CN113876667A

CN113876667A - Compound artemisia rupestris composition with skin brightening, anti-allergy and relieving effects and preparation method thereof

Info

Publication number: CN113876667A
Application number: CN202111352640.5A
Authority: CN
Inventors: 普珍; 袁瑞瑛; 卓玛东智; 王聚乐; 李斌; 黄山
Original assignee: Tibet University
Current assignee: Tibet University
Priority date: 2021-11-16
Filing date: 2021-11-16
Publication date: 2022-01-04
Anticipated expiration: 2041-11-16
Also published as: CN113876667B

Abstract

本发明公开了一种具有提亮肤色和抗敏舒缓作用的复方五脉绿绒蒿组合物及其制备方法，该组合物由以下重量份原料制成：五脉绿绒蒿10‑50份、红景天10‑50份和余甘子10‑50份。本发明还包括上述组合物的制备方法和应用。本发明的组合物用在护肤品中提亮肤色和抗敏舒缓作用，对皮肤作用更温和、无刺激性、安全性更高。The invention discloses a compound M. echinacea composition with skin-brightening, anti-allergic and soothing effects and a preparation method thereof. The composition is prepared from the following raw materials in parts by weight: 10-50 parts of M. echinacea, 10‑50 servings of Rhodiola rosea and 10‑50 servings of Amla. The present invention also includes the preparation method and application of the above-mentioned composition. The composition of the present invention is used in skin care products to brighten the skin and have anti-sensitivity and soothing effects, and has milder, non-irritating and safer skin effects.

Description

Compound artemisia rupestris composition with skin brightening, anti-allergy and relieving effects and preparation method thereof

Technical Field

The invention relates to the technical field of fine daily necessities, in particular to a compound artemisia pentandra composition with skin brightening, anti-allergy and relieving effects and a preparation method thereof.

Background

Skin color, which is the color of the skin, is the color of the epidermis of the human skin as reflected by pigmentation of the epidermis of the human skin, such as melanin, protopanarubicin, and phyllanthin. Skin color is distributed differently in different areas and populations. Skin color is related to the content and distribution (granular or dispersed) of melanin in the skin. Melanin is concentrated in and among the cells of the germinal layer of the epidermis. The dermis is generally devoid of melanin, but with pigments, it can penetrate the skin and appear cyan, such as gray-cyan patches of the sacral and buttocks of a newborn. In addition, the color of the skin is also related to the blood in the capillaries, the roughness and the moisturization of the skin.

Most Asian women want to have bright and white skin, but under the conditions that the work and life rhythm of the modern society is accelerated and the pressure is increased, many Asian women have the skin health problems of dull and dull skin and increased skin darkness, which causes troubles to the daily life and work of the Asian women. Thus, the need for skin lightening products is also an urgent requirement for asian female skin care products.

With the frequent use of various skin care and cosmetic products and the increase of external adverse stimuli such as environmental pollution, the proportion of people with sensitive skin is increasing. Sensitive skin is easy to have symptoms of reddening, fever, pruritus, stabbing pain and the like, and skin redness and swelling and rash can occur in severe cases. Sensitive skin also causes the problems of skin thinning, stratum corneum insufficiency, easy desquamation, poor water retention capacity, tight and dry skin and the like. With the increasing quality requirements of skin care and cosmetic products, consumers also want products that are milder, non-irritating, and safer to the skin.

At present, skin care products which can brighten skin color and resist allergy and relieve allergy are still rare. The products with the skin color brightening function mostly adopt vitamin C, tartaric acid, kojic acid, tretinoin and the like, the raw materials are easy to cause the problems of red swelling, fever, pruritus, stabbing pain and the like of sensitive skin, and the products do not brighten the skin color but cause new skin problems. Therefore, consumers want the product to have both effects, and a new solution is provided for solving the skin problem.

Disclosure of Invention

Aiming at the defects in the prior art, the invention provides the compound artemisia pentandra linn composition with the functions of brightening the skin and resisting allergy and relieving and the preparation method thereof.

In order to achieve the purpose, the technical scheme adopted by the invention for solving the technical problems is as follows: provides a compound artemisia rupestris composition with skin brightening, anti-allergy and relieving effects, which is prepared from the following raw materials in parts by weight: 10-50 parts of Wumai green wormwood, 10-50 parts of rhodiola rosea and 10-50 parts of emblic leafflower fruit.

Further, the compound artemisia rupestris composition with the skin brightening, anti-allergy and relieving effects is prepared from the following raw materials in parts by weight: 20-40 parts of Wumai green wormwood, 20-40 parts of rhodiola rosea and 20-40 parts of emblic leafflower fruit.

The herba Artemisiae Annuae is whole plant of Meconopsis quintufolia Regel. Has cold property, sweet and astringent taste, and has effects of clearing heat, promoting urination, diminishing inflammation, and relieving pain, and can be used for treating pneumonia, hepatitis, heat syndrome of liver and lung, and edema. Mainly contains alkaloids such as papaverine, norsanguinarine, and WUMAILAOHAOJIA, and flavonoids such as luteolin, quercetin, and dihydroquercetin.

The radix Rhodiolae is dry root and rhizome of Rhodiola crenulata Rhodiola crenulata (hook.f. et Thoms.) H.Ohba. Has neutral nature, sweet and bitter taste, has effects of invigorating qi, promoting blood circulation, dredging collaterals and relieving asthma, and can be used for treating qi deficiency, blood stasis, thoracic obstruction, cardiodynia, apoplexy, hemiplegia, listlessness and asthma. Mainly contains salidroside, tyrosol, etc.

Fructus Phyllanthi is dry mature fruit of Phyllanthus emblica L. of Euphorbiaceae. Has cold property, sweet, sour and astringent taste, has effects of clearing heat and cooling blood, resolving food stagnation and invigorating stomach, promoting fluid production and relieving cough, and can be used for treating blood heat and blood stasis, dyspepsia, abdominal distention, cough, laryngalgia, and dry mouth. Mainly contains gallic acid, ellagic acid, rutin, quercetin, etc.

The preparation method of the compound artemisia ciliaris composition with the effects of brightening the skin and resisting allergy and relieving the skin comprises the following steps in sequence:

(1) weighing dried Artemisia quinquefolius, rhodiola rosea and emblic leafflower fruit, mixing, crushing, and sieving by a 20-mesh sieve to obtain mixed powder;

(2) adding water into the mixed powder obtained in the step (1) according to the volume mass ratio of 10-30:1, reflux-extracting for 1-3 times for 1-3h each time, filtering, mixing filtrates, and concentrating under normal pressure to obtain fluid extract;

(3) adding water into the clear paste obtained in the step (2) until the concentration is 0.5-2g/mL, then adding 95% ethanol until the alcohol content reaches 50-85%, standing overnight, taking the filtered supernatant, and drying under reduced pressure to obtain the compound artemisia pentandra linn composition with the effects of brightening the skin, resisting allergy and relieving.

Further, in the step (2), the volume-to-mass ratio is 20: 1.

The compound artemisia selengensis composition with the skin brightening, anti-allergy and relieving effects is applied to preparing skin care products with the skin brightening, anti-allergy and relieving effects.

Furthermore, the compound artemisia rupestris composition with the effects of brightening the skin and resisting allergy and relieving the skin is added in an amount of 0.1-10 wt%.

Furthermore, the compound artemisia rupestris composition with the effects of brightening the skin and resisting allergy and relieving the skin is added in an amount of 1-8 wt%.

Further, the skin care product is a face wash, a face cream, a soap, a cream, an emulsion, water, a mask or a gel.

In summary, the invention has the following advantages:

the compound artemisia selengensis composition disclosed by the invention has the effects of brightening the skin and resisting allergy and relieving, can be used for lightening darkness and improving skin sensitivity, can be used for daily skin care products, and can meet the use requirements of various consumers. The invention takes the traditional Tibetan medicine theory as guidance and combines with the modern scientific and technological research results, and the artemisia ciliaris, the rhodiola rosea and the emblic leafflower fruit are used in a matching way, so that the invention has the functions of brightening the skin color, lightening the darkness, resisting the allergy, relieving and improving the skin sensitivity, and can be used for daily skin care products.

Detailed Description

Example 1

A compound artemisia rupestris composition with skin brightening, anti-allergy and relieving effects is prepared from the following raw materials in parts by weight: 40 parts of Wumai green wormwood, 40 parts of red scenery and 20 parts of emblic leafflower fruit.

(2) adding water into the mixed powder obtained in the step (1) according to the volume mass ratio of 15:1, reflux-extracting for 2 times, 2 hours each time, filtering, combining the filtrates, and concentrating under normal pressure to obtain fluid extract;

(3) adding water into the clear paste obtained in the step (2) until the concentration is 1g/mL, then adding 95% ethanol until the alcohol content reaches 70%, standing overnight, taking the filtered supernatant, and drying under reduced pressure to obtain the compound artemisia pentandra and artemisia scoparia composition with the effects of brightening the skin, resisting allergy and relieving.

Example 2

A compound artemisia rupestris composition with skin brightening, anti-allergy and relieving effects is prepared from the following raw materials in parts by weight: 40 parts of Wumai green wormwood, 30 parts of rhodiola rosea and 30 parts of emblic leafflower fruit.

(2) adding water into the mixed powder obtained in the step (1) according to the volume mass ratio of 30:1, reflux-extracting for 1 time, 3 hours each time, filtering, combining the filtrates, and concentrating under normal pressure to obtain fluid extract;

(3) adding water into the clear paste obtained in the step (2) until the concentration is 1.5g/mL, then adding 95% ethanol until the alcohol content reaches 60%, standing overnight, taking the filtered supernatant, and drying under reduced pressure to obtain the compound artemisia pentandra linn composition with the effects of brightening the skin, resisting allergy and relieving.

Example 3

A compound artemisia rupestris composition with skin brightening, anti-allergy and relieving effects is prepared from the following raw materials in parts by weight: 40 parts of Wumai green wormwood, 20 parts of rhodiola rosea and 40 parts of emblic leafflower fruit.

(2) adding water into the mixed powder obtained in the step (1) according to the volume mass ratio of 10:1, reflux-extracting for 3 times, each time for 1h, filtering, mixing filtrates, and concentrating under normal pressure to obtain fluid extract;

(3) adding water into the clear paste obtained in the step (2) until the concentration is 0.5g/mL, then adding 95% ethanol until the alcohol content reaches 85%, standing overnight, taking the filtered supernatant, and drying under reduced pressure to obtain the compound artemisia pentandra linn composition with the effects of brightening the skin, resisting allergy and relieving.

Example 4

A compound artemisia rupestris composition with skin brightening, anti-allergy and relieving effects is prepared from the following raw materials in parts by weight: 30 parts of Wumai green wormwood, 30 parts of rhodiola rosea and 40 parts of emblic leafflower fruit.

(2) adding water into the mixed powder obtained in the step (1) according to the volume mass ratio of 20:1, reflux-extracting for 2 times, each time for 1.5h, filtering, mixing filtrates, and concentrating under normal pressure to obtain fluid extract;

(3) adding water into the clear paste obtained in the step (2) until the concentration is 2g/mL, then adding 95% ethanol until the alcohol content reaches 50%, standing overnight, taking the filtered supernatant, and drying under reduced pressure to obtain the compound artemisia pentandra and artemisia scoparia composition with the effects of brightening the skin, resisting allergy and relieving.

Example 5

A compound artemisia rupestris composition with skin brightening, anti-allergy and relieving effects is prepared from the following raw materials in parts by weight: 20 parts of Wumai green wormwood, 40 parts of rhodiola rosea and 40 parts of emblic leafflower fruit.

(2) adding water into the mixed powder obtained in the step (1) according to the volume mass ratio of 25:1, reflux-extracting for 2 times, each time for 2.5h, filtering, mixing filtrates, and concentrating under normal pressure to obtain fluid extract;

(3) adding water into the clear paste obtained in the step (2) until the concentration is 1g/mL, then adding 95% ethanol until the alcohol content reaches 75%, standing overnight, taking the filtered supernatant, and drying under reduced pressure to obtain the compound artemisia pentandra and artemisia scoparia composition with the effects of brightening the skin, resisting allergy and relieving.

Comparative example 1

Comparative example 1 differs from example 1 in that only Artemisia quinquefolius was included.

Comparative example 2

Comparative example 2 differs from example 1 in that only rhodiola rosea was contained.

Comparative example 3

Comparative example 3 differs from example 1 in that only emblic leafflower fruit is contained.

Comparative example 4

Comparative example 4 differs from example 1 in that it does not contain emblic leafflower fruit.

Comparative example 5

Comparative example 5 differs from example 1 in that rhodiola rosea is not included.

Comparative example 6

Comparative example 6 differs from example 1 in that Artemisia quinquefolius is not included.

Examples of the experiments

The compositions obtained in example 1 and comparative examples 1 to 6 were used to prepare corresponding skin creams according to the formulation shown in Table 1.

TABLE 1 skin cream formulation table

Heating the components of group A and B to 70 deg.C, dissolving completely, and mixing well. Slowly adding group A into group B under stirring, slowly cooling to 30 deg.C, adding group C, stirring, and cooling to room temperature to obtain skin cream. The skin cream of each proportion is prepared according to the same method.

1. Comparative experiment for skin color brightening effect

(1) A subject: selecting 70 subjects, female, 20-45 years old and indoor workers, wherein the exclusion conditions of the subjects meet the inclusion and exclusion standards of the cosmetic contact dermatitis diagnosis standard and treatment principle; no serious systemic disease, no immunodeficiency or autoimmune disease; an inactive allergic disease; no allergic history to skin care cosmetics; hormone medicine and immunosuppressant have not been used systemically in 1 month, and are not in gestation or lactation period; no ethical contraindication; skin color was tested between II-IV using healthy subjects according to Chardon's grouping. The subjects were randomly divided into 7 groups of 10 persons each, and skin color lightening effect comparison experiments were performed using the skin cream prepared by the composition of example 1 and the above comparative example.

(2) Testing indexes are as follows: skin whiteness (brightness) (L)^*Value): l is^*The value is white balance, and the color is more white when the value is larger, and is more black when the value is smaller.

Skin brightness (dark yellowness) (ITA ° value): ITA DEG value is the skin individual type angle, is equal to L^*Value b and^*value-dependent values characterizing skin brightness. The greater the ITA value, the brighter the skin, whereas the darker the skin.

The test method comprises the following steps: selecting the inner sides of the left arm crank and the right arm crank of the testee as tested areas (with the same area), randomly marking, setting blank areas (without using any sample), smearing experimental samples on the tested areas for 1 time in the morning and at night every day, and smearing the experimental samples according to personal use habits. The use period was 8 weeks. The subjects did not change the brand and habit of daily use of the cleaning product during the test. After 8 weeks of use of the test product, data were collected from the test site using a test instrument (Mexameter MX 18 skin melanin and heme tester, MPA9 multifunctional skin tester and colorimetric test probe, CK company, germany).

And (3) data analysis: if the data are normally distributed, the variation of the data before and after the use of the sample and the difference between the test area and the matrix area are analyzed by using the t-test. If the data are distributed in a non-normal way, corresponding statistical analysis is carried out by applying rank sum test, and a conclusion whether the data are effective or not is obtained according to a calculation result. The data calculation formulas are shown in formula (1) and formula (2).

Formula (1) Δ L^*Value of L^*Value (test area) -L^*Value (blank), formula (2) Δ ITA ° value ═ ITA ° value (test area) -ITA ° (blank).

Skin whiteness (brightness) (L)^*Values) the results are shown in table 2.

TABLE 2 skin whiteness (brightness) (L)^*Value) measurement result

Product group	L^*Value (test area)	L^*Value (blank space)	ΔL^*Value of
				Example 1	72.28±3.35	61.17±2.62	11.11±2.07
Comparative example 1	62.39±2.55^**	61.58±3.86	0.81±0.30^**
				Comparative example 2	68.06±2.80^**	61.88±2.43	6.18±2.21^**
Comparative example 3	64.56±2.72^**	60.09±3.38	4.47±1.76^**
				Comparative example 4	68.33±2.81^*	62.30±2.71	6.03±2.56^**
Comparative example 5	67.01±2.70^**	61.27±3.50	5.74±1.29^**
				Comparative example 6	68.29±3.85^*	60.72±3.18	7.57±2.15^**

Note: in comparison with the example 1, the present inventors have conducted a comparison,^*P<0.05，^**P<0.01。

as can be seen from Table 1, useExample 1 product of the invention after 8 weeks, subject test area L^*The value is obviously increased, and compared with various comparative products, the value is obviously different (P)<0.05，P<0.01). Delta L of the product of example 1 of the invention in comparison with the blank zone of the same subject^*The value is also significantly higher than that of the other comparative products (P)<0.01). The formula of the invention has obvious synergistic skin color brightening effect, and compared with a comparative product containing only one or two raw materials, the formula of the invention has obvious skin color brightening effect and definite synergistic effect.

2. Anti-allergic and antipruritic test

After 1 week of acclimatization of 90 mice, they were randomly divided into 9 groups: blank control group, histamine group, inventive example 1, comparative examples 1-6, 10 per group. In the experiment, 1% sodium sulfide is used for removing the hair of the mouse back, the area of the skin area for removing the hair is 2cm multiplied by 3cm, and the mouse is used for the experiment after 2 days. In the experiment, the back hairless area of each group of animals is locally coated with corresponding samples (0.2 ml/mouse) for 5 days continuously, and the neck and the back of each group of mice are injected with 50 mu L/mouse intracutaneously after 1h of the last administration except for a blank control group. After histamine administration, mice were observed for itching and the number of scratching was recorded within 30 min. The test data were statistically analyzed using SPSS 22.0 statistical software, and the statistics were expressed as mean. + -. standard deviation, and if the data were normally distributed, the variation of the data before and after the use of the sample and the difference between the test area and the matrix area were analyzed using the t-test method. If the data are distributed in a non-normal way, corresponding statistical analysis is carried out by applying rank sum test, and a conclusion whether the data are effective or not is obtained according to a calculation result. The results of the anti-allergic and antipruritic tests are shown in Table 3.

TABLE 3 anti-allergic antipruritic test results

Product group	Number of scratching itch within 30min
		Blank group	18.50±2.17^##
Histamine group	96.38±30.77^**
		Example 1	25.80±10.51^*##
Comparative example 1	55.49±17.80^**##
		Comparative example 2	70.66±23.35^**#
Comparative example 3	69.52±18.83^**#
		Comparative example 4	50.73±17.20^**##
Comparative example 5	46.69±14.31^**##
		Comparative example 6	70.52±23.27^**#

Note: in comparison with the blank set, the results,^*P<0.05,^**P<0.01. in comparison with the histamine group,^#P<0.05，^##P<0.01。

as can be seen from Table 3, the product of the example of the invention has obvious anti-allergic itching relieving effect on itch caused by histamine, and has a significant difference (P <0.01) compared with histamine model group, although the itch scratching frequency is still higher than that of blank group, compared with other comparative example group, the itch scratching frequency of the product of the example 1 is obviously lower than that of other comparative example groups, and the itching relieving effect is obvious. The formula of the invention has obvious synergistic anti-allergy and relieving effects, and compared with a comparative product containing only one or two raw materials, the formula of the invention has obvious anti-allergy and itching relieving effects and definite synergistic effect.

While the present invention has been described in detail with reference to the specific embodiments thereof, it should not be construed as limited by the scope of the present patent. Various modifications and changes may be made by those skilled in the art without inventive step within the scope of the appended claims.

Claims

1. a compound recipe M. echinacea composition with brightening complexion and anti-allergic soothing effect, is characterized in that, is made of following parts by weight raw materials: 10-50 parts of M. cortex 50 servings and Emilia 10-50 servings.

2. the compound recipe M. eelonite with brightening complexion and anti-allergic and soothing effect as claimed in claim 1, is characterized in that, is made of following raw materials in parts by weight: 20-40 parts of M. echinacea, 20-40 servings of Rhodiola rosea and 20-40 servings of Amla.

3. the preparation method of the compound Meconium elegans composition with brightening complexion and anti-allergic soothing effect according to any one of claims 1-2, is characterized in that, comprises the following steps successively:

(1) take by weighing dry M. chinensis, Rhodiola Rhodiola and Emblica emblica, pulverize after mixing, cross 20 mesh sieves to obtain mixed powder;

(2) adding water to the mixed powder obtained in step (1) at a volume-to-mass ratio of 10-30:1, refluxing for 1-3 times, 1-3h each time, filtering, and merging the filtrates and concentrating at normal pressure to obtain a clear paste;

(3) adding water to the clear paste obtained in step (2) to a concentration of 0.5-2g/mL, then adding 95% ethanol to make the alcohol content reach 50-85%, leaving it to stand overnight, taking the filtered supernatant, reducing the pressure For drying, the compound Meconium elegans composition with brightening, anti-allergic and soothing effects is obtained.

4. The preparation method of the compound Meconium elegans composition with brightening skin color and anti-allergic and soothing effects as claimed in claim 3, characterized in that, in step (2), the volume-to-mass ratio is 20:1.

5. The application of the compound Meconopsis quinquefasciata composition with skin-brightening and anti-allergic soothing effects according to any one of claims 1-2 in preparing skin care products with skin-brightening and anti-allergic soothing effects.

6 . The application according to claim 5 , characterized in that, the addition amount of the compound M. echinacea composition with brightening skin color and anti-allergic and soothing effects is 0.1-10 wt %. 7 .

7 . The application according to claim 5 , characterized in that, the addition amount of the compound M. echinacea composition with brightening skin color and anti-allergic and soothing effects is 1-8 wt %. 8 .

8. The application according to claim 5, wherein the skin care product is a facial cleanser, facial cleanser, soap, ointment, cream, lotion, water, facial mask or gel.