CN113846029A - Application method of high-temperature aerobic fermentation biological agent with agricultural solid waste as matrix - Google Patents
Application method of high-temperature aerobic fermentation biological agent with agricultural solid waste as matrix Download PDFInfo
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- CN113846029A CN113846029A CN202111040970.0A CN202111040970A CN113846029A CN 113846029 A CN113846029 A CN 113846029A CN 202111040970 A CN202111040970 A CN 202111040970A CN 113846029 A CN113846029 A CN 113846029A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B09—DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
- B09B—DISPOSAL OF SOLID WASTE
- B09B3/00—Destroying solid waste or transforming solid waste into something useful or harmless
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
- C05F17/20—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation using specific microorganisms or substances, e.g. enzymes, for activating or stimulating the treatment
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/02—Separating microorganisms from their culture media
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
Abstract
The invention relates to the technical field of agricultural solid waste recycling, in particular to an application method of a high-temperature aerobic fermentation biological agent by taking agricultural solid waste as a substrate, which aims to solve the problems of low high-temperature aerobic fermentation temperature, long fermentation period, incomplete harmlessness and serious odor pollution and comprises the following steps: s1, uniformly mixing the agricultural solid wastes to form an organic material with the water content of 40-60%; s2, collecting a sample in a high-temperature environment of Tengchong volcanic entrance, and culturing and separating multiple strains of the sample at 80-90 ℃; s3, streak culture, and selecting dominant strains; s4, performing amplification culture on the dominant strains by using the organic materials in the step S1; s5, inoculating the dominant strains which are subjected to the amplification culture in the step S4 into the organic material in the step S1; s6, adding the organic material inoculated in the step S5 into a fermentation tank, and blowing air to the inside by using a fan to form rotten clinker; and S7, continuously inoculating other strains to the decomposed material to form bacterial manure of different types.
Description
Technical Field
The invention relates to the technical field of agricultural solid waste recycling, in particular to an application method of a high-temperature aerobic fermentation biological agent with agricultural solid waste as a matrix.
Background
Agricultural solid waste is a general term for waste discharged in agricultural production, agricultural product processing, livestock and poultry breeding industry and rural residents. The agricultural solid waste is mainly from waste generated in plant cultivation industry, agricultural and sideline product processing industry and animal breeding industry and the life of rural residents. The method comprises the following steps of: farmland and fruit residues such as straw, stubble, weeds, fallen leaves, fruit hulls, vines, branches and other waste; processing waste of agricultural products; livestock and poultry manure and pen bedding; human excrement and urine and domestic waste. Common agricultural solid wastes include rice straws, wheat straws, corn straws, rice hulls, rhizomes, fallen leaves, fruit peels, fruit pits, feathers, fur, livestock and poultry feces, dead livestock and rural domestic garbage and the like. Comprehensive utilization of agricultural wastes with the same body is required; can not be used, collected, stored and disposed according to the national relevant environmental protection regulations, and can prevent the environment from being polluted.
The agricultural solid waste contains a large amount of organic solid waste, such as livestock and poultry breeding waste, the organic solid waste is rich in N, P, K and nutrients such as organic matters, and the current organic solid waste treatment method mainly comprises landfill, incineration, anaerobic digestion, aerobic fermentation treatment and the like, wherein high-temperature aerobic fermentation is an important means for harmless treatment and resource utilization of the organic waste, but the traditional high-temperature aerobic fermentation technology also has the defects of low fermentation temperature, long fermentation period, incomplete harmlessness and serious odor pollution.
Disclosure of Invention
In order to solve the technical problems, the invention provides an application method of a high-temperature aerobic fermentation biological agent by taking agricultural solid waste as a substrate, and aims to solve the problems of low high-temperature aerobic fermentation temperature, long fermentation period, incomplete harmlessness and serious odor pollution.
The invention relates to an application method of a high-temperature aerobic fermentation biological agent by taking agricultural solid waste as a substrate, which comprises the following steps:
s1, uniformly mixing the agricultural solid waste, then crushing, spraying brown sugar solution with the weight being 5% of that of the agricultural solid waste, and then drying to form organic materials with the water content being 40-60% for later use;
s2, collecting a sample in a high-temperature environment of Tengchong volcanic, culturing the sample in an environment of 80-90 ℃, taking the organic materials in the step S1 as a culture medium, culturing for 20 days, diluting and coating, and separating multiple strains;
s3, carrying out streak culture on the multiple strains in the step S2 by adopting the organic materials in the step S1, and selecting dominant strains;
s4, performing amplification culture on the dominant strains by using the organic materials in the step S1;
s5, inoculating the dominant strains which are subjected to the amplification culture in the step S4 into the organic material in the step S1, wherein the inoculation ratio is 2-3%;
s6, adding the organic materials inoculated in the step S5 into a fermentation tank, blowing air to the inside by using a fan, periodically turning the pile, and continuously fermenting for 15-20 days to form rotten clinker;
and S7, continuously inoculating other strains to the decomposed material to form bacterial manure of different types.
The invention discloses an application method of a high-temperature aerobic fermentation biological agent with agricultural solid waste as a matrix, wherein the concentration of a brown sugar solution in the step S1 is 20%.
According to the application method of the high-temperature aerobic fermentation biological agent with the agricultural solid waste as the matrix, the decomposed material accounting for 5-10% of the weight of the organic material is added into the organic material in the step S5.
The invention discloses an application method of a high-temperature aerobic fermentation biological agent by taking agricultural solid waste as a matrix, which comprises the following steps of: turning the piles once every 3 days in the early stage, and turning the piles once every 2 days in the later stage.
The invention discloses an application method of a high-temperature aerobic fermentation biological agent by taking agricultural solid waste as a substrate, wherein the ventilation quantity in the step S6 is 10-30 cubic/(ton hour).
The invention relates to an application method of a high-temperature aerobic fermentation biological agent with agricultural solid waste as a matrix, wherein the agricultural solid waste is livestock and poultry breeding waste, rice straw, wheat straw, corn straw, rice hull, rhizome, fallen leaves and fruit peel.
Compared with the prior art, the invention has the beneficial effects that: firstly, the sample of will getting from the crater carries out various cultivations, selects high temperature resistant advantage bacterial, carries out fermentation treatment to organic solid waste through high temperature resistant advantage bacterial, improves the temperature among the fermentation process, shortens the fermentation period, can not produce the gas of serious harm environment simultaneously to improved the degradation rate to organic solid waste, be fit for promoting.
Drawings
FIG. 1 is a graph of fermentation temperatures for different water cut conditions for a first set of examples;
FIG. 2 is a graph of fermentation temperature for a second set of examples, with different aeration levels.
Detailed Description
The following detailed description of embodiments of the present invention is provided in connection with the accompanying drawings and examples. The following examples are intended to illustrate the invention but are not intended to limit the scope of the invention.
As shown in fig. 1 to fig. 2, the method for applying the high-temperature aerobic fermentation biological agent with agricultural solid waste as a substrate comprises the following steps:
s1, uniformly mixing the agricultural solid waste, then crushing, spraying a brown sugar solution with the weight of 5% of that of the agricultural solid waste, wherein the concentration of the brown sugar solution is 20%, and then drying to form an organic material with the water content of 40-60% for later use;
s2, collecting a sample in a high-temperature environment of Tengchong volcanic, culturing the sample in an environment of 80-90 ℃, taking the organic materials in the step S1 as a culture medium, culturing for 20 days, diluting and coating, and separating multiple strains;
s3, carrying out streak culture on the multiple strains in the step S2 by adopting the organic materials in the step S1, and selecting dominant strains;
s4, performing amplification culture on the dominant strains by using the organic materials in the step S1;
s5, inoculating the dominant strains which are subjected to the amplification culture in the step S4 into the mixture of the organic materials and the decomposed materials in the step S1, wherein the inoculation proportion is 2-3%, and the weight of the decomposed materials is 5-10% of that of the organic materials;
s6, adding the organic materials inoculated in the step S5 into a fermentation tank, blowing air to the inside by using a fan, wherein the ventilation rate is 10-30 cubic/(ton hour), and periodically turning the piles, wherein the piles are turned once every 3 days in the early stage and once every 2 days in the later stage, and the fermentation is continued for 15-20 days to form rotten materials;
and S7, continuously inoculating other strains to the decomposed material to form bacterial manure of different types.
A first set of examples: testing the water content of the organic material;
| numbering | A1 | A2 | A3 |
| Water content (%) | 40 | 50 | 60 |
A1, A2 and A3 all carry out the following steps:
s1, uniformly mixing the agricultural solid waste, then crushing, spraying a brown sugar solution with the weight of 5% of that of the agricultural solid waste, wherein the concentration of the brown sugar solution is 20%, and then drying to form an organic material for later use;
s2, collecting a sample in a high-temperature environment of Tengchong volcanic, culturing the sample in an environment of 80-90 ℃, taking the organic materials in the step S1 as a culture medium, culturing for 20 days, diluting and coating, and separating multiple strains;
s3, carrying out streak culture on the multiple strains in the step S2 by adopting the organic materials in the step S1, and selecting dominant strains;
s4, performing amplification culture on the dominant strains by using the organic materials in the step S1;
s5, inoculating the dominant strains which are subjected to the amplification culture in the step S4 into the mixture of the organic materials and the decomposed materials in the step S1, wherein the inoculation proportion is 2.5%, and the weight of the decomposed materials is 6.9% of that of the organic materials;
s6, adding the organic materials inoculated in the step S5 into a fermentation tank, blowing air to the inside by using a fan, wherein the ventilation rate is 20 cubic/(ton hour), and periodically turning the piles, wherein the piles are turned once every 3 days in the early stage, the total number of the piles is 3, the piles are turned once every 2 days in the later stage, the total number of the piles is 4, and the fermentation is continued for 17 days to form rotten clinker;
and S7, continuously inoculating other strains to the decomposed material to form bacterial manure of different types.
The data in FIG. 1 were obtained, and the fermentation temperature was higher in the case of the water content of 40% and 50% based on the data in FIG. 1.
Second group of embodiments: testing the ventilation quantity;
| numbering | B1 | B2 | B3 |
| Air flow cubic/(ton hour) | 10 | 20 | 30 |
B1, B2 and B3 are all carried out by the following steps:
s1, uniformly mixing the agricultural solid waste, then crushing, spraying a brown sugar solution with the weight of 5% of that of the agricultural solid waste, wherein the concentration of the brown sugar solution is 20%, and then drying to form an organic material with the water content of 50% for later use;
s2, collecting a sample in a high-temperature environment of Tengchong volcanic, culturing the sample in an environment of 80-90 ℃, taking the organic materials in the step S1 as a culture medium, culturing for 20 days, diluting and coating, and separating multiple strains;
s3, carrying out streak culture on the multiple strains in the step S2 by adopting the organic materials in the step S1, and selecting dominant strains;
s4, performing amplification culture on the dominant strains by using the organic materials in the step S1;
s5, inoculating the dominant strains which are subjected to the amplification culture in the step S4 into the mixture of the organic materials and the decomposed materials in the step S1, wherein the inoculation proportion is 3%, and the weight of the decomposed materials is 6.9% of that of the organic materials;
s6, adding the organic materials inoculated in the step S5 into a fermentation tank, blowing air to the inside by using a fan, and periodically turning the piles, wherein the piles are turned once every 3 days in the early stage and 3 times in total, and the piles are turned once every 2 days in the later stage and 4 times in total, and continuously fermenting for 17 days to form rotten clinker;
and S7, continuously inoculating other strains to the decomposed material to form bacterial manure of different types.
The data in figure 2 were obtained and from the data in figure 2, the fermentation temperature was higher at an aeration rate of 20/(tonnes hour).
Example 3:
the following steps are carried out:
s1, uniformly mixing the agricultural solid waste, then crushing, spraying a brown sugar solution with the weight of 5% of that of the agricultural solid waste, wherein the concentration of the brown sugar solution is 20%, and then drying to form an organic material with the water content of 50% for later use;
s2, collecting a sample in a high-temperature environment of Tengchong volcanic, culturing the sample in an environment of 80-90 ℃, taking the organic materials in the step S1 as a culture medium, culturing for 20 days, diluting and coating, and separating multiple strains;
s3, carrying out streak culture on the multiple strains in the step S2 by adopting the organic materials in the step S1, and selecting dominant strains;
s4, performing amplification culture on the dominant strains by using the organic materials in the step S1;
s5, inoculating the dominant strains which are subjected to the amplification culture in the step S4 into the mixture of the organic materials and the decomposed materials in the step S1, wherein the inoculation proportion is 3%, and the weight of the decomposed materials is 6.9% of that of the organic materials;
s6, adding the organic materials inoculated in the step S5 into a fermentation tank, blowing air to the inside by using a fan, wherein the air flow is 20/(ton hour), and periodically turning the piles, wherein the piles are turned once every 3 days in the early stage and are counted for 3 times, and the piles are turned once every 2 days in the later stage and are counted for 4 times, and continuously fermenting for 17 days to form rotten clinker;
and S7, continuously inoculating other strains to the decomposed material to form bacterial manure of different types.
Comparative example:
the traditional aerobic fermentation mode is used.
From example 3 and the comparative example, the following data were obtained:
according to the data, the method disclosed by the invention has the advantages that the highest fermentation temperature is high, the fermentation time is shortened, only ammonia gas is generated, and meanwhile, the degradation rate of the organic solid waste can reach 73%.
For the decomposed material prepared in example 3, bacteria were isolated, genomic DNA was extracted, and then 16s rDNA fragments were PCR amplified, and compared with GeneBank or Eztaxon to obtain strains: hyperthermophiles, geobacillus and methylotrophic bacillus.
The above description is only a preferred embodiment of the present invention, and it should be noted that, for those skilled in the art, several modifications and variations can be made without departing from the technical principle of the present invention, and these modifications and variations should also be regarded as the protection scope of the present invention.
Claims (6)
1. An application method of a high-temperature aerobic fermentation biological agent taking agricultural solid waste as a substrate is characterized by comprising the following steps:
s1, uniformly mixing the agricultural solid waste, then crushing, spraying brown sugar solution with the weight being 5% of that of the agricultural solid waste, and then drying to form organic materials with the water content being 40-60% for later use;
s2, collecting a sample in a high-temperature environment of Tengchong volcanic, culturing the sample in an environment of 80-90 ℃, taking the organic materials in the step S1 as a culture medium, culturing for 20 days, diluting and coating, and separating multiple strains;
s3, carrying out streak culture on the multiple strains in the step S2 by adopting the organic materials in the step S1, and selecting dominant strains;
s4, performing amplification culture on the dominant strains by using the organic materials in the step S1;
s5, inoculating the dominant strains which are subjected to the amplification culture in the step S4 into the organic material in the step S1, wherein the inoculation ratio is 2-3%;
s6, adding the organic materials inoculated in the step S5 into a fermentation tank, blowing air to the inside by using a fan, periodically turning the pile, and continuously fermenting for 15-20 days to form rotten clinker;
and S7, continuously inoculating other strains to the decomposed material to form bacterial manure of different types.
2. The method for applying the high-temperature aerobic fermentation biological agent with agricultural solid waste as the matrix as claimed in claim 1, wherein the concentration of the brown sugar solution in the step S1 is 20%.
3. The method for applying the high-temperature aerobic fermentation biological agent with agricultural solid waste as the matrix as claimed in claim 2, wherein the decomposed material in an amount of 5-10% by weight of the organic material is further added to the organic material in the step S5.
4. The method for applying the high-temperature aerobic fermentation biological agent with agricultural solid waste as the matrix according to claim 3, wherein the pile-turning method in the step S6 comprises the following steps: turning the piles once every 3 days in the early stage, and turning the piles once every 2 days in the later stage.
5. The method for applying the high-temperature aerobic fermentation biological agent based on agricultural solid waste as claimed in claim 4, wherein the ventilation amount in the step S6 is 10-30 cubic/(ton hour).
6. The method for applying the high-temperature aerobic fermentation biological agent by using the agricultural solid waste as the matrix according to claim 5, wherein the agricultural solid waste is livestock and poultry breeding waste, rice straw, wheat straw, corn straw, rice hull, rhizomes, fallen leaves and fruit peels.
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1955276A (en) * | 2006-09-04 | 2007-05-02 | 利耀铭 | Organic microbial composite and use |
| CN108033817A (en) * | 2017-11-27 | 2018-05-15 | 福建农林大学 | A kind of method of antibiotic and resistant gene in quick abatement organic solid waste |
| CN110550987A (en) * | 2019-09-18 | 2019-12-10 | 同济大学 | Method for treating organic solid waste and biological organic fertilizer prepared by method |
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- 2021-09-07 CN CN202111040970.0A patent/CN113846029A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1955276A (en) * | 2006-09-04 | 2007-05-02 | 利耀铭 | Organic microbial composite and use |
| CN108033817A (en) * | 2017-11-27 | 2018-05-15 | 福建农林大学 | A kind of method of antibiotic and resistant gene in quick abatement organic solid waste |
| CN110550987A (en) * | 2019-09-18 | 2019-12-10 | 同济大学 | Method for treating organic solid waste and biological organic fertilizer prepared by method |
Non-Patent Citations (1)
| Title |
|---|
| 廖汉鹏: "有机固体废物超高温好氧发酵技术及其工程应用", 《福建农林大学学报(自然科学版)》, vol. 46, no. 4, pages 439 - 444 * |
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