EP3938535A4 - Detection of double stranded nucleic acids in situ and methods related thereto - Google Patents

Detection of double stranded nucleic acids in situ and methods related thereto Download PDF

Info

Publication number
EP3938535A4
EP3938535A4 EP20770887.6A EP20770887A EP3938535A4 EP 3938535 A4 EP3938535 A4 EP 3938535A4 EP 20770887 A EP20770887 A EP 20770887A EP 3938535 A4 EP3938535 A4 EP 3938535A4
Authority
EP
European Patent Office
Prior art keywords
situ
detection
nucleic acids
double stranded
stranded nucleic
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP20770887.6A
Other languages
German (de)
French (fr)
Other versions
EP3938535A1 (en
Inventor
Xiao-Jun Ma
Farzaneh TONDNEVIS
Courtney TODOROV
Bingqing ZHANG
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Advanced Cell Diagnostics Inc
Original Assignee
Advanced Cell Diagnostics Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Advanced Cell Diagnostics Inc filed Critical Advanced Cell Diagnostics Inc
Publication of EP3938535A1 publication Critical patent/EP3938535A1/en
Publication of EP3938535A4 publication Critical patent/EP3938535A4/en
Withdrawn legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6841—In situ hybridisation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
    • C12Q1/6886—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material for cancer
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • Pathology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Hospice & Palliative Care (AREA)
  • Oncology (AREA)
  • Biomedical Technology (AREA)
  • General Physics & Mathematics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
EP20770887.6A 2019-03-12 2020-03-11 Detection of double stranded nucleic acids in situ and methods related thereto Withdrawn EP3938535A4 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201962817449P 2019-03-12 2019-03-12
PCT/US2020/022010 WO2020185846A1 (en) 2019-03-12 2020-03-11 Detection of double stranded nucleic acids in situ and methods related thereto

Publications (2)

Publication Number Publication Date
EP3938535A1 EP3938535A1 (en) 2022-01-19
EP3938535A4 true EP3938535A4 (en) 2022-12-28

Family

ID=72427590

Family Applications (1)

Application Number Title Priority Date Filing Date
EP20770887.6A Withdrawn EP3938535A4 (en) 2019-03-12 2020-03-11 Detection of double stranded nucleic acids in situ and methods related thereto

Country Status (5)

Country Link
US (1) US20220177953A1 (en)
EP (1) EP3938535A4 (en)
JP (1) JP2022525101A (en)
CN (1) CN113795592A (en)
WO (1) WO2020185846A1 (en)

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6465175B2 (en) * 1997-09-04 2002-10-15 Bayer Corporation Oligonucleotide probes bearing quenchable fluorescent labels, and methods of use thereof
US20120004132A1 (en) * 2010-07-02 2012-01-05 Affymetrix, Inc. Detection of Nucleic Acids and Proteins
US20120003648A1 (en) * 2010-07-01 2012-01-05 Affymetrix, Inc. Signal Multiplexing and Signal Amplification
US20120052498A1 (en) * 2010-07-01 2012-03-01 Affymetrix, Inc. Detection of Nucleic Acids
WO2017066211A1 (en) * 2015-10-12 2017-04-20 Advanced Cell Diagnostics, Inc. In situ detection of nucleotide variants in high noise samples, and compositions and methods related thereto

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3042963A1 (en) * 2005-06-20 2016-07-13 Advanced Cell Diagnostics, Inc. Methods of detecting nucleic acids in individual cells and of identifying rare cells from large heterogeneous cell populations
US9273349B2 (en) * 2013-03-14 2016-03-01 Affymetrix, Inc. Detection of nucleic acids
WO2015123565A1 (en) * 2014-02-14 2015-08-20 The General Hospital Corporation Methods for diagnosing igg4-related disease
US10961568B2 (en) * 2016-03-28 2021-03-30 Boreal Genomics, Inc. Linked target capture

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6465175B2 (en) * 1997-09-04 2002-10-15 Bayer Corporation Oligonucleotide probes bearing quenchable fluorescent labels, and methods of use thereof
US20120003648A1 (en) * 2010-07-01 2012-01-05 Affymetrix, Inc. Signal Multiplexing and Signal Amplification
US20120052498A1 (en) * 2010-07-01 2012-03-01 Affymetrix, Inc. Detection of Nucleic Acids
US20120004132A1 (en) * 2010-07-02 2012-01-05 Affymetrix, Inc. Detection of Nucleic Acids and Proteins
WO2017066211A1 (en) * 2015-10-12 2017-04-20 Advanced Cell Diagnostics, Inc. In situ detection of nucleotide variants in high noise samples, and compositions and methods related thereto

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
DELEAGE CLAIRE ET AL: "Defining HIV and SIV Reservoirs in Lymphoid Tissues", PATHOGENS AND IMMUNITY, vol. 1, no. 1, 1 January 2016 (2016-01-01), pages 68 - 106, XP055982117, ISSN: 2469-2964, DOI: 10.20411/pai.v1i1.100 *
See also references of WO2020185846A1 *

Also Published As

Publication number Publication date
EP3938535A1 (en) 2022-01-19
JP2022525101A (en) 2022-05-11
CN113795592A (en) 2021-12-14
WO2020185846A1 (en) 2020-09-17
US20220177953A1 (en) 2022-06-09

Similar Documents

Publication Publication Date Title
EP3775268A4 (en) Method of in situ gene sequencing
IL289050A (en) Assays and methods for detection of nucleic acids
EP3538662A4 (en) Methods of producing amplified double stranded deoxyribonucleic acids and compositions and kits for use therein
EP3359695A4 (en) Methods and applications of gene fusion detection in cell-free dna analysis
EP3792364A4 (en) Method for detecting nucleic acid based on prokaryotic argonaute protein and application thereof
EP3886075A4 (en) Parking space detection method and device
EP3781709A4 (en) Systems and methods for determining tumor fraction in cell-free nucleic acid
EP3662079A4 (en) Methods of detecting modified and unmodified dna
EP3565563A4 (en) Nucleic acids and methods for genome editing
EP3871935A4 (en) Parking space detection method and apparatus
EP3607064A4 (en) Method and kit for targeted enrichment of nucleic acids
EP3735481A4 (en) Methods for fabricating high resolution dna array and its application in sequencing
EP4103748A4 (en) Methods and materials for assessing nucleic acids
EP3674419A4 (en) Probe and method applying the same for enriching target region in high-throughput sequencing
EP4029929A4 (en) Instrument and method for extracting and detecting nucleic acids
EP4069868A4 (en) Methods of detection and analysis of nucleic acid in neural-derived exosomes
EP3780664A4 (en) Terminal detection method and terminal
EP3841218B8 (en) Nucleic acid based detection methods
EP3724883A4 (en) Detection of deletions and copy number variations in dna sequences
EP3827095A4 (en) Cell-free dna damage analysis and its clinical applications
EP3583112A4 (en) Compositions and methods for sequencing nucleic acids
EP3798221A4 (en) Fluorescent probe and preparation method and use thereof
EP4055437A4 (en) System and method for displaying an object with depths
EP3571315A4 (en) Methods for identifying and using small rna predictors
EP3809619A4 (en) Beam state detection method and terminal

Legal Events

Date Code Title Description
STAA Information on the status of an ep patent application or granted ep patent

Free format text: STATUS: THE INTERNATIONAL PUBLICATION HAS BEEN MADE

PUAI Public reference made under article 153(3) epc to a published international application that has entered the european phase

Free format text: ORIGINAL CODE: 0009012

STAA Information on the status of an ep patent application or granted ep patent

Free format text: STATUS: REQUEST FOR EXAMINATION WAS MADE

17P Request for examination filed

Effective date: 20210804

AK Designated contracting states

Kind code of ref document: A1

Designated state(s): AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR

DAV Request for validation of the european patent (deleted)
DAX Request for extension of the european patent (deleted)
A4 Supplementary search report drawn up and despatched

Effective date: 20221129

RIC1 Information provided on ipc code assigned before grant

Ipc: C12Q 1/6876 20180101ALI20221123BHEP

Ipc: C12Q 1/6837 20180101ALI20221123BHEP

Ipc: C12Q 1/682 20180101AFI20221123BHEP

STAA Information on the status of an ep patent application or granted ep patent

Free format text: STATUS: THE APPLICATION HAS BEEN WITHDRAWN

18W Application withdrawn

Effective date: 20230509