CN108318607B - Temperature-controllable transparent static box and greenhouse gas field in-situ acquisition method - Google Patents
Temperature-controllable transparent static box and greenhouse gas field in-situ acquisition method Download PDFInfo
- Publication number
- CN108318607B CN108318607B CN201810097558.4A CN201810097558A CN108318607B CN 108318607 B CN108318607 B CN 108318607B CN 201810097558 A CN201810097558 A CN 201810097558A CN 108318607 B CN108318607 B CN 108318607B
- Authority
- CN
- China
- Prior art keywords
- temperature
- water tank
- pipe
- box body
- water
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Images
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/04—Preparation or injection of sample to be analysed
- G01N30/06—Preparation
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/02—Devices for withdrawing samples
- G01N1/22—Devices for withdrawing samples in the gaseous state
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Physics & Mathematics (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Engineering & Computer Science (AREA)
- Sampling And Sample Adjustment (AREA)
Abstract
The invention discloses a temperature-controllable transparent static box and a field in-situ greenhouse gas collecting method.A water tank is adhered and fixed on a soil ring, a refrigeration water tank is connected with a box body, the refrigeration water tank is fixed on the box body through a guide rail, a water pump is arranged at the bottom of the refrigeration water tank, a water inlet pipe is sleeved with a water outlet of the water pump, an air pressure balance pipe is communicated with the box body through an air pressure balance hole, one end of a water outlet pipe is arranged on the refrigeration water tank, a top cover is hermetically adhered with the top of the box body, the top of an air cooler is fixed with the inner side of the top cover, the output end of the air cooler is connected with the water pump through a water pump power supply controller, a high-sensitivity temperature probe is connected with a temperature controller through a temperature probe jack, a mobile power supply is directly connected with. The device has the advantages of obvious effect, low cost, simple structure, portability, easy operation, wide application range and high sensitivity of results, and can effectively control the temperature and the air pressure in the box body.
Description
Technical Field
The invention belongs to the field of global change ecology research, and particularly relates to a temperature-controllable transparent static box and an in-situ greenhouse gas sampling method. The method is suitable for researching greenhouse gas emission of a land ecosystem, and directly obtains net exchange rate (NEE) of greenhouse gas of the land ecosystem-atmosphere through direct measurement of net exchange of greenhouse gas between land and gas.
Background
CO2，CH4，N2O is the three major greenhouse gas in the world, 2016 worldwide CO2The concentration reaches 403.3ppm (1ppm is one part per million), is higher than 400ppm in 2015 and is 145 percent of the level before industrialization (1750 years); the methane concentration reaches the new high, about1853ppb (1ppb is parts per billion) which is 257% of the pre-industrial level; the nitrous oxide concentration was 328.9ppb, 122% of the pre-industrial level. The change of the atmospheric greenhouse gas concentration mainly depends on the fluctuation of carbon nitrogen flux among various carbon nitrogen libraries participating in carbon nitrogen cycle, and the research on the greenhouse gas flux change of the terrestrial ecosystem is one of the core contents of the global change research.
The current greenhouse gas flux monitoring method mainly comprises a microclimate vorticity correlation method and a static box-gas chromatography, wherein the microclimate vorticity correlation method is used for directly measuring the gas concentration change in the air at high frequency by measuring the three-dimensional wind speed and the air turbulence speed at a specific height above a certain Ecosystem type so as to obtain the Net Ecosystems Exchange (NEE) of the greenhouse gas of the Ecosystem. The method has the advantages of quick response, no damage to vegetation and capability of achieving the field in-situ continuous monitoring target, but the method has higher requirements on the uniformity and the gradient of the underlying surface, and is expensive in instrument and equipment, high in maintenance cost and difficult to popularize in a large area.
The basic working principle of static box-gas chromatography is to cover the surface to be measured with a closed bottomless box (made of chemically stable material) with known volume and bottom area, extract the gas in the box at intervals, measure the concentration of the target gas by a gas chromatograph, and then calculate the exchange rate of trace gas between the surface and the gas of the cover according to the change rate of the gas concentration along with the time. The method has the advantages of strong adaptability, simple structure and operation, low cost, high sensitivity and the like, and is widely applied to greenhouse gas CO of the typical land ecosystem of China2、CH4And N2And synchronously monitoring the O.
The technical problem of adopting the transparent box to measure the micro gas exchange of the ecological system is that the temperature of the air in the box rises quickly and is difficult to control in the measuring process. The sharp rise in temperature during sampling affects not only photosynthesis and respiration of plants, but also increases in the moisture content of the enclosure (particularly for rice and wetland ecosystems), increases in the air pressure of the enclosure, and affects CO2Measurement of crossover. Through Chinese patent web and related thesis website retrieval, the current situation is thatMost static box designs are basically static dark boxes (light tight boxes), such as "static boxes for collecting greenhouse gases from field crops", publication no: CN206095725U, "a static case system of wetland carbon emission is observed in situ", publication no: CN 203572823U. Although the static dark box can relieve the too fast rise of the temperature in the box to a certain extent; however, the static dark box can only be used for measuring the breathing of the ecosystem, and cannot directly measure the NEE of the terrestrial ecosystem, and the NEE of the ecosystem can only be indirectly estimated through the breathing of a soil-plant system and has large errors. Therefore, there is a need to invent a temperature-controllable transparent static box and a method for in-situ sampling of greenhouse gases thereof for directly determining the NEE of the ecosystem.
Disclosure of Invention
The invention aims to provide a temperature-controllable transparent static box, which has the advantages of obvious effect, low cost, simple structure, portability, easy operation and easy popularization and can effectively control the temperature and the air pressure in the box body.
Another objective of the invention is to provide a method for collecting greenhouse gases in situ in the field, which has wide application range and high result sensitivity and can meet the requirement of directly measuring net exchange of greenhouse gases (NEE) of an ecosystem.
In order to achieve the purpose, the invention adopts the following technical measures:
a temperature-controllable transparent static box comprises a base, a box body, a top cover, a temperature control device, a gas production device and an air pressure balance pipe. The method is characterized in that: the soil ring is buried in a sample plot to be tested, the water tank is bonded and fixed on the soil ring, and the refrigeration water tank is connected with the box body; the box body is placed in a water tank filled with tap water; the refrigeration water tank is fixed on the side surface of the box body through a guide rail; the water pump is arranged at the bottom of the refrigerating water tank, and the cooling liquid is directly poured into the refrigerating water tank; the water inlet pipe is sleeved with the water outlet of the water pump, and the other end of the water inlet pipe is sleeved with the water inlet at the bottom of the air cooler through the water inlet hole; the air pressure balance pipe is communicated with the box body through an air pressure balance hole; one end of the water outlet pipe is placed on the upper edge of the refrigeration water tank, and the other end of the water outlet pipe is sleeved with a water outlet at the upper part of the air cooler through a water outlet hole; the top cover is hermetically bonded with the top of the box body; the top of the air cooler is fixed with the inner side of the top cover; the fan is fixed at the bottom of the air cooler; the temperature controller is fixed on the outer surface of the top cover, and the output end of the temperature controller is connected with the water pump through the water pump power supply controller; the high-sensitivity temperature probe is connected with the temperature controller through the temperature probe jack; the mobile power supply is directly connected with the input end of the temperature controller and the fan through a fan power supply hole; one end of the sampling pipe is communicated with the box body through a sampling hole; the gas production valve is fixed at the upper end of the sampling pipe; the waterproof cover is fixed on the surface of the top cover through an aluminum alloy hinge and covers the temperature controller, the water pump power supply controller and the mobile power supply.
Through the technical measures of the components, the transparent static box with the controllable temperature is obtained.
The key parts of the static tank are an air cooling circulation system which comprises an air cooler, a refrigeration water tank and a water pump. Through the operation of the system, the problem that the temperature in the box rises too fast in the sampling process of the current transparent static box is solved. Compared with the prior art, the invention mainly progresses as follows: the temperature in the box body is controlled in the sampling process, so that the measurement error of an ecosystem NEE caused by temperature rise is avoided; the main difference between the technical scheme and the prior art is as follows: according to the technical scheme, the sampling box air cooling system and the temperature control system are added, so that the accuracy of the measuring result is ensured.
The soil ring and the water tank form a base, and the base is formed by bonding a PVC pipe with the pipe diameter of 390-410 mm and a PVC plate with the thickness of 15-17 mm; the box body is made of an organic glass tube with the wall thickness of 4-6 mm, the tube diameter of 390-410 mm and the height of 590-610 mm; the top cover is made of an organic glass plate with the thickness of 4-6 mm and the diameter of 400-420 mm; the top cover and the box body are firmly bonded through organic glass glue. The temperature control device consists of an air cooler, a refrigeration water tank, a multi-channel temperature controller with the control voltage of 12V, a fan with the input voltage of 12V and the diameter of 140mm, a water pump with the input voltage of 12V and the water outlet diameter of 5-7 mm, a water inlet pipe with the inner diameter of 5-7 mm and the outer diameter of 7-9 mm, and a water outlet pipe. The air cooler is made by winding a red copper pipe with the pipe diameter of 7-9 mm, the wall thickness of 0.4-0.6 mm and the length of 1.8-2.2 m into a cylindrical copper disc with the diameter of 140-160 mm, the cooling water tank is made of an organic glass pipe with the pipe diameter of 90-110 mm and the height of 140-160 mm, and the surface of the cooling water tank is wrapped with heat insulation tinfoil paper and heat insulation foam to achieve the purpose of heat insulation. The assembly mode of the temperature control device is that one side of the air cooler is fixed with the center of the inner side of the top cover, and then the fan is fixed with the lower surface of the air cooler and keeps the center aligned. The lower end of the air cooler is connected with a water inlet pipe, the upper end of the air cooler is connected with a water outlet pipe, the water inlet pipe and the water outlet pipe are connected with a refrigeration water tank through a small hole (aperture is 8mm) in the wall of the tank body, and the refrigeration water tank is fixed through a guide rail arranged on the outer side of the tank wall. The water pump is arranged at the bottom of the refrigeration water tank, the water outlet of the water pump is connected with the water inlet pipe, and the water outlet pipe is naturally arranged in the refrigeration water tank. The water pump power line is connected with the output end of the temperature controller through a quick waterproof joint, and the fan power supply is connected with the input end of the temperature controller through a quick waterproof joint. The temperature controller is provided with a temperature probe which extends into the static tank through a small hole (aperture 4mm) of the top cover. The gas production device is formed by connecting a transparent silicone tube with the inner diameter of 5-7 mm and the outer diameter of 9-11 mm and a medical three-way valve, and the gas production device extends into the sampling box through a small hole (the aperture is 10mm) of the top cover. The air pressure balance pipe is formed by connecting a transparent capillary tube with the inner diameter of 1-3 mm, the outer diameter of 3-5 mm and the length of 90-110 mm with a water tank through a small hole (aperture of 4mm) in the tank wall, one end of the air pressure balance pipe is arranged in the sampling tank, and the other end of the air pressure balance pipe is naturally arranged below the liquid level of the refrigeration water tank. And after all the accessories are installed, coating glass cement on all the small holes for sealing.
An in-situ sampling method for greenhouse gases comprises the following steps:
step one, burying a sampling box base into a sampling area one day in advance, and keeping a base water tank horizontal;
placing the sampling box in a base water tank during sampling, and pouring a tap water sealing box body and a base into the water tank;
injecting cooling liquid (the servant cp1101) which is frozen at the temperature of 18 ℃ below zero for 11 to 13 hours into a refrigeration water tank (the freezing point is-25 ℃);
connecting a temperature controller (XH-W3001) with a 12V mobile power supply, and measuring the instant temperature in the box, namely the air temperature (-10-50 ℃);
step five, setting the starting temperature of the temperature controller to be air temperature (-5-40 ℃) plus 0.1 ℃ (namely, switching on the power supply of a water pump when the temperature in the box rises to 0.1 ℃), and setting the stopping temperature to be air temperature (-5-40 ℃) minus 0.1 ℃ (namely, switching off the power supply of the water pump when the temperature in the box is lower than the air temperature by 0.1 ℃);
step six, turning on a power switch of a water pump, and at the moment, when the temperature in the box is higher than the air temperature by 0.1 ℃, enabling a refrigerating water pump to work to circulate cooling liquid into an air cooling device (red copper tube) to achieve the purpose of quickly cooling; when the temperature in the refrigerator is lower than the air temperature by 0.1 ℃, the refrigeration water pump stops working, and the low-temperature (-18-0 ℃) cooling liquid in the cooling device flows back to the refrigeration water tank through the negative pressure of the water pump, so that the purpose of stopping cooling is achieved.
And seventhly, collecting the sample once every 5-10 minutes by using a gas sampling device, wherein 30ml of sample is collected every time, and the collection is carried out for 4-6 times.
And step eight, after the gas is collected for 4-6 times, closing a water pump switch, disconnecting a power supply, taking down the static tank, and recovering the cooling liquid.
And ninthly, analyzing the greenhouse gas concentration of the collected sample by a gas chromatograph (Agilent 7890A) as soon as possible, wherein the variation of the greenhouse gas concentration with time is the net exchange amount (NEE) of the greenhouse gas between the ecological system and the atmosphere.
Through the technical measures of the nine steps:
the method comprises the key steps of third, fourth, fifth and sixth, solves the technical problem of too fast temperature rise in the greenhouse gas field in-situ sampling box through the implementation of the key steps, and realizes the temperature control in the sampling box. Compared with the prior art, the technical scheme adds the air temperature cooling step in the gas collection process, thereby avoiding the influence on the air pressure in the box and the photosynthesis of plants due to the rise of the air temperature and ensuring the accuracy of the NEE measurement result of the ecosystem.
Compared with the prior art, the invention has the following beneficial effects:
(1) the invention can effectively control the temperature in the box body to rise too fast in transparent box sampling, and the temperature control precision in the box body can reach 0.1 ℃;
(2) the sampling method has no damage to the vegetation, directly measures the net exchange amount of greenhouse gases between an ecological system and the atmosphere, and effectively avoids the error of indirectly estimating NEE through the respiration of the ecological system;
(3) the invention has the advantages of rapid installation, simple and orderly operation, time and labor saving, low cost, strong mobility and simultaneous multi-point observation.
The invention is applied to the Dongting lake wetland under the support of the national natural science foundation project 'Dongting lake wetland soil carbon emission and the response mechanism thereof to the hydrological situation change', and the experimental result shows that: compared with the traditional sampling box, the device can effectively control the temperature in the sampling box to rise too fast; ecosystem NEE (about 13.6%) would be overestimated using traditional sample boxes.
TABLE 1 comparison of experimental results in sampling boxes
Drawings
FIG. 1 is a schematic diagram of a temperature-controllable transparent static chamber.
FIG. 2 is a top view of a temperature controllable transparent static tank top cover.
Wherein: 1-soil ring (PVC), 2-water tank, 3-box, 4-refrigeration water tank (common), 5-water pump (common), 6-cooling liquid (vehicle slave cp1101), 7-water inlet pipe, 8-air pressure balance pipe, 9-water outlet pipe, 10-water inlet hole, 11-air pressure balance hole, 12-water outlet hole, 13-top cover, 14-air cooler (copper pipe), 15-fan (common), 16-temperature controller (XH-W3001), 17-water pump power switch (common), 18-high sensitive temperature probe (TP-K01), 19-mobile power supply (common), 20-gas production pipe, 21-gas production valve (common), 22-waterproof cover, 23-sampling hole, 24-temperature probe jack, 25-fan power supply hole.
Detailed Description
Example 1:
according to the drawings 1 and 2, the temperature-controllable transparent static box is composed of a soil ring 1, a water tank 2, a box body 3, a refrigeration water tank 4, a water pump 5, cooling liquid 6, a water inlet pipe 7, an air pressure balance pipe 8, a water outlet pipe 9, a water inlet hole 10, an air pressure balance hole 11, a water outlet hole 12, a top cover 13, an air cooler 14, a fan 15, a temperature controller 16, a water pump power supply controller 17, a high-sensitivity temperature probe 18, a mobile power supply 19, a gas production pipe 20, a gas production valve 21, a waterproof cover 22, a sampling hole 23, a temperature probe jack 24 and a fan power supply hole 25. The method is characterized in that: the soil ring 1 is buried in a sample plot to be tested, the water tank 2 is fixedly bonded on the soil ring 1, and the refrigeration water tank 4 is connected with the box body 3; the box body 3 is placed in a water tank 2 filled with tap water; the refrigeration water tank 4 is fixed on the side surface of the box body 3 through a guide rail; the water pump 5 is placed at the bottom of the refrigeration water tank 4, and the cooling liquid 6 is directly poured into the refrigeration water tank 4; the water inlet pipe 7 is sleeved with the water outlet of the water pump 5, and the other end of the water inlet pipe 7 is sleeved with the water inlet at the bottom of the air cooler 13 through the water inlet hole 10; the air pressure balance pipe 8 is communicated with the air inside the box body 3 through an air pressure balance hole 11; one end of a water outlet pipe 9 is placed on the upper edge of the refrigerating water tank 4, and the other end of the water outlet pipe 9 is sleeved with a water outlet at the upper part of an air cooler 14 through a water outlet hole 12; the top cover 13 is hermetically bonded with the top of the box body 3; the top of the air cooler 14 is fixed with the inner side of the top cover 13; the fan 15 is fixed to the bottom of the air cooler 14; the temperature controller 16 is fixed on the outer side surface of the top cover 13, and the output end of the temperature controller 16 is connected with the water pump 5 through a water pump power controller 17; the high-sensitivity temperature probe 18 is connected with the temperature controller 16 through a temperature probe jack 24; the mobile power supply 19 is directly connected with the input end of the temperature controller 16 and the fan 15 through a fan power supply hole 25; one end of the sampling pipe 20 is communicated with the interior of the box body 3 through a sampling hole 23; the gas sampling valve 21 is fixed at the upper end of the sampling pipe 20; the waterproof cover 22 is fixed on the surface of the top cover 13 through an aluminum alloy hinge and covers the temperature controller 16, the water pump power controller 17 and the mobile power supply 19.
The soil ring 1 and the water tank 2 form a base, and the base is formed by bonding a PVC pipe with the pipe diameter of 390 mm, 400 mm or 410mm and a PVC plate with the thickness of 15 mm, 16 mm or 17 mm; the wall thickness of the box body 3 is 4 or 5 or 6mm, the pipe diameter is 390 or 400 or 410mm, and the height is 590 or 600 or 610 mm; the top cover 13 is made of a plexiglass plate with the thickness of 4 or 5 or 6mm and the diameter of 400 or 410 or 420 mm; the top cover 13 and the box body 3 are firmly bonded through organic glass glue, the pipe diameter of the air cooler 14 is 7 or 8 or 9mm, the wall thickness is 0.4 or 0.5 or 0.6mm, a red copper pipe with the length of 1.8 or 2 or 2.2m is wound into a cylindrical copper disc with the diameter of 140 or 150 or 160mm to be manufactured, the pipe diameter of the refrigeration water tank 4 is 90 or 100 or 110mm, the height of the refrigeration water tank 4 is 140 or 150 or 160mm, and the surface of the refrigeration water tank 4 is wrapped with heat insulation tinfoil paper and heat insulation foam so as to achieve the purpose of heat insulation.
The gas production device is formed by connecting a transparent silicone tube with the inner diameter of 5 or 6 or 7mm and the outer diameter of 9 or 10 or 11mm with a medical three-way valve, and the gas production device extends into the sampling box through a small hole (the aperture is 10mm) of a top cover. The air pressure balance tube is formed by connecting a transparent capillary tube with the inner diameter of 1 or 2 or 3mm, the outer diameter of 3 or 4 or 5mm and the length of 90 or 100 or 110mm with a water tank through a tank wall small hole (aperture of 4mm), one end of the air pressure balance tube is arranged in the sampling tank, and the other end of the air pressure balance tube is naturally arranged below the liquid level of the refrigeration water tank. And after all the accessories are installed, coating glass cement on all the small holes for sealing.
Through the technical steps, the temperature-controllable transparent static box is assembled.
Example 2:
an in-situ sampling method for greenhouse gases comprises the following steps:
step one, embedding a manufactured base (a soil ring 1 and a water tank 2) in advance at a sample point to be detected, wherein the embedding depth is 20 cm;
step two, the box body 3 is placed into the water tank 2, and the water tank 2 is filled with tap water;
step three, pouring the frozen cooling liquid 6 into a refrigeration water tank 4;
step four, connecting the mobile power supply 19 with the temperature controller 16 and the fan 15;
and step five, reading the instant temperature (air temperature-5 or-3 or-1 or 5 or 10 or 20 or 30 or 40 ℃) of the temperature controller 16, and setting the starting and stopping temperatures of the temperature controller 16 according to requirements. If the starting temperature is set as air temperature (-5 or-2 or 8 or 16 or 24 or 32 or 40 ℃) +0.1 ℃ (namely when the temperature in the box is higher than the air temperature by 0.1 ℃, the power supply of the water pump is switched on), the stopping temperature is set as air temperature (-5 or-1 or 7 or 15 or 21 or 28 or 32 or 36 or 40 ℃) to 0.1 ℃ (namely when the temperature in the box is lower than the air temperature by 0.1 ℃, the power supply of the water pump is switched off), and the temperature control precision at the moment is +/-0.1 ℃;
step six, turning on a power controller 17 of the water pump 5; at the moment, when the temperature in the box is higher than the air temperature (-5-40 ℃, any temperature in the interval) by 0.1 ℃, the refrigerating water pump works to circulate the cooling liquid into the air cooling device (red copper tube) so as to achieve the aim of quickly cooling; when the temperature in the refrigerator is lower than the air temperature (-5-40 ℃, any temperature in the interval) by 0.1 ℃, the refrigeration water pump stops working, and the low-temperature-18 or-14 or-10 or-6 or-3 or 0 ℃ cooling liquid in the cooling device flows back to the refrigeration water tank through the negative pressure of the water pump, thereby achieving the purpose of stopping cooling.
And step seven, opening the gas production valve 21 at the top end of the gas production pipe 20, rapidly collecting a 30ml gas sample by using a medical injector, then closing the gas production valve 21, and then collecting the gas sample once every 5 minutes.
And step eight, after 5 times of gas collection, turning off the water pump power supply controller 17, turning off the mobile power supply 19, taking down the box body 3, and recycling the cooling liquid 6.
And ninthly, analyzing the greenhouse gas concentration of the collected sample by a gas chromatograph (Agilent 7890A) as soon as possible, wherein the variation of the greenhouse gas concentration with time is the net exchange amount (NEE) of the greenhouse gas between the ecological system and the atmosphere.
Through the implementation steps, the technical problem that the temperature in the box rises too fast in field in-situ sampling of greenhouse gases is solved, the temperature in the sampling box is controllable, the influence of the temperature rise on the air pressure in the box and the photosynthesis of plants is avoided, and the accuracy of the NEE measuring result of the ecological system is ensured.
Claims (7)
1. The utility model provides a temperature controllable formula transparent static case, it is by soil ring (1), basin (2), box (3), refrigeration water tank (4), water pump (5), coolant liquid (6), inlet tube (7), atmospheric pressure balance pipe (8), outlet pipe (9), inlet opening (10), atmospheric pressure balance hole (11), apopore (12), top cap (13), air cooler (14), fan (15), temperature controller (16), water pump power supply controller (17), high sensitive temperature probe (18), portable power source (19), gas production pipe (20), gas production valve (21), buckler (22), sampling hole (23), temperature probe jack (24), fan power supply hole (25) are constituteed, its characterized in that: the base is composed of a soil ring (1) and a water tank (2), the soil ring (1) is buried in a sample plot to be tested, the water tank (2) is adhered and fixed on the soil ring (1), the refrigerating water tank (4) is connected with the box body (3), the box body (3) is placed in the water tank (2) filled with tap water, the refrigeration water tank (4) is fixed on the side surface of the box body (3) through a guide rail, the water pump (5) is placed at the bottom of the refrigeration water tank (4), the cooling liquid (6) is directly poured into the refrigeration water tank (4), the water inlet pipe (7) is sleeved with a water outlet of the water pump (5), the other end of the water inlet pipe (7) is sleeved with a water inlet at the bottom of the air cooler (14) through a water inlet hole (10), the air pressure balance pipe (8) is communicated with air in the box body (3) through an air pressure balance hole (11), one end of the water outlet pipe (9) is placed at the upper edge of the refrigeration water tank (4), and the other end of the water outlet pipe (9) is sleeved with a water outlet at the upper part of the air cooler (14) through a water outlet hole (12; the top cover (13) is hermetically bonded with the top of the box body (3); the top of the air cooler (14) is fixed with the inner side of the top cover (13); the fan (15) is fixed at the bottom of the air cooler (14), the temperature controller (16) is fixed at the outer side of the top cover (13), the output end of the temperature controller (16) is connected with the water pump (5) through a water pump power supply controller (17), the high-sensitivity temperature probe (18) is connected with the temperature controller (16) through a temperature probe jack (24), the mobile power supply (19) is directly connected with the input end of the temperature controller (16) and the fan (15) through a fan power supply hole (25), one end of the sampling pipe (20) is communicated with the interior of the box body (3) through a sampling hole (23), the sampling valve (21) is fixed at the upper end of the sampling pipe (20), and the waterproof cover (22) is fixed on the surface of the top cover (13) through an aluminum alloy hinge; the air pressure balance tube (8) is connected with the water tank (4) through a small hole on the tank wall by a transparent capillary tube with the inner diameter of 1-3 mm, the outer diameter of 3-5 mm and the length of 90-110 mm, one end of the air pressure balance tube (8) is arranged in the sampling tank, and the other end is naturally arranged below the liquid level of the refrigeration water tank.
2. A temperature-controllable transparent static chamber as claimed in claim 1, wherein: the soil ring (1) and the water tank (2) form a base, and the base is formed by bonding a PVC pipe with the pipe diameter of 390 and 410mm and a PVC plate with the thickness of 15-17 mm.
3. A temperature-controllable transparent static chamber as claimed in claim 1, wherein: the wall thickness of the box body (3) is 4-6 mm, the pipe diameter is 390-410 mm, and the height is 590-610 mm.
4. A temperature-controllable transparent static chamber as claimed in claim 1, wherein: the top cover (13) is made of an organic glass plate with the thickness of 4-6 mm and the diameter of 400-420 mm; the top cover (13) and the box body (3) are bonded through organic glass glue.
5. A temperature-controllable transparent static chamber as claimed in claim 1, wherein: the pipe diameter of the air cooler (14) is 7-9 mm, the wall thickness is 0.4-0.6 mm, and the copper tube with the length of 1.8-2.2 m is wound into a cylindrical copper disc with the diameter of 140-160 mm.
6. A temperature-controllable transparent static chamber as claimed in claim 1, wherein: the pipe diameter of the refrigeration water tank (4) is 90-110 mm, the height is 140-160 mm, and the surface of the refrigeration water tank (4) is wrapped by heat insulation tinfoil paper and heat insulation foam.
7. An in-situ greenhouse gas sampling method using the temperature-controllable transparent static chamber of claim 1, comprising the steps of:
A. burying a sampling box base into a sampling area one day in advance, and keeping a base water tank horizontal;
B. during sampling, the sampling box is placed in a base water tank, and a tap water sealing box body and a base are poured into the water tank;
C. injecting cooling liquid frozen at-18 ℃ for 11-13 hours into a refrigeration water tank;
D. connecting a temperature controller with a 12V mobile power supply, and measuring the instant temperature in the box and the air temperature to be-5-40 ℃;
E. setting the starting temperature of the temperature controller to be 0.1 ℃ higher than the air temperature and setting the stopping temperature to be 0.1 ℃ lower than the air temperature;
F. turning on a power switch of the water pump, and when the temperature in the box is 0.1 ℃ higher than the air temperature, circulating the cooling liquid into the air cooling device by the working of the refrigerating water pump to achieve cooling; when the temperature in the refrigerator is lower than the air temperature by 0.1 ℃, the refrigeration water pump stops working, and the cooling liquid with the temperature of minus 18 ℃ to 0 ℃ in the cooling device flows back to the refrigeration water tank through the negative pressure of the water pump to stop cooling;
G. collecting samples once every 5-10 minutes by a gas sampling device, collecting 30ml samples every time, and collecting 4-6 times in total;
H. after 4-6 times of gas collection, closing a water pump switch, disconnecting a power supply, taking down the static tank, and recovering the cooling liquid;
I. and (3) analyzing the greenhouse gas concentration of the collected sample by a gas chromatograph, wherein the variation of the greenhouse gas concentration along with time is the net exchange amount of the greenhouse gas between the ecological system and the atmosphere.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810097558.4A CN108318607B (en) | 2018-01-31 | 2018-01-31 | Temperature-controllable transparent static box and greenhouse gas field in-situ acquisition method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810097558.4A CN108318607B (en) | 2018-01-31 | 2018-01-31 | Temperature-controllable transparent static box and greenhouse gas field in-situ acquisition method |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN108318607A CN108318607A (en) | 2018-07-24 |
| CN108318607B true CN108318607B (en) | 2021-03-16 |
Family
ID=62890365
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810097558.4A Active CN108318607B (en) | 2018-01-31 | 2018-01-31 | Temperature-controllable transparent static box and greenhouse gas field in-situ acquisition method |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN108318607B (en) |
Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108871883A (en) * | 2018-07-26 | 2018-11-23 | 中国林业科学研究院林业研究所 | A kind of static chamber flux observation device and gas production method |
| CN109490146B (en) * | 2018-12-11 | 2020-05-26 | 中国科学院大气物理研究所 | Calibration system and method for land-atmosphere interface gas exchange flux |
| CN110031270A (en) * | 2019-04-26 | 2019-07-19 | 南京林业大学 | A kind of Soil Trace Gases flux measuring device and method |
| CN111983153B (en) * | 2020-08-31 | 2021-08-31 | 中国科学院地理科学与资源研究所 | System and method for detecting greenhouse gas emission of sediment and water body |
| CN113790933A (en) * | 2021-08-16 | 2021-12-14 | 天津大学 | Temperature-controlled windproof and light-resistant static box and gas in-situ collection method |
| CN117647626B (en) * | 2024-01-25 | 2024-04-26 | 中国科学院南京土壤研究所 | Device and method for synchronously monitoring greenhouse gas flux in field in-situ multi-region mode |
Family Cites Families (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102519759B (en) * | 2011-11-28 | 2014-03-12 | 中国科学院亚热带农业生态研究所 | Device for sampling nitrous oxide in paddy field soil |
| CN205157265U (en) * | 2015-11-05 | 2016-04-13 | 青岛明华电子仪器有限公司 | Atmospheric sampling ware constant temperature equipment |
| CN205679443U (en) * | 2016-06-20 | 2016-11-09 | 中国农业科学院草原研究所 | A kind of grassland soil greenhouse gases original-position collecting device |
| CN205861412U (en) * | 2016-08-05 | 2017-01-04 | 福建师范大学 | A kind of static chamber measuring Rhizophora apiculata Blume trunk methane transfer rate |
| CN206710422U (en) * | 2016-11-01 | 2017-12-05 | 青岛大学 | A kind of system of continuous on-line determination soil CH_4 uptake flux |
| CN206638464U (en) * | 2017-03-13 | 2017-11-14 | 环境保护部南京环境科学研究所 | A kind of field vegetation volatilization gas automatic acquisition device |
| CN106840783A (en) * | 2017-03-31 | 2017-06-13 | 华测检测认证集团股份有限公司 | Atmospheric sampling equipment with self-protection function |
| CN107121309A (en) * | 2017-05-16 | 2017-09-01 | 上海创蓝检测技术有限公司 | Condensable particulate matter CPM sample devices |
| CN107421787B (en) * | 2017-08-31 | 2023-08-15 | 北京市环境保护监测中心 | Sampling device and measuring method for total particulate matters in exhaust gas |
-
2018
- 2018-01-31 CN CN201810097558.4A patent/CN108318607B/en active Active
Also Published As
| Publication number | Publication date |
|---|---|
| CN108318607A (en) | 2018-07-24 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN108318607B (en) | Temperature-controllable transparent static box and greenhouse gas field in-situ acquisition method | |
| CN104267172B (en) | A kind of multifunctional all formula soil body freezing-thawing test system | |
| CN202486125U (en) | Experimental device for indoor frozen soil freezing and thawing circulation process | |
| CN202057528U (en) | Air flux static chamber in water-air interface | |
| CN204154664U (en) | Soil sample freezing-thawing test device | |
| CN104730100B (en) | A kind of device for testing aqueous porous media in frozen-thaw process reclaimed water thermal change feature | |
| CN206618597U (en) | A kind of float type water qi leel layer synchronized sampling unit | |
| CN102854384B (en) | Device for measuring temperature and resistivity distribution in phase-changing process in frozen soil test | |
| CN102590468A (en) | Testing system for deep soil freezing/thawing process | |
| CN107345894B (en) | A kind of the high pressure cold bench device and application method of in-situ observation gas hydrate size distribution | |
| CN102053145A (en) | In-situ automatic measurement method of CO2 of root system | |
| CN203465121U (en) | Static box for collecting greenhouse gas flux of greenhouse vegetable field | |
| CN203164111U (en) | Holder and holder-based acidizing etching instrument | |
| CN207423761U (en) | The indoor soil-column experimental provision infiltrated for Dripping simulation | |
| CN104062156A (en) | Greenhouse gas collection system and greenhouse gas emission analysis method | |
| CN102519759B (en) | Device for sampling nitrous oxide in paddy field soil | |
| CN103234971A (en) | Acidic etching instrument and operation method thereof | |
| CN104020097A (en) | Indoor measurement experiment instrument of hydraulic conductivity of unsaturated soil | |
| CN103901177A (en) | Measuring box and method for measuring exhausting flux of greenhouse gas on water surface | |
| CN208736779U (en) | A kind of tri-coupling type saturation cohesive soil constant head seepage flow test device | |
| CN201673107U (en) | Device for measuring saturated water conductivity of soil by variable hydraulic head method | |
| CN103207136A (en) | Device and method for measuring hydraulic permeability-electroosmosis coefficients | |
| CN103743883B (en) | A kind of apparatus and method of testing unsaturated soil hysteresis loop | |
| CN110895275A (en) | Device and method for measuring soil respiration and water migration by simulating temperature rise rainfall | |
| CN207318164U (en) | A kind of continuous carbon nitrogen isotope double labelling device of more time point samplings |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |