CN113820193A - Device and method for treating oral squamous carcinoma in-vitro tumor tissue specimen and application - Google Patents
Device and method for treating oral squamous carcinoma in-vitro tumor tissue specimen and application Download PDFInfo
- Publication number
- CN113820193A CN113820193A CN202110896078.6A CN202110896078A CN113820193A CN 113820193 A CN113820193 A CN 113820193A CN 202110896078 A CN202110896078 A CN 202110896078A CN 113820193 A CN113820193 A CN 113820193A
- Authority
- CN
- China
- Prior art keywords
- groups
- squamous carcinoma
- oral squamous
- tissue specimen
- connecting rods
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 206010028980 Neoplasm Diseases 0.000 title claims abstract description 43
- 206010041823 squamous cell carcinoma Diseases 0.000 title claims abstract description 36
- 238000000338 in vitro Methods 0.000 title claims abstract description 28
- 238000000034 method Methods 0.000 title claims abstract description 23
- 238000005507 spraying Methods 0.000 claims abstract description 17
- 230000001575 pathological effect Effects 0.000 claims abstract description 10
- 210000000214 mouth Anatomy 0.000 claims abstract 2
- 210000001519 tissue Anatomy 0.000 claims description 38
- 239000007921 spray Substances 0.000 claims description 18
- 238000002791 soaking Methods 0.000 claims description 15
- 230000001360 synchronised effect Effects 0.000 claims description 13
- 238000003384 imaging method Methods 0.000 claims description 10
- 239000012452 mother liquor Substances 0.000 claims description 10
- 230000002055 immunohistochemical effect Effects 0.000 claims description 8
- 238000009434 installation Methods 0.000 claims description 7
- 239000008055 phosphate buffer solution Substances 0.000 claims description 7
- WSFSSNUMVMOOMR-UHFFFAOYSA-N Formaldehyde Chemical compound O=C WSFSSNUMVMOOMR-UHFFFAOYSA-N 0.000 claims description 6
- 239000007788 liquid Substances 0.000 claims description 5
- 238000004043 dyeing Methods 0.000 claims description 4
- 238000010603 microCT Methods 0.000 claims description 4
- 238000007865 diluting Methods 0.000 claims description 3
- 210000001165 lymph node Anatomy 0.000 claims description 3
- 238000005406 washing Methods 0.000 claims description 3
- ZCYVEMRRCGMTRW-UHFFFAOYSA-N 7553-56-2 Chemical compound [I] ZCYVEMRRCGMTRW-UHFFFAOYSA-N 0.000 claims description 2
- 229910052740 iodine Inorganic materials 0.000 claims description 2
- 239000011630 iodine Substances 0.000 claims description 2
- 239000000126 substance Substances 0.000 claims description 2
- NJPPVKZQTLUDBO-UHFFFAOYSA-N novaluron Chemical group C1=C(Cl)C(OC(F)(F)C(OC(F)(F)F)F)=CC=C1NC(=O)NC(=O)C1=C(F)C=CC=C1F NJPPVKZQTLUDBO-UHFFFAOYSA-N 0.000 claims 1
- 238000000926 separation method Methods 0.000 claims 1
- 238000007654 immersion Methods 0.000 abstract description 6
- 230000008569 process Effects 0.000 abstract description 5
- 230000008859 change Effects 0.000 abstract description 2
- 208000000102 Squamous Cell Carcinoma of Head and Neck Diseases 0.000 description 7
- 201000002740 oral squamous cell carcinoma Diseases 0.000 description 6
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 4
- 208000035346 Margins of Excision Diseases 0.000 description 3
- 230000009471 action Effects 0.000 description 3
- 239000007853 buffer solution Substances 0.000 description 3
- 239000000243 solution Substances 0.000 description 3
- 238000003860 storage Methods 0.000 description 3
- 230000007306 turnover Effects 0.000 description 3
- 201000011510 cancer Diseases 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 238000011156 evaluation Methods 0.000 description 2
- 229910052757 nitrogen Inorganic materials 0.000 description 2
- 201000009030 Carcinoma Diseases 0.000 description 1
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 1
- 238000003491 array Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000005540 biological transmission Effects 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 239000003086 colorant Substances 0.000 description 1
- 238000005138 cryopreservation Methods 0.000 description 1
- 238000003745 diagnosis Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000007710 freezing Methods 0.000 description 1
- 230000008014 freezing Effects 0.000 description 1
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 description 1
- 201000010536 head and neck cancer Diseases 0.000 description 1
- 238000007689 inspection Methods 0.000 description 1
- 238000010030 laminating Methods 0.000 description 1
- 230000003902 lesion Effects 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 230000001338 necrotic effect Effects 0.000 description 1
- 208000010655 oral cavity squamous cell carcinoma Diseases 0.000 description 1
- 230000010355 oscillation Effects 0.000 description 1
- 230000007170 pathology Effects 0.000 description 1
- 230000002093 peripheral effect Effects 0.000 description 1
- 230000002980 postoperative effect Effects 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 238000011127 radiochemotherapy Methods 0.000 description 1
- 238000005096 rolling process Methods 0.000 description 1
- 238000005070 sampling Methods 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 230000004083 survival effect Effects 0.000 description 1
Images
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
- G01N1/31—Apparatus therefor
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2/00—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor
- A61L2/02—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor using physical phenomena
- A61L2/04—Heat
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/2806—Means for preparing replicas of specimens, e.g. for microscopal analysis
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/34—Purifying; Cleaning
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/42—Low-temperature sample treatment, e.g. cryofixation
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N23/00—Investigating or analysing materials by the use of wave or particle radiation, e.g. X-rays or neutrons, not covered by groups G01N3/00 – G01N17/00, G01N21/00 or G01N22/00
- G01N23/02—Investigating or analysing materials by the use of wave or particle radiation, e.g. X-rays or neutrons, not covered by groups G01N3/00 – G01N17/00, G01N21/00 or G01N22/00 by transmitting the radiation through the material
- G01N23/04—Investigating or analysing materials by the use of wave or particle radiation, e.g. X-rays or neutrons, not covered by groups G01N3/00 – G01N17/00, G01N21/00 or G01N22/00 by transmitting the radiation through the material and forming images of the material
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Immunology (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- Chemical & Material Sciences (AREA)
- General Physics & Mathematics (AREA)
- Physics & Mathematics (AREA)
- Pathology (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Epidemiology (AREA)
- Animal Behavior & Ethology (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
The invention discloses an oral squamous carcinoma in-vitro tumor tissue specimen processing device which comprises a bearing component, a turning component, a spraying component and an immersion box, wherein the bearing component is rotatably arranged in the immersion box through the turning component and is close to the box opening of the immersion box; bearing assembly includes alternately supporting rod, bearing card box and drive assembly, bearing card box includes two sets of hollow casings that are the hemisphere setting, two sets of hollow casings symmetry respectively set up in alternately supporting rod top, and the interior annular equidistance array of hollow casing has the aperture, this device simple structure, occupation space is little, not only be applicable to the laboratory, still directly be used for clinically, there is not the limitation, alleviate operator's work load, once support oral cavity squamous carcinoma in vitro sample, it soaks and abluent position need not to change, reduce the influence that external factor brought for the in vitro sample, operation process is simple quick, the cost is low, do not influence follow-up pathological examination.
Description
Technical Field
The invention relates to the technical field of oral squamous cell carcinoma, in particular to a device and a method for treating an oral squamous cell carcinoma in-vitro tumor tissue specimen and application thereof.
Background
Squamous cell carcinoma of the mouth is one of the common malignant tumors of the head and neck. The data show that the 5-year survival rate is about 50%, the late-stage patients are less than 30%, and the relapsers are less than 20%. The existing treatment method of oral squamous cell carcinoma is comprehensive sequence treatment mainly based on operation and assisted by means of chemoradiotherapy and the like. Studies have shown that local and regional recurrence is one of the major causes of failure in treatment of oral squamous cell carcinoma. Among them, complete and complete removal of tumor lesions (negative surgical margin) is the key to avoid local recurrence of oral squamous cell carcinoma.
The main means for evaluating the incisal margin of oral squamous cell carcinoma operation at present are: (1) the possibility of misdiagnosis and missed diagnosis exists through the subjective judgment of imaging and clinical examination before operation; (2) in the operation, the in-vitro tumor focus and the tumor bed operation wound periphery are sampled to carry out in-operation quick frozen slice inspection, so that the possibility of sampling errors exists; (3) post-operative routine pathology examination assessment of surgical margin distance (whether surgical margin on pathological section is >0.5cm from tumor) is the current gold standard. Then, the technology is only two-dimensional evaluation, and cannot carry out integral evaluation on a three-dimensional layer; (4) full sample serial section examination.
At present, the most reliable method for three-dimensionally evaluating the state of the oral squamous carcinoma in-vitro specimen is used, but the workload is too large, the method can only be applied to laboratories, and the possibility of clinical practice is not available. Therefore, it is very important to find a new method for evaluating the state of the oral squamous carcinoma in vitro tumor specimen at a three-dimensional level.
Disclosure of Invention
The invention aims to provide a device and a method for treating an oral squamous carcinoma in-vitro tumor tissue specimen and application thereof, which have small occupied space, are not only suitable for laboratories, but also can be directly used for clinics without limitation, and reduce the workload of operators so as to solve the problems in the background art.
In order to achieve the purpose, the invention provides the following technical scheme:
an oral squamous carcinoma in-vitro tumor tissue specimen processing device comprises a bearing component, a turning component, a spraying component and an immersion box, wherein the bearing component is rotatably arranged in the immersion box through the turning component and is close to the box opening of the immersion box;
the bearing component comprises cross clamping rods, a bearing card box and a driving component, the bearing card box comprises two groups of semi-spherical hollow shells, the two groups of hollow shells are respectively and symmetrically arranged at the tops of the cross clamping rods, and small holes are formed in the hollow shells in an annular equidistant array;
the crossed clamping rods comprise two groups of first connecting rods, two groups of second connecting rods and two groups of third connecting rods, one ends of the first connecting rods are respectively connected with a first connecting block and a second connecting block in a pin mode, the two groups of first connecting rods are connected with the middle position in a crossed pin mode, one ends of the two groups of first connecting rods, far away from the first connecting block and the second connecting block, are respectively connected with the two groups of second connecting rods in a pin mode to rotate, the two groups of third connecting rods are respectively connected with the two groups of second connecting rods in a pin mode to rotate, one ends of the two groups of second connecting rods, far away from the first connecting rods, and the two groups of hollow shells are respectively and fixedly installed at one ends of the two groups of third connecting rods. .
At least 8 groups of limit teeth are arranged at the pin joint position of the second connecting rod and the first connecting rod in an array mode, and at least 8 groups of limit teeth are also arranged at the pin joint position of the third connecting rod and the second connecting rod.
The driving assembly comprises an installation groove and a screw rod, the screw rod is rotatably installed in the installation groove, the first connecting block is in threaded connection with the screw rod, and the second connecting block is fixedly installed on one side of the outer wall of the installation groove;
wherein, upset subassembly includes revolving cylinder and rotation axis, rotation axis one end fixed mounting is in mounting groove one side, and the rotation axis other end rotates through rolling bearing and soak the incasement wall and connect, revolving rotary table fixed mounting of revolving cylinder is in mounting groove one side.
The spraying assembly comprises a spraying cavity and a spraying pipe arranged at the bottom of the spraying cavity in an annular array mode, the spraying pipe is fixedly communicated with the spraying cavity, the spraying cavity is fixedly communicated with an external pump body through a pipeline, a high-pressure nozzle is fixedly communicated with a liquid outlet of the spraying pipe, and the bearing card box is arranged under the four groups of high-pressure nozzles.
The mounting groove is fixedly provided with a motor, the output shaft end of the motor is fixedly provided with a synchronous belt pulley, one end of the screw rod is also fixedly provided with a synchronous belt pulley, and the synchronous belt pulleys are connected with one another through a synchronous belt.
The first connecting block comprises a U-shaped lug seat and a supporting block fixedly installed at the bottom of the U-shaped lug seat, the surface of the screw rod is in threaded connection with a threaded sleeve, the supporting block and the threaded sleeve are rotatably connected, the second connecting block also comprises a U-shaped lug seat, a supporting plate is fixedly connected to the bottom of the U-shaped lug seat of the second connecting block, and the supporting plate is fixedly installed on one side of the outer wall of the installation groove.
The use method of the oral squamous carcinoma in-vitro tumor tissue specimen processing device comprises the following steps:
s1: obtaining oral squamous carcinoma in-vitro tumor tissue specimens (primary focus specimens and lymph node specimens), placing the oral squamous carcinoma in a bearing card box, and placing the oral squamous carcinoma in the bearing card box;
s2: diluting Lugol's iododine mother liquor to 3% concentration by using formalin, filling the 3% Lugol's iododine mother liquor into a soaking box, starting a rotary cylinder, soaking an oral squamous carcinoma in-vitro tumor tissue specimen in a bearing cassette into the 3% Lugol's iododine mother liquor, and oscillating for 1-2 hours by using ultrasonic waves;
s3: after the dip dyeing, starting the rotary air cylinder, turning over the specimen in the bearing card box to be close to the lower part of the high-pressure spray head, and washing the specimen for 1 minute by using PBS (phosphate buffer solution) which is pre-filled in the spray cavity;
s4: after the processing is finished, scanning and imaging are carried out by using micro-CT imaging equipment, CT image data are obtained, and the CT data are analyzed conventionally;
s5: tumor specimens were subjected to routine pathological section examination and immunohistochemical examination.
An application of the treated in vitro tumor tissue specimen of oral squamous carcinoma in the conventional pathological section examination and immunohistochemical examination is disclosed.
In summary, due to the adoption of the technology, the invention has the beneficial effects that:
the invention provides an integrated device, which is sequentially provided with a soaking box, a supporting component and a turning component, wherein after oral squamous carcinoma in-vitro tumor tissues are taken out, the oral squamous carcinoma in-vitro tumor tissues are placed in supporting card boxes, a screw rod is started to enable the two groups of supporting card boxes to be seamlessly attached, the supporting card boxes are directly turned over, the supporting card boxes are immersed in the soaking box filled with Lugol's iododine mother liquor with the concentration of 3%, after ultrasonic oscillation treatment, the supporting card boxes are turned over to be under a spraying device, a high-pressure spray head is utilized to wash the supporting card boxes with PBS buffer solution, and finally micro-CT imaging equipment (with the resolution of 25 mu m) is directly used for scanning imaging to obtain CT image data, the CT data is routinely analyzed, and subsequent routine pathological section examination and immunohistochemical examination can be carried out.
The device has a simple structure and small occupied space, is suitable for laboratories, can be directly used for clinics, has no limitation, reduces the workload of an operator, supports the oral squamous carcinoma in-vitro specimen at one time, does not need to change the soaking and cleaning positions of the oral squamous carcinoma in-vitro specimen, and reduces the influence of external factors on the in-vitro specimen.
Drawings
FIG. 1 is a schematic perspective view of the present invention;
FIG. 2 is a schematic front view of the present invention;
FIG. 3 is a schematic top view of the present invention;
FIG. 4 is a side cross-sectional structural view of the first connecting block of the present invention;
FIG. 5 is a schematic view of a limiting tooth structure according to the present invention;
FIG. 6 is a flow chart of a method of use in the present invention.
In the figure: 1. a holding assembly; 2. a turnover assembly; 201. a rotating cylinder; 202. a rotating shaft; 3. a spray assembly; 301. a spray chamber; 302. a shower pipe; 303. a high pressure spray head; 4. a soaking box; 5. crossed clamping rods; 501. a first link; 502. a second link; 503. a third link; 504. a first connection block; 5041. a U-shaped ear seat; 5042. a support block; 5043. a threaded sleeve; 5044. a support plate; 505. a second connecting block; 506. limiting teeth; 6. supporting the card box; 601. a hollow shell; 602. a small hole; 7. a drive assembly; 701. mounting grooves; 702. a screw rod; 703. an electric motor.
Detailed Description
In order to make the objects, technical solutions and advantages of the embodiments of the present invention more apparent, the technical solutions of the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings of the embodiments of the present invention, and it is obvious that the described embodiments are some, but not all embodiments of the present invention. All other embodiments, which can be obtained by a person skilled in the art without any inventive step based on the embodiments of the present invention, are within the scope of the present invention. Thus, the following detailed description of the embodiments of the present invention, presented in the figures, is not intended to limit the scope of the invention, as claimed, but is merely representative of selected embodiments of the invention. All other embodiments, which can be obtained by a person skilled in the art without any inventive step based on the embodiments of the present invention, are within the scope of the present invention.
In the description of the present invention, it is to be understood that the terms indicating an orientation or positional relationship are based on the orientation or positional relationship shown in the drawings only for the convenience of describing the present invention and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and thus, should not be construed as limiting the present invention.
In the present invention, unless otherwise expressly stated or limited, the terms "mounted," "connected," "secured," and the like are to be construed broadly and can, for example, be fixedly connected, detachably connected, or integrally formed; can be mechanically or electrically connected; either directly or indirectly through intervening media, either internally or in any other relationship. The specific meaning of the above terms in the present invention can be understood by those of ordinary skill in the art from the specification.
The invention provides an oral squamous carcinoma in-vitro tumor tissue specimen processing device shown in figures 1-5, which comprises a bearing component 1, a turnover component 2, a spraying component 3 and a soaking box 4, and is characterized in that: bearing subassembly 1 rotates through upset subassembly 2 and installs in soaking case 4 and bearing subassembly 1 is close to the case mouth department that soaks case 4, 3 fixed mounting in bearing subassembly top of spray assembly, spray assembly 3 is including spraying chamber 301 and annular array in the shower 302 that sprays the chamber 301 bottom, shower 302 with spray the fixed intercommunication in chamber 301, spray chamber 301 and the fixed intercommunication of outside pump body, the fixed intercommunication of liquid outlet of shower 302 has high pressure nozzle 303, bearing card box 6 sets up under four high pressure nozzle 303 of group, has filled PBS buffer solution in spraying chamber 301 in advance, soaks the back to the sample in bearing card box 6, directly starts the pump body and utilizes high pressure nozzle 303 to wash it, and convenient and fast need not to take out conversion work space, reduces operator's work load.
The bearing component 1 comprises cross clamping rods 5, a bearing card box 6 and a driving component 7, the bearing card box 6 comprises two groups of hollow shells 601 arranged in a hemispherical manner, the two groups of hollow shells 601 are respectively and symmetrically arranged at the tops of the cross clamping rods 5, and annular equidistant arrays in the hollow shells 601 are provided with small holes 602 which are provided with the small holes 602, so that a specimen can be wrapped by the hollow shells 601 without affecting the soaking and washing effects, the bearing card box 6 can be more experienced by technicians in the field to be arranged in specifications, and the bearing card box can be connected with the third connecting rod 503 through a threaded rod, so that the bearing card box is convenient to disassemble and replace;
the crossed clamping rod 5 comprises two groups of first connecting rods 501, two groups of second connecting rods 502 and two groups of third connecting rods 503, one end of each of the two groups of first connecting rods 501 is respectively in pin joint with a first connecting block 504 and a second connecting block 505, the two groups of first connecting rods 501 are in cross pin joint with the central position, one ends of the two groups of first connecting rods 501, which are far away from the first connecting block 504 and the second connecting block 505, are respectively in pin joint with the two groups of second connecting rods 502 to rotate, the two groups of third connecting rods 503 are respectively in pin joint with the ends of the two groups of second connecting rods 502, the two groups of hollow shells 601 are respectively fixedly installed at one ends of the two groups of third connecting rods 503, the pin joint positions of the second connecting rods 502 and the first connecting rods 501 are arrayed with at least 8 groups of limiting teeth 506, the pin joint positions of the third connecting rods 503 and the second connecting rods 502 are also provided with at least 8 groups of limiting teeth 506, and the limiting teeth 506 are arranged to limit the space angles of the second connecting rods 502 and the third connecting rods 503, and utilize spacing tooth 506 spacing, at the in-process that two sets of first connecting rods 501 merged, the process of the seamless laminating of two sets of hollow shells 601 of cooperation can not receive the influence and interfere, when preventing the sample, the manual work sets up hollow upwards slope of hollow shell 601, along with the drive of lead screw 702, two sets of first connecting rods 501 contained angles diminish, and two sets of hollow shells 601 slowly are close to until seamless cohesion.
The driving assembly 7 comprises a mounting groove 701 and a lead screw 702, the lead screw 702 is rotatably mounted in the mounting groove 701, the first connecting block 504 is connected with the screw rod 702 in a threaded manner, the second connecting block 505 is fixedly arranged at one side of the outer wall of the mounting groove 701, the turnover assembly 2 comprises a rotary cylinder 201 and a rotary shaft 202, one end of the rotary shaft 202 is fixedly arranged at one side of the mounting groove 701, and the other end of the rotating shaft 202 is rotatably connected with the inner wall of the soaking box 4 through a bearing with a seat, the rotating turntable of the rotating cylinder 201 is fixedly arranged at one side of the mounting groove 701, a motor 703 is fixedly arranged on the outer wall of the mounting groove 701, a synchronous belt wheel is fixedly arranged on an output shaft end of the motor 703, one end of the screw rod 702 is also fixedly provided with synchronous belt wheels, two groups of synchronous belt wheels are connected through synchronous belt transmission, and the screw rod 702 can be driven to rotate by the motor 703 and the synchronous belt wheels, so that the two groups of first connecting rods 501 are driven to open and close.
The first connecting block 504 includes U type ear seat 5041 and fixed mounting in the supporting shoe 5042 of U type ear seat 5041 bottom, lead screw 702 surface threaded connection has swivel nut 5043, supporting shoe 5042 and swivel nut 5043 rotate the connection, the second connecting block 505 also includes U type ear seat 5041, the U type ear seat 5041 bottom fixed connection of second connecting block 505 has a supporting plate 5044, supporting plate 5044 fixed mounting is in mounting groove 701 outer wall one side, and this section describes first connecting block 504 and the concrete structure setting of lead screw 702 threaded connection.
The invention also provides a use method of the oral squamous carcinoma in-vitro tumor tissue specimen processing device shown in figure 6, which comprises the following steps:
s1: obtaining oral squamous carcinoma in-vitro tumor tissue specimens (primary focus specimens and lymph node specimens), and placing the specimens into a bearing card box 6;
s2: diluting Lugol's iododine mother liquor to 3% iodine simple substance concentration by using formalin, pouring the Lugol's iododine mother liquor with the concentration of 3% into the soaking box 4, starting the rotary cylinder 201, dip-dyeing the oral squamous carcinoma in-vitro tumor tissue specimen in the bearing box 6 into the Lugol's iododine mother liquor with the concentration of 3%, and oscillating for 1-2 hours by using ultrasonic;
s3: after the dip dyeing, the rotary cylinder 201 is started again, the sample in the bearing card box 6 is turned to be close to the lower part of the high-pressure spray nozzle 303, and the sample is washed for 1 minute by using PBS buffer solution which is pre-filled in the spray cavity 301;
s4: after the processing is finished, scanning and imaging are carried out by micro-CT imaging equipment (with the resolution of 25um), CT image data are obtained, and the CT data are analyzed conventionally;
s5: tumor specimens were subjected to routine pathological section examination and immunohistochemical examination.
The invention also provides application of the treated oral squamous carcinoma in vitro tumor tissue specimen in conventional pathological section examination and immunohistochemical examination.
The collection procedure for common tumors included: preparation work: the main equipment comprises 2 scissors, 2 tweezers, a plurality of blades, normal saline, freezing tubes with different colors, a temporary register book, a marking pen, label paper, liquid nitrogen and a portable specimen storage tank; -86 ℃ deep low temperature freezer; sterilizing the surgical instruments and the storage tube at high temperature and high pressure; specimen selection: the selected specimen comprises tumor tissue, normal tissue and/or cancer adjacent tissue; method and timing for specimen excision: firstly, observing or photographing a general specimen, confirming the range of a tumor part, paying attention to the identification of peripheral tissues and necrotic tissues, incising the specimen in the shortest time (<30min) after the specimen is separated from a body, incising tissues beside cancer and carcinoma into a plurality of tissue blocks with the diameter of about 0.5cm, respectively filling the tissue blocks into numbered sterilized cryopreservation tubes, quickly putting the tube into a liquid nitrogen transfer tank and preparing for long-term storage. According to the invention, after being taken out, the preserved tissue specimen is directly placed into the bearing card box 6, and after being processed, the tissue specimen is directly entered into the three-dimensional high-resolution imaging process of the cavity squamous carcinoma in vitro tissue, CT image data is obtained, the CT data is analyzed conventionally, and conventional pathological section examination and immunohistochemical examination are carried out on the tumor specimen.
The above description is only for the preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art should be considered to be within the technical scope of the present invention, and the technical solutions and the inventive concepts thereof according to the present invention should be equivalent or changed within the scope of the present invention.
It is noted that, herein, relational terms such as first and second, and the like may be used solely to distinguish one entity or action from another entity or action without necessarily requiring or implying any actual such relationship or order between such entities or actions. Also, the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or apparatus.
Claims (10)
1. The utility model provides an oral cavity squamous carcinoma separation tumour tissue specimen processing apparatus, includes bearing subassembly (1), upset subassembly (2) and spray set spare (3) and soaks case (4), its characterized in that: the supporting component (1) is rotatably arranged in the soaking box (4) through the overturning component (2), the supporting component (1) is close to the box opening of the soaking box (4), and the spraying component (3) is fixedly arranged above the supporting component (1);
bearing subassembly (1) is including crossing supporting rod (5), bearing card box (6) and drive assembly (7), bearing card box (6) are two sets of hollow casing (601) that are the hemisphere setting including, and two sets of hollow casing (601) symmetry respectively set up in crossing supporting rod (5) top, and the equidistant array of annular has aperture (602) in hollow casing (601).
2. The device for treating the tissue specimen of the oral squamous carcinoma isolated tumor according to claim 1, wherein: the crossed clamping rod (5) comprises two groups of first connecting rods (501), two groups of second connecting rods (502) and two groups of third connecting rods (503), one ends of the two groups of first connecting rods (501) are respectively in pin joint with a first connecting block (504) and a second connecting block (505), the two groups of first connecting rods (501) are in cross pin joint with the middle position, one ends of the two groups of first connecting rods (501) far away from the first connecting block (504) and the second connecting block (505) are respectively in pin joint rotation with the two groups of second connecting rods (502), the two groups of third connecting rods (503) are respectively in pin joint rotation with one ends of the two groups of second connecting rods (502) far away from the first connecting rods (501), and the two groups of hollow shells (601) are respectively and fixedly installed at one ends of the two groups of third connecting rods (503).
3. The device for processing the tissue specimen of the oral squamous carcinoma isolated tumor according to claim 2, wherein: at least 8 groups of limit teeth (506) are arrayed at the pin joint position of the second connecting rod (502) and the first connecting rod (501), and at least 8 groups of limit teeth (506) are also arranged at the pin joint position of the third connecting rod (503) and the second connecting rod (502).
4. The device for treating the tissue specimen of the oral squamous carcinoma isolated tumor according to claim 1, wherein: the driving assembly (7) comprises an installation groove (701) and a screw rod (702), the screw rod (702) is rotatably installed in the installation groove (701), the first connecting block (504) is in threaded connection with the screw rod (702), and the second connecting block (505) is fixedly installed on one side of the outer wall of the installation groove (701).
5. The device for treating the tissue specimen of the oral squamous carcinoma isolated tumor according to claim 4, wherein: upset subassembly (2) include revolving cylinder (201) and rotation axis (202), rotation axis (202) one end fixed mounting is in mounting groove (701) one side, and rotation axis (202) other end passes through the pedestal bearing and soaks case (4) inner wall and rotate the connection, the rotating carousel fixed mounting of revolving cylinder (201) is in mounting groove (701) one side.
6. The device for treating the tissue specimen of the oral squamous carcinoma isolated tumor according to claim 1, wherein: spray assembly (3) are including spraying chamber (301) and annular array in shower (302) that spray chamber (301) bottom, shower (302) and the fixed intercommunication in shower (301), it communicates with the outside pump body is fixed to spray chamber (301) through the pipeline, the fixed intercommunication of liquid outlet of shower (302) has high pressure nozzle (303), bearing card box (6) set up under four groups high pressure nozzle (303).
7. The device for treating the tissue specimen of the oral squamous carcinoma isolated tumor according to claim 4, wherein: the outer wall of the mounting groove (701) is fixedly provided with a motor (703), an output shaft end of the motor (703) is fixedly provided with a synchronous belt wheel, one end of the screw rod (702) is also fixedly provided with a synchronous belt wheel, and the two groups of synchronous belt wheels are connected through a synchronous belt.
8. The device for processing the tissue specimen of the oral squamous carcinoma isolated tumor according to claim 2, wherein: first connecting block (504) include U type ear seat (5041) and fixed mounting in supporting shoe (5042) of U type ear seat (5041) bottom, lead screw (702) surface thread is connected with swivel nut (5043), supporting shoe (5042) and swivel nut (5043) rotate to be connected, second connecting block (505) also include U type ear seat (5041), U type ear seat (5041) bottom fixedly connected with backup pad (5044) of second connecting block (505), backup pad (5044) fixed mounting is in mounting groove (701) outer wall one side.
9. The use method of the oral squamous carcinoma ex-vivo tumor tissue specimen processing device according to claim 1, which comprises the following steps:
s1: obtaining oral squamous carcinoma in-vitro tumor tissue specimens (primary focus specimens and lymph node specimens), and placing the specimens into a bearing card box (6);
s2: diluting Lugol's iododine mother liquor to 3% concentration (iodine simple substance concentration) by using formalin, pouring the Lugol's iododine mother liquor with the 3% concentration into a soaking box (4), starting a rotary cylinder (201), soaking an oral squamous carcinoma in-vitro tumor tissue specimen in a bearing box (6) into the Lugol's iododine mother liquor with the concentration of 3%, and oscillating for 1-2 hours by using ultrasonic;
s3: after the dip dyeing, starting the rotary cylinder (201), turning the sample in the bearing card box (6) to be close to the lower part of the high-pressure spray nozzle (303), and washing the sample for 1 minute by using PBS (phosphate buffer solution) pre-filled in the spraying cavity (301);
s4: after the processing is finished, scanning and imaging are carried out by micro-CT imaging equipment (with the resolution of 25um), CT image data are obtained, and the CT data are analyzed conventionally;
s5: tumor specimens were subjected to routine pathological section examination and immunohistochemical examination.
10. Use of an ex vivo tumor tissue specimen of oral squamous carcinoma treated according to the method of any of claims 1-9 in routine pathological section examination and immunohistochemical examination.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110896078.6A CN113820193A (en) | 2021-08-05 | 2021-08-05 | Device and method for treating oral squamous carcinoma in-vitro tumor tissue specimen and application |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110896078.6A CN113820193A (en) | 2021-08-05 | 2021-08-05 | Device and method for treating oral squamous carcinoma in-vitro tumor tissue specimen and application |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN113820193A true CN113820193A (en) | 2021-12-21 |
Family
ID=78912861
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202110896078.6A Pending CN113820193A (en) | 2021-08-05 | 2021-08-05 | Device and method for treating oral squamous carcinoma in-vitro tumor tissue specimen and application |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN113820193A (en) |
Citations (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107346753A (en) * | 2016-05-06 | 2017-11-14 | 亿力鑫系统科技股份有限公司 | soaking device |
| CN107824532A (en) * | 2017-10-30 | 2018-03-23 | 陈永 | A kind of machine components Rapid Cleaning air-dry apparatus |
| CN107962030A (en) * | 2018-01-15 | 2018-04-27 | 贵阳锐航智能科技有限公司 | A kind of Rapid Cleaning drying unit for medical instrument |
| CN207858835U (en) * | 2017-11-28 | 2018-09-14 | 天津泰沃纳科技有限公司 | A kind of industrial robot grabbing device |
| CN109387419A (en) * | 2018-12-05 | 2019-02-26 | 滨州医学院 | A kind of dyeing of microsection manufacture and scanning integrated machine |
| CN210670794U (en) * | 2019-09-24 | 2020-06-02 | 深圳市松柏实业发展有限公司 | Soaking mechanism of surface treatment system |
| CN111842323A (en) * | 2020-07-21 | 2020-10-30 | 恒超源洗净科技(深圳)有限公司 | Integrated ultrasonic part cleaning device |
| CN212254805U (en) * | 2020-06-11 | 2020-12-29 | 徐正军 | Lung cancer screening kit |
| CN112621797A (en) * | 2020-12-01 | 2021-04-09 | 镇江云杰信息技术有限公司 | Clamping arm for industrial robot |
| CN213406422U (en) * | 2020-08-20 | 2021-06-11 | 赵婧 | Fixing device is handled to medical science experiment mouse |
| CN213570199U (en) * | 2020-11-20 | 2021-06-29 | 滁州明永光电科技有限公司 | Soak device for camera glass lens |
-
2021
- 2021-08-05 CN CN202110896078.6A patent/CN113820193A/en active Pending
Patent Citations (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107346753A (en) * | 2016-05-06 | 2017-11-14 | 亿力鑫系统科技股份有限公司 | soaking device |
| CN107824532A (en) * | 2017-10-30 | 2018-03-23 | 陈永 | A kind of machine components Rapid Cleaning air-dry apparatus |
| CN207858835U (en) * | 2017-11-28 | 2018-09-14 | 天津泰沃纳科技有限公司 | A kind of industrial robot grabbing device |
| CN107962030A (en) * | 2018-01-15 | 2018-04-27 | 贵阳锐航智能科技有限公司 | A kind of Rapid Cleaning drying unit for medical instrument |
| CN109387419A (en) * | 2018-12-05 | 2019-02-26 | 滨州医学院 | A kind of dyeing of microsection manufacture and scanning integrated machine |
| CN210670794U (en) * | 2019-09-24 | 2020-06-02 | 深圳市松柏实业发展有限公司 | Soaking mechanism of surface treatment system |
| CN212254805U (en) * | 2020-06-11 | 2020-12-29 | 徐正军 | Lung cancer screening kit |
| CN111842323A (en) * | 2020-07-21 | 2020-10-30 | 恒超源洗净科技(深圳)有限公司 | Integrated ultrasonic part cleaning device |
| CN213406422U (en) * | 2020-08-20 | 2021-06-11 | 赵婧 | Fixing device is handled to medical science experiment mouse |
| CN213570199U (en) * | 2020-11-20 | 2021-06-29 | 滁州明永光电科技有限公司 | Soak device for camera glass lens |
| CN112621797A (en) * | 2020-12-01 | 2021-04-09 | 镇江云杰信息技术有限公司 | Clamping arm for industrial robot |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US3881464A (en) | Sampling device and method | |
| CN107692975A (en) | Three-dimensional optoacoustic laminated imaging device and method | |
| CN113820193A (en) | Device and method for treating oral squamous carcinoma in-vitro tumor tissue specimen and application | |
| CN206787852U (en) | A kind of internal medicine assay device | |
| CN209064600U (en) | A kind of medical Pathologic specimen bottle storage device | |
| CN210045675U (en) | Rotation type ophthalmology apparatus wash rack | |
| CN207764245U (en) | A kind of pharmaceutical test room sample batch verifying attachment | |
| CN209680758U (en) | A kind of test tubes inside and outside wall cleaning device | |
| CN111671471A (en) | Urine sample stays gets device convenient to stay and take and carry out anticorrosive treatment | |
| CN213787505U (en) | Ultrasonic diagnostic apparatus convenient to use | |
| CN110118765A (en) | A kind of preparation of SERS substrate and its application in terms of cancer detection | |
| CN217069886U (en) | Probe disinfecting equipment based on ultrasonic department | |
| CN207561920U (en) | Moveable desk-top optoacoustic and ultrasonic synchronous imaging device | |
| CN214632193U (en) | Whole machine waterproof wireless probe type ultrasonic diagnostic instrument capable of achieving quick sterile disinfection | |
| CN108580464A (en) | A kind of clinical laboratory's cleaning device | |
| CN211327092U (en) | Medical degassing unit for probe | |
| CN211121278U (en) | Clinical examination device | |
| CN209332114U (en) | Spiral quantitative skin trephine biopsy device tool | |
| CN215965360U (en) | Urine sampling test tube cleaning device for urological department | |
| CN208313669U (en) | A kind of novel dropsy of serous cavity subpackage apparatus | |
| CN201909722U (en) | Device for sampling and flushing pathological samples | |
| CN219301189U (en) | Sample batch quick drying device for medical examination | |
| CN216622402U (en) | Sealed blood test bench | |
| CN208554309U (en) | Rack for test tube is used in a kind of detection of medicine | |
| CN213842807U (en) | Pathology department's sample preprocessing device |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination |