CN113750019A

CN113750019A - Panax notoginseng paste mask and preparation method thereof

Info

Publication number: CN113750019A
Application number: CN202111024119.9A
Authority: CN
Inventors: 邱显荣; 董云; 刘盼玉; 曲召辉; 张单单
Original assignee: Zhiran Tiancheng Beijing Biotechnology Co ltd
Current assignee: Zhiran Tiancheng Beijing Biotechnology Co ltd
Priority date: 2021-09-02
Filing date: 2021-09-02
Publication date: 2021-12-07
Anticipated expiration: 2041-09-02
Also published as: CN113750019B

Abstract

The invention discloses a panax notoginseng paste mask and a preparation method thereof, and belongs to the technical field of cosmetics. The panax notoginseng paste-shaped mask comprises panax notoginseng powder, dendrobium stem extract, aloe barbadensis leaf extract, sophora flavescens root extract, fructus lycii ninghamii extract, echinacea purpurea extract, opuntia ficus-indica stem extract, angelica sinensis root extract, panax notoginseng root extract, astragalus membranaceus root extract, ligustrum lucidum ait, licorice root extract and conventional auxiliary materials in the field of cosmetics. The invention also discloses a preparation method of the panax notoginseng paste mask. The panax notoginseng paste mask disclosed by the invention is good in spreadability when being used, easy to wash, good in skin feel after being used, free of tight feeling, delicate in skin, and capable of replenishing water, moisturizing, homogenizing skin color, immediately brightening skin, resisting aging and the like.

Description

Panax notoginseng paste mask and preparation method thereof

Technical Field

The invention relates to the technical field of cosmetics, and particularly relates to a panax notoginseng paste-shaped mask and a preparation method thereof.

Background

In today's society, many professional women typically work around 10 hours a day in front of a computer, causing many skin problems, such as: dark and dry skin color, easy generation of fine lines and the like, and high working pressure, no time and energy for skin maintenance, and accelerated aging of the skin. The facial mask is a beauty and maintenance product commonly used by women with various skin types since ancient times, can not provide nutrition provided by skin basic skin care products, and can also stimulate the maximum vitality of skin in a short time, so that the problem of facial skin is effectively improved. Therefore, consumers have an increasing demand for masks having anti-aging effects.

For the mask, the mud mask contains rich mineral substances and nutrient components, can strongly adsorb dirt in the skin, helps the skin to remove the dirt, and further helps the nutrition of the subsequent skin care products to be effectively absorbed. In recent years, a mud mask has also started to be popular following the face mask. At present, the commercial mud-shaped facial mask mainly has the effects of strongly cleaning dirt, adsorbing blackheads, resisting haze, moistening and nourishing skin, whitening and brightening and the like, and cannot meet the pursuit of consumers for resisting aging. Meanwhile, most of the traditional functional mud masks are added with plant extracts with efficacy on the basis of common paste masks to achieve the effects of moisturizing, oil control and the like, or kaolin or talcum powder is added into common pastes to be prepared into the mud masks by color mixing to absorb skin grease, and the mud masks are rubbed to remove dirt to achieve the effects of oil control, cleaning and the like. However, such a mud film has the phenomena of little effect, poor use feeling and the like.

Disclosure of Invention

In order to solve the problems, the invention provides the panax notoginseng paste mask which is good in smearing property and easy to wash off when in use, has good skin feeling after use, does not have tight feeling, is delicate in skin, and has the effects of moisturizing, homogenizing skin color, brightening immediately and resisting aging.

Therefore, the technical scheme provided by the invention is as follows:

according to the invention, the panax notoginseng paste mask comprises the following components in percentage by mass: 3.0 to 15.0 weight percent of pseudo-ginseng powder, water, butanediol, angelica root extract, pseudo-ginseng root extract, astragalus membranaceus root extract, glossy privet fruit extract, licorice root extract and polyurethane-3510.0 to 15.0 weight percent.

According to the invention, the addition amount of the pseudo-ginseng powder in the pseudo-ginseng paste mask can be 3.0 wt%, 4.0 wt%, 5.0 wt%, 6.0 wt%, 8.0 wt%, 10.0 wt%, 12.0 wt%, 15.0 wt% based on the mass of the pseudo-ginseng paste mask, and points between the above values.

According to the invention, the addition amount of water/butanediol/angelica root extract/pseudo-ginseng root extract/astragalus membranaceus root extract/ligustrum lucidum ait/licorice root extract in the pseudo-ginseng mud mask can be 0.5 wt%, 0.6 wt%, 0.8 wt%, 1.0 wt%, 2.0 wt%, 3.0 wt%, 4.0 wt%, 5.0 wt% based on the mass of the pseudo-ginseng mud mask, and the point values between the above values.

According to the invention, the addition amount of the polyurethane-35 in the panax notoginseng mud mask can be 10.0 wt%, 11.0 wt%, 12.0 wt%, 13.0 wt%, 14.0 wt%, 15.0 wt% based on the mass of the panax notoginseng mud mask, and the points between the above values.

According to the invention, the panax notoginseng paste mask also comprises a humectant, a chelating agent, a thickening agent, an emulsifier, an emollient, a film forming agent, a skin conditioner, a preservative and an essence.

According to the invention, the humectant comprises one or more of glycerol, butanediol, propylene glycol, dipropylene glycol, pentanediol and sodium hyaluronate.

According to the invention, the chelating agent comprises one or more of disodium EDTA, trisodium EDTA, tetrasodium EDTA, and sodium glucoheptonate.

According to the invention, the thickening agent comprises one or more of acryloyl dimethyl ammonium taurate/VP copolymer, xanthan gum, carrageenan, hydroxyethyl cellulose, sodium polyacrylate grafted starch, polyvinyl alcohol, carbomer and acrylic acid (ester)/C10-30 alkanol acrylate cross-linked polymer.

According to the invention, the emulsifier comprises one or more of sucrose stearate, cetearyl glucoside, arachidol glucoside, coco glucoside, glyceryl stearate, PEG-100 stearate, ceteareth-20, methylglucamine sesquistearate.

According to the invention, the emollient comprises one or more of caprylic/capric triglyceride, shea butter, jojoba ester, isostearyl isostearate, isopropyl isostearate, and polydimethylsiloxane.

According to the invention, the film forming agent comprises one or more of polyurethane-35, gum arabic and polyvinylpyrrolidone.

According to the invention, the skin conditioning agent comprises one or more of betaine, tocopherol acetate, panthenol, and glyceryl polyether-26.

According to the invention, the granularity of the pseudo-ginseng powder is 10-60 mu m.

According to some embodiments of the present invention, the particle size of the notoginseng powder is 20 to 50 μm.

According to the invention, the panax notoginseng paste mask comprises the following components in percentage by mass:

phase A: 100 percent of water To, 0.3 To 1.0 percent of acryloyl dimethyl ammonium taurate/VP copolymer, 0.5 To 1.2 percent of polyvinyl alcohol, 0.05 To 0.12 percent of EDTA disodium, 0.01 To 0.05 percent of sodium hyaluronate, 0.05 To 0.12 percent of xanthan gum, 0.3 To 0.8 percent of betaine and 0.78 To 3.0 percent of glycerol polyether-261.0;

phase B: 3.0 to 6.0 weight percent of glycerin, 3.0 to 15.0 weight percent of pseudo-ginseng powder and 2.0 to 6.0 weight percent of butanediol;

and C phase: cetearyl alcohol 2.0 wt% -4.0 wt%, caprylic/capric triglyceride 2.0 wt% -7.0 wt%, isostearyl isostearate 1.0 wt% -3.0 wt%, sucrose stearate/cetearyl glucoside/cetyl alcohol 0.5 wt% -1.5 wt%;

phase D: polyurethane-358.0 wt% -15.0 wt%, water/glycerin/dendrobium stem extract/aloe barbadensis leaf extract/sophora flavescens root extract/1, 2-pentanediol/lycium barbarum fruit extract/1, 2-hexanediol/echinacea purpurea extract 3.0 wt% -8.0 wt%, water/glycerin/trehalose/opuntia ficus indica stem extract/tapioca starch/phenoxyethanol/1, 2-hexanediol 0.5 wt% -2.0 wt%, water/butanediol/angelica root extract/panax notoginseng root extract/astragalus membranaceus root extract/ligustrum lucidum fruit extract/licorice root extract 0.5 wt% -5.0 wt%, caprylyl hydroxamic acid/glyceryl caprylate/1, 2-pentanediol 0.5 wt% -1.0 wt%, 0.1-0.4 wt% of 1, 2-hexanediol and 0-0.04 wt% of essence.

According to some embodiments of the present invention, the panax notoginseng paste mask comprises, by mass:

phase A: 100 percent of water To, 0.3 To 0.8 percent of acryloyl dimethyl ammonium taurate/VP copolymer, 0.8 To 1.2 percent of polyvinyl alcohol, 0.05 To 0.12 percent of EDTA disodium, 0.01 To 0.05 percent of sodium hyaluronate, 0.08 To 0.12 percent of xanthan gum, 0.3 To 0.8 percent of betaine and 0.78 To 3.0 percent of glycerol polyether-261.0;

phase B: 3.0 to 6.0 weight percent of glycerin, 3.0 to 10.0 weight percent of pseudo-ginseng powder and 2.0 to 6.0 weight percent of butanediol;

phase D: polyurethane-3510.0 wt% -15.0 wt%, water/glycerin/dendrobium stem extract/aloe barbadensis leaf extract/sophora flavescens root extract/1, 2-pentanediol/lycium barbarum fruit extract/1, 2-hexanediol/echinacea purpurea extract 3.0 wt% -8.0 wt%, water/glycerin/trehalose/opuntia ficus indica stem extract/tapioca starch/phenoxyethanol/1, 2-hexanediol 0.5 wt% -2.0 wt%, water/butanediol/angelica root extract/panax notoginseng root extract/astragalus membranaceus root extract/ligustrum lucidum fruit extract/licorice root extract 1.5 wt% -5.0 wt%, caprylyl hydroxamic acid/glyceryl caprylate/1, 2-pentanediol 0.5 wt% -1.0 wt%, 0.1-0.4 wt% of 1, 2-hexanediol and 0-0.04 wt% of essence.

phase B: 3.0 to 6.0 weight percent of glycerin, 3.0 to 8.0 weight percent of pseudo-ginseng powder and 2.0 to 6.0 weight percent of butanediol;

phase D: polyurethane-3512.0 wt% -15.0 wt%, water/glycerin/dendrobium stem extract/aloe barbadensis leaf extract/sophora flavescens root extract/1, 2-pentanediol/lycium barbarum fruit extract/1, 2-hexanediol/echinacea purpurea extract 3.0 wt% -8.0 wt%, water/glycerin/trehalose/opuntia ficus indica stem extract/tapioca starch/phenoxyethanol/1, 2-hexanediol 0.5 wt% -2.0 wt%, water/butanediol/angelica root extract/panax notoginseng root extract/astragalus membranaceus root extract/ligustrum lucidum fruit extract/licorice root extract 2.5 wt% -5.0 wt%, caprylyl hydroxamic acid/glyceryl caprylate/1, 2-pentanediol 0.5 wt% -1.0 wt%, 0.1-0.4 wt% of 1, 2-hexanediol and 0-0.04 wt% of essence.

According to still other specific embodiments of the present invention, the panax notoginseng paste mask comprises, by mass:

phase A: 100 percent of water To, 0.3 To 0.5 percent of acryloyl dimethyl ammonium taurate/VP copolymer, 0.8 To 1.0 percent of polyvinyl alcohol, 0.05 To 0.12 percent of EDTA disodium, 0.01 To 0.05 percent of sodium hyaluronate, 0.08 To 0.10 percent of xanthan gum, 0.3 To 0.8 percent of betaine and 0.78 To 3.0 percent of glycerol polyether-261.0;

phase B: 3.0 to 6.0 weight percent of glycerin, 3.0 to 6.0 weight percent of pseudo-ginseng powder and 2.0 to 6.0 weight percent of butanediol;

phase D: polyurethane-3512.0 wt% -15.0 wt%, water/glycerin/dendrobium stem extract/aloe barbadensis leaf extract/sophora flavescens root extract/1, 2-pentanediol/lycium barbarum fruit extract/1, 2-hexanediol/echinacea purpurea extract 3.0 wt% -8.0 wt%, water/glycerin/trehalose/opuntia ficus indica stem extract/tapioca starch/phenoxyethanol/1, 2-hexanediol 0.5 wt% -2.0 wt%, water/butanediol/angelica root extract/panax notoginseng root extract/astragalus membranaceus root extract/ligustrum lucidum fruit extract/licorice root extract 2.5 wt% -4.0 wt%, caprylyl hydroxamic acid/glyceryl caprylate/1, 2-pentanediol 0.5 wt% -1.0 wt%, 0.1 to 0.4 weight percent of 1, 2-hexanediol and 0.01 to 0.04 weight percent of essence.

According to the invention, the hyaluronic acid has a molecular weight of 1.0X 10⁶～1.8×10⁶Da。

According to the present invention, the panax notoginseng paste mask can be prepared by a conventional method in the art.

According to some embodiments of the present invention, the pseudo-ginseng mask is prepared by a preparation method comprising the steps of:

1) respectively dispersing phase A and phase B uniformly, adding phase B into phase A, heating to 80-85 deg.C, and mixing;

2) weighing phase C, heating to 80-85 deg.C, and mixing;

3) adding phase C into the mixed phase of phase A and phase B, homogenizing for 3-5 min;

4) cooling to 45 deg.C, adding phase D, and stirring;

5) cooling to below 38 ℃, filtering and discharging after passing inspection.

In a second aspect, the invention provides a preparation method of the panax notoginseng paste mask of the first aspect.

According to the invention, the preparation method comprises the following steps:

2) weighing phase C, heating to 80-85 deg.C, and mixing;

4) cooling to 45 deg.C, adding phase D, and stirring;

5) cooling to below 38 ℃, filtering and discharging after passing inspection.

The invention has the beneficial effects that:

the multi-effect pasty facial mask is formed by compounding the pseudo-ginseng powder, the angelica root extract, the pseudo-ginseng root extract, the astragalus membranaceus root extract, the glossy privet fruit extract, the licorice root extract and the like according to a specific proportion, and the various effect components are complementary, synergistic and matched, so that skin microcirculation can be better promoted, and the anti-aging effect is achieved.

The mud mask disclosed by the invention is safe and non-irritant, has good spreadability in use, is easy to wash off, has good skin feel after use, no tight feeling and delicate skin, and has the effects of moisturizing, homogenizing skin color, immediately brightening the skin and the like.

Drawings

FIG. 1 is a graph of the relative cell viability of the panax notoginseng paste mask of example 1;

FIG. 2 is the result of the cytomorphological observation of the panax notoginseng paste mask of example 1;

FIG. 3 is the effect of example 1 on the results of COL-I expression assays.

Detailed Description

The invention is further illustrated below with reference to specific examples, to which, however, the invention is not restricted.

It should be appreciated by those skilled in the art that the present invention is not limited to the embodiments, and any changes and modifications to the present invention are within the scope of the present invention.

The experimental methods described in the following examples are all conventional methods unless otherwise specified; the experimental materials and reagents are commercially available, unless otherwise specified.

The raw materials of the invention can be purchased commercially, and the main raw materials and sources used in the invention are shown in table 1:

TABLE 1 raw materials and sources

Examples 1 to 3

Examples 1-3 the components and proportions of the pseudo-ginseng paste mask are shown in table 2.

Table 2 examples 1-3 notoginseng mud mask compositions and proportions

Example 1 a method for preparing a pseudo-ginseng paste mask comprises the following steps:

1) respectively dispersing phase A and phase B uniformly, adding phase B into phase A, heating to 85 deg.C, and mixing uniformly;

2) weighing the phase C, heating to 85 ℃, and uniformly mixing;

3) adding phase C into the mixed phase of phase A and phase B, and homogenizing for 5 min;

4) cooling to 45 deg.C under stirring, adding phase D, and stirring;

5) cooling to 38 ℃, filtering and discharging after passing the inspection.

Example 2 a method for preparing a pseudo-ginseng paste mask comprises the following steps:

1) respectively dispersing phase A and phase B uniformly, adding phase B into phase A, heating to 80 deg.C, and mixing uniformly;

2) weighing the phase C, heating to 80 ℃, and uniformly mixing;

3) adding phase C into the mixed phase of phase A and phase B, and homogenizing for 3 min;

4) cooling to below 40 deg.C under stirring, adding phase D, and stirring;

5) cooling to 35 ℃, filtering and discharging after passing inspection.

Example 3 a method for preparing a pseudo-ginseng paste mask comprises the following steps:

1) respectively dispersing phase A and phase B uniformly, adding phase B into phase A, heating to 83 deg.C, and mixing uniformly;

2) weighing the phase C, heating to 83 ℃, and uniformly mixing;

3) adding phase C into the mixed phase of phase A and phase B, and homogenizing for 4 min;

4) cooling to 45 deg.C under stirring, adding phase D, and stirring;

5) cooling to 35 ℃, filtering and discharging after passing inspection.

Comparative examples 1 to 7

Comparative examples 1-7 ingredients and proportions of the pseudo-ginseng paste mask are shown in table 3.

TABLE 3 COMPARATIVE EXAMPLES 1-7 SANQI MUD-LIKE MASK COMPONENTS AND MIXTURE

Comparative examples 1-7 preparation of a panax notoginseng paste mask the same procedure as in example 1 was used.

Test example 1: skin moisturization test

The skin moisture content is measured based on the capacitance test principle, and at normal temperature, the dielectric constant of water is 81, the dielectric constant of other substances in the skin is usually less than 7, and the water is the substance with the largest dielectric constant in the skin. When the moisture content changes, the capacitance of the skin also changes, so the moisture content on the skin surface can be analyzed by measuring the capacitance of the skin. The greater the skin moisture content value, the higher the skin moisture content, and the better the moisturizing effect.

The human body test of the moisture content of the skin comprises the following specific information:

volunteers: 80 persons (18-45 years old);

test sites: the forearm is bent, and the area of the test area is 3 multiplied by 4cm²The interval between the areas is 1.5 cm.

Test samples: examples 1 to 3, comparative examples 1 to 7.

The use method comprises the following steps: test area the corresponding test sample was smeared, 6 areas were tested per person, each test area (3X 4 cm)²) The sample application amount is 0.5mL, the sample is kept for 10min after being evenly applied, then the test sample is cleaned by clear water, and the residual water is lightly wiped by using a paper towel.

And (3) testing environment: temperature: 20.0-21.5 ℃, relative humidity: 47-49%.

Testing an instrument: skin moisture content tester Corneometer (CM 825, Courage and khazaka, germany).

The test method comprises the following steps: the moisture content of the skin before and after use (0h, 0.5h, 1h, 2h and 4h) is measured by adopting a skin tester, and the moisturizing effect of the facial mask is judged according to the change of the moisture value. The results and the mean change rates are shown in Table 4.

TABLE 4 moisture content of skin before, after 0h, 0.5h, 1h, 2h, 4h of the radix Notoginseng paste facial mask

Note: the above average value is an average of 8 persons in moisture content.

The test results in the table 4 show that compared with the comparative examples 1 to 7, the panax notoginseng paste mask disclosed by the embodiments 1 to 3 can obviously improve the moisture content of the skin, and the moisture content of the skin is obviously improved after the panax notoginseng paste mask is used for 0.5 to 2 hours; after the mask is used for 4 hours, the skin can still keep higher moisture content, which shows that the panax notoginseng paste mask has the moisturizing effect on the basis of supplementing the moisture content of the skin. The pseudo-ginseng paste mask disclosed by the invention utilizes natural plant powder pseudo-ginseng powder and plant extracts to carry formula matrixes, so that the mask has better moisturizing effects.

Test example 2: percutaneous moisture loss test

The skin moisture loss tester Aqua Flux (AF200, BIOX, uk) was tested by collecting water vapor that has diffused through the skin in a closed condensation chamber. The water vapor diffused and frozen on the condensing plate at-7.65 ℃. The air humidity of the part close to the skin in the test cavity is high, the air humidity of the part close to the condenser plate is low, the humidity difference between the test cavity and the condenser plate enables water vapor to move from the surface of the skin to the condenser in a passive diffusion mode, and the water vapor flow density of the test area can be calculated by measuring the humidity gradient. In the instrument, a single silicon chip is used as a sensor for the temperature and humidity of water vapor, and the flow density of the water vapor is measured, so that the water dispersion of the skin is measured. The percutaneous water loss test has the following specific information:

volunteers: 80 persons (18-45 years old);

Test samples: examples 1 to 3, comparative examples 1 to 7.

Testing an instrument: skin moisture loss tester Aqua Flux (AF200, BIOX, uk).

The test method comprises the following steps: the skin moisture loss amount before and after use (0h, 0.5h, 1h, 2h and 4h) is measured by adopting a skin moisture loss tester, and the higher the value of the percutaneous moisture loss TEWL is, the faster the percutaneous moisture loss is. The results and the mean change rates are shown in Table 5.

TABLE 5 Water Dispersion loss of skin before, after 0h, 0.5h, 1h, 2h, 4h of the Panax notoginseng paste mask

Note: the above average value is an average of 8 persons in moisture content.

As shown in table 5, compared with comparative examples 1 to 7, at the same testing time point, the panax notoginseng paste mask of examples 1 to 3 of the present invention has an effect of significantly reducing skin moisture loss, which indicates that the capability of reducing skin moisture loss TEWL of the mask is greatly improved by carrying the formula substrate with the natural plant powder panax notoginseng powder and the plant extract, and is beneficial to fully moisturizing the skin.

Test example 3: sensory evaluation

Volunteers: 100 volunteers 18-50 years old are divided into 10 groups, and no anaphylactic reaction occurs in each group of 30 people;

the using part: the face.

Test samples: example 1, comparative examples 1 to 4.

The use and evaluation methods were: applying 10g of facial mask on face for 15-20min, performing sensory evaluation on the facial mask, evaluating the result by 5 points, investigating the indexes of appearance acceptance, picking up property, smearing uniformity, washing property and the like of the panax notoginseng paste facial mask, scoring between 0 and 5 points according to the using effect, wherein 4 to 5 points are good, 3 to 4 points are good, 2 to 3 points are general, 1 to 2 points are poor, and 0 to1 point is poor, and taking the average value.

TABLE 6 sensory evaluation and evaluation results of the Notoginseng radix paste facial mask

Scoring item	Example 1	Comparative example 1	Comparative example 2	Comparative example 3	Comparative example 4
						Acceptance of appearance	4.35	3.97	4.15	3.38	4.18
Lifting property	4.59	4.12	4.45	4.08	4.39
						Spreadability	4.75	4.00	4.35	4.12	4.15
Uniformity of smearing	4.22	3.73	3.88	3.54	4.07
						Property of washing off	3.93	3.72	3.69	3.18	3.64

Note: the above average values are the average values of 20 human sensory evaluations.

The sensory evaluation results of the panax notoginseng paste mask are shown in table 6, and compared with a comparative example, the panax notoginseng paste mask has the advantages of high appearance acceptance, good lifting, easy smearing, good smearing uniformity and easy washing; meanwhile, volunteers show that the panax notoginseng paste mask has no irritation and has the effects of immediately brightening, moisturizing, tendering skin and the like.

Test example 4: verification of anti-aging efficacy

1. Summary/principles of the experiments

Skin aging is classified into intrinsic aging and extrinsic aging. The former is also called natural aging, which is a natural process of programmed aging. The latter is skin aging due to external environmental factors such as ultraviolet, smoking, blowing, exposure to chemicals. Ultraviolet radiation is the most dominant factor in extrinsic aging. When the skin is irradiated by ultraviolet, excessive active oxygen is generated in cells, so that aging-related genes are expressed, inflammation cascade reaction is induced, the expression quantity of elastin and collagen is reduced, and the skin has aging phenomena such as relaxation, wrinkles and the like.

During skin aging, intracellular MAPK signaling pathways are activated. This pathway inhibits the expression of transforming growth factor beta (TGF- β) while up-regulating the level of Matrix Metalloproteinases (MMP), ultimately leading to a decrease in the amount of type I collagen (COL-I) secretion in the cells, causing skin aging. The amount of COL-I secretion is the most intuitive indicator for skin aging.

The method is based on a human skin fibroblast (HFF-1) model, and the anti-photoaging efficacy of the product is evaluated by measuring the effect of the product on type I collagen after photodamage.

2. Test materials/devices

Super clean bench (Yatai Kelong), inverted microscope (Leica), carbon dioxide incubator (Thermo), electric suction aid (Eppendorf), enzyme labeling instrument (Tecan), ultraviolet irradiation case (Chinese Boda).

3. Test materials

Human skin fibroblasts (HFF-1) were purchased from the stem cell bank of the Chinese academy of sciences.

Fetal bovine serum (FBS, Gibco), DMEM high-sugar medium (DMEM, Gibco), dimethyl sulfoxide (DMSO, Sigma), phosphate buffer (PBS, Gibco), Vitamin E (VE), thiazole blue (MTT, Sigma), human COL-I ELISA kit (Shanghai Weiao).

4. Test method

4.1 HFF-1 cell-based toxicity assays

The test adopts MTT method to detect cell activity and screen the maximum safe concentration of cell sample. The assay was set up with a negative control (medium), a positive control (10% DMSO in medium) and zero-adjusted wells (PBS), the sample concentration settings are shown in table 1, and 3 replicate wells were set for each concentration. The specific operation steps are as follows:

(1) inoculation: digesting logarithmic phase cells, inoculating the cells to a 96-well plate, and placing the plate at 37 ℃ in 5% CO₂And incubating and culturing for 18-24h in the incubator.

(2) Preparing liquid: test substances were prepared at different concentrations according to the concentration settings in table 7.

TABLE 7 sample cytotoxic concentration settings

(3) Sample feeding: removing supernatant after cell growth for 18-24 hr, adding culture medium containing different concentrations of test substance, and placing the culture plate at 37 deg.C and 5% CO₂And incubating and culturing for 18-24h in the incubator.

(4) And (3) detection: after the cells were cultured for 18-24h, the supernatant was discarded, the prepared and filtered MTT (0.5mg/mL) was added, mixed gently and incubated at 37 ℃ for 4h in the dark. Discarding supernatant after incubation, adding 150 μ L DMSO into each well, shaking for 20min, and reading OD with microplate reader_540nmThe value is obtained.

(5) Relative cell viability calculation formula:

4.2 HFF-1 cells COL-I expression detection based on UVA irradiation

(1) Inoculation: cells were seeded into 6-well plates at 37 ℃ with 5% CO₂The incubator is incubated for 18-24 h.

(2) Preparing liquid: the test substances and positive controls were formulated according to table 8.

(3) Sample feeding: the assay was grouped and concentration set according to Table 8, and the cells in the 6-well plates were plated for 18-24h before being platedSamples were given in groups, each group being provided with 3 multiple wells. In group 1, cell culture medium containing 0.1% DMSO was added to both blank and negative controls, and cell culture medium containing 10. mu.g/mL vitamin E was added to the positive control; in group 2, the blank control and the negative control were added with cell culture medium, and the sample group was added with cell culture medium containing samples at corresponding concentrations at 37 ℃ with 5% CO₂The incubator continues to culture for 24 h.

Table 8 test groupings and concentration settings

4) Irradiation: adding sample, culturing for 18-24 hr, removing culture medium, washing with pre-warmed 1mL PBS twice, removing cleaning solution, adding 1mLPBS, and setting the group to be irradiated at 30J/cm²Irradiation was performed at UVA dose. After the irradiation dose was reached, PBS in the wells was removed, washed gently once with 1mL of pre-warmed PBS, and cultured for 18-24h with the addition of DMEM medium.

(5) Detecting the content of the type I collagen: the supernatant was collected and detected by ELISA kit.

(6) Data processing: each item of data obtained in the experiment was processed and plotted by Excel software. Statistical analysis was performed using SPSS 17.0 and comparisons between groups were judged to be significant when P <0.05 using one-way analysis of variance (ANOVA).

5. Test results

5.1 toxicity assay results based on HFF-1 cells

The cytotoxicity test results of the samples are shown in Table 9, the relative cell viability change trend is shown in figure 1, and the morphological observation results of the cells are shown in figure 2.

TABLE 9 sample cytotoxicity test results

Cell efficacy test concentration selection criteria:

(1) relative cell viability ± SD > 85% (no significant difference (P > 0.05) compared to negative control group when mean relative cell viability is between 85-90% (excluding 90%));

(2) cell morphology, no significant change compared to negative control group;

according to the cytotoxicity test result and cell shape, the dosage concentration of the panax notoginseng paste mask on HFF-1 cells is selected to be 0.125%, 0.25% and 0.5%.

5.2 HFF-1 cells COL-I expression detection results based on UVA irradiation

The test was performed according to test method 4.2, and the results are shown in Table 10 and FIG. 3.

TABLE 10 COL-I expression assay results

Note: a tangle-solidup indicates a significant difference compared to SC (UVA-, 0.1% DMSO) group, P < 0.01;

Δ means very significant difference compared to the BC (UVA-) group, P < 0.01;

# indicates that the difference was very significant compared to the SC (UVA +, 0.1% DMSO) group, P < 0.05;

indicates significant difference compared to NC (UVA +) group, P < 0.05.

6. Conclusion

UVA irradiation (UVA +) causes a significant decrease in COL-I expression compared to the UVA non-irradiation (UVA-) group; compared with SC (UVA +, 0.1% DMSO), the PC (VE) group can obviously improve COL-I expression, and the establishment of the test system is shown.

According to the cytotoxicity test result and the cell morphology, the administration concentration of the panax notoginseng paste mask on HFF-1 cells is selected to be 0.125%, 0.25% and 0.5%. The test result shows that compared with NC (UVA +) group, when the concentration is 0.125%, 0.25% and 0.5%, the panax notoginseng mud mask can obviously improve the expression level of type I collagen (COL-I) in cells, which indicates that the panax notoginseng mud mask has the anti-aging effect.

Claims

1. The panax notoginseng paste mask is characterized by comprising the following components in percentage by mass: 3.0 to 15.0 weight percent of pseudo-ginseng powder, water, butanediol, angelica root extract, pseudo-ginseng root extract, astragalus membranaceus root extract, glossy privet fruit extract, licorice root extract and polyurethane-3510.0 to 15.0 weight percent.

2. The panax notoginseng mud mask as claimed in claim 1, further comprising a moisturizing agent, a chelating agent, a thickening agent, an emulsifier, an emollient, a film forming agent, a skin conditioner, a preservative and a perfume.

3. The pseudo-ginseng mud mask as claimed in claim 2, wherein the moisturizer comprises one or more of glycerin, butylene glycol, propylene glycol, dipropylene glycol, pentylene glycol, and sodium hyaluronate, and/or the chelating agent comprises one or more of disodium EDTA, trisodium EDTA, tetrasodium EDTA, and sodium glucoheptonate, and/or the thickener comprises one or more of ammonium acryloyldimethyl taurate/VP copolymer, xanthan gum, carrageenan, hydroxyethyl cellulose, sodium polyacrylate grafted starch, polyvinyl alcohol, carbomer, acrylic acid/C10-30 alkanol acrylate crosspolymer, and/or the emulsifier comprises sucrose stearate, cetearyl glucoside, arachidyl glucoside, cocoyl glucoside, glyceryl stearate, PEG-100 stearate, and mixtures thereof, One or more of ceteareth-20 and methylglucamine sesquistearate, and/or the emollient comprises one or more of caprylic/capric triglyceride, shea butter, jojoba esters, isostearyl isostearate, isopropyl isostearate and polydimethylsiloxane, and/or the film former comprises one or more of polyurethane-35, gum arabic and polyvinylpyrrolidone, and/or the skin conditioner comprises one or more of betaine, tocopherol acetate, panthenol and glyceryl polyether-26.

4. A panax notoginseng paste mask according to any one of claims 1 to 3, wherein the particle size of the panax notoginseng powder is 10 to 60 μm, preferably 20 to 50 μm.

5. The panax notoginseng paste mask is characterized by comprising the following components in percentage by mass:

6. The panax notoginseng paste mask is characterized by comprising the following components in percentage by mass:

7. The panax notoginseng paste mask is characterized by comprising the following components in percentage by mass:

8. The panax notoginseng paste mask is characterized by comprising the following components in percentage by mass:

9. The method for preparing a notoginseng paste mask according to any one of claims 5 to 8, characterized in that the preparation method comprises the steps of:

2) weighing phase C, heating to 80-85 deg.C, and mixing;

4) cooling to 45 deg.C, adding phase D, and stirring;

5) cooling to below 38 ℃, filtering and discharging after passing inspection.