CN113790933A - Temperature-controlled windproof and light-resistant static box and gas in-situ collection method - Google Patents
Temperature-controlled windproof and light-resistant static box and gas in-situ collection method Download PDFInfo
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- 230000003068 static effect Effects 0.000 title claims abstract description 55
- 238000000034 method Methods 0.000 title claims abstract description 24
- 238000011065 in-situ storage Methods 0.000 title claims abstract description 6
- 239000004065 semiconductor Substances 0.000 claims abstract description 56
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 51
- 238000010438 heat treatment Methods 0.000 claims abstract description 40
- 238000005070 sampling Methods 0.000 claims abstract description 28
- 239000005431 greenhouse gas Substances 0.000 claims abstract description 22
- 239000000523 sample Substances 0.000 claims abstract description 21
- 230000017525 heat dissipation Effects 0.000 claims abstract description 14
- 238000001816 cooling Methods 0.000 claims abstract description 6
- 239000007789 gas Substances 0.000 claims description 33
- 239000011521 glass Substances 0.000 claims description 16
- 239000002689 soil Substances 0.000 claims description 12
- 238000004321 preservation Methods 0.000 claims description 11
- 230000000694 effects Effects 0.000 claims description 8
- 239000006260 foam Substances 0.000 claims description 5
- 238000009413 insulation Methods 0.000 claims description 5
- 230000005855 radiation Effects 0.000 claims description 3
- 239000008399 tap water Substances 0.000 claims description 3
- 235000020679 tap water Nutrition 0.000 claims description 3
- 238000005057 refrigeration Methods 0.000 abstract 3
- 239000000498 cooling water Substances 0.000 abstract 1
- GQPLMRYTRLFLPF-UHFFFAOYSA-N Nitrous Oxide Chemical compound [O-][N+]#N GQPLMRYTRLFLPF-UHFFFAOYSA-N 0.000 description 14
- VNWKTOKETHGBQD-UHFFFAOYSA-N methane Chemical compound C VNWKTOKETHGBQD-UHFFFAOYSA-N 0.000 description 11
- 229910002092 carbon dioxide Inorganic materials 0.000 description 9
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 description 8
- 230000008569 process Effects 0.000 description 7
- 230000008859 change Effects 0.000 description 6
- 238000004817 gas chromatography Methods 0.000 description 4
- 238000005259 measurement Methods 0.000 description 4
- 239000000700 radioactive tracer Substances 0.000 description 4
- 238000011161 development Methods 0.000 description 3
- 238000010586 diagram Methods 0.000 description 3
- 239000002803 fossil fuel Substances 0.000 description 3
- 238000004519 manufacturing process Methods 0.000 description 3
- 238000012544 monitoring process Methods 0.000 description 3
- 238000011160 research Methods 0.000 description 3
- 239000001569 carbon dioxide Substances 0.000 description 2
- 239000003337 fertilizer Substances 0.000 description 2
- 238000002347 injection Methods 0.000 description 2
- 239000007924 injection Substances 0.000 description 2
- 244000005700 microbiome Species 0.000 description 2
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- 230000004048 modification Effects 0.000 description 2
- 239000001272 nitrous oxide Substances 0.000 description 2
- 230000001360 synchronised effect Effects 0.000 description 2
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 240000007594 Oryza sativa Species 0.000 description 1
- 235000007164 Oryza sativa Nutrition 0.000 description 1
- 229910018503 SF6 Inorganic materials 0.000 description 1
- 108010074506 Transfer Factor Proteins 0.000 description 1
- 239000010868 animal carcass Substances 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 229910052799 carbon Inorganic materials 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 230000005264 electron capture Effects 0.000 description 1
- GPRLSGONYQIRFK-UHFFFAOYSA-N hydron Chemical compound [H+] GPRLSGONYQIRFK-UHFFFAOYSA-N 0.000 description 1
- 238000009434 installation Methods 0.000 description 1
- 239000000618 nitrogen fertilizer Substances 0.000 description 1
- 230000009935 nitrosation Effects 0.000 description 1
- 238000007034 nitrosation reaction Methods 0.000 description 1
- 230000001590 oxidative effect Effects 0.000 description 1
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- 238000010672 photosynthesis Methods 0.000 description 1
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- 235000009566 rice Nutrition 0.000 description 1
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- SFZCNBIFKDRMGX-UHFFFAOYSA-N sulfur hexafluoride Chemical compound FS(F)(F)(F)(F)F SFZCNBIFKDRMGX-UHFFFAOYSA-N 0.000 description 1
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/02—Devices for withdrawing samples
- G01N1/22—Devices for withdrawing samples in the gaseous state
- G01N1/2226—Sampling from a closed space, e.g. food package, head space
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L1/00—Enclosures; Chambers
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- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L7/00—Heating or cooling apparatus; Heat insulating devices
- B01L7/02—Water baths; Sand baths; Air baths
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/02—Devices for withdrawing samples
- G01N1/22—Devices for withdrawing samples in the gaseous state
- G01N2001/2282—Devices for withdrawing samples in the gaseous state with cooling means
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Abstract
The invention discloses a temperature-controlled windproof and light-proof static box and a greenhouse gas in-situ collection method. The static tank is divided into a lower base and an upper tank portion. The basin is fixed in on the base through pasting, the box is placed fixedly on the basin, semiconductor heating piece is fixed in the inside downside on one side of box, semiconductor refrigeration piece is fixed in the inside upside on one side of box, the cooling end of semiconductor heating piece is connected to holding water box, the heat dissipation end of semiconductor refrigeration piece is connected to the cooling water box, semiconductor heating piece, the refrigeration piece, the fan is connected with steady voltage direct current power supply through the power cord, steady voltage direct current power supply is connected with the temperature control instrument, the top cap is fixed with the box bonding, high sensitive temperature probe is connected with the temperature control instrument through top cap temperature probe jack, gaseous collection bag is fixed in the top cap through the sampling pipe, fixed rope is connected at the middle part of the box outside. The invention has the advantages of simple structure, easy operation, strong adaptability and capability of adjusting the internal temperature of the box body.
Description
Technical Field
The invention belongs to the field of global greenhouse gas emission research, mainly relates to a temperature-controlled windproof and light-resistant static box, and also relates to a greenhouse gas collection method. The method is suitable for greenhouse gas emission research of various land systems.
Background
In the context of global warming, the emission of greenhouse gases has attracted widespread attention from both domestic and foreign researchers. Studies have shown that global temperatures may rise by 1.1-6.4 ℃ at the end of this century. The six major types of greenhouse gases currently under control are: carbon dioxide, methane, nitrous oxide, hydrofluorocarbons, perfluorocarbons, and sulfur hexafluoride. Among them, carbon dioxide, methane, nitrous oxide are listed as three major greenhouse gases of interest worldwide. 2016 global CO2The concentration reaches 403.3ppm, which is improved by 0.75% compared with 2015 and 145% compared with 1750 years (before global industrialization). CO 22The production of the fertilizer is mainly generated by burning a large amount of fossil fuels through industrialized development, not protecting trees and trees, gargling and cutting, and oxidizing residual crops, animal carcasses and organic fertilizers in agriculture by various microorganisms in soil under different conditions. 2016 CH4The concentration reaches the new high of the current year, which is about 1853ppb, and is improved by 257 percent compared with 1750. CH in the atmosphere4Mainly comes from the exploitation of fossil fuel, methanogen or organism putrefaction generation in natural anoxic environment, and its greenhouse effect is CO220-25 times of. Research has shown that farms, landfills and fossil fuel development sites are CH4Is an important source of emissions. 2016N2The O is 328.99ppb, which is improved by 122 percent compared with 1750. N is a radical of2The production of O is mainly generated by applying excessive nitrogen fertilizer to farmland and transforming through the nitrification and nitrosation of microorganisms. In addition, with societyThe continuous development, the excessive discharge of industrial and agricultural wastewater leads to the shortage of carbon source in water, thereby influencing the efficiency of biological denitrification to generate N2O，N2The climate harm of O at least exceeds that of CO2300 times. The land ecosystem is one of important emission sources of greenhouse gases, and in order to measure and calculate the emission amount of greenhouse gases in the land ecosystem and evaluate the possible influence on the ecological environment, it is necessary to detect the content of greenhouse gases emitted by the land ecosystem.
Microweather method, isotope gas tracer method and static box-gas chromatography are the mainstream greenhouse gas monitoring method at present. The microclimate method is suitable for wide-range open sources, and has the advantages of strong precision, accurate measurement result, and no influence on the measurement process caused by the animal habits in the soil, the air condition and the soil under natural conditions. But a large number of precise instruments are needed, the cost is high, the operation is complex, and the requirements on the stability of the wind speed and the wind direction in the air environment are high. The principle of the tracer method is to use a reflective nuclide or a stable nuclide as a tracer to study the process of greenhouse gas emission, a large amount of tracer gas is needed during measurement, and the measurement cost is high.
The basic principle of static box-gas chromatography is to cover the measured ground surface with a bottomless box body, cover the box body on a base during sampling, collect the air in the box body through an injector at corresponding time intervals according to the experimental purpose, and measure the concentration of greenhouse gases through a gas chromatography instrument. The common shape is square or round, and the coverage area is usually 0.5-1m2The height is determined according to the height of vegetation on the ground (such as grassland, rice and trees). The static box-gas chromatography has the advantages of convenient carrying, simple operation and multipoint synchronous detection, and is widely used for monitoring the gas emission of the land ecosystem. But also has certain disadvantages under the condition of actual field environment operation. For example, in the field, the change of wind direction and wind force can cause the overturning of the box body, and the accuracy of synchronous multipoint sampling is influenced. In addition, the outdoor environment is complex, for example, direct sunlight and wind and rain can cause the temperature change, the difference between the internal temperature and the actual temperature of the closed box body can be caused, and the change of the temperature not only can influence the photosynthesis of plantsAction and respiration can also lead to an increase in the water content in the tank, further affecting the air pressure in the tank, so that the monitoring error increases. Therefore, there is a need to develop a gas static chamber with stable and automatic temperature control according to the external temperature and a feasible in-situ sampling method thereof.
Disclosure of Invention
Aiming at the problems, the invention provides a temperature control type windproof and light-resistant static box, which effectively avoids the temperature difference in the box body, reduces the error possibly generated in the gas collection process as much as possible, and can keep the stability of the box body in the field environment. The device has the advantages of obvious effect, simple structure and low manufacturing cost.
The other purpose of the invention is to keep the stability of the box body, effectively reduce the possibility of shaking and overturning caused by large wind area of the box body due to wind power change through the water tank screws and the fixing device of the supporting rod outside the box body, and further ensure the accuracy in the collecting process.
In order to achieve the above object, the present invention adopts the following measures:
a temperature control type windproof and light-resistant static box comprises a static box bottom (1), a water tank (2) and a box body (10); the bottom side in the box body (10) is provided with a semiconductor heating sheet (11-1), the front end of the semiconductor heating sheet (11-1) is connected with a heating sheet heat-conducting block (11-2) and a small fan (12) to ensure the heat dissipation effect, and the heating sheet cold-conducting block (11-3) is connected with a heat-insulating water tank (13); the upper end in the other side of the box body is connected with a semiconductor refrigerating sheet (4-1), the front end of the semiconductor refrigerating sheet (4-1) is connected with a cold guide block (4-2) and a small fan (12) to ensure the refrigerating effect, and the refrigerating sheet cold guide block (4-3) is connected with a heat radiation water tank (6); the outer wall of the box body (10) is wrapped with a reflective film foam heat insulation layer; the semiconductor heating sheet (11) and the refrigerating sheet (4) are connected with a temperature controller (8) through wires; the top of the static box is provided with a hole, a high-sensitivity thermometer probe (5) is arranged and is connected with a temperature controller (8) through a wire, and a voltage-stabilizing direct-current power supply (7) is connected with the temperature controller (8) and supplies power for the temperature controller; a vertical needle-shaped opening is reserved at the top, and a gas-tight needle is selected for sampling or a gas sampling valve and a gas collecting bag are connected according to the volume of the collected gas, so that automatic gas sampling is realized; static bottom of the case portion (1) is buried in the soil region to be measured, places basin (2) of pouring into clear water, and screw knob (14) through basin (2) limit fixedly connects upper box (10), inserts box outside support bar (3) on box (10) in the soil, accomplishes the fixed of basin (2) and box (10).
The semiconductor heating sheet (11) and the refrigerating sheet (4) are connected with a temperature controller (8) through wires; the top of the static box is provided with a hole, a high-sensitivity thermometer probe (5) is placed and connected with a temperature controller (8) through a wire, and a voltage-stabilizing direct-current power supply (7) is connected with the temperature controller (8) and supplies power to the temperature controller; a vertical needle-shaped opening is reserved at the top, and a gas-tight needle can be selected for sampling or a gas collecting valve and a gas collecting bag can be connected according to the volume of collected gas, so that automatic gas sampling is realized. The opening is internally provided with a replaceable airtight needle sample injection pad, so that the sampling airtightness is further ensured.
Static bottom of the case portion (1) is buried in the soil region to be measured, places basin (2) of pouring into clear water, and screw knob (14) through basin (2) limit fixedly connects upper box (10), inserts box outside support bar (3) on box (10) in the soil, accomplishes the fixed of basin (2) and box (10).
Preferred conditions are as follows:
the static box base is made of organic glass, and the ratio of the diameter to the height is 3: 1.8.
The length ratio of the outer diameter to the inner diameter of the water tank of the static tank is 4.5:3.5, and the static tank is formed by bonding organic glass plates.
The static box body is made of organic glass, the height and diameter ratio is 5.5:3.8, the box body top cover is made of organic glass, and the ratio of the diameter of the box body top cover to the diameter of the box body is 3.8: 4.2.
The model of the semiconductor heating piece and the model of the semiconductor refrigerating piece are TCE 1-12706.
The length-width ratio of the heat conducting block to the cold conducting block is 4:6, and the length-width-height ratio of the specification of the fan at the upper end is 4:4: 10.
The length-width-height ratio of the specifications of the heat preservation water tank and the heat dissipation water tank is 100:120: 53.
The top cover of the box body (10) is provided with a sampling tube and a probe hole which can be connected with a high-sensitivity temperature probe (5) and a temperature control instrument (8).
The outer wall of the box body (10) is wrapped with a reflective film foam heat insulation layer.
A gas in-situ collection method for a temperature-controlled windproof and light-resistant static box comprises the following steps:
a. horizontally inserting the base into the ground;
b. pouring tap water into a base water tank, and covering a box body to keep the box body sealed;
c. connecting a temperature controller with a mobile power supply, measuring the temperature in the box, setting the starting temperature of the temperature controller to be higher than the air temperature by 0.5 ℃, and setting the stopping temperature to be lower than the air temperature by 0.5 ℃;
d. connecting the semiconductor refrigerating sheet and the semiconductor heating sheet fixed on two sides of the box body to a temperature controller, and injecting water into the heat dissipation water tank and the heat preservation water tank;
e. the time, the quantity and the times of collecting samples are set through a sampling hole at the upper end of the box body;
f. injecting the collected sample into a gas chromatograph for detecting greenhouse gas (CO)2,CH4,N2O), the discharge rate and the discharge weight per unit area were calculated.
The static tank of the present invention is divided into a lower base and an upper tank portion. The water tank is fixed on the base by sticking, the box body is placed on the water tank and is screwed and fixed by a screw knob, the semiconductor heating sheet is fixed on one lower side inside the box body, the semiconductor refrigerating sheet is fixed on one upper side inside the box body, the heat preservation water tank is fixed on one lower side outside the box body and is connected with the refrigerating end of the semiconductor heating sheet, the heat dissipation water tank is fixed on one upper side outside the box body and is connected with the radiating end of the semiconductor refrigerating sheet, the micro fan is respectively connected on the heat conducting block of the heating sheet and the refrigerating block of the semiconductor refrigerating sheet, the semiconductor heating sheet and the refrigerating sheet, the fan passes through the power cord and is connected with steady voltage DC power supply, and steady voltage DC power supply is connected with the temperature control instrument, and the top cap bonds fixedly with the box, and high sensitive temperature probe is connected with the temperature control instrument through top cap temperature probe jack, and gas acquisition bag is fixed in the top cap through the sampling pipe, and fixed rope is connected at box outside middle part. The invention has the advantages of simple structure, easy operation, strong adaptability and capability of adjusting the internal temperature of the box body.
Compared with the prior art, the invention has the following beneficial effects:
the invention can effectively control the temperature in the box body, and the purposes of temperature rise and temperature reduction are realized through the semiconductor heating sheet and the semiconductor refrigerating sheet. In the sampling process, the internal temperature of the static box body is automatically adjusted, so that the temperature difference between the inside and the outside of the box body is avoided, and the error of the terrestrial low ecosystem in the greenhouse gas emission process is further reduced.
The invention can realize accurate temperature control through the temperature control system, and the temperature difference between the inside and the outside of the box body is not more than 0.2 ℃.
The invention has simple installation, the sampling hole reserved on the top can select an automatic sampler or a manual airtight needle for sampling according to the experimental requirements, and the operability is strong.
According to the invention, through the fixing device of the water tank screw and the supporting rod outside the box body, the possibility of shaking and overturning caused by large wind area of the box body due to wind power change is effectively reduced, and the accuracy in the collection process is further ensured.
Drawings
FIG. 1 is a block diagram of the present invention patent. The details are shown in the attached drawings of the specification.
Fig. 2 is a structure diagram of the semiconductor cooling plate of the invention.
Wherein: the device comprises a static box base 1, a water tank 2, a support bar on the outer side of a box body 3, a semiconductor refrigerating sheet 4-1, a refrigerating sheet cooling guide block 4-2, a refrigerating sheet heat guide block 4-3, an electric wire 4-4, a high-sensitivity temperature probe 5, a heat dissipation water tank 6, a voltage-stabilizing direct-current power supply 7, a temperature control instrument 8, a gas collection bag 9, a box body 10, a semiconductor heating sheet 11-1, a heating sheet heat guide block 11-2, a heating sheet cooling guide block 11-3, a fan 12, a heat preservation water tank 13 and a screw knob 14.
Detailed Description
The schematic diagram of the test device used is shown in fig. 1, and the embodiment of the invention is explained by combining fig. 1 and fig. 2:
a connection mode of a temperature-control windproof and light-resistant static box is as follows: static bottom of the case portion (1) buries in the soil region that awaits measuring, and clear water is poured into in basin (2), fixes top box (10) through screw knob (14) on basin (2) limit, inserts in the soil with box outside support bar (3) on box (10), accomplishes the fixed work of basin (2) and box (10). The outer wall of the static box body (10) is wrapped by a reflective film foam heat insulation layer, the bottom of one side of the box body is connected with a semiconductor heating sheet (11-1), the front end of the semiconductor heating sheet (11-1) is connected with a heating sheet heat conduction block (11-2) and a small fan (12) to ensure the heat dissipation effect, and the heating sheet cold guide block (11-3) is connected with a heat preservation water tank (13). The upper end of one side of the box body is connected with a semiconductor refrigerating sheet (4-1), the front end of the semiconductor refrigerating sheet (4-1) is connected with a cold guide block (4-2) and a small fan (12) to ensure the refrigerating effect, and the cold guide block (4-3) of the refrigerating sheet is connected with a heat radiation water tank (6). The semiconductor heating sheet (11-1) and the refrigerating sheet (4-1) are connected with a temperature controller (8) through wires (4-4). Static case top trompil is placed high sensitivity thermometer probe (5) and is connected with temperature controller (8) through the electric wire, and steady voltage DC power supply (7) are connected with temperature controller (8), supply power for temperature controller. A vertical needle-shaped opening is reserved at the top, and a gas-tight needle (less than 10mL) can be selected for sampling or a gas collecting valve and a gas collecting bag (more than 10mL) can be connected according to the volume of collected gas, so that automatic gas sampling is realized. The opening is internally provided with a replaceable airtight needle sample injection pad, so that the sampling airtightness is further ensured.
The static box base is made of organic glass, and the ratio of the diameter to the height is 3: 1.8.
The length ratio of the outer diameter to the inner diameter of the water tank of the static tank is 4.5:3.5, and the static tank is formed by bonding organic glass plates.
The static box body is made of organic glass, the height and diameter ratio is 5.5:3.8, the box body top cover is made of organic glass, and the ratio of the diameter of the box body top cover to the diameter of the box body is 3.8: 4.2.
The model of the semiconductor heating piece and the model of the semiconductor refrigerating piece are TCE 1-12706.
The length-width ratio of the heat conducting block to the cold conducting block is 4:6, and the length-width-height ratio of the specification of the fan at the upper end is 4:4: 10.
The length-width-height ratio of the specifications of the heat preservation water tank and the heat dissipation water tank is 100:120: 53.
The top cover of the box body (10) is provided with a sampling tube and a probe hole which can be connected with a high-sensitivity temperature probe (5) and a temperature control instrument (8).
The outer wall of the box body (10) is wrapped with a reflective film foam heat insulation layer.
A sampling step of a temperature-controlled windproof and light-resistant static box is as follows:
a. the base is horizontally inserted into the ground.
b. Pouring tap water into the base water tank, covering the tank body, and keeping the tank body sealed.
c. Connecting the temperature controller with a mobile power supply, measuring the temperature in the box, setting the starting temperature of the temperature controller to be 0.5 ℃ higher than the air temperature, and setting the stopping temperature to be 0.5 ℃ lower than the air temperature.
d. And connecting the semiconductor refrigerating sheets and the semiconductor heating sheets fixed on the two sides of the box body to a temperature controller, and injecting water into the heat dissipation water tank and the heat preservation water tank.
e. Samples were collected every 30 minutes through a sampling hole in the upper end of the box, 10ml each time, three times each time.
f. And after 4-6 times of gas sample collection, the power supply is cut off, and the device is arranged.
g. Injecting the collected sample into a gas chromatograph for detecting greenhouse gas (CO)2,CH4,N2O) and the emission rate and the emission weight per unit area of the greenhouse gas were calculated according to the following formulas.
Example 1:
in the artificial climate box, the humidity of the artificial climate box is kept unchanged, the semiconductor heating sheet (11-1) and the semiconductor refrigerating sheet (4-1) are connected to a temperature controller (8) through wires (4-4), the other end of the temperature controller (8) is connected with a high-sensitivity temperature probe (5) and is inserted into the box body from an opening of a top cover of the box body (10), and the temperature controller (8) is connected with a voltage-stabilizing direct-current power supply (7) through an electric wire, when the temperature in the box body (10) is higher than the air temperature by 0.1 ℃, the semiconductor refrigerating sheet (4-1) starts to work, when the temperature in the box body is lower than the air temperature by 0.1 ℃, the semiconductor heating sheet (11-1) starts to work, when the temperature in the box body is consistent with the air temperature, the temperature controller (8) stops working to cut off the power supply of the semiconductor heating sheet (11-1) and the semiconductor refrigerating sheet (4-1). The temperature of the artificial climate box is set to 7 ℃ from 31.7 ℃ of room temperature, only 15 minutes are needed to find that the temperature in the box body is reduced to 7 ℃ from 31.7 ℃, the temperature of the constant temperature box is adjusted to 0 ℃ in order to verify the refrigerating capacity of the semiconductor refrigerating sheet, and the temperature in the box body can reach 5.8 ℃ after one hour of electrification. The temperature of the incubator was set to 40 ℃ from 26 ℃ which was the room temperature, and it was found that the semiconductor heating sheet (11-1) was able to maintain the temperature in the incubator at 40 ℃ stably for only 5 minutes. The static box base is made of 1.2cm of organic glass, the diameter of the static box base is 30cm, and the length of the static box base is 18 cm. The water tank is formed by bonding organic glass plates with the outer diameter of 45cm, the inner diameter of 35cm and the thickness of 12 cm. The box is made by the organic glass that thickness is 3mm, and the height is 55cm, and the diameter is 38cm, and the box top cap is made by the organic glass that thickness is 3mm, and the radius is 42 cm. The specifications of the semiconductor heating sheet and the semiconductor refrigerating sheet are 40 × 3.9mm in length, 40 × 10mm in width, the specifications of the fan are 40 × 10mm in length, the specifications of the heat conducting block and the cold conducting block are 60mm in length, and the specifications of the heat preservation water tank and the heat dissipation water tank are 100 × 120 × 53mm in length. The heating system and the refrigerating system have the power of 46W, the small fan is 1.5W and the whole power is about 100W under the voltage of 12V.
Example 2:
the temperature control type windproof and light-resistant static box is installed in actual soil, a sampling hole in the upper end of the box body is used for manually sampling through an airtight needle, and samples are collected every 30 minutes, 10ml each time and three times each time. After 6 gas samples were collected, the power was turned off. Injecting the collected sample into Fuli GC9790Plus gas chromatograph to detect greenhouse gas (CO)2,CH4,N2O)，CO2,CH4By hydrogen ion flame detector (FID), N2O is detected by Electron Capture Detector (ECD), column box temperature: 60 ℃ (6.5 min for holding, 160 ℃ for holding at 20 ℃/min for holding 5min, 16.5min in total), FID 250 ℃, ECD 250 ℃, and the split ratio of 5: 1, the greenhouse gas content of the three components can be calculated. And then according to the discharge rate of greenhouse gases (F1) and the discharge rate per unit area (F2).
It can be calculated as follows:
greenhouse gas emission rate:
F1＝(V/A)×(d Ct/dt)+(Vg/A)×(Ct-Ca)+Vd×Ct
f is CO2,CH4,N2Flux of O (g.m)-2·s-1) Ct is the concentration (g.m) at time t-3) Ca is CO in the surrounding environment2,CH4,N2Concentration of O (g.m)-3) T is the length of time(s) the tank is closed, V is the tank volume (m)3) And A is the area (m) covered by the box2) Vg is the rate of gas leakage from the tank (m)3·s-1)。VdIs CO2,CH4,N2Deposition rate (m.s) of O on the surface of box, plant and soil-1)。
Discharge rate per unit area:
F2＝k×(273/T)×(V/A)×(Δc/Δt)
f is CO2,CH4,N2Flux of O (ug m)-2·h-1) K is a transfer factor, equal to N1.25ug uL-1(ii) a T is the in-tank temperature (open), V is the tank volume (L), A is the coverage area (m)2) Δ c/Δ t is the change in concentration in the tank (uL. L)-1·h-1)。
While the methods and techniques of the present invention have been described in terms of preferred embodiments, it will be apparent to those of ordinary skill in the art that variations and/or modifications of the methods and techniques described herein may be made without departing from the spirit and scope of the invention. It is expressly intended that all such similar substitutes and modifications apparent to those skilled in the art are deemed to be within the spirit, scope and content of the invention. The invention belongs to the known technology.
Claims (8)
1. A temperature control type windproof and light-resistant static box; the device is characterized by comprising a static box bottom (1), a water tank (2) and a box body (10); the bottom side in the box body (10) is provided with a semiconductor heating sheet (11-1), the front end of the semiconductor heating sheet (11-1) is connected with a heating sheet heat-conducting block (11-2) and a small fan (12) to ensure the heat dissipation effect, and the heating sheet cold-conducting block (11-3) is connected with a heat-insulating water tank (13); the upper end in the other side of the box body is connected with a semiconductor refrigerating sheet (4-1), the front end of the semiconductor refrigerating sheet (4-1) is connected with a cold guide block (4-2) and a small fan (12) to ensure the refrigerating effect, and the refrigerating sheet cold guide block (4-3) is connected with a heat radiation water tank (6); the outer wall of the box body (10) is wrapped with a reflective film foam heat insulation layer; the semiconductor heating sheet (11) and the refrigerating sheet (4) are connected with a temperature controller (8) through wires; the top of the static box is provided with a hole, a high-sensitivity thermometer probe (5) is arranged and is connected with a temperature controller (8) through a wire, and a voltage-stabilizing direct-current power supply (7) is connected with the temperature controller (8) and supplies power for the temperature controller; a vertical needle-shaped opening is reserved at the top, and a gas-tight needle is selected for sampling or a gas sampling valve and a gas collecting bag are connected according to the volume of the collected gas, so that automatic gas sampling is realized; static bottom of the case portion (1) is buried in the soil region to be measured, places basin (2) of pouring into clear water, and screw knob (14) through basin (2) limit fixedly connects upper box (10), inserts box outside support bar (3) on box (10) in the soil, accomplishes the fixed of basin (2) and box (10).
2. The temperature controlled wind and light resistant static case of claim 1; the static box is characterized in that a base of the static box is made of organic glass, and the ratio of the diameter to the height of the base is 3: 1.8.
3. The temperature controlled wind and light resistant static case of claim 1; the static tank is characterized in that the length ratio of the outer diameter to the inner diameter of the static tank water tank is 4.5:3.5, and the static tank water tank is formed by bonding organic glass plates.
4. The temperature controlled wind and light resistant static case of claim 1; the static box is characterized in that the box body of the static box is made of organic glass, the height-diameter ratio is 5.5:3.8, the top cover of the box body is made of organic glass, and the ratio of the diameter of the top cover of the box body to the diameter of the box body is 3.8: 4.2.
5. The temperature controlled wind and light resistant static case of claim 1; the semiconductor heating plate and the semiconductor cooling plate are characterized in that the model number of the semiconductor heating plate and the model number of the semiconductor cooling plate are TCE 1-12706.
6. The temperature controlled wind and light resistant static case of claim 1; the fan is characterized in that the length-width ratio of the heat conducting block to the cold conducting block is 4:6, and the length-width-height ratio of the specification of the fan at the upper end is 4:4: 10.
7. The temperature controlled wind and light resistant static case of claim 1; the heat-preservation water tank and the heat-dissipation water tank are characterized in that the length-width-height ratio of the specifications of the heat-preservation water tank and the heat-dissipation water tank is 100:120: 53.
8. A gas in-situ collection method for a temperature-controlled windproof and light-resistant static box is characterized by comprising the following steps:
a. horizontally inserting the base into the ground;
b. pouring tap water into a base water tank, and covering a box body to keep the box body sealed;
c. connecting a temperature controller with a mobile power supply, measuring the temperature in the box, setting the starting temperature of the temperature controller to be higher than the air temperature by 0.5 ℃, and setting the stopping temperature to be lower than the air temperature by 0.5 ℃;
d. connecting the semiconductor refrigerating sheet and the semiconductor heating sheet fixed on two sides of the box body to a temperature controller, and injecting water into the heat dissipation water tank and the heat preservation water tank;
e. the time, the quantity and the times of collecting samples are set through a sampling hole at the upper end of the box body;
f. injecting the collected sample into a gas chromatograph for detecting greenhouse gas (CO)2,CH4,N2O), the discharge rate and the discharge weight per unit area were calculated.
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