CN113817646A - Simple photosynthetic bacteria culture medium and application thereof - Google Patents

Simple photosynthetic bacteria culture medium and application thereof Download PDF

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CN113817646A
CN113817646A CN202111187457.4A CN202111187457A CN113817646A CN 113817646 A CN113817646 A CN 113817646A CN 202111187457 A CN202111187457 A CN 202111187457A CN 113817646 A CN113817646 A CN 113817646A
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photosynthetic bacteria
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巩华
赖迎迢
陈总会
孙承文
赵长臣
江小燕
刘春花
陶家发
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Pearl River Fisheries Research Institute CAFS
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Abstract

The invention belongs to the technical field of culture of photosynthetic bacteria, and particularly relates to a simple photosynthetic bacteria culture medium and application thereof3、VB12And VB1The culture medium has simple and practical formula and high production efficiency, has no special requirements on the grade of the used medicine, can reach the agricultural fertilizer grade, has easily obtained, cheap and stable raw materials, has the same enrichment effect on rhodopseudomonas palustris, rhodobacter capsulatus, rhodospirillum rubrum and other photosynthetic bacteria in the rhodospirillaceae family within the range of suitable conditions, and can reach the viable count of 1 multiplied by 10 in 3 days9The method is suitable for large-scale production, is applied to culture enrichment and separation of photosynthetic bacteria, can accelerate culture, improves production efficiency, and has good effectTheoretical value and practical production significance.

Description

Simple photosynthetic bacteria culture medium and application thereof
Technical Field
The invention belongs to the technical field of culture of photosynthetic bacteria, and particularly relates to a simple photosynthetic bacteria culture medium and application thereof.
Background
Photosynthetic Bacteria (PSB) are a generic term for a class of prokaryotes that have an original Photosynthetic system and are capable of photosynthesis in anaerobic or facultative anaerobic environments without the production of oxygen. The photosynthetic bacteria belong to Rhodospirillales, Rhodospirillum rubrum, Rhodospirillum calophyllum, Rhodospirillum megaterium, Rhodospirillum microsclerum, Rhodospirillum gracilis, Rhodospirillum brownii, etc. The bacteria all grow in water and sludge, belong to anaerobic or microaerophilic bacteria, and synthesize organic matters by utilizing light energy under anaerobic conditions. The cells of the bacteria are coiled in a spiral shape, have different lengths and unequal number of twists, and the cells of the same kind have large and variable sizes, are in end-to-end cluster and can move. The cells contain bacteriopurpurin, bacteriochlorin, carotenoid and the like, so the bacteria are in red, brownish red, crimson or rose color. Among them, rhodopseudomonas palustris is columnar, occasionally has bent, encapsulated and polar flagellum, can move, and cell masses are in red or purple with different depths, are the most common photosynthetic bacteria, are representative species of photosynthetic bacteria which do not produce oxygen, and are widely distributed in nature, especially in aquatic environment with high concentration of putrefactive organic substances. Meanwhile, the rhodopseudomonas palustris is one of feed-grade microorganisms allowed to be used in the early stage of China, has nitrogen metabolism mechanisms such as nitrogen fixation, ammoniation, ammonia assimilation and denitrification and is widely applied to aquaculture production. The photosynthetic bacteria have strong vitality, quick propagation and low nutritional requirement on the growth; the photosynthetic bacteria are rich in carotenoid, protein, vitamin B and coenzyme Q10, have the characteristics of non-toxic action and the like, play more and more important roles in many fields and many aspects of human life, and particularly play a role in the fields of organic wastewater treatment, biohydrogen production, biological pharmacy and the like. In recent years, the optimization of the culture method of photosynthetic bacteria, especially the research of the culture technology of high-concentration bacteria liquid, has been widely regarded. However, the biological application of photosynthetic bacteria is severely restricted due to the culture technology of high-concentration photosynthetic bacteria liquid and the lag of the simple detection, judgment and evaluation method and standard establishment of colony count.
At present, the best process for producing microorganisms such as photosynthetic bacteria and the like is liquid fermentation, but some enterprises adopt a solid fermentation method with low investment and simple process to produce the microecological preparation due to the lack of liquid fermentation and post-processing equipment and professional technicians, but the content of effective microorganisms of the microecological product produced by the method is low, the microecological product is easily polluted by mixed bacteria, and the quality is difficult to control. Therefore, simplifying the liquid fermentation process has important economic value.
At present, there are two main production modes of photosynthetic bacteria. One method is open-air culture, namely, a sealed transparent plastic bottle is used for fermenting strains, the fermentation amount is small, the strain is more applied to small and micro enterprises and farmers, and the strain is a main production method in photosynthetic bacteria culture production. The other method is fermentation tank production, which is mainly used in large-scale enterprises, and the production mode is relatively few because the cost is high, the operation is complex, and the purity requirement of photosynthetic bacteria and the like used for water adjustment on the culture is not high. However, in any production mode, the culture medium produced by the method needs to be carefully selected, so that the formula of the culture medium is one of the core components for determining the production of the photosynthetic bacteria.
At present, there are many culture mediums for photosynthetic bacteria production culture, such as membrane concentrated biogas slurry of chinese patent CN202011561045.8, photosynthetic bacteria culture medium of CN201910902322.8, and photosynthetic bacteria culture microbial inoculum of CN 201810463733.7. However, the existing culture medium has complex formula and process, high requirements on storage and transportation conditions and high requirements on the grade of raw materials, and high cost is caused. The photosynthetic bacteria products are widely applied, but the production process is simple, and the requirements on purity and the like are not high, so the cost of the photosynthetic bacteria products depends on a culture medium and production operation to a great extent.
Disclosure of Invention
In order to overcome the defects of the prior art, the invention provides a simple photosynthetic bacteria culture medium, which simplifies the components of the traditional photosynthetic bacteria culture under the premise of ensuring vitamin nutrition and mineral nutrition, thereby greatly reducing the production cost, rapidly improving the production efficiency within a short time (3 days), rapidly obtaining higher yield and having good theoretical value and practical production significance.
In order to achieve the purpose, the invention adopts the technical scheme that:
the invention provides a simple photosynthetic bacteria culture medium, which comprises sodium acetate, ammonium chloride, magnesium sulfate, potassium dihydrogen phosphate, ferrous chloride, calcium chloride and vitamins, wherein the vitamins comprise VB1Nicotinic acid VB3P-aminobenzoic acid, biotin and VB12(ii) a The purity of each raw material is agricultural fertilizer grade, and the pH value of the culture medium is 6.5-8.2.
The culture medium of photosynthetic bacteria generally includes essential carbon sources, nitrogen sources, minerals, and microbial nutrients. The carbon source of the photosynthetic bacteria culture medium is bicarbonate at first, and the sodium bicarbonate is mainly sodium bicarbonate because the sodium bicarbonate is best soluble in the bicarbonate. However, the pH of the sodium bicarbonate solution is high, so that the growth of photosynthetic bacteria is influenced to a certain extent, and meanwhile, the utilization speed of bicarbonate is high, so that the later culture speed cannot be kept up with that of the bicarbonate, the final concentration of the cultured bacteria liquid is low, particularly the amount of active bacteria is low, and dead bacteria can be deposited at the bottom. Therefore, the carbon source of the photosynthetic bacteria culture medium is successively replaced by polysaccharides with complex components such as molasses and the like, and although the organic carbon source with complex components can well balance various nutritional requirements of the photosynthetic bacteria in the growth process, the polysaccharide components are complex and are influenced by the sugar-making process, so that the supply of the polysaccharides cannot keep pace. Later, classical Ormemerod media used sodium malate as a carbon source, but was replaced very early by sodium lactate and sodium acetate due to the higher cost. Sodium lactate and sodium acetate have almost the same effect on the growth of photosynthetic bacteria, but the cost of the former is about 4 times that of the latter, so the former is commonly used in food processing, while the latter is used in the cultivation of photosynthetic bacteria.
In addition, a certain amount of trace elements are often required to be added into the photosynthetic bacteria culture medium, but the added trace elements are always the practice of the traditional yeast extract, although the effect is good, the cost is high, and the storage and the transportation are inconvenient, so that the raw material is gradually not suitable for the requirement of modern production.
The invention simplifies the formulation of the culture medium of the photosynthetic bacteria, uses the ion ammonium as the nitrogen source, and utilizes the CO generated by the carbon dioxide through respiration in the fermentation process3 2-、HCO3 -Equilibriously balance eight major ions (K)+、Na+、Ca2+、Mg2+、Cl-、SO4 2-、CO3 2-、HCO3 -) In the distribution of (1), Fe is also added2+，Ca2+And the addition of the vitamins is optimized, and finally a set of new production formula of the photosynthetic bacteria culture medium is developed, so that the production risk can be reduced, and the production efficiency can be improved. Meanwhile, various agricultural fertilizer grade raw materials (namely, industrial grade raw materials, and analytical purity is not needed) are used, and the original cost is reduced by about 70% (shown in table 1), so that the profit margin of the products is greatly widened. Because the components are simplified, the production operation intensity is reduced, and the production progress is greatly promoted. Has important significance in improving the batch culture of photosynthetic bacteria and producing practical application.
TABLE 1 cost accounting table for the price of each ingredient in the culture medium
Figure BDA0003299849220000031
Preferably, the simple photosynthetic bacteria culture medium is specifically: each liter of water contains 1.5 to 3g of sodium acetate, 1 to 1.5g of ammonium chloride, 0.6 to 1.4g of magnesium sulfate, 0.5 to 0.8g of monopotassium phosphate, 0.002 to 0.008g of ferrous chloride and 0.008 to 0.02g of calcium chloride; the addition amount of the vitamins is as follows: VB10.2-0.5g, nicotinic acid VB30.15-0.30g of p-aminobenzoic acid0.05-0.15g, biotin 0.04-0.10g and VB120.05-0.10 g; the purity of each raw material is agricultural fertilizer grade, and the pH value of the culture medium is 6.5-8.2.
As a preferred embodiment of the present invention, the simple photosynthetic bacteria culture medium is specifically: each liter of water contains 2g of sodium acetate, 1.2g of ammonium chloride, 0.8g of magnesium sulfate, 0.5g of monopotassium phosphate, 0.002g of ferrous chloride and 0.01g of calcium chloride; the addition amount of the vitamins is as follows: VB10.30g, nicotinic acid VB30.250g, 0.100g of p-aminobenzoic acid, 0.060g of biotin and VB120.070 g; the purity of each raw material is agricultural fertilizer grade.
Preferably, the pH of the medium is 7.2-7.8. Further, the pH of the medium was 7.8.
Preferably, the use method of the simple photosynthetic bacteria culture medium comprises the following steps: each vitamin is prepared in proportion and is packaged independently after being prepared into equal specifications; the ferrous chloride is packaged separately; the sodium acetate, the ammonium chloride, the magnesium sulfate, the monopotassium phosphate and the calcium chloride are prepared in proportion and then are independently packaged after being prepared into equal specifications; when in use, the components are mixed together in proportion to prepare the culture medium.
The invention also provides application of the simple photosynthetic bacteria culture medium in enrichment and separation of photosynthetic bacteria.
Preferably, when the photosynthetic bacteria are isolated, the culture medium is prepared into a double-layer culture medium, the double-layer culture medium comprises an upper-layer culture medium and a lower-layer culture medium, the upper-layer culture medium is a full-solid culture medium prepared by adding agar, and the lower-layer culture medium is a semi-solid culture medium prepared by adding agar.
Compared with the prior art, the invention has the beneficial effects that:
the invention provides a simple photosynthetic bacteria culture medium, which comprises sodium acetate, ammonium chloride, magnesium sulfate, potassium dihydrogen phosphate, ferrous chloride, calcium chloride and vitamins, wherein the vitamins comprise p-aminobenzoic acid, biotin and VB3、VB12And VB1The culture medium has simple and practical formula and high production efficiency, and can be used for the medicinesThe grade of the strain has no special requirements, the strain can reach the agricultural fertilizer grade, the raw materials are easy to obtain, cheap and stable in source, the strain has the same enrichment effect on rhodospirillaceae photosynthetic bacteria such as rhodopseudomonas palustris, rhodobacter capsulatus, rhodospirillum rubrum and the like in a suitable condition range, and the viable count can reach 1 multiplied by 10 in 3 days9The method is suitable for large-scale production, is applied to culture enrichment and separation of photosynthetic bacteria, can accelerate culture, improves production efficiency, and has good theoretical value and practical production significance.
Detailed Description
The following further describes the embodiments of the present invention. It should be noted that the description of the embodiments is provided to help understanding of the present invention, but the present invention is not limited thereto. In addition, the technical features involved in the embodiments of the present invention described below may be combined with each other as long as they do not conflict with each other.
The experimental procedures in the following examples were carried out by conventional methods unless otherwise specified, and the test materials used in the following examples were commercially available by conventional methods unless otherwise specified.
Example 1A simple photosynthetic bacteria culture Medium
Each liter of water contains 2g of sodium acetate, 1.2g of ammonium chloride, 0.8g of magnesium sulfate, 0.5g of monopotassium phosphate, 0.002g of ferrous chloride and 0.01g of calcium chloride.
The addition amount of the vitamins is as follows: VB10.30g, nicotinic acid VB30.250g, 0.100g of p-aminobenzoic acid, 0.060g of biotin and VB120.070 g; the pH value is 7.2-7.8; the purity of the raw materials is agricultural fertilizer grade.
Various vitamins are prepared in proportion and packaged independently after being prepared into equal specifications; the ferrous chloride is required to be packaged separately; sodium acetate, ammonium chloride, magnesium sulfate, potassium dihydrogen phosphate and calcium chloride are prepared in proportion and packaged separately. When in use, the components are mixed together in proportion to prepare the culture medium.
The present medium can be used directly in the enrichment. The culture medium needs to be prepared into a double-layer culture medium in the culture and separation processes: the upper layer culture medium is a full solid culture medium, namely 20g of agar is added in each liter of water on the basis of the culture medium; the lower layer is semisolid culture medium, namely 10g of agar is added in each liter of water on the basis of the culture medium.
Example 2A simple photosynthetic bacteria culture Medium
Each liter of water contains 1.5g of sodium acetate, 1g of ammonium chloride, 0.6g of magnesium sulfate, 0.5g of monopotassium phosphate, 0.002g of ferrous chloride and 0.008g of calcium chloride;
the addition amount of the vitamins is as follows: VB10.2g, nicotinic acid VB30.15g, 0.05g of p-aminobenzoic acid, 0.04g of biotin and VB120.05 g; the pH value is 7.2-7.8; the purity of the raw materials is agricultural fertilizer grade.
Various vitamins are prepared in proportion and packaged independently after being prepared into equal specifications; the ferrous chloride is required to be packaged separately; sodium acetate, ammonium chloride, magnesium sulfate, potassium dihydrogen phosphate and calcium chloride are prepared in proportion and packaged separately. When in use, the components are mixed together in proportion to prepare the culture medium.
The present medium can be used directly in the enrichment. The culture medium needs to be prepared into a double-layer culture medium in the culture and separation processes: the upper layer culture medium is a full solid culture medium, namely 20g of agar is added in each liter of water on the basis of the culture medium; the lower layer is semisolid culture medium, namely 10g of agar is added in each liter of water on the basis of the culture medium.
Example 3A simple photosynthetic bacteria culture Medium
Each liter of water contains 3g of sodium acetate, 1.5g of ammonium chloride, 1.4g of magnesium sulfate, 0.8g of monopotassium phosphate, 0.008g of ferrous chloride and 0.02g of calcium chloride;
the addition amount of the vitamins is as follows: VB10.5g, nicotinic acid VB30.30g, 0.15g of p-aminobenzoic acid, 0.10g of biotin and VB120.10 g; the pH value is 7.2-7.8; the purity of the raw materials is agricultural fertilizer grade.
Various vitamins are prepared in proportion and packaged independently after being prepared into equal specifications; the ferrous chloride is required to be packaged separately; sodium acetate, ammonium chloride, magnesium sulfate, potassium dihydrogen phosphate and calcium chloride are prepared in proportion and packaged separately. When in use, the components are mixed together in proportion to prepare the culture medium.
The present medium can be used directly in the enrichment. The culture medium needs to be prepared into a double-layer culture medium in the culture and separation processes: the upper layer culture medium is a full solid culture medium, namely 20g of agar is added in each liter of water on the basis of the culture medium; the lower layer is semisolid culture medium, namely 10g of agar is added in each liter of water on the basis of the culture medium.
Comparative example 1 mineral-free photosynthetic bacteria culture Medium
Each liter of water contains 2g of sodium acetate, 1.2g of ammonium chloride, 0.8g of magnesium sulfate and 0.5g of monopotassium phosphate.
The addition amount of the vitamins is as follows: VB10.30g, nicotinic acid VB30.250g, 0.100g of p-aminobenzoic acid, 0.060g of biotin and VB12 0.070g。
Experimental example 1 culturing Effect of the photosynthetic bacterium culture Medium of the present invention on Rhodopseudomonas palustris
Rhodopseudomonas palustris (Rhodopseudomonas palustris) was cultured from 2021, 5/28 using the medium of example 1, the medium of comparative example 1, and a commercial photosynthetic bacteria medium (purchased from Lvjinggang, Nanjing).
During the culture process, the culture medium is prepared in a 1000L fermentation tank according to the formula of each culture medium, the pH of the culture medium is 7.8, and then 30 percent (volume percentage) of the pre-cultured rhodopseudomonas palustris bacterial liquid (the concentration is 1.0 multiplied by 10)9CFU/mL), stirring uniformly, directly pouring into a 10L transparent plastic bottle, sampling on site, detecting an initial light absorption value by using an ultraviolet spectrophotometer at a wavelength of 570nm, then placing under natural light for culturing, appropriately shading, keeping the illumination at about 6000Lx at the temperature of 28 ℃, culturing for 3h, 23h, 46.5h and 67h, and then respectively sampling and detecting (three times of measurement of each experimental group are repeated).
As shown in Table 2, the final concentrations of the bacterial liquids of the culture mediums of the present invention were all greater than 2.0X 1010CFU/mL(OD>1.6). The growth state can be maintained at 67 hours (about 3 days), which is superior to mineral-free photosynthetic bacteria culture medium and commercialized photosynthetic bacteriaAnd (4) a culture medium.
TABLE 2 statistics of experimental results of different culture media for large-scale culture of Rhodopseudomonas palustris
Figure BDA0003299849220000061
Experimental example 2 culturing Effect of the photosynthetic bacterium culture medium of the present invention on rhodobacter capsulatus, rhodopseudomonas palustris and rhodospirillum rubrum
From 2021, 5/31, Rhodobacter capsulatus (Rhodobacter capsulatus), Rhodopseudomonas palustris (Rhodopseudomonas palustris), Rhodospirillum rubrum (Rhodospirillum rubrum) were cultured using the medium of example 1, and labeled as RC group, RP group, and RR group, respectively.
In the culture process, the culture medium is prepared in a 1000L fermentation tank according to the formula of the culture medium, the prepared liquid is mixed uniformly for culture, the pH value of the culture medium is 7.8, then 30% of bacterial liquid (1.0 multiplied by 109CFU/mL) is added into each experimental group, the mixture is stirred uniformly and then is directly poured into a 10L transparent plastic bottle, sampling is carried out on site, an ultraviolet spectrophotometer is used for detecting the light absorption value under the wavelength of 570nm, then the mixture is placed under natural light for culture, proper shading is carried out, the illumination is maintained at about 6000Lx, the temperature is maintained at 28-30 ℃, and the sampling detection is carried out after 3.5h, 14h, 22h, 44h, 60h, 86h and 112h (4.5d) of culture are carried out respectively.
As shown in Table 3, the culture time of all three photosynthetic bacteria after 4.5 days of culture was 1.0X 1010CFU/ml(OD>1.5) above.
TABLE 3 statistics of the detection data of the large-scale cultivation experiments of different photosynthetic bacteria
Figure BDA0003299849220000071
Experimental example 3 Effect of the culture Medium of the present invention on isolation of photosynthetic bacteria
The sludge of the flower land river is subjected to photosynthetic bacteria separation in 2021 year 3-4 months. Adding 15g of river bottom sludge into 50mL of water, shaking, precipitating, taking supernatant, adding the supernatant into a 250mL sterile conical flask, filling the flask with the culture medium of the embodiment 1, sealing the flask opening, and placing the flask in an incubator for anaerobic enrichment culture at 30 ℃ under illumination of 3500-4500 Lx. After the liquid culture is red or reddish brown, the liquid culture is transferred to a new culture medium (the culture medium of example 1) and repeated for 2-3 times. And (3) carrying out gradient dilution on the enriched bacterial liquid, mixing the bacterial liquid with a proper dilution with the lower layer of the double-layer culture medium cooled to 40-50 ℃, then injecting the mixture into a dish to prepare a thin (1-2cm) layer of flat plate, injecting the upper layer of culture medium after the lower layer of culture medium is solidified, and filling the whole dish. After the double-layer flat plate method is adopted for multiple separation, pure bacterial colonies with consistent bacterial colony forms are obtained, the pure bacterial colonies are oval, smooth and wet in surface, 2-3 mm in diameter, neat in edge and light yellow to bright red. The bacterial liquid is bright red and aerobic under the conditions of sufficient illumination and anaerobic condition, and the bacterial liquid is turbid and has a little precipitate. Finally, the strain was inoculated by puncturing in a semi-solid medium (prepared by adding agar to the medium of example 1) and stored for future use.
The bacterium is gram-stained and is negative, cells are egg-shaped, and when the bacterium is observed under a 16 x 100-fold optical microscope, coccoid bacteria with the size of 2.5-3.5 microns x 2 microns can be seen, and polar flagella can rapidly move and propagate in a dichotomous mode.
The external morphology and 16sRNA sequence were examined, and the results showed that the bacteria may be Rhodobacter sphaeroides (Rhodobacter sphaeroides), a photosynthetic bacteria used in environmental protection, commonly used for the removal of harmful heavy metals (azalea, Zhang, Popule's moth, etc.. Rhodobacter sphaeroides for the conversion removal of heavy metal cadmium and its mechanism research [ J ]. environmental science, 2006,26(11): 1809. alkanone 1814.) and the degradation of organic pollutants (Wangyifen, Zhang, picrinine, etc.. Rhodobacter sphaeroides for the anaerobic degradation of o-dichlorobenzene and its mechanism research [ J.. microbiology, 2008(05):23-30), etc.
The embodiments of the present invention have been described in detail, but the present invention is not limited to the described embodiments. It will be apparent to those skilled in the art that various changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, and the scope of protection is still within the scope of the invention.

Claims (7)

1. A simple photosynthetic bacteria culture medium is characterized by comprising sodium acetate, ammonium chloride, magnesium sulfate, potassium dihydrogen phosphate, ferrous chloride, calcium chloride and vitamins, wherein the vitamins comprise VB1Nicotinic acid VB3P-aminobenzoic acid, biotin and VB12(ii) a The purity of each raw material is agricultural fertilizer grade, and the pH value of the culture medium is 6.5-8.2.
2. A simple photosynthetic bacteria culture medium according to claim 1 wherein each liter of water contains 1.5-3g of sodium acetate, 1-1.5g of ammonium chloride, 0.6-1.4g of magnesium sulfate, 0.5-0.8g of monopotassium phosphate, 0.002-0.008g of ferrous chloride, 0.008-0.02g of calcium chloride; the addition amount of the vitamins is as follows: VB10.2-0.5g, nicotinic acid VB30.15-0.30g, 0.05-0.15g of p-aminobenzoic acid, 0.04-0.10g of biotin and VB120.05-0.10 g; the purity of each raw material is agricultural fertilizer grade, and the pH value of the culture medium is 6.5-8.2.
3. A simple photosynthetic bacteria culture medium according to claim 2 comprising, per liter of water, 2g of sodium acetate, 1.2g of ammonium chloride, 0.8g of magnesium sulfate, 0.5g of monopotassium phosphate, 0.002g of ferrous chloride, 0.01g of calcium chloride; the addition amount of the vitamins is as follows: VB10.30g, nicotinic acid VB30.250g, 0.100g of p-aminobenzoic acid, 0.060g of biotin and VB120.070 g; the purity of each raw material is agricultural fertilizer grade.
4. A compact photosynthetic bacteria culture medium according to claim 2 wherein the pH of the culture medium is 7.2-7.8.
5. A simplified photosynthetic bacteria culture medium according to any one of claims 1 to 4 wherein the culture medium is used by the method comprising: each vitamin is prepared in proportion and is packaged independently after being prepared into equal specifications; the ferrous chloride is packaged separately; the sodium acetate, the ammonium chloride, the magnesium sulfate, the monopotassium phosphate and the calcium chloride are prepared in proportion and then are independently packaged after being prepared into equal specifications; when in use, the components are mixed together in proportion to prepare the culture medium.
6. Use of a reduced photosynthetic bacteria culture medium of any one of claims 1 to 5 for culture enrichment and isolation of photosynthetic bacteria.
7. The use of claim 6, wherein the culture medium is prepared into a double-layer culture medium when the photosynthetic bacteria are isolated, the double-layer culture medium comprises an upper layer culture medium and a lower layer culture medium, the upper layer culture medium is a full solid culture medium prepared by adding agar, and the lower layer culture medium is a semi-solid culture medium prepared by adding agar.
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