CN216024802U

CN216024802U - Fluid device and gene sequencer with same

Info

Publication number: CN216024802U
Application number: CN202122470054.2U
Authority: CN
Inventors: 卓夏田; 谭大喜; 曹明友; 莫金佺
Original assignee: MGI Tech Co Ltd
Current assignee: MGI Tech Co Ltd
Priority date: 2021-10-13
Filing date: 2021-10-13
Publication date: 2022-03-15
Anticipated expiration: 2031-10-13

Abstract

The utility model provides a fluid device and a gene sequencer with the same, wherein the fluid device comprises: a container assembly; the reaction tank is communicated with the container assembly through a pipeline, and a flow passage is arranged in the reaction tank; the liquid pumping device is communicated with the reaction tank through a pipeline; the casing is provided with the first chamber that holds, the second that mutually independent holds the chamber and the third chamber that holds in the casing, and the container subassembly sets up in the first chamber that holds, and the reaction tank setting holds in the chamber at the second, and the pump liquid device sets up in the third holds the chamber. The technical scheme of the application can effectively solve the problem that the mutual influence among all parts is caused by unreasonable internal layout of the fluid device in the related art.

Description

Fluid device and gene sequencer with same

Technical Field

The utility model relates to the technical field of gene sequencing, in particular to a fluid device and a gene sequencer with the fluid device.

Background

In the field of gene sequencing, gene sequencers include fluidic devices for introducing reagents in container assemblies into sample containers (e.g., reaction cells) for chemical reaction with DNA samples to analyze the base sequence of specific DNA fragments. The fluid device is internally provided with a pump body, and the function of introducing the reagent into the sample container is realized. The internal layout of the existing fluid device is not reasonable enough, and the problems that the accuracy of an optical system is influenced by vibration generated when a pump body works, and condensed water and steam are easily generated by a container assembly to influence electrical components exist.

SUMMERY OF THE UTILITY MODEL

The utility model mainly aims to provide a fluid device and a gene sequencer with the same, so as to solve the problem that the internal layout of the fluid device in the related art is unreasonable, and the parts are affected with each other.

In order to achieve the above object, according to one aspect of the present invention, there is provided a fluid device including: a container assembly; the reaction tank is communicated with the container assembly through a pipeline, and a flow passage is arranged in the reaction tank; the liquid pumping device is communicated with the reaction tank through a pipeline; the casing is provided with the first chamber that holds, the second that mutually independent holds the chamber and the third chamber that holds in the casing, and the container subassembly sets up in the first chamber that holds, and the reaction tank setting holds in the chamber at the second, and the pump liquid device sets up in the third holds the chamber.

Further, the fluidic device further comprises a selector valve disposed between the container assembly and the reaction cell, the selector valve being disposed within the second receiving chamber.

Further, the selector valve includes first selector valve and second selector valve, and fluidic device still includes draw liquid device and three way connection, and three way connection sets up between draw liquid device, first selector valve and second selector valve, still is provided with the fourth in the casing and holds the chamber, and draw liquid device sets up in the fourth holds the chamber, and the fourth holds the chamber and sets up between first holding chamber and third holding chamber.

Further, the container assembly comprises a first reagent container group communicated with the first selection valve and a second reagent container group communicated with the second selection valve, the three-way joint comprises a first interface, a second interface and a third interface, the first interface is communicated with the liquid pumping device, the selection valves comprise a plurality of second selection valves, the second selection valves are connected in series, and one of the second selection valves is communicated with the first selection valve through the three-way joint.

Further, a liquid extraction device is arranged at the downstream of the reaction tank, and the liquid extraction device can generate suction force so that the liquid in the flow channel of the reaction tank is sucked to the outside or a waste liquid container positioned at the downstream of the liquid extraction device.

Further, the container assembly is arranged at the upstream of the reaction tank, the liquid pumping device is arranged at the downstream of the reaction tank, the liquid pumping device is a negative pressure pump, and the negative pressure pump can generate negative pressure so that liquid in the container assembly is sucked into the flow channel of the reaction tank.

Further, be equipped with many runners in the reaction tank, every runner is equipped with inlet and liquid outlet, the container subassembly passes through the pipe connection inlet, the pump liquid device passes through the pipe connection liquid outlet, liquid can flow into the runner and flow out from the liquid outlet from the inlet, the pipeline of connecting container subassembly and inlet includes trunk line and many reposition of redundant personnel pipelines, the selector valve is connected to trunk line's first end, many reposition of redundant personnel pipelines's first end assembles the second end that is connected to trunk line, many reposition of redundant personnel pipelines's second end is connected the inlet of many runners respectively.

Furthermore, one or more flow channels are arranged in the reaction tank, the number of the liquid pumping devices is the same as that of the flow channels, and each liquid pumping device is correspondingly connected with one flow channel.

Further, the fluid device further comprises a condensation pump, and the condensation pump is arranged in the first accommodating chamber.

Further, the fluid device also comprises a waste liquid container, and the liquid pumping device, the liquid pumping device and the condensing pump are communicated with the waste liquid container.

According to another aspect of the present invention, there is provided a gene sequencer comprising the fluidic device described above.

By applying the technical scheme of the utility model, the fluid device comprises: container subassembly, reaction tank, pump liquid device and casing. Container subassembly, reaction tank and pump liquid device all set up in the casing, can hold multiple reagent in the container subassembly, and the reaction tank passes through pipeline and container subassembly intercommunication, and the reagent can flow into in the reaction tank and take place the sequencing reaction with the DNA sample of treating the assay via the pipeline, and the back waste liquid is arranged away after the reaction finishes, then introduces next reagent, gets into next round of sequencing reaction. The first chamber, the second that hold of casing inside holds and the third holds and hold the chamber between mutually independent setting, and the pump liquid device that can produce the vibration sets up and holds the chamber at the third in the working process, and the normal work of other indoor parts just can not be influenced to the pump liquid device like this, especially can not influence the second and hold the indoor part of chamber. Simultaneously, because the container subassembly usually needs cold-stored heat preservation, set up the container subassembly alone in first holding the cavity, can effectively avoid the normal work that the condensate water and the steam that the container subassembly produced influence the electrical component in other cavities. In this application, set up a plurality of mutually independent cavities and will probably produce the part of mutual influence and place respectively in different cavities at casing inside, set up like this and can solve the interior overall arrangement of fluidic device among the correlation technique unreasonable problem that makes mutual influence between each part effectively, guaranteed the reliability of instrument.

Drawings

The accompanying drawings, which are incorporated in and constitute a part of this application, illustrate embodiments of the utility model and, together with the description, serve to explain the utility model and not to limit the utility model. In the drawings:

fig. 1 shows a schematic structural view of an embodiment of a fluidic device according to the present invention.

Wherein the figures include the following reference numerals:

2. a reaction tank; 3. a container assembly; 4. a liquid pumping device; 5. a selector valve; 6. a liquid extraction pipeline; 7. a fluid extraction assembly; 8. a first selector valve; 9. a second selector valve; 10. a three-way joint; 11. a first reagent container group; 12. a second reagent container set; 13. a flow channel; 14. a liquid inlet; 15. a liquid outlet; 16. a diversion pipeline; 17. a housing; 18. a first accommodation chamber; 19. a second accommodation chamber; 20. a third accommodation chamber; 21. a fourth accommodation chamber; 22. a condensate pump; 23. a liquid pumping device; 24. a waste liquid container.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. The following description of at least one exemplary embodiment is merely illustrative in nature and is in no way intended to limit the utility model, its application, or uses. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

It is noted that the terminology used herein is for the purpose of describing particular embodiments only and is not intended to be limiting of example embodiments according to the present application. As used herein, the singular forms "a", "an" and "the" are intended to include the plural forms as well, and it should be understood that when the terms "comprises" and/or "comprising" are used in this specification, they specify the presence of stated features, steps, operations, devices, components, and/or combinations thereof, unless the context clearly indicates otherwise.

The relative arrangement of the components and steps, the numerical expressions and numerical values set forth in these embodiments do not limit the scope of the present invention unless specifically stated otherwise. Meanwhile, it should be understood that the sizes of the respective portions shown in the drawings are not drawn in an actual proportional relationship for the convenience of description. Techniques, methods, and apparatus known to those of ordinary skill in the relevant art may not be discussed in detail but are intended to be part of the specification where appropriate. In all examples shown and discussed herein, any particular value should be construed as merely illustrative, and not limiting. Thus, other examples of the exemplary embodiments may have different values. It should be noted that: like reference numbers and letters refer to like items in the following figures, and thus, once an item is defined in one figure, further discussion thereof is not required in subsequent figures.

In the related art, the gene sequencer includes a fluid device, and a common fluid device is a fluid pumping device disposed upstream of a sample container (e.g., a reaction cell) for driving a fluid to flow in a positive pressure manner, which is liable to generate bubbles in the fluid and affect analysis results. Meanwhile, multiple reagents are required to be used during gene sequencing, the multiple reagents often share one pipeline, and when different reagents are switched to be used, one reagent is remained in the shared pipeline, so that the next reagent is polluted, and the analysis result is also influenced.

As shown in fig. 1, the fluid device of the present embodiment includes: container assembly 3, reaction cell 2, pumping means 4 and housing 17. Wherein, the reaction tank 2 is communicated with the container component 3 through a pipeline, and a flow passage 13 is arranged in the reaction tank 2; the liquid pumping device 4 is communicated with the reaction tank 2 through a pipeline; a first accommodating chamber 18, a second accommodating chamber 19 and a third accommodating chamber 20 which are independent from each other are arranged in the housing 17, the container assembly 3 is arranged in the first accommodating chamber 18, the reaction cell 2 is arranged in the second accommodating chamber 19, and the liquid pumping device 4 is arranged in the third accommodating chamber 20.

By applying the technical scheme of the embodiment, the fluid device comprises: container assembly 3, reaction cell 2, pumping means 4 and housing 17. Container module 3, reaction tank 2 and pump liquid device 4 all set up in casing 17, can hold multiple reagent in the container module 3, and reaction tank 2 passes through pipeline and container module 3 intercommunication, and the reagent can flow into in reaction tank 2 and wait that the DNA sample of assay takes place the sequencing reaction via the pipeline, and the back waste liquid is discharged after the reaction finishes, then introduces next kind of reagent, gets into next round of sequencing reaction. The first accommodating chamber 18, the second accommodating chamber 19 and the third accommodating chamber 20 in the housing 17 are arranged independently of one another, and the liquid pumping device 4 which can vibrate during operation is arranged in the third accommodating chamber 20, so that the liquid pumping device 4 does not affect the normal operation of the components in other chambers, especially the components in the second accommodating chamber 19. Meanwhile, as the container assembly 3 generally needs to be refrigerated and insulated, the container assembly 3 is independently arranged in the first accommodating chamber 18, and the condition that condensed water and steam generated by the container assembly 3 influence the normal work of electrical components in other chambers can be effectively avoided. In the embodiment, a plurality of independent chambers are arranged in the housing 17, and components which may affect each other are respectively placed in different chambers, so that the problem that the components affect each other due to unreasonable internal layout of the fluid device in the related art can be effectively solved, and the reliability of the instrument is ensured.

It should be noted that the container assembly 3, the reaction cell 2, and the pumping device 4 are disposed in different chambers in the housing 17, but these components may be connected by pipes.

In this embodiment, an optical device (not shown in the figure) is further disposed in the second accommodating chamber 19, and since the optical device belongs to a precision instrument and has a very high requirement on the surrounding environment, the optical device and the liquid pumping device 4 are separately disposed in different chambers, so that the optical device is prevented from being affected by vibration during the liquid pumping process of the liquid pumping device 4.

As shown in fig. 1, in the present embodiment, the fluidic device further includes a selector valve 5, the selector valve 5 being disposed between the container assembly 3 and the reaction cell 2, the selector valve 5 being disposed in the second accommodation chamber 19. The selector valve 5 has a liquid inlet and a liquid outlet, and can select a specific outlet to communicate with the inlet, thereby achieving the effect of selecting different solutions. Meanwhile, the selection valve 5 and the reaction tank 2 are arranged in the same chamber, the distance between the two is shortened, and the requirement that the pipeline between the two is as short as possible can be met.

As shown in fig. 1, in the present embodiment, the selector valve 5 includes a first selector valve 8 and a second selector valve 9, the fluid device further includes a liquid drawing device 23 and a three-way joint 10, the three-way joint 10 is disposed between the liquid drawing device 23, the first selector valve 8 and the second selector valve 9, a fourth accommodation chamber 21 is further disposed in the housing 17, the liquid drawing device 23 is disposed in the fourth accommodation chamber 21, and the fourth accommodation chamber 21 is disposed between the first accommodation chamber 18 and the third accommodation chamber 20. Specifically, the fluid device of the present embodiment further includes an extraction line 6 disposed between the three-way joint 10 and the extraction device 23, and the extraction line 6 connects the extraction device 23 and the selector valve 5. The selection valve 5 can accurately and rapidly select and switch the reagents, and the multi-stage selection valve can improve the flexibility of selecting various reagents. When different reagents are switched to be used, one reagent is remained in the shared pipeline, so that the next reagent is polluted, and the analysis result is also influenced. The liquid pumping device 23 can pump out the residual reagent in the reaction tank 2, so that the residual reagent is prevented from influencing the subsequent analysis result, the reliability of the further analysis result is ensured, and the accuracy of gene sequencing is improved. The fourth accommodating chamber 21 is arranged between the first accommodating chamber 18 and the third accommodating chamber 20, the first accommodating chamber 18 and the third accommodating chamber 20 can be isolated, each component of the fluid device can work normally, and the fourth accommodating chamber 21 can be used for placing other pipelines and components. Of course, the pumping device 23 can also be placed in the third receiving chamber 20.

In this embodiment, the pumping device 23 is a self-priming pump, which has the advantages of high efficiency, low cost, etc. Of course, in other possible embodiments, the liquid pumping device may also be a pump body such as a peristaltic pump or a piston pump.

As shown in fig. 1, in the present embodiment, the container assembly 3 includes a first reagent container group 11 communicating with the first selector valve 8 and a second reagent container group 12 communicating with the second selector valve 9, the three-way joint 10 includes a first port, a second port and a third port, the first port communicates with the liquid pumping device 23, the selector valve 5 includes a plurality of second selector valves 9, and the plurality of second selector valves 9 are connected in series, and one of the plurality of second selector valves 9 communicates with the first selector valve 8 through the three-way joint 10. The plurality of second selector valves 9 are arranged, so that the flexibility of selecting a plurality of reagents can be improved.

In the present embodiment, the liquid pumping device 23 is disposed downstream of the reaction cell 2, and the liquid pumping device 23 can generate a suction force so that the liquid in the flow channel 13 of the reaction cell 2 is sucked to the outside or to the waste liquid container 24 located downstream of the liquid pumping device 23. The liquid pumping device 23 pumps the residual reagent in the reaction tank 2 into the waste liquid container 24, so that the influence of the residual reagent on the subsequent analysis result is avoided. The waste liquid container 24 can collect waste liquid in the reaction process, and after one waste liquid container 24 is fully loaded, the waste liquid container 24 is manually taken away to be poured or replaced by a new waste liquid container 24.

In the present embodiment, the liquid pumping device 4 includes an electromagnetic valve and a syringe pump, and the function of driving the liquid to flow downstream can be realized by the cooperation of the pipeline and the electromagnetic valve and the syringe pump.

The container assembly 3 is arranged at the upstream of the reaction tank 2, the liquid pumping device 4 is arranged at the downstream of the reaction tank 2, and the liquid pumping device 4 is a negative pressure pump which can generate negative pressure so that the liquid in the container assembly 3 is sucked into the flow channel 13 of the reaction tank 2. The container assembly 3 contains a plurality of reagents, the reagents flow into the reaction tank 2 to perform sequencing reaction with the DNA sample to be analyzed, waste liquid is discharged after the reaction is finished, and then the next reagent is introduced to enter the next sequencing reaction. The liquid pumping device 4 is a negative pressure pump and is arranged at the downstream of the reaction tank 2, and liquid is drawn by negative pressure to flow towards the downstream, so that the liquid flow rate is controllable, bubbles are not easy to generate, and the reliability of an analysis result is ensured.

As shown in fig. 1, in the present embodiment, one or more flow channels 13 are provided in the reaction tank 2, the number of the liquid pumping devices 4 is the same as the number of the flow channels 13, and each liquid pumping device 4 is correspondingly connected to one flow channel 13. Each liquid pumping device 4 can individually drive the liquid in the corresponding flow passage 13 to flow towards the liquid pumping device 4. Each flow channel 13 can independently perform sequencing reaction, is isolated from each other, and can be used for synchronous sequencing of different samples.

As shown in fig. 1, in this embodiment, a plurality of flow channels 13 are disposed in the reaction tank 2, each flow channel 13 is provided with a liquid inlet 14 and a liquid outlet 15, the container assembly 3 is connected to the liquid inlet 14 through a pipeline, the liquid pumping device 4 is connected to the liquid outlet 15 through a pipeline, liquid can flow into the flow channel 13 from the liquid inlet 14 and flow out from the liquid outlet 15, the pipeline connecting the container assembly 3 and the liquid inlet 14 includes a main pipeline and a plurality of branch pipelines 16, the selector valve 5 is connected to a first end of the main pipeline, first ends of the plurality of branch pipelines 16 are converged and connected to second ends of the main pipeline, and second ends of the plurality of branch pipelines 16 are respectively connected to the liquid inlets 14 of the plurality of flow channels 13. The plurality of flow dividing pipes 16 are provided so that the solution flowing through the selector valve 5 can enter the plurality of flow paths 13 for reaction, respectively, thereby improving efficiency.

As shown in fig. 1, in the present embodiment, the fluid device further includes a condensate pump 22, and the condensate pump 22 is disposed in the first accommodation chamber 18. The condensate pump 22 can drain away the condensate and the steam generated by the container assembly 3, and prevent the condensate and the steam from affecting the normal operation of other components.

As shown in fig. 1, in the present embodiment, the fluid device further includes a waste liquid container 24, and the liquid pumping device 4, the liquid pumping device 23 and the condensate pump 22 are all communicated with the waste liquid container 24. The waste liquid generated in the working process of the fluid device can be discharged into the waste liquid container 24 through a special pipeline, the waste liquid generated by the liquid pumping device 4, the liquid pumping device 23 and the condensing pump 22 is discharged into the waste liquid container 24, and the container is manually taken away after being fully loaded and is poured or replaced by a new container.

The fluid device also comprises a three-way joint communicated with the liquid pumping device 23, three interfaces of the three-way joint respectively form a liquid pumping assembly 7 together with the liquid pumping device 23, the waste liquid container 24 and the three-way joint 10, and the liquid pumping device 23 and the three-way joint communicated with the liquid pumping device 23.

The application also provides a gene sequencer comprising the fluidic device. The fluid device can effectively solve the problem that the internal layout of the fluid device in the related art is unreasonable, so that the parts are affected with each other, and the reliability of the instrument is ensured. Therefore, the gene sequencer having the above-described fluidic device also has the above-described advantages.

In the description of the present invention, it is to be understood that the orientation or positional relationship indicated by the orientation words such as "front, rear, upper, lower, left, right", "lateral, vertical, horizontal" and "top, bottom", etc. are usually based on the orientation or positional relationship shown in the drawings, and are only for convenience of description and simplicity of description, and in the case of not making a reverse description, these orientation words do not indicate and imply that the device or element being referred to must have a specific orientation or be constructed and operated in a specific orientation, and therefore, should not be considered as limiting the scope of the present invention; the terms "inner and outer" refer to the inner and outer relative to the profile of the respective component itself.

Spatially relative terms, such as "above … …," "above … …," "above … …," "above," and the like, may be used herein for ease of description to describe one device or feature's spatial relationship to another device or feature as illustrated in the figures. It will be understood that the spatially relative terms are intended to encompass different orientations of the device in use or operation in addition to the orientation depicted in the figures. For example, if a device in the figures is turned over, devices described as "above" or "on" other devices or configurations would then be oriented "below" or "under" the other devices or configurations. Thus, the exemplary term "above … …" can include both an orientation of "above … …" and "below … …". The device may be otherwise variously oriented (rotated 90 degrees or at other orientations) and the spatially relative descriptors used herein interpreted accordingly.

It should be noted that the terms "first", "second", and the like are used to define the components, and are only used for convenience of distinguishing the corresponding components, and the terms have no special meanings unless otherwise stated, and therefore, the scope of the present invention should not be construed as being limited.

The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention, and various modifications and changes may be made by those skilled in the art. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.

Claims

1. A fluidic device, comprising:

a container assembly (3);

the reaction tank (2) is communicated with the container assembly (3) through a pipeline, and a flow channel (13) is arranged in the reaction tank (2);

the liquid pumping device (4) is communicated with the reaction tank (2) through a pipeline;

the container comprises a shell (17), wherein a first accommodating chamber (18), a second accommodating chamber (19) and a third accommodating chamber (20) which are independent of each other are arranged in the shell (17), a container assembly (3) is arranged in the first accommodating chamber (18), a reaction tank (2) is arranged in the second accommodating chamber (19), and a liquid pumping device (4) is arranged in the third accommodating chamber (20).

2. Fluidic device according to claim 1, characterized in that it further comprises a selection valve (5), said selection valve (5) being arranged between said container assembly (3) and said reaction cell (2), said selection valve (5) being arranged within said second containment chamber (19).

3. A fluid device as claimed in claim 2, characterized in that said selection valve (5) comprises a first selection valve (8) and a second selection valve (9), said fluid device further comprising an extraction device (23) and a three-way joint (10), said three-way joint (10) being arranged between said extraction device (23), said first selection valve (8) and said second selection valve (9), said housing (17) further having a fourth housing chamber (21) arranged therein, said extraction device (23) being arranged within said fourth housing chamber (21), said fourth housing chamber (21) being arranged between said first housing chamber (18) and said third housing chamber (20).

4. A fluidic device according to claim 3, characterized in that the container assembly (3) comprises a first set of reagent containers (11) communicating with the first selection valve (8) and a second set of reagent containers (12) communicating with the second selection valve (9),

the three-way joint (10) comprises a first interface, a second interface and a third interface, the first interface is communicated with the liquid pumping device (23),

the selector valve (5) comprises a plurality of second selector valves (9), the second selector valves (9) are connected in series, and one of the second selector valves (9) is communicated with the first selector valve (8) through the three-way joint (10).

5. A fluidic device according to claim 3 or 4, characterized in that said pumping means (23) is arranged downstream of said reaction cell (2), said pumping means (23) being adapted to generate a suction force such that the liquid in the flow channel (13) of said reaction cell (2) is sucked to the outside or to a waste container (24) located downstream of said pumping means (23).

6. The fluidic device according to any one of claims 1 to 4, wherein the container assembly (3) is arranged upstream of the reaction cell (2), the pumping device (4) is arranged downstream of the reaction cell (2), and the pumping device (4) is a negative pressure pump capable of generating a negative pressure such that the liquid in the container assembly (3) is sucked into the flow channel (13) of the reaction cell (2).

7. A fluidic device according to any one of claims 2 to 4, characterized in that a plurality of said flow channels (13) are provided in said reaction chamber (2), each of said flow channels (13) being provided with a liquid inlet (14) and a liquid outlet (15), the container assembly (3) is connected with the liquid inlet (14) through a pipeline, the liquid pumping device (4) is connected with the liquid outlet (15) through a pipeline, liquid can flow into the flow channel (13) from the liquid inlet (14) and flow out from the liquid outlet (15), the pipeline connecting the container assembly (3) and the liquid inlet (14) comprises a main pipeline and a plurality of branch pipelines (16), the first end of main pipeline is connected selector valve (5), and is many the first end of reposition of redundant personnel pipeline (16) assembles and is connected to the second end of main pipeline, and is many the second end of reposition of redundant personnel pipeline (16) is connected many respectively the inlet (14) of runner (13).

8. A fluidic device according to any one of claims 1 to 4, wherein one or more flow channels (13) are provided in said reaction chamber (2), the number of said pumping means (4) is the same as the number of said flow channels (13), and one flow channel (13) is associated with each of said pumping means (4).

9. A fluidic device according to claim 3 or 4, characterized in that it further comprises a condensation pump (22), said condensation pump (22) being arranged inside said first containment chamber (18).

10. A fluidic device according to claim 9, characterized in that it further comprises a waste container (24), said pumping means (4), said extraction means (23) and said condensation pump (22) being in communication with said waste container (24).

11. A gene sequencer comprising the fluidic device of any one of claims 1-10.