CN102283334A - Complex probiotic microcapsule capable of improving intestinal tract micro-ecology of small-sized dogs - Google Patents
Complex probiotic microcapsule capable of improving intestinal tract micro-ecology of small-sized dogs Download PDFInfo
- Publication number
- CN102283334A CN102283334A CN2011102538763A CN201110253876A CN102283334A CN 102283334 A CN102283334 A CN 102283334A CN 2011102538763 A CN2011102538763 A CN 2011102538763A CN 201110253876 A CN201110253876 A CN 201110253876A CN 102283334 A CN102283334 A CN 102283334A
- Authority
- CN
- China
- Prior art keywords
- complex probiotic
- complex
- compound probiotic
- fermentation
- small
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention discloses a complex probiotic microcapsule capable of improving an intestinal tract micro-ecology of small-sized dogs. The production method of the microcapsule comprises the following steps of: A1, preparing a complex microbial inoculum; A2, preparing complex probiotic solution; A3, mixing 2-5% of porous starch with the complex probiotic solution and adjusting the pH value to 6.0; controlling the temperature in a range of 25-35 DEG C and shaking for 30 min; after the materials are completely adsorbed, orderly adding the following outer layer embedded materials according to weight percentages: 1-3% of sodium alginate, 1-5% of gelatin and 1-5% of dextrin; uniformly agitating and dripping calcium chloride solution to solidify; freezing and drying to prepare the complex probiotic microcapsule. The complex probiotic microcapsule provided by the invention takes the porous starch as a core material for adsorbing the complex probiotic solution and then takes the sodium alginate, the gelatin and the dextrin as wall materials to process to obtain the complex probiotic microcapsule.
Description
Technical field
The present invention relates to a kind of compound probiotic microcapsules that improve small-sized dog intestinal microecology, especially through the compound probiotic microcapsules of the small-sized dog intestinal microecology of improving of porous starch embedding.
Background technology
In China, along with the raising of living standards of the people, pet owners are also more and more paid attention to the quality of life of pet.After initial stage raising blindly, increasing pet owners begins to pay attention to the health problem of pet, and various pet care product have also entered pet family.But because domestic research starting evening in the industry, be specifically designed at present pet dog particularly the homemade probiotics preparation of small-sized dog mostly be single microbial inoculum.
Probio is the active microorganism that plays a role by the balance of improving host enteric microorganism flora.Can improve the balance of host enteric microorganism behind this microorganism oral administration sufficient amount, and then host health is produced useful influence.But there are problems such as shelf life is short, viable bacteria concentration is not up to standard in existing probiotic products.
Porous-starch is a kind of novel converted starch, has good heat resistance, hardness is big and security is good characteristics, is a kind of desirable insoluble carrier that is suitable for the probio embedding.
Summary of the invention
Technical problem to be solved by this invention is to provide a kind of compound probiotic microcapsules through the small-sized dog intestinal microecology of improving of porous starch embedding at the deficiencies in the prior art.
A kind of compound probiotic microcapsules that improve small-sized dog intestinal microecology, its preparation method may further comprise the steps:
A1, will through lactobacillus acidophilus, Lactobacillus plantarum and Lactobacillus rhamnosus that strain passage, bacterial classification co-induction filter out respectively on the nutrient agar in 35～40 ℃ cultivate 35～45h after, 2～4 ℃ of refrigerators are preserved standby; Took by weighing above-mentioned three bacterial classifications by weight 2: 1: 2, the inoculum concentration with 3～5% is inoculated in through in the culture medium of steam sterilizing, adjusts pH value to 6.5～7.3, puts into fermentation tank cultivation and fermentation 15～20h, obtains composite bacteria agent capable;
A2 adds composite bacteria agent capable and puts into airtight jar after solid medium mixes and ferment, and fermentation temperature is 35～37 ℃, and fermentation time is 8～10d, ferments synchronously, and finally obtaining viable count is 1.00～3.5 * 10
9The compound probiotic liquid of CFU/mL;
A3, porous-starch with 2～5% mixes with compound probiotic liquid, the pH value transfers to 6.0,25～35 ℃ of vibrations of temperature control 30min after treating fully to adsorb, adds following outer embedded material according to percentage by weight: 1～3% sodium alginate, 1～5% gelatin, 1～5% dextrin successively, stir, splash into calcium chloride solution and solidify, freeze drying makes the compound probiotic microcapsules.
The present invention is core with the porous-starch, and absorption compound probiotic liquid is the wall material with sodium alginate, dextrin and gelatin again, processing compound probiotic microcapsules.
The specific embodiment
Below in conjunction with instantiation, the present invention is described in detail.
The used culture medium of the present invention is conventional culture medium or recommends culture medium.
(1) at first, with lactobacillus acidophilus (Lactobacillus acidophilus, purchase is from CICC, deposit number 6075), Lactobacillus plantarum (Lactobacillus plantarum, purchase is from CICC, deposit number 22696) and Lactobacillus rhamnosus (Lactobacillus rhamnosus buys from CICC, deposit number 6135) after strain passage, the screening of bacterial classification co-induction, adjusted language order herein.Respectively on the nutrient agar in 35～40 ℃ cultivate 35～45h after, 2～4 ℃ of refrigerators are preserved standby; Took by weighing above-mentioned three bacterial classifications by weight 2: 1: 2, the inoculum concentration with 3～5% is inoculated in through in the culture medium of steam sterilizing, adjusts pH value to 6.5～7.3, puts into fermentation tank cultivation and fermentation 15～20h, obtains composite bacteria agent capable.
Composite bacteria agent capable added put into airtight jar after solid medium mixes and ferment, fermentation temperature is 35～37 ℃, and fermentation time is 8～10d, ferments synchronously, and finally obtaining viable count is 1.00～3.5 * 10
9The composite bacteria liquid of CFU/mL.
(2) porous-starch with 2～5% (percentage by weights) mixes with compound probiotic liquid, the pH value transfers to 6.0,25～35 ℃ of vibrations of temperature control 30min, after treating fully absorption, add following outer embedded material successively according to percentage by weight: 1～3% sodium alginate, 1～5% gelatin, 1～5% dextrin, stir, splash into calcium chloride solution and solidify, freeze drying makes the compound probiotic microcapsules.
(3) actual embedding viable count calculates: the compound probiotic microcapsules are dissolved in the phosphate buffer, add AMS and stir 30～60min, carry out count plate after treating to dissolve fully; Directly, carry out count plate with physiological saline solution compound probiotic surface of microcapsule thalline.Twice measured difference is actual embedding viable count 0.56～1.24 * 10
9CFU/mL.
(4) feeding experiment: select 18 of small-sized dogs, be divided into 3 groups at random, divide into groups, weigh.Test is carried out in same environment, and the management expectancy unanimity of feeding is observed a dog ight soil situation every day: color, state, smell.Attack behind the bacterium and under aseptic condition, to collect fresh excreta 2～3g respectively in 0,3,5,7 day, be sent to the laboratory immediately and detect.Bifidobacterium detects according to GB/T 4789.34-2003, lactic acid bacteria detects according to SN/T 1941.1-2007, Escherichia coli detect according to GB/T 4789.3-2003, C.perfringens detects according to SN 0177-92, and salmonella detects according to GB/T 4789.4-2008.Obtaining result of the test sees the following form.
Table 1 compound probiotic microcapsules are to the influence of flora in the small-sized dog ight soil
The embedding of porous-starch plays a very good protection to thalline, has effectively avoided probio to be subjected to the influence of light, heat, air and chemical environment, for production in enormous quantities, sale and storing provide advantageous conditions.And prove that by feeding experiment the compound probiotic microcapsules have the small-sized dog intestinal microecology environment of improvement and digestant effect.
Should be understood that, for those of ordinary skills, can be improved according to the above description or conversion, and all these improvement and conversion all should belong to the protection domain of claims of the present invention.
Claims (1)
1. compound probiotic microcapsules that can improve small-sized dog intestinal microecology is characterized in that its preparation method may further comprise the steps:
A1, will through lactobacillus acidophilus, Lactobacillus plantarum and Lactobacillus rhamnosus that strain passage, bacterial classification co-induction filter out respectively on the nutrient agar in 35～40 ℃ cultivate 35～45h after, 2～4 ℃ of refrigerators are preserved standby; Took by weighing above-mentioned three bacterial classifications by weight 2: 1: 2, the inoculum concentration with 3～5% is inoculated in through in the culture medium of steam sterilizing, adjusts pH value to 6.5～7.3, puts into fermentation tank cultivation and fermentation 15～20h, obtains composite bacteria agent capable;
A2 adds composite bacteria agent capable and puts into airtight jar after solid medium mixes and ferment, and fermentation temperature is 35～37 ℃, and fermentation time is 8～10d, ferments synchronously, and finally obtaining viable count is 1.00～3.5 * 10
9The compound probiotic liquid of CFU/mL;
A3, the porous-starch with 2～5% mixes with compound probiotic liquid, and the pH value transfers to 6.0,25～35 ℃ of vibrations of temperature control 30min.After treating fully absorption, add following outer embedded material successively according to percentage by weight: 1～3% sodium alginate, 1～5% gelatin, 1～5% dextrin, stir, splash into calcium chloride solution and solidify, freeze drying makes the compound probiotic microcapsules.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011102538763A CN102283334A (en) | 2011-08-31 | 2011-08-31 | Complex probiotic microcapsule capable of improving intestinal tract micro-ecology of small-sized dogs |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011102538763A CN102283334A (en) | 2011-08-31 | 2011-08-31 | Complex probiotic microcapsule capable of improving intestinal tract micro-ecology of small-sized dogs |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN102283334A true CN102283334A (en) | 2011-12-21 |
Family
ID=45330313
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2011102538763A Pending CN102283334A (en) | 2011-08-31 | 2011-08-31 | Complex probiotic microcapsule capable of improving intestinal tract micro-ecology of small-sized dogs |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102283334A (en) |
Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103202423A (en) * | 2013-04-17 | 2013-07-17 | 合肥工业大学 | Fructus trichosanthis polysaccharide composite capsule and preparation method thereof |
| CN103340408A (en) * | 2013-07-26 | 2013-10-09 | 湖南农业大学 | Rice antioxidant peptide microcapsule and preparation method thereof |
| ITMI20121328A1 (en) * | 2012-07-30 | 2014-01-31 | Probiotical Spa | COMPOSITION FOR MEDICAL DEVICE INCLUDING BACTERIA STRAINS PRODUCERS OF ESOPOLISACCHARIDES IN ASSOCIATION WITH GUMS AND / OR GELATINS. |
| CN104928278A (en) * | 2015-07-13 | 2015-09-23 | 通威股份有限公司 | Preparation method for composite bacillus microcapsule preparations |
| CN106491374A (en) * | 2016-12-06 | 2017-03-15 | 广州睿森生物科技有限公司 | A kind of nepetalactone microcapsule and preparation method thereof with preparing the application in mosquito repellent product |
| CN108851086A (en) * | 2018-06-11 | 2018-11-23 | 西北大学 | One kind is with micropore starch for protectant active lactic acid bacteria microcapsule and preparation method |
| CN108936030A (en) * | 2018-05-21 | 2018-12-07 | 沂南帅克宠物用品有限公司 | A kind of pet nutrition composition reducing pet kenosis peculiar smell containing probiotics |
| CN109111294A (en) * | 2018-10-24 | 2019-01-01 | 安文彬 | A kind of organic microbial complex ecological fertilizer and preparation method thereof |
| CN112514918A (en) * | 2020-12-23 | 2021-03-19 | 湖南恒圣创新建筑科技有限公司 | Embedding body composite insecticidal probiotic agent and preparation method and application thereof |
| CN113729112A (en) * | 2021-11-05 | 2021-12-03 | 山东健源生物科技有限公司 | High-activity biological agent for removing pet parasitic toxin and preparation method thereof |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA2654457A1 (en) * | 2006-06-06 | 2007-12-13 | Mcgill University | Fermented milk product and use thereof |
-
2011
- 2011-08-31 CN CN2011102538763A patent/CN102283334A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA2654457A1 (en) * | 2006-06-06 | 2007-12-13 | Mcgill University | Fermented milk product and use thereof |
Non-Patent Citations (2)
| Title |
|---|
| 胡兆伟等: "活性乳酸菌微胶囊制备工艺的优化", 《农产品加工.学刊》 * |
| 陈红霞等: "《食品微生物学及实验技术》", 31 August 2008, 化学工业出版社 * |
Cited By (16)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US9889166B2 (en) | 2012-07-30 | 2018-02-13 | Probiotical S.P.A. | Treating low production of mucus in the gastrointestinal tract with S. thermophilus |
| CN104540512A (en) * | 2012-07-30 | 2015-04-22 | 益生菌股份公司 | Compositions for a medical device comprising exopolysaccharide-producing bacterial strains in association with gums and/or gelatines |
| ITMI20121328A1 (en) * | 2012-07-30 | 2014-01-31 | Probiotical Spa | COMPOSITION FOR MEDICAL DEVICE INCLUDING BACTERIA STRAINS PRODUCERS OF ESOPOLISACCHARIDES IN ASSOCIATION WITH GUMS AND / OR GELATINS. |
| WO2014020408A1 (en) * | 2012-07-30 | 2014-02-06 | Probiotical S.P.A. | Compositions for a medical device comprising exopolysaccharide-producing bacterial strains in association with gums and/or gelatines |
| CN103202423A (en) * | 2013-04-17 | 2013-07-17 | 合肥工业大学 | Fructus trichosanthis polysaccharide composite capsule and preparation method thereof |
| CN103340408B (en) * | 2013-07-26 | 2015-10-28 | 湖南农业大学 | A kind of rice antioxidant peptide microcapsule and preparation method thereof |
| CN103340408A (en) * | 2013-07-26 | 2013-10-09 | 湖南农业大学 | Rice antioxidant peptide microcapsule and preparation method thereof |
| CN104928278A (en) * | 2015-07-13 | 2015-09-23 | 通威股份有限公司 | Preparation method for composite bacillus microcapsule preparations |
| CN106491374A (en) * | 2016-12-06 | 2017-03-15 | 广州睿森生物科技有限公司 | A kind of nepetalactone microcapsule and preparation method thereof with preparing the application in mosquito repellent product |
| CN106491374B (en) * | 2016-12-06 | 2020-01-10 | 广州睿森生物科技有限公司 | Nepetalactone microcapsule, preparation method thereof and application of nepetalactone microcapsule in preparation of mosquito repellent product |
| CN108936030A (en) * | 2018-05-21 | 2018-12-07 | 沂南帅克宠物用品有限公司 | A kind of pet nutrition composition reducing pet kenosis peculiar smell containing probiotics |
| CN108851086A (en) * | 2018-06-11 | 2018-11-23 | 西北大学 | One kind is with micropore starch for protectant active lactic acid bacteria microcapsule and preparation method |
| CN109111294A (en) * | 2018-10-24 | 2019-01-01 | 安文彬 | A kind of organic microbial complex ecological fertilizer and preparation method thereof |
| CN112514918A (en) * | 2020-12-23 | 2021-03-19 | 湖南恒圣创新建筑科技有限公司 | Embedding body composite insecticidal probiotic agent and preparation method and application thereof |
| CN113729112A (en) * | 2021-11-05 | 2021-12-03 | 山东健源生物科技有限公司 | High-activity biological agent for removing pet parasitic toxin and preparation method thereof |
| CN113729112B (en) * | 2021-11-05 | 2022-02-18 | 山东健源生物科技有限公司 | High-activity biological agent for removing pet parasitic toxin and preparation method thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102283334A (en) | Complex probiotic microcapsule capable of improving intestinal tract micro-ecology of small-sized dogs | |
| CN106399196B (en) | One lactobacillus plantarum HEW-A490 and its application | |
| CN103820363B (en) | A kind of preparation and application of faecium bacterium powder | |
| CN101338283B (en) | Lactobacillus casei and applications thereof in solid-state fermentation | |
| WO2017012570A1 (en) | Bacillus coagulans, high cell density fermentation method thereof, and method for preparing spray-dried powder thereof | |
| CN103704719B (en) | Preparation method of probiotic microcapsule with high viable count | |
| CN107312732B (en) | Probiotic feed additive | |
| CN107287136A (en) | A kind of preparation method of the prebiotic solid lactic acid bacteria agent with prebiotic function | |
| CN107164274A (en) | A kind of lactic acid bacteria composite fungicide and its preparation method and application | |
| CN102370057A (en) | Lactobacillus microcapsule and preparation method thereof | |
| CN104651268A (en) | Lactobacillus plantarum and application thereof | |
| CN113604382A (en) | Lactobacillus paracasei YT170 and application thereof | |
| CN105132321B (en) | A kind of VREF and the culture medium of high density solid state fermentation thereof and method | |
| CN115094012B (en) | Preparation method and application of bacillus coagulans BC-HYC strain microbial inoculum | |
| CN104371960A (en) | Compound fungicide and continuous fermentation method of compound microorganisms adopted | |
| CN107177522A (en) | One plant height activity forage plant lactobacillus and its cultural method and application | |
| CN109717481A (en) | A kind of preparation process of coating probiotics | |
| WO2017012571A1 (en) | Use of bacillus coagulans strain in increasing egg production in laying hen | |
| CN103300208A (en) | Production method of live lactobacillus casei preparation | |
| CN101884394B (en) | Composite microbial feed additive for chicken and preparation method thereof | |
| CN103283948A (en) | Bifidobacterium bifidum-oriented microecological preparation | |
| CN102907585B (en) | A kind of middle pig probiotic vitamin premix and batch | |
| CN110804571B (en) | Compound lactobacillus preparation and application thereof in preparing feed additive | |
| CN103289983A (en) | Microencapsulated Enterococcus faecium live bacterium preparation and its preparation method | |
| Nedelcheva et al. | Probiotic strain Lactobacillus plantarum NBIMCC 2415 with antioxidant activity as a starter culture in the production of dried fermented meat products |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C12 | Rejection of a patent application after its publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20111221 |