CN218842172U

CN218842172U - Fluid system of gene sequencer

Info

Publication number: CN218842172U
Application number: CN202223528229.1U
Authority: CN
Inventors: 赵艳军; 刘胜举; 王雨田
Original assignee: Zhengzhou Sikun Biological Engineering Co ltd
Current assignee: Zhengzhou Sikun Biological Engineering Co ltd
Priority date: 2022-12-23
Filing date: 2022-12-23
Publication date: 2023-04-11
Anticipated expiration: 2032-12-23

Abstract

The present disclosure provides a fluidic system of a gene sequencer, the fluidic system comprising: the device comprises a valve bank, a row pump, a waste liquid module, a pipeline and a plurality of groups of chip modules; each group of the chip modules is respectively connected with the valve bank through the pipeline, and the parallel pump is respectively connected with the valve bank and the waste liquid module through the pipeline; the valve group comprises a plurality of electromagnetic valves, and the valve group is used for determining the current chip module in a working state in the plurality of groups of chip modules by utilizing the valve position of each electromagnetic valve; the current chip module is used for receiving a reagent, and carrying out reagent reaction based on the reagent to generate reagent reaction waste liquid; and the reagent reaction waste liquid is conveyed to the parallel pump through the valve group; the parallel pump is used for conveying the reagent reaction waste liquid to the waste liquid module. The gene sequencer comprising the fluid system can be miniaturized, has high flux and is used for freely combining chips.

Description

Fluid system of gene sequencer

Technical Field

The disclosure relates to the technical field of gene sequencers, in particular to a fluid system of a gene sequencer.

Background

The gene sequencer is also called DeoxyriboNucleic Acid (DNA) sequencer, and is an instrument for determining the base sequence, the type and the quantity of DNA fragments. It is generally applied to human genome sequencing, gene diagnosis of human genetic diseases, infectious diseases and cancers, paternity test and individual identification of forensic doctors, screening of bioengineering drugs, animal and plant crossbreeding, and the like.

With the diversification of the service requirements of the gene sequencer, the development trend of the gene sequencer is towards low cost, miniaturization, high throughput, free chip combination and the like, and therefore, the gene sequencer meeting the requirements is especially important.

SUMMERY OF THE UTILITY MODEL

In view of the above, the present disclosure provides at least one fluid system of a gene sequencer.

In a first aspect, the present disclosure provides a fluidic system of a gene sequencer, comprising: the system comprises a valve bank, a row pump, a waste liquid module, a pipeline and a plurality of groups of chip modules; each group of chip modules is respectively connected with the valve group through the pipeline, and the gang pump is respectively connected with the valve group and the waste liquid module through the pipeline;

the valve group comprises a plurality of electromagnetic valves, and the valve group is used for determining the current chip module in a working state in the plurality of groups of chip modules by utilizing the valve position of each electromagnetic valve;

the current chip module is used for receiving a reagent, and performing reagent reaction based on the reagent to generate reagent reaction waste liquid; and the reagent reaction waste liquid is conveyed to the parallel pump through the valve group;

the parallel pump is used for conveying the reagent reaction waste liquid to the waste liquid module.

In a possible embodiment, the method further comprises: a support module and a rotary valve; the support module is provided with a plurality of reagent boxes and reagent needles corresponding to the reagent boxes; each reagent needle is respectively connected with one port of the rotary valve; the rotary valve is connected with the plurality of groups of chip modules;

the reagent needle is used for puncturing a reagent port on the reagent box, sucking a reagent from the reagent box and conveying the sucked reagent to the current chip module through a port of the connected rotary valve.

In a possible embodiment, when each group of the chip modules is connected to the valve group through a plurality of the pipelines, the valve group is connected to the tandem pump through a plurality of the pipelines; the inline pump includes a plurality of injectors; the number of pipelines between the chip module and the valve group is consistent with the number of pipelines between the valve group and the gang pump, and the number of pipelines between the valve group and the gang pump is consistent with the number of injectors;

the injector is used for controlling the current chip module to convey the reagent reaction waste liquid to the valve group through a pipeline corresponding to the injector by pulling a piston on the injector;

the valve group is used for conveying the reagent reaction waste liquid to the injector of the parallel pump through a pipeline corresponding to the injector;

the injector is also used for pushing the reagent reaction waste liquid to the waste liquid module by pushing the piston on the injector.

In a possible embodiment, the method further comprises: a cleaning module; the cleaning module is connected with the combined pump through a pipeline;

the cleaning module is used for conveying the stored cleaning reagent to the row pump;

the inline pump is used for conveying the cleaning reagent into the injector and conveying the cleaning reagent to the waste liquid module by pushing the piston of the injector.

In a possible implementation manner, the valve group is further configured to determine a chip module to be cleaned in the plurality of groups of chip modules based on a valve position of each solenoid valve;

the inline pump, after receiving the wash reagent, is further to: and inputting the cleaning reagent to the chip module to be cleaned through the valve group.

In a possible embodiment, when the system comprises a rack module and a rotary valve, the chip module to be cleaned is further configured to deliver the cleaning reagent into the reagent cartridge of the rack module through the rotary valve when the cleaning reagent is received.

In a possible embodiment, the chip module at least comprises a liquid inlet, a liquid outlet and a temperature control module;

the chip module receives a reagent, and performs reagent reaction based on the reagent to generate reagent reaction waste liquid; and when the reagent reaction waste liquid is conveyed to the row pump through the valve group, the reagent reaction waste liquid is used for:

receiving a reagent from the inlet port; controlling the reaction temperature of the reagent by the temperature control module; and carrying out reagent reaction based on the reagent at the reagent reaction temperature to generate reagent reaction waste liquid, conveying the reagent reaction waste liquid to the valve group from the liquid outlet, and conveying the reagent reaction waste liquid to the gang pump through the valve group.

In one possible embodiment, the solenoid valve is a two-position three-way solenoid valve; each electromagnetic valve comprises a first communication port, a second communication port and a third communication port; the electromagnetic valve comprises a first valve position for indicating power on and a second valve position for indicating power off;

the plurality of groups of chip modules are respectively connected with the first communication port or the second communication port of the electromagnetic valve; and a third port of the electromagnetic valve is connected with the combined pump.

The embodiment of the disclosure provides a fluid system of a gene sequencer, wherein a plurality of groups of chip modules are arranged in the fluid system, each group of chip modules can be provided with a plurality of channels for outputting reagent reaction waste liquid, so that the flux of the gene sequencer is improved, and the gene sequencer meets the requirement of high flux; the chip module is connected with the valve group, the valve group comprises a plurality of electromagnetic valves, and the valve positions of the electromagnetic valves are used for determining the current chip module in a working state in the plurality of groups of chip modules, so that the function of freely combining the chips on the computer is realized, and the use flexibility of the gene sequencer is improved.

Meanwhile, the fluid system can control one chip module to be in a working state at a time through the valve group, so that independent sample introduction of each group of chip modules is realized, the valve group is simple and convenient to set, and the gene sequencer has the characteristics of low cost and miniaturization.

In order to make the aforementioned objects, features and advantages of the present disclosure more comprehensible, preferred embodiments accompanied with figures are described in detail below.

Drawings

In order to more clearly illustrate the technical solutions of the embodiments of the present disclosure, the drawings required in the embodiments will be briefly described below, and the drawings herein incorporated in and forming a part of the specification illustrate embodiments consistent with the present disclosure and, together with the description, serve to explain the technical solutions of the present disclosure. It is appreciated that the following drawings depict only certain embodiments of the disclosure and are therefore not to be considered limiting of its scope, for those skilled in the art will be able to derive additional related drawings therefrom without the benefit of the inventive faculty.

FIG. 1 is a schematic diagram of a fluidic system of a gene sequencer according to an embodiment of the present disclosure;

FIG. 2a is a schematic diagram showing a solenoid valve in a fluidic system of a gene sequencer according to an embodiment of the present disclosure;

FIG. 2b is a schematic diagram illustrating a connection relationship between a valve set and a chip module in a fluid system of a gene sequencer according to an embodiment of the disclosure;

FIG. 3 is a schematic diagram of the fluidic system of another gene sequencer provided in an embodiment of the present disclosure;

FIG. 4 is a schematic diagram illustrating a connection relationship between a valve set and a chip module in a fluid system of a gene sequencer according to an embodiment of the disclosure;

fig. 5 shows a schematic configuration diagram of a fluid system of another gene sequencer provided in the embodiments of the present disclosure.

Detailed Description

To make the objects, technical solutions and advantages of the embodiments of the present disclosure more apparent, the technical solutions in the embodiments of the present disclosure will be described clearly and completely with reference to the drawings in the embodiments of the present disclosure, and it is obvious that the described embodiments are only a part of the embodiments of the present disclosure, not all of the embodiments. The components of the embodiments of the present disclosure, as generally described and illustrated in the figures herein, could be arranged and designed in a wide variety of different configurations. Thus, the following detailed description of the embodiments of the present disclosure, presented in the figures, is not intended to limit the scope of the claimed disclosure, but is merely representative of selected embodiments of the disclosure. All other embodiments, which can be derived by a person skilled in the art from the embodiments of the disclosure without making creative efforts, shall fall within the protection scope of the disclosure.

With the diversification of the business requirements of gene sequencers, the development trend of gene sequencers is toward low cost, miniaturization, high throughput, chip free combination and computer-on-machine, and the like. Based on this, the embodiment of the present disclosure provides a fluid system of a gene sequencer, in which a plurality of groups of chip modules are arranged, so that the flux of the gene sequencer is increased, and the gene sequencer can meet the requirement of high flux; the chip module is connected with the valve group, the valve group comprises a plurality of electromagnetic valves, and the current chip module in a working state in the plurality of groups of chip modules is determined by utilizing the valve position of each electromagnetic valve, so that the function of freely combining the chips on the computer is realized, and the use flexibility of the gene sequencer is improved.

It should be noted that: like reference numbers and letters refer to like items in the following figures, and thus, once an item is defined in one figure, it need not be further defined and explained in subsequent figures.

For the purpose of understanding the embodiments of the present disclosure, a fluid system of a gene sequencer disclosed in the embodiments of the present disclosure will be described in detail. Referring to fig. 1, a schematic diagram of a fluidic system of a gene sequencer according to an embodiment of the present disclosure is shown, where the fluidic system includes: a valve block 500, a gang pump 600, a waste liquid module 700, a pipe 300 and a plurality of sets of chip modules 400; each group of the chip modules 400 is connected to the valve block 500 through the pipe 300, and the gang pump 600 is connected to the valve block 500 and the waste liquid module 700 through the pipe 300.

The valve group comprises a plurality of electromagnetic valves, and the valve group is used for determining the current chip module in a working state in the plurality of groups of chip modules by utilizing the valve positions of the electromagnetic valves.

The current chip module is used for receiving a reagent, and carrying out reagent reaction based on the reagent to generate reagent reaction waste liquid; and delivering the reagent reaction waste liquid to the gang pump through the valve bank.

During implementation, the current chip module in a working state in the plurality of groups of chip modules can be determined by adjusting the valve positions of the plurality of electromagnetic valves in the valve group. The valve positions of the solenoid valve comprise a first valve position indicating power-on and a second valve position indicating power-off. The relationship between the current chip module and the valve positions of the plurality of electromagnetic valves can be determined according to the connection relationship between each group of chip modules and the valve group.

In one mode, the solenoid valve is a two-position three-way solenoid valve; each electromagnetic valve comprises a first communication port, a second communication port and a third communication port; the electromagnetic valve comprises a first valve position for indicating power on and a second valve position for indicating power off; the plurality of groups of chip modules are respectively connected with the first communication port or the second communication port of the electromagnetic valve; and a third port of the electromagnetic valve is connected with the combined pump.

Referring to the schematic diagram of the solenoid valve shown in fig. 2a, the solenoid valve includes a first communication port 21, a second communication port 22, and a third communication port 23. The chip module is connected with the first communication port or the second communication port of each electromagnetic valve in the valve group, and the third communication port of each electromagnetic valve is connected with the parallel-row pump vector.

For example, referring to a schematic diagram of a connection relationship between the valve group and the chip module shown in fig. 2b, if the number of groups of the chip modules is 4, that is, the chip module 1, the chip module 2, the chip module 3, and the chip module 4, 3 solenoid valves may be included in the valve group, the solenoid valve 1, the solenoid valve 2, and the solenoid valve 3, wherein the chip module 1 is connected to the first communication port of the solenoid valve 1, the chip module 2 is connected to the second communication port of the solenoid valve 1, the chip module 3 is connected to the first communication port of the solenoid valve 2, the chip module 4 is connected to the second communication port of the solenoid valve 2, the third communication port of the solenoid valve 1 is connected to the first communication port of the solenoid valve 3, the third communication port of the solenoid valve 2 is connected to the second communication port of the solenoid valve 3, and the third communication port of the solenoid valve 3 is connected to the tandem pump.

See the mapping relationship between the valve positions of the solenoid valves and the working states of the chip modules shown in the following table 1.

TABLE 1 mapping relationship between valve positions of solenoid valves and operating states of chip modules

As can be seen from the above table, when the valve position of the solenoid valve 1 is the first valve position indicating power on G, the valve position of the solenoid valve 2 is the second valve position indicating power off T, and the valve position of the solenoid valve 3 is the first valve position indicating power on, the chip module 1 is in the operating state. When the valve position of the electromagnetic valve 1 is the second valve position indicating power loss, the valve position of the electromagnetic valve 2 is the second valve position indicating power loss, and the valve position of the electromagnetic valve 3 is the first valve position indicating power gain, the chip module 2 is in a working state. And then through the valve position of each solenoid valve in the valves group, realized the control to the operating condition of chip module.

In practice, the number of the solenoid valves in the valve set and the connection relationship between the solenoid valves and the chip module may be set as required, and this is only an exemplary illustration.

After the valve group determines the current chip module in a working state in the plurality of groups of chip modules by using the valve positions of the electromagnetic valves, the current chip module receives a reagent, and the reagent can be any reagent required by a gene sequencing process, and is not specifically limited here. The reagent received by the current chip module may be delivered from the reagent kit, or may be input to the current chip module in response to a manual operation.

The current chip module performs a reagent reaction based on the received reagent, generating a reagent reaction waste. The reagent reaction can be any reaction process existing in gene sequencing. The current chip module carries reagent reaction waste liquid to the valves in, and the valves carry the reagent reaction waste liquid to the gang pump. And then, the ally oneself with the row pump and carry reagent reaction waste liquid to the waste liquid module. The waste liquid module can be a waste liquid box for storing reagent reaction waste liquid.

When the device is implemented, the working of the row pump can drive the circulation of the reagent and the reagent reaction waste liquid in the fluid system. For example, the tandem pump may cause the reagent to flow through the current chip module by the movement of the built-in piston, and after generating the reagent reaction waste liquid, the reagent reaction waste liquid is conveyed to the valve set and then flows into the tandem pump. The parallel pump conveys the inflowing reagent reaction waste liquid to the waste liquid module again through the movement of the control piston.

The fluid system includes conduits therein that may be used to transport reagents and reagent reaction effluents. Each group of chip modules is connected with the valve group through a pipeline, the row-connecting pump is connected with the valve group through a pipeline, and the row-connecting pump is connected with the waste liquid module through a pipeline. The conduit may be used to transport any liquid present in the fluid system, such as reagents drawn into the fluid system, reagent reaction effluents produced, and the like.

For example, the reagent can be input into the current chip module through a pipeline, and after the reagent reaction waste liquid is generated by the current chip module, the reagent reaction waste liquid is input into the valve group through the pipeline, and the valve group conveys the reagent reaction waste liquid into the parallel pump through the pipeline. The parallel pump conveys the reagent reaction waste liquid into the waste liquid module through a pipeline.

In an alternative embodiment, as shown in fig. 3, the fluid system may further comprise: a support module 100 and a rotary valve 200; the rack module 100 is provided with a plurality of reagent boxes and reagent needles corresponding to each reagent box; each of the reagent needles is connected to one port of the rotary valve 200; the rotary valve 200 is connected to the plurality of sets of chip modules 400.

The support module is provided with a plurality of reagent kits, and the reagent kits are used for storing reagents required by the gene sequencing process. Each reagent kit is provided with a reagent needle, and each reagent needle is connected with one port of the rotary valve. Wherein the number of ports of the rotary valve is not less than the number of reagent cartridges in the rack module. And the reagent needle pierces a reagent port on the reagent box, sucks the reagent from the reagent box, inputs the sucked reagent to a port of the connected rotary valve, and then inputs the reagent to the current chip module through a common port of the rotary valve.

The support module is connected with the rotary valve through a pipeline, and the rotary valve is connected with each group of chip modules through a pipeline. When the chip module is implemented, after the reagent is sucked by the reagent needles on the support module, the reagent is conveyed to the rotary valve through the pipeline, and then the rotary valve conveys the reagent into the current chip module through the pipeline.

Here, through set up a plurality of reagent boxes on the support module, every reagent box links to each other with a port of rotary valve, can deposit multiple type reagent in the reagent box, can be more convenient extract the required various reagents of gene sequencing in-process from the support module, ensured the sequencing work of gene sequencer.

In an alternative embodiment, when each group of the chip modules is connected with the valve group through a plurality of the pipelines, the valve group is connected with the tandem pump through a plurality of the pipelines; the inline pump includes a plurality of injectors; the number of pipelines between the chip module and the valve group is consistent with the number of pipelines between the valve group and the gang pump, and the number of pipelines between the valve group and the gang pump is consistent with the number of injectors.

The injector is used for controlling the current chip module to convey the reagent reaction waste liquid to the valve group through a pipeline corresponding to the injector by pulling the piston on the injector. And the valve group is used for conveying the reagent reaction waste liquid to the injector of the parallel pump through a pipeline corresponding to the injector. The injector is also used for pushing the reagent reaction waste liquid to the waste liquid module by pushing the piston on the injector.

During implementation, each group of chip modules is connected with the valve group through N pipelines, the valve group is connected with the parallel pump through N pipelines, N injectors are arranged in the parallel pump, each injector is used for controlling one pipeline, a piston can be arranged in each injector, and liquid conveying in a fluid system is realized through pulling and pushing of the piston. Wherein N is a positive integer greater than 1.

If N is 4, the injector 1 controls the channel 1, the injector 2 controls the channel 2, the injector 3 controls the channel 3, and the injector 4 controls the channel 4. Illustratively, when the injector 1 pulls the piston, for example, the piston is pulled from inside to outside, the current chip module is controlled to input the reagent reaction waste liquid into the valve bank through the pipeline 1. The valve group conveys the reagent reaction waste liquid to the valve group through a pipeline 1. The valve group conveys the reagent reaction waste liquid into the injector 1 through the pipeline 1. Wherein the working processes of the injector 2, the injector 3 and the injector 4 are the same as the injector 1; the plurality of injectors can work synchronously or asynchronously.

For example, referring to fig. 4, the chip modules are 4 groups, and the number N of the channels is 4 for an exemplary description. The valve bank can include 12 solenoid valves, and the connection relationship of the solenoid valves and the connection relationship of the chip module and the solenoid valves are shown in fig. 4. In this case, the mapping relationship between the operating states of the chip modules and the valve positions of the solenoid valves is shown in table 2 below.

TABLE 2 mapping relationship between the working state of each group of chip modules and the valve position of the solenoid valve

When the control valve group is implemented, the chip module in a working state is controlled by controlling the valve position of each electromagnetic valve in the control valve group. For example, when the valve position of the solenoid valve 501 is G, the valve position of the solenoid valve 502 is G, the valve position of the solenoid valve 503 is G, the valve position of the solenoid valve 504 is G, the valve position of the solenoid valve 505 is T, the valve position of the solenoid valve 506 is T, the valve position of the solenoid valve 507 is T, the valve position of the solenoid valve 508 is T, the valve position of the solenoid valve 509 is G, the valve position of the solenoid valve 510 is G, the valve position of the solenoid valve 511 is G, and the valve position of the solenoid valve 512 is G, the chip module 401 is in a working state, that is, the sample is currently injected into the chip module 401.

For example, if the chip module 401 is a chip module in an operating state, the four syringes 601 on the parallel pump 600 pull the pistons synchronously, so that the chip module 401 receives the reagent, and performs reagent reaction based on the reagent to obtain reagent reaction waste liquid, the reagent reaction waste liquid is input into the valve group through 4 pipelines, the

solenoid valves

501, 502, 503, and 504 in the valve group input the reagent reaction waste liquid into the

solenoid valves

509, 510, 511, and 512, and then the

solenoid valves

509, 510, 511, and 512 input the reagent reaction waste liquid into the syringes of the parallel pump through 4 pipelines.

Here, the reagent reaction waste liquid can be transported through a plurality of pipelines, so that the reagent transport efficiency is improved, and the detection efficiency of the gene sequencer is further improved.

In an alternative embodiment, as shown in fig. 3, the fluid system further comprises: a cleaning module 800; the cleaning module 800 is connected to the inline pump 600 through a pipe 300.

The cleaning module 800 is configured to deliver the stored cleaning reagent to the tandem pump 600; the parallel pump 600 is configured to deliver the cleaning reagent into a syringe, and deliver the cleaning reagent to the waste liquid module 700 by pushing a piston of the syringe.

When the kit is implemented, a cleaning module can be arranged, the cleaning module can be a kit, and a cleaning reagent is stored in the kit. The cleaning module delivers the deposited cleaning reagent to the inline pump 600. For example, the injector can be controlled to pull the cleaning reagent from the cleaning module by pulling the piston of the injector, and then the cleaning reagent is conveyed into the waste liquid module 700 by pushing the piston of the injector, so that the cleaning of the pipeline between the cleaning module and the parallel pump, the cleaning of the pipeline between the waste liquid module and the parallel pump, and the cleaning of the injector can be completed.

Here, through setting up the washing module, can be faster through the realization of syringe to the washing of pipeline and syringe to carry out next reagent reaction process after wasing, alleviate the influence that residual liquid in pipeline or the syringe caused next reagent reaction, with the precision that improves gene sequencing.

In an optional embodiment, the valve group is further configured to determine a chip module to be cleaned in the plurality of groups of chip modules based on a valve position of each solenoid valve; the inline pump, after receiving the wash reagent, is further to: and inputting the cleaning reagent to the chip module to be cleaned through the valve group.

In practice, one cleaning process may determine one chip module to be cleaned, the determination process of the chip module to be cleaned is the same as the determination process of the current chip module, and the determination process of the chip module to be cleaned may refer to the above description of determining the current chip module, which is not described in detail herein.

The gang pump can input the cleaning reagent to the chip module to be cleaned through the valves after receiving the cleaning reagent to realize the cleaning of the pipelines between the valves and the gang pump, the cleaning of the pipelines between the valves and the chip module to be cleaned, and the cleaning of the chip module to be cleaned.

For example, the piston in the syringe of the tandem pump may be controlled to move from inside to outside (i.e., pull the piston), so that the cleaning reagent stored in the cleaning module is conveyed into the syringe through the pipeline, and the cleaning reagent in the syringe is input into the port a of the tandem pump through the port B of the tandem pump after moving from outside to inside (i.e., pushing the piston), and then enters the pipeline through the port a of the tandem pump, and then flows into the valve bank through the pipeline, and flows into the chip module to be cleaned through the valve bank.

The valve group and the chip module can be cleaned by utilizing a cleaning reagent, so that the cleaned chip module is utilized to perform next reagent reaction, the influence of residual liquid in the chip module on the next reagent reaction is relieved, and the accuracy of gene sequencing is improved.

In an alternative embodiment, when the system comprises a rack module and a rotary valve, the chip module to be cleaned is further configured to deliver the cleaning reagent into the reagent cartridge of the rack module through the rotary valve when the cleaning reagent is received.

During specific implementation, if the fluid system further comprises a support module and a rotary valve, after the chip module to be cleaned receives the cleaning reagent, the cleaning reagent can be conveyed into the rotary valve through a pipeline and is input into the support module through a port of the rotary valve, and here, the rotary valve can be controlled to input the cleaning reagent into any reagent box of the support module, so that the cleaning of the pipeline between the chip module and the rotary valve and the cleaning of the pipeline between the rotary valve and the support module are completed.

In an optional embodiment, the chip module at least includes a liquid inlet, a liquid outlet and a temperature control module.

The chip module receives a reagent, and performs reagent reaction based on the reagent to generate reagent reaction waste liquid; and when the reagent reaction waste liquid is conveyed to the parallel pump through the valve group, the reagent reaction waste liquid is used for: receiving a reagent from the inlet port; controlling the reaction temperature of the reagent by the temperature control module; and carrying out reagent reaction based on the reagent at the reagent reaction temperature to generate reagent reaction waste liquid, conveying the reagent reaction waste liquid to the valve group from the liquid outlet, and conveying the reagent reaction waste liquid to the gang pump through the valve group.

When the temperature control module is implemented, the chip module comprises a liquid inlet, a liquid outlet and a temperature control module. The temperature control module is used for controlling the temperature in the chip so as to enable the temperature to meet the requirements of reagent reaction. Illustratively, reagent can get into in the chip module through the inlet, carries out reagent reaction under the reagent reaction temperature of control by temperature control module control, produces reagent reaction waste liquid to flow out through the outlet, namely flow into in the valves through the outlet. And the cleaning reagent conveyed by the valve group can flow into the chip module through the liquid outlet and flow into the rotary valve through the liquid inlet.

Referring to fig. 5, the process of the fluid system is illustrated in conjunction with fig. 5. And determining the current chip module in a working state in the plurality of groups of chip modules through the valve position of each electromagnetic valve in the valve group. The current chip module is taken as the chip module 401 for illustration. The pistons of the plurality of syringes 601 of the gang pump are moved from inside to outside (i.e., the pistons are pulled) so that the reagent needles on the rack module 100 draw reagent from the reagent cassette through the reagent holes and input the reagent into the conduit 300, flowing through the conduit 300 into the ports of the rotary valve 200; the reagent enters the pipeline through the common port V of the rotary valve and flows into the chip module 401 through the liquid Inlet Inlet through the pipeline. The chip module 401 performs reagent reaction at the reagent reaction temperature controlled by the temperature control module to obtain reagent reaction waste liquid, and the reagent reaction waste liquid flows out of a pipeline through a liquid Outlet, enters the pipeline, and flows into the valve group 500 through the pipeline, where the connection relationship of each electromagnetic valve in the valve group 500 can be shown in fig. 4; in the valve group 500 in fig. 5, four solenoid valves located in the first row correspond to the solenoid valve 509, the solenoid valve 510, the solenoid valve 511, and the solenoid valve 512 in fig. 4, respectively, and eight solenoid valves located in the second row correspond to the solenoid valves 501 to 508 in fig. 4, respectively. Then flows into the port A of the parallel pump through the valve group 500 and enters the injector of the parallel pump through the port B; reagent reaction waste is transported into waste module 700 through port C and tubing by controlling the piston of the syringe to move from the outside in (i.e., pushing the piston).

When the fluid system is cleaned, the chip module to be cleaned can be determined from the plurality of groups of chip modules through the valve position of each electromagnetic valve in the valve group. The chip module to be cleaned is taken as the chip module 401 for illustration. The pistons of the multiple syringes of the inline pump 600 are moved from the inside to the outside (i.e., the pistons are pulled), so that the cleaning reagent stored in the cleaning module 800 flows into the syringes through the pipes and the B ports of the inline pump. In one mode, by pushing the syringe, the cleaning reagent in the syringe flows into the pipeline through the port C of the tandem pump and is conveyed into the waste liquid module 700 through the pipeline, and the cleaning of the pipeline and the syringe between the syringe and the waste liquid module is completed. In another mode, by pushing the injector, the cleaning reagent in the injector is pushed to the inflow pipeline of the port a of the row pump 600, and is conveyed into the valve group through the pipeline, the cleaning reagent enters the chip module 401 through the liquid Outlet (Outlet) of the chip module 401 by a plurality of electromagnetic valves in the valve group, and then flows into the pipeline through the liquid Inlet (Inlet) of the chip module 401, and is conveyed into the rotary valve 200 through the pipeline; enters the corresponding port of the reagent box through the common port V of the rotary valve and flows into the reagent box of the support module 100 through the corresponding port of the reagent box, so as to complete the cleaning of the chip module and the pipeline.

In the embodiments provided in the present disclosure, it should be understood that the disclosed system may be implemented in other ways. The above-described system embodiments are merely illustrative, and for example, the division of the units is only one type of logical functional division, and other divisions may be realized in practice, and for example, a plurality of units or components may be combined or integrated into another system, or some features may be omitted, or not executed. In addition, the shown or discussed mutual coupling or direct coupling or communication connection may be an indirect coupling or communication connection of devices or units through some communication interfaces, and may be in an electrical, mechanical or other form.

The units described as separate parts may or may not be physically separate, and parts displayed as units may or may not be physical units, may be located in one place, or may be distributed on a plurality of network units. Some or all of the units can be selected according to actual needs to achieve the purpose of the solution of the embodiment.

In addition, functional units in the embodiments of the present disclosure may be integrated into one processing unit, or each unit may exist alone physically, or two or more units are integrated into one unit.

The above are only specific embodiments of the present disclosure, but the scope of the present disclosure is not limited thereto, and any person skilled in the art can easily think of the changes or substitutions within the technical scope of the present disclosure, and shall cover the scope of the present disclosure. Therefore, the protection scope of the present disclosure shall be subject to the protection scope of the claims.

Claims

1. A fluidic system of a gene sequencer, comprising: the device comprises a valve bank, a row pump, a waste liquid module, a pipeline and a plurality of groups of chip modules; each group of chip modules is respectively connected with the valve group through the pipeline, and the parallel pump is respectively connected with the valve group and the waste liquid module through the pipeline;

the current chip module is used for receiving a reagent, and carrying out reagent reaction based on the reagent to generate reagent reaction waste liquid; and the reagent reaction waste liquid is conveyed to the parallel pump through the valve group;

2. The fluid system of claim 1, further comprising: a support module and a rotary valve; the support module is provided with a plurality of reagent kits and reagent needles corresponding to the reagent kits; each reagent needle is respectively connected with one port of the rotary valve; the rotary valve is connected with the plurality of groups of chip modules;

3. The fluid system of claim 1, wherein when each set of the chip modules is connected to the valve block by a plurality of the conduits, the valve block is connected to the inline pump by a plurality of the conduits; the inline pump includes a plurality of injectors; the number of pipelines between the chip module and the valve group is consistent with the number of pipelines between the valve group and the gang pump, and the number of pipelines between the valve group and the gang pump is consistent with the number of injectors;

4. The fluid system of claim 3, further comprising: a cleaning module; the cleaning module is connected with the row pump through a pipeline;

5. The fluid system of claim 4, wherein the valve set is further configured to determine a chip module to be cleaned in the plurality of sets of chip modules based on a valve position of each solenoid valve;

6. The fluidic system of claim 5, wherein when the system comprises a holder module and a rotary valve, the chip module to be washed, upon receiving the washing reagent, is further configured to deliver the washing reagent into a reagent cartridge of the holder module through the rotary valve.

7. The fluid system of claim 1, wherein the chip module comprises at least a liquid inlet, a liquid outlet and a temperature control module;

the chip module receives a reagent, and performs reagent reaction based on the reagent to generate reagent reaction waste liquid; and when the reagent reaction waste liquid is conveyed to the parallel pump through the valve group, the reagent reaction waste liquid is used for:

8. The fluid system of claim 1, wherein the solenoid valve is a two-position, three-way solenoid valve; each electromagnetic valve comprises a first communication port, a second communication port and a third communication port; the electromagnetic valve comprises a first valve position for indicating power on and a second valve position for indicating power off;