CN113718007A - Preparation method and process flow of small molecule compound peptide - Google Patents
Preparation method and process flow of small molecule compound peptide Download PDFInfo
- Publication number
- CN113718007A CN113718007A CN202111030760.3A CN202111030760A CN113718007A CN 113718007 A CN113718007 A CN 113718007A CN 202111030760 A CN202111030760 A CN 202111030760A CN 113718007 A CN113718007 A CN 113718007A
- Authority
- CN
- China
- Prior art keywords
- preparation
- small molecule
- fresh
- peptide
- process flow
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 108090000765 processed proteins & peptides Proteins 0.000 title claims abstract description 70
- -1 small molecule compound Chemical class 0.000 title claims abstract description 46
- 238000002360 preparation method Methods 0.000 title claims abstract description 39
- 230000008569 process Effects 0.000 title claims abstract description 21
- 230000000694 effects Effects 0.000 claims abstract description 36
- 210000000988 bone and bone Anatomy 0.000 claims abstract description 32
- 241000251511 Holothuroidea Species 0.000 claims abstract description 29
- 239000007788 liquid Substances 0.000 claims abstract description 13
- 238000000034 method Methods 0.000 claims abstract description 13
- 239000000843 powder Substances 0.000 claims abstract description 12
- 108010010803 Gelatin Proteins 0.000 claims description 27
- 229920000159 gelatin Polymers 0.000 claims description 27
- 239000008273 gelatin Substances 0.000 claims description 27
- 235000019322 gelatine Nutrition 0.000 claims description 27
- 235000011852 gelatine desserts Nutrition 0.000 claims description 27
- 239000000243 solution Substances 0.000 claims description 21
- 102000004190 Enzymes Human genes 0.000 claims description 17
- 108090000790 Enzymes Proteins 0.000 claims description 17
- 229940088598 enzyme Drugs 0.000 claims description 17
- 239000000047 product Substances 0.000 claims description 17
- 150000003384 small molecules Chemical class 0.000 claims description 16
- 239000002131 composite material Substances 0.000 claims description 13
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 claims description 9
- 239000012263 liquid product Substances 0.000 claims description 9
- 238000000926 separation method Methods 0.000 claims description 9
- 235000014347 soups Nutrition 0.000 claims description 9
- 230000001954 sterilising effect Effects 0.000 claims description 9
- 235000015097 nutrients Nutrition 0.000 claims description 7
- 238000004806 packaging method and process Methods 0.000 claims description 7
- 239000002994 raw material Substances 0.000 claims description 7
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 7
- 102000057297 Pepsin A Human genes 0.000 claims description 6
- 108090000284 Pepsin A Proteins 0.000 claims description 6
- 102000004142 Trypsin Human genes 0.000 claims description 6
- 108090000631 Trypsin Proteins 0.000 claims description 6
- 239000011259 mixed solution Substances 0.000 claims description 6
- 229940111202 pepsin Drugs 0.000 claims description 6
- 239000012588 trypsin Substances 0.000 claims description 6
- 230000006837 decompression Effects 0.000 claims description 4
- 238000004108 freeze drying Methods 0.000 claims description 4
- 238000007710 freezing Methods 0.000 claims description 4
- 230000008014 freezing Effects 0.000 claims description 4
- 238000009835 boiling Methods 0.000 claims description 3
- 238000004140 cleaning Methods 0.000 claims description 3
- 238000010411 cooking Methods 0.000 claims description 3
- 238000001816 cooling Methods 0.000 claims description 3
- 238000005520 cutting process Methods 0.000 claims description 3
- 230000009849 deactivation Effects 0.000 claims description 3
- 238000007599 discharging Methods 0.000 claims description 3
- 239000000796 flavoring agent Substances 0.000 claims description 3
- 235000019634 flavors Nutrition 0.000 claims description 3
- 238000010438 heat treatment Methods 0.000 claims description 3
- 230000000415 inactivating effect Effects 0.000 claims description 3
- 230000005415 magnetization Effects 0.000 claims description 3
- 238000002791 soaking Methods 0.000 claims description 3
- 238000004659 sterilization and disinfection Methods 0.000 claims description 3
- 238000005406 washing Methods 0.000 claims description 3
- 238000005303 weighing Methods 0.000 claims description 3
- 238000003756 stirring Methods 0.000 claims description 2
- 230000003647 oxidation Effects 0.000 abstract description 5
- 238000007254 oxidation reaction Methods 0.000 abstract description 5
- 150000001875 compounds Chemical class 0.000 abstract description 3
- 102000004169 proteins and genes Human genes 0.000 abstract description 3
- 108090000623 proteins and genes Proteins 0.000 abstract description 3
- 210000001519 tissue Anatomy 0.000 abstract description 3
- 241000283690 Bos taurus Species 0.000 abstract description 2
- 102000008186 Collagen Human genes 0.000 abstract description 2
- 108010035532 Collagen Proteins 0.000 abstract description 2
- 102000006587 Glutathione peroxidase Human genes 0.000 abstract description 2
- 108700016172 Glutathione peroxidases Proteins 0.000 abstract description 2
- 102000019197 Superoxide Dismutase Human genes 0.000 abstract description 2
- 108010012715 Superoxide dismutase Proteins 0.000 abstract description 2
- 238000010521 absorption reaction Methods 0.000 abstract description 2
- 230000003078 antioxidant effect Effects 0.000 abstract description 2
- 229920001436 collagen Polymers 0.000 abstract description 2
- 230000003859 lipid peroxidation Effects 0.000 abstract description 2
- 210000004369 blood Anatomy 0.000 description 17
- 239000008280 blood Substances 0.000 description 17
- 239000003814 drug Substances 0.000 description 9
- 235000013305 food Nutrition 0.000 description 9
- 210000000952 spleen Anatomy 0.000 description 8
- 230000036039 immunity Effects 0.000 description 7
- HVYWMOMLDIMFJA-DPAQBDIFSA-N cholesterol Chemical compound C1C=C2C[C@@H](O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H]([C@H](C)CCCC(C)C)[C@@]1(C)CC2 HVYWMOMLDIMFJA-DPAQBDIFSA-N 0.000 description 6
- 230000007812 deficiency Effects 0.000 description 6
- 239000000463 material Substances 0.000 description 6
- 230000001737 promoting effect Effects 0.000 description 6
- 230000001105 regulatory effect Effects 0.000 description 6
- 206010012735 Diarrhoea Diseases 0.000 description 5
- 240000001417 Vigna umbellata Species 0.000 description 5
- 230000032683 aging Effects 0.000 description 5
- 235000007215 black sesame Nutrition 0.000 description 5
- 230000006870 function Effects 0.000 description 5
- 102000004196 processed proteins & peptides Human genes 0.000 description 5
- 230000008961 swelling Effects 0.000 description 5
- 235000008708 Morus alba Nutrition 0.000 description 4
- 240000000249 Morus alba Species 0.000 description 4
- 235000011453 Vigna umbellata Nutrition 0.000 description 4
- 210000003734 kidney Anatomy 0.000 description 4
- 241000620028 Dolichoris Species 0.000 description 3
- 244000268590 Euryale ferox Species 0.000 description 3
- 244000241838 Lycium barbarum Species 0.000 description 3
- 235000015459 Lycium barbarum Nutrition 0.000 description 3
- 235000015468 Lycium chinense Nutrition 0.000 description 3
- 206010028980 Neoplasm Diseases 0.000 description 3
- 208000027418 Wounds and injury Diseases 0.000 description 3
- 230000009286 beneficial effect Effects 0.000 description 3
- 230000018109 developmental process Effects 0.000 description 3
- 206010012601 diabetes mellitus Diseases 0.000 description 3
- 235000013399 edible fruits Nutrition 0.000 description 3
- 230000002124 endocrine Effects 0.000 description 3
- 150000004676 glycans Chemical class 0.000 description 3
- 210000000936 intestine Anatomy 0.000 description 3
- 235000012054 meals Nutrition 0.000 description 3
- 230000004060 metabolic process Effects 0.000 description 3
- 238000002156 mixing Methods 0.000 description 3
- 239000000203 mixture Substances 0.000 description 3
- 235000016709 nutrition Nutrition 0.000 description 3
- 229920001282 polysaccharide Polymers 0.000 description 3
- 239000005017 polysaccharide Substances 0.000 description 3
- 230000008439 repair process Effects 0.000 description 3
- 210000002784 stomach Anatomy 0.000 description 3
- 241000894006 Bacteria Species 0.000 description 2
- 244000077995 Coix lacryma jobi Species 0.000 description 2
- 235000002722 Dioscorea batatas Nutrition 0.000 description 2
- 235000006536 Dioscorea esculenta Nutrition 0.000 description 2
- 240000001811 Dioscorea oppositifolia Species 0.000 description 2
- 235000003416 Dioscorea oppositifolia Nutrition 0.000 description 2
- 241000196324 Embryophyta Species 0.000 description 2
- 208000008967 Enuresis Diseases 0.000 description 2
- 235000006487 Euryale ferox Nutrition 0.000 description 2
- 108060002716 Exonuclease Proteins 0.000 description 2
- 241000282412 Homo Species 0.000 description 2
- 208000031226 Hyperlipidaemia Diseases 0.000 description 2
- 206010059013 Nocturnal emission Diseases 0.000 description 2
- 208000004880 Polyuria Diseases 0.000 description 2
- 235000008599 Poria cocos Nutrition 0.000 description 2
- 244000197580 Poria cocos Species 0.000 description 2
- 206010041497 Spermatorrhoea Diseases 0.000 description 2
- 241000700605 Viruses Species 0.000 description 2
- 206010052428 Wound Diseases 0.000 description 2
- 150000001413 amino acids Chemical class 0.000 description 2
- 208000007502 anemia Diseases 0.000 description 2
- 206010003549 asthenia Diseases 0.000 description 2
- 235000009508 confectionery Nutrition 0.000 description 2
- 230000035619 diuresis Effects 0.000 description 2
- 229940079593 drug Drugs 0.000 description 2
- 208000001848 dysentery Diseases 0.000 description 2
- 230000002708 enhancing effect Effects 0.000 description 2
- 102000013165 exonuclease Human genes 0.000 description 2
- 235000019197 fats Nutrition 0.000 description 2
- 230000012010 growth Effects 0.000 description 2
- 230000006872 improvement Effects 0.000 description 2
- 239000004615 ingredient Substances 0.000 description 2
- 208000014674 injury Diseases 0.000 description 2
- 210000004185 liver Anatomy 0.000 description 2
- 210000005036 nerve Anatomy 0.000 description 2
- 238000005728 strengthening Methods 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 231100000331 toxic Toxicity 0.000 description 2
- 230000002588 toxic effect Effects 0.000 description 2
- PEYUIKBAABKQKQ-AFHBHXEDSA-N (+)-sesamin Chemical compound C1=C2OCOC2=CC([C@H]2OC[C@H]3[C@@H]2CO[C@@H]3C2=CC=C3OCOC3=C2)=C1 PEYUIKBAABKQKQ-AFHBHXEDSA-N 0.000 description 1
- OYHQOLUKZRVURQ-NTGFUMLPSA-N (9Z,12Z)-9,10,12,13-tetratritiooctadeca-9,12-dienoic acid Chemical compound C(CCCCCCC\C(=C(/C\C(=C(/CCCCC)\[3H])\[3H])\[3H])\[3H])(=O)O OYHQOLUKZRVURQ-NTGFUMLPSA-N 0.000 description 1
- WRIDQFICGBMAFQ-UHFFFAOYSA-N (E)-8-Octadecenoic acid Natural products CCCCCCCCCC=CCCCCCCC(O)=O WRIDQFICGBMAFQ-UHFFFAOYSA-N 0.000 description 1
- BJEPYKJPYRNKOW-REOHCLBHSA-N (S)-malic acid Chemical compound OC(=O)[C@@H](O)CC(O)=O BJEPYKJPYRNKOW-REOHCLBHSA-N 0.000 description 1
- TUSDEZXZIZRFGC-UHFFFAOYSA-N 1-O-galloyl-3,6-(R)-HHDP-beta-D-glucose Natural products OC1C(O2)COC(=O)C3=CC(O)=C(O)C(O)=C3C3=C(O)C(O)=C(O)C=C3C(=O)OC1C(O)C2OC(=O)C1=CC(O)=C(O)C(O)=C1 TUSDEZXZIZRFGC-UHFFFAOYSA-N 0.000 description 1
- LQJBNNIYVWPHFW-UHFFFAOYSA-N 20:1omega9c fatty acid Natural products CCCCCCCCCCC=CCCCCCCCC(O)=O LQJBNNIYVWPHFW-UHFFFAOYSA-N 0.000 description 1
- QSBYPNXLFMSGKH-UHFFFAOYSA-N 9-Heptadecensaeure Natural products CCCCCCCC=CCCCCCCCC(O)=O QSBYPNXLFMSGKH-UHFFFAOYSA-N 0.000 description 1
- 206010067484 Adverse reaction Diseases 0.000 description 1
- 244000144725 Amygdalus communis Species 0.000 description 1
- 235000011437 Amygdalus communis Nutrition 0.000 description 1
- 244000144730 Amygdalus persica Species 0.000 description 1
- 239000004475 Arginine Substances 0.000 description 1
- 206010003210 Arteriosclerosis Diseases 0.000 description 1
- KWIUHFFTVRNATP-UHFFFAOYSA-N Betaine Natural products C[N+](C)(C)CC([O-])=O KWIUHFFTVRNATP-UHFFFAOYSA-N 0.000 description 1
- 244000223760 Cinnamomum zeylanicum Species 0.000 description 1
- 240000007126 Citrus medica var. sarcodactylis Species 0.000 description 1
- 206010010774 Constipation Diseases 0.000 description 1
- 206010011224 Cough Diseases 0.000 description 1
- 240000001008 Dimocarpus longan Species 0.000 description 1
- 235000000235 Euphoria longan Nutrition 0.000 description 1
- 239000001263 FEMA 3042 Substances 0.000 description 1
- 208000004930 Fatty Liver Diseases 0.000 description 1
- 241000628997 Flos Species 0.000 description 1
- 206010017553 Furuncle Diseases 0.000 description 1
- 241000287828 Gallus gallus Species 0.000 description 1
- 235000006200 Glycyrrhiza glabra Nutrition 0.000 description 1
- 244000303040 Glycyrrhiza glabra Species 0.000 description 1
- 240000008669 Hedera helix Species 0.000 description 1
- 235000017443 Hedysarum boreale Nutrition 0.000 description 1
- 235000007858 Hedysarum occidentale Nutrition 0.000 description 1
- 102000001554 Hemoglobins Human genes 0.000 description 1
- 108010054147 Hemoglobins Proteins 0.000 description 1
- 206010019708 Hepatic steatosis Diseases 0.000 description 1
- 206010020751 Hypersensitivity Diseases 0.000 description 1
- 206010020772 Hypertension Diseases 0.000 description 1
- 206010061218 Inflammation Diseases 0.000 description 1
- 208000006877 Insect Bites and Stings Diseases 0.000 description 1
- ODKSFYDXXFIFQN-BYPYZUCNSA-P L-argininium(2+) Chemical compound NC(=[NH2+])NCCC[C@H]([NH3+])C(O)=O ODKSFYDXXFIFQN-BYPYZUCNSA-P 0.000 description 1
- HNDVDQJCIGZPNO-YFKPBYRVSA-N L-histidine Chemical compound OC(=O)[C@@H](N)CC1=CN=CN1 HNDVDQJCIGZPNO-YFKPBYRVSA-N 0.000 description 1
- KDXKERNSBIXSRK-YFKPBYRVSA-N L-lysine Chemical compound NCCCC[C@H](N)C(O)=O KDXKERNSBIXSRK-YFKPBYRVSA-N 0.000 description 1
- KDXKERNSBIXSRK-UHFFFAOYSA-N Lysine Natural products NCCCCC(N)C(O)=O KDXKERNSBIXSRK-UHFFFAOYSA-N 0.000 description 1
- 239000004472 Lysine Substances 0.000 description 1
- 235000006679 Mentha X verticillata Nutrition 0.000 description 1
- 235000002899 Mentha suaveolens Nutrition 0.000 description 1
- 235000001636 Mentha x rotundifolia Nutrition 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- KWIUHFFTVRNATP-UHFFFAOYSA-O N,N,N-trimethylglycinium Chemical compound C[N+](C)(C)CC(O)=O KWIUHFFTVRNATP-UHFFFAOYSA-O 0.000 description 1
- 241000209477 Nymphaeaceae Species 0.000 description 1
- 208000008589 Obesity Diseases 0.000 description 1
- 206010030113 Oedema Diseases 0.000 description 1
- ZQPPMHVWECSIRJ-UHFFFAOYSA-N Oleic acid Natural products CCCCCCCCC=CCCCCCCCC(O)=O ZQPPMHVWECSIRJ-UHFFFAOYSA-N 0.000 description 1
- 239000005642 Oleic acid Substances 0.000 description 1
- 240000004371 Panax ginseng Species 0.000 description 1
- 235000005035 Panax pseudoginseng ssp. pseudoginseng Nutrition 0.000 description 1
- 235000003140 Panax quinquefolius Nutrition 0.000 description 1
- 235000001188 Peltandra virginica Nutrition 0.000 description 1
- LRBQNJMCXXYXIU-PPKXGCFTSA-N Penta-digallate-beta-D-glucose Natural products OC1=C(O)C(O)=CC(C(=O)OC=2C(=C(O)C=C(C=2)C(=O)OC[C@@H]2[C@H]([C@H](OC(=O)C=3C=C(OC(=O)C=4C=C(O)C(O)=C(O)C=4)C(O)=C(O)C=3)[C@@H](OC(=O)C=3C=C(OC(=O)C=4C=C(O)C(O)=C(O)C=4)C(O)=C(O)C=3)[C@H](OC(=O)C=3C=C(OC(=O)C=4C=C(O)C(O)=C(O)C=4)C(O)=C(O)C=3)O2)OC(=O)C=2C=C(OC(=O)C=3C=C(O)C(O)=C(O)C=3)C(O)=C(O)C=2)O)=C1 LRBQNJMCXXYXIU-PPKXGCFTSA-N 0.000 description 1
- 235000012874 Phaseolus calcaratus Nutrition 0.000 description 1
- 235000010627 Phaseolus vulgaris Nutrition 0.000 description 1
- 244000046052 Phaseolus vulgaris Species 0.000 description 1
- 235000006040 Prunus persica var persica Nutrition 0.000 description 1
- 235000010580 Psophocarpus tetragonolobus Nutrition 0.000 description 1
- 206010037660 Pyrexia Diseases 0.000 description 1
- 241000270295 Serpentes Species 0.000 description 1
- 206010040943 Skin Ulcer Diseases 0.000 description 1
- 208000004078 Snake Bites Diseases 0.000 description 1
- 229920002472 Starch Polymers 0.000 description 1
- 206010042674 Swelling Diseases 0.000 description 1
- 244000269722 Thea sinensis Species 0.000 description 1
- 208000007536 Thrombosis Diseases 0.000 description 1
- 241000046198 Triteleia hyacinthina Species 0.000 description 1
- 206010047513 Vision blurred Diseases 0.000 description 1
- 206010047700 Vomiting Diseases 0.000 description 1
- 239000004480 active ingredient Substances 0.000 description 1
- 230000006838 adverse reaction Effects 0.000 description 1
- 208000026935 allergic disease Diseases 0.000 description 1
- 230000007815 allergy Effects 0.000 description 1
- BJEPYKJPYRNKOW-UHFFFAOYSA-N alpha-hydroxysuccinic acid Natural products OC(=O)C(O)CC(O)=O BJEPYKJPYRNKOW-UHFFFAOYSA-N 0.000 description 1
- 230000036528 appetite Effects 0.000 description 1
- 235000019789 appetite Nutrition 0.000 description 1
- ODKSFYDXXFIFQN-UHFFFAOYSA-N arginine Natural products OC(=O)C(N)CCCNC(N)=N ODKSFYDXXFIFQN-UHFFFAOYSA-N 0.000 description 1
- 208000011775 arteriosclerosis disease Diseases 0.000 description 1
- 230000004888 barrier function Effects 0.000 description 1
- 229960003237 betaine Drugs 0.000 description 1
- 230000008827 biological function Effects 0.000 description 1
- 230000033228 biological regulation Effects 0.000 description 1
- 230000000740 bleeding effect Effects 0.000 description 1
- 230000017531 blood circulation Effects 0.000 description 1
- 230000036770 blood supply Effects 0.000 description 1
- 210000001124 body fluid Anatomy 0.000 description 1
- 239000010839 body fluid Substances 0.000 description 1
- 210000001185 bone marrow Anatomy 0.000 description 1
- 201000011510 cancer Diseases 0.000 description 1
- 230000010261 cell growth Effects 0.000 description 1
- 230000007969 cellular immunity Effects 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 235000012000 cholesterol Nutrition 0.000 description 1
- 208000019902 chronic diarrheal disease Diseases 0.000 description 1
- 235000017803 cinnamon Nutrition 0.000 description 1
- 230000004087 circulation Effects 0.000 description 1
- 230000006378 damage Effects 0.000 description 1
- 230000001934 delay Effects 0.000 description 1
- 230000029087 digestion Effects 0.000 description 1
- 230000001079 digestive effect Effects 0.000 description 1
- 238000004090 dissolution Methods 0.000 description 1
- 208000002173 dizziness Diseases 0.000 description 1
- 201000006549 dyspepsia Diseases 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- PEYUIKBAABKQKQ-UHFFFAOYSA-N epiasarinin Natural products C1=C2OCOC2=CC(C2OCC3C2COC3C2=CC=C3OCOC3=C2)=C1 PEYUIKBAABKQKQ-UHFFFAOYSA-N 0.000 description 1
- 230000004438 eyesight Effects 0.000 description 1
- 208000010706 fatty liver disease Diseases 0.000 description 1
- 235000008434 ginseng Nutrition 0.000 description 1
- 239000001947 glycyrrhiza glabra rhizome/root Substances 0.000 description 1
- 230000002710 gonadal effect Effects 0.000 description 1
- 230000003394 haemopoietic effect Effects 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 230000011132 hemopoiesis Effects 0.000 description 1
- HNDVDQJCIGZPNO-UHFFFAOYSA-N histidine Natural products OC(=O)C(N)CC1=CN=CN1 HNDVDQJCIGZPNO-UHFFFAOYSA-N 0.000 description 1
- 230000003054 hormonal effect Effects 0.000 description 1
- 229940088597 hormone Drugs 0.000 description 1
- 239000005556 hormone Substances 0.000 description 1
- 230000004727 humoral immunity Effects 0.000 description 1
- 230000036737 immune function Effects 0.000 description 1
- 210000000987 immune system Anatomy 0.000 description 1
- 230000001976 improved effect Effects 0.000 description 1
- 238000001727 in vivo Methods 0.000 description 1
- 230000001939 inductive effect Effects 0.000 description 1
- 230000004054 inflammatory process Effects 0.000 description 1
- 210000004347 intestinal mucosa Anatomy 0.000 description 1
- QXJSBBXBKPUZAA-UHFFFAOYSA-N isooleic acid Natural products CCCCCCCC=CCCCCCCCCC(O)=O QXJSBBXBKPUZAA-UHFFFAOYSA-N 0.000 description 1
- 210000003127 knee Anatomy 0.000 description 1
- 150000002632 lipids Chemical class 0.000 description 1
- 210000004072 lung Anatomy 0.000 description 1
- 239000002075 main ingredient Substances 0.000 description 1
- 239000001630 malic acid Substances 0.000 description 1
- 235000011090 malic acid Nutrition 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 230000006993 memory improvement Effects 0.000 description 1
- 230000027939 micturition Effects 0.000 description 1
- 230000007935 neutral effect Effects 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 235000020824 obesity Nutrition 0.000 description 1
- ZQPPMHVWECSIRJ-KTKRTIGZSA-N oleic acid Chemical compound CCCCCCCC\C=C/CCCCCCCC(O)=O ZQPPMHVWECSIRJ-KTKRTIGZSA-N 0.000 description 1
- 150000007524 organic acids Chemical class 0.000 description 1
- 230000000144 pharmacologic effect Effects 0.000 description 1
- 230000035790 physiological processes and functions Effects 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 238000010791 quenching Methods 0.000 description 1
- 230000005855 radiation Effects 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 230000001172 regenerating effect Effects 0.000 description 1
- 230000002040 relaxant effect Effects 0.000 description 1
- 230000003248 secreting effect Effects 0.000 description 1
- 210000000582 semen Anatomy 0.000 description 1
- VRMHCMWQHAXTOR-CMOCDZPBSA-N sesamin Natural products C1=C2OCOC2=CC([C@@H]2OC[C@@]3(C)[C@H](C=4C=C5OCOC5=CC=4)OC[C@]32C)=C1 VRMHCMWQHAXTOR-CMOCDZPBSA-N 0.000 description 1
- 231100000019 skin ulcer Toxicity 0.000 description 1
- 230000003860 sleep quality Effects 0.000 description 1
- 235000019698 starch Nutrition 0.000 description 1
- 239000008107 starch Substances 0.000 description 1
- 231100000240 steatosis hepatitis Toxicity 0.000 description 1
- 230000004936 stimulating effect Effects 0.000 description 1
- 239000013589 supplement Substances 0.000 description 1
- 229920002258 tannic acid Polymers 0.000 description 1
- LRBQNJMCXXYXIU-NRMVVENXSA-N tannic acid Chemical compound OC1=C(O)C(O)=CC(C(=O)OC=2C(=C(O)C=C(C=2)C(=O)OC[C@@H]2[C@H]([C@H](OC(=O)C=3C=C(OC(=O)C=4C=C(O)C(O)=C(O)C=4)C(O)=C(O)C=3)[C@@H](OC(=O)C=3C=C(OC(=O)C=4C=C(O)C(O)=C(O)C=4)C(O)=C(O)C=3)[C@@H](OC(=O)C=3C=C(OC(=O)C=4C=C(O)C(O)=C(O)C=4)C(O)=C(O)C=3)O2)OC(=O)C=2C=C(OC(=O)C=3C=C(O)C(O)=C(O)C=3)C(O)=C(O)C=2)O)=C1 LRBQNJMCXXYXIU-NRMVVENXSA-N 0.000 description 1
- 229940033123 tannic acid Drugs 0.000 description 1
- 235000015523 tannic acid Nutrition 0.000 description 1
- 238000002560 therapeutic procedure Methods 0.000 description 1
- 230000035922 thirst Effects 0.000 description 1
- 231100000167 toxic agent Toxicity 0.000 description 1
- 239000003440 toxic substance Substances 0.000 description 1
- 230000008736 traumatic injury Effects 0.000 description 1
- 150000003648 triterpenes Chemical class 0.000 description 1
- 210000002700 urine Anatomy 0.000 description 1
- 235000013311 vegetables Nutrition 0.000 description 1
- 230000009978 visual deterioration Effects 0.000 description 1
- 230000008673 vomiting Effects 0.000 description 1
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/06—Preparation of peptides or proteins produced by the hydrolysis of a peptide bond, e.g. hydrolysate products
Abstract
The invention discloses a preparation method and a process flow of small molecule compound peptide, belonging to the field of preparation of small molecule compound peptide, wherein the small molecule compound peptide can be prepared into powder or liquid preparation for oral administration, and can also be applied to specific functional aspects by combining with a formula; in the aspect of bioavailability, the small molecular compound peptide is many times of the absorption and utilization of common bovine bone collagen and sea cucumber protein; in the aspect of antioxidant effect, the small molecule compound peptide can improve the activity of superoxide dismutase and glutathione peroxidase, inhibit lipid peroxidation, remove hydroxyl free radical, reduce tissue oxidation and protect organisms.
Description
Technical Field
The invention relates to the field of preparation of small molecule compound peptides, in particular to a preparation method and a process flow of small molecule compound peptides.
Background
Peptides are small molecular substances indispensable for the connection of proteins and amino acids, and have a large number of functions and efficacies in the human body and are indispensable tissue components of the human body. The peptide has certain regulating function on nutrition and immunity of human body, improves human body constitution, and increases immune barrier, cellular immunity and humoral immunity of human body. In addition, peptides can regulate cholesterol metabolism in humans, for example, when cholesterol deficiency or peptide deficiency occurs in humans, cholesterol metabolism in the body is disturbed. The peptides are also capable of regulating the hormonal and endocrine secretory status of the human body. If the peptide is lacking in the body, endocrine imbalance and endocrine dyscrasia occur.
The preparation of small molecule peptide is usually carried out from animals and plants, most of the existing methods for preparing small molecule peptide are complex to operate, and the activity of the finished product of small molecule peptide is poor.
Disclosure of Invention
The invention provides a preparation method and a process flow of a small molecule compound peptide for solving the problems.
In order to achieve the purpose, the invention adopts the following technical scheme:
a preparation method and a process flow of small molecule compound peptide comprise the following steps:
s1, washing and crushing: according to the weight ratio of 90: 5: 5, weighing fresh ox bones, donkey-hide gelatin and fresh sea cucumbers according to the proportion, respectively soaking the fresh ox bones, the donkey-hide gelatin and the fresh sea cucumbers in warm water at the temperature of 80-90 ℃, cleaning and decontaminating, and respectively cutting the fresh ox bones, the donkey-hide gelatin and the fresh sea cucumbers into small pieces of 3-4 cm;
s2, high-pressure cooking: boiling fresh ox bones, donkey-hide gelatin and fresh sea cucumbers cut into small pieces in S1 at constant temperature and constant pressure for 8-12 hours at the temperature of 125-135 ℃ and the pressure of 0.15-0.20 MPa, and discharging residues to obtain mixed soup;
s3, magnetization: putting the mixed soup obtained in the step S2 into a strong magnetizer, and magnetizing for 8-10 seconds under a 4000-4800 gauss magnetic field;
s4, enzymolysis: heating the magnetized mixed soup in the S3 to 100-150 ℃, sterilizing, cooling to 45-55 ℃, adjusting the pH to 7.5-8.5, adding trypsin accounting for 0.8-1.2% of the weight of fresh ox bone, donkey-hide gelatin and fresh sea cucumber, and performing first enzymolysis for 2.5-3 hours; then adjusting the pH value to 6.0-6.5, and then adding bone-specific enzyme accounting for 0.3-0.4% of the weight of the fresh ox bone, the donkey-hide gelatin and the fresh sea cucumber, wherein the enzymolysis time is 3-4 hours; adjusting the pH value to 3.0-4.0, adding pepsin which is 0.6-1.0% of the weight of fresh ox bone, donkey-hide gelatin and fresh sea cucumber to carry out third enzymolysis to obtain an enzymolysis liquid;
s5, centrifugal separation: separating the enzymolysis liquid obtained in the step S4 by a high-speed centrifuge to obtain digestion-free small-molecule compound peptide, compound amino acid solution and other water-soluble nutrient components of the raw materials;
s6, sterilizing and inactivating the digestion-free small-molecule compound peptide and compound amino acid solution obtained by separation in S5 and other water-soluble nutrient components of the raw materials, keeping the temperature of the feed liquid at 80-90 ℃ for 15-25 seconds by using a sterilizer, and obtaining a sterilized and inactivated concentrated solution;
s7, concentrating the concentrated solution obtained after sterilization and enzyme deactivation in the S6 into more than 40% by a double-effect evaporator to obtain a small molecule compound peptide mixed solution product;
s8, hermetically packaging the small molecule compound peptide mixed liquid product obtained in S7, or pre-freezing the small molecule compound peptide mixed liquid product in S7 at the temperature of-40 ℃, then carrying out decompression freeze drying at the temperature of 1-10 pa to-40-60 ℃, preparing a small molecule compound peptide powder product, and hermetically packaging.
Preferably, the rotation speed value during the separation of the high-speed centrifuge in the step S5 is set within a range of 16000 to 18000 r/min.
Preferably, the S4 is stirred for 2-3 minutes every 1 hour in the enzymolysis process, so that the enzymolysis is uniformly and fully carried out.
Preferably, the pH of the S4 is adjusted by using a 10% NaOH solution or by using a 10% HC1 solution.
Preferably, the bone specific enzyme in S4 is one or more of dicer, exonuclease and flavor enzyme, and the activity of the bone specific enzyme is 500000 u/g.
Preferably, the trypsin activity in S4 is 2500000 u/g.
Preferably, the pepsin activity in S4 is 500000 u/g.
Preferably, the molecular weight of the small molecule composite peptide mixed solution product in S7 is 600-1400Da, the peptide segment is distributed in 300-2800Da, and is mainly distributed in 300-1100 Da.
Compared with the prior art, the invention provides a preparation method and a process flow of small molecule composite peptide, which has the following beneficial effects:
the invention has the beneficial effects that: the small molecule compound peptide can be prepared into powder or liquid preparation for oral administration, can be combined with a formula to be applied to specific functional aspects, and has good solubility performance and high dissolution rate even in cold water; in the aspect of bioavailability, the small molecular compound peptide is many times of the absorption and utilization of common bovine bone collagen and sea cucumber protein; in the aspect of antioxidant effect, the small molecule compound peptide can improve the activity of superoxide dismutase and glutathione peroxidase, inhibit lipid peroxidation, remove hydroxyl free radical, reduce tissue oxidation and protect organisms.
Drawings
Fig. 1 is a preparation flow chart of a specific embodiment of a preparation method and a process flow of a small molecule composite peptide provided by the invention.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments.
In the description of the present invention, it is to be understood that the terms "upper", "lower", "front", "rear", "left", "right", "top", "bottom", "inner", "outer", and the like, indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, are merely for convenience in describing the present invention and simplifying the description, and do not indicate or imply that the device or element being referred to must have a particular orientation, be constructed and operated in a particular orientation, and thus, should not be construed as limiting the present invention.
Example 1:
a preparation method and a process flow of small molecule compound peptide comprise the following steps:
s1, washing and crushing: according to the weight ratio of 90: 5: 5, weighing fresh ox bones, donkey-hide gelatin and fresh sea cucumbers according to the proportion, respectively soaking the fresh ox bones, the donkey-hide gelatin and the fresh sea cucumbers in warm water at the temperature of 80-90 ℃, cleaning and decontaminating, and respectively cutting the fresh ox bones, the donkey-hide gelatin and the fresh sea cucumbers into small pieces of 3-4 cm;
s2, high-pressure cooking: boiling fresh ox bones, donkey-hide gelatin and fresh sea cucumbers cut into small pieces in S1 at constant temperature and constant pressure for 8-12 hours at the temperature of 125-135 ℃ and the pressure of 0.15-0.20 MPa, and discharging residues to obtain mixed soup;
s3, magnetization: putting the mixed soup obtained in the step S2 into a strong magnetizer, and magnetizing for 8-10 seconds under a 4000-4800 gauss magnetic field;
s4, enzymolysis: heating the magnetized mixed soup in the S3 to 100-150 ℃, sterilizing, cooling to 45-55 ℃, adjusting the pH to 7.5-8.5, adding trypsin accounting for 0.8-1.2% of the weight of fresh ox bone, donkey-hide gelatin and fresh sea cucumber, and performing first enzymolysis for 2.5-3 hours; then adjusting the pH value to 6.0-6.5, and then adding bone-specific enzyme accounting for 0.3-0.4% of the weight of the fresh ox bone, the donkey-hide gelatin and the fresh sea cucumber, wherein the enzymolysis time is 3-4 hours; adjusting the pH value to 3.0-4.0, adding pepsin which is 0.6-1.0% of the weight of fresh ox bone, donkey-hide gelatin and fresh sea cucumber to carry out third enzymolysis to obtain an enzymolysis liquid;
s5, centrifugal separation: separating the enzymolysis liquid obtained in the step S4 by a high-speed centrifuge to obtain digestion-free small-molecule compound peptide, compound amino acid solution and other water-soluble nutrient components of the raw materials;
s6, sterilizing and inactivating the digestion-free small-molecule compound peptide and compound amino acid solution obtained by separation in S5 and other water-soluble nutrient components of the raw materials, keeping the temperature of the feed liquid at 80-90 ℃ for 15-25 seconds by using a sterilizer, and obtaining a sterilized and inactivated concentrated solution;
s7, concentrating the concentrated solution obtained after sterilization and enzyme deactivation in the S6 into more than 40% by a double-effect evaporator to obtain a small molecule compound peptide mixed solution product;
s8, hermetically packaging the small molecule compound peptide mixed liquid product obtained in S7, or pre-freezing the small molecule compound peptide mixed liquid product in S7 at the temperature of-40 ℃, then carrying out decompression freeze drying at the temperature of 1-10 pa to-40-60 ℃, preparing a small molecule compound peptide powder product, and hermetically packaging.
Further, it is preferable that the rotation speed value at the time of the high-speed centrifuge separation in S5 is set to range from 16000 to 18000 r/min.
Further, preferably, the S4 enzymolysis process is performed by stirring for 2-3 minutes every 1 hour, so that the enzymolysis is uniformly and sufficiently performed.
Further, it is preferable that the pH of the S4 is adjusted by using a 10% NaOH solution or a 10% HC1 solution.
Further, preferably, the bone specific enzyme in S4 is one or more of dicer, exonuclease and flavor enzyme, and the activity of the bone specific enzyme is 500000 u/g.
Further, preferably, the activity of trypsin in S4 is 2500000 u/g.
Further, preferably, the pepsin activity in S4 is 500000 u/g.
Further, preferably, the molecular weight of the small molecule composite peptide mixed solution product in S7 is 600-1400Da, the peptide segment is distributed in 300-2800Da, and mainly distributed in 300-1100 Da.
Example 2:
mixing and packaging the traditional Chinese medicine materials and the small molecule compound peptide mixed liquid product according to the following proportion: 10-40 parts of small-molecule compound peptide mixed liquor product, 10-12 parts of black sesame, 5-10 parts of wolfberry fruit, 10-12 parts of mulberry, 1-5 parts of cinnamon, 1-10 parts of dried orange peel, 5-10 parts of peach kernel, 5-10 parts of chicken's gizzard-membrane, 5-10 parts of sweet almond, 5-10 parts of fingered citron, 1-5 parts of mint, 1-5 parts of fructus amomi and 5-10 parts of honey-fried licorice root, and extracting the mixture into crude medicinal material liquid after mixing; and mixing the small-molecule composite peptide mixed solution with the extracted medicinal material raw solution, and decocting at the constant temperature of 60-80 ℃ for 3-5 hours to fully fuse the active peptide and the medicinal liquid for use.
Due to poor living and living, heavy working pressure and increasingly severe natural environment of modern people, a plurality of people are in sub-health state, and the product is designed for relieving the sub-health constitution of modern people; the application and development of modern science and technology and biotechnology provide strong scientific and technological support for the purification and utilization of effective ingredients of the finished product, and the traditional process flow is improved to a brand-new height, so that the efficacy of the effective ingredients of the product is guaranteed;
the product has effects in improving immunity, regulating blood sugar metabolism, lowering blood sugar, reducing blood lipid, resisting oxidation (aging), resisting allergy, resisting radiation, loosening bowel to relieve constipation, promoting digestion, improving memory, improving sleep quality, nourishing yin, replenishing blood, improving anemia, and resisting bacteria and virus;
the main component of the product is small molecular peptide which can be completely absorbed through small intestinal mucosa quickly and directly enter cells for conversion and utilization. Can regulate physiological functions of various systems and cells of a human body, maintain normal activities of in vivo hormones, digestive cell growth, circulation, nerves, reproduction and other systems, is also an important nutrient component required by growth and development of the human body, has a special biological function, can prevent and treat thrombus, hypertension and hyperlipidemia, resists fatigue, bacteria and viruses, delays aging, stimulates youth activity, and improves the immunity of the human body;
donkey-hide gelatin is neutral in nature and sweet in taste, and contains various amino acids such as lysine, arginine, histidine and the like as main ingredients. Has effects in nourishing yin, moistening dryness, replenishing blood, and stopping bleeding. Modern pharmacology proves that the donkey-hide gelatin also has the effects of enhancing the hematopoietic function of bone marrow, promoting the generation of hemoglobin and platelets, improving the internal environment of a human body, improving the immune function of the human body and the like; the donkey-hide gelatin peptide has the effects of improving immunity and tonifying qi and blood, the donkey-hide gelatin is a good substance for tonifying qi and blood, the sub-health of many people is also caused by mild anemia, and the donkey-hide gelatin peptide can be absorbed by a hundred percent of human bodies and is more easily accepted by the public compared with the donkey-hide gelatin;
sea cucumber peptide, sea cucumber, is not only a precious food, but also a rare medicinal material. Sea cucumber is known as Hai Wei Ge. Sea cucumber has the effects of tonifying kidney, replenishing vital essence, nourishing yin, tonifying yang and tonifying yin and yang. The sea cucumber has the effects of improving memory, delaying gonadal aging, preventing arteriosclerosis and diabetes, resisting tumors and the like. The sea cucumber has strong repairing and regenerating functions, can quickly heal wounds, repair damaged intestines and stomachs for many years, repair an immune system, repair islets, restore hematopoiesis, promote growth and development and the like;
wolfberry fruit, the traditional Chinese medicine of wolfberry fruit thinks: has effects in nourishing liver and kidney, improving eyesight, and moistening lung. Can be used for treating deficiency of liver-yin and kidney-yin, dizziness, blurred vision, visual deterioration, soreness of waist and knees, nocturnal emission, diabetes, asthenia, and cough. Modern pharmacology indicates that the active ingredients of the health-care food comprise polysaccharide, betaine, organic acid and the like, and the health-care food has the effects of enhancing human immunity, relieving physical fatigue, resisting oxidation, preventing aging and the like;
mulberry, mulberry, is sweet in taste and cold in nature, has the efficacies of nourishing yin and enriching blood, promoting the production of body fluid to quench thirst and moistening the intestines. Polysaccharides, tannic acid, malic acid and the like in the mulberry have the effects of delaying aging, promoting immunity, improving skin blood supply and the like;
poria cocos, has the effects of facilitating water and dampness removal, invigorating spleen, soothing the nerves and the like. Modern pharmacy shows that the polysaccharide, the triterpene and the compound thereof in the tuckahoe have the effects of reducing blood sugar, blood fat, memory improvement and the like;
the black sesame and the black sesame have the effects of tonifying liver and kidney, benefiting strength, moistening intestine and dryness, and replenishing essence and marrow. In ancient health preserving science of China, black sesame has been historically regarded as an important life-prolonging food. The effective components of the black sesame, such as oleic acid, linoleic acid, sesamin and the like, have the effects of reducing blood sugar and blood fat and relaxing bowel.
Example 3:
pre-freezing the mixed liquid product of the small molecule compound peptide at the temperature of minus 40 ℃, then carrying out decompression freeze drying at the temperature of 1-10 pa and minus 40-minus 60 ℃ to obtain the small molecule compound peptide freeze-dried powder, and breaking the walls of the small molecule compound peptide freeze-dried powder into powder through homology of medicine and food to mix the powder to prepare meal replacement powder, wherein the small molecule compound peptide: the proportion of the wall-broken mixture is 1-4: 9-6;
wherein the wall-breaking mixture is: 15-25 parts of phaseolus calcaratus, 20-25 parts of coix seed, 20-25 parts of white hyacinth bean, 10-25 parts of Chinese yam and 15-25 parts of gordon euryale seed;
the product has the functions of strengthening spleen, stimulating appetite, removing dampness, losing weight and regulating immunity, is used as meal replacement powder, and has a remarkable regulating effect on the physical condition of people with obesity; the product can not replace medicines, but all adopts food materials of 'homology of medicine and food' specified by related national departments, so that a human body in a sub-health state is adjusted to a normal state as soon as possible, thereby avoiding adverse reactions brought to the human body by the medicine bias of pure medicines and realizing the food therapy effect;
the meal replacement powder can reduce the blood fat content of a human body, has a remarkable improvement effect on the conditions of fatty liver, hyperlipidemia and the like, has a certain regulation effect on the blood sugar concentration of the human body, can avoid the increase of the blood sugar concentration after eating, and is beneficial to the alleviation and improvement of diabetes;
red bean, also known as red bean, red bean and vermilion bean, is a mature seed of leguminous plant red bean, which is a half-year-old wound herbaceous plant, and is cultivated all over the country, and the pods are harvested in summer and autumn when mature, dried in the sun, and collected for later use. Seeds are eaten; when used as a medicine, has the effects of promoting blood circulation, enriching blood, strengthening spleen, removing dampness, inducing diuresis and relieving swelling;
coix seeds, which are grown in warm and humid ten-sided land and valley stream ditches, are more common below the elevation of 2000 m. The nutritional value is rich, the pharmacological effect is achieved, the effects of oxidation resistance, inflammation resistance, cancer resistance, tumor resistance, blood sugar reduction, pain relief and the like are achieved, and the nutritional health-care tea is well loved by consumers;
bai Dou is treasure, and its peel (testa lablab), flower and leaf can be used as raw materials. The testa Dolichoris has effects of invigorating spleen and eliminating dampness, and can be used for treating dysentery, diarrhea, loempe, edema, etc.; flos Dolichoris has effects of relieving summer-heat, eliminating dampness, regulating stomach and invigorating spleen, and can be used for treating summer-heat dampness due to summer injury, fever, diarrhea, dysentery, leucorrhea with red and white discharge, traumatic injury and swelling, etc.; the folium Dolichoris has effects of removing summer-heat, promoting diuresis, removing toxic substance and relieving swelling, and can be used for treating summer-heat, dampness, vomiting and diarrhea, skin ulcer, furuncle, toxic swelling, and snake and insect bite;
the tuber of the Chinese yam is rich in starch and can be used for vegetables and foods; the medicine can tonify the deficiency of spleen and stomach, and treat deficiency of vital energy, asthenia, dyspepsia, spermatorrhea, enuresis, innominate toxic swelling, etc.;
semen euryales is recorded in the book Shennong Ben Cao Jing (Shennong's herbal Jing), listed as the top grade, and named as Jitouzi (Chinese pharmacopoeia) of the calendar edition, is a dry mature seed kernel of 1-year-old hydrophytic herbal gorgon euryale belonging to Nymphaeaceae, has the effects of tonifying kidney and securing essence, tonifying spleen and stopping diarrhea, and clearing damp and stopping leukorrhagia, and is mainly used for treating nocturnal emission, spermatorrhea, enuresis, frequent micturition, spleen deficiency and chronic diarrhea, whitish and turbid urine and leukorrhagia. The ginseng in water and the longan in water are reputed as traditional Chinese medicinal materials and precious natural supplements;
the above description is only for the preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art should be considered to be within the technical scope of the present invention, and the technical solutions and the inventive concepts thereof according to the present invention should be equivalent or changed within the scope of the present invention.
Claims (8)
1. A preparation method and a process flow of small molecule compound peptide are characterized by comprising the following steps:
s1, washing and crushing: according to the weight ratio of 90: 5: 5, weighing fresh ox bones, donkey-hide gelatin and fresh sea cucumbers according to the proportion, respectively soaking the fresh ox bones, the donkey-hide gelatin and the fresh sea cucumbers in warm water at the temperature of 80-90 ℃, cleaning and decontaminating, and respectively cutting the fresh ox bones, the donkey-hide gelatin and the fresh sea cucumbers into small pieces of 3-4 cm;
s2, high-pressure cooking: boiling fresh ox bones, donkey-hide gelatin and fresh sea cucumbers cut into small pieces in S1 at constant temperature and constant pressure for 8-12 hours at the temperature of 125-135 ℃ and the pressure of 0.15-0.20 MPa, and discharging residues to obtain mixed soup;
s3, magnetization: putting the mixed soup obtained in the step S2 into a strong magnetizer, and magnetizing for 8-10 seconds under a 4000-4800 gauss magnetic field;
s4, enzymolysis: heating the magnetized mixed soup in the S3 to 100-150 ℃, sterilizing, cooling to 45-55 ℃, adjusting the pH to 7.5-8.5, adding trypsin accounting for 0.8-1.2% of the weight of fresh ox bone, donkey-hide gelatin and fresh sea cucumber, and performing first enzymolysis for 2.5-3 hours; then adjusting the pH value to 6.0-6.5, and then adding bone-specific enzyme accounting for 0.3-0.4% of the weight of the fresh ox bone, the donkey-hide gelatin and the fresh sea cucumber, wherein the enzymolysis time is 3-4 hours; adjusting the pH value to 3.0-4.0, adding pepsin which is 0.6-1.0% of the weight of fresh ox bone, donkey-hide gelatin and fresh sea cucumber to carry out third enzymolysis to obtain an enzymolysis liquid;
s5, centrifugal separation: separating the enzymolysis liquid obtained in the step S4 by a high-speed centrifuge to obtain digestion-free small-molecule compound peptide, compound amino acid solution and other water-soluble nutrient components of the raw materials;
s6, sterilizing and inactivating the digestion-free small-molecule compound peptide and compound amino acid solution obtained by separation in S5 and other water-soluble nutrient components of the raw materials, keeping the temperature of the feed liquid at 80-90 ℃ for 15-25 seconds by using a sterilizer, and obtaining a sterilized and inactivated concentrated solution;
s7, concentrating the concentrated solution obtained after sterilization and enzyme deactivation in the S6 into more than 40% by a double-effect evaporator to obtain a small molecule compound peptide mixed solution product;
s8, hermetically packaging the small molecule compound peptide mixed liquid product obtained in S7, or pre-freezing the small molecule compound peptide mixed liquid product in S7 at the temperature of-40 ℃, then carrying out decompression freeze drying at the temperature of 1-10 pa to-40-60 ℃, preparing a small molecule compound peptide powder product, and hermetically packaging.
2. The preparation method and process flow of small molecule composite peptide according to claim 1, wherein the preparation method comprises the following steps: and the set range of the rotating speed value in the S5 during the separation of the high-speed centrifugal machine is 16000-18000 r/min.
3. The preparation method and process flow of small molecule composite peptide according to claim 1, wherein the preparation method comprises the following steps: stirring every 1 hour for 2-3 minutes in the S4 enzymolysis process to ensure that the enzymolysis is uniformly and fully carried out.
4. The preparation method and process flow of small molecule composite peptide according to claim 1, wherein the preparation method comprises the following steps: the pH of the S4 is adjusted by using a 10% NaOH solution or a 10% HC1 solution.
5. The preparation method and process flow of small molecule composite peptide according to claim 1, wherein the preparation method comprises the following steps: the bone specific enzyme in the S4 adopts one or more of incision enzyme, excision enzyme and flavor enzyme, and the activity of the bone specific enzyme is 500000 u/g.
6. The preparation method and process flow of small molecule composite peptide according to claim 1, wherein the preparation method comprises the following steps: the trypsin activity in S4 was 2500000 u/g.
7. The preparation method and process flow of small molecule composite peptide according to claim 1, wherein the preparation method comprises the following steps: the pepsin activity in S4 was 500000 u/g.
8. The preparation method and process flow of small molecule composite peptide according to claim 1, wherein the preparation method comprises the following steps: the molecular weight of the small molecule composite peptide mixed liquid product in S7 is 600-1400Da, the peptide segment is distributed at 300-2800Da, and is mainly distributed at 300-1100 Da.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111030760.3A CN113718007A (en) | 2021-09-03 | 2021-09-03 | Preparation method and process flow of small molecule compound peptide |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111030760.3A CN113718007A (en) | 2021-09-03 | 2021-09-03 | Preparation method and process flow of small molecule compound peptide |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN113718007A true CN113718007A (en) | 2021-11-30 |
Family
ID=78681444
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202111030760.3A Pending CN113718007A (en) | 2021-09-03 | 2021-09-03 | Preparation method and process flow of small molecule compound peptide |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN113718007A (en) |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103689212A (en) * | 2013-12-27 | 2014-04-02 | 冯启训 | Method for extracting small molecule peptide from fresh animal bone |
| CN105018554A (en) * | 2015-07-30 | 2015-11-04 | 北京天肽生物科技有限公司 | Small molecule bovine bone collagen peptide and preparation method thereof |
| CN105821106A (en) * | 2016-03-30 | 2016-08-03 | 蔡庭守 | Method for preparing sea cucumber small molecular peptide |
| CN107904274A (en) * | 2017-12-29 | 2018-04-13 | 山东东阿东方阿胶股份有限公司 | A kind of preparation method of donkey-hide gelatin active peptide |
| CN111670997A (en) * | 2020-06-26 | 2020-09-18 | 润科生物工程（福建）有限公司 | Preparation method of immune-enhancing compound protein peptidase hydrolyzed liquid, immune-enhancing compound protein peptide beverage and preparation method thereof |
| CN112941134A (en) * | 2021-03-09 | 2021-06-11 | 南京浩明乳业有限责任公司 | Whole sheep small molecule peptide extraction process |
-
2021
- 2021-09-03 CN CN202111030760.3A patent/CN113718007A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103689212A (en) * | 2013-12-27 | 2014-04-02 | 冯启训 | Method for extracting small molecule peptide from fresh animal bone |
| CN105018554A (en) * | 2015-07-30 | 2015-11-04 | 北京天肽生物科技有限公司 | Small molecule bovine bone collagen peptide and preparation method thereof |
| CN105821106A (en) * | 2016-03-30 | 2016-08-03 | 蔡庭守 | Method for preparing sea cucumber small molecular peptide |
| CN107904274A (en) * | 2017-12-29 | 2018-04-13 | 山东东阿东方阿胶股份有限公司 | A kind of preparation method of donkey-hide gelatin active peptide |
| CN111670997A (en) * | 2020-06-26 | 2020-09-18 | 润科生物工程（福建）有限公司 | Preparation method of immune-enhancing compound protein peptidase hydrolyzed liquid, immune-enhancing compound protein peptide beverage and preparation method thereof |
| CN112941134A (en) * | 2021-03-09 | 2021-06-11 | 南京浩明乳业有限责任公司 | Whole sheep small molecule peptide extraction process |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN111670997A (en) | Preparation method of immune-enhancing compound protein peptidase hydrolyzed liquid, immune-enhancing compound protein peptide beverage and preparation method thereof | |
| CN105296584A (en) | Preparation method of marine biological health care peptide preparation | |
| CN106578801A (en) | Comprehensive enzyme powder with hypoglycemic effect and preparation method thereof | |
| CN103610035A (en) | Preparation method for sea cucumber nutritional oral liquid | |
| CN106551398A (en) | A kind of anti-aging cream taste and preparation method thereof | |
| CN111671020A (en) | Polygonatum sibiricum beverage and preparation method thereof | |
| KR102042798B1 (en) | A method for preparing functional pills comprising extracts of natural materials | |
| CN107312691A (en) | A kind of fresh kidney beans wine and preparation method thereof | |
| CN103610076A (en) | Preparation method for sea cucumber capsule | |
| CN113718007A (en) | Preparation method and process flow of small molecule compound peptide | |
| CN106579384A (en) | Comprehensive enzyme powder with lipolysis function and preparation method of comprehensive enzyme powder with lipolysis function | |
| CN112998267A (en) | Oyster polypeptide vinegar egg liquid and preparation method thereof | |
| CN104673572A (en) | Anti-aging wine with functions of beautifying and keeping youthfulness and method for preparing anti-aging wine with functions of beautifying and keeping youthfulness | |
| KR20210041387A (en) | A natural tea removing the effect of hangover contained the extract material in arrowroot blossoms and Plantago ovata | |
| CN110679935A (en) | Burdock and ginseng essence cream and preparation method thereof | |
| KR20090021410A (en) | Sweetish jujube medical tea and sweetish jujube medical water | |
| CN110393246A (en) | A kind of oyster drink production method facilitating men's health | |
| CN104031805A (en) | Harmonizing health wine drunk in autumn and preparation method thereof | |
| CN103610068A (en) | Preparation method for blood-fat-reducing sea cucumber oral liquid | |
| CN111165797A (en) | Nutritional energy diet suitable for comprehensive regulation of physical functions of astronauts | |
| CN106174011A (en) | A kind of nutrient formulation nano-particle preventing and treating diabetes and preparation processing method | |
| CN108783358B (en) | Papaya and crucian thick soup | |
| KR20040023122A (en) | a composition which is composed of Acanthopanax senticosus and its manufacturing methods | |
| CN106174461A (en) | A kind of nutrient formulation nano-particle preventing and treating Sheehan syndrome and preparation processing method | |
| CN117229868A (en) | Sea cucumber maca wine and brewing method thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20211130 |