CN105176965A - Preparation method for bactericide and granulous compound soil conditioner prepared from bactericide - Google Patents
Preparation method for bactericide and granulous compound soil conditioner prepared from bactericide Download PDFInfo
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Abstract
The invention discloses a preparation method for a bactericide and a granulous compound soil conditioner prepared from the bactericide. The preparation method for the bactericide comprises the following steps that 1, strains are selected; 2, the selected actinomycetes, gram negative bacteria and white rot fungi are cultured in a large scale to obtain a large quantity of degrading bacteria; 3, the solid-state powder bactericide is prepared. The soil conditioner is prepared from 76%-80% of the solid-state powder bactericides, 7%-10% of nitrogenous fertilizer or calcium magnesia phosphate fertilizer or potash fertilizer or mixtures of several of the nitrogenous fertilizer, the calcium magnesia phosphate fertilizer and the potash fertilizer, 7%-10% of farmyard manure and 4%-6% of lanthanide microelements. The solid-state powder bactericide compounded with attapulgite and microorganisms can fully achieve the advantages of the attapulgite and the microorganisms, greatly improve the adsorbing property and adsorbing efficiency to organic pollutants, keep and maintain the soil environment and be treated on site and is suitable for treating soil pollution in a large area.
Description
Technical field
The invention belongs to green technology and technical field of land improvement, be specifically related to a kind of method utilizing Organic pollutants in the product degradation soil after the Attapulgite of microwave modification and the method for microorganism compound and compound, or a kind of lasting maintenance or increase the granulated fertilizer of soil fertility.
Background technology
Over 30 years, along with the mankind's activity of socioeconomic high speed development and high strength, China constantly expands because polluting the soil scope of degenerating, and soil quality worsens aggravation.Material and artificial the discharging pollutants such as a large amount of chemical fertilizer, agricultural chemicals is application of due to soil, the common problem such as made loss of soil nutrient, soil compaction, soil replace, thus directly have influence on grain yield and food safety, threaten the existence of people and healthy.According to national Soil Pollution Investigation result in 2014, China's edatope situation totally allowed of no optimist, and some areas soil pollution is heavier, and arable soil environmental quality causes anxiety, industry and mining discarded ground edatope outstanding problem.Within 2015, be " world's soil year ", add " ten, gas " and " ten, water " is issued in succession, when " the national Soil Pollution Control action plan " that be called as " ten, soil " is come out of the stove and is more caused the very big concern of environmental protection circle.This plan basic forming, wherein point out that China intends with about 5 years time containment soil pollution degradating trend, visible quickening advances the control of soil pollution very urgent.
At present, main pollutent comprises organic pesticide, dyestuff, PCBs and heavy metal etc.Compare heavy metal, the organic contamination of soil more extensively, more seriously and more complicated, is the focus that domestic and international soil and environmental protection circle are paid close attention to jointly.The method of administering soil pollution is numerous, has Physical, chemical method, biological process and physics, the chemical method etc. be combined with biophase.Physical cost is high and range of application is wideless; Chemical method cost is low, manipulative capability is strong, simple; Biological process low consumption, efficient, environmental safety, contaminant degradation are thorough; Chemical method and biological process conbined usage are at present for the focus of rehabilitating soil.Stabilization is the topmost technology of the current soil remediation of China, it is with the toxicity of poisonous and harmful component in chemical terms reduction pollutent, solvability and transport property, if add microbial method in this art, soil organic pollutant effect of then degrading is stronger, can high efficient recovery edatope, the compound use of these United Technologies more can the harm of comprehensive blocking-up soil pollutant.
Attapulgite is the high-quality sorbent material using this technology.Attapulgite, also known as polygorskite, is the clay pit to contain Shuifu County's magnesium silicate of a kind of layer of chain transition structure, has special filamentary structure.Fig. 1 is the SEM microgram of Attapulgite.Because its monocrystalline inside is pore passage structure, also numerous Parallel Tunnel spaces is naturally form between nano crystal fiber arranged in parallel, inside defines huge specific surface area, which dictates that the absorption property that it is good, can purify the organic pollutant in soil and improvement Soil structure.Microorganism can take organic pollutant as sole carbon source and the energy, and the Metabolic activity by microorganism that can be direct or indirect, is thoroughly converted into the natural process of simple, nontoxic inorganics by organic pollutant.Microbe species is numerous, and usual a kind of microorganism can be degraded Some Organic Pollutants, saves cost, process efficiently, not changes the multiple advantages such as edatope, is the focus of the various environmental problem of current process.
Although the method for current rehabilitating soil is numerous, its state of the art is generally on the low side, and soil improvement agent is of a great variety, but majority is powdery, receives certain limitation.At present, utilize Attapulgite to remove organic contaminants in soil and can improve Soil structure again, the patent strengthening soil fertility is less.Grant number be the Chinese patent of CN102935356B disclose a kind of utilize attapulgite modified method preparing compound adsorbent of poly aspartic acid and products thereof and application.Although poly aspartic acid has the advantages such as nontoxic, pollution-free, degradable but natural poly aspartic acid not easily obtains, the poly aspartic acid cost of chemosynthesis is relatively high, need high-temperature and high-pressure conditions, be unfavorable for energy-conserving and environment-protective, and often performance is single, not high to the adsorptive power of Organic Pollutants in Wastewater, utilize the organically-modified Attapulgite of poly aspartic acid compared to inorganic modified comparatively complicated simultaneously in this method, can not industrial production be widely used in.Grant number is that the Chinese patent of CN103159571B discloses a kind of soil water-reataining fertilizer-keeping agent and preparation method thereof.The sorbent material being specifically selected from the former powder of humic acid, zeolite powder, expanded soil or attapulgite You the biological water-keeping agent of γ – polyglutamic acid and one or more forms.The preserve moisture and fertility effect of this water-reataining fertilizer-keeping agent is better, but the pollutent in removal water body is limited, sorbent material in this agent is the undisturbed soil of non-modified, though have better absorption property but the ore deposit soil phase of its adsorptive power and modification reduces more greatly, thus also reduce the ability that pollutent removed by whole reagent.
Summary of the invention
The object of the invention is the shortcoming being to overcome prior art, the composite soil modifier a kind of preparation method of microbial inoculum being provided and adopting this bacterium to prepare, this microbial inoculum not only material cost is low, adsorptivity is strong, be applied in soil and effectively can improve Soil structure, eliminate soil organic contamination, and can soil fertility be strengthened, can extensively be applied to all kinds of farm crop, fertility effect is high.
Object of the present invention is realized by following scheme:
The preparation method of microbial inoculum of the present invention, it comprises the following steps:
(1) bacteria selection is carried out, detailed process is: get bacterium, fungi, actinomycetes join in microorganism reactor respectively, and add sterilized water and dilute, add substratum respectively again, phenyl-hexachloride, DDT, the mixing solutions of polychlorobiphenyl and enzyme, the temperature in microorganism reactor is regulated to reach 25 ~ 35 DEG C, pH is 6 ~ 8, mixing speed is 120 ~ 180r/min, aseptically carry out the cultivation of bacterial strain, then the concentration of organic pollutant in each reactor is detected, by the degradation rate of organic pollutant in the reactor that calculates often kind of bacterial strain place, obtain organic pollutant degradation rate relatively large, and phenyl-hexachloride of simultaneously degrading, DDT, the Institute of Micro-biology of this three type organic of polychlorobiphenyl reactor, and utilize the strain variety in this reactor of microscopic examination, be defined as actinomycetes, Gram-negative bacteria and whiterot fungi,
(2) actinomycetes filtered out, Gram-negative bacteria and whiterot fungi are carried out large scale culturing, obtain a large amount of degradation bacteria;
(3) thermally-denatured for microwave Attapulgite is carried out sterilizing 10 ~ 30min at 80 ~ 90 DEG C, as microbe carrier, then in described microbe carrier, add described degradation bacteria and add sterilized water and substratum, stir obtained mixing solutions, be put into again in freeze drying box, the massfraction of last moisture is made to control 13% ~ 17%, obtain solid powder microbial inoculum, described microwave thermal modified attapulgite, degradation bacteria, sterilized water and substratum are respectively 30% ~ 35% in the mass percent of mixing solutions, 5% ~ 10%, 20% ~ 30% and 25% ~ 40%,
Described microwave thermal modified attapulgite, concrete preparation process is as follows:
Get microwave thermal Attapulgite, be first heated to 200 ~ 400 DEG C and carry out high-temperature roasting, after 1 ~ 3h, cooling, grinding, then cross 200 ~ 300 mesh sieves; Again order number after screening is carried out microwave modification 5 ~ 15min, then centrifugation, washing at 200 ~ 300 object Attapulgite under 200 ~ 400Hz, 70 ~ 90 DEG C of conditions, dry at 100 ~ 110 DEG C, obtain the thermally-denatured Attapulgite of microwave.
The granular composite soil modifier of one of the present invention, each component concentration is as follows by mass percentage:
Solid powder microbial inoculum 76% ~ 80%
One or several mixture 7% ~ 10% in nitrogenous fertilizer, fused(calcium magnesium)phosphate or potash fertilizer
Farm manure 7% ~ 10%
Group of the lanthanides trace element 4% ~ 6%
Compared with existing soil restoring technology, the present invention has following advantage:
(1) Attapulgite rich reserves, cheap and easy to get, nontoxic non-stimulated, stable in properties, and modified Attapulgite significantly can remove all kinds of poisonous and hazardous organic pollutant in soil, and ep-type material, has a very wide range of application prospect.
(2) microwave modification can accelerate the heat transfer rate of Attapulgite inside, and homogeneous heating, shorten heat-up time, save energy; Microbial method reparation is efficient, and non-secondary pollution, thoroughly eliminates organic contamination; Solid powder microbial inoculum after Attapulgite and microorganism compound can give full play to the two advantage, not only greatly improve the absorption property to organic pollutant and adsorption efficiency, keep and safeguard edatope, and can original position treatment in situ, be suitable for large-area treatment soil pollution.
(3) healthy trace elements with household such as iodine, selenium, zinc in Attapulgite is not only contained in soil composite modifying agent, also has a small amount of group of the lanthanides trace element added in addition, can jointly give plant-growth necessary element, improve Soil structure, in addition the various organic fertilizer added in soil improvement agent, be beneficial to maintenance fertility, greatly facilitate the raising of plant biomass and various farm crop can be widely used in.
(4) this modifying agent is granular, can avoid the loss of nutritive ingredient, use facility, and production technique is simple, is convenient to scale operation.
Accompanying drawing explanation
Fig. 1 is the SEM microgram of Attapulgite;
Fig. 2 is the process flow sheet preparing granular composite soil modifier of the present invention.
Embodiment
Fig. 2 is process flow sheet of the present invention, elaborates further the present invention below by concrete case study on implementation.
The preparation method of microbial inoculum of the present invention, it comprises the following steps:
(1) bacteria selection is carried out, detailed process is: get bacterium, fungi, actinomycetes and join respectively in microorganism reactor, and add sterilized water and dilute, add substratum respectively again, the mixing solutions of phenyl-hexachloride, DDT, polychlorobiphenyl and enzyme, regulate the temperature in microorganism reactor to reach 25 ~ 35 DEG C, pH is 6 ~ 8, mixing speed is 120 ~ 180r/min, aseptically carries out the cultivation of bacterial strain.These bacterial strains have a kind of in degraded phenyl-hexachloride, DDT, these three kinds of organic pollutants of polychlorobiphenyl, two or three ability, detect the concentration of organic pollutant in each reactor, by the degradation rate of organic pollutant in the reactor that calculates often kind of bacterial strain place, then organic pollutant degradation rate is obtained relatively large, and the Institute of Micro-biology of phenyl-hexachloride of simultaneously degrading, DDT, this three type organic of polychlorobiphenyl reactor, and utilize the strain variety in this reactor of microscopic examination, be defined as actinomycetes, Gram-negative bacteria and whiterot fungi.
(2) (liquid fermentation technology can adopt fermentation technique disclosed in Wang Weiyun " edible fungus liquid submerged fermentation Research progress and prospect " actinomycetes filtered out, Gram-negative bacteria and whiterot fungi to be utilized liquid fermentation technology.) or (solid-state fermentation technology can adopt fermentation technique disclosed in Huang Daming " progress of solid-state fermentation technology and equipment thereof " to utilize solid-state fermentation technology.) carry out large scale culturing, obtain a large amount of degradation bacteria.
As one of the present invention preferred embodiment, the concrete steps of large scale culturing are: in 3 fermentor tanks, add natural medium and sterilized water respectively, add oxygenase again, then getting actinomycetes, Gram-negative bacteria and whiterot fungi puts in three fermentor tanks respectively, the mixing solutions adding phenyl-hexachloride, DDT and polychlorobiphenyl again in three fermentor tanks respectively cultivates 5 ~ 6 days obtained liquid microbial inoculums, aseptically utilize whizzer by the microorganism in fermented liquid and separation of fermentative broth, refilter, vacuum-drying, obtains degradation bacteria; Described natural medium, sterilized water, oxygenase and the mass percent of mixing solutions in liquid microbial inoculum be made up of phenyl-hexachloride, DDT and polychlorobiphenyl are respectively: 30% ~ 35%, 15% ~ 25%, 5% ~ 15% and 25% ~ 50%.
(3) Attapulgite of modification is carried out sterilizing 10 ~ 30min at 80 ~ 90 DEG C, as microbe carrier, then in described microbe carrier, add described degradation bacteria and add sterilized water and substratum, stir obtained mixing solutions, be put into again in freeze drying box, the massfraction of last moisture is made to control 13% ~ 17%, obtain solid powder microbial inoculum, described modified attapulgite, degradation bacteria, sterilized water and substratum are respectively 30% ~ 35%, 5% ~ 10%, 20% ~ 30% and 25% ~ 40% in the mass percent of mixing solutions;
Described microwave thermal modified attapulgite, concrete preparation process is as follows:
Get Attapulgite, be first heated to 200 ~ 400 DEG C and carry out high-temperature roasting, after 1 ~ 3h, cooling, grinding, then cross 200 ~ 300 mesh sieves; Again order number after screening is carried out microwave modification 5 ~ 15min at 200 ~ 300 object Attapulgite under 200 ~ 400Hz, 70 ~ 90 DEG C of conditions, then centrifugal point, washing, dry, obtain the thermally-denatured Attapulgite of microwave at 100 ~ 110 DEG C.
A kind of granular composite soil modifier, each component concentration is as follows by mass percentage:
Solid powder microbial inoculum 76% ~ 80%
One or several mixture 7% ~ 10% in nitrogenous fertilizer, fused(calcium magnesium)phosphate or potash fertilizer
Farm manure 7% ~ 10%
Group of the lanthanides trace element 4% ~ 6%
The preparation method of granular composite soil modifier, concrete steps are:
By above-mentioned mass percent, get nitrogenous fertilizer, fused(calcium magnesium)phosphate, potash fertilizer, farm manure and group of the lanthanides trace element: as lanthanum, cerium, neodymium etc. join in above-mentioned solid powder microbial inoculum, be under the condition of 30 ~ 40 DEG C in envrionment temperature, add water Homogeneous phase mixing again in solid powder microbial inoculum, the pH of mixing solutions is 6 ~ 8, then makes granular.Cool, dry, screening, obtains granular soil composite modifying agent.
Embodiment 1
(1) bacteria selection is carried out, detailed process is: get bacterium, fungi, actinomycetes and join respectively in microorganism reactor, and add sterilized water and dilute, add substratum respectively again, the mixing solutions of phenyl-hexachloride, DDT, polychlorobiphenyl and enzyme, regulate the temperature in microorganism reactor to reach 25 DEG C, pH is 6, mixing speed is 120r/min, aseptically carries out the cultivation of bacterial strain.These bacterial strains have a kind of in degraded phenyl-hexachloride, DDT, these three kinds of organic pollutants of polychlorobiphenyl, two or three ability, detect the concentration of organic pollutant in each reactor, by the degradation rate of organic pollutant in the reactor that calculates often kind of bacterial strain place, then organic pollutant degradation rate is obtained relatively large, and the Institute of Micro-biology of phenyl-hexachloride of simultaneously degrading, DDT, this three type organic of polychlorobiphenyl reactor, and utilize the strain variety in this reactor of microscopic examination, be defined as actinomycetes, Gram-negative bacteria and whiterot fungi.
(2) actinomycetes filtered out, Gram-negative bacteria and whiterot fungi are carried out large scale culturing, obtain a large amount of degradation bacteria.The concrete steps of large scale culturing are: in 3 fermentor tanks, add natural medium and sterilized water respectively, add oxygenase again, then getting actinomycetes, Gram-negative bacteria and whiterot fungi puts in three fermentor tanks respectively, the mixing solutions adding phenyl-hexachloride, DDT and polychlorobiphenyl again in three fermentor tanks respectively cultivates 5 days obtained liquid microbial inoculums, aseptically utilize whizzer by the microorganism in fermented liquid and separation of fermentative broth, refilter, vacuum-drying, obtain degradation bacteria; Described natural medium, sterilized water, oxygenase and the mass percent of mixing solutions in liquid microbial inoculum be made up of phenyl-hexachloride, DDT and polychlorobiphenyl are respectively: 30%, 25%, 5% and 40%.
(3) thermally-denatured for microwave Attapulgite is carried out sterilizing 30min at 80 DEG C, as microbe carrier, then in described microbe carrier, add described degradation bacteria and add sterilized water and substratum, stir obtained mixing solutions, be put into again in freeze drying box, the massfraction of last moisture is made to control 17%, obtain solid powder microbial inoculum, described modified attapulgite, degradation bacteria, sterilized water and substratum are respectively 30%, 5%, 30% and 35% in the mass percent of mixing solutions;
Described microwave thermal modified attapulgite, concrete preparation process is as follows:
Get Attapulgite, be first heated to 200 DEG C and carry out high-temperature roasting, after 1h, cooling, grinding, then cross 200 mesh sieves; Again order number after screening is carried out microwave modification 5min, then centrifugation, washing at 200 object Attapulgite under 400Hz, 70 DEG C of conditions, dry at 100 DEG C, obtain the thermally-denatured Attapulgite of microwave.
Solid powder microbial inoculum is to the effect detection experiment of removing soil organic contamination, and its concrete steps are:
The farmland planting district of China city suburbs taken from by sample, wherein be respectively 120ug/kg, 153ug/kg, 178ug/kg containing phenyl-hexachloride, DDT, polychlorobiphenyl, according to GB15618-2008 " standard of soil environment quality (revision) ", this three classes organic pollutant has exceeded Two-stage control standard all.
Weigh 4 parts of quality and be the above-mentioned soil sample of 10kg in beaker, be designated as A, B, C, D respectively.A is blank contaminated soil, respectively to adding unmodified Attapulgite in B, C, D, through the thermally-denatured Attapulgite of microwave, solid powder microbial inoculum.Add water appropriate on an equal basis respectively in this 4 increment basis, and mix, the content measuring organic pollutant in soil sample after 3 weeks is as follows:
After this process, though the residue content of organic contaminants in soil has still exceeded the Two-stage control standard of " standard of soil environment quality (revision) ", but there is degraded in a big way.
Granular composite soil modifier prepared by the solid powder microbial inoculum prepared with the present embodiment, and each component concentration is as follows by mass percentage:
Solid powder microbial inoculum 76%
Nitrogenous fertilizer 8%
Farm manure 10%
Trace element cerium 6%
By above-mentioned mass percent, get nitrogenous fertilizer, fused(calcium magnesium)phosphate, potash fertilizer, farm manure and group of the lanthanides trace element, joining in above-mentioned solid powder microbial inoculum, is under the condition of 30 DEG C in envrionment temperature, then the Homogeneous phase mixing that adds water in solid powder microbial inoculum, the pH of mixing solutions is 8, then makes granular.Cool, dry, screening, obtains granular soil composite modifying agent.
Granular composite soil modifier and common fertilizer are respectively used to maize planting, and the output of every mu of corn is as follows:
| Administration of material | The every per mu yield of corn (kg) |
| Granular composite soil modifier | 613 |
| Common fertilizer | 554 |
Test known, the adsorption of solid powder microbial inoculum is comparatively strong, then the interpolation granular composite soil modifier that above fertilizer, farm manure etc. are made facilitates the raising of corn yield significantly.
Embodiment 2
(1) bacteria selection is carried out, detailed process is: get bacterium, fungi, actinomycetes and join respectively in microorganism reactor, and add sterilized water and dilute, add substratum respectively again, the mixing solutions of phenyl-hexachloride, DDT, polychlorobiphenyl and enzyme, regulate the temperature in microorganism reactor to reach 35 DEG C, pH is 6.5, mixing speed is 135r/min, aseptically carries out the cultivation of bacterial strain.These bacterial strains have a kind of in degraded phenyl-hexachloride, DDT, these three kinds of organic pollutants of polychlorobiphenyl, two or three ability, detect the concentration of organic pollutant in each reactor, by the degradation rate of organic pollutant in the reactor that calculates often kind of bacterial strain place, then organic pollutant degradation rate is obtained relatively large, and the Institute of Micro-biology of phenyl-hexachloride of simultaneously degrading, DDT, this three type organic of polychlorobiphenyl reactor, and utilize the strain variety in this reactor of microscopic examination, be defined as actinomycetes, Gram-negative bacteria and whiterot fungi.
(2) actinomycetes filtered out, Gram-negative bacteria and whiterot fungi are carried out large scale culturing, obtain a large amount of degradation bacteria.The concrete steps of large scale culturing are: in 3 fermentor tanks, add natural medium and sterilized water respectively, add oxygenase again, then getting actinomycetes, Gram-negative bacteria and whiterot fungi puts in three fermentor tanks respectively, the mixing solutions adding phenyl-hexachloride, DDT and polychlorobiphenyl again in three fermentor tanks respectively cultivates 6 days obtained liquid microbial inoculums, aseptically utilize whizzer by the microorganism in fermented liquid and separation of fermentative broth, refilter, vacuum-drying, obtain degradation bacteria; Described natural medium, sterilized water, oxygenase and the mass percent of mixing solutions in liquid microbial inoculum be made up of phenyl-hexachloride, DDT and polychlorobiphenyl are respectively: 33%, 15%, 15% and 37%.
(3) thermally-denatured for microwave Attapulgite is carried out sterilizing 10min at 90 DEG C, as microbe carrier, then in described microbe carrier, add described degradation bacteria and add sterilized water and substratum, stir obtained mixing solutions, be put into again in freeze drying box, the massfraction of last moisture is made to control 13%, obtain solid powder microbial inoculum, described modified attapulgite, degradation bacteria, sterilized water and substratum are respectively 35%, 10%, 25% and 30% in the mass percent of mixing solutions;
Described microwave thermal modified attapulgite, concrete preparation process is as follows:
Get Attapulgite, be first heated to 300 DEG C and carry out high-temperature roasting, after 3h, cooling, grinding, then cross 250 mesh sieves; Again order number after screening is carried out microwave modification 15min, then centrifugation, washing at 250 object Attapulgite under 200Hz, 90 DEG C of conditions, dry at 110 DEG C, obtain the thermally-denatured Attapulgite of microwave.
Solid powder microbial inoculum is to the effect detection experiment of removing soil organic contamination, and its concrete steps are:
Weigh 1 part of quality be the sample identical with A soil in embodiment 1 of 10kg in beaker, be designated as E.In E, add the solid powder microbial inoculum prepared in the present embodiment, and add water and mix, the content measuring organic pollutant in soil sample after 3 weeks is as follows:
After this process, the residue content of organic contaminants in soil reaches the Two-stage control standard of " standard of soil environment quality (revision) ", and the degradation rate of organic pollutant now reaches 91% ~ 96%.
Granular composite soil modifier prepared by the solid powder microbial inoculum prepared with the present embodiment, and each component concentration is as follows by mass percentage:
Solid powder microbial inoculum 80%
The mixture 7% of nitrogenous fertilizer, fused(calcium magnesium)phosphate and potash fertilizer
Farm manure 8%
Trace element lanthanum 5%
The preparation method of the granular composite soil modifier of compound is with embodiment 1
Granular composite soil modifier and common fertilizer are respectively used to maize planting, and the output of every mu of corn is as follows:
| Administration of material | The every per mu yield of corn (kg) |
| Granular composite soil modifier | 659 |
| Common fertilizer | 554 |
Test known, the solid powder microbial inoculum adsorption prepared in the present embodiment not only strengthens to some extent, and its granular composite soil modifier facilitates again the raising of corn yield to a certain extent.
Embodiment 3
(1) bacteria selection is carried out, detailed process is: get bacterium, fungi, actinomycetes and join respectively in microorganism reactor, and add sterilized water and dilute, add substratum respectively again, the mixing solutions of phenyl-hexachloride, DDT, polychlorobiphenyl and enzyme, regulate the temperature in microorganism reactor to reach 30 DEG C, pH is 7, mixing speed is 145r/min, aseptically carries out the cultivation of bacterial strain.These bacterial strains have a kind of in degraded phenyl-hexachloride, DDT, these three kinds of organic pollutants of polychlorobiphenyl, two or three ability, detect the concentration of organic pollutant in each reactor, by the degradation rate of organic pollutant in the reactor that calculates often kind of bacterial strain place, then organic pollutant degradation rate is obtained relatively large, and the Institute of Micro-biology of phenyl-hexachloride of simultaneously degrading, DDT, this three type organic of polychlorobiphenyl reactor, and utilize the strain variety in this reactor of microscopic examination, be defined as actinomycetes, Gram-negative bacteria and whiterot fungi.
(2) actinomycetes filtered out, Gram-negative bacteria and whiterot fungi are carried out large scale culturing, obtain a large amount of degradation bacteria.The concrete steps of large scale culturing are: in 3 fermentor tanks, add natural medium and sterilized water respectively, add oxygenase again, then getting actinomycetes, Gram-negative bacteria and whiterot fungi puts in three fermentor tanks respectively, the mixing solutions adding phenyl-hexachloride, DDT and polychlorobiphenyl again in three fermentor tanks respectively cultivates 5.5 days obtained liquid microbial inoculums, aseptically utilize whizzer by the microorganism in fermented liquid and separation of fermentative broth, refilter, vacuum-drying, obtain degradation bacteria; Described natural medium, sterilized water, oxygenase and the mass percent of mixing solutions in liquid microbial inoculum be made up of phenyl-hexachloride, DDT and polychlorobiphenyl are respectively: 32%, 20%, 10% and 38%.
(3) thermally-denatured for microwave Attapulgite is carried out sterilizing 20min at 85 DEG C, as microbe carrier, then in described microbe carrier, add described degradation bacteria and add sterilized water and substratum, stir obtained mixing solutions, be put into again in freeze drying box, the massfraction of last moisture is made to control 14%, obtain solid powder microbial inoculum, described modified attapulgite, degradation bacteria, sterilized water and substratum are respectively 33%, 7%, 20% and 40% in the mass percent of mixing solutions;
Described microwave thermal modified attapulgite, concrete preparation process is as follows:
Get Attapulgite, be first heated to 328 DEG C and carry out high-temperature roasting, after 2h, cooling, grinding, then cross 300 mesh sieves; Again order number after screening is carried out microwave modification 10min, then centrifugation, washing at 300 object Attapulgite under 300Hz, 80 DEG C of conditions, dry at 105 DEG C, obtain the thermally-denatured Attapulgite of microwave.
Solid powder microbial inoculum is to the effect detection experiment of removing soil organic contamination, and its concrete steps are:
Weigh 1 part of quality be the soil sample identical with A in embodiment 1 of 10kg in beaker, be designated as F.In F, add the solid powder microbial inoculum prepared in the present embodiment, and add water and mix, the content measuring organic pollutant in soil sample after 3 weeks is as follows:
After this process, organic contaminants in soil again in a big way obtain degraded, its residue content reaches the Two-stage control standard of " standard of soil environment quality (revision) ", and wherein phenyl-hexachloride reaches the one-level control criterion of " standard of soil environment quality (revision) ".
Granular composite soil modifier prepared by the solid powder microbial inoculum prepared with the present embodiment, and each component concentration is as follows by mass percentage:
Solid powder microbial inoculum 79%
Fused(calcium magnesium)phosphate 10%
Farm manure 7%
Trace element lanthanum 4%
The preparation method of the granular composite soil modifier of compound is with embodiment 1
Granular composite soil modifier and common fertilizer are respectively used to maize planting, and the output of every mu of corn is as follows:
| Administration of material | The every per mu yield of corn (kg) |
| Granular composite soil modifier | 702 |
| Common fertilizer | 554 |
Test known, the adsorption of the solid powder microbial inoculum in the present embodiment is stronger compared with before, and the corn yield that its granular composite soil modifier is planted has had and improves greatly.
Embodiment 4
(1) bacteria selection is carried out, detailed process is: get bacterium, fungi, actinomycetes and join respectively in microorganism reactor, and add sterilized water and dilute, add substratum respectively again, the mixing solutions of phenyl-hexachloride, DDT, polychlorobiphenyl and enzyme, regulate the temperature in microorganism reactor to reach 32 DEG C, pH is 7.5, mixing speed is 160r/min, aseptically carries out the cultivation of bacterial strain.These bacterial strains have a kind of in degraded phenyl-hexachloride, DDT, these three kinds of organic pollutants of polychlorobiphenyl, two or three ability, detect the concentration of organic pollutant in each reactor, by the degradation rate of organic pollutant in the reactor that calculates often kind of bacterial strain place, then organic pollutant degradation rate is obtained relatively large, and the Institute of Micro-biology of phenyl-hexachloride of simultaneously degrading, DDT, this three type organic of polychlorobiphenyl reactor, and utilize the strain variety in this reactor of microscopic examination, be defined as actinomycetes, Gram-negative bacteria and whiterot fungi.
(2) actinomycetes filtered out, Gram-negative bacteria and whiterot fungi are carried out large scale culturing, obtain a large amount of degradation bacteria.The concrete steps of large scale culturing are: in 3 fermentor tanks, add natural medium and sterilized water respectively, add oxygenase again, then getting actinomycetes, Gram-negative bacteria and whiterot fungi puts in three fermentor tanks respectively, the mixing solutions adding phenyl-hexachloride, DDT and polychlorobiphenyl again in three fermentor tanks respectively cultivates 6 days obtained liquid microbial inoculums, aseptically utilize whizzer by the microorganism in fermented liquid and separation of fermentative broth, refilter, vacuum-drying, obtain degradation bacteria; Described natural medium, sterilized water, oxygenase and the mass percent of mixing solutions in liquid microbial inoculum be made up of phenyl-hexachloride, DDT and polychlorobiphenyl are respectively: 30%, 15%, 5% and 50%.
(3) thermally-denatured for microwave Attapulgite is carried out sterilizing 25min at 88 DEG C, as microbe carrier, then in described microbe carrier, add described degradation bacteria and add sterilized water and substratum, stir obtained mixing solutions, be put into again in freeze drying box, the massfraction of last moisture is made to control 15.5%, obtain solid powder microbial inoculum, described modified attapulgite, degradation bacteria, sterilized water and substratum are respectively 30%, 5%, 25% and 40% in the mass percent of mixing solutions;
Described microwave thermal modified attapulgite, concrete preparation process is as follows:
Get Attapulgite, be first heated to 400 DEG C and carry out high-temperature roasting, after 2.5h, cooling, grinding, then cross 230 mesh sieves; Again order number after screening is carried out microwave modification 13min, then centrifugation, washing at 230 object Attapulgite under 350Hz, 85 DEG C of conditions, dry at 107 DEG C, obtain the thermally-denatured Attapulgite of microwave.
Solid powder microbial inoculum is to the effect detection experiment of removing soil organic contamination, and its concrete steps are:
Weigh 1 part of quality be the soil sample identical with A in embodiment 1 of 10kg in beaker, be designated as G.In G, add the solid powder microbial inoculum prepared in the present embodiment, and add water and mix, the content measuring organic pollutant in soil sample after 3 weeks is as follows:
After this process, the pollutant load that in the present embodiment, the content of organic contaminants in soil is compared in embodiment 3 increases to some extent, but all within the Two-stage control standard of " standard of soil environment quality (revision) ".
Granular composite soil modifier prepared by the solid powder microbial inoculum prepared with the present embodiment, and each component concentration is as follows by mass percentage:
Solid powder microbial inoculum 76%
Potash fertilizer 10%
Farm manure 10%
Trace element lanthanum 4%
The preparation method of the granular composite soil modifier of compound is with embodiment 1
Granular composite soil modifier and common fertilizer are respectively used to maize planting, and the output of every mu of corn is as follows:
| Administration of material | The every per mu yield of corn (kg) |
| Granular composite soil modifier | 685 |
| Common fertilizer | 554 |
Test known, the solid powder microbial inoculum adsorptive power in the present embodiment decreases, and the corn yield of its granular composite soil modifier plantation is compared embodiment 3 and also decreased, but the corn amount of producing than common fertilizer is much higher.
Embodiment 5
(1) bacteria selection is carried out, detailed process is: get bacterium, fungi, actinomycetes and join respectively in microorganism reactor, and add sterilized water and dilute, add substratum respectively again, the mixing solutions of phenyl-hexachloride, DDT, polychlorobiphenyl and enzyme, regulate the temperature in microorganism reactor to reach 23 DEG C, pH is 8, mixing speed is 180r/min, aseptically carries out the cultivation of bacterial strain.These bacterial strains have a kind of in degraded phenyl-hexachloride, DDT, these three kinds of organic pollutants of polychlorobiphenyl, two or three ability, detect the concentration of organic pollutant in each reactor, by the degradation rate of organic pollutant in the reactor that calculates often kind of bacterial strain place, then organic pollutant degradation rate is obtained relatively large, and the Institute of Micro-biology of phenyl-hexachloride of simultaneously degrading, DDT, this three type organic of polychlorobiphenyl reactor, and utilize the strain variety in this reactor of microscopic examination, be defined as actinomycetes, Gram-negative bacteria and whiterot fungi.
(2) actinomycetes filtered out, Gram-negative bacteria and whiterot fungi are carried out large scale culturing, obtain a large amount of degradation bacteria.The concrete steps of large scale culturing are: in 3 fermentor tanks, add natural medium and sterilized water respectively, add oxygenase again, then getting actinomycetes, Gram-negative bacteria and whiterot fungi puts in three fermentor tanks respectively, the mixing solutions adding phenyl-hexachloride, DDT and polychlorobiphenyl again in three fermentor tanks respectively cultivates 5 days obtained liquid microbial inoculums, aseptically utilize whizzer by the microorganism in fermented liquid and separation of fermentative broth, refilter, vacuum-drying, obtain degradation bacteria; Described natural medium, sterilized water, oxygenase and the mass percent of mixing solutions in liquid microbial inoculum be made up of phenyl-hexachloride, DDT and polychlorobiphenyl are respectively: 35%, 25%, 15% and 25%.
(3) thermally-denatured for microwave Attapulgite is carried out sterilizing 15min at 83 DEG C, as microbe carrier, then in described microbe carrier, add described degradation bacteria and add sterilized water and substratum, stir obtained mixing solutions, be put into again in freeze drying box, the massfraction of last moisture is made to control 13.8%, obtain solid powder microbial inoculum, described modified attapulgite, degradation bacteria, sterilized water and substratum are respectively 35%, 10%, 30% and 25% in the mass percent of mixing solutions;
Described microwave thermal modified attapulgite, concrete preparation process is as follows:
Get Attapulgite, be first heated to 385 DEG C and carry out high-temperature roasting, after 1.7h, cooling, grinding, then cross 280 mesh sieves; Again order number after screening is carried out microwave modification 6min at 280 object Attapulgite under 250Hz, 75 DEG C of conditions, then centrifugal point, washing, dry, obtain the thermally-denatured Attapulgite of microwave at 103 DEG C.
Solid powder microbial inoculum is to the effect detection experiment of removing soil organic contamination, and its concrete steps are:
Weigh 1 part of quality be the soil sample identical with A in embodiment 1 of 10kg in beaker, be designated as H.In H, add the solid powder microbial inoculum of this enforcement preparation, and add water and mix, after 3 weeks, in mensuration soil sample, the content of organic pollutant is as follows:
After this process, the content of organic contaminants in soil is compared and is wanted many in embodiment 3, but all within the Two-stage control standard of " standard of soil environment quality (revision) ".Granular composite soil modifier prepared by the solid powder microbial inoculum prepared with the present embodiment, and each component concentration is as follows by mass percentage:
Solid powder microbial inoculum 76%
The mixture 10% of nitrogenous fertilizer, fused(calcium magnesium)phosphate and potash fertilizer
Farm manure 9%
Trace element neodymium 5%
Granular composite soil modifier and common fertilizer are respectively used to maize planting with embodiment 1 by the preparation method of the granular composite soil modifier of compound, and the output of every mu of corn is as follows:
| Administration of material | The every per mu yield of corn (kg) |
| Granular composite soil modifier | 581 |
| Common fertilizer | 554 |
Test known, the solid powder microbial inoculum prepared in the present embodiment has certain adsorption strength, and the output of its granular composite soil modifier maize planting is compared embodiment 3 and greatly reduced, but compares the raising that common fertilizer also has less degree.
Above embodiment has carried out concrete description to method of the present invention; but content of the present invention is not restricted to above case study on implementation; as long as not exceeding in main scope of the present invention; can change flexibly experiment condition and method, these all belong to protection scope of the present invention.Therefore, patent of the present invention and protection domain should be as the criterion with appended claims.
Claims (4)
1. a preparation method for microbial inoculum, is characterized in that, it comprises the following steps:
(1) bacteria selection is carried out, detailed process is: get bacterium, fungi, actinomycetes join in microorganism reactor respectively, and add sterilized water and dilute, add substratum respectively again, phenyl-hexachloride, DDT, the mixing solutions of polychlorobiphenyl and enzyme, the temperature in microorganism reactor is regulated to reach 25 ~ 35 DEG C, pH is 6 ~ 8, mixing speed is 120 ~ 180r/min, aseptically carry out the cultivation of bacterial strain, then the concentration of organic pollutant in each reactor is detected, by the degradation rate of organic pollutant in the reactor that calculates often kind of bacterial strain place, obtain organic pollutant degradation rate relatively large, and phenyl-hexachloride of simultaneously degrading, DDT, the Institute of Micro-biology of this three type organic of polychlorobiphenyl reactor, and utilize the strain variety in this reactor of microscopic examination, be defined as actinomycetes, Gram-negative bacteria and whiterot fungi,
(2) actinomycetes filtered out, Gram-negative bacteria and whiterot fungi are carried out large scale culturing, obtain a large amount of degradation bacteria;
(3) thermally-denatured for microwave Attapulgite is carried out sterilizing 10 ~ 30min at 80 ~ 90 DEG C, as microbe carrier, then in described microbe carrier, add described degradation bacteria and add sterilized water and substratum, stir obtained mixing solutions, be put into again in freeze drying box, the massfraction of last moisture is made to control 13% ~ 17%, obtain solid powder microbial inoculum, described microwave thermal modified attapulgite, degradation bacteria, sterilized water and substratum are respectively 30% ~ 35% in the mass percent of mixing solutions, 5% ~ 10%, 20% ~ 30% and 25% ~ 40%,
Described microwave thermal modified attapulgite, concrete preparation process is as follows:
Get microwave thermal Attapulgite, be first heated to 200 ~ 400 DEG C and carry out high-temperature roasting, after 1 ~ 3h, cooling, grinding, then cross 200 ~ 300 mesh sieves; Again order number after screening is carried out microwave modification 5 ~ 15min, then centrifugation, washing at 200 ~ 300 object Attapulgite under 200 ~ 400Hz, 70 ~ 90 DEG C of conditions, dry at 100 ~ 110 DEG C, obtain the thermally-denatured Attapulgite of microwave.
2. the preparation method of microbial inoculum according to claim 1, it is characterized in that: the concrete steps of the large scale culturing in described step (2) are: in 3 fermentor tanks, add natural medium and sterilized water respectively, add oxygenase again, then actinomycetes are got, Gram-negative bacteria and whiterot fungi are put in three fermentor tanks respectively, phenyl-hexachloride is added respectively again in three fermentor tanks, the mixing solutions of DDT and polychlorobiphenyl cultivates 5 ~ 6 days obtained liquid microbial inoculums, aseptically utilize whizzer by the microorganism in fermented liquid and separation of fermentative broth, refilter, vacuum-drying, obtain degradation bacteria, described natural medium, sterilized water, oxygenase and the mass percent of mixing solutions in liquid microbial inoculum be made up of phenyl-hexachloride, DDT and polychlorobiphenyl are respectively: 30% ~ 35%, 15% ~ 25%, 5% ~ 15% and 25% ~ 50%.
3. a granular composite soil modifier, is characterized in that: each component concentration is as follows by mass percentage:
Solid powder microbial inoculum 76% ~ 80%
One or several mixture 7% ~ 10% in nitrogenous fertilizer, fused(calcium magnesium)phosphate or potash fertilizer
Farm manure 7% ~ 10%
Group of the lanthanides trace element 4% ~ 6%
4. granular composite soil modifier according to claim 3, is characterized in that: described group of the lanthanides trace element is the one in lanthanum, cerium or neodymium.
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