainly will all will set up strain plant fermentative production bacterial classification voluntarily.So, the facility investment and the technical force of the various places similar bio-feritlizer factory that then need be multiplied, and under township and village enterprises's insufficient funds and fermentation technique personnel shortage situation certainly will have influence on the development of composite bio-fertilizer and apply as no bacterial classification source.
In view of there is weak point in above-mentioned prior art, the present invention further studies and improves the bacterial classification manufacture method, the strain fermentating liquid concentrate drying is become the dry strain powder, and its purpose is to provide a kind of most economical effective fixed nitrogen sporeformer, phosphorus bacteria and the processing of potassium bacteria culture fermented liquid can being concentrated to make the making method that contains high density (every gram contains 10,000,000,000 of gemma) gemma strain powder.But it is standby to concentrate the gemma strain powder long storage of being processed into: (1) expands the bacterial classification of making particle bacterial manure for our factory's anniversary from being used as; (2) expand the usefulness of making particle bacterial manure through packed and transported to the processing of bio-feritlizer factory of various places township and village enterprises, the similar enterprise in various places does not need to invest in addition strain plant; (3) be used as the bacterial manure seed dressing, every mu only with 20 gram dry bacterial powders, contains 2,000 hundred million of effective bacterium, and 0.5～1 kilogram of every mu of consumption of peat composed of rotten mosses bacterial manure seed dressing of applying than existing various places (contain bacterium 500～1,000 hundred million) consumption lacks and effectively 1～4 times of bacterium amount increase.
Through test manufacture relatively, warm air drying became powder method concentrated strain gemma after the present invention adopted Plate Filtration, more save the energy and cost and rate of recovery height than other concentration method (as spraying drying or supercentrifugal process), thereby be that a kind of most economical effective microorganism manure strain powder is made new technology.
Purpose of the present invention can realize by following technical measures:
One, strain fermentation culture technique
(1) bacterial classification
1. bacillus azotobacter: adopt the patent bacterial classification CN9506 in the preceding paragraph application for a patent for invention book (951174320).Bacillus azotobacter CN9506 specifies depositary institution-Chinese typical culture collection center (CCTCC) preservation August 4 nineteen ninety-five in Patent Office of the People's Republic of China, is numbered CCTCC M95040.After this this bacterial strain is called bacillus azotobacter CCTCC M95040, for ease of narration, this paper abbreviates this bacterial strain as " vinelandii ".
The used vinelandii characteristics of the present invention are nitrogenase activity height (1100-3200 nM ethene/milligram albumen/hour), can produce statoblast, can tolerate 80 ℃ of high temperature dryings; Make behind the gemma powder sealing and preserve and be difficult for pollution microbes and go bad, but long storage is standby.
2. phosphorus bacteria: described phosphorus bacteria is meant bacillus megaterium (Bacilllusmegatherium) ACCC10010, because of it can decompose the soil insoluble phosphorus, therefore is commonly called as and is phosphorus bacteria.Phosphorus bacteria in China Committee for Culture Collection of Microorganisms's agricultural microorganism center (ACCC) preservation, is numbered ACCC10010, and the public can buy phosphorus bacteria to this preservation center by preserving number.
3. potassium bacterium: described potassium bacterium is meant that this bacterium of bacillusmusilaginosiengineering (Bacilllus mu-cilaginosus) ACCC10013 has the plain ability of potassium in the soil mineral of decomposition, therefore is commonly called as the potassium bacterium.The potassium bacterium in the center preservation of agriculture microorganism, is numbered AC-CC10013, and the public can be by number asking for.
(2) cultivation and fermentation condition: the cultivation and fermentation condition that comprises above-mentioned vinelandii, phosphorus bacteria and potassium bacteria culture.
1. the cultivation and fermentation of vinelandii can be used known culture condition and method.General incubation time is 20～24 hours, and culture temperature is 25～30 ℃, and cultivating pH is 7.0～7.4, air flow 0.5～1:1 cubic meter/1000 liters, 200～300 rev/mins of stirring velocitys.The preferred incubation time of this bacterial classification is 30 hours formation gemma, and 32 ℃ of culture temperature are cultivated pH7.7, air flow 0.5-1:1 cubic meter/1000 liters, tank pressure 0.5kg/cm
2, 200 rev/mins of stirring velocitys.Its preferred culture medium is as follows:
Screening and preservation bacterial classification nitrogen-free agar:
Sucrose 1.00%
Yeast extract paste 0.10%
Sal epsom (MgSO
4) 0.05%
Potassium primary phosphate (KH
2PO
4) 0.02%
Dipotassium hydrogen phosphate (K
2HPO
4) 0.008%
Sodium-chlor (NaCl) 0.001%
Lime carbonate (CaCO
3) 0.50%
Sodium orthomolybdate (Na
2MoO
4) 0.0005%
Ferrous sulfate (FeSO
4) 0.00015%
Agar 2.0%
pH＝7.7
Being used for the shake-flask culture of CCTCC M95040 vinelandii and the substratum of fermentative production is to add following composition on the basis of above-mentioned nitrogen-free agar in addition:
Semen Maydis powder 1%
Peptone 0.1%
Ammonium sulfate 0.1%
2. the cultivation of phosphorus bacteria and fermentation condition are undertaken by the method in published " phosphorus bacteria " volumes such as (, agriculture press, nineteen fifty-nine, Beijing) Chen Tingwei book.
The substratum of a large amount of cultivation and fermentation of phosphorus bacteria is: starch 3%, soybean cake powder 1%, peptone 0.1%, ammonium sulfate 0.2%, dipotassium hydrogen phosphate 0.2%, yeast powder 0.1%, lime carbonate 0.01%, pH=7.2-7.4.All the other fermentation conditions are identical with the fixed nitrogen sporeformer.
3. potassium microbial culture and fermentation condition are undertaken by the method in published " potassium bacterium " (Chen Tingwei compiles, agriculture press, nineteen fifty-nine, Beijing) book.
The substratum of a large amount of cultivation and fermentation of potassium bacterium is: starch 10%, soybean cake powder 10%, dipotassium hydrogen phosphate 0.2%, ammonium sulfate 0.1%, sal epsom 0.05%, iron trichloride 0.001%, lime carbonate 0.01%, ferment powder 0.01%, pH=7.2-7.4.All the other fermentation conditions are identical with fixed nitrogen sporeformer phosphorus bacteria.
Two, gemma bacterial classification concentrate drying becomes the powder technology
1. fermented liquid adsorption treatment
Vinelandii, phosphorus bacteria and the fermentation of potassium microbial culture 25～30 hours, through the gemma rate of formation of three kinds of thalline of microscopy, when the gemma rate reaches 80% or more and has 20% gemma can stop to ferment in deviating from from sporangiocyst.Then the fermented liquid of three kinds of bacterium is put into storage pool and mix, add about 20% light calcium carbonate carrier, stir that thalline fully is adsorbed on the carrier after 30 minutes as the absorption thalline.
The sorbent quantity that joins in the fermented liquid is calculated according to following formula:
Sorbent material add-on (kilogram)=
2. filter press concentrates:
Will through the fermented liquid of adsorption treatment with 0.2MPa (2 kg/cm) about, the pressure of the highest 0.6MPa, by being pressed into plate-and-frame filter press in the hold tank, filter by No. 7 filter clothes, the carrier (lime carbonate) that is adsorbed with thalline is stayed in the sheet frame, can be dewatered to become and contain the filter cake that concentrates the gemma thalline.When filter finishing, feed 0.2MPa pressurized air again and dry up filter cake and removed moisture in the filter cake in 15 minutes as far as possible, descend sheet frame to get filter cake then.With the filter cake morsel, be placed on the wire netting framework and deliver to baking room.
3. warm air drying
Adopt tunnel like drying room warm air drying equipment.With steam radiator element or pipeline is thermal source, through gas blower heated dry air is blown in the tunnel like drying room, makes to be loaded with thalline filter cake fragment on the wire netting framework and in 60 ℃～80C hot-air flow the moisture rapid evaporation to be dried.Tunnel entrance hot blast temperature≤80 ℃, temperature out 〉=50 ℃ can make the filter cake piece dry and be lower than 5% dried piece to water content through 3～4 hours.
4. crushing packing
The dried filter cake piece that contains the gemma thalline after the oven dry is smashed in pulverizer, made behind the dried sporeformer powder can package storage standby by 80 mesh sieves.
Three, the gemma dry bacterial powder contains the detection of bacterium number
According to the living spores bacterium number in the dilution plate counting process detection dry bacterial powder of routine.Take by weighing dry bacterial powder 1.00 grams, join and be diluted to proper concn (every milli contains several 20～200 of bacterium approximately) in 99 ml sterile waters, pipetting 1 milliliter of dilution bacterium liquid then joins in the aseptic cultivation first ware, add vinelandii or phosphorus bacteria or potassium bacteria culture medium after dissolving in right amount respectively, form dull and stereotyped after the thorough mixing condensation, in 30 ℃ of insulation cans, cultivated two days, calculate the colony number on the culture dish flat board.The counting of every kind of bacterium is dull and stereotyped to be repeated 3 times, gets the average colony number that grows on the counting flat board, multiply by the viable count that the diluted sample multiple is to be had in every gram dry bacterial powder (being the living spores number), and its Counting Formula is as follows:
The dry bacterial powder final product quality requires:
3 kinds of bacterium living spores are counted total content: 〉=100 * 10
8(hundred million /) (restraining wherein, vinelandii, phosphorus bacteria, potassium bacterium respectively account for 1/3)
Dry bacterial powder water content:＜5%
Dry bacterial powder fineness: cross 80 mesh sieve holes more than 95%
Below in conjunction with embodiment the present invention is described:
Embodiment 1 (vinelandii gemma powder)
1. fixed nitrogen bacterial classification cultivation and fermentation
Bacillus azotobacter CCTCC M95040 is inoculated into the triangular flask shake-flask culture 16 hours that fill 1000ml foregoing is used for vinelandii substratum of the present invention, be inoculated in 200 liters of seed fermentation jars by 1% inoculum size then, ventilation (1: 0.5), stirred 30 ℃ of heat insulating culture 16 hours, be inoculated in 2000 liters of big fermentor tanks by 10% inoculum size again, similarity condition cultivate be warmed up to after 20 hours 35 ℃ and strengthen air flow (1: 1) cultivate again treated in 10 hours thalline gemma rate of formation reach 80% and have 20% gemma from sporangiocyst, to deviate from after can go out jar and become and produce gemma fixed nitrogen strain fermentating liquid.
2. the preparation of gemma bacterial classification concentrated dry powder
The vinelandii fermented liquid that forms gemma is put into hold tank, add 20% light calcium carbonate, stir that the thalline gemma fully is adsorbed on the lime carbonate after 30 minutes, then with 0.2MPa pressure by being pressed into plate-and-frame filter press in the basin, can elimination moisture through about 1 hour and the lime carbonate that will adsorb the gemma thalline is stayed to become in the sheet frame filter cloth and contained bacterium filter cake piece.Then the filter cake piece is broken into fritter, delivers to baking room and be lower than 5% dried piece through 60～80 ℃ of warm air drying to water content, can package storage standby through crushing screening again.The bacterial classification dry powder that is prepared into by this method should contain several 10,000,000,000 of gemma/gram, and dry storage active maintenance in 2 years is more than 80%.
The strain powder of making by the present invention can become particle bacterial manure or uses as the seed dressing of farm crop with strain powder for our factory or other factory with fertilizer matrix hybrid process.
3. be a granulated into particle bacterial manure with fertilizer matrix hybrid process
Municipal sludge 60kg and chicken manure 30kg were pulverized 80 mesh sieves,, add 10kg ammonium sulfate then and be mixed into fertilizer matrix through 200 ℃ of hot blast cylinder oven dry killing pathogenic bacteria and worm's ovum.Add 0.5～1% vinelandii gemma powder thorough mixing made of step as described above in above-mentioned fertilizer matrix, spray adds 20% moisture and rolls and make diameter 3～5mm spheroidal particle in pan-pelletizer, promptly makes the particulate state azotobacteria fertilizer through 80 ℃ of hot blast roller dryings again.This product contains fixed nitrogen sporeformer 0.5～100,000,000/gram, and dry storage active maintenance in 2 years is more than 80%.
Embodiment 2 (phosphorus bacteria gemma powder)
Cultivate the phosphorus bacteria fermented liquid by aforementioned phosphorus bacteria substratum and embodiment 1 described method, again through making the phosphorus bacteria gemma powder that concentrates processing with method Plate Filtration and oven dry.10,000,000,000/gram of the phosphorous bacterial spore of the phosphorus bacteria strain powder of making, can make bacterial classification for our factory or nonlocal bacterial manure factory, as described in embodiment 1, join fertilizer matrix in 0.5～1% ratio and promptly can be made into granular phosphorus bacterium fertilizer, every gram particle bacterial manure contains phosphorus bacteria 0.5～100,000,000.
Embodiment 3 (potassium bacterial spore powder)
Cultivate the potassium ferment product by method described in aforementioned potassium bacteria culture medium and the embodiment 1, again through make the potassium bacteria culture powder of concentrate drying with method Plate Filtration and oven dry.Potassium bacterium dry powder after concentrating contains 10,000,000,000 of potassium bacterial spores/gram, can supply our factory or nonlocal factory as the bacterial classification of making bacterial manure.Method described in embodiment 1-3 joins mixing granulation in the fertilizer matrix in 0.5～1% ratio, promptly can be made into particle potassium bacterial fertilizer, and every gram finished product contains potassium bacterium 0.5～100,000,000.
Embodiment 4 (composite bacteria gemma powder)
Press method described in embodiment 1, example 2 and the example 3 fermentation culture bacillus azotobacter CCTCC M95040 and phosphorus bacteria and potassium bacteria culture respectively, form and to put into hold tank behind the gemma and add 20% lime carbonate again, stir that lime carbonate fully adsorbs the gemma thalline after 30 minutes.Then mixed bacteria liquid and sorbent material are pressed into flame filter press, dehydration is condensed into filter cake, and drying, crushing screening are made and contained vinelandii, phosphorus bacteria and potassium bacterium three bacterium blended sporeformer powder again.Three kinds of bacterium compound gemma powder contain 10,000,000,000 of gemma/gram.
As described in embodiment 1～example 3, in fertilizer matrix, add 0.5～1% composite bacteria gemma powder and promptly can be made into the organic-microbial compound bacterial fertilizer granulated fertilizer that contains vinelandii, phosphorus bacteria and potassium bacterium.This kind product contains three kinds of composite bacteria 0.5～100,000,000/gram, meets that the particulate state composite microbiological fertilizer contains effective bacterium requirement in the speck fertilizer industry standard (NY227-94) that present China Ministry of Agriculture issues.
The composite microbiological fertilizer that the present invention makes in 23 counties (city) experimental tests in 21 provinces (city, district) in 1994, has 85.7% (referring to No. 05 literary composition of national agricultural technology extension master station (1995) (agrotechnical art 3) word) of effect of increasing production through whole nation agricultural technology extension master station of the Ministry of Agriculture.Whole nation agricultural technology extension service centre tested 20 various crop in 21 counties of 18 provinces (city, district) in nineteen ninety-five, what effect of increasing production was apparent in view accounts for 97.4%, great majority volume increase 10～30% (referring to No. 30 literary compositions of (1996) agrotechnical (grain and oil) letter word).
Claims (5)
1. one kind concentrates the method be processed into strain powder with microorganism manure strain agent, it is characterized in that spawn culture is fermented into gemma, concentrates through the flame filter press dehydration, is dried to gemma powder (10,000,000,000/gram) again in being lower than 80 ℃ of hot blasts.This method is applicable to the sporiferous microorganism manure strain of all energy, comprises fixed nitrogen sporeformer, phosphorus bacteria and potassium bacterium etc.
2. the sporiferous vinelandii of energy according to claim 1 are meant patent preservation bacterial classification bacillus azotobacter CCTCC M95040, it is characterized in that nitrogenase activity is strong and can produce gemma, can high temperature resistant (80 ℃) dry (water content＜5%), make and concentrate that to contain bacteria concentration height, anti-storage, transport applications behind the fixed nitrogen bacterial classification gemma powder convenient.
3. be meant bacillus megaterium AC-CC10010 according to the described phosphorus bacteria of claim 1, it is characterized in that transforming the soil insoluble phosphorus is available phosphorus, the gemma rate of formation is high and fast, and high temperature resistant drying is made the phosphorus bacteria strain powder that concentrates gemma and contained bacteria concentration height, anti-storage, transport applications conveniently.
4. potassium bacterium according to claim 1 is meant bacillusmusilaginosiengineering AC-CC10013, it is characterized in that energy activating soil insoluble potassium, the gemma rate of formation is high and high temperature resistant drying is made the potassium bacteria culture powder that concentrates gemma and contained the bacteria concentration height, and anti-storage, transport applications are convenient.
5. method as making microbial fertilizer composite bacteria powder as described in the right 1-4 is characterized in that it comprises following bacterial classification:
(1) the vinelandii gemma powder made of bacillus azotobacter CCTCC M95040;
(2) the phosphorus bacteria gemma powder made of bacillus megaterium ACCC10010;
(3) the potassium bacterial spore powder made of bacillusmusilaginosiengineering ACCC10013.
It is 10,000,000,000/gram that above composite bacteria powder contains the gemma number, and wherein vinelandii, phosphorus bacteria, potassium bacterium respectively account for 1/3.The ratio that this composite bacteria powder joins fertilizer matrix is 0.5-1%, and the particulate state microorganism organic composite fertilizer material of making contains hundred million of effective bacterium 0.5-1/gram.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN96104890A CN1098237C (en) | 1996-05-15 | 1996-05-15 | Concentrated strain powder for microbial fertilizer and its production |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN96104890A CN1098237C (en) | 1996-05-15 | 1996-05-15 | Concentrated strain powder for microbial fertilizer and its production |
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| Publication Number | Publication Date |
|---|---|
| CN1139092A true CN1139092A (en) | 1997-01-01 |
| CN1098237C CN1098237C (en) | 2003-01-08 |
Family
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN96104890A Expired - Fee Related CN1098237C (en) | 1996-05-15 | 1996-05-15 | Concentrated strain powder for microbial fertilizer and its production |
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| CN (1) | CN1098237C (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2009003341A1 (en) * | 2007-07-04 | 2009-01-08 | Qingli Yang | Composite chemical fertilizer comprising bioactive materials |
| CN102181389A (en) * | 2011-03-18 | 2011-09-14 | 大连民族学院 | Method for breeding Bacillaceae with high spore yield |
| CN107699518A (en) * | 2017-10-17 | 2018-02-16 | 临沂国龙生态科技有限公司 | A kind of preparation method of bacillus megaterium bacterium powder fertilizer |
| CN112375709A (en) * | 2020-11-18 | 2021-02-19 | 鹤壁市人元生物技术发展有限公司 | Binary microorganism composite microbial inoculum and preparation method thereof |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103254012B (en) * | 2013-05-24 | 2015-02-11 | 史丹利化肥股份有限公司 | Compound microbial slow release fertilizer specially used forpotatoes, and preparation method thereof |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1092397A (en) * | 1993-03-18 | 1994-09-21 | 中国科学院沈阳应用生态研究所 | Multi-component organic composite fertilizer and preparation method thereof |
| CN1107178A (en) * | 1994-11-14 | 1995-08-23 | 中国人民解放军军事医学科学院实验动物中心 | Bioligical fertilizer producing method and used microbe |
-
1996
- 1996-05-15 CN CN96104890A patent/CN1098237C/en not_active Expired - Fee Related
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2009003341A1 (en) * | 2007-07-04 | 2009-01-08 | Qingli Yang | Composite chemical fertilizer comprising bioactive materials |
| CN102181389A (en) * | 2011-03-18 | 2011-09-14 | 大连民族学院 | Method for breeding Bacillaceae with high spore yield |
| CN102181389B (en) * | 2011-03-18 | 2013-04-10 | 大连民族学院 | Method for breeding Bacillaceae with high spore yield |
| CN107699518A (en) * | 2017-10-17 | 2018-02-16 | 临沂国龙生态科技有限公司 | A kind of preparation method of bacillus megaterium bacterium powder fertilizer |
| CN112375709A (en) * | 2020-11-18 | 2021-02-19 | 鹤壁市人元生物技术发展有限公司 | Binary microorganism composite microbial inoculum and preparation method thereof |
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| Publication number | Publication date |
|---|---|
| CN1098237C (en) | 2003-01-08 |
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Owner name: INST OF AGRICULTURAL RESOURCES Free format text: FORMER OWNER: QI HONG Effective date: 20050805 |
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Effective date of registration: 20050805 Address after: 100081 No. 12 South Main Street, Haidian District, Beijing, Zhongguancun Patentee after: Institute of agricultural resources and regional planning, Chinese Academy of Agricultural Sciences Address before: 100081 transfer to Chen Tingwei, Institute of soil and fertilizer, Chinese Academy of Agricultural Sciences, 30 Whitehead Road, Beijing, Haidian District Patentee before: Qi Hong |
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