CN113754792A - Preparation method of wolfberry extract containing wolfberry polysaccharide - Google Patents
Preparation method of wolfberry extract containing wolfberry polysaccharide Download PDFInfo
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- CN113754792A CN113754792A CN202111251679.8A CN202111251679A CN113754792A CN 113754792 A CN113754792 A CN 113754792A CN 202111251679 A CN202111251679 A CN 202111251679A CN 113754792 A CN113754792 A CN 113754792A
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- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
- C08B37/0003—General processes for their isolation or fractionation, e.g. purification or extraction from biomass
Abstract
The invention relates to the technical field of functional food preparation, in particular to a preparation method of a lycium barbarum extract containing lycium barbarum polysaccharide, which comprises the following process steps: (1) micronizing fructus Lycii to obtain fructus Lycii fine powder; (2) adding vitamin C into the fine wolfberry powder to obtain a mixture; (3) adding enzyme into the mixture for ultrasonic extraction, wherein the enzyme is mixed enzyme consisting of cellulase, pectinase and papain; (4) centrifuging the enzymolysis extracting solution, and collecting supernatant; (5) and (3) carrying out ultrafiltration separation on the supernatant by using an ultrafiltration membrane system to obtain a product with a molecular weight section of 10-50kDa, and then carrying out freeze drying on the product to obtain the wolfberry extract containing wolfberry polysaccharide. The invention has the beneficial effects that: the invention combines physical degradation and biological degradation without adding any additive with side effect. The extraction process of the lycium barbarum polysaccharide is green, environment-friendly and safe, and the prepared lycium barbarum polysaccharide is high in extraction rate and purity and strong in antioxidant activity.
Description
Technical Field
The invention relates to the technical field of functional food preparation, in particular to a preparation method of a lycium barbarum extract containing lycium barbarum polysaccharide.
Background
Lycium chinense (Lycium chinense Milll) belongs to Solanaceae, is a traditional Chinese medicinal material used as both medicine and food, has sweet taste and mild nature, and has the functions of nourishing liver and kidney, benefiting essence and blood and improving eyesight. The lycium barbarum polysaccharide is a main effective component of lycium barbarum, is a compound polysaccharide consisting of acidic heteropolysaccharide and polypeptide or protein, has a large molecular weight and a very complex structure composition, has the effects of resisting bacteria, tumors and aging and protecting liver, and is also an active substance of the lycium barbarum for reducing blood sugar, blood pressure, fat and inflammation and the like.
Chinese patent CN101029088 discloses a preparation method of lycium barbarum polysaccharide, wherein lycium barbarum polysaccharide is separated and purified by using column chromatography by using fresh lycium barbarum fruits as raw materials. The whole process comprises the working procedures of soaking, crushing, extracting, enzymolysis, column chromatography, alcohol precipitation, dealcoholization, drying and the like, the extraction solution is treated by pectinase, the purity of the polysaccharide is improved, but the selected enzyme is single, the crushing is conventional crushing, the yield and the antioxidant activity of the polysaccharide cannot be further improved, the raw materials are not fully utilized, and additives such as ethanol and the like which are not beneficial to health are used.
At present, conventional extraction methods of plant polysaccharides comprise a water extraction and alcohol precipitation method, a microwave extraction method, an acid-base decomposition extraction method and the like, most of the conventional methods need high-temperature treatment and the like to be unfavorable for protecting the activity of the polysaccharides, and chemical degradation methods such as water extraction and alcohol precipitation and the like are not environment-friendly enough and can bring health problems. The conventional method for preparing the lycium barbarum polysaccharide also easily causes the defects of easy chain scission of molecular weight, deepened browning of product color, loss of biological activity and the like.
Disclosure of Invention
The technical problem to be solved by the invention is as follows: provides a preparation method of the lycium barbarum extract containing lycium barbarum polysaccharide, which is green and environment-friendly and has high extraction rate and purity.
The technical scheme provided by the invention is as follows: provides a preparation method of a medlar extract containing medlar polysaccharide, which comprises the following process steps:
(1) micronizing fructus Lycii to obtain fructus Lycii fine powder;
(2) adding 8-12 times of water by weight into the medlar fine powder, and adding vitamin C to obtain a mixture;
(3) adding enzyme into the mixture for ultrasonic extraction to obtain an enzymolysis extracting solution; the enzyme is a mixed enzyme consisting of cellulase, pectinase and papain;
(4) centrifuging the enzymolysis extracting solution, and collecting supernatant;
(5) and (3) carrying out ultrafiltration separation on the supernatant by using an ultrafiltration membrane system to obtain a product with a molecular weight of 10-50kDa, and freeze-drying the product to obtain freeze-dried powder, namely the lycium barbarum extract containing lycium barbarum polysaccharide.
The invention has the beneficial effects that: the preparation method of the lycium barbarum extract containing lycium barbarum polysaccharide combines physical degradation and biodegradation, no additive with side effect is added, the extraction process is green, environment-friendly and safe, and the prepared lycium barbarum polysaccharide is high in extraction rate and purity and strong in antioxidant activity. The extraction rate of the existing ultrafiltration polysaccharide extraction method is low, in the preparation method, the medlar is firstly subjected to superfine grinding technology to destroy the cell structure, then is soaked by vitamin C, and finally is subjected to ultrasonic enzyme combined extraction, so that the extraction rate of the medlar polysaccharide is improved to the greatest extent, and the vitamin C is used for protecting the extract, so that the obtained extract has the advantage of white color.
Detailed Description
In order to explain the technical content, the objects and the effects of the present invention in detail, the following description will be given with reference to the embodiments.
The invention provides a preparation method of a wolfberry extract containing wolfberry polysaccharide, which comprises the following process steps:
(1) micronizing fructus Lycii to obtain fructus Lycii fine powder;
(2) adding 8-12 times of water by weight into the medlar fine powder, and adding vitamin C to obtain a mixture;
(3) adding enzyme into the mixture for ultrasonic extraction to obtain an enzymolysis extracting solution; the enzyme is a mixed enzyme consisting of cellulase, pectinase and papain;
(4) centrifuging the enzymolysis extracting solution, and collecting supernatant;
(5) and (3) carrying out ultrafiltration separation on the supernatant by using an ultrafiltration membrane system to obtain a product with a molecular weight of 10-50kDa, and freeze-drying the product to obtain freeze-dried powder, namely the lycium barbarum extract containing lycium barbarum polysaccharide.
The invention combines physical degradation and biological degradation without adding any additive with side effect, the polysaccharide extraction process is green, environment-friendly and safe, and the prepared lycium barbarum polysaccharide has high extraction rate and purity and strong antioxidant activity.
The invention adopts the superfine grinding technology to pretreat the material, and the superfine grinding technology can greatly destroy the cell structure and even break the cell wall, so that the polysaccharide component existing in the cell is exposed, and the polysaccharide extraction rate can be improved. Meanwhile, the granularity of the extract is greatly reduced, the contact area of the extract and an extraction solvent is increased, and the dissolution speed of the polysaccharide is improved; the invention also protects the polysaccharide active polysaccharide molecules from being easily broken by vitamin C, so that the finally prepared wolfberry polysaccharide product has good color and luster, and can further protect the antioxidant activity of the wolfberry polysaccharide from being damaged; the invention also assists ultrasonic enzyme extraction treatment, and a plurality of enzymes are combined for use to more fully hydrolyze pectin, cellulose, polysaccharide and the like in the medlar, decompose cell wall components, destroy cell wall structures, generate local collapse, dissolution and loosening, reduce resistance from cell walls and intercellular substances during extraction, accelerate the speed of dissolving out cells of effective components and improve extraction efficiency. And enzymolysis is carried out on protein and other impurities, so that the polysaccharide is extracted more thoroughly and has higher purity. The lycium barbarum polysaccharides with the molecular weight of 10-50kDa extracted by ultrafiltration comprise polysaccharides of the effective part of lycium barbarum, and the balance of monosaccharides with small molecular weight or impurities in larger molecules.
Preferably, in the preparation method of the lycium barbarum extract containing lycium barbarum polysaccharide, the step (1) is specifically as follows: pulverizing fructus Lycii, drying at 40-50 deg.C, and micronizing the coarse powder in micronizer to obtain 300-400 mesh fructus Lycii fine powder.
Preferably, in the above method for preparing an extract of lycium barbarum containing lycium barbarum polysaccharides, the step (2) further comprises: the mixture is rapidly cooled to-20 to-16 ℃, frozen for 2 to 4 hours and then rapidly thawed by microwave, and the microwave power is 3500 and 5000W. Repeating the steps of rapidly cooling to-20 to-16 ℃, freezing for 2-4 hours and then rapidly thawing by microwave for 3 times. The rapid cooling to-20 to-16 ℃ means that the temperature is reduced to-20 to-16 ℃ within 1-3 min.
As can be seen from the above description, in the low-temperature freeze-thaw process of the present invention, a huge temperature difference is generated due to uneven temperature changes inside and outside the cell, and the thermal shock effect caused by the temperature difference can effectively destroy the cell structure, so that the polysaccharide components inside the cell can be sufficiently dissolved out, the polysaccharide leaching rate can be effectively improved, and the low-temperature freeze-thaw extraction process is always performed at a lower temperature, thereby effectively reducing the loss of the active components of the polysaccharide.
Preferably, in the above method for preparing an extract of lycium barbarum containing lycium barbarum polysaccharides, the step (2) is specifically: adding 8-12 times of water into the fructus Lycii fine powder, adding vitamin C and edible acid, and treating for 30-60min to obtain mixture.
From the above description, the present invention further decomposes cell walls by adding edible acid, thereby improving extraction yield.
Preferably, in the preparation method of the lycium barbarum extract containing lycium barbarum polysaccharide, the addition amount of the vitamin C is 1.5-3g/L, the mass concentration of the edible acid in the mixture after the edible acid is added is 3-5%, and the edible acid is acetic acid.
Preferably, in the preparation method of the lycium barbarum extract containing lycium barbarum polysaccharide, the addition amount of the enzyme is 1-3% of the mixture by mass, and the addition mass ratio of the cellulase, the pectinase and the papain is 1:2: 3.
Preferably, in the preparation method of the lycium barbarum extract containing lycium barbarum polysaccharide, the ultrasonic extraction conditions are as follows: the temperature is 20-40 ℃; the ultrasonic power is 500-1700W; the circulating stirring speed is 500-2000 rpm; duty ratio 1: 1-1: 3; ultrasonic extraction time is 0.5-2 h.
Preferably, in the above method for preparing an extract of lycium barbarum containing lycium barbarum polysaccharides, the step (4) is specifically: centrifuging the enzymolysis extracting solution at 4000-.
The preparation method of the lycium barbarum extract containing lycium barbarum polysaccharide has the following advantages:
1. the invention adopts the superfine powder technology, improves the extraction efficiency, reduces the molecular weight of the extracted polysaccharide and ensures that the prepared polysaccharide has higher antioxidant activity.
2. According to the invention, the active polysaccharide molecules of the polysaccharide are protected by the vitamin C, so that the chains are not easily broken, the finally prepared wolfberry polysaccharide product has good color and luster, and the antioxidant activity of the wolfberry polysaccharide is further improved. The invention can be added with edible acid to further decompose cell walls and synergistically improve the vitamin C protection function.
3. The invention also assists ultrasonic enzyme extraction treatment, and a plurality of enzymes are combined for use to more fully hydrolyze pectin, cellulose, polysaccharide and the like in the medlar, decompose cell wall components, destroy cell wall structures, generate local collapse, dissolution and loosening, reduce resistance from cell walls and intercellular substances during extraction, accelerate the speed of dissolving out cells of effective components and improve extraction efficiency. And enzymolysis is carried out on protein and other impurities, so that the polysaccharide is extracted more thoroughly and has higher purity.
4. The lycium barbarum polysaccharide with the molecular weight of 10-50kDa extracted by ultrafiltration comprises the polysaccharide of the effective part of lycium barbarum, and the impurities in the rest monosaccharide with small molecular weight or larger molecules are discarded, so that the purity of the polysaccharide is improved.
5. The invention also adopts a low-temperature freeze thawing technology, and utilizes the thermal shock effect generated in the freezing-thawing process to destroy the cell structure, thereby achieving the purpose of increasing the extraction rate and activity of the polysaccharide.
Example 1
A preparation method of a medlar extract containing medlar polysaccharide comprises the following process steps:
(1) pulverizing fructus Lycii, oven drying at 30 deg.C, and micronizing the coarse powder in micronizer to obtain 400-mesh fructus Lycii fine powder.
(2) Adding 10 times of water by weight of the medlar fine powder, and adding vitamin C to obtain a mixture; the addition amount of the vitamin C is 2 g/L.
(3) Adding enzyme into the mixture obtained in the step (2) for ultrasonic extraction to obtain an enzymolysis extracting solution; the ultrasonic extraction conditions are as follows: the temperature is 20 ℃; the ultrasonic power is 500W; the circulating stirring speed is 500 rpm; duty ratio 1: 3; ultrasonic extraction time is 0.5 h; the adding mass percentage of the enzyme in the mixture is 1%, and the adding mass ratio of the cellulase, the pectinase and the papain is 1:2: 3.
(4) Centrifuging the enzymolysis extracting solution at the rotating speed of 4000r/min for 10min, and collecting the supernatant.
(5) And (3) carrying out ultrafiltration separation on the supernatant by using an ultrafiltration membrane system to obtain a product with a molecular weight of 10-50kDa, and freeze-drying the product to obtain freeze-dried powder, namely the lycium barbarum extract containing lycium barbarum polysaccharide.
Example 2
A preparation method of a medlar extract containing medlar polysaccharide comprises the following process steps:
(1) pulverizing fructus Lycii, oven drying at 30 deg.C, and micronizing the coarse powder in micronizer to obtain 400-mesh fructus Lycii fine powder.
(2) Adding 10 times of water by weight into the medlar fine powder, and then adding vitamin C and acetic acid for treatment for 50min to obtain a mixture; the addition amount of the vitamin C is 2g/L, and the mass concentration of the acetic acid in the mixture after the acetic acid is added is 2%.
And (3) reducing the temperature of the mixture to-20 ℃ within 2min, freezing for 2 hours, and then rapidly thawing by microwave, wherein the microwave power is 3500W. Repeating the steps of rapidly cooling to-20 ℃, freezing for 2 hours and then rapidly thawing by microwave for 3 times.
(3) Adding enzyme into the mixture obtained in the step (2) for ultrasonic extraction to obtain an enzymolysis extracting solution; the ultrasonic extraction conditions are as follows: the temperature is 20 ℃; the ultrasonic power is 500W; the circulating stirring speed is 500 rpm; duty ratio 1: 3; ultrasonic extraction time is 0.5 h;
the adding mass percentage of the enzyme in the mixture is 1%, and the adding mass ratio of the cellulase, the pectinase and the papain is 1:2: 3.
(4) Centrifuging the enzymolysis extracting solution at the rotating speed of 4000r/min for 10min, and collecting the supernatant.
(5) And (3) carrying out ultrafiltration separation on the supernatant by using an ultrafiltration membrane system to obtain a product with a molecular weight of 10-50kDa, and freeze-drying the product to obtain freeze-dried powder, namely the lycium barbarum extract containing lycium barbarum polysaccharide.
Example 3
The procedure of example 1 was repeated except that the ultrafine pulverization in step (1) was not conducted to obtain a fine powder of Lycium chinense having a mesh size of 50 mesh, as compared with example 2.
Example 4
Same as example 1 except that no vitamin C was added in step (1) compared to example 2.
Example 5
The procedure was as in example 1 except that the rapid cooling and microwave thawing in step (2) were not conducted, as compared with example 2.
Example 6
Compared with the example 2, the method is the same as the example 1 except that the ultrafiltration separation in the step (5) obtains the product with the molecular weight section of 1-100 kDa.
The purity, extraction rate and DPPH clearance of the Lycium barbarum polysaccharides of the Lycium barbarum extracts prepared in examples 1-6 are shown in Table 1.
TABLE 1
Compared with the example 1, the purity, the extraction rate and the antioxidant activity of the lycium barbarum polysaccharide are greatly improved by performing microwave thawing treatment after edible acid and rapid cooling;
as can be seen from the comparison between the example 3 and the example 2, the ultra-micro extraction has a great effect on the extraction rate and the antioxidant activity of the lycium barbarum polysaccharide;
as can be seen from the comparison between example 4 and example 2, vitamin C has a greater effect on protecting the activity of polysaccharides and improving the antioxidant activity of lycium barbarum polysaccharides;
as can be seen from the comparison between example 5 and example 2, the microwave thawing after the rapid cooling also has a great effect on the extraction rate of the lycium barbarum polysaccharide.
From the above results, it can be seen that: according to the invention, by adopting a combined extraction mode of ultramicro extraction, vitamin C and edible acid addition, cooling and microwave thawing, compared with the mode lacking one of the extraction modes, the prepared wolfberry extract containing wolfberry polysaccharide has higher purity, extraction rate and oxidation resistance. Thus, the synergistic effect is achieved between the combination of vitamin C and edible acid, cooling, microwave thawing and ultrafiltration separation extraction.
Example 7
A preparation method of a medlar extract containing medlar polysaccharide comprises the following process steps:
(1) pulverizing fructus Lycii, oven drying at 50 deg.C, and micronizing the coarse powder in micronizer to obtain 400 mesh fructus Lycii fine powder.
(2) Adding 12 times of water into the medlar fine powder, and then adding vitamin C and edible acid for treatment for 60min to obtain a mixture; the addition amount of the vitamin C is 3g/L, the mass concentration of the added edible acid in the mixture is 5%, and the edible acid is citric acid.
And (3) reducing the temperature of the mixture to-20 ℃ within 3min, freezing for 4 hours, and then quickly thawing by microwave, wherein the microwave power is 5000W.
Repeating the steps of rapidly cooling to-20 ℃, freezing for 4 hours and then rapidly thawing by microwave for 3 times.
(3) Adding enzyme into the mixture obtained in the step (2), adding a buffer solution to prepare a pH value of 5, wherein the buffer solution is a mixed buffer solution of citric acid and sodium citrate, and then carrying out ultrasonic extraction to obtain an enzymolysis extracting solution;
the ultrasonic extraction conditions are as follows: the temperature is 40 ℃; the ultrasonic power is 1700W; the circulating stirring speed is 2000 rpm; duty ratio 1: 1; ultrasonic extraction time is 2 h;
the adding mass percentage of the enzyme in the mixture is 3%, and the adding mass ratio of the cellulase to the pectinase to the papain is 1:2: 3.
(4) Centrifuging the enzymolysis extractive solution at 5000r/min for 15min, and collecting supernatant.
(5) And (3) carrying out ultrafiltration separation on the supernatant by using an ultrafiltration membrane system to obtain a product with a molecular weight of 10-50kDa, and freeze-drying the product to obtain freeze-dried powder, namely the lycium barbarum extract containing lycium barbarum polysaccharide. The pressure of the vacuum freeze drying treatment is 0.5-0.6 mbar, and the temperature is-5-30 ℃.
The extract obtained in this example had a purity of 75.2% for lycium barbarum polysaccharides and an extraction rate of 95.7%.
Example 8
A preparation method of a medlar extract containing medlar polysaccharide comprises the following process steps:
(1) pulverizing fructus Lycii, oven drying at 40 deg.C, and micronizing the coarse powder in micronizer to obtain 300-mesh fructus Lycii fine powder.
(2) Adding 10 times of water by weight into the medlar fine powder, and then adding vitamin C and edible acid for treatment for 45min to obtain a mixture; the addition amount of the vitamin C is 2g/L, the mass concentration of the edible acid in the mixture after the edible acid is added is 4%, and the edible acid is acetic acid.
And (3) cooling the mixture to-18 ℃ within 2min, freezing for 3 hours, and then quickly thawing by using microwaves, wherein the microwave power is 4000W. Repeating the steps of rapidly cooling to-18 ℃, freezing for 3 hours and then rapidly thawing by microwave for 3 times.
(3) Adding enzyme into the mixture obtained in the step (2), adding a buffer solution to prepare a pH value of 4, wherein the buffer solution is a mixed buffer solution of citric acid and sodium citrate, and then carrying out ultrasonic extraction to obtain an enzymolysis extracting solution;
the ultrasonic extraction conditions are as follows: the temperature is 30 ℃; the ultrasonic power is 1000W; the circulating stirring speed is 1000 rpm; duty ratio 1: 1-1: 3; ultrasonic extraction time is 0.5-2 h;
the adding mass percentage of the enzyme in the mixture is 2%, and the adding mass ratio of the cellulase to the pectinase to the papain is 1:2: 3.
(4) Centrifuging the enzymolysis extracting solution at the rotating speed of 4000-.
(5) And (3) carrying out ultrafiltration separation on the supernatant by using an ultrafiltration membrane system to obtain a product with a molecular weight range of 10-50kDa, and then carrying out freeze drying on the product. The obtained freeze-dried powder is the medlar extract containing medlar polysaccharide.
The ultrafiltration separation specifically comprises the following steps: performing ultrafiltration with ultrafiltration device with molecular weight cut-off of 10kDa to retain solution with molecular weight greater than 10kDa, and performing ultrafiltration with ultrafiltration device with molecular weight cut-off of 50kDa to retain solution with molecular weight less than 50 kDa.
The purity of the lycium barbarum polysaccharide of the lycium barbarum extract containing lycium barbarum polysaccharide obtained in the example is 77.6%, and the extraction rate is 96.3%.
The lycium barbarum polysaccharide of the lycium barbarum extract containing lycium barbarum polysaccharide provided by the invention has high purity, white color, excellent quality and high extraction rate which can reach more than 75%, has good effect of protecting liver, and can be used for developing medicines, health-care foods or special medical formula foods for protecting liver, alcoholic liver injury and chemical liver injury.
The above description is only an embodiment of the present invention, and not intended to limit the scope of the present invention, and all equivalent modifications made by the present invention in the specification or directly or indirectly applied to the related technical field are included in the scope of the present invention.
Claims (10)
1. A preparation method of a wolfberry extract containing wolfberry polysaccharide is characterized by comprising the following steps:
(1) micronizing fructus Lycii to obtain fructus Lycii fine powder;
(2) adding 8-12 times of water by weight into the medlar fine powder, and adding vitamin C to obtain a mixture;
(3) adding enzyme into the mixture for ultrasonic extraction to obtain an enzymolysis extracting solution; the enzyme is a mixed enzyme consisting of cellulase, pectinase and papain;
(4) centrifuging the enzymolysis extracting solution, and collecting supernatant;
(5) and (3) carrying out ultrafiltration separation on the supernatant by using an ultrafiltration membrane system to obtain a product with a molecular weight of 10-50kDa, and freeze-drying the product to obtain freeze-dried powder, namely the lycium barbarum extract containing lycium barbarum polysaccharide.
2. The method for preparing the lycium barbarum extract containing lycium barbarum polysaccharide according to claim 1, wherein the step (1) is specifically as follows: pulverizing fructus Lycii, drying at 40-50 deg.C, and micronizing the coarse powder in micronizer to obtain 300-400 mesh fructus Lycii fine powder.
3. The method for preparing the lycium barbarum extract containing lycium barbarum polysaccharides according to claim 1, wherein the step (2) further comprises: the mixture is rapidly cooled to-20 to-16 ℃, frozen for 2 to 4 hours and then rapidly thawed by microwave, and the microwave power is 3500 and 5000W.
4. The process of claim 3, wherein the step of rapidly cooling to-20 to-16 ℃ and freezing for 2-4 hours followed by rapid microwave thawing is repeated for 3 times.
5. The method of claim 4, wherein the "rapid cooling to-20 to-16 ℃ C" means that the temperature is reduced to-20 to-16 ℃ C within 1-3 min.
6. The method for preparing the lycium barbarum extract containing lycium barbarum polysaccharide according to claim 1, wherein the step (2) is specifically as follows: adding 8-12 times of water into the fructus Lycii fine powder, adding vitamin C and edible acid, and treating for 30-60min to obtain mixture.
7. The method for preparing the lycium barbarum extract containing lycium barbarum polysaccharide according to claim 1, wherein the vitamin C is added in an amount of 1.5-3g/L, the mass concentration of the edible acid in the mixture after the edible acid is added is 3-5%, and the edible acid is acetic acid.
8. The method for preparing the lycium barbarum extract containing lycium barbarum polysaccharide according to claim 1, wherein the addition amount of the enzyme is 1-3% of the mixture by mass, and the addition mass ratio of the cellulase, the pectinase and the papain is 1:2: 3.
9. The method for preparing the lycium barbarum extract containing lycium barbarum polysaccharide according to claim 1, wherein the ultrasonic extraction conditions are as follows: the temperature is 20-40 ℃; the ultrasonic power is 500-1700W; the circulating stirring speed is 500-2000 rpm; duty ratio 1: 1-1: 3; ultrasonic extraction time is 0.5-2 h.
10. The method for preparing the lycium barbarum extract containing lycium barbarum polysaccharide according to claim 1, wherein the step (4) is specifically as follows: centrifuging the enzymolysis extracting solution at 4000-.
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|---|---|---|---|---|
| CN103333268A (en) * | 2013-07-23 | 2013-10-02 | 草本创想生物科技（成都）有限公司 | Method for preparing lycium ruthenicum polysaccharide |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103333268A (en) * | 2013-07-23 | 2013-10-02 | 草本创想生物科技（成都）有限公司 | Method for preparing lycium ruthenicum polysaccharide |
| CN108456257A (en) * | 2017-02-22 | 2018-08-28 | 天津延生园生物科技有限公司 | A kind of extraction and purification process of high-purity Chinese yam polysaccharide |
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