CN113795592A - 双链核酸的原位检测及其相关方法 - Google Patents

双链核酸的原位检测及其相关方法 Download PDF

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Publication number
CN113795592A
CN113795592A CN202080033656.7A CN202080033656A CN113795592A CN 113795592 A CN113795592 A CN 113795592A CN 202080033656 A CN202080033656 A CN 202080033656A CN 113795592 A CN113795592 A CN 113795592A
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China
Prior art keywords
target
preamplifier
sample
nucleic acid
probe
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Pending
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CN202080033656.7A
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English (en)
Chinese (zh)
Inventor
X-J.马
F.汤德内维斯
C.托德罗夫
B.张
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Advanced Cell Diagnostics Inc
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Advanced Cell Diagnostics Inc
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Publication of CN113795592A publication Critical patent/CN113795592A/zh
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6841—In situ hybridisation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
    • C12Q1/6886—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material for cancer
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • Pathology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • General Physics & Mathematics (AREA)
  • Hospice & Palliative Care (AREA)
  • Oncology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
CN202080033656.7A 2019-03-12 2020-03-11 双链核酸的原位检测及其相关方法 Pending CN113795592A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201962817449P 2019-03-12 2019-03-12
US62/817,449 2019-03-12
PCT/US2020/022010 WO2020185846A1 (en) 2019-03-12 2020-03-11 Detection of double stranded nucleic acids in situ and methods related thereto

Publications (1)

Publication Number Publication Date
CN113795592A true CN113795592A (zh) 2021-12-14

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CN202080033656.7A Pending CN113795592A (zh) 2019-03-12 2020-03-11 双链核酸的原位检测及其相关方法

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US (1) US20220177953A1 (https=)
EP (1) EP3938535A4 (https=)
JP (1) JP2022525101A (https=)
CN (1) CN113795592A (https=)
WO (1) WO2020185846A1 (https=)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20230313171A1 (en) * 2020-09-18 2023-10-05 Advanced Cell Diagnostics, Inc. Methods and compositions for removal of rna

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6465175B2 (en) * 1997-09-04 2002-10-15 Bayer Corporation Oligonucleotide probes bearing quenchable fluorescent labels, and methods of use thereof
US20120004132A1 (en) * 2010-07-02 2012-01-05 Affymetrix, Inc. Detection of Nucleic Acids and Proteins
US20120003648A1 (en) * 2010-07-01 2012-01-05 Affymetrix, Inc. Signal Multiplexing and Signal Amplification
US20120052498A1 (en) * 2010-07-01 2012-03-01 Affymetrix, Inc. Detection of Nucleic Acids
US20160153033A1 (en) * 2013-03-14 2016-06-02 Affymetrix, Inc. Detection of Nucleic Acids
CN108368543A (zh) * 2015-10-12 2018-08-03 领先细胞医疗诊断有限公司 高噪声样品中原位检测核苷酸变体及相关的组合物和方法

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DK2500439T4 (da) * 2005-06-20 2017-11-13 Advanced Cell Diagnostics Inc Kits og produkter til detektering af nukleinsyrer i individuelle celler og til identifikation af sjældne celler fra store heterogene cellepopulationer
WO2015123565A1 (en) * 2014-02-14 2015-08-20 The General Hospital Corporation Methods for diagnosing igg4-related disease
WO2017168332A1 (en) * 2016-03-28 2017-10-05 Boreal Genomics, Inc. Linked duplex target capture

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6465175B2 (en) * 1997-09-04 2002-10-15 Bayer Corporation Oligonucleotide probes bearing quenchable fluorescent labels, and methods of use thereof
US20120003648A1 (en) * 2010-07-01 2012-01-05 Affymetrix, Inc. Signal Multiplexing and Signal Amplification
US20120052498A1 (en) * 2010-07-01 2012-03-01 Affymetrix, Inc. Detection of Nucleic Acids
US20120004132A1 (en) * 2010-07-02 2012-01-05 Affymetrix, Inc. Detection of Nucleic Acids and Proteins
US20160153033A1 (en) * 2013-03-14 2016-06-02 Affymetrix, Inc. Detection of Nucleic Acids
CN108368543A (zh) * 2015-10-12 2018-08-03 领先细胞医疗诊断有限公司 高噪声样品中原位检测核苷酸变体及相关的组合物和方法

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Publication number Publication date
EP3938535A1 (en) 2022-01-19
US20220177953A1 (en) 2022-06-09
JP2022525101A (ja) 2022-05-11
WO2020185846A1 (en) 2020-09-17
EP3938535A4 (en) 2022-12-28

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