CN113755540A - Alanine production method based on membrane separation process - Google Patents
Alanine production method based on membrane separation process Download PDFInfo
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- CN113755540A CN113755540A CN202110903728.5A CN202110903728A CN113755540A CN 113755540 A CN113755540 A CN 113755540A CN 202110903728 A CN202110903728 A CN 202110903728A CN 113755540 A CN113755540 A CN 113755540A
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- membrane
- fermentation
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- nanofiltration membrane
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P13/00—Preparation of nitrogen-containing organic compounds
- C12P13/04—Alpha- or beta- amino acids
- C12P13/06—Alanine; Leucine; Isoleucine; Serine; Homoserine
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
- C07C227/00—Preparation of compounds containing amino and carboxyl groups bound to the same carbon skeleton
- C07C227/38—Separation; Purification; Stabilisation; Use of additives
- C07C227/40—Separation; Purification
Abstract
The invention belongs to the technical field of membrane separation, and particularly discloses an alanine production method based on a membrane separation process, wherein an ultrafiltration membrane is used for filtering, the filtered clear liquid is clear and bright, and all thalli, insoluble impurities and part of macromolecular substances can be removed; the nanofiltration membrane can separate small molecular substances and 40-60% of ash content, so that the product purity is improved, and the prepared alanine crude product has high purity and high yield; the energy consumption of ultrafiltration membrane and nanofiltration membrane concentration is about 1/5-1/4 of the energy consumption of the traditional heating concentration, and after the feed liquid is reduced through nanofiltration concentration, the energy consumption of subsequent drying is greatly reduced; after being concentrated by the ultrafiltration membrane and the nanofiltration membrane, the nano-membrane has no phase change, no additive, no chemical change and safe product quality; the whole operation flow is simple, the equipment design is compact, the operation is easy for workers, and the labor intensity is low; the equipment occupies small area, the investment is small, the operation cost is low, and the investment recovery period is short; the full-closed pipeline type operation is adopted, the external environment is clean, and the requirements of FDA production specifications are completely met.
Description
Technical Field
The invention relates to the technical field of membrane separation, in particular to an alanine production method based on a membrane separation process.
Background
The existing amino acid production process comprises an enzymatic conversion method and a fermentation method, wherein the cost for producing the amino acid by the enzymatic conversion method is higher, and the cost for producing the amino acid by the fermentation method is lower. With the continuous development and maturity of fermentation technology, amino acid production is more and more favored and advocated by manufacturers.
At present, the alanine is produced by adopting an enzyme conversion method basically, when the alanine is produced by a fermentation method, the fermentation liquor contains a large amount of thalli, proteins, pigments, various ions, salts and the like, the quality of the produced alanine finished product is severely restricted, and if the original plate-and-frame filtration and activated carbon adsorption decoloration methods are adopted, the cost is higher, the product yield is low, and the treatment adsorption effect is not ideal.
Disclosure of Invention
The present invention aims at providing one kind of alanine producing process based on membrane separation process to solve the problems of the background technology.
In order to achieve the purpose, the invention provides the following technical scheme:
a method for producing alanine based on membrane separation process comprises the following steps:
s1, inoculating the escherichia coli seed liquid into a fermentation tank filled with a fermentation substrate, and carrying out fermentation culture for 50-70h at 37 ℃ to obtain a fermentation liquid;
s2, filtering the fermentation liquor by using a disc centrifuge to remove large-particle impurities, washing the centrifuged residue for 2-3 times by using water, and mixing the washing liquid and the centrifugate to obtain a raw material liquid;
s3, filtering the raw material liquid in the previous step by using an ultrafiltration membrane to remove impurities, removing all thalli, insoluble substances and part of macromolecular substances in the material liquid, adding pure water for dialysis into the residual material liquid before the filtration is finished, and then filtering the material liquid added with the pure water by using the ultrafiltration membrane;
s4, passing the raw material liquid through an ultrafiltration membrane to filter the clear liquid, concentrating the clear liquid by using a nanofiltration membrane, and sequentially passing through three stages of nanofiltration membranes to concentrate respectively, wherein the solid content of the concentrated liquid is 20-30% and the ash removal rate is 40-60%;
s5, putting the prepared concentrated solution into a vessel, and carrying out evaporation concentration;
s6, drying the concentrated object prepared by the above method by a proper drying method to obtain the crude product of alanine.
Preferably, the fermentation substrate in step S1 is a fermentation medium, and the fermentation medium is prepared from the following raw materials: glucose, potassium dihydrogen phosphate, citric acid, a trace element concentrated solution, vitamin B1, diammonium hydrogen phosphate and magnesium sulfate.
Preferably, in the fermentation process, the fermentation medium is stirred by a stirring motor in a fermentation tank, the pH value of the fermentation medium is 5.5-6.5, and the air flow in the fermentation tank is 2-3L/min.
Preferably, when the fermentation broth is filtered by the disk centrifuge in step S2, the temperature of the fermentation broth is reduced to 20-25 ℃, and the centrifugation speed of the disk centrifuge is 4000-.
Preferably, the ultrafiltration membrane of step S3 is one of a ceramic ultrafiltration membrane, a tubular ultrafiltration membrane and a roll-type ultrafiltration membrane.
Preferably, in step S3, the dialysis pure water is added in an amount of 10-30 wt% based on the raw material solution, and the ultrafiltration membrane is operated at a pressure of 0.1-0.5 MPa.
Preferably, the nanofiltration membrane in the step S4 is an organic roll-type nanofiltration membrane, the three-stage nanofiltration membrane is concentrated by three nanofiltration membranes, the cutting molecular weight of the first-stage nanofiltration membrane is 250-350Da, the cutting molecular weight of the second-stage nanofiltration membrane is 200-300Da, the cutting molecular weight of the third-stage nanofiltration membrane is 150-250Da, and the operating pressure of the three-stage nanofiltration membrane is 1-3 MPa.
Preferably, the effective membrane area of the three-stage nanofiltration membrane is 25-35m2And the temperature of the three-stage nanofiltration membrane is 10-60 ℃ when the three-stage nanofiltration membrane is sequentially concentrated.
Compared with the prior art, the invention has the beneficial effects that:
1. filtering with ultrafiltration membrane, clarifying the filtered clear liquid, and removing all thallus, insoluble impurities and part of macromolecular substances; the nanofiltration membrane can separate small molecular substances and 40-60% of ash content, so that the product purity is improved;
2. the energy consumption of concentration by the ultrafiltration membrane and the nanofiltration membrane is about 1/5-1/4 of the energy consumption of the traditional heating concentration, and after the feed liquid is reduced by nanofiltration concentration, the energy consumption of subsequent drying is greatly reduced;
3. after being concentrated by the ultrafiltration membrane and the nanofiltration membrane, the nano-membrane has no phase change, no additive, no chemical change and safe product quality;
4. the whole operation flow is simple, the equipment design is compact, the operation is easy for workers, and the labor intensity is low;
5. the equipment occupies small area, the investment is small, the operation cost is low, and the investment recovery period is short;
6. the full-closed pipeline type operation is adopted, the external environment is clean, and the requirements of FDA production specifications are completely met.
Detailed Description
The technical solutions in the embodiments of the present invention are clearly and completely described below, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
The invention provides a technical scheme that: a method for producing alanine based on membrane separation process comprises the following steps:
s1, inoculating the escherichia coli seed liquid into a fermentation tank filled with a fermentation substrate, and carrying out fermentation culture for 50-70h at 37 ℃ to obtain a fermentation liquid;
s2, filtering the fermentation liquor by using a disc centrifuge to remove large-particle impurities, washing the centrifuged residue for 2-3 times by using water, and mixing the washing liquid and the centrifugate to obtain a raw material liquid;
s3, filtering the raw material liquid in the previous step by using an ultrafiltration membrane to remove impurities, removing all thalli, insoluble substances and part of macromolecular substances in the material liquid, adding pure water for dialysis into the residual material liquid before the filtration is finished, and then filtering the material liquid added with the pure water by using the ultrafiltration membrane;
s4, passing the raw material liquid through an ultrafiltration membrane to filter the clear liquid, concentrating the clear liquid by using a nanofiltration membrane, and sequentially passing through three stages of nanofiltration membranes to concentrate respectively, wherein the solid content of the concentrated liquid is 20-30% and the ash removal rate is 40-60%;
s5, putting the prepared concentrated solution into a vessel, and carrying out evaporation concentration;
s6, drying the concentrated object prepared by the above method by a proper drying method to obtain the crude product of alanine.
Further, in step S1, the fermentation substrate is a fermentation medium, and the fermentation medium comprises the following raw materials: glucose, potassium dihydrogen phosphate, citric acid, a trace element concentrated solution, vitamin B1, diammonium hydrogen phosphate and magnesium sulfate.
Further, in the fermentation process, stirring is carried out by a stirring motor in the fermentation tank, the pH value of the fermentation medium is 5.5-6.5, and the air flow in the fermentation tank is 2-3L/min.
Further, when the fermentation liquid is filtered by the disk centrifuge in step S2, the temperature of the fermentation liquid is reduced to 20-25 ℃, and the centrifugal speed of the disk centrifuge is 4000-.
Further, the ultrafiltration membrane of step S3 is one of a ceramic ultrafiltration membrane, a tubular ultrafiltration membrane and a roll-type ultrafiltration membrane.
Furthermore, in step S3, the mass of the dialysis pure water added accounts for 10-30% of the weight of the raw material liquid, and the running pressure of the ultrafiltration membrane is 0.1-0.5 MPa.
Further, the nanofiltration membrane in step S4 is an organic roll-type nanofiltration membrane, the three-stage nanofiltration membrane is concentrated by three nanofiltration membranes, the cutting molecular weight of the first-stage nanofiltration membrane is 250-350Da, the cutting molecular weight of the second-stage nanofiltration membrane is 200-300Da, the cutting molecular weight of the third-stage nanofiltration membrane is 150-250Da, and the operating pressure of the three-stage nanofiltration membrane is 1-3 MPa.
Furthermore, the effective membrane area of the three-stage nanofiltration membrane is 25-35m2And the temperature of the three-stage nanofiltration membrane is 10-60 ℃ when the three-stage nanofiltration membrane is sequentially concentrated.
Filtering with ultrafiltration membrane, clarifying the filtered clear liquid, and removing all thallus, insoluble impurities and part of macromolecular substances; the nanofiltration membrane can separate small molecular substances and 40-60% of ash content, so that the product purity is improved, and the prepared alanine crude product has high purity and high yield;
the energy consumption of concentration by the ultrafiltration membrane and the nanofiltration membrane is about 1/5-1/4 of the energy consumption of the traditional heating concentration, and after the feed liquid is reduced by nanofiltration concentration, the energy consumption of subsequent drying is greatly reduced;
after being concentrated by the ultrafiltration membrane and the nanofiltration membrane, the nano-membrane has no phase change, no additive, no chemical change and safe product quality;
the whole operation flow is simple, the equipment design is compact, the operation is easy for workers, and the labor intensity is low;
the equipment occupies small area, the investment is small, the operation cost is low, and the investment recovery period is short;
the full-closed pipeline type operation is adopted, the external environment is clean, and the requirements of FDA production specifications are completely met.
Compared with the traditional process, the process can realize long-term stable continuous industrial production.
Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.
Claims (8)
1. A method for producing alanine based on membrane separation technology is characterized in that: the method comprises the following steps:
s1, inoculating the escherichia coli seed liquid into a fermentation tank filled with a fermentation substrate, and carrying out fermentation culture for 50-70h at 37 ℃ to obtain a fermentation liquid;
s2, filtering the fermentation liquor by using a disc centrifuge to remove large-particle impurities, washing the centrifuged residue for 2-3 times by using water, and mixing the washing liquid and the centrifugate to obtain a raw material liquid;
s3, filtering the raw material liquid in the previous step by using an ultrafiltration membrane to remove impurities, removing all thalli, insoluble substances and part of macromolecular substances in the material liquid, adding pure water for dialysis into the residual material liquid before the filtration is finished, and then filtering the material liquid added with the pure water by using the ultrafiltration membrane;
s4, passing the raw material liquid through an ultrafiltration membrane to filter the clear liquid, concentrating the clear liquid by using a nanofiltration membrane, and sequentially passing through three stages of nanofiltration membranes to concentrate respectively, wherein the solid content of the concentrated liquid is 20-30% and the ash removal rate is 40-60%;
s5, putting the prepared concentrated solution into a vessel, and carrying out evaporation concentration;
s6, drying the concentrated object prepared by the above method by a proper drying method to obtain the crude product of alanine.
2. The method for producing alanine based on membrane separation process according to claim 1, characterized in that: in the step S1, the fermentation substrate is a fermentation medium, and the fermentation medium comprises the following raw materials: glucose, potassium dihydrogen phosphate, citric acid, a trace element concentrated solution, vitamin B1, diammonium hydrogen phosphate and magnesium sulfate.
3. The method for producing alanine based on membrane separation process according to claim 2, characterized in that: in the fermentation process, stirring is carried out by a stirring motor in a fermentation tank, the pH value of a fermentation medium is 5.5-6.5, and the air flow in the fermentation tank is 2-3L/min.
4. The method for producing alanine based on membrane separation process according to claim 1, characterized in that: when the fermentation liquid is filtered by the disk centrifuge in step S2, the temperature of the fermentation liquid is reduced to 20-25 ℃, and the centrifugal speed of the disk centrifuge is 4000-.
5. The method for producing alanine based on membrane separation process according to claim 1, characterized in that: the step S3 ultrafiltration membrane is one of a ceramic ultrafiltration membrane, a tubular ultrafiltration membrane and a roll-type ultrafiltration membrane.
6. The method for producing alanine based on membrane separation process according to claim 1, characterized in that: in step S3, the pure water for dialysis is added in an amount of 10-30 wt% based on the weight of the raw material solution, and the operating pressure of the ultrafiltration membrane is 0.1-0.5 MPa.
7. The method for producing alanine based on membrane separation process according to claim 1, characterized in that: the nanofiltration membrane is an organic rolling type nanofiltration membrane in the step S4, the three-stage nanofiltration membrane is concentrated through the three-stage nanofiltration membrane, the cutting molecular weight of the first-stage nanofiltration membrane is 250-350Da, the cutting molecular weight of the second-stage nanofiltration membrane is 200-300Da, the cutting molecular weight of the third-stage nanofiltration membrane is 150-250Da, and the operating pressure of the three-stage nanofiltration membrane is 1-3 MPa.
8. The method for producing alanine based on membrane separation process according to claim 1, characterized in that: the effective membrane area of the three-stage nanofiltration membrane is 25-35m2And the temperature of the three-stage nanofiltration membrane is 10-60 ℃ when the three-stage nanofiltration membrane is sequentially concentrated.
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| CN101812009A (en) * | 2010-04-28 | 2010-08-25 | 河南巨龙淀粉实业有限公司 | Novel technique for extracting L-tryptophan from fermentation broth |
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