CN113842363A

CN113842363A - Preparation process and application of Chinese pulsatilla root particles for livestock and poultry

Info

Publication number: CN113842363A
Application number: CN202111278271.XA
Authority: CN
Inventors: 杨成祥; 赵云生; 赵蕾; 范新蕾; 司俊伟; 孟令昭
Original assignee: Henan Feilaite Biotechnology Co ltd; Henan Muyou Animal Husbandry Technical Service Co ltd; Henan Narisen Biotechnology Co ltd; Henan Narisen Biopharmaceutical Co ltd
Current assignee: Henan Feilaite Biotechnology Co ltd; Henan Muyou Animal Husbandry Technical Service Co ltd; Henan Narisen Biotechnology Co ltd; Henan Narisen Biopharmaceutical Co ltd
Priority date: 2021-10-30
Filing date: 2021-10-30
Publication date: 2021-12-28

Abstract

The invention relates to a preparation process and application of Chinese pulsatilla root granules for livestock and poultry, which are prepared from the following medicaments in part by weight: the Chinese pulsatilla root granule is prepared from 400-800 g of Chinese pulsatilla root, 150-300 g of coptis root, 450-1000 g of ash bark and 300-600 g of golden cypress by taking the four traditional Chinese medicines as raw materials, crushing, decocting for multiple times, mixing decoction solutions, filtering, precipitating with ethanol, recovering ethanol, concentrating filtrate, performing reduced pressure spray drying to obtain an extract dry paste, crushing the extract dry paste at the temperature of below 60 ℃, adding a proper amount of two or more of cyclodextrin, starch, silica gel micropowder, lactose, glucose, microcrystalline cellulose and the like, uniformly mixing, and preparing into granules by using a low-temperature boiling drying granulator to obtain the Chinese pulsatilla root granule. The Chinese pulsatilla root granules for livestock and poultry have the advantages of high content of effective components, no drug residue and low drug cost, can be used for large-scale cultivation and mixed drinking for drug administration, are convenient to store and transport, accord with the characteristics of a new veterinary drug preparation, and are suitable for preventing and treating intestinal infectious diseases of digestive systems of livestock and poultry.

Description

Preparation process and application of Chinese pulsatilla root particles for livestock and poultry

Technical Field

The invention relates to a traditional Chinese veterinary medicine preparation with the effect of preventing and treating intestinal infectious diseases of digestive tract systems of livestock and poultry, and a preparation process and application thereof, in particular to a preparation process and application of Chinese pulsatilla root particles for livestock and poultry, and belongs to the field of traditional Chinese medicine preparations.

Background

Infectious diseases of the digestive tract system are one of the most common diseases affecting livestock and poultry breeding, particularly mixed infection, secondary infection and drug-resistant bacteria infection appear in recent years, the characteristics of high morbidity, complicated disease sources, difficult control of conventional medicines and the like are presented, serious loss is brought to the breeding industry, the livestock and poultry digestive system infection is clinically mainly manifested as digestive system dysfunction, enteritis, diarrhea, yellow-white dysentery, proventriculitis, myogastritis, enterotoxin syndrome and the like, pathogeny is mainly caused by pathogenic escherichia coli, and the pathogeny is infected through the digestive tract. Is a common disease and frequently encountered disease of livestock and poultry, and almost all livestock and poultry farms suffer from the diseases. This disease can occur all the year round, but it is common in early spring, severe winter and hot summer. Is one of several diseases which afflict the breeder. The infection of the digestive system of the livestock and poultry causes physiological dysfunction and produces various inflammatory reactions of the digestive system, and the like. The digestive system diseases of the livestock and poultry are mainly caused by the infection of the livestock and poultry with various pathogenic microorganisms. At present, chemically synthesized antibiotic drugs are commonly used for treating the diseases, and the drug residue is serious; the prescription of the Chinese pulsatilla root granules for livestock and poultry is derived from Chinese pulsatilla root decoction from Shanghai treatise on typhoid fever, and the Chinese pulsatilla root granules consist of 4 medicines of Chinese pulsatilla root, coptis root, phellodendron bark and ash bark and have the effects of clearing away heat and toxic materials, cooling blood and stopping dysentery; it is mainly used for treating dysentery with diarrhea, abdominal pain, tenesmus, purulent blood, burning anus, yellow tongue coating, and wiry and rapid pulse. Is a classic prescription for clinically treating diseases such as dysentery, chronic ulcerative colitis, colitis and the like. The 'Chinese pulsatilla root oral liquid' is recorded in 'Chinese veterinary pharmacopoeia' of 2015 edition, but the common Chinese pulsatilla root decoction and the Chinese pulsatilla root oral liquid have the defects of large use amount in clinical application, inconvenient use, inconvenient administration conditions and storage and transportation of oral Chinese pulsatilla root decoction or Chinese pulsatilla root oral liquid, high use cost and the like. The Chinese pulsatilla root granules for livestock and poultry are subjected to dosage form improvement by a modern preparation technology, and a preparation method of a novel dosage form granule conforming to the modern medicine technology is developed, is extract powder extracted from pure traditional Chinese medicines, has higher content of active ingredients, better water solubility, small dosage and low cost, is suitable for mixed feeding and mixed drinking administration in large-scale cultivation, has the advantages of difficult generation of drug resistance, no drug residue and the like, and is a necessary good medicine for clinically treating livestock and poultry with digestive system diseases.

Disclosure of Invention

The invention aims to provide a Chinese pulsatilla root granule for livestock and poultry, the prescription of which is prepared from Chinese pulsatilla root decoction (Chinese pulsatilla root, coptis root, phellodendron bark and ash bark) and Chinese pulsatilla root oral liquid, and the Chinese pulsatilla root granule is a common prescription for treating heat diarrhea and dysentery. In the formula, the Chinese pulsatilla root is a key medicine for treating diarrhea and dysentery, and has the effects of clearing heat, cooling blood and removing toxicity; the coptis, the phellodendron and the ash bark are bitter and cold in property, dry dampness and clear and purge triple energizer, and the four medicines are used together to have the functions of clearing heat, removing toxicity, eliminating dampness and stopping dysentery. According to the modern medical research, the Chinese pulsatilla root, the coptis root and the golden cypress have obvious inhibiting effect on dysentery bacilli, escherichia coli, staphylococcus and the like, have better dysentery treating effect, are compatible and applied, complement each other, have more obvious effect on treating livestock and poultry piglet white dysentery, colibacillosis, enteritis and other digestive tract diseases, are more convenient to use, store and transport, and have low cost.

The invention relates to a preparation process and application of Chinese pulsatilla root granules for livestock and poultry, and provides a veterinary medicinal granule preparation for treating intestinal infection of digestive systems of livestock and poultry, which mainly comprises the following components in parts by weight: 400-800 g of Chinese pulsatilla root, 150-300 g of coptis root, 450-1000 g of ash bark and 300-600 g of phellodendron; the four components of the Chinese pulsatilla root, the coptis root, the ash bark, the golden cypress and the like are crushed into small pieces for later use. The method is characterized in that: the method comprises the following steps:

step (1): decocting the 4 medicines in water for 3 times, wherein 10 times of water is added for 1 hour/time each time, combining decoctions, filtering, and concentrating the filtrate until the relative density is 1.20-1.25 (70-80 ℃);

step (2): adding ethanol into the concentrated solution obtained in the step (1) to enable the ethanol content to reach 75%, standing for 12 hours, filtering to obtain a supernatant, adding a proper amount of 75% ethanol into residues, standing for 12 hours, filtering, combining ethanol solutions, recovering ethanol, adjusting the pH value to 5.0, refrigerating for 72 hours, filtering, and adjusting the pH value of the filtrate to 5.0;

and (3): concentrating the filtrate obtained in the step (2), and performing reduced pressure spray drying to obtain dry extract for later use;

and (4): pulverizing the extract dry extract at below 60 deg.C, adding appropriate amount of two or more adjuvants such as cyclodextrin, starch, silica gel micropowder, lactose, glucose, microcrystalline cellulose, etc., mixing, and granulating with low temperature boiling drying granulator to obtain radix Pulsatillae granule for livestock and fowl. Each g is equivalent to 2g of crude drug, and the using amount is as follows: drinking water or mixing materials: 1-1.5 g of Chinese pulsatilla root particles for livestock and poultry are added into 1L of water of the poultry for 3-5 days continuously. Is suitable for preventing and treating intestinal infectious diseases of digestive systems of livestock and poultry.

The Chinese pulsatilla root granules for livestock and poultry are fed to the livestock and poultry in a drinking water administration mode to prevent and treat digestive system diseases of the livestock and poultry. The veterinary drug composition of the invention applies the principle of traditional veterinary medical science and methods and selects the drugs according with the state of illness on the basis of dialectical legislation, and aims to meet the requirements of complex state of illness and convenient transportation, storage and administration of drugs and exert the synergistic effect of the drugs.

Detailed Description

The invention is described below with reference to examples, the embodiment schemes described herein are not intended to limit the invention, and those skilled in the art can make modifications and variations thereto according to the spirit of the invention, and all such modifications and variations are considered to be within the scope of the invention.

It should be noted that, in view of the needs in large-scale cultivation, the farmer may choose to administer the drug as a mixed drink or as a mixed feed. The Chinese pulsatilla root granules for livestock and poultry are a common prescription for treating heat diarrhea and dysentery in veterinary medicine clinic. In the formula, the Chinese pulsatilla root is a key medicine for treating diarrhea and dysentery, and has the effects of clearing heat, cooling blood and removing toxicity; the coptis, the phellodendron and the ash bark are bitter and cold in property, dry dampness and clear and purge triple energizer, and the four medicines are used together to have the functions of clearing heat, removing toxicity, eliminating dampness and stopping dysentery. According to the modern medical research, the Chinese pulsatilla root, the coptis root and the golden cypress have obvious inhibiting effect on dysentery bacilli, escherichia coli, staphylococcus and the like, have better dysentery treating effect, are compatible and applied, complement each other, have more obvious effect on treating livestock and poultry piglet white dysentery, colibacillosis, enteritis and other digestive tract diseases, and are more convenient to use, store and transport.

Example 1:

is prepared from the following medicaments in part by weight: 400-800 g of Chinese pulsatilla root, 150-300 g of coptis root, 450-1000 g of ash bark and 300-600 g of phellodendron; the four components of the Chinese pulsatilla root, the coptis root, the ash bark, the golden cypress and the like are crushed into small pieces for later use. The method is characterized in that: the method comprises the following steps: step (1): decocting the 4 medicines in water for 3 times, wherein 10 times of water is added for 1 hour/time each time, combining decoctions, filtering, and concentrating the filtrate until the relative density is 1.20-1.25 (70-80 ℃); step (2): adding ethanol into the concentrated solution obtained in the step (1) to enable the ethanol content to reach 75%, standing for 12 hours, filtering to obtain a supernatant, adding a proper amount of 75% ethanol into residues, standing for 12 hours, filtering, combining ethanol solutions, recovering ethanol, adjusting the pH value to 5.0, refrigerating for 72 hours, filtering, and adjusting the pH value of the filtrate to 5.0; and (3): concentrating the filtrate obtained in the step (2), and performing reduced pressure spray drying to obtain dry extract for later use; and (4): pulverizing the extract dry extract at below 60 deg.C, adding appropriate amount of two or more adjuvants such as cyclodextrin, starch, silica gel micropowder, lactose, glucose, microcrystalline cellulose, etc., mixing, and granulating with low temperature boiling drying granulator to obtain radix Pulsatillae granule for livestock and fowl. Each g is equivalent to 1.3-2.7 g of the original medicine, and the using amount is as follows: drinking water or mixing materials: 1-1.5 g of Chinese pulsatilla root particles for livestock and poultry are added into 1L of water of the poultry for 3-5 days continuously. Is suitable for preventing and treating digestive tract infectious diseases of livestock and poultry.

Example 2:

is prepared from the following medicaments in part by weight: 400g of Chinese pulsatilla root, 150g of coptis root, 450g of ash bark and 300g of phellodendron; the four components of the Chinese pulsatilla root, the coptis root, the ash bark, the golden cypress and the like are crushed into small pieces for later use. The Chinese pulsatilla root granules for livestock and poultry are obtained according to the steps as described in example 1, each g of the Chinese pulsatilla root granules is equal to 1.3g of the original medicine, and the using amount is as follows: drinking water or mixing materials: 1-1.5 g of Chinese pulsatilla root particles for livestock and poultry are added into 1L of water of the poultry for 3-5 days continuously. Is suitable for preventing and treating intestinal infectious diseases of digestive systems of livestock and poultry.

Example 3:

is prepared from the following medicaments in part by weight: 800g of Chinese pulsatilla root, 300g of coptis root, 1000g of ash bark and 600g of phellodendron; the four components of the Chinese pulsatilla root, the coptis root, the ash bark, the golden cypress and the like are crushed into small pieces for later use. The Chinese pulsatilla root granules are obtained according to the steps as described in example 1, each g of the Chinese pulsatilla root granules is equivalent to 2.7g of crude drug, and the using amount is as follows: drinking water or mixing materials: 1-1.5 g of Chinese pulsatilla root particles for livestock and poultry are added into 1L of water of the poultry for 3-5 days continuously. Is suitable for preventing and treating intestinal infectious diseases of digestive systems of livestock and poultry.

Example 4:

is prepared from the following medicaments in part by weight: 600g of Chinese pulsatilla root, 225g of coptis root, 725g of ash bark and 450g of phellodendron; the four components of the Chinese pulsatilla root, the coptis root, the ash bark, the golden cypress and the like are crushed into small pieces for later use. The Chinese pulsatilla root granules for livestock and poultry are obtained according to the steps as described in example 1, each g of the Chinese pulsatilla root granules is equivalent to 2g of crude drug, and the using amount is as follows: drinking water or mixing materials: 1-1.5 g of Chinese pulsatilla root particles for livestock and poultry are added into 1L of water of the poultry for 3-5 days continuously. Is suitable for preventing and treating intestinal infectious diseases of digestive systems of livestock and poultry.

Instructions for clinical use:

animal experiments show that: the Chinese pulsatilla root granules for livestock and poultry are equivalent to 2g of crude drugs per g, and the using amount is as follows: drinking water or mixing materials: 1-1.5 g of Chinese pulsatilla root particles for livestock and poultry are added into 1L of water of the poultry for 3-5 days continuously. Is suitable for preventing and treating intestinal infectious diseases of digestive systems of livestock and poultry. Has good curative effect on avian colibacillosis, pullorum disease, leucocytozoonosis and other diarrhea caused by unknown reasons, and has quick response and difficult relapse after use.

Application example 1:

and (3) clinical trials: the test is specially carried out for evaluating the clinical curative effect of the Chinese pulsatilla root granules for livestock and poultry on the chicken colibacillosis.

1. Materials and methods

1.1 test drugs

Chinese pulsatilla root granules for livestock and poultry: produced by Henan Narissen biological pharmaceutical industry Co., Ltd according to the preparation process of the invention;

chinese pulsatilla root oral liquid: manufactured according to the national standard by GMP company which is a veterinary drug in Henan.

1.2 test animals

600 commercial Loman laying hens, more than 12000 commercial Loman laying hens of 42 days old are bred by a certain breeding house in Yang, Luo Xiang, Fang suddenly, which is manifested by depressed spirit, loose and disorderly feathers, accumulation of lying abdomen, eye closure, constriction, discharge of white sticky-strip-shaped pasty thin feces, respiratory Ronching, obvious reduction of appetite drinking water, air sac turbidity seen in autopsy, pericardial effusion, hepatomegaly, yellow dyeing, sufficient gallbladder, intestinal bleeding and intestinal wall thickening. Coli can be isolated from the liver of a dead chicken and confirmed as colibacillosis. 500 sick chickens were selected and randomly divided into 5 groups of 100 chickens, and separately bred. In addition, 100 healthy chickens were randomly selected as a control group.

1.3 therapeutic amounts

The above 6 groups were each dosed as in table 1 for 4 days. The chickens were observed for changes in clinical symptoms within two weeks after the start of treatment.

TABLE 1 test grouping and dosing

1.4 evaluation index of therapeutic Effect

The mortality, the efficiency and the cure rate were tested for significance using biometrics, wherein the cure rate was tested using chi-square (X).

2 results

2.1 therapeutic test results

The significant efficiency, cure rate and other conditions of each test group within two weeks after drinking water and taking the medicine are shown in table 2.

TABLE 2 curative effect of Chinese pulsatilla root granules for livestock and poultry on chicken colibacillosis

Group of	Packet situation	Number of animals	Mortality rate	Display efficiency	Cure rate
						1	Healthy control group	100	0	——	——
2	Blank control group	100	68	——	——
						3	High dose group	100	6	94%	92%
4	Middle dose group	100	10	90%	89%
						5	Low dose group	100	28	72%	68%
6	Pulsatilla oral liquid group	100	26	74%	70%

At the end of the test, a small amount of chickens in the medium-dose group, the low-dose group and the drug control group of the Chinese pulsatilla root granules for livestock and poultry have clinical symptoms that excrement adheres to anus.

2.2 statistical analysis

The chi-square (X) test shows that the death rate of each medicine group is extremely obvious and is higher than that of the medicines without infection (P is less than 0.01), and the cure rate is also extremely obvious and is higher than that of the medicines without infection (P is less than 0.01). The animal Chinese pulsatilla root granule high-dose group and the animal Chinese pulsatilla root granule medium-dose group are respectively extremely obvious or remarkably higher than the low-dose group (P is less than 0.01 or 0.01 is less than P is less than 0.05), the animal Chinese pulsatilla root granule high-dose group and the animal Chinese pulsatilla root granule medium-dose group are also extremely obvious in difference (P is less than 0.01) and compared with the animal Chinese pulsatilla root oral liquid medicine control group, and the animal Chinese pulsatilla root granule low-dose group and the animal Chinese pulsatilla root oral liquid medicine control group are not obvious in difference (P is more than 0.05).

Discussion of 3

3.1 the result of statistical analysis of the cure rate shows that the low dose of the Chinese pulsatilla root granules for livestock and poultry has lower curative effect on the chicken colibacillosis. The curative effect of the high and medium dosage is obviously better than that of the low dosage, the cure rate of the low dosage is less than 80 percent, the curative amount is obviously insufficient, and the cure rate of the medium dosage group is obviously higher than that of the low dosage and the medicine group without infection, which shows that the obvious curative effect can be obtained.

3.2 the curative effect between the high and medium dose groups of Chinese pulsatilla root granules for livestock and poultry is not statistically different, but individual chickens have obvious symptoms after the test of the medium dose group is finished, so the high dose is suitable for serious infection, and particularly, when intensive chicken raising groups occur, the feed intake is obviously reduced, and a part of seriously ill chickens are difficult to take enough medicaments in the initial stage, so the proper dosage increase is favorable for achieving the purpose of quick radical cure.

4 evaluation

4.1 the Chinese pulsatilla root granules for livestock and poultry are added with 2L of water per 1g, so that the Chinese pulsatilla root granules are not enough to obtain obvious treatment effect on the chicken colibacillosis.

4.2 the Chinese pulsatilla root granules for livestock and poultry are added with 0.67-1L of water per 1g, so that the Chinese pulsatilla root granules can obtain obvious curative effect on chicken colibacillosis and are superior to a reference drug Chinese pulsatilla root oral liquid.

4.3 according to the test result, the recommended medication scheme is that 0.67-1L of water is added into each 1g of water or 1-1.5 g of the product is added into each L of water, and the medicine is continuously used for 3-5 days.

Application example 2: in vivo bacteriostasis test

1. Strains and culture media

Staphylococcus aureus, Escherichia coli, Salmonella, Pasteurella, Bacillus subtilis, and common agar culture medium.

2. Sample and method

Making radix Pulsatillae granule into series of concentrations of 1.75, 1.5, 1.25, 1, 0.75, 0.5, and 0.25 mg/ml. Adding a proper amount of test bacterial suspension into a plate paved with a bottom layer culture medium by adopting a tube-disc method, placing a small stainless steel tube after solidification, accurately measuring 0.3ml of each sample by using a pipette, adding the sample into each steel tube, and culturing for 24 hours at 37 ℃, wherein the measured results are as follows:

note: the diameter of the inhibition zone is 11-20 mm, and the mark is + to indicate slight sensitivity; 21-30 mm is recorded as ++, which indicates moderate sensitivity; 31-40 mm is recorded as +++, which indicates high sensitivity; greater than 40mm, and is recorded as +++, indicating extreme sensitivity; < 11mm is recorded as-, indicating insensitivity.

Claims

1. The invention relates to a preparation process and application of Chinese pulsatilla root granules for livestock and poultry, wherein the Chinese pulsatilla root granules are prepared from the following medicaments in part by weight: 400-800 g of Chinese pulsatilla root, 150-300 g of coptis root, 450-1000 g of ash bark and 300-600 g of phellodendron; the four components of the Chinese pulsatilla root, the coptis root, the ash bark, the golden cypress and the like are crushed into small pieces for standby, and the Chinese traditional medicine is characterized in that: the method comprises the following steps:

step (1): the method of claim 1, wherein the 4 raw materials are decocted with water for 3 times, 10 times of water is added for 1 hour/time each time, the decoction is combined and filtered, and the filtrate is concentrated to the relative density of 1.20-1.25 (70-80 ℃);

and (4): pulverizing the extract dry extract at below 60 deg.C, adding appropriate amount of two or more adjuvants such as cyclodextrin, starch, silica gel micropowder, lactose, glucose, microcrystalline cellulose, etc., mixing, and granulating with low temperature boiling drying granulator to obtain radix Pulsatillae granule for livestock and fowl; each g is equivalent to 1.3-2.7 g of the original medicine, and the using amount is as follows: drinking water or mixing materials: 1-1.5 g of Chinese pulsatilla root particles for livestock are added into 1L of water of the livestock for 3-5 days, and the Chinese pulsatilla root particles are suitable for preventing and treating intestinal infectious diseases of digestive systems of the livestock.

2. The preparation process and the application of the Chinese pulsatilla root granules for livestock and poultry as claimed in claim 1, the Chinese pulsatilla root granules are prepared from the following medicines by weight: 400g of Chinese pulsatilla root, 150g of coptis root, 450g of ash bark and 300g of phellodendron; the four components of the Chinese pulsatilla root, the coptis root, the ash bark, the golden cypress and the like are crushed into small pieces for standby, and the Chinese traditional medicine is characterized in that: the Chinese pulsatilla root granules for livestock and poultry are obtained according to the steps of the claim 1, each g of the Chinese pulsatilla root granules is equal to 1.3g of the original medicine, and the using amount is as follows: drinking water or mixing materials: 1-1.5 g of Chinese pulsatilla root particles for livestock are added into 1L of water of the livestock for 3-5 days, and the Chinese pulsatilla root particles are suitable for preventing and treating intestinal infectious diseases of digestive systems of the livestock.

3. The preparation process and the application of the Chinese pulsatilla root granules for livestock and poultry as claimed in claim 1, the Chinese pulsatilla root granules are prepared from the following medicines by weight: 800g of Chinese pulsatilla root, 300g of coptis root, 1000g of ash bark and 600g of phellodendron; the four components of the Chinese pulsatilla root, the coptis root, the ash bark, the golden cypress and the like are crushed into small pieces for standby, and the Chinese traditional medicine is characterized in that: the Chinese pulsatilla root granules for livestock and poultry are obtained according to the steps of the claim 1, each g of the Chinese pulsatilla root granules is equivalent to 2.7g of the original medicine, and the using amount is as follows: drinking water or mixing materials: 1-1.5 g of Chinese pulsatilla root particles for livestock are added into 1L of water of the livestock for 3-5 days, and the Chinese pulsatilla root particles are suitable for preventing and treating intestinal infectious diseases of digestive systems of the livestock.

4. The preparation process and the application of the Chinese pulsatilla root granules for livestock and poultry as claimed in claim 1, the Chinese pulsatilla root granules are prepared from the following medicines by weight: 600g of Chinese pulsatilla root, 225g of coptis root, 725g of ash bark and 450g of phellodendron; the four components of the Chinese pulsatilla root, the coptis root, the ash bark, the golden cypress and the like are crushed into small pieces for standby, and the Chinese traditional medicine is characterized in that: the Chinese pulsatilla root granules for livestock and poultry are obtained according to the steps of the claim 1, each g of the Chinese pulsatilla root granules is equivalent to 2g of the original medicine, and the using amount is as follows: drinking water or mixing materials: 1-1.5 g of Chinese pulsatilla root particles for livestock are added into 1L of water of the livestock for 3-5 days, and the Chinese pulsatilla root particles are suitable for preventing and treating intestinal infectious diseases of digestive systems of the livestock.

5. The preparation process and application of the Chinese pulsatilla root granules for livestock and poultry according to claims 1 to 4, characterized in that the relative density of the concentrated extract is 1.20 to 1.25, the temperature of the filtrate during concentration is 70 to 80 ℃, and the dry paste is crushed at the temperature below 60 ℃.

6. The preparation process and application of the Chinese pulsatilla root granules for livestock and poultry as claimed in claim 1, wherein the added auxiliary materials are two or more than two of cyclodextrin, starch, aerosil, lactose, glucose, microcrystalline cellulose and the like.

7. The application of the Chinese pulsatilla root particles for livestock and poultry obtained by the preparation process of the Chinese pulsatilla root particles for livestock and poultry according to claims 1-4 in intestinal infectious diseases of digestive systems of livestock and poultry.

8. The Chinese pulsatilla root granules for livestock and poultry are equivalent to 2g of crude drugs per g, and the using method and the using amount are as follows: drinking water or mixing materials: 1-1.5 g of Chinese pulsatilla root particles for livestock and poultry are added into 1L of water of the poultry for 3-5 days continuously.