CN106479932A - A kind of Lactobacillus pentosus producing gamma aminobutyric acid - Google Patents
A kind of Lactobacillus pentosus producing gamma aminobutyric acid Download PDFInfo
- Publication number
- CN106479932A CN106479932A CN201611003216.9A CN201611003216A CN106479932A CN 106479932 A CN106479932 A CN 106479932A CN 201611003216 A CN201611003216 A CN 201611003216A CN 106479932 A CN106479932 A CN 106479932A
- Authority
- CN
- China
- Prior art keywords
- lactobacillus pentosus
- aminobutyric acid
- gamma aminobutyric
- lactobacillus
- pentosus
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
- C12N1/205—Bacterial isolates
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
- C12R2001/225—Lactobacillus
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P13/00—Preparation of nitrogen-containing organic compounds
- C12P13/005—Amino acids other than alpha- or beta amino acids, e.g. gamma amino acids
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Medicinal Chemistry (AREA)
- Tropical Medicine & Parasitology (AREA)
- Virology (AREA)
- Biomedical Technology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention provides a kind of Lactobacillus pentosus SS6 producing gamma aminobutyric acid, described Lactobacillus pentosus SS6 is deposited in Guangdong Province's Culture Collection, and preservation address is Guangdong Microbes Inst（5th floor, Xianlie Middle Road, Guangzhou City Road 100 No. 59 building of compound）, preservation date is on October 13rd, 2016, and deposit number is GDMCC No：60086；The yield that gamma aminobutyric acid is produced in Lactobacillus pentosus SS6 fermentation reaches 3.09mg/mL, has the ability of higher product gamma aminobutyric acid, and the food rich in gamma aminobutyric acid for developing new provides the foundation.
Description
Technical field
The present invention relates to microbial technology field, more particularly, to a kind of Lactobacillus pentosus producing γ-aminobutyric acid.
Background technology
γ-aminobutyric acid（GABA）There are many important physiological functions, such as anxiety, blood pressure lowering, blood sugar lowering, improvement is slept
Sleep, repair skin（Suppression inflammation, stimulates reepithelialization）, adjust hormone etc..At this stage with to GABA physiology and pharmacology
The continuous research and development of function, is applied in the industries such as food and medicine as a kind of new functional component.
In early stage, enrichment produces γ-aminobutyric acid mainly with chemosynthesis, plants enriched and by Escherichia coli fermentation be
Main, but there is poor stability, high cost, environmental pollution is serious, and enrichment content is low and the problems such as edible safety, so searching
Available edible level security bacterial strain has potential meaning and value in terms of microbial method enriching gamma-aminobutyric.
Content of the invention
The technical problem to be solved is the drawbacks described above overcoming prior art to exist, and provides a kind of product γ-ammonia
The Lactobacillus pentosus of base butanoic acid.
The purpose of the present invention is achieved by the following technical programs：
A kind of Lactobacillus pentosus SS6 producing γ-aminobutyric acid, described Lactobacillus pentosus SS6 are deposited in Guangdong Province microorganism fungus kind
Collection, preservation address is Guangdong Microbes Inst（5th floor, Xianlie Middle Road, Guangzhou City Road 100 No. 59 building of compound）,
Preservation date is on October 13rd, 2016, and deposit number is GDMCC No：60086.
Lactobacillus pentosus of the present invention（Lactobacillus pentosus）The sequence of the 16s rDNA of SS6 such as SEQ ID
NO：Shown in 1, by 16S rDNA sequence alignment analysis, with the Lactobacillus pentosus PIS23 bacterial strain of Serial No. JN415155.1
The homology highest of 16S rDNA sequence, similarity is 99%, by with reference to morphological features, growth conditionss, Physiology and biochemistry mirror
Determine result and determine that Lactobacillus pentosus SS6 belongs to Lactobacillus（Lactobacillus）, it is Lactobacillus pentosus subspecies
（Lactobacillus pentosus）.
Described Lactobacillus pentosus SS6 is gram positive bacteria, shaft-like, atrichia, sterile film, no pod membrane；Long 1.5～3.0 μ
M, wide 0.7～0.8 μm, bacterium colony is rounded, and color is milky, and smooth surface is opaque, neat in edge.
Lactobacillus pentosus SS6 is anaerobe, and catalase test is negative, and oxidase test is negative, gelatin liquefaction test
It is negative, hydrogen sulfide production test is negative, indole test is negative, casein hydrolysis experiment is negative, it is in the moon that arginine produces ammonia test
Property, urease test is negative, and Esculin hydrolysis experiment is positive.
Lactobacillus pentosus SS6 can grow in MRS culture medium, and its optimum growth temperature is 35 DEG C, the most suitable growth environment
PH value is 5.8～6.5.
Lactobacillus pentosus SS6 of the present invention powder of Folium Mori conversion of fermenting produces γ-aminobutyric acid, and result of the test shows to add
Plus the γ-aminobutyric acid yield that Lactobacillus pentosus SS6 is fermented to powder of Folium Mori is the 387.5% of blank powder of Folium Mori matched group, it is
The food rich in γ-aminobutyric acid for the exploitation provides foundation.
The present invention also provides the microorganism formulation containing described Lactobacillus pentosus SS6.
Compared with prior art, the invention has the advantages that：
The invention provides a kind of Lactobacillus pentosus SS6 producing γ-aminobutyric acid, described Lactobacillus pentosus SS6 is deposited in Guangdong
Save Culture Collection, preservation address is Guangdong Microbes Inst（Xianlie Middle Road, Guangzhou City Road 100 compound
5th floor, No. 59 building）, preservation date is on October 13rd, 2016, and deposit number is GDMCC No：60086；Lactobacillus pentosus SS6 sends out
The yield that ferment produces γ-aminobutyric acid reaches 3.09mg/mL, has the ability of higher product γ-aminobutyric acid, for developing new richness
Food containing gamma aminobutyric acid provides the foundation.
Brief description
Fig. 1 is Lactobacillus pentosus（Lactobacillus pentosus）The 16s rDNA sequence of SS6 and close bacterial strain is entered
The phylogenetic tree that row sequence analysis build.
Specific embodiment
Further illustrate present disclosure with reference to Figure of description and specific embodiment, but should not be construed as to this
The restriction of invention.Without departing from the spirit and substance of the case in the present invention, that the inventive method, step or condition are made is simple
Modification or replacement, belong to the scope of the present invention；If not specializing, in embodiment, technological means used are art technology
Conventional meanses known to personnel.
Lactic acid bacteria isolation medium（1000mL）By 10g yeast extract, 10g Carnis Bovis seu Bubali cream, 10g peptone, 5g glucose, 1ml
Tween 80,20g Calcium Carbonate, 200g Tomato juice, the distilled water composition of 0.1g bromocresol green, 15g agar powder and surplus, are adjusted
PH to 5.8～6.5, in 121 DEG C of autoclaving 30min.
MRS culture medium（1000mL）By 10g peptone, 10g Carnis Bovis seu Bubali cream, 5g yeast extract, 2g diammonium hydrogen citrate [(NH4)2HC6H5O7], 20g glucose, 1mL tween 80,5g sodium acetate（CH3COONa-3H2O）, 2g dipotassium hydrogen phosphate（K2HPO4·
3H2O）, 0.58g magnesium sulfate（MgSO4.7H2O）, 0.25g magnesium sulfate（MnSO4·H2O）, the distilled water group of 15g agar powder and surplus
Become, adjust pH to 5.8～6.5, in 121 DEG C of autoclaving 30min.
MRS fluid medium（1000mL）By 10g peptone, 10g Carnis Bovis seu Bubali cream, 5g yeast extract, 2g diammonium hydrogen citrate
[(NH4)2HC6H5O7], 20g glucose, 1mL tween 80,5g sodium acetate（CH3COONa-3H2O）, 2g dipotassium hydrogen phosphate
（K2HPO4·3H2O）, 0.58g magnesium sulfate（MgSO4.7H2O）, 0.25g magnesium sulfate（MnSO4·H2O）Distilled water group with surplus
Become, adjust pH to 5.8～6.5, in 121 DEG C of autoclaving 30min.
Fermentation medium TYG（1000mL）By 5g yeast extract, 5g tryptone, 10g glucose, 5g sodium succinate,
The distilled water composition of 20mg L-Glu and surplus, adjusts pH to 5.8～6.5, in 121 DEG C of autoclaving 30min.
The separation of embodiment 1 bacterial strain, screening and identification
Strains separation：Choose the ripe mulberry fruit on healthy growth mulberry, with after aseptic water washing 3 times in aseptic super-clean bench
Use aseptic filter paper suck dry moisture, be cut into 1cm small fragment and be placed in grinding juice, draw 100 μ L fruit juice in MRS fluid medium
In, cultivate 2d in 30 DEG C of constant incubators, treat that fluid medium becomes muddy and dilutes bacterium solution by gradient dilution method, draw dilution factor
For 10-5With 10-6Mycelium dilution liquid 100 μ L in lactic acid bacteria isolation medium, 30 DEG C of constant incubators cultivate 2～3d, treat breast
Bacterium colony is grown in sour bacterium isolation medium（Circular milky white or yellow color colonies occur）, surrounding media is changed into yellow person and primarily determines that
For bacterial strain SS6, the maximum bacterium colony of picking change chromosphere is in MRS culture medium culturing.
The bacterial strain SS6 that screening is obtained carries out Molecular Identification, student on commission work biological engineering (Shanghai) limited company pair
The 16S rDNA screening the SS6 obtaining is sequenced.
The 16S rDNA sequence length of bacterial strain SS6 is 1472bp, its sequence such as SEQ ID NO：Shown in 1, by sequencing result
Carry out sequence analysis with the 16S rDNA sequence in GenBank, then use software building phylogenetic tree（As shown in Figure 1）,
To determine the race relation of bacterial strain.Homology analysis result shows, this sequence newborn bar with the pentose of Serial No. JN415155.1
The homology highest of the 16S rDNA sequence of bacterium PIS23 bacterial strain, similarity is 99%, by entrusting Guangdong Province's microbiological analysiss inspection
With reference to thalli morphology, growth conditionss, Physiology and biochemistry qualification result, measured center determines that bacterial strain SS6 belongs to Lactobacillus
（Lactobacillus）, Lactobacillus pentosus（Lactobacillus pentosus）Subspecies, therefore referred to as Lactobacillus pentosus SS6.
Bacterial strain colonial morphology is identified：Lactobacillus pentosus SS6 is gram positive bacteria, shaft-like, atrichia, no pod membrane；In MRS
Cultivate on solid medium, bacterium colony is rounded, color is milky, smooth surface is opaque, neat in edge.
Reference《The outstanding Bacteria Identification handbook of uncle》9th edition and《Common bacteria system identification handbook》（East show pearl chief editor's science
Publishing house）Physiology and biochemistry identification is carried out to Lactobacillus pentosus SS6：Lactobacillus pentosus SS6 is anaerobe, and catalase test is in the moon
Property, oxidase test is negative, and gelatin liquefaction test is negative, and hydrogen sulfide production test is negative, and indole test is negative, casein water
Solution test is negative, and arginine produces ammonia test and is negative, and urease test is negative, and Esculin hydrolysis experiment is positive, and result is shown in
Table 1.
With young age slant culture percutaneous puncture-inoculation in stopping and the sharp gloomy Er Shi of husband（Semi-solid）In culture medium, 37 DEG C of constant temperature culture
Observe after 72h.As indicator turns yellow, represent and produce acid, for the positive；Constant or change is blue（Purple）It is then feminine gender, result such as table 2.
Lactobacillus pentosus SS6 can grow in MRS culture medium, and its optimum growth temperature is 35 DEG C, the most suitable growth environment
PH value is 5.8～6.5.
Lactobacillus pentosus（Lactobacillus pentosus）SS6, is deposited in Guangdong Province's Culture Collection
（GDMCC）, preservation address is Guangdong Microbes Inst, 5th floor, Xianlie Middle Road, Guangzhou City Road 100 No. 59 building of compound, protects
The Tibetan date is on October 13rd, 2016, and deposit number is GDMCC No：60086.
The culture of embodiment 2 bacterial strain
1st, the activation of Lactobacillus pentosus SS6：Sterile working in superclean bench, with inoculating loop picking Lactobacillus pentosus SS6,
Plating medium（MRS culture medium）On use inoculating loop line, quiescent culture 2d in 30 DEG C of incubators.
2nd, the preparation of Lactobacillus pentosus SS6 fermented sample：It is inoculated in 50mL fermentation by separating the Lactobacillus pentosus SS6 obtaining
In culture medium TYG, quiescent culture 2d in 30 DEG C of incubators, by fermenting mixture boiling water bath 10min, with 10000r/min centrifugation
10min, takes supernatant liquid to obtain final product fermentation liquid.
3rd, the drafting of γ-aminobutyric acid standard curve：Take respectively 1.0mL distilled water, concentration be 1mg/mL, 2mg/mL,
The standard sample solution of the γ-aminobutyric acid of 3mg/mL, 4mg/mL, 5mg/mL, adds 120 μ L 1mol/L LaCL3Solution, mixes
Even concussion reaction 15min, 12 000 r/min are centrifuged 5 min, take supernatant 0.5 mL in 1.5mL centrifuge tube, add 300 L
The KOH of 1mol/L, oscillating reactionss 5 min, 12 000 r/min is centrifuged 5 min.Supernatant 600 L is taken to be placed in 15mL tool respectively
In plug test tube, plus carbonate buffer solution 400 L of pH 10.0,60% re-distilled phenol 200 L, add 1mL 7.5% after mixing
NaClO solution, fully mixes.Stand 3 min, heat 10 min in boiling water bath, 10 min in rearmounted ice bath, treat that solution occurs
After aeruginouss, add 4.0 mL 60% ethanol solution, with No. 0 pipe for comparison, survey light absorption value at 645 nm wavelength.With light absorption value
For vertical coordinate, GABA concentration（mg/mL）For abscissa, draw standard curve.
4th, take the fermentation liquid of 1mL Lactobacillus pentosus to be measured SS6, add by above-mentioned steps and measure at wavelength 645nm after reagent
Light absorption value, make 3 repetition, according to standard curve calculate sample γ-aminobutyric acid content.
Result of the test shows：The yield that γ-aminobutyric acid is produced in Lactobacillus pentosus SS6 fermentation reaches 3.09mg/mL, has higher
Product γ-aminobutyric acid ability, provide foundation for developing the new food rich in gamma aminobutyric acid.
SEQUENCE LISTING
<110>Agricultural University Of South China
<120>A kind of Lactobacillus pentosus producing γ-aminobutyric acid
<130>
<160> 1
<170> PatentIn version 3.3
<210> 1
<211> 1472
<212> DNA
<213>The 16S rDNA sequence of Lactobacillus pentosus SS6
<400> 1
cccgtggcgg gctgccctat acatgcaagt cgaacgaact ctggtattga ttggtgcttg 60
catcatgatt tacatttgag tgagtggcga actggtgagt aacacgtggg aaacctgccc 120
agaagcgggg gataacacct ggaaacagat gctaataccg cataacaact tggaccgcat 180
ggtccgagtt tgaaagatgg cttcggctat cacttttgga tggtcccgcg gcgtattagc 240
tagatggtgg ggtaacggct caccatggca atgatacgta gccgacctga gagggtaatc 300
ggccacattg ggactgagac acggcccaaa ctcctacggg aggcagcagt agggaatctt 360
ccacaatgga cgaaagtctg atggagcaac gccgcgtgag tgaagaaggg tttcggctcg 420
taaaactctg ttgttaaaga agaacatatc tgagagtaac tgttcaggta ttgacggtat 480
ttaaccagaa agccacggct aactacgtgc cagcagccgc ggtaatacgt aggtggcaag 540
cgttgtccgg atttattggg cgtaaagcga gcgcaggcgg ttttttaagt ctgatgtgaa 600
agccttcggc tcaaccgaag aagtgcatcg gaaactggga aacttgagtg cagaagagga 660
cagtggaact ccatgtgtag cggtgaaatg cgtagatata tggaagaaca ccagtggcga 720
aggcggctgt ctggtctgta actgacgctg aggctcgaaa gtatgggtag caaacaggat 780
tagataccct ggtagtccat accgtaaacg atgaatgcta agtgttggag ggtttccgcc 840
cttcagtgct gcagctaacg cattaagcat tccgcctggg gagtacggcc gcaaggctga 900
aactcaaagg aattgacggg ggcccgcaca agcggtggag catgtggttt aattcgaagc 960
tacgcgaaga accttaccag gtcttgacat actatgcaaa tctaagagat tagacgttcc 1020
cttcggggac atggatacag gtggtgcatg gttgtcgtca gctcgtgtcg tgagatgttg 1080
ggttaagtcc cgcaacgagc gcaaccctta ttatcagttg ccagcattaa gttgggcact 1140
ctggtgagac tgccggtgac aaaccggagg aaggtgggga tgacgtcaaa tcatcatgcc 1200
ccttatgacc tgggctacac acgtgctaca atggatggta caacgagttg cgaactcgcg 1260
agagtaagct aatctcttaa agccattctc agttcggatt gtaggctgca actcgcctac 1320
atgaagtcgg aatcgctagt aatcgcggat cagcatgccg cggtgaatac gttcccgggc 1380
cttgtacaca ccgcccgtca caccatgaga gtttgtaaca cccaaagtcg gtggggtaac 1440
cttttaggaa ccagccgcta agtgacagat gg 1472
Claims (4)
1. a kind of Lactobacillus pentosus SS6 producing γ-aminobutyric acid is it is characterised in that described Lactobacillus pentosus SS6 is deposited in Guangdong
Save Culture Collection, preservation address is Guangdong Microbes Inst, preservation date is on October 13rd, 2016, protects
Hiding numbering is GDMCC No：60086.
2. according to claim 1 produce γ-aminobutyric acid Lactobacillus pentosus SS6 it is characterised in that described pentose breast bar
Bacterium SS6 is gram positive bacteria, shaft-like, atrichia, no pod membrane；Bacterium colony is rounded, and color is milky, and smooth surface is impermeable
Bright, neat in edge.
3. the Lactobacillus pentosus SS6 producing γ-aminobutyric acid according to claim 1 is it is characterised in that described pentose is newborn
The sequence of the 16s rDNA of bacillus SS6 such as SEQ ID NO：Shown in 1.
4. the microorganism formulation containing Lactobacillus pentosus SS6 described in claim 1.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611003216.9A CN106479932B (en) | 2016-11-15 | 2016-11-15 | A kind of Lactobacillus pentosus producing γ-aminobutyric acid |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611003216.9A CN106479932B (en) | 2016-11-15 | 2016-11-15 | A kind of Lactobacillus pentosus producing γ-aminobutyric acid |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN106479932A true CN106479932A (en) | 2017-03-08 |
| CN106479932B CN106479932B (en) | 2019-08-16 |
Family
ID=58272060
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201611003216.9A Active CN106479932B (en) | 2016-11-15 | 2016-11-15 | A kind of Lactobacillus pentosus producing γ-aminobutyric acid |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106479932B (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107048364A (en) * | 2017-04-17 | 2017-08-18 | 华南农业大学 | A kind of mulberry leaf powder rich in gamma aminobutyric acid and preparation method thereof |
| CN113832085A (en) * | 2021-11-29 | 2021-12-24 | 北京量化健康科技有限公司 | Lactobacillus pentosus BNPV and application thereof |
| CN114774312A (en) * | 2021-12-01 | 2022-07-22 | 中国水产科学研究院珠江水产研究所 | Whale bacillus NK01 and application thereof |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105754887A (en) * | 2014-12-16 | 2016-07-13 | 勐海茶业有限责任公司 | Lactobacillus pentosus for producing gamma-aminobutyric acid and its use in Pu'er tea production |
-
2016
- 2016-11-15 CN CN201611003216.9A patent/CN106479932B/en active Active
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105754887A (en) * | 2014-12-16 | 2016-07-13 | 勐海茶业有限责任公司 | Lactobacillus pentosus for producing gamma-aminobutyric acid and its use in Pu'er tea production |
Non-Patent Citations (1)
| Title |
|---|
| 管立军等: "产γ-氨基丁酸乳酸菌的分离及分离鉴定", 《食品科技》 * |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107048364A (en) * | 2017-04-17 | 2017-08-18 | 华南农业大学 | A kind of mulberry leaf powder rich in gamma aminobutyric acid and preparation method thereof |
| CN107048364B (en) * | 2017-04-17 | 2020-12-29 | 华南农业大学 | Mulberry leaf powder rich in gamma-aminobutyric acid and preparation method thereof |
| CN113832085A (en) * | 2021-11-29 | 2021-12-24 | 北京量化健康科技有限公司 | Lactobacillus pentosus BNPV and application thereof |
| CN114774312A (en) * | 2021-12-01 | 2022-07-22 | 中国水产科学研究院珠江水产研究所 | Whale bacillus NK01 and application thereof |
| CN114774312B (en) * | 2021-12-01 | 2022-10-21 | 中国水产科学研究院珠江水产研究所 | Whale bacillus NK01 and application thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| CN106479932B (en) | 2019-08-16 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN112760271B (en) | Process for producing clostridium butyricum through high-density fermentation under negative pressure condition and application | |
| CN110283743B (en) | Degrading bacterium for degrading allelochemicals in plant root exudates and application thereof | |
| CN114015729B (en) | Application of lactobacillus plantarum in preparation of lactobacillin, lactic acid and phenyllactic acid | |
| CN116286468A (en) | Lactobacillus mucilaginosus LF-ONLLY with antioxidant function and application thereof in fermented food | |
| CN106479932B (en) | A kind of Lactobacillus pentosus producing γ-aminobutyric acid | |
| CN104480187A (en) | High-yield gamma-aminobutyric acid lactobacillus and screening method thereof | |
| CN105361161A (en) | Making method of cordyceps militaris kiwi enzyme capable of enhancing immunity | |
| CN103937691B (en) | One plant production β fructosidases aspergillus oryzae strain and its cultural method and application | |
| CN103289925B (en) | Lactic acid bacteria strain for high yield of amylase and application thereof | |
| CN103320337B (en) | Lactobacillus pentosus | |
| CN106434779B (en) | Lactobacillus pentosus SS6 is producing the application in γ-aminobutyric acid | |
| CN109112067B (en) | A method of utilizing microorganism during macro gene order-checking assisting sifting tobacco fermentation | |
| CN106167785B (en) | A kind of E. Faecium strains and its application | |
| CN116144510B (en) | Aureobasidium pullulans and application thereof in improving beta-carotene content by fermentation | |
| CN110607253B (en) | Streptococcus thermophilus and proliferation culture method and application thereof | |
| CN102839140B (en) | L-lactic acid producing strain separated and screened out of corn soaking water | |
| CN109055284B (en) | Marine acid-producing strain for wine brewing and application thereof | |
| CN113583913B (en) | Composite fermentation microbial inoculum for preparing feed acidifier and application thereof | |
| CN110747149A (en) | Salt-tolerant methanogenic archaea and application thereof | |
| CN107384879B (en) | A kind of fermentation manufacturing technique of recombinant yeast pichia pastoris bacterium production liver detoxification enzyme | |
| CN103114053B (en) | Produce Lactobacillus coryniformis Shaanxi subspecies and the application thereof of high optical purity D-lactic acid | |
| CN112592854B (en) | Fermentation medium of high-density lactobacillus bulgaricus, fermentation method and application | |
| CN109112175A (en) | A method of building microbial co culture system high yield Pleurotus tuber-regium exocellular polysaccharide | |
| CN103966139A (en) | Lactobacillus brevis capable of producing gamma-aminobutyric acid at high yield in Sichuan pickle vegetables | |
| CN111592999B (en) | Bacillus simplex and application thereof in producing low-temperature amylase |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20220713 Address after: No. 3510, 35 / F, building 1, No. 500, middle section of Tianfu Avenue, Chengdu hi tech Zone, China (Sichuan) pilot Free Trade Zone, Chengdu, Sichuan 610096 Patentee after: Sichuan qianyiding Technology Co.,Ltd. Address before: 510642 No. five, 483 mountain road, Guangzhou, Guangdong, Tianhe District Patentee before: SOUTH CHINA AGRICULTURAL University |
|
| TR01 | Transfer of patent right | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20220810 Address after: Room C001, 4th Floor (1-3), Building 5, International Enterprise Center, No. 1, Guanshan 2nd Road, Wuhan East Lake New Technology Development Zone, Wuhan City, Hubei Province 430079 Patentee after: Wuhan mihuan Health Industry Development Co.,Ltd. Address before: No. 3510, 35 / F, building 1, No. 500, middle section of Tianfu Avenue, Chengdu hi tech Zone, China (Sichuan) pilot Free Trade Zone, Chengdu, Sichuan 610096 Patentee before: Sichuan qianyiding Technology Co.,Ltd. |
|
| TR01 | Transfer of patent right |