CN113751087A - Chip connecting device - Google Patents
Chip connecting device Download PDFInfo
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- CN113751087A CN113751087A CN202110835328.5A CN202110835328A CN113751087A CN 113751087 A CN113751087 A CN 113751087A CN 202110835328 A CN202110835328 A CN 202110835328A CN 113751087 A CN113751087 A CN 113751087A
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
- B01L3/502715—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M1/00—Apparatus for enzymology or microbiology
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M1/00—Apparatus for enzymology or microbiology
- C12M1/34—Measuring or testing with condition measuring or sensing means, e.g. colony counters
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/02—Adapting objects or devices to another
- B01L2200/026—Fluid interfacing between devices or objects, e.g. connectors, inlet details
- B01L2200/027—Fluid interfacing between devices or objects, e.g. connectors, inlet details for microfluidic devices
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/06—Fluid handling related problems
- B01L2200/0684—Venting, avoiding backpressure, avoid gas bubbles
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/10—Integrating sample preparation and analysis in single entity, e.g. lab-on-a-chip concept
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/08—Geometry, shape and general structure
- B01L2300/0848—Specific forms of parts of containers
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/08—Geometry, shape and general structure
- B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2400/00—Moving or stopping fluids
- B01L2400/04—Moving fluids with specific forces or mechanical means
- B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
- B01L2400/0409—Moving fluids with specific forces or mechanical means specific forces centrifugal forces
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- Quick-Acting Or Multi-Walled Pipe Joints (AREA)
Abstract
A chip connecting device comprises a liquid pipeline and a liquid storage connecting chip. The liquid pipeline comprises a main body test tube and a mounting connector. The liquid storage connecting chip comprises a chip liquid storage plate, a connecting seat and a connecting joint. The chip liquid storage plate comprises a liquid storage plate main body, a chip main body and a liquid storage tank. The connecting seat comprises a connecting seat main body and a connecting hole. The liquid storage tank comprises a main flow tank, a flow dividing tank and an air outlet hole. One end of the connecting hole is communicated with the connecting joint, and the other end of the connecting hole is communicated with the main flow groove of the liquid storage groove. The extending direction of the splitter box and the included angle between the extending directions of the main runner are smaller than 90 degrees, and the air outlet is far away from one end head of the splitter box and is higher than the splitter box, so that liquid in the liquid storage connecting chip is prevented from flowing out of the air outlet. The chip connecting device is simple in structure, convenient to install and convenient to store liquid, reduces the labor intensity of operators, and improves the detection efficiency.
Description
Technical Field
The invention belongs to the technical field of medical equipment, and particularly relates to a chip connecting device.
Background
Real-time quantitative PCR is the most sensitive and reliable method for detecting gene expression. In the test process, the gene copy number in the sample can be reflected by monitoring the signal change of the fluorescent dye in real time, and the linear range of real-time quantitative PCR is wide, so that the gene with extremely low expression level and the gene with very high expression level in the same sample can be detected. The PCR chip is developed by a simple and optimized real-time quantitative PCR system, researchers can simultaneously research a large number of genes, the research on certain particularly interesting signal paths can be carried out, the chip can also be used as a method for verifying the chip result, the PCR chip can be widely applied to the fields of life science, biotechnology, disease detection, clinical medicine, environmental detection and the like, and the PCR chip is one of the research hotspots in the field of life science.
For example, a digital PCR chip and a method for preparing and using the same, which is disclosed in chinese patent No. CN201810027439.1, the PCR chip is used by dropping a PCR reaction solution from a sample inlet of a waterproof layer through a pipette, pumping air from a sample outlet of the waterproof layer through a hose connector by using an injector, and guiding the PCR reaction solution to flow into a main channel of a reaction layer until a part of the PCR reaction solution flows out from the sample outlet; seal cavity layer entry, use the syringe to outwards extract the air from cavity layer export until PCR reaction solution is full of the sprue and the independent PCR reaction chamber on reaction layer, can find out from this that take trouble in this testing process is hard, store inconveniently, increased the cost of labor, reduced detection efficiency.
Disclosure of Invention
In view of the above, the present invention provides a chip connection device convenient for storing liquid, so as to meet the industrial requirements.
A chip connecting device comprises a liquid pipeline for containing liquid and a liquid storage connecting chip arranged on the liquid pipeline. The liquid pipeline includes a main part test tube to and at least one setting is in the attach fitting of main part test tube one end. The liquid storage connecting chip comprises a chip liquid storage plate, a connecting seat arranged on the chip liquid storage plate, and a connecting joint arranged on the connecting seat. The chip liquid storage plate comprises a liquid storage plate main body, a chip main body and a liquid storage tank, wherein the chip main body is arranged on one side of the liquid storage plate main body, and the liquid storage tank is arranged on one side, close to the chip main body, of the liquid storage plate main body. The connecting seat comprises a connecting seat main body and a connecting hole arranged on the connecting seat main body. The liquid storage tank comprises a main flow tank connected to the connecting hole, at least one flow dividing tank arranged at one end, far away from the connecting hole, of the main flow tank, and at least one air outlet hole arranged at one end, far away from the main flow tank, of the flow dividing tank. One end of the connecting hole is communicated with the connecting joint, and the other end of the connecting hole is communicated with the main flow groove of the liquid storage groove. The extending direction of the splitter box and the included angle between the extending directions of the main runner are smaller than 90 degrees, and the air outlet is far away from one end head of the splitter box and is higher than the splitter box, so that liquid in the liquid storage connecting chip is prevented from flowing out of the air outlet.
Further, the liquid storage tank further comprises hot-melt solid arranged in the main flow tank or the sub-flow tank, and the hot-melt solid is one of solid paraffin and solidified grease.
Further, the main flow groove comprises a main flow groove head and one or more independent liquid channels arranged on the main flow groove head, each independent liquid channel comprises at least one chamber and microflow channels arranged among the chambers, and when the liquid storage connecting chip is not used, the independent liquid channels are not communicated with each other except the main flow groove head.
Further, the liquid pipeline still include at least one set up in the erection joint in insert establish the pipe to and a first stopper of setting on the main part test tube, insert establish the pipe with the center pin coincidence of erection joint, the external diameter of inserting and establishing the pipe is less than erection joint's internal diameter, just the external diameter of inserting and establishing the pipe equals the internal diameter of attach fitting.
Furthermore, the extending direction of the first limiting block is tangent to one side of the mounting joint.
Further, the liquid storage connecting chip is further provided with a second limiting block arranged on the connecting seat, the second limiting block is close to one side edge of the connecting joint to form an arc shape, the circle center of the arc coincides with the central axis of the connecting joint, the radius of the arc is equal to the outer diameter of the mounting joint, when the liquid storage connecting chip is mounted on the liquid pipeline, the second limiting block abuts against the first limiting block, and the extending direction of the first limiting block is perpendicular to the chip liquid storage plate.
Further, the central axis direction of the connection joint coincides with the extending direction of the chip liquid storage plate.
Further, in a cross section along a central axis of the connection joint, a height of the connection joint is greater than a height of the chip liquid storage plate.
Further, the air outlet is communicated with the connecting joint or the air outlet penetrates through the liquid storage plate main body.
Further, the connecting seat still includes that one sets up the stock solution hole of connecting hole one end, one sets up stock solution hole one side and intercommunication the liquid groove of abandoning in stock solution hole to and at least one setting is in abandon the exhaust hole of liquid groove one side, the one end intercommunication in exhaust hole abandons the cistern, and the other end intercommunication attach fitting.
Compared with the prior art, the chip connecting device provided by the invention has the advantages that the liquid in the liquid pipeline is transferred into the liquid storage connecting chip by connecting one end of the liquid pipeline with the liquid storage connecting chip. The connecting joint of the liquid storage connecting chip is connected to the mounting joint of the liquid pipeline, the liquid storage tank is arranged on the chip liquid storage plate of the liquid storage connecting chip, one end of the connecting hole is communicated with the connecting joint, the other end of the connecting hole is communicated with the liquid storage tank, and therefore liquid in the liquid pipeline is transferred to the liquid storage tank. The liquid storage tank stores liquid through the main flow groove and the flow dividing groove, and the flow dividing groove is far away from the air outlet hole formed in one end of the main flow groove, so that gas in the main flow groove and the flow dividing groove is discharged. In addition, the extending direction of flow distribution groove with contained angle between the extending direction of mainstream groove is less than 90 degrees, just venthole extending direction with stock solution board main part extending direction mutually perpendicular or become the contained angle, it can be thought that the venthole is kept away from the one end of chip main part is higher than the reservoir, the liquid pipeline and the stock solution connection chip prevents at the in-process that centrifugal separation handled the liquid in the stock solution connection chip is followed the venthole flows the stock solution connection chip outside. The chip connecting device is simple in structure, convenient to install and convenient to store liquid, reduces the labor intensity of operators, and improves the detection efficiency.
Drawings
Fig. 1 is a schematic structural diagram of a chip connection device provided in the present invention.
Fig. 2 is a schematic cross-sectional structure view of the chip connection device of fig. 1.
FIG. 3 is a schematic cross-sectional view of one embodiment of the connection socket in the liquid storage connection chip of FIG. 1.
Fig. 4 is a schematic cross-sectional view of a first embodiment of a liquid storage connection chip in the chip connection device of fig. 1.
Fig. 5 is a schematic cross-sectional view of a second embodiment of a liquid storage connection chip in the chip connection device of fig. 1.
Detailed Description
Specific examples of the present invention will be described in further detail below. It should be understood that the description herein of embodiments of the invention is not intended to limit the scope of the invention.
Fig. 1 to 4 are schematic structural diagrams of a chip connection device according to the present invention. The chip connection device includes a liquid pipe 10 for containing liquid (not shown), and a liquid storage connection chip 20 disposed on the liquid pipe 10. The chip connection device also includes other functional modules, such as assembly components, etc., which should be known to those skilled in the art, and will not be described in detail herein.
The main body test tube 11 is used for accommodating a liquid to be detected, and because the structure of the main body test tube 11 is large, only a part of the structure of the main body test tube 11 is shown in the figure, and the main body test tube 11 is a prior art and is not described herein again.
The erection joint 12 sets up the one end of main part test tube 11, erection joint 12 with main part test tube 11 communicates each other, all set up one in the erection joint 12 insert and establish pipe 13, insert establish pipe 13 with the center pin coincidence of erection joint 12, it can be thought that, the liquid accessible in the main part test tube 11 insert and establish pipe 13 and flow.
The outer diameter of the insertion tube 13 is smaller than the inner diameter of the installation joint 12, and it is conceivable that there is a gap between the insertion tube 13 and the installation joint 12 to facilitate the connection between the liquid pipeline 10 and the liquid storage connecting chip 20, and the installation joint 12 and the insertion tube 13 will be described in detail in conjunction with the liquid storage connecting chip 20.
In addition, when the liquid pipeline 10 is connected to the liquid storage connecting chip 20, the first stopper 14 is used to limit the position of the liquid storage connecting chip 20, and the first stopper 14 will be described in detail with reference to the liquid storage connecting chip 20.
The liquid storage connection chip 20 includes a chip liquid storage plate 21, a connection seat 22 disposed on the chip liquid storage plate 21, a connection joint 23 disposed on the connection seat 22, and a second stopper 24 disposed on the connection seat 22.
The chip liquid storage plate 21 comprises a liquid storage plate main body 211, a chip main body 212 arranged on one side of the liquid storage plate main body 211, and a liquid storage tank 213 arranged on one side of the liquid storage plate main body 211 close to the chip main body 212.
The chip main body 212 can contact with the liquid in the reservoir 213, and the chip main body 212 is a prior art and is not described herein again.
The liquid storage tank 213 includes a main flow tank 2131 disposed on the liquid storage plate main body 211 and connected to the following connecting hole 222, at least one branch flow tank 2132 disposed at an end of the main flow tank 2131 away from the following connecting hole 222, at least one air outlet hole 2133 disposed at an end of the branch flow tank 2132 away from the main flow tank 2131, and hot-melt solids (not shown) disposed in the main flow tank 2131 or the branch flow tank 2132.
The main flow channel comprises a main flow channel head (not shown), and one or more independent liquid channels (not shown) arranged on the main flow channel head, wherein each independent liquid channel comprises at least one chamber (not shown), and a micro-flow channel (not shown) arranged among a plurality of chambers. It is conceivable that a plurality of the chambers are connected in series with each other via the microfluidic channels to form the independent liquid channels, thereby performing liquid transfer and storage. In addition, when the reservoir connection chip 21 is not in use, the independent liquid channels are not communicated with each other except at the main flow channel end, which will be described in detail in conjunction with the hot-melt solid. One end of the main flow channel 2131 is connected to the connection seat 22, the liquid storage connection chip 20 transfers the liquid in the liquid pipeline 10 to the main flow channel 2131 through the connection joint 23 and the connection seat 22, and the main flow channel 2131 will be described in detail with reference to the connection seat 22.
The flow dividing groove 2132 is connected to one end of the main groove 2131, and the flow dividing groove 2132 connects the main groove 2131 and the gas outlet holes 2133 to each other, and it is conceivable that liquid may flow into the flow dividing groove 2132 along the main groove 2131, and that gas in the flow dividing groove 2132 and the main groove 2131 may flow in and out along the gas outlet holes 2133.
The air outlet hole 2133 is formed through the liquid storage plate main body 211, and it is conceivable that air in the liquid storage tank 213 can flow to the outside of the liquid storage plate main body 211 through the air outlet hole 2133, so as to facilitate the flow of liquid in the liquid storage tank 213. The end of the air outlet 2133, which is away from the shunting groove 2132, is higher than the shunting groove 2132, so as to prevent the liquid in the liquid storage connecting chip 20 from flowing out of the air outlet 2133, and it is conceivable that the extending direction of the air outlet 2133 and the extending direction of the liquid storage plate main body 211 may be perpendicular to each other or form an angle. In addition, an included angle between the extending direction of the branch flow groove 2132 and the extending direction of the main flow groove 2131 is smaller than 90 degrees, when the liquid in the liquid pipeline 10 is transferred into the liquid storage tank 213, the liquid pipeline 10 and the liquid storage connecting chip 20 are both subjected to centrifugal separation, the liquid is processed by rotating the liquid pipeline 10, and the liquid is transferred into the liquid storage tank 213 by utilizing the centrifugal force generated by rotating the liquid pipeline 10, and the included angle between the extending direction of the branch flow groove 2132 and the extending direction of the main flow groove 2131 is smaller than 90 degrees, so that the liquid contained in the liquid storage tank 213 is concentrated at the connecting position of the main flow groove 2131 and the branch flow groove 2132 under the centrifugal separation effect of the liquid storage connecting chip 20, and the liquid is prevented from flowing to the air outlet hole 2133 along the branch flow groove 2132. In an embodiment of the present application, included angles between extension directions of the main flow channel 2131 and the flow dividing channel 2132 are all 0 degree, that is, extension directions of the main flow channel 2131 and the flow dividing channel 2132 are parallel to each other.
The hot-melt solid is a substance which can be liquefied after being heated, the hot-melt solid is one of solid paraffin and solidified grease, the hot-melt solid can be arranged in the main flow channel 2131, the sub-flow channel 2132 or between the independent liquid channels of the main flow channel 2131, in this embodiment, the hot-melt solid is arranged between the independent liquid channels, and when the liquid storage connecting chip 20 is not used, the hot-melt solid is arranged between the independent liquid channels and is solid, so that the independent liquid channels except the end of the main flow channel are not communicated, and thus each independent liquid channel can independently distribute liquid. When the liquid storage connection chip 20 is in use, the chip main body 212 heats the hot melt solid and makes the hot melt solid become liquid, so as to conduct between the independent liquid channels.
In the invention, the liquid storage tank 213 and the air outlet hole 2133 have two embodiments, and in the first embodiment, the outer contour of the main flow tank 2131 and the outer contour of the branch flow tank 2132 which are connected together are in a U shape, so that the space of the liquid storage tank 213 in the liquid storage plate main body 211 is saved. In addition, the air outlet hole 2133 is arranged at one end of the branch flow groove 2132 far away from the main flow groove 2131, and the air outlet hole 2133 is arranged on the liquid storage plate main body 211 in a penetrating manner, so that air in the liquid storage tank 213 can be conveniently discharged through the air outlet hole 2133, and the liquid can be stored in the liquid storage tank 213.
In the second embodiment of the liquid storage tank 213 and the air outlet holes 2133, there are two of the branch tanks 2132, the air outlet holes 2133 are communicated with the connecting joint 23, and the outer contours of the main flow tank 2131 and the branch tanks 2132 connected together are M-shaped, so that the amount of the liquid stored in the liquid storage tank 213 is increased. The air outlet 2133 is communicated with the connecting joint 23, so that during the liquid storage process of the liquid storage tank 213, the air in the liquid storage tank 213 can flow into the connecting joint 23, so that the air between the liquid storage tank 213 and the connecting joint 23 flows back and forth, thereby facilitating the liquid storage in the liquid storage tank 213.
The connecting holder 22 includes a connecting holder body 221, and a connecting hole 222 provided on the connecting holder body 221. One end of the connection hole 222 communicates with the connection joint 23, and the other end of the connection hole 222 communicates with the main flow tank 2131 of the reservoir tank 213, and it is conceivable that the liquid in the liquid pipe 10 enters the reservoir tank 213 through the connection joint 23 and the connection hole 222 when transferred into the reservoir connection chip 20.
As shown in fig. 3, which is a schematic cross-sectional view of an embodiment of the connection seat 22 in the liquid storage connection chip 20, the connection seat 22 further includes a liquid storage hole 223 disposed at an end of the connection hole 222, a liquid discarding groove 224 disposed at one side of the liquid storage hole 223 and connected to the liquid storage hole 223, and at least one air vent 225 disposed at one side of the liquid discarding groove 224. One end of the exhaust hole 225 is communicated with the waste liquid tank 224, and the other end is communicated with the connecting joint 23, so that in the process of storing liquid in the waste liquid tank 224, air in the waste liquid tank 224 can flow into the connecting joint 23, and gas between the waste liquid tank 224 and the connecting joint 23 flows back and forth, so that liquid can be stored in the waste liquid tank 224 conveniently.
When the connection joint 23 transfers the liquid into the reservoir 213, the liquid storage hole 223 and the waste liquid tank 224 are used for storing the liquid, the liquid storage hole 223 is used for storing the liquid flowing into the reservoir 213, and the waste liquid tank 224 is used for collecting the excess liquid exceeding the capacity of the reservoir 213. When the liquid storage connection chip 20 has two rotational separation speeds during the centrifugal separation process, at the first rotational separation speed, since the diameter of the connection hole 222 is small, liquid cannot flow into the liquid storage tank 213, and the connection joint 23 transfers the liquid to the liquid storage hole 223 and the waste liquid tank 224. At the second rotational separation speed, the liquid in the liquid storage hole 223 enters the liquid storage tank 213, and it is conceivable that the liquid in the liquid storage hole 223 is the liquid entering the liquid storage tank 213, so that the control of the amount of the liquid entering the liquid storage tank 213 is achieved.
The connection joint 23 is used for connecting to the installation joint 12, so that the liquid pipeline 10 and the liquid storage connection chip 20 are integrated, and the installation joint 12 and the connection joint 23 can be connected through any one of a threaded connection mode and a snap connection mode. In this embodiment, the mounting adapter 12 and the connecting adapter 23 are connected by threads, that is, the mounting adapter 12 and the connecting adapter 23 are all threaded connecting pipes.
The inner diameter of the installation joint 12 is equal to the outer diameter of the connection joint 23, the outer diameter of the insertion pipe 13 is equal to the inner diameter of the connection joint 23, when the liquid pipeline 10 and the liquid storage connection chip 20 are installed together, the connection joint 23 is arranged between the insertion pipe 13 and the installation joint 12, and the insertion pipe 13 is inserted into the connection joint 23, so that the installation between the liquid pipeline 10 and the liquid storage connection chip 20 is stable. And the central axis direction of the connection tab 23 coincides with the extending direction of the chip liquid storage plate 21 so that the liquid in the connection tab 23 flows into the chip liquid storage plate 21. The height of the connection joint 23 is greater than the height of the chip reservoir plate 21 in a cross section along the central axis of the connection joint 23 to prevent the liquid in the chip reservoir plate 21 from flowing back into the connection joint 23.
The second limiting block 24 is in an arc shape near one side edge of the connecting joint 23, the center of the arc coincides with the central axis of the connecting joint 23, and the radius of the arc is equal to the outer diameter of the mounting joint 12, so that when the liquid storage connecting chip 20 is mounted on the liquid pipeline 10, the second limiting block 24 is attached to one side of the liquid pipeline 10.
When the liquid storage connecting chip 20 is mounted on the liquid pipeline 10, the second stopper 24 abuts against the first stopper 14, and it is conceivable that the first stopper 14 and the second stopper 24 limit the position of the liquid storage connecting chip 20. In addition, the extending direction of the first limit block 14 is tangent to one side of the installation joint 12, and in this embodiment, the first limit block 14 is disposed in a vertical direction. Since the extending direction of the first stopper 14 is perpendicular to the chip reservoir plate 21, it is conceivable that the reservoir connection chip 20 is disposed in a horizontal direction, thereby facilitating the flow of liquid in the reservoir 213 of the reservoir connection chip 20.
Compared with the prior art, the chip connecting device provided by the invention has the advantages that the liquid in the liquid pipeline 10 is transferred into the liquid storage connecting chip 20 by connecting the liquid storage connecting chip 20 to one end of the liquid pipeline 10. The connection joint 23 of the liquid storage connection chip 20 is connected to the mounting joint 12 of the liquid pipeline 10, the liquid storage tank 213 is disposed on the chip liquid storage plate 21 of the liquid storage connection chip 20, one end of the connection hole 222 is communicated with the connection joint 23, and the other end of the connection hole 222 is communicated with the liquid storage tank 213, so that the liquid in the liquid pipeline 10 is transferred to the liquid storage tank 213. The liquid storage tank 213 stores liquid through the main flow tank 2131 and the branch flow tank 2132, and the branch flow tank 2132 is away from the air outlet 2133 arranged at one end of the main flow tank 2131, so that the gas in the main flow tank 2131 and the gas in the branch flow tank 2132 are discharged. In addition, an included angle between the extending direction of the branch flow groove and the extending direction of the main flow groove is smaller than 90 degrees, and the extending direction of the air outlet hole 2133 and the extending direction of the liquid storage plate main body 211 are perpendicular to each other or form an included angle, it is conceivable that one end of the air outlet hole 2133 away from the chip main body 212 is higher than the liquid storage groove 213, and the liquid pipeline 10 and the liquid storage connection chip 20 prevent the liquid in the liquid storage connection chip 20 from flowing out of the liquid storage connection chip 20 through the air outlet hole 2133 in the centrifugal separation process. The chip connecting device is simple in structure, convenient to install and convenient to store liquid, reduces the labor intensity of operators, and improves the detection efficiency.
The above description is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the scope of the present invention, and any modifications, equivalents or improvements that are within the spirit of the present invention are intended to be covered by the following claims.
Claims (10)
1. A chip connecting device is characterized in that: the chip connecting device comprises a liquid pipeline for containing liquid, and a liquid storage connecting chip arranged on the liquid pipeline, wherein the liquid pipeline comprises a main body test tube and at least one mounting joint arranged at one end of the main body test tube, the liquid storage connecting chip comprises a chip liquid storage plate, a connecting seat arranged on the chip liquid storage plate, and a connecting joint arranged on the connecting seat, the chip liquid storage plate comprises a liquid storage plate main body, a chip main body arranged on one side of the liquid storage plate main body, and a liquid storage tank arranged on one side of the liquid storage plate main body, which is close to the chip main body, the connecting seat comprises a connecting seat main body, a connecting hole arranged on the connecting seat main body, and the liquid storage tank comprises a main flow groove connected to the connecting hole, at least one sets up the sprue is kept away from the sprue the splitter box of connecting hole one end to and at least one sets up the splitter box is kept away from the venthole of sprue one end, the one end intercommunication of connecting hole joint, the other end intercommunication of connecting hole the sprue of reservoir, the extending direction of splitter box with contained angle between the extending direction of sprue is less than 90 degrees, the venthole is kept away from the one end head of splitter box is higher than the splitter box, in order to prevent liquid in the stock solution connection chip is followed the venthole flows.
2. The chip connection device according to claim 1, wherein: the liquid storage tank further comprises hot-melt solid arranged in the main flow tank or the sub-flow tank, and the hot-melt solid is one of solid paraffin and solidified grease.
3. The chip connection device according to claim 1, wherein: the main runner comprises a main runner head and one or more independent liquid channels arranged on the main runner head, each independent liquid channel comprises at least one chamber and a plurality of microflow channels arranged between the chambers, and when the liquid storage connecting chip is not used, the independent liquid channels are not communicated with each other except the main runner head.
4. The chip connection device according to claim 1, wherein: the liquid pipeline still include at least one set up in what the erection joint was interior insert establish the pipe to and a first stopper that sets up on the main part test tube, insert establish the pipe with the center pin coincidence of erection joint, the external diameter of inserting and establishing the pipe is less than erection joint's internal diameter, just the external diameter of inserting and establishing the pipe equals attach joint's internal diameter.
5. The chip connection arrangement as claimed in claim 4, wherein: the extending direction of the first limiting block is tangent to one side of the mounting joint.
6. The chip connection arrangement as claimed in claim 4, wherein: the liquid storage connecting chip is also provided with a second limiting block arranged on the connecting seat, the edge of one side, close to the connecting joint, of the second limiting block is arc-shaped, the circle center of the arc coincides with the central axis of the connecting joint, the radius of the arc is equal to the outer diameter of the mounting joint, when the liquid storage connecting chip is mounted on the liquid pipeline, the second limiting block is abutted to the first limiting block, and the extending direction of the first limiting block is perpendicular to the chip liquid storage plate.
7. The chip connection device according to claim 1, wherein: the central axis direction of the connecting joint is consistent with the extending direction of the chip liquid storage plate.
8. The chip connection device according to claim 1, wherein: and on the section along the central axis of the connecting joint, the height of the connecting joint is greater than that of the chip liquid storage plate.
9. The chip connection device according to claim 1, wherein: the air outlet is communicated with the connecting joint or the air outlet penetrates through the liquid storage plate main body.
10. The chip connection device according to claim 1, wherein: the connecting seat still includes that one sets up the stock solution hole of connecting hole one end, one sets up stock solution hole one side and intercommunication the liquid groove of abandoning in stock solution hole to and at least one setting is in abandon the exhaust hole of liquid groove one side, the one end intercommunication in exhaust hole abandons the cistern, the other end intercommunication attach fitting.
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| CN202110835328.5A CN113751087B (en) | 2021-07-23 | 2021-07-23 | Chip connecting device |
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| CN202110835328.5A CN113751087B (en) | 2021-07-23 | 2021-07-23 | Chip connecting device |
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Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20060239862A1 (en) * | 2005-04-20 | 2006-10-26 | Konica Minolta Medical & Graphic, Inc. | Testing chip and micro analysis system |
| US20110244522A1 (en) * | 2010-03-30 | 2011-10-06 | Tae Seok Seo | Rotational pcr equipment and pcr method using the same |
| US20150224501A1 (en) * | 2012-09-18 | 2015-08-13 | Cynvenio Biosystems, Inc. | Fluid reservoir |
| US20170036208A1 (en) * | 2014-03-07 | 2017-02-09 | National Research Council Of Canada | Centrifugal microfluidic chip control |
| CN207259494U (en) * | 2017-09-07 | 2018-04-20 | 杭州凯基科技有限公司 | Droplet particles carrier package chip structure |
| CN108373969A (en) * | 2018-01-11 | 2018-08-07 | 中国科学院上海微系统与信息技术研究所 | A kind of digital pcr chip and preparation method thereof and application method |
| CN207944100U (en) * | 2018-02-05 | 2018-10-09 | 领航基因科技（杭州）有限公司 | A kind of droplet type digital pcr balance pressure chip |
| CN111929460A (en) * | 2020-08-19 | 2020-11-13 | 河南科技大学 | Sampling, chip and liquid inlet control device suitable for microfluidic automatic detection |
| CN215924902U (en) * | 2021-07-23 | 2022-03-01 | 嘉兴医脉赛科技有限公司 | Chip connecting device |
-
2021
- 2021-07-23 CN CN202110835328.5A patent/CN113751087B/en active Active
Patent Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20060239862A1 (en) * | 2005-04-20 | 2006-10-26 | Konica Minolta Medical & Graphic, Inc. | Testing chip and micro analysis system |
| US20110244522A1 (en) * | 2010-03-30 | 2011-10-06 | Tae Seok Seo | Rotational pcr equipment and pcr method using the same |
| US20150224501A1 (en) * | 2012-09-18 | 2015-08-13 | Cynvenio Biosystems, Inc. | Fluid reservoir |
| US20170036208A1 (en) * | 2014-03-07 | 2017-02-09 | National Research Council Of Canada | Centrifugal microfluidic chip control |
| CN207259494U (en) * | 2017-09-07 | 2018-04-20 | 杭州凯基科技有限公司 | Droplet particles carrier package chip structure |
| CN108373969A (en) * | 2018-01-11 | 2018-08-07 | 中国科学院上海微系统与信息技术研究所 | A kind of digital pcr chip and preparation method thereof and application method |
| CN207944100U (en) * | 2018-02-05 | 2018-10-09 | 领航基因科技（杭州）有限公司 | A kind of droplet type digital pcr balance pressure chip |
| CN111929460A (en) * | 2020-08-19 | 2020-11-13 | 河南科技大学 | Sampling, chip and liquid inlet control device suitable for microfluidic automatic detection |
| CN215924902U (en) * | 2021-07-23 | 2022-03-01 | 嘉兴医脉赛科技有限公司 | Chip connecting device |
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