CN113729112A - High-activity biological agent for removing pet parasitic toxin and preparation method thereof - Google Patents
High-activity biological agent for removing pet parasitic toxin and preparation method thereof Download PDFInfo
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Abstract
The application relates to the technical field of biological agents, and particularly discloses a high-activity biological agent capable of removing parasite toxins in pets and a preparation method thereof. The high-activity biological preparation capable of removing the parasite toxin in the pet comprises 45-50 parts of composite probiotic bacteria liquid, 25-30 parts of freeze-drying protective carrier and 3-5 parts of polyvinyl alcohol solution, wherein the freeze-drying protective carrier comprises the following substances in parts by weight: 3-5 parts of glucose, 3-5 parts of lactose, 25-30 parts of protein powder and 15-30 parts of water. The preparation method comprises the following steps: s1, preparing a probiotic agent; s2, pre-mixing; and S3, blending. This application has selected compound probiotic fungus liquid to handle the internal parasite toxin of pet and has restoreed the internal environment of pet, prevents the further erosion of parasite toxin to the intestinal mucosa, and through forming stable protection area, and then release bacteriocin simultaneously, reach and restore pet intestinal mucosa, get rid of the effect of pet internal parasite toxin.
Description
Technical Field
The invention belongs to the technical field of biological agents, and particularly relates to a high-activity biological agent for removing pet parasitic toxins and a preparation method thereof.
Background
Parasitic diseases are common diseases of pets, parasites inside and outside a body can deprive pet nutrition to different degrees, tissue cells are damaged, toxins are secreted, growth and development of the pets are influenced, and healthy intestinal parasitic diseases are frequently diseases of the pets, and the parasites mainly comprise roundworms, tapeworms, hookworms, coccidian, trematodes, giardia flagellates and the like.
Among them, pet parasites are mainly classified into ectoparasites and endoparasites, wherein the endoparasites are harmful to the pets, and generally, the endoparasites cause appetite decrease, poor spirit, malnutrition, diarrhea, hematochezia and the like of the pets. Metabolic products and secretions and excretions of the parasite during life, such as certain enzymes, body fluids released by disintegration of the parasite when it dies, and the like are all chemicals harmful to the host, collectively known as toxins. Can trigger local or systemic reactions, and some parasites can also cause host allergic reactions.
Traditional parasite control mainly aims at expelling parasites by using medicaments, although the effect is obvious, because parasites leave a large amount of toxins in pet bodies, long-term damage is easily formed in pet intestinal tracts, and the intestinal functions of pets tend to be healthy by long-time self-system repair.
Disclosure of Invention
In order to overcome the defect that the existing pet body is easy to have residual toxin after expelling parasites and harmful to the health of pets, the invention provides a high-activity biological agent for removing the parasite toxin of pets and a preparation method thereof, and the following technical scheme is adopted:
in a first aspect, the invention provides a high-activity biological agent for removing pet parasite toxin, which adopts the following technical scheme:
a high-activity biological agent for removing parasite toxins of pets comprises the following substances in parts by weight:
45-50 parts of composite probiotic bacteria liquid;
25-30 parts of a freeze-drying protective carrier;
3-5 parts of a polyvinyl alcohol solution;
the freeze-drying protection carrier comprises the following substances in parts by weight:
3-5 parts of glucose;
3-5 parts of lactose;
25-30 parts of protein powder;
15-30 parts of water.
By adopting the technical scheme, the parasite toxin in the pet body is treated by the composite probiotic bacteria liquid and the internal environment of the pet body is repaired, the intestinal mucosa in the pet body is greatly damaged by the parasite toxin, and the composite probiotic bacteria is fixed on the intestinal mucosa by the repairing effect of the composite probiotic bacteria liquid, so that the further erosion of the intestinal mucosa by the parasite toxin is prevented. Secondly, the composite probiotics existing in the intestinal tract can be effectively distributed in different regions of the intestinal tract to form stable protection regions, so that bacteriocin is released, the invasion of competitors is inhibited, and the effects of repairing the intestinal mucosa of the pet and removing the parasite toxin in the pet are achieved.
Meanwhile, the freeze-drying protection carrier used in the application has a good protection effect on the probiotic bacteria liquid during preparation of the microbial preparation, so that the damage of the freezing process and the drying process to the composite probiotics is reduced, the original various physiological and biochemical characteristics and biological activity are kept as far as possible, and the survival rate of the microbial preparation is improved.
Further, the composite probiotic bacteria liquid is prepared by mixing bacillus liquid, lactobacillus liquid and saccharomycete liquid, wherein the mass ratio of the bacillus liquid to the lactobacillus liquid to the saccharomycete liquid is 2: 3: 1.
through adopting above-mentioned technical scheme, the composition of compound probiotic fungus liquid has been optimized in this application, because the bacillus is a kind of facultative aerobe that sporadically exists in the intestinal, has characteristics such as high temperature resistant, acid and alkali-resistance and resistant cholate, can produce nutrients such as vitamin, amino acid, somatomedin and multiple enzymes such as amylase, lipase, protease, promotes the digestion and absorption of nutrients. The lactobacillus can decompose saccharides to generate lactic acid, can form synergistic combination with the bacillus, and generates a large amount of enzymes, hydrogen peroxide, organic acids, bacteriocins and the like by competitively excluding the combination of pathogenic bacteria and intestinal mucosa epithelial cells, thereby inhibiting the growth and propagation of harmful bacteria, promoting the intestinal peristalsis and discharging parasite toxins. Finally, the continuous proliferation of bacillus and lactic acid bacteria is stimulated by saccharomycetes, so that the intestinal health environment is improved, and the immune function of an organism is enhanced.
Further, the high-activity biological preparation for removing the pet parasitic toxins further comprises 45-50 parts of a slow release carrier, wherein the slow release carrier is a porous polypeptide slow release carrier, and the porosity of the porous polypeptide slow release carrier is 45-50%.
By adopting the technical scheme, the polypeptide slow-release material is used as the carrier, and the polypeptide protein has large relative molecular mass, so that the decomposition of active substances in the biological preparation in a strong acid environment and an enzyme environment in the stomach can be effectively prevented after the polypeptide slow-release material is used, the activity of the biological preparation is protected by taking the polypeptide slow-release material as a protective carrier, and the retention time of the composite probiotic biological preparation in the gastrointestinal tract is effectively prolonged.
Meanwhile, the slow release carrier adopted by the application is a porous material, so that the loading capacity of the polypeptide slow release carrier to the active ingredients of the biological preparation can be further improved, and the activity and the repairing effect of the biological preparation can be effectively improved.
Further, the porous polypeptide slow-release carrier is prepared by adopting the following scheme:
(1) stirring and mixing the protein polypeptide and the sodium alginate solution according to the mass ratio of 1: 3-5, collecting the mixed solution and placing the mixed solution in a spray drying device;
(2) spraying and pressing the particles generated by drying in a spray drying device into a calcium chloride solution, stirring and crosslinking after the spraying and pressing are finished, and collecting a crosslinking mixed solution;
(3) and filtering the crosslinking mixed solution, taking a filter cake, washing with deionized water, carrying out vacuum freeze drying, and collecting the porous polypeptide slow-release carrier.
By adopting the technical scheme, the preparation scheme of the porous polypeptide slow-release carrier is optimized, the retention time in the gastrointestinal tract can be prolonged to a certain extent by virtue of a microsphere structure prepared by spray drying, and the calcium alginate microspheres formed by crosslinking sodium alginate and calcium ions have pH responsiveness, namely, the calcium alginate microspheres shrink in an acid environment and swell in an alkaline environment to release the drug, so that the biological preparation can be protected from the influence of the pH environment in the gastrointestinal tract and protease, and the drug release is realized after the biological preparation reaches the intestinal part, and the targeted drug delivery is realized.
Further, the high-activity biological agent also comprises 6-8 parts of repair compound peptide, and the repair compound peptide is prepared by adopting the following scheme:
(1) placing wheat protein in deionized water, stirring and collecting to obtain a suspension;
(2) adjusting the pH of the suspension to 5.5 by using a sodium hydroxide solution or hydrochloric acid, and adding protease for enzymolysis;
(3) collecting the suspension after enzymolysis, heating, boiling, inactivating enzyme, cooling in ice water bath, centrifuging, collecting the supernatant, vacuum freeze drying, and collecting to obtain the repair peptide.
By adopting the technical scheme, the repairing composite peptide formed by enzymolysis of the millet protein can be quickly decomposed into glycyl glutamine in vivo, and the substance is the only energy source of small intestinal mucosa cells and the most important nutrient substance for repairing intestinal tracts, so that the intestinal mucosa can be effectively repaired, the intestinal mucosa is prevented from being withered, the intestinal immunologic function is improved, the weight, the structure and the protein content of the normal intestinal mucosa are maintained, and the parasitic toxin is effectively removed to a certain degree, so that the physique and the function of pets are effectively improved.
Further, the protease comprises mixed enzyme of pepsin and trypsin with equal mass.
By adopting the technical scheme, protease composition components are preferably selected, so that the purity of the repair composite peptide prepared by enzymolysis is better, and the repair composite peptide can be used for repairing and detoxifying the body of a pet more excellently.
Further, the high-activity biological agent also comprises 3-5 parts of plant essential oil microcapsules.
By adopting the technical scheme, the plant essential oil microcapsule is adopted in the pet food, the release rate of the plant essential oil in a pet body can be improved, so that the retention time of the plant essential oil in the pet body is prolonged, and the treatment effect of a high-activity biological agent on harmful bacteria is further improved.
Further, the plant essential oil comprises any one or more of clove essential oil, marigold essential oil, balm essential oil or impatiens balsamina essential oil.
By adopting the technical scheme, the main chemical components in the clove flower essential oil, the marigold essential oil, the balm flower essential oil or the impatiens balsamina essential oil which are optimized in the application are plant polyphenol substances which can have stronger inhibition effect on harmful mixed bacteria such as candida albicans, aspergillus, dermatophytosis and the like, and the inhibition effect of the high-activity biological agent on the harmful mixed bacteria is improved.
In a second aspect, the invention provides a preparation method of a high-activity biological agent for removing pet parasite toxins, which adopts the following technical scheme:
s1, preparation of probiotic bacteria agent: firstly, mixing the composite probiotic bacteria liquid with a freeze-drying protective carrier, stirring, grinding and dispersing, collecting dispersed slurry, then carrying out vacuum freeze drying, and collecting the probiotic bacteria agent;
s2, pre-mixing treatment: stirring and mixing the plant essential oil microcapsule, the repair compound peptide and the probiotic agent, and collecting to obtain a premix;
s3, blending treatment: and stirring and mixing the uniformly mixed material, the polyvinyl alcohol solution and the porous polypeptide slow-release carrier, crushing and sieving after vacuum freeze drying, and collecting the high-activity biological preparation for removing the pet parasitic toxin.
By adopting the technical scheme, the scheme of multiple mixing preparation is adopted to prepare the high-activity biological preparation for removing the pet parasite toxin, the uniform dispersion performance among all components is improved through premixing, the integrity among all premixed substances is further improved, and the service life of the high-activity biological preparation for removing the pet parasite toxin is prolonged.
In summary, the present application includes at least one of the following beneficial technical effects:
first, this application has selected compound probiotic fungus liquid to handle the internal parasite toxin of pet and has restoreed the internal environment of pet, because parasite toxin can carry out the destructiveness greatly to the internal intestinal mucosa of pet, through the restoration effect of compound probiotic fungus liquid, makes compound probiotic definite value on the intestinal mucosa, prevents the further erosion of parasite toxin to the intestinal mucosa. Secondly, the composite probiotics existing in the intestinal tract can be effectively distributed in different regions of the intestinal tract to form stable protection regions, so that bacteriocin is released, the invasion of competitors is inhibited, and the effects of repairing the intestinal mucosa of the pet and removing the parasite toxin in the pet are achieved.
Meanwhile, the freeze-drying protection carrier used in the application has a good protection effect on the probiotic bacteria liquid during preparation of the microbial preparation, so that the damage of the freezing process and the drying process to the composite probiotics is reduced, the original various physiological and biochemical characteristics and biological activity are kept as far as possible, and the survival rate of the microbial preparation is improved.
Secondly, the polypeptide slow-release material is used as a carrier, and due to the fact that the polypeptide protein is large in relative molecular mass, decomposition of active substances in the biological preparation by a strong acid environment and an enzyme environment in the stomach can be effectively prevented after the polypeptide slow-release material is used, and the polypeptide slow-release material is used as a protection carrier, so that the activity of the biological preparation is protected, and the retention time of the composite probiotic biological preparation in the gastrointestinal tract is effectively prolonged.
Meanwhile, the slow release carrier adopted by the application is a porous material, so that the loading capacity of the polypeptide slow release carrier to the active ingredients of the biological preparation can be further improved, and the activity and the repairing effect of the biological preparation can be effectively improved.
Thirdly, the repairing compound peptide formed by the enzymolysis of the millet protein can be quickly decomposed into glycyl glutamine in vivo, and the substance is the only energy source of small intestinal mucosa cells and the most important nutrient substance for repairing intestinal tracts, so that the intestinal mucosa can be effectively repaired, the intestinal mucosa is prevented from being withered, the intestinal immunologic function is improved, the weight, the structure and the protein content of the normal intestinal mucosa are maintained, and the parasite toxin is effectively removed to a certain degree, so that the physique and the function of pets are effectively improved.
And fourthly, the plant essential oil microcapsule is adopted, so that the release rate of the plant essential oil in the pet body can be improved, the retention time of the plant essential oil in the pet body is prolonged, and the treatment effect of the high-activity biological agent on harmful bacteria is further improved.
Meanwhile, the main chemical components in the clove essential oil, the marigold essential oil, the balm essential oil or the impatiens essential oil which are optimized in the application are plant polyphenol substances which can have strong inhibition effect on harmful mixed bacteria such as candida albicans, aspergillus, dermatophytosis and the like, and the inhibition effect of the high-activity biological agent on the harmful mixed bacteria is improved.
Detailed Description
The present application will be described in further detail with reference to examples and comparative examples.
The starting materials in this application may be commercially available products, and the following sources are merely exemplary and are not representative of the indicated starting materials.
Preparation example
Preparation example 1
Preparation of a freeze-drying protective carrier:
stirring and mixing 3 parts of glucose, 3 parts of lactose, 25 parts of protein powder and 15 parts of water, grinding and dispersing, and collecting the freeze-drying protective carrier 1.
Preparation example 2
Preparation of a freeze-drying protective carrier:
stirring and mixing 4 parts of glucose, 4 parts of lactose, 27 parts of albumen powder and 22 parts of water, grinding and dispersing, and collecting the freeze-drying protective carrier 2.
Preparation example 3
Preparation of a freeze-drying protective carrier:
5 parts of glucose, 5 parts of lactose, 30 parts of protein powder and 30 parts of water are stirred, mixed, ground and dispersed, and the freeze-drying protective carrier 3 is obtained after collection.
Preparation example 4
Preparing a porous polypeptide slow-release carrier:
(1) stirring and mixing 1 part of protein polypeptide and 3 parts of sodium alginate solution, collecting the mixed solution and placing the mixed solution in a spray drying device;
(2) spraying and pressing the particles generated by drying in a spray drying device into 20 parts of calcium chloride solution, stirring and crosslinking after the spraying and pressing are finished, and collecting a crosslinking mixed solution;
(3) and filtering the crosslinking mixed solution, taking a filter cake, washing with deionized water, carrying out vacuum freeze drying, and collecting the porous polypeptide sustained-release carrier 1 with the porosity of 45%.
Preparation example 5
Preparing a porous polypeptide slow-release carrier:
(1) stirring and mixing 1 part of protein polypeptide and 4 parts of sodium alginate solution, collecting the mixed solution and placing the mixed solution in a spray drying device;
(2) spraying and pressing the particles generated by drying in a spray drying device into 20 parts of calcium chloride solution, stirring and crosslinking after the spraying and pressing are finished, and collecting a crosslinking mixed solution;
(3) and filtering the crosslinking mixed solution, taking a filter cake, washing with deionized water, carrying out vacuum freeze drying, and collecting the porous polypeptide sustained-release carrier 2 with the porosity of 47%.
Preparation example 6
Preparing a porous polypeptide slow-release carrier:
(1) stirring and mixing 1 part of protein polypeptide and 5 parts of sodium alginate solution, collecting the mixed solution and placing the mixed solution in a spray drying device;
(2) spraying and pressing the particles generated by drying in a spray drying device into 20 parts of calcium chloride solution, stirring and crosslinking after the spraying and pressing are finished, and collecting a crosslinking mixed solution;
(3) and filtering the crosslinking mixed solution, taking a filter cake, washing with deionized water, carrying out vacuum freeze drying, and collecting the porous polypeptide sustained-release carrier 3 with the porosity of 50%.
Preparation example 7
Preparation of repair complex peptide
(1) Taking 0.1 part of wheat protein, placing the wheat protein in 10 parts of deionized water, stirring and collecting to obtain a suspension;
(2) adjusting the pH of the suspension to 5.5 by using 0.1mol/L sodium hydroxide solution or 0.1mol/L hydrochloric acid, adding mixed enzyme prepared by mixing pepsin and trypsin with equal mass, and performing enzymolysis treatment by controlling the addition amount to be 50 u/g;
(3) collecting the suspension after enzymolysis, heating to 110 deg.C, boiling, inactivating enzyme, cooling in ice water bath at 0 deg.C, centrifuging, collecting the supernatant, vacuum freeze drying, and collecting to obtain repair peptide 1.
Preparation example 8
Preparation of repair complex peptide
(1) Taking 0.1 part of wheat protein, placing the wheat protein in 12 parts of deionized water, stirring and collecting to obtain a suspension;
(2) adjusting the pH of the suspension to 5.5 by using 0.1mol/L sodium hydroxide solution or 0.1mol/L hydrochloric acid, adding mixed enzyme prepared by mixing pepsin and trypsin with equal mass, and performing enzymolysis treatment by controlling the addition amount to be 50 u/g;
(3) collecting the suspension after enzymolysis, heating to 110 deg.C, boiling, inactivating enzyme, cooling in ice water bath at 0 deg.C, centrifuging, collecting the supernatant, vacuum freeze drying, and collecting to obtain repair peptide 2.
Preparation example 9
Preparation of repair complex peptide
(1) Taking 0.1 part of wheat protein, placing the wheat protein into 15 parts of deionized water, stirring and collecting to obtain a suspension;
(2) adjusting the pH of the suspension to 5.5 by using 0.1mol/L sodium hydroxide solution or 0.1mol/L hydrochloric acid, adding mixed enzyme prepared by mixing pepsin and trypsin with equal mass, and performing enzymolysis treatment by controlling the addition amount to be 50 u/g;
(3) collecting the suspension after enzymolysis, heating to 110 deg.C, boiling, inactivating enzyme, cooling in ice water bath at 0 deg.C, centrifuging, collecting the supernatant, vacuum freeze drying, and collecting to obtain repair peptide 3.
Examples
Example 1
Preparing a high-activity biological agent for removing the parasite toxin of the pet:
s1, preparation of probiotic bacteria agent: firstly, according to the mass ratio of bacillus liquid to lactobacillus liquid to saccharomycete liquid of 2: 3: 1, preparing a composite probiotic bacterial liquid, mixing 45 parts of the composite probiotic bacterial liquid with 30 parts of the freeze-drying protective carrier 1, stirring, grinding and dispersing, collecting dispersed slurry, performing vacuum freeze drying, and collecting a probiotic bacterial agent;
s2, blending treatment: and then stirring and mixing 3 parts of polyvinyl alcohol solution and the probiotic agent, crushing and sieving after vacuum freeze drying, and collecting the high-activity biological agent 1 for removing the pet parasitic toxin.
Example 2
Preparing a high-activity biological agent for removing the parasite toxin of the pet:
s1, preparation of probiotic bacteria agent: firstly, according to the mass ratio of bacillus liquid to lactobacillus liquid to saccharomycete liquid of 2: 3: 1, preparing a composite probiotic bacterial liquid, mixing 45 parts of the composite probiotic bacterial liquid with 27.5 parts of a freeze-drying protective carrier 1, stirring, grinding and dispersing, collecting dispersed slurry, performing vacuum freeze drying, and collecting a probiotic bacterial agent;
s2, blending treatment: and then stirring and mixing 3 parts of polyvinyl alcohol solution and the probiotic agent, crushing and sieving after vacuum freeze drying, and collecting the high-activity biological agent 2 for removing the pet parasitic toxin.
Example 3
Preparing a high-activity biological agent for removing the parasite toxin of the pet:
s1, preparation of probiotic bacteria agent: firstly, according to the mass ratio of bacillus liquid to lactobacillus liquid to saccharomycete liquid of 2: 3: 1, preparing a composite probiotic bacterial liquid, mixing 50 parts of the composite probiotic bacterial liquid with 30 parts of the freeze-drying protective carrier 1, stirring, grinding and dispersing, collecting dispersed slurry, and then carrying out vacuum freeze drying to collect a probiotic bacterial agent;
s2, blending treatment: and stirring and mixing 4 parts of polyvinyl alcohol solution and the probiotic agent, freezing and drying in vacuum, crushing and sieving, and collecting the high-activity biological agent 3 for removing the parasitic toxins of the pets.
Example 4
Preparing a high-activity biological agent for removing the parasite toxin of the pet:
s1, preparation of probiotic bacteria agent: firstly, according to the mass ratio of bacillus liquid to lactobacillus liquid to saccharomycete liquid of 2: 3: 1, preparing a composite probiotic bacterial liquid, mixing 47.5 parts of the composite probiotic bacterial liquid with 25 parts of a freeze-drying protective carrier 2, stirring, grinding and dispersing, collecting dispersed slurry, performing vacuum freeze drying, and collecting a probiotic bacterial agent;
s2, blending treatment: and stirring and mixing the 3 parts of polyvinyl alcohol solution and the probiotic agent, freezing and drying in vacuum, crushing and sieving, and collecting the high-activity biological agent 4 for removing the parasitic toxins of the pets.
Example 5
Preparing a high-activity biological agent for removing the parasite toxin of the pet:
s1, preparation of probiotic bacteria agent: firstly, according to the mass ratio of bacillus liquid to lactobacillus liquid to saccharomycete liquid of 2: 3: 1, preparing a composite probiotic bacterial liquid, mixing 47.5 parts of the composite probiotic bacterial liquid with 30 parts of a freeze-drying protective carrier 2, stirring, grinding and dispersing, collecting dispersed slurry, performing vacuum freeze drying, and collecting a probiotic bacterial agent;
s2, blending treatment: and stirring and mixing 4 parts of polyvinyl alcohol solution and the probiotic agent, freezing and drying in vacuum, crushing and sieving, and collecting to obtain the high-activity biological agent 5 for removing the parasitic toxins of the pets.
Example 6
Preparing a high-activity biological agent for removing the parasite toxin of the pet:
s1, preparation of probiotic bacteria agent: firstly, according to the mass ratio of bacillus liquid to lactobacillus liquid to saccharomycete liquid of 2: 3: 1, preparing a composite probiotic bacterial liquid, mixing 50 parts of the composite probiotic bacterial liquid with 25 parts of a freeze-drying protective carrier 3, stirring, grinding and dispersing, collecting dispersed slurry, and then carrying out vacuum freeze drying to collect a probiotic bacterial agent;
s2, blending treatment: and stirring and mixing 5 parts of polyvinyl alcohol solution and the probiotic agent, freezing and drying in vacuum, crushing and sieving, and collecting the high-activity biological agent 6 for removing the parasitic toxins of the pets.
Examples 7 to 9
The difference between the high-activity biological preparation for removing the pet parasitic toxins and the embodiment 1 is that the embodiment 7-9 is further provided with a premixing treatment step in the preparation of the high-activity biological preparation for removing the pet parasitic toxins, in the premixing treatment step, the porous polypeptide slow-release carriers 1-3 are respectively mixed with probiotic bacteria agents to prepare the high-activity biological preparation for removing the pet parasitic toxins, and the premixing treatment step is as follows:
example 7: s2, pre-mixing treatment: stirring and mixing 45 parts of the porous polypeptide slow-release carrier 1 and the probiotic microbial inoculum, and collecting to obtain the premix 1.
Example 8: s2, pre-mixing treatment: 47 parts of the porous polypeptide slow-release carrier 2 and the probiotic microbial inoculum are stirred and mixed, and the premix 2 is obtained by collection.
Example 9: s2, pre-mixing treatment: stirring and mixing 50 parts of the porous polypeptide slow-release carrier 3 and the probiotic microbial inoculum, and collecting to obtain a premix 3.
Examples 10 to 12
The difference between the high-activity biological preparation for removing the pet parasitic toxins and the embodiment 7 is that in the embodiment 10-12, in the preparation of the high-activity biological preparation for removing the pet parasitic toxins, a premixing treatment step is further provided, in the premixing treatment step, the porous polypeptide slow-release carrier 1 and the repair composite peptides 1-3 are respectively mixed with a probiotic microbial inoculum to prepare the high-activity biological preparation for removing the pet parasitic toxins, and the premixing treatment step is as follows:
example 10: s2, pre-mixing treatment: stirring and mixing 45 parts of porous polypeptide slow-release carrier 1, 6 parts of repair composite peptide 1 and probiotic bacteria agent, and collecting to obtain premix 4.
Example 11: s2, pre-mixing treatment: stirring and mixing 45 parts of porous polypeptide slow-release carrier 1, 7 parts of repair composite peptide 2 and probiotic bacteria agent, and collecting to obtain premix 5.
Example 12: s2, pre-mixing treatment: stirring and mixing 45 parts of porous polypeptide slow-release carrier 1, 8 parts of repair composite peptide 3 and probiotic bacteria agent, and collecting to obtain premix 6.
Examples 13 to 16
The difference between the high-activity biological preparation for removing the pet parasitic toxins and the embodiment 10 is that in the embodiment 13-16, a premixing treatment step is further provided in the preparation of the high-activity biological preparation for removing the pet parasitic toxins, and in the premixing treatment step, the porous polypeptide slow-release carrier 1, the repair complex peptide 1 and the probiotic microbial inoculum are respectively mixed to prepare the high-activity biological preparation for removing the pet parasitic toxins, wherein the premixing treatment step is as follows:
example 13: s2, pre-mixing treatment: stirring and mixing 45 parts of porous polypeptide slow-release carrier 1, 6 parts of repair composite peptide 1, 3 parts of clove flower essential oil microcapsule and probiotic bacteria agent, and collecting to obtain premix 7.
Example 14: s2, pre-mixing treatment: stirring and mixing 45 parts of porous polypeptide slow-release carrier 1, 6 parts of repair compound peptide 1, 4 parts of marigold essential oil microcapsule and probiotic bacteria agent, and collecting to obtain premix 8.
Example 15: s2, pre-mixing treatment: stirring and mixing 45 parts of porous polypeptide slow-release carrier 1, 6 parts of repair compound peptide 1, 5 parts of marigold essential oil microcapsules and probiotic bacteria agent, and collecting to obtain premix 9.
Example 16: s2, pre-mixing treatment: stirring and mixing 45 parts of porous polypeptide slow-release carrier 1, 6 parts of repair compound peptide 1, 4 parts of impatiens balsamina essential oil microcapsule and probiotic bacteria agent, and collecting to obtain premix 10.
Comparative example
Comparative example 1: compared with the example 1, the high-activity biological preparation for removing the pet parasite toxin does not adopt a freeze-drying protective carrier, and the rest components and the preparation steps are the same as the example 1.
Comparative example 2: compared with the example 1, the high-activity biological preparation for removing the parasite toxin of the pet does not add the compound probiotic bacteria liquid, and the other components and the preparation steps are the same as the example 1.
Performance test
For the convenience of testing, the application only performs performance detection on the high-activity biological preparations for removing the pet parasite toxin prepared in the examples 1-16 and the comparative examples 1-2 so as to detect the performance of the finished high-activity biological preparation for removing the pet parasite toxin.
Detection method/test method
19 adult cats which just have been subjected to insect expelling and pest expelling and meet the pet experimental standard are respectively used as experimental cats, 19 adult dogs which just have been subjected to insect expelling and pest expelling and meet the pet experimental standard are respectively used as experimental dogs, each group of pets comprises 1 dog and 1 cat, and the total number of the groups of animals is 19.
The temperature of the breeding room is 23 +/-2 ℃, and the relative humidity is 55 +/-5%. Selecting a group of animal groups, wherein no substance is added in the basic cat food and the basic dog food to serve as a control group 1; the high activity biological agents for removing pet parasite toxins prepared in examples 1 to 16 and comparative examples 1 to 2, which respectively account for 5% by weight of the basic cat food and the basic dog food, were added to the remaining 15 groups of the basic cat food and the basic dog food as experimental groups 1 to 18. Two identical sets of pots were provided for each group of animals. Feeding dogs regularly, feeding cats freely for 12 hours, weighing again after feeding, and recording the weight of the rest animal food of the control group 1 and the experimental groups 1-18; feeding 1 meal every day, and continuously feeding for 7 days; before feeding, the tableware is cleaned, the drinking water is prepared, and the tableware is fixed to prevent overturn. When fed, the animal has good health condition and no vomiting and diarrhea symptoms. The weight gain and loss ratios of the control group 1 and the experimental groups 1 to 18 were recorded.
The results of the experiment are listed in table 1 below:
TABLE 1 data table of weight loss ratios of examples 1 to 16 and comparative examples 1 to 2
Firstly, 4 groups of schemes are set for the embodiments 1 to 6, 7 to 9, 10 to 12 and 13 to 16, and the performance analysis is carried out by combining the comparative examples 1 to 2, the comparison group 1 and the performance table in the table 1, wherein the specific results are as follows:
(1) according to the data in the table 1 and the technical schemes in the embodiments 1-6 and the comparative examples 1-2 and the control group 1, the weight of the pet in the comparative examples 1-2 and the control group is not increased or decreased, but the weight of the pet in the embodiments 1-6 is increased, which indicates that the complex probiotic bacterial liquid is selected to treat the parasitic toxin in the pet body and repair the internal environment of the pet body, and the parasitic toxin can destroy intestinal mucosa in the pet body greatly, so that the complex probiotic bacterial liquid is fixed on the intestinal mucosa through the repair effect of the complex probiotic bacterial liquid, and further erosion of the intestinal mucosa by the parasitic toxin is prevented. Secondly, the composite probiotics existing in the intestinal tract can be effectively distributed in different regions of the intestinal tract to form stable protection regions, so that bacteriocin is released, the invasion of competitors is inhibited, and the effects of repairing the intestinal mucosa of the pet and removing the parasite toxin in the pet are achieved.
(2) And comparing the weight of the pets in the examples 7 to 9 with that in the example 1 and the examples 7 to 9, the weight of the pets in the examples 7 to 9 is obviously increased, which shows that the application adopts the polypeptide slow-release material as the carrier, effectively prolongs the retention time of the composite probiotic biological preparation in the gastrointestinal tract, and further improves the loading capacity of the polypeptide slow-release carrier to the active ingredients of the biological preparation, thereby effectively improving the activity and the repairing effect of the biological preparation.
(3) Comparing examples 10-12 with example 7, it is demonstrated that the repairing composite peptide formed by enzymolysis of millet protein can be rapidly decomposed into glycyl glutamine in vivo, and the substance is the only energy source of small intestinal mucosa cells and the most important nutrient substance for intestinal tract repair, so that intestinal mucosa can be effectively repaired, intestinal mucosa atrophy is prevented, intestinal immunity is improved, weight, structure and protein content of intestinal normal mucosa are maintained, and parasitic toxin is effectively eliminated to a certain extent, thereby effectively improving pet constitution and function.
(4) Finally, by comparing with examples 10 and 13 to 16, it is demonstrated that the main chemical components of the preferred clove flower essential oil, marigold essential oil, balm flower essential oil or balsamine essential oil of the present application are plant polyphenols which have strong inhibitory effect on harmful mixed bacteria such as candida albicans, aspergillus, dermatophytosis and the like, and the inhibitory effect of the high-activity biological agent on the harmful mixed bacteria is improved.
The present embodiment is only for explaining the present application, and it is not limited to the present application, and those skilled in the art can make modifications of the present embodiment without inventive contribution as needed after reading the present specification, but all of them are protected by patent law within the scope of the claims of the present application.
Claims (9)
1. A high-activity biological agent for removing parasite toxins of pets is characterized by comprising the following substances in parts by weight:
45-50 parts of composite probiotic bacteria liquid;
25-30 parts of a freeze-drying protective carrier;
3-5 parts of a polyvinyl alcohol solution;
the freeze-drying protection carrier comprises the following substances in parts by weight:
3-5 parts of glucose;
3-5 parts of lactose;
25-30 parts of protein powder;
15-30 parts of water.
2. The high-activity biological agent for removing the pet parasitic toxins according to claim 1, wherein the compound probiotic bacterial liquid is prepared by mixing bacillus bacterial liquid, lactobacillus bacterial liquid and yeast bacterial liquid, and the mass ratio of the bacillus bacterial liquid to the lactobacillus bacterial liquid to the yeast bacterial liquid is 2: 3: 1.
3. the high-activity biological agent for removing the pet parasitic toxin according to claim 1, characterized by further comprising 45-50 parts of a slow release carrier, wherein the slow release carrier is a porous polypeptide slow release carrier, and the porosity of the porous polypeptide slow release carrier is 45-50%.
4. The highly active biological agent for removing pet parasite toxins according to claim 3, wherein the porous polypeptide sustained release carrier is prepared by the following scheme:
(1) stirring and mixing the protein polypeptide and the sodium alginate solution according to the mass ratio of 1: 3-1: 5, collecting the mixed solution and placing the mixed solution in a spray drying device;
(2) spraying and pressing the particles generated by drying in a spray drying device into a calcium chloride solution, stirring and crosslinking after the spraying and pressing are finished, and collecting a crosslinking mixed solution;
(3) and filtering the crosslinking mixed solution, taking a filter cake, washing with deionized water, carrying out vacuum freeze drying, and collecting the porous polypeptide slow-release carrier.
5. The high-activity biological agent for removing the pet parasite toxin according to claim 3, wherein the high-activity biological agent further comprises 6-8 parts of repair complex peptide, and the repair complex peptide is prepared by adopting the following scheme:
(1) placing wheat protein in deionized water, stirring and collecting to obtain a suspension;
(2) adjusting the pH of the suspension to 5.5 by using a sodium hydroxide solution or hydrochloric acid, and adding protease for enzymolysis;
(3) collecting the suspension after enzymolysis, heating, boiling, inactivating enzyme, cooling in ice water bath, centrifuging, collecting the supernatant, vacuum freeze drying, and collecting to obtain the repair peptide.
6. The highly active biological agent for removing pet parasite toxins according to claim 5, wherein the protease comprises mixed equal mass pepsin and trypsin.
7. The highly active biological agent for removing pet parasite toxins according to claim 5, wherein the highly active biological agent further comprises 3-5 parts of plant essential oil microcapsules.
8. The highly active biological agent for removing pet parasite toxins according to claim 7, wherein the plant essential oil comprises any one or more of flos Caryophylli essential oil, flos Tagetis Erectae essential oil, Vespa velutina essential oil or flos Impatientis essential oil.
9. A preparation method of a high-activity biological agent for removing pet parasite toxins is characterized by comprising the following preparation steps:
s1, preparation of probiotic bacteria agent: firstly, mixing the composite probiotic bacteria liquid with a freeze-drying protective carrier, stirring, grinding and dispersing, collecting dispersed slurry, then carrying out vacuum freeze drying, and collecting the probiotic bacteria agent;
s2, pre-mixing treatment: stirring and mixing the plant essential oil microcapsule, the repair compound peptide and the probiotic agent, and collecting to obtain a premix;
s3, blending treatment: and stirring and mixing the uniformly mixed material, the polyvinyl alcohol solution and the porous polypeptide slow-release carrier, crushing and sieving after vacuum freeze drying, and collecting the high-activity biological preparation for removing the pet parasitic toxin.
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