CN113699021A

CN113699021A - Constant-temperature strain expanding culture separating tank

Info

Publication number: CN113699021A
Application number: CN202110871031.4A
Authority: CN
Inventors: 杨莹; 叶冬青; 管敬喜; 何雪梅; 盛金凤; 辛明; 唐雅园; 易萍; 零东宁; 王雪峰
Original assignee: Guangxi Zhuang Nationality Autonomous Region Academy of Agricultural Sciences
Current assignee: Guangxi Zhuang Nationality Autonomous Region Academy of Agricultural Sciences
Priority date: 2021-07-30
Filing date: 2021-07-30
Publication date: 2021-11-26

Abstract

The invention relates to the field of strain culture devices, and particularly discloses a strain constant-temperature expanding culture separating tank, which comprises: the main frame body with swing arm and stand, urceolus, the inner tube of cover in the urceolus, wash port, elevating gear, hot plate, conducting ring, seal assembly, brush subassembly, drive arrangement. According to the invention, the inner cylinder is rotatably arranged in the outer cylinder, the inner cylinder is provided with the drainage hole, and when the inner cylinder rotates and centrifuges, culture solution or water is drained from the drainage hole under the action of centrifugal force, so that the effects of separating and cleaning strains are realized; the wall of the inner cavity is provided with a plurality of convex heating plates which can not only supply heat to the strain culture environment, but also can stir the liquid in the inner cavity more easily, so that the liquid can form vortex more easily and can be centrifuged; high-pressure gas can be introduced into the inner cavity, and the drain hole is sealed through the sealing component, so that the oxygen content can be conveniently controlled, and the working condition of high-pressure sterilization can be provided.

Description

Constant-temperature strain expanding culture separating tank

Technical Field

The invention belongs to the field of strain culture devices, and particularly relates to a strain constant-temperature expanding culture separation tank.

Background

Lactic acid bacteria are a general name of bacteria which can utilize fermentable carbohydrates to generate a large amount of lactic acid, are widely distributed in the natural world, have rich species diversity, are mostly indispensable in human bodies and have important flora with important physiological functions, are widely present in intestinal tracts of human bodies, and have extremely high application value in important fields closely related to human life, such as industry, agriculture and animal husbandry, food, medicine and the like.

In the production and experiment processes, a large amount of pure lactic acid bacteria are usually needed, the existing lactic acid bacteria culture mode usually needs to culture part of lactic acid bacteria through a culture dish under the condition of constant temperature and constant oxygen, and then the lactic acid bacteria are separated through a centrifugal machine, the existing lactic acid bacteria culture device can only separate a few lactic acid bacteria once, and can not directly separate a large amount of lactic acid bacteria from the culture solution, so that time and labor are wasted; and the separated lactobacillus can not be directly cleaned to obtain pure lactobacillus; when in culture, oxygen is required to be injected into the culture environment of the lactic acid bacteria, and the ventilation quantity of the oxygen is strictly controlled, so that the oxygen quantity is difficult to control by the conventional culture device; the culture environment needs to be sterilized before culture, and the best mode is autoclaving, but the existing culture device is difficult to perform autoclaving operation.

The above background disclosure is only for the purpose of assisting understanding of the inventive concept and technical solutions of the present invention, and does not necessarily belong to the prior art of the present patent application, and should not be used for evaluating the novelty and inventive step of the present application in the case that there is no clear evidence that the above content is disclosed at the filing date of the present patent application.

Disclosure of Invention

The invention aims to provide a strain constant-temperature expanding culture separation tank, so that the defects that a large amount of lactic acid bacteria cannot be simultaneously cultured, separated, cleaned and controlled by the conventional lactic acid bacteria production device and the culture environment cannot be autoclaved are overcome.

In order to achieve the above object, the present invention provides a strain constant temperature propagation separation tank, comprising: the main frame body comprises an upright post, one end of the upright post is fixedly provided with a base, the other end of the upright post is rotatably connected with one end of a swing arm, and the other end of the swing arm can swing towards the base; the outer barrel comprises a first end and a second end which are opposite, an outer cavity is arranged in the outer barrel, the first end is connected with the base through a lifting device, a first through hole is formed in the first end, a second through hole is formed in the second end, and the first through hole and the second through hole are communicated with the outer cavity; the inner cylinder is rotatably arranged in the outer cavity and comprises a third end and a fourth end which are opposite, the third end penetrates through the first through hole and extends out of the first end, the fourth end penetrates through the second through hole and extends out of the second end, an inner cavity is arranged in the inner cylinder, a discharge hole is formed in the third end, a feed hole is formed in the fourth end, a plurality of heating plates protruding outwards are arranged on the cavity wall of the inner cavity, a conducting ring is sleeved on the periphery of the fourth end, and the conducting ring is electrically connected with the heating plates; a plurality of drain holes are formed in the periphery of the inner cylinder, the drain holes are positioned close to the fourth end, a closed water storage cavity is enclosed between the inner cylinder and the outer cavity, and the inner cavity and the water storage cavity are communicated through the drain holes; the sealing assembly comprises a sealing plate arranged in the water storage cavity and a first hydraulic cylinder arranged on the outer cylinder, and the movable end of the first hydraulic cylinder is connected with the sealing plate and can drive the sealing plate to move towards the outer wall of the inner cylinder and seal the water drainage hole; the electric brush assembly comprises an electric brush arm, one end of the electric brush arm is connected with the stand column, the other end of the electric brush arm extends to the fourth end and is provided with an electric brush, and the end part of the electric brush is abutted to the outer side of the conducting ring; drive arrangement, it includes the elevating platform, the elevating platform is located on the swing arm, be equipped with driving motor on the elevating platform, driving motor's output is connected with the one end of connecting axle, the other end of connecting axle to the feed inlet extends and is equipped with sealing flange, the elevating platform can drive sealing flange with the feed inlet is laminated each other, be equipped with breather pipe and relief valve on the sealing flange.

Preferably, in the above technical scheme, a guide ring is fixedly arranged on the upright post, and the outer cylinder is slidably sleeved in the guide ring.

Preferably, in the above technical solution, the lifting device includes three or more second hydraulic cylinders, the second hydraulic cylinders are fixedly disposed on the base, and the movable ends of the second hydraulic cylinders are connected to the first ends.

Preferably, in the above technical solution, the middle portion of the inner barrel is cylindrical, and the diameter of the inner barrel is gradually reduced and smoothly transited from the middle portion to the first end and the second end.

Preferably, in the above technical solution, the heating plates are long, the length direction of the heating plates is parallel to the rotation axis of the inner cylinder, and the intervals between the heating plates are equal.

Preferably, in the above technical scheme, one end of the inner cavity close to the third end is provided with a material blocking ring protruding towards the inner cavity, and the material blocking ring is arranged on the periphery of the discharge hole.

Preferably, in the above technical scheme, a chamfer is arranged on one side of the material blocking ring close to the center thereof.

Preferably, in the above technical scheme, the first end is provided with a drain pipe, the drain pipe is communicated with the water storage cavity, and the drain pipe is provided with an electromagnetic valve.

Preferably, in the above technical scheme, the driving device further includes a third hydraulic cylinder, the third hydraulic cylinder is fixedly disposed on one side of the swing arm, which is far away from the outer cylinder, a movable end of the third hydraulic cylinder is connected with the lifting platform, and the other end of the connecting shaft penetrates through the swing arm and extends towards the outer cylinder.

Preferably, in the above technical scheme, the discharge port is provided with a discharge cover which can be opened and closed.

Compared with the prior art, the invention has the following beneficial effects:

1. the strain constant-temperature expanding culture separation tank is provided with an inner cylinder and an outer cylinder, wherein the inner cylinder is rotatably arranged in the outer cylinder, a drainage hole is formed in the position, close to the top, of the inner cylinder, and when the inner cylinder rotates and centrifuges, culture solution or water can flow to the top of the inner cylinder under the action of centrifugal force and is drained from the drainage hole, so that the effects of separating and cleaning strains are achieved; the wall of the inner cavity is provided with a plurality of convex heating plates which can not only supply heat to the strain culture environment, but also can stir the liquid in the inner cavity more easily, so that the liquid can form vortex more easily and can be centrifuged; high-pressure gas can be introduced into the inner cavity, and the drain hole is sealed through the sealing component, so that the oxygen content can be conveniently controlled, and the working condition of high-pressure sterilization can be provided.

2. The middle part of the inner cylinder is cylindrical, and the diameter of the inner cylinder is gradually reduced from the middle part to the two ends, so that water and culture solution can flow into the water storage cavity along the inclined outer wall of the inner cylinder immediately after flowing out of the drain hole in the process of centrifugation or cleaning, and the problem of backflow is avoided.

3. The electric brush in the electric brush assembly is abutted to the outer side of the conductive ring, and when the inner cylinder drives the conductive ring to rotate, the electric brush can also carry out continuous power-on heating on the heating plates so as to keep the constant temperature condition in the cylinder.

4. According to the invention, the bottom of the inner cavity of the inner barrel is provided with the material blocking ring, one side of the material blocking ring close to the center is provided with the chamfer, when the inner barrel rotates, water can flow from the center of the bottom to the periphery, but the outer side surface of the material blocking ring can block solid lactic acid bacteria from flowing back to the center, so that the integral centrifugal effect of the device can be improved.

5. Before the inner cylinder is subjected to bacteria culture, the inner cylinder can be driven to rotate to a certain position by a driving motor, then a sealing plate is pressed on the outer side of the inner cylinder through a hydraulic cylinder on a sealing assembly, so that a drain hole is sealed, then a sealing flange is pressed on a feed inlet through a lifting platform, and the interior of a tank body is pressurized through a vent pipe on the sealing flange, so that the high-pressure sterilization effect is achieved.

6. The driving device is arranged on the swing arm of the main frame body, and when the material needs to be fed from the feeding hole to the inner cavity, the swing arm can be rotated to open the sealing flange from the feeding hole, so that the material is convenient to change.

7. The outer barrel is arranged on the base through the lifting device, and when materials need to be taken from the material taking port, the outer barrel can be integrally lifted through the lifting device, so that an operator can conveniently scrape the materials from the material taking port outwards.

8. The guide ring is arranged on the upright post, so that the outer cylinder can be fixed and guided, and the situation that the inner cylinder topples or has overlarge amplitude due to vibration in the rolling process is prevented.

Drawings

FIG. 1 is a structural diagram of a constant-temperature expanding culture separating tank for strains according to the invention.

FIG. 2 is a partial cutaway view of the inner and outer barrels.

Fig. 3 is a sectional view of the outer cylinder.

FIG. 4 is a cut-away view of the inner barrel.

Fig. 5 is a structural view of the driving device.

Description of the main reference numerals:

1-main frame, 11-upright column, 12-base, 13-swing arm, 14-guide ring;

2-outer cylinder, 21-first end, 22-second end, 23-outer cavity, 24-first through hole, 25-second through hole, 210-drain pipe, 211-electromagnetic valve;

3-lifting device, 31-second hydraulic cylinder;

4-inner cylinder, 41-third end, 42-fourth end, 43-inner cavity, 44-discharge port, 441-discharge cover, 45-feed port, 46-heating plate, 47-conductive ring, 48-drain hole, 49-stop ring, 491-chamfer;

5-water storage cavity;

6-seal assembly, 61-seal plate, 62-first hydraulic cylinder;

7-brush assembly, 71-brush arm, 72-brush;

8-driving device, 81-lifting table, 82-driving motor, 83-connecting shaft, 84-sealing flange, 85-vent pipe, 86-pressure release valve and 87-third hydraulic cylinder.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

In the description of the present invention, it should be noted that the terms "central", "longitudinal", "lateral", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "top", "bottom", "inner", "outer", etc. indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, and are only for convenience of description and simplicity of description, but do not indicate or imply that the device or element referred to must have a specific orientation, be constructed in a specific orientation, and be operated, and thus, should not be construed as limiting the present invention.

In the description of the present invention, the meaning of a plurality of means is one or more, the meaning of a plurality of means is two or more, and larger, smaller, larger, etc. are understood as excluding the number, and larger, smaller, inner, etc. are understood as including the number. The terms "first", "second" and "third", if any, are used for descriptive purposes only and for distinguishing between technical features and are not to be construed as indicating or implying relative importance or implying a number of indicated technical features or a precedence of indicated technical features.

In the description of the present invention, it should be noted that, unless otherwise explicitly specified or limited, the terms "mounted," "connected," and "disposed" are to be construed broadly, e.g., as meaning either a fixed connection, a removable connection, or an integral connection; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meanings of the above terms in the present invention can be understood in specific cases to those skilled in the art. The following describes an embodiment of the present invention based on its overall structure.

As shown in fig. 1 to 5, the strain isothermal amplification and separation tank in this embodiment includes: the main frame body 1, the upright post 11, the base 12, the swing arm 13, the guide ring 14, the outer cylinder 2, the first end 21, the second end 22, the outer cavity 23, the first through hole 24, the second through hole 25, the drain pipe 210, the electromagnetic valve 211, the lifting device 3, the second hydraulic cylinder 31, the inner cylinder 4, the third end 41, the fourth end 42, the inner cavity 43, the discharge hole 44, the discharge cover 441, the feed inlet 45, the heating plate 46, the conducting ring 47, the drain hole 48, the material stopping ring 49, the chamfer 491, the water storage cavity 5, the sealing component 6, the sealing plate 61, the first hydraulic cylinder 62, the electric brush component 7, the electric brush arm 71, the electric brush 72, the driving device 8, the elevating platform 81, the driving motor 82, the connecting shaft 83, the sealing flange 84, the breather pipe 85, the pressure release valve 86, and the third hydraulic cylinder 87.

The main frame body 1 includes: the device comprises a vertical column 11, a base 12 and a swing arm 13, wherein one end of the vertical column 11 and one end of the base 12 are welded with each other, the base 12 is fixed on the ground, the other end of the vertical column 11 is rotatably connected with one end of the swing arm 13, and the other end of the swing arm 13 can swing towards the upper part of the base 12 and can rotate to a limit position perpendicular to the vertical column 11; the middle part of stand 11 is welded with guide ring 14, and the ring body of guide ring 14 is located the top of base 12.

The outer barrel 2 comprises a first end 21 and a second end 22 which are opposite, an outer cavity 23 is arranged in the outer barrel 2, the outer barrel 2 is slidably sleeved in the guide ring 14, the first end 21 is installed on the base 12 through the lifting device 3, a first through hole 24 is arranged on the end surface of the first end 21, a second through hole 25 is arranged on the end surface of the second end 22, the first through hole 24 and the second through hole 25 are communicated with the outer cavity 23 at the same time, and the axes of the first through hole 24 and the second through hole 25 are overlapped; the lifting device 3 comprises four second hydraulic cylinders 31, fixed ends of the second hydraulic cylinders 31 are fixedly mounted on the base 12, movable ends of the second hydraulic cylinders 31 are connected with the first ends 21, and the connection positions of the movable ends and the first ends are uniformly distributed on the periphery of the first through holes 24.

The inner barrel 4 is sleeved in the outer cavity 23 and can rotate, the inner barrel 4 comprises a third end 41 and a fourth end 42 which are opposite, the third end 41 penetrates through the first through hole 24 and extends out of the first end 21, the fourth end 42 penetrates through the second through hole 25 and extends out of the second end 22, an inner cavity 43 is arranged inside the inner barrel 4, a discharge hole 44 is formed in the end face of the third end 41, a feed hole 45 is formed in the end face of the fourth end 42, the middle of the inner barrel 4 is cylindrical, the diameter of the inner barrel 4 is gradually reduced from the middle of the inner barrel to the first end 21 and the second end 22 and is in smooth transition, and a discharge cover 441 which can be opened and closed is installed at the opening part of the discharge hole 44 through screws.

A plurality of strip-shaped heating plates 46 are arranged on the cavity wall of the inner cavity 43, the edges of the heating plates 46 protrude outwards from the cavity wall of the inner cavity 43, a conducting ring 47 is sleeved on the periphery of the fourth end 42, and the conducting ring 47 is electrically connected with the heating plates 46; a plurality of water drainage holes 48 are uniformly formed in the periphery of the inner cylinder 4, the positions of the water drainage holes 48 are close to the fourth end 42, when the inner cylinder 4 is installed in the outer cylinder 2, a closed water storage cavity 5 can be enclosed between the inner cylinder 4 and the outer cavity 23, and the water drainage holes 48 enable the inner cavity 43 and the water storage cavity 5 to be communicated with each other; a material blocking ring 49 is arranged at one end of the inner cavity 43 close to the third end 41, the material blocking ring 49 protrudes into the inner cavity 43, the material blocking ring 49 surrounds the periphery of the discharge hole 44, a chamfer 491 is arranged on one side surface of the material blocking ring 49 close to the center of the material blocking ring, and the outer side surface of the material blocking ring is a vertical surface; a water discharge pipe 210 is installed at the first end 21 of the outer tub 2, the water discharge pipe 210 and the water storage chamber 5 communicate with each other, and an electromagnetic valve 211 is installed on the water discharge pipe 210.

The sealing assembly 6 comprises a sealing plate 61 and a first hydraulic cylinder 62, the shape of the sealing plate 61 is matched with the outer side surface of the inner cylinder 4, the sealing plate 61 is positioned in the water storage cavity 5, a rubber layer is arranged on one surface, facing the inner cylinder 4, of the sealing plate 61, the first hydraulic cylinder 62 is installed on the outer side of the outer cylinder 2, the movable end of the first hydraulic cylinder 62 extends into the water storage cavity 5 and is connected with the sealing plate 61, and the first hydraulic cylinder 62 can drive the sealing plate 61 to move towards the outer wall of the inner cylinder 4 and seal the water drainage hole 48.

The brush assembly 7 comprises a brush arm 71 and a brush 72, wherein one end of the brush arm 71 is connected with the upright post 11, the other end of the brush arm 71 extends to the fourth end 42 and is provided with the brush 72, and the end part of the brush 72 is elastically bent towards one side of the conductive ring 47 and is abutted against the outer side of the conductive ring 47; both the brush 72 and the conductive ring 47 are made of copper.

The driving device 8 comprises a lifting platform 81 and three hydraulic cylinders 87, the number of the third hydraulic cylinders 87 is four, the three hydraulic cylinders 87 are mounted on the swing arm 13 and located on one side far away from the outer cylinder 2, the movable ends of the four third hydraulic cylinders 87 are respectively connected with four corners of the lifting platform 81, a driving motor 82 is mounted on the lifting platform 81, the output end of the driving motor 82 is connected with one end of a connecting shaft 83, the other end of the connecting shaft 83 penetrates through the swing arm 13 and extends towards one side of the outer cylinder 2, when the swing arm 13 rotates to the upper side of the outer cylinder 2 and forms a 90-degree included angle with the upright post 11, the other end of the connecting shaft 83 is aligned with the feed port 45 and extends towards the feed port, a sealing flange 84 is arranged at the other end of the connecting shaft 83, the lifting platform 81 can drive the sealing flange 84 and the feed port 45 to be mutually attached and fastened through bolts, and a vent pipe 85 and a pressure release valve 86 are arranged on the sealing flange 84.

Next, the working principle of the strain constant-temperature propagation and separation tank in this embodiment will be described in detail to make those skilled in the art understand the present invention:

before use, the lifting platform 81 is lifted and the swing arm 13 is rotated to the side far away from the outer cylinder 2, the first hydraulic cylinder 62 of the sealing assembly 6 is started to enable the sealing plate 61 to cover the water discharge hole 48, then culture solution is added into the inner cylinder 4 from the feed port 45, then the swing arm 13 is swung to the upper part of the outer cylinder 2, the lifting platform 81 is put down, the sealing flange 84 is covered on the feed port 45 and is tightened and sealed through bolts, then gas is introduced into the inner cavity 43 through the vent pipe 85 to be pressurized, and pressurized sterilization is carried out.

When the device is used, lactobacillus strains are added into the inner cylinder 4 through the feeding hole 45, then the sealing flange 84 is covered, the electric brush assembly 7 is used for electrifying the conducting ring 47, the heating plate 46 is heated, the temperature is kept at about 40 ℃, and the strains are cultured; after the culture is finished, the sealing plate 61 of the sealing assembly 6 is opened, the inner cylinder is rotated through the driving device 8, so that the culture solution and the strains are separated, the strains are precipitated at the bottom of the inner cylinder 4 after centrifugation, the culture solution is thrown out of the water storage cavity 5 through the water drainage hole 48 above the strain, and is discharged through the water drainage pipe 210, and then water can be continuously added into the inner cylinder 2 to clean the strains and spin-dry the strains in a centrifugal mode.

After the expanding culture separation treatment, the lifting platform 81 on the driving device 8 is lifted, the sealing flange 84 is lifted, the swing arm 13 rotates to the other side, then the lifting device 3 lifts the whole outer cylinder 2, the discharging cover 411 is opened, and the cleaned and separated strains are taken out from the discharging hole 44.

In summary, the inner cylinder is rotatably arranged in the outer cylinder, the position of the inner cylinder close to the top is provided with the drainage hole, when the inner cylinder rotates and centrifuges, the culture solution or water can flow to the top of the inner cylinder under the action of centrifugal force and can be drained from the drainage hole, so that the effects of separating and cleaning strains are achieved; the wall of the inner cavity is provided with a plurality of convex heating plates which can not only supply heat to the strain culture environment, but also can stir the liquid in the inner cavity more easily, so that the liquid can form vortex more easily and can be centrifuged; high-pressure gas can be introduced into the inner cavity, and the drain hole is sealed through the sealing component, so that the oxygen content can be conveniently controlled, and the working condition of high-pressure sterilization can be provided.

The foregoing description of the specific exemplary embodiments of the invention has been presented for the purposes of illustration and description and is not intended to limit the invention to the precise form disclosed, and obviously many modifications and variations are possible in light of the above teaching, although examples of the invention are shown and described, the present examples are intended to be illustrative of the invention and are not to be considered limiting, and the particular features, structures, materials, or characteristics described may be suitably combined in any one or more of the examples or examples, selected from and described to explain certain principles of the invention and its practical application, to thereby enable others skilled in the art to modify, if necessary, the examples without materially departing from the principles and spirit of the invention, Alternatives, modifications, and variations are possible but are within the scope of the appended claims.

Claims

1. A strain constant-temperature expanding culture separating tank is characterized by comprising:

the main frame body (1) comprises a stand column (11), a base (12) is fixedly arranged at one end of the stand column (11), the other end of the stand column (11) is rotatably connected with one end of a swing arm (13), and the other end of the swing arm (13) can swing towards the base (12);

the outer barrel (2) comprises a first end (21) and a second end (22) which are opposite, an outer cavity (23) is arranged inside the outer barrel (2), the first end (21) is connected with the base (12) through a lifting device (3), a first through hole (24) is formed in the first end (21), a second through hole (25) is formed in the second end (22), and the first through hole (24) and the second through hole (25) are communicated with the outer cavity (23);

the inner cylinder (4) is rotatably arranged in the outer cavity (23), the inner cylinder (4) comprises a third end (41) and a fourth end (42) which are opposite, the third end (41) penetrates through the first through hole (24) and extends out of the first end (21), the fourth end (42) penetrates through the second through hole (25) and extends out of the second end (22), an inner cavity (43) is arranged inside the inner cylinder (4), the third end (41) is provided with a discharge hole (44), the fourth end (42) is provided with a feed inlet (45), a plurality of heating plates (46) which protrude outwards are arranged on the cavity wall of the inner cavity (43), a conductive ring (47) is sleeved on the periphery of the fourth end (42), and the conductive ring (47) is electrically connected with the heating plates (46); a plurality of drain holes (48) are formed in the periphery of the inner cylinder (4), the drain holes (48) are close to the fourth end (42), a closed water storage cavity (5) is defined between the inner cylinder (4) and the outer cavity (23), and the inner cavity (43) and the water storage cavity (5) are communicated through the drain holes (48);

the sealing assembly (6) comprises a sealing plate (61) arranged in the water storage cavity (5) and a first hydraulic cylinder (62) arranged on the outer cylinder (2), wherein the movable end of the first hydraulic cylinder (62) is connected with the sealing plate (61) and can drive the sealing plate (61) to move towards the outer wall of the inner cylinder (4) and seal the drain hole (48);

the electric brush assembly (7) comprises an electric brush arm (71), one end of the electric brush arm (71) is connected with the upright post (11), the other end of the electric brush arm (71) extends to the fourth end (42) and is provided with an electric brush (72), and the end part of the electric brush (72) is abutted with the outer side of the conductive ring (47);

drive arrangement (8), it includes elevating platform (81), elevating platform (81) are located on swing arm (13), be equipped with driving motor (82) on elevating platform (81), the output of driving motor (82) is connected with the one end of connecting axle (83), the other end of connecting axle (83) to feed inlet (45) extend and are equipped with sealing flange (84), elevating platform (81) can drive sealing flange (84) with feed inlet (45) laminate each other, be equipped with breather pipe (85) and relief valve (86) on sealing flange (84).

2. The strain constant-temperature expanded culture separation tank of claim 1, wherein a guide ring (14) is fixedly arranged on the upright column (11), and the outer cylinder (2) is slidably sleeved in the guide ring (14).

3. The strain constant-temperature expanding culture separating tank of claim 1, wherein the lifting device (3) comprises three or more second hydraulic cylinders (31), the second hydraulic cylinders (31) are fixedly arranged on the base (12), and the movable ends of the second hydraulic cylinders (31) are connected with the first ends (21).

4. The strain isothermal propagation knockout drum according to claim 1, characterized in that the middle of the inner drum (4) is cylindrical, and the diameter of the inner drum (4) is gradually reduced and smoothly transited from the middle to the first end (21) and the second end (22).

5. The strain constant-temperature expanding culture separating tank as claimed in claim 1, wherein the heating plates (46) are long strips, the length direction of the heating plates is parallel to the rotation axis of the inner cylinder (4), and the intervals between the heating plates (46) are equal.

6. The strain constant-temperature expanding culture separating tank of claim 1, wherein a material blocking ring (49) protruding towards the inner cavity (43) is arranged at one end of the inner cavity (43) close to the third end (41), and the material blocking ring (49) is annularly arranged on the periphery of the material outlet (44).

7. The strain constant-temperature expanding culture separating tank of claim 6, wherein one side of the baffle ring (49) close to the center thereof is provided with a chamfer (491).

8. The strain constant-temperature expanding culture separating tank of claim 1, wherein the first end (21) is provided with a drain pipe (210), the drain pipe (210) is communicated with the water storage cavity (5), and the drain pipe (210) is provided with an electromagnetic valve (211).

9. The strain constant-temperature expanding culture separating tank of claim 1, wherein the driving device (8) further comprises a third hydraulic cylinder (87), the third hydraulic cylinder (87) is fixedly arranged on one side of the swing arm (13) far away from the outer cylinder (2), the movable end of the third hydraulic cylinder (87) is connected with the lifting platform (81), and the other end of the connecting shaft (83) penetrates through the swing arm (13) and extends towards the outer cylinder (2).

10. The strain constant-temperature propagation separation tank as claimed in claim 9, wherein the discharge port (44) is provided with a discharge cover (441) which can be opened and closed.