CN113736653A - Liquid adding device for culturing biological tissue - Google Patents

Abstract

The invention discloses a liquid adding device for culturing biological tissues, and relates to the technical field of biological tissue culture; the automatic liquid storage device comprises an open container, a tray, a liquid suction assembly, a transverse moving assembly and a longitudinal moving assembly, wherein the open container is used for storing liquid bottles, a plurality of culture dish placing grooves are arrayed on the upper end face of the tray, the transverse moving assembly is used for driving the longitudinal moving assembly to transversely move, the longitudinal moving assembly is used for driving the liquid suction assembly to longitudinally move, and the open container and the tray are positioned on a moving path of the liquid suction assembly; the imbibition subassembly includes the peg graft pole, first linear drive mechanism, take off a tub jack catch and second linear drive mechanism, the tip is used for pegging graft the imbibition head under the peg graft pole, the peg graft pole is equipped with the suction passageway that is used for connecting negative pressure source and imbibition head, first linear drive mechanism is used for driving the peg graft pole and reciprocates, second linear drive mechanism is used for driving and takes off a tub jack catch and contradicts peg graft pole portion lateral wall, can add biological tissue culture solution to the culture dish automatically, satisfy the demand of mass culture biological tissue.

Description

Liquid adding device for culturing biological tissue

Technical Field

The invention relates to the technical field of biological tissue culture, in particular to a liquid adding device for culturing biological tissue.

Background

In Vitro Fertilization (ivvitro Fertilization) refers to a technique In which sperm and eggs of a mammal complete the Fertilization process In an environment controlled manually In Vitro, abbreviated as IVF. The existing in vitro culture of human embryos is realized by placing a culture dish filled with embryos in a time difference incubator or a conventional carbon dioxide incubator for culture and simultaneously ensuring the temperature, humidity and cleanliness of the culture environment.

When a culture dish for culturing embryos is placed in an incubator, a culture solution for culturing embryos and a cover oil are added to the culture dish. Among the prior art, adopt the mode of artifical liquid feeding to drip culture solution and cover oil into the culture dish in proper order usually, the liquid feeding is efficient, is difficult to satisfy the demand of batched embryo culture.

Disclosure of Invention

Aiming at the technical problem that the automatic, intelligent and batch embryo culture requirements are difficult to meet by adopting manual liquid feeding during the existing human embryo culture; the invention provides a liquid adding device for culturing biological tissues, which can automatically add a biological tissue culture solution into a culture dish and meet the requirement of batch culture of the biological tissues.

The invention is realized by the following technical scheme:

a liquid adding device for culturing biological tissues comprises an open container, a tray, a liquid suction assembly, a transverse moving assembly and a longitudinal moving assembly, wherein the open container is used for storing liquid bottles, a plurality of placing grooves are arrayed on the upper end face of the tray and used for accommodating culture dishes according to set directions, the transverse moving assembly is used for driving the longitudinal moving assembly to transversely move, the longitudinal moving assembly is used for driving the liquid suction assembly to longitudinally move, and the open container and the tray are positioned on the moving path of the liquid suction assembly; the liquid suction assembly comprises an insertion rod, a first linear driving mechanism, a pipe separating clamping jaw and a second linear driving mechanism, wherein the lower end of the insertion rod is used for inserting the liquid suction head, the insertion rod is provided with a suction channel used for connecting a negative pressure source and the liquid suction head, the first linear driving mechanism is used for driving the insertion rod to move up and down, and the second linear driving mechanism is used for driving the pipe separating clamping jaw to abut against the side wall of the rod part of the insertion rod.

When the culture liquid sucking device is used, a sucking channel in the insertion rod is connected with a negative pressure source (for example, the sucking channel is connected with negative pressure generating equipment such as a plunger pump, a peristaltic pump and the like), a culture dish is placed on the tray according to a set position, a corresponding liquid sucking cap is sleeved at the lower end part of the insertion rod, the liquid sucking assembly is moved to the position above a corresponding liquid bottle in the open container through the matching of the transverse moving assembly and the longitudinal moving assembly, then the insertion rod is driven to move downwards through the first linear driving mechanism, the lower end of the liquid sucking head extends into the liquid level in the liquid bottle, and the sucking channel is sucked with negative pressure through the negative pressure source, so that culture liquid is sucked. After the liquid suction head sucks a set amount of culture liquid, the first linear driving mechanism retracts, the liquid suction head is moved out of the open container, the liquid suction assembly is moved to the position above a culture cabin of a designated culture dish through the cooperation of the transverse moving assembly and the longitudinal moving assembly, the insertion rod is driven to move downwards through the first linear driving mechanism again, and after the liquid suction head moves to a set height, negative pressure of a suction channel is removed to enable the culture liquid to be dripped into the culture cabin, and culture addition is completed.

When the liquid suction head sleeved on the insertion rod needs to be taken down, the liquid suction assembly is moved to the upper position of a liquid suction head collection station by the manipulator, the insertion rod is driven to move downwards by the first linear driving mechanism, the upper end of the liquid suction head is located at the lower end of the pipe release clamping jaw, the pipe release clamping jaw is driven by the second linear driving mechanism to move outside the insertion rod to be clamped outside the insertion rod, the insertion rod is driven by the first linear driving mechanism to move up and down, the upper end of the liquid suction head is limited by the pipe release clamping jaw to enable the insertion rod to move relative to the liquid suction head, and the liquid suction head is removed from the insertion head. In conclusion, the invention can automatically absorb the biological tissue culture solution and meet the requirement of batch culture of biological tissues.

In an optional embodiment, the liquid sucking assembly further comprises a cover taking assembly, the cover taking assembly and the liquid sucking assembly are arranged on the longitudinally moving assembly moving part in parallel, the cover taking assembly comprises a third linear driving mechanism and a sucking disc, and the third linear driving mechanism is used for driving the sucking disc to move up and down. The dish cover of the culture dish is uncovered before the culture liquid is dripped into the culture dish, and the dish cover of the culture dish is covered after the sealing oil is added. In an optional embodiment, two insertion rods are arranged in parallel, and the two insertion rods are in driving connection with the corresponding first linear driving mechanism so as to meet the requirement of respectively sucking the culture solution and the cover oil.

In an optional embodiment, the device further comprises a semiconductor refrigeration piece and a hollow cooling body, wherein the semiconductor is used for guiding the heat of the open container to the cooling body, and the cooling body is provided with a cooling medium inlet and a cooling medium outlet. Accessible circulating pump sends into cooling medium and circulates in the cooling body, is cooled off the semiconductor cooling in cooling body or the cooling body by cooling medium to give cooling medium with the heat conduction of the uncovered container of semiconductor absorption, thereby refrigerate uncovered container through the semiconductor refrigeration piece, finally keep warm to the culture solution or the front cover oil in the stock solution bottle.

The semiconductor refrigeration mode is adopted, the container is not required to be kept airtight, and the operation of adding liquid can be still carried out when a liquid bottle for storing biological tissue culture liquid or cover oil is preserved at low temperature. Therefore, the liquid storage bottle can be refrigerated while the liquid is normally added, so that the culture solution or the cover oil in the liquid storage bottle is maintained within a set temperature range, and the phenomenon that the normal culture of biological tissues is influenced due to repeated heating of the culture solution is avoided.

In an optional embodiment, the middle part of the open container is divided into a plurality of open placing cavities with the same size through partition plates, and the placing cavities are used for accommodating liquid bottles so as to store a plurality of liquid bottles at the same time, thereby improving the storage capacity of the storage container.

In an optional embodiment, a side wall of the open container is provided with a liquid discharge port, the bottoms of the placing cavities are communicated through a liquid discharge groove, the liquid discharge groove is arranged around the inner side wall of the bottom of the open container, and the liquid discharge groove is communicated with the liquid discharge port, so that condensed water generated by condensation of water in air in a refrigerating process is prevented from being accumulated in the open container, and the condensed water is prevented from overflowing from the open container to affect a normal preparation liquid for biological tissue culture.

In an optional embodiment, still include the tray frame, tray frame up end is provided with first spout and second spout, first spout is followed tray frame length direction is followed and is stretched, the second spout is followed tray frame width direction extends, just first spout with the second spout is crossing, the tray bottom be provided with first spout with the sliding part of second spout adaptation can realize that the tray pushes, releases from the opposite side from one side of tray frame to avoid the former route return of tray after the liquid feeding is accomplished to cause the interpolation to the culture dish and interfere.

In an optional embodiment, the refrigerator further comprises a box body, a first box door and two longitudinal guide rails, wherein the longitudinal guide rails are provided with moving pieces capable of moving along the length direction of the longitudinal guide rails; the two longitudinal guide rails are positioned on two sides of the upper end of the inner cavity of the box body in the width direction, and the first box door is used for sealing one side surface of the box body in the length direction; two sides of the upper end of the first box door are respectively and rotatably connected with corresponding moving parts, and the first box door can move to be parallel to the plane where the two longitudinal guide rails are located; the open container, the tray, the liquid suction assembly, the transverse moving assembly and the longitudinal moving assembly are all arranged in the box body.

When the first box door needs to be closed, the first box door is moved to one side of the box body, which needs to be closed, of the first box door, when the moving member moves to the tail end corresponding to the longitudinal guide rail, the first box door can be rotated downwards due to the fact that the first box door is rotatably connected with the moving member, and therefore the first box door is closed, and the first box door is attached to the box body under the action of gravity; when the first box door needs to be opened, the first box door is rotated towards the outer side of the box body, then the first box door is pushed towards the direction far away from the first box door, and at the moment, the moving member moves to the other end of the longitudinal guide rail, and the plane where the first box door and the two longitudinal guide rails are located is parallel, so that the first box door slides into the upper end of the box body.

Therefore, in the process of closing the first box door, the first box door firstly slides outwards from the top of the box body along the width direction of the box body and then rotates downwards, so that the first box door cannot rotate towards two sides of the length direction of the box body and cannot rotate towards the top of the box body, and the space on two sides and above the box body is not occupied; in the process of opening the first box door, the first box door rotates to the top of the box body, then stretches into the top of the box body along the width direction of the box body, cannot rotate to the two sides of the length direction of the box body, cannot rotate to the top of the box body, does not occupy the space on the two sides and the top of the box body, and can store the first box door in the box body.

When cultivateing biological tissue, all go on the superclean bench, the superclean bench space is limited, and before adding the culture solution for the culture dish, need add biological tissue into the culture dish, need deliver to culture apparatus with the culture dish after the culture solution adds the completion and cultivate, there are biological tissue application of sample station and cultivation station in culture solution application of liquid station both sides promptly, if adopt to run from opposite directions chamber door or rotatory chamber door, will cause the interference to the operation station of box both sides, if adopt the chamber door of upwards or downward upset then need fix the chamber door, the troublesome poeration of opening and closing of chamber door. And the box that this embodiment provided, the chamber door does not occupy the space of box both sides, also need not be fixed to the chamber door, satisfies the demand that biological component culture solution adds in the box to make biological tissue culture solution can add in the box, avoid culture solution and cover oil to be polluted.

In an optional embodiment, a second openable door is further arranged on one side, opposite to the tray, of the box body in the width direction, so that the culture dish can be placed into the box body from one side of the box body in the width direction, and the culture dish can be taken out from one side of the first door, interference in culture dish adding is avoided, and liquid adding operation of the culture dish is facilitated.

In an optional embodiment, the device further comprises a liquid suction head frame, wherein the liquid suction head frame is used for vertically placing the liquid suction head, and the liquid suction head frame is positioned on the moving path of the liquid suction assembly, so that the liquid suction assembly can be automatically plugged with the liquid suction head.

The invention has the following beneficial effects:

1. the transverse moving assembly and the longitudinal moving assembly drive the liquid suction assembly to move in a plane, so that a liquid suction head of the liquid suction assembly moves back and forth from an open container and a culture dish, the insertion rod is driven to move downwards by the first linear driving mechanism, the lower end of the liquid suction head extends below the liquid level of liquid in the liquid bottle, and the negative pressure source pumps negative pressure to the suction channel, so that culture liquid is sucked; the negative pressure of the suction channel is removed to drop the culture liquid into the culture cabin, the culture addition is completed, the insertion rod is opposite to the pipe-separating clamping jaw to remove the liquid suction head sleeved on the insertion rod, and the automatic replacement of the liquid suction head can be realized.

2. The invention refrigerates the cooling body or cools the semiconductor in the cooling body through the cooling medium to transfer the heat of the open container absorbed by the semiconductor to the cooling medium, so that the open container is refrigerated through the semiconductor refrigerating sheet, the liquid storage bottle is refrigerated while normal liquid adding is ensured, the culture solution or the cover oil in the liquid storage bottle is maintained in a set temperature range, and the normal culture of biological tissues is prevented from being influenced by repeated heating of the culture solution.

3. According to the invention, the orthogonal first sliding groove and second sliding groove are arranged on the upper end surface of the tray frame, so that the tray can be pushed in from one side of the tray frame and pushed out from the other side of the tray frame, interference on the addition of a culture dish caused by the return of the original path of the tray after liquid addition is finished is avoided, and the liquid addition efficiency of the culture solution and the cover oil can be improved.

4. In the process that the first box door is closed, the first box door firstly slides outwards from the top of the box body along the width direction of the box body and then rotates downwards, so that the first box door cannot rotate towards two sides of the length direction of the box body and cannot rotate towards the top of the box body, and the space on two sides and above the box body is not occupied; in-process that opens at first chamber door, first chamber door rotates to the box top, then stretches into the box top along the width direction of box, can not be rotatory to box length direction's both sides, also rotatory to the box top, does not occupy the space of box both sides and top, and can accomodate first chamber door in the box, satisfies the demand that biological component culture solution added in the box to make biological tissue culture solution can add in the box, avoid culture solution and cover oil to be contaminated.

Drawings

In order to more clearly illustrate the technical solutions of the embodiments of the present application, the drawings that are required to be used in the embodiments will be briefly described below, it should be understood that the following drawings only illustrate some embodiments of the present application and therefore should not be considered as limiting the scope, and for those skilled in the art, other related drawings can be obtained from the drawings without inventive effort.

FIG. 1 is a schematic perspective view of a liquid adding device according to an embodiment of the present invention;

FIG. 2 is a schematic perspective view of the internal components of a liquid adding device according to an embodiment of the present invention;

FIG. 3 is a schematic perspective view of a storage container according to an embodiment of the present invention;

FIG. 4 is a schematic top view of a storage container according to an embodiment of the present invention;

FIG. 5 is a schematic view of the structure of the plane A-A of FIG. 4;

FIG. 6 is a schematic perspective view of a bottle sleeve according to an embodiment of the present invention;

FIG. 7 is a schematic perspective view of a tray rack according to an embodiment of the present invention;

FIG. 8 is a perspective view of a tray according to an embodiment of the present invention;

FIG. 9 is a schematic bottom view of a tray according to an embodiment of the present invention;

FIG. 10 is a schematic perspective view of a liquid adding manipulator according to an embodiment of the present invention;

FIG. 11 is a perspective view of a component mounted on the longitudinal movement assembly according to an embodiment of the present invention;

FIG. 12 is a schematic side view of a wicking assembly according to an embodiment of the invention;

FIG. 13 is a schematic structural view of a pipe-releasing jaw mechanism according to an embodiment of the present invention;

FIG. 14 is a schematic view of a door mechanism according to an embodiment of the present invention;

FIG. 15 is a side view of a door mechanism according to an embodiment of the present invention;

fig. 16 is an enlarged schematic view of a portion a of fig. 15.

Reference numerals:

100-open container, 101-partition board, 102-placing cavity, 103-liquid outlet, 104-liquid outlet groove, 110-semiconductor refrigerating sheet, 120-cooling body, 121-cooling medium inlet, 122-cooling medium outlet, 130-liquid bottle and 140-bottle sleeve;

200-a tray frame, 201-a first sliding groove, 202-a second sliding groove, 203-a first positioning part, 204-a second positioning part, 205-a supporting rod;

300-tray, 301-placement groove, 302-handle, 303-sliding part, 304-third positioning part, 305-fourth positioning part;

400-a liquid suction assembly, 401-a plug rod, 402-a liquid suction head, 403-a first linear driving mechanism, 404-a pipe-removing claw and 405-a second linear driving mechanism;

500-cover taking component, 501-sucker, 502-third linear driving mechanism;

600-a traversing assembly;

700-a longitudinal movement assembly;

800-box body, 801-first box door, 802-longitudinal guide rail, 803-moving piece, 804-door handle, 805-decorative strip, 806-limiting part, 807-partition plate and 808-second box door;

900-liquid-absorbing head frame.

Detailed Description

In order to make the objects, technical solutions and advantages of the embodiments of the present application clearer, the technical solutions in the embodiments of the present application will be clearly and completely described below with reference to the drawings in the embodiments of the present application, and it is obvious that the described embodiments are some embodiments of the present application, but not all embodiments. The components of the embodiments of the present application, generally described and illustrated in the figures herein, can be arranged and designed in a wide variety of different configurations.

Thus, the following detailed description of the embodiments of the present application, presented in the accompanying drawings, is not intended to limit the scope of the claimed application, but is merely representative of selected embodiments of the application. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present application.

It should be noted that: like reference numbers and letters refer to like items in the following figures, and thus, once an item is defined in one figure, it need not be further defined and explained in subsequent figures.

In the description of the embodiments of the present application, the terms "central," "upper," "lower," "left," "right," "vertical," "longitudinal," "lateral," "horizontal," "inner," "outer," "front," "rear," "top," "bottom," and the like refer to orientations or positional relationships that are conventionally used in the manufacture of the present application, or that are routinely understood by those of ordinary skill in the art, but are merely used to facilitate the description and to simplify the description and do not indicate or imply that the referenced device or element must have a particular orientation, be constructed and operated in a particular orientation, and therefore should not be considered limiting of the present application.

In the description of the present invention, unless otherwise expressly specified or limited, the terms "disposed," "open," "mounted," "connected," and "connected" are to be construed broadly, e.g., as meaning either a fixed connection, a removable connection, or an integral connection; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meanings of the above terms in the present invention can be understood in specific cases to those skilled in the art.

It should be noted that the embodiments and features of the embodiments in the present application may be combined with each other without conflict.

Examples

With reference to fig. 1 and 2, the present embodiment provides a priming device for culturing biological tissue, comprising an open container 100, a tray 300, a pipetting assembly 400, a traverse assembly 600 and a longitudinal movement assembly 700, wherein the open container 100 is used for storing a liquid bottle 130, a plurality of placement grooves 301 are arrayed on the upper end face of the tray 300, the placement grooves 301 are used for accommodating culture dishes according to a set orientation, the traverse assembly 600 is used for driving the longitudinal movement assembly 700 to move transversely, the longitudinal movement assembly 700 is used for driving the pipetting assembly 400 to move longitudinally, and the open container 100 and the tray 300 are positioned on the moving path of the pipetting assembly 400; the liquid suction assembly 400 comprises a plug rod 401, a first linear driving mechanism 403, a pipe separating clamping jaw 404 and a second linear driving mechanism 405, wherein the lower end of the plug rod 401 is used for plugging a liquid suction head 402, the plug rod 401 is provided with a suction channel used for connecting a negative pressure source and the liquid suction head 402, the first linear driving mechanism 403 is used for driving the plug rod 401 to move up and down, and the second linear driving mechanism 405 is used for driving the pipe separating clamping jaw 404 to abut against the side wall of the rod part of the plug rod 401.

With reference to fig. 5, the storage container for holding the liquid bottle 130 includes not only the open container 100, but also the semiconductor cooling plate 110 and the hollow cooling body 120, the open container 100 is used for holding the liquid bottle 130, the semiconductor is used for guiding the heat of the open container 100 to the cooling body 120, and the cooling body 120 is provided with a cooling medium inlet 121 and a cooling medium outlet 122.

It should be understood that the heated end of the semiconductor chilling plate 110 may be directly attached to the lower end or the sidewall of the open container 100, or may be inserted into the open container 100; in the embodiment shown in fig. 5, since the biological tissue culture fluid is refrigerated, in order to avoid pollution and ensure the refrigeration efficiency, the open container 100 is made of a heat conductive metal (such as aluminum, iron, copper, and alloys thereof), and the heated end of the semiconductor refrigeration sheet 110 is attached to the lower end of the open container 100.

Similarly, the cooling end of the semiconductor chilling plate 110 may be directly attached to the upper end or the sidewall of the cooling body 120, or may be inserted into the cooling body 120; in this embodiment with reference to fig. 5, since the biological tissue culture fluid is refrigerated, in order to avoid pollution and ensure the refrigeration efficiency, the material of the cooling body 120 is a heat conductive metal, and the cooled end of the semiconductor cooling plate 110 is attached to the upper end of the cooling body 120. That is to say, the material of the open container 100 and the cooling body 120 is heat conducting metal, and the conductor refrigeration piece is located between the open container 100 and the cooling body 120, so as to avoid the direct contact of the conductor refrigeration piece with the cooling medium, and ensure the service life of the semiconductor refrigeration piece 110 and the contamination of the culture solution.

With reference to fig. 4 and 5, the middle of the open container 100 is divided into a plurality of open placing cavities 102 with the same size by partitions 101, and the placing cavities 102 are used for accommodating liquid bottles 130 so as to store a plurality of liquid bottles 130 at the same time, thereby improving the storage capacity of the storage container.

Preferably, the bottoms of the placing cavities 102 are communicated, a liquid outlet 103 is arranged on one side wall of the open container 100, and the liquid outlet 103 is used for discharging condensed water in the placing cavities 102 to prevent the condensed water generated by the condensation of water in air in the refrigerating process from accumulating in the open container 100, so as to avoid the condensed water from overflowing from the open container 100 to influence the normal preparation liquid for biological tissue culture.

With continued reference to fig. 4 and 5, the bottoms of the placing cavities 102 are communicated with each other through a drainage groove 104, the drainage groove 104 is disposed around the inner sidewall of the bottom of the open container 100, and the drainage groove 104 is communicated with the drainage port 103, so that the condensed water in the placing cavities 102 is collected through the drainage groove 104.

Preferably, one end of the drain tank 104, which is far away from the drain port 103, is higher than the other end, so that the condensed water in the placing cavity 102 can be completely drained.

Preferably, the outer side wall of the open container 100 is covered with a thermal insulation coating to prevent the open container 100 from heat exchanging with the environment, and avoid the waste of the refrigerating capacity of the semiconductor refrigerating sheet 110.

With reference to fig. 6, the storage container further comprises a bottle sleeve 140 adapted to the placement cavity 102, wherein an open cavity for accommodating the liquid bottle 130 is formed in the middle of the bottle sleeve 140, so that the liquid bottles 130 of corresponding specifications can be accommodated by the bottle sleeves 140 of different specifications, and the storage container can store the liquid bottles 130 of various specifications, thereby ensuring the universality of the storage container. Of course, the fluid bottle 130 may also be placed directly into the placement chamber 102.

With reference to fig. 7, in order to facilitate the placement of the tray 300 according to a set position, the present embodiment further provides a tray rack 200, wherein a first sliding groove 201 and a second sliding groove 202 are disposed on an upper end surface of the tray rack 200, the first sliding groove 201 extends along a length direction of the tray rack 200, the second sliding groove 202 extends along a width direction of the tray rack 200, and the first sliding groove 201 intersects with the second sliding groove 202. Handles 302 are arranged on one side of the tray 300 in the length direction and the width direction, and a sliding part 303 matched with the first sliding groove 201 and the second sliding groove 202 is arranged at the bottom of the tray 300.

Specifically, the shape of the placing groove 301 on the tray 300 is adapted to the shape of the bottom of the culture dish, in this embodiment, a positioning plane is disposed on the side wall of the circular placing groove 301 (the placing groove 301 is in a truncated circular shape) to position the culture dish through the positioning plane, so that accurate position information of the culture dish and the culture dish bottom culture compartment can be obtained when the culture dish is filled with liquid. Handles 302 are provided in both the lengthwise direction and the widthwise direction of the pallet 300 so as to push the pallet 300 into the pallet frame 200 in the lengthwise direction thereof and push the pallet frame 200 in the widthwise direction thereof.

Preferably, the sliding part 303 is a roller provided at the bottom of the tray 300 to reduce resistance when the tray 300 moves.

Continuing to combine with fig. 7, one side of the width direction of the upper end of the tray frame 200 is provided with a first positioning portion 203 to position the tray 300 on the tray frame 200, so as to position the culture dish, and facilitate the automatic addition of the biological tissue culture solution.

Correspondingly, one side of the length direction of the upper end of the tray frame 200 is provided with a second positioning part 204, so that the tray 300 can be accurately positioned through the cooperation of the first positioning part 203 and the second positioning part 204, and therefore, the position error caused by the automatic biological tissue culture is avoided.

Of course, the positioning portions may be disposed on each side above the tray frame 200 for positioning, but the number of the positioning portions is too large, which not only increases the structural cost, but also increases the positioning difficulty, and therefore, usually, only a few positioning portions are disposed at intervals on two adjacent sides of the tray frame 200 orthogonal to the upper end. For the first positioning portion 203 and the second positioning portion 204, which are usually made of or made of magnet-adsorbable materials, the corresponding positioning portions made of magnet or magnet-adsorbable materials are disposed at the corresponding positions of the tray 300.

Further, the second sliding groove 202 is located at an end of the first sliding groove 201 facing the first positioning portion 203, so that when the tray 300 is taken out, the tray 300 can be directly slid along the length direction of the second sliding groove 202, and the volume of the box for accommodating the tray frame 200 of the tray 300 is reduced.

Preferably, the first sliding grooves 201 are spaced two times along the width direction of the tray frame 200 to ensure the stability of the tray 300 when being pushed into the tray frame 200.

Preferably, the second sliding grooves 202 are spaced two times along the length direction of the tray frame 200 to ensure the stability of the tray 300 when pushing out the tray frame 200. At this time, the second sliding chute 202 facing the first positioning portion 203 is located at one end of the first sliding chute 201 facing the first positioning portion 203, and the other second sliding chute 202 is located in the middle of the upper end surface of the tray frame 100.

In the tray rack 200 provided with the two first sliding grooves 201 and the two second sliding grooves 202, four sliding portions 303 are arranged in a rectangular array at the bottom of the tray 300, and the center distance of the sliding portions 303 in the length direction of the tray 300 is adapted to the center distance between the two second sliding grooves 202, and the center distance of the sliding portions 303 in the width direction of the tray 300 is adapted to the center distance between the two first sliding grooves 201. Meanwhile, a third positioning portion 304 corresponding to the first positioning portion 203 and a fourth positioning portion 305 corresponding to the second positioning portion 204, which are made of a magnet or a material that can be attracted by a magnet, are provided at positions corresponding to the tray 300.

Preferably, a guide opening is formed at one end of the first sliding groove 201 to facilitate pushing the tray 300 into the tray frame 200.

Preferably, the support rod 205 is used for supporting the tray frame 200, and the length of the support rod 205 is adjustable, so that the height of the tray 300 can be adjusted conveniently on one hand, and the levelness of the tray 300 on the tray frame 200 can be ensured on the other hand.

Specifically, referring to fig. 10, the traverse unit 600 and the traverse unit 700 are linear driving mechanisms, and only need to be capable of driving the moving members to move linearly, and may be screw slider mechanisms, synchronous pulley mechanisms, gear and gear mechanisms, and the like, which are driven by a motor. In this embodiment, since the load of the traverse module 600 is large, a screw-slider mechanism driven by a motor is used as the traverse module 600, and since the longitudinal movement module 700 needs to be moved quickly and positioned accurately, a synchronous pulley mechanism driven by a motor is used as the longitudinal movement module 700.

Referring to fig. 11, the lower end of the insertion rod 401 is shaped like a stepped shaft with a large top and a small bottom so that the pipette tip 402 is inserted into the lower end of the insertion rod 401.

Preferably, the suction channel penetrates through the insertion rod 401, so that the arrangement of a negative pressure pipeline is facilitated, and the pipeline is prevented from interfering with the movement of the insertion rod 401.

Furthermore, two insertion rods 401 are arranged in parallel, and the two insertion rods 401 are in driving connection with the corresponding first linear driving mechanism 403 so as to meet the requirement of respectively sucking the culture solution and the cover oil. Correspondingly, the pipe-removing clamping jaw assemblies are also provided with two groups.

Referring to fig. 11 and 12, the first linear driving mechanism 403 includes a screw slider mechanism to drive the plugging rod 401 to move up and down through the screw slider mechanism, so that on one hand, the position accuracy of the movement of the plugging rod 401 can be ensured, and on the other hand, the plugging rod 401 can be maintained at a set height, a brake assembly is not required, and the structure of the liquid adding device is simplified.

Preferably, the first linear driving mechanism 403 further includes a synchronous pulley mechanism, and the synchronous pulley mechanism is in transmission connection with the lead screw slider mechanism to ensure the response speed of the first linear driving mechanism 403.

Referring to fig. 13, the tube removing claw 404 is provided with a recess matched with the rod part of the insertion rod 401 to ensure that the tube removing claw 404 can limit the liquid suction head 402 from moving upwards along with the insertion rod 401.

Preferably, the second linear driving mechanism 405 is an electromagnet driving mechanism to simplify the structure and reduce the volume of the liquid filling device.

With reference to fig. 10 and 11, the present embodiment further includes a cap removing assembly 500, the cap removing assembly 500 is disposed on the moving component of the vertical moving assembly 700 in parallel with the liquid suction assembly 400, the cap removing assembly 500 includes a third linear driving mechanism 502 and a suction cup 501, and the third linear driving mechanism 502 is used for driving the suction cup 501 to move up and down. The dish cover of the culture dish is uncovered before the culture liquid is dripped into the culture dish, and the dish cover of the culture dish is covered after the sealing oil is added.

Preferably, the third linear driving mechanism 502 is a synchronous pulley mechanism driven by a motor to ensure the up-and-down moving speed of the suction cap assembly, so as to ensure the liquid adding efficiency of the biological tissue culture solution and the seal oil.

In order to avoid the contamination of the culture solution and the cover oil during the liquid feeding process, the present embodiment further includes a box 800, a first box door 801, and two longitudinal rails 802, wherein the longitudinal rails 802 are provided with a moving member 803 capable of moving along the length direction of the longitudinal rails 802; the two longitudinal guide rails 802 are located on two sides of the upper end of the inner cavity of the box body 800 in the width direction, and the first box door 801 is used for sealing one side surface of the box body 800 in the length direction; two sides of the upper end of the first box door 801 are respectively connected with a corresponding moving member 803 in a rotating manner, and the first box door 801 can move to be parallel to the plane of the two longitudinal guide rails 802; the open top container 100, tray 300, pipetting assembly 400, traversing assembly 600, and traversing assembly 700 are all disposed within the housing 800.

Referring to fig. 15, the moving member 803 is a roller engaged with the longitudinal rail 802, so as to reduce the resistance when the first door 801 is closed or opened. Of course, the moving member 803 may also be a slider slidably connected to the longitudinal guide 802 or a sheave engaged with the longitudinal guide 802

Preferably, a door handle 804 is disposed at a lower end of the first door 801 to facilitate opening and closing of the first door 801.

With reference to fig. 15, the outer sides of the upper and lower ends of the first door 801 are respectively provided with the decorative strips 805 made of plastic material, so that on one hand, the aesthetic property of the first door 801 can be ensured, and on the other hand, the upper and lower ends of the first box 800 can be buffered to absorb the impact when the first door 801 is closed or opened.

Specifically, the material of the decorative strip 805 is ABS plastic, and it can be understood that the material of the decorative strip 805 may be plastic with certain flexibility, such as PP plastic, PA plastic, and the like.

With reference to fig. 15 and 16, the embodiment further includes a limiting portion 806, the limiting portion 806 is located outside one end of the longitudinal rail 802, and the limiting portion 806 is used to limit the moving member 803 on the longitudinal rail 802, so as to prevent the moving member 803 from slipping off the longitudinal rail 802. In use, the stops 806 may be rails disposed outside the upper end of the interior chamber of the case 800.

Further, a partition plate 807 is arranged at the top of the upper end of the inner cavity of the box body 800, and a cavity between the partition plate 807 and the top of the box body 800 can be used for accommodating the first box door 801, so as to prevent the attachments on the first box door 801 from falling to the culture dish filling station.

When the first door 801 needs to be closed, the first door 801 is moved to one side of the box body 800, which needs to be closed, of the first door 801, and when the moving member 803 moves to the end corresponding to the longitudinal guide rail 802, the first door 801 can be rotated downwards due to the fact that the first door 801 is rotatably connected with the moving member 803, so that the first door 801 is closed, and the first door 801 is attached to the box body 800 under the action of gravity; when first door 801 needs to be opened, first door 801 is rotated to the outer side of box body 800, then first door 801 is pushed in the direction away from first door 801, at this time, moving member 803 moves to the other end of longitudinal guide 802, and first door 801 is parallel to the plane of two longitudinal guides 802, so that first door 801 slides into the upper end of box body 800.

That is, in the process of closing the first door 801, the first door 801 slides outward from the top of the box 800 along the width direction of the box 800, and then rotates downward, and does not rotate to the two sides of the length direction of the box 800 or rotate to the top of the box 800, and does not occupy the space on the two sides and above the box 800; in the process of opening the first door 801, the first door 801 rotates towards the top of the box 800, and then extends into the top of the box 800 along the width direction of the box 800, so that the first door cannot rotate towards the two sides of the length direction of the box 800 or rotate towards the top of the box 800, does not occupy the space on the two sides and the top of the box 800, and can accommodate the first door 801 in the box 800.

When cultivateing biological tissue, all go on the superclean bench, the superclean bench space is limited, and before adding the culture solution for the culture dish, need add biological tissue into the culture dish, need deliver to culture apparatus with the culture dish after the culture solution adds the completion and cultivate, there are biological tissue application of sample station and cultivation station in culture solution application of liquid station both sides promptly, if adopt to run from opposite directions chamber door or rotatory chamber door, will cause the interference to the operation station of box 800 both sides, if adopt the chamber door of upwards or downward upset then need fix the chamber door, the troublesome poeration of opening and closing of chamber door. And the chamber door structure that this embodiment provided, the chamber door does not occupy the space of box 800 both sides, also need not be fixed to the chamber door, satisfies the demand that biological component culture solution added in box 800 to make biological tissue culture solution can add in box 800, avoid culture solution and cover oil to be contaminated.

Furthermore, one side of the width direction of the box body 800 is also provided with a second box door 808 which can be opened and closed, so that the culture dish can be placed into the box body 800 from one side of the width direction of the box body 800, and the culture dish can be taken out from one side of the first box door 801, interference caused by adding the culture dish is avoided, and the culture dish feeding operation is facilitated. Since the second door 808 closes one side of the box 800 in the width direction, the second door has a short length and occupies a small space for opening and closing, and the existing revolving door is used in this embodiment.

When the device is used, the liquid bottle 130 for storing the biological tissue culture solution or the cover oil is placed in the placing cavity 102 of the open container 100, the cooling substrate (gas or liquid) is sent into the cooling body 120 through the circulating pump and circulates, the cooling body 120 is cooled or the semiconductors in the cooling body 120 are cooled by the cooling medium, so that the heat of the open container 100 absorbed by the semiconductors is conducted to the cooling medium, the open container 100 is cooled through the semiconductor refrigerating sheet 110, and finally the culture solution or the cover oil in the liquid storage bottle 130 is kept warm.

Placing a culture dish containing biological tissues (such as embryonic tissues, cell organisms and the like) in the placing groove 301 of the tray 300, opening the second door 808 of the box body 800, pushing the tray 300 into the tray frame 200 from the side surface of the box body 800, namely inserting the sliding part 303 at the lower end of the tray 300 into the first sliding groove 201, then pushing the tray 300 along the length direction of the first sliding groove 201, and limiting the tray 300 by the matching of the first positioning part 203 and the third positioning part 304 and the matching of the second positioning part 204 and the fourth positioning part 305 so that the tray 300 is placed at a set position.

Meanwhile, the liquid suction head 402 is placed in the liquid suction head rack 900 in a single state, and the suction channel and the suction cup 501 in the insertion rod 401 are respectively connected with a negative pressure source (for example, connected with a negative pressure generating device such as a plunger pump and a peristaltic pump).

When liquid is added, the transverse moving assembly 600 drives the longitudinal moving assembly 700 to transversely move and longitudinally move the liquid suction assembly 400 to the position above the corresponding socket of the liquid suction head 402, and then the first linear driving mechanism 403 drives the lower end of the insertion rod 401 to be inserted into the socket of the liquid suction head 402 so as to sleeve the liquid suction head 402 at the lower end of the insertion rod 401, so that the inner cavity of the liquid suction head 402 is communicated with the suction channel. The transverse moving component 600 drives the longitudinal moving component 700 to transversely move and the longitudinal moving component 700 moves the imbibing component 400 to the upper part of the culture fluid storage and cover oil bottle, then a first linear driving mechanism 403 drives an inserting rod 401 to move downwards, the lower end of an imbibing head 402 on the inserting rod 401 extends into the liquid level in the liquid bottle 130, and then a negative pressure source pumps negative pressure to the imbibing channel, thereby imbibing the culture fluid; another plug rod 401 is driven to move downwards by another first linear driving mechanism 403, the lower end of a liquid suction head 402 on the plug rod 401 extends into the seal oil, and negative pressure is pumped from a negative pressure source to a suction channel, so that the seal oil is sucked.

After the liquid suction heads 402 suck a set amount of culture liquid, the two first linear driving mechanisms 403 retract, the two liquid suction heads 402 are respectively moved out of the storage liquid bottles of the culture liquid and the cover oil, the transverse moving assembly 600 drives the longitudinal moving assembly 700 to transversely move and the longitudinal moving assembly 700 moves the cover taking assembly 500 to the upper side of the culture dish, then the third linear driving mechanism 502 drives the suction disc 501 to downwards move to tightly cling to the dish cover of the culture dish, and then the third linear driving mechanism 502 retracts to upwards move the dish cover.

After the dish is taken down, the transverse moving assembly 600 drives the longitudinal moving assembly 700 to transversely move, the longitudinal moving assembly 700 moves the dish cover to one side above the culture dish, the imbibing assembly 400 is moved to the upper part of a culture cabin of the culture dish, the insertion rod 401 is driven to downwards move by the first linear driving mechanism 403 again, and when the imbibing head 402 at the lower end of the insertion rod 401 moves to a set height, the negative pressure of the imbibing channel is removed to drop the culture liquid in the culture cabin, so that the culture addition is completed; then the traverse motion assembly 600 drives the longitudinal motion assembly 700 to traverse again, the longitudinal motion assembly 700 moves another plug rod 401 to the upper part of the culture dish, another first linear driving mechanism 403 drives the plug rod 401 to move downwards, and when the liquid suction head 402 at the lower end of the plug rod 401 moves to a set height, the negative pressure of the suction channel is removed to drop the cover oil into the culture cabin, so that the addition of the cover oil is completed.

After the addition of the biological tissue culture solution and the cover oil required for all the culture dishes is completed, the first door 801 is opened, and the take-out tray 300 is slid along the length direction of the second chute 202 to feed the culture dishes into the incubator for culture.

When the liquid suction head 402 sleeved on the insertion rod 401 needs to be taken down, the manipulator moves the liquid suction assembly to the position on the liquid suction head 402 collecting station, the insertion rod 401 is driven to move downwards through the first linear driving mechanism 403, the upper end of the liquid suction head 402 is located at the lower end of the tube disengaging jaw 404, the tube disengaging jaw 404 is driven by the second linear driving mechanism to be clamped outside the insertion rod 401 through the outer side movement of the insertion rod 401, then the insertion rod 401 is driven to move up and down through the first linear driving mechanism 403, the upper end of the liquid suction head 402 is limited through the tube disengaging jaw 404, so that the insertion rod 401 and the liquid suction head 402 move relatively, and the liquid suction head 402 is removed from the insertion head.

Wherein, when automatic biological tissue culture liquid that adds, the culture dish all is placed in box 800, and goes on the superclean bench, before tray 300 gets into the box, need place the station at the culture dish is automatic and add the culture dish to tray 300 on, return to the interpolation that will cause the interference to the culture dish if the former route of tray 300 after the liquid feeding is accomplished, tray 300 can only push, release from one side of box length direction from one side of the width direction of box. And this embodiment is provided with orthogonal first spout 201 and second spout 202 on tray frame 200 up end, can realize that tray 300 pushes from one side of tray frame 200, release from the opposite side, satisfies the demand that biological tissue batched was cultivateed to make biological tissue culture solution can add in box 800, avoid culture solution and cover oil to be contaminated.

Moreover, the semiconductor refrigeration mode is adopted, so that the liquid bottle 130 for storing the biological tissue culture solution or the seal oil can still be filled when the liquid bottle is preserved at low temperature without keeping the container closed. Therefore, the present embodiment can refrigerate the liquid storage bottle 130 while normally adding liquid, so that the culture solution or the cover oil in the liquid storage bottle 130 is maintained within the set temperature range, and the culture solution is prevented from being repeatedly heated to affect the normal culture of the biological tissue.

To sum up, this embodiment can absorb biological tissue culture solution, cover oil automatically to and add culture solution and cover oil to the culture dish in, satisfy the demand that biological tissue batched was cultivateed.

The foregoing is only a preferred embodiment of the present invention, and the present invention is not limited thereto in any way, and any simple modification, equivalent replacement and improvement made to the above embodiment within the spirit and principle of the present invention still fall within the protection scope of the present invention.

Claims (10)

1. A priming device for culturing biological tissues, which is characterized by comprising an open container (100), a tray (300), a liquid suction assembly (400), a traverse assembly (600) and a longitudinal moving assembly (700), wherein the open container (100) is used for storing a liquid bottle (130), a plurality of placing grooves (301) are arrayed on the upper end face of the tray (300), the placing grooves (301) are used for accommodating culture dishes according to a set orientation, the traverse assembly (600) is used for driving the longitudinal moving assembly (700) to move transversely, the longitudinal moving assembly (700) is used for driving the liquid suction assembly (400) to move longitudinally, and the open container (100) and the tray (300) are positioned on the moving path of the liquid suction assembly (400);

the liquid suction assembly (400) comprises an insertion rod (401), a first linear driving mechanism (403), a pipe separating clamping jaw (404) and a second linear driving mechanism (405), wherein the lower end of the insertion rod (401) is used for inserting a liquid suction head (402), the insertion rod (401) is provided with a suction channel used for connecting a negative pressure source and the liquid suction head (402), the first linear driving mechanism (403) is used for driving the insertion rod (401) to move up and down, and the second linear driving mechanism (405) is used for driving the pipe separating clamping jaw (404) to abut against the side wall of the rod part of the insertion rod (401).

2. The priming device for culturing biological tissue according to claim 1, further comprising a cap-removing assembly (500), wherein said cap-removing assembly (500) is juxtaposed to said pipetting assembly (400) on said moving part of said longitudinal moving assembly (700), said cap-removing assembly (500) comprises a third linear driving mechanism (502) and a suction cup (501), said third linear driving mechanism (502) is used for driving said suction cup (501) to move up and down.

3. The priming device for the culture of biological tissues according to claim 2, characterized in that two insertion rods (401) are arranged side by side, and the two insertion rods (401) are in driving connection with the corresponding first linear driving mechanism (403).

4. The charging device for culturing biological tissue according to claim 1, further comprising a semiconductor cooling plate (110) and a hollow cooling body (120), said semiconductor being adapted to conduct heat of said open container (100) to said cooling body (120), said cooling body (120) being provided with a cooling medium inlet (121) and a cooling medium outlet (122).

5. The priming device for biological tissue culture according to claim 1, characterized in that said open container (100) is divided in its middle into a plurality of open placement chambers (102) of the same size by partitions (101), said placement chambers (102) being adapted to receive liquid bottles (130).

6. The priming device for culturing biological tissues according to claim 5, wherein a drain port (103) is formed on one side wall of said open container (100), the bottoms of said plurality of placing cavities (102) are communicated through a drain groove (104), said drain groove (104) is formed around the inner side wall of the bottom of said open container (100), and said drain groove (104) is communicated with said drain port (103).

7. The priming device for culturing biological tissues according to claim 1, further comprising a tray frame (200), wherein a first sliding chute (201) and a second sliding chute (202) are arranged on the upper end face of the tray frame (200), the first sliding chute (201) extends along the length direction of the tray frame (200), the second sliding chute (202) extends along the width direction of the tray frame (200), the first sliding chute (201) intersects with the second sliding chute (202), and a sliding portion (303) matched with the first sliding chute (201) and the second sliding chute (202) is arranged at the bottom of the tray (300).

8. The priming device for the culture of biological tissues according to claim 1, further comprising a box (800), a first box door (801) and two longitudinal guides (802), wherein said longitudinal guides (802) are provided with a moving member (803) capable of moving along the length direction of said longitudinal guides (802);

the two longitudinal guide rails (802) are positioned on two sides of the upper end of the inner cavity of the box body (800) in the width direction, and the first box door (801) is used for sealing one side surface of the box body (800) in the length direction;

two sides of the upper end of the first box door (801) are respectively connected with corresponding moving parts (803) in a rotating mode, and the first box door (801) can move to be parallel to the plane where the two longitudinal guide rails (802) are located;

the open container (100), the tray (300), the liquid suction assembly (400), the transverse moving assembly (600) and the longitudinal moving assembly (700) are all arranged in the box body (800).

9. The priming device for culturing biological tissues according to claim 8, wherein a second door (808) which can be opened and closed is further arranged on one side of the box body (800) which faces the tray (300) in the width direction.

10. The priming device for the cultivation of biological tissues according to claim 1, characterized in that it further comprises a pipette head (900), said pipette head (900) being adapted to vertically position the pipette head (402), said pipette head (900) being located on the path of movement of said pipetting assembly (400).

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