CN110836808B - Intestinal excrement detection system - Google Patents

Intestinal excrement detection system Download PDF

Info

Publication number
CN110836808B
CN110836808B CN201911135718.0A CN201911135718A CN110836808B CN 110836808 B CN110836808 B CN 110836808B CN 201911135718 A CN201911135718 A CN 201911135718A CN 110836808 B CN110836808 B CN 110836808B
Authority
CN
China
Prior art keywords
liquid level
centrifugal
cavity
centrifugal cavity
sensor group
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
CN201911135718.0A
Other languages
Chinese (zh)
Other versions
CN110836808A (en
Inventor
张俊美
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Individual
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Priority to CN201911135718.0A priority Critical patent/CN110836808B/en
Publication of CN110836808A publication Critical patent/CN110836808A/en
Application granted granted Critical
Publication of CN110836808B publication Critical patent/CN110836808B/en
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Images

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/38—Diluting, dispersing or mixing samples
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/286—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q involving mechanical work, e.g. chopping, disintegrating, compacting, homogenising
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/34—Purifying; Cleaning

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

The invention provides a detection system of intestinal feces, and a detection method of the system comprises the following steps: step 1, adding physiological saline and excrement into a first centrifugal cavity; step 2, the stirring device of the first centrifugal cavity enters a stirring mode; step 3, the second liquid level sensor group starts to detect the liquid level height in the second centrifugal cavity; step 4, the filtrate enters a second centrifugal cavity for centrifugal treatment, and the primary centrifugal precipitate after the supernatant is removed enters a transfer cavity; step 5, the second centrifugal cavity enters emptying operation; step 6, returning the secondary centrifugal precipitate in the transfer cavity to the first centrifugal cavity for centrifugation; step 7, the transfer cavity enters into an emptying operation; step 8, opening the first control valve again, and allowing the third centrifugal precipitate to enter a second centrifugal cavity; the physiological saline in the output storage bin flows into a second centrifugal cavity, and the second centrifugal cavity performs centrifugal work; and 9, allowing the fecal bacteria suspension meeting the requirements in the second centrifugal cavity to enter a transfer cavity for storage.

Description

Intestinal excrement detection system
Technical Field
The invention relates to the technical field of fecal bacteria transplantation, in particular to a detection system for intestinal excrement.
Background
The 'fecal strain transplantation' is defined as transplanting functional flora in feces of healthy people into gastrointestinal tracts of patients to reconstruct new intestinal flora and realize the treatment of intestinal tract and parenteral diseases; fecal bacteria for fecal bacteria transplantation the fecal bacteria is derived from feces from healthy donors and the procedure involves: the method comprises the following main steps of donor screening, preparation before donor donation, feces collection, fecal strain separation and purification, fecal strain preservation, resuscitation and the like:
screening a donor:
to select qualified donors, we need to thoroughly screen donor volunteers to eliminate various infectious diseases, hereditary diseases, and various bacterial and viral infections, and to eliminate all possible factors affecting the intestinal flora, so as to select the most suitable donor flora. The main screening and exclusion indexes are as follows: 1. the history of taking the medicine, antibiotics, laxatives and weight-reducing medicines are used within three months, and immunosuppressants and chemotherapeutics are taken; 2. various known infectious diseases, malignant obesity, diabetes, inflammatory bowel disease, allergy, metabolic syndrome, hypoimmunity, irritable bowel syndrome, chronic diarrhea or constipation, chronic fatigue syndrome, gastrointestinal malignant tumor or polyp; 3. positive laboratory virology and pathogen tests, blood test: HIV virus and hepatitis A, B, C virus positive, syphilis antibody, stool examination or culture: difficile clostridial toxins, giardia lamblia, cryptosporidium, various parasites and eggs; 4. history of high risk behaviors, history of drug use or illicit drug use, recent history of prohibition or travel in an affected area.
II, preparing donors before donation:
step 1, preparing diet, wherein the structure of intestinal flora is easily influenced by diet, and in order to obtain healthy flora, the diet of a donor is adjusted one week before donation according to a preset diet reference table, so that high-sugar, high-fat and irritant diet is avoided, high-protein food such as seafood, fish and shrimp is reduced, and fruits and vegetables and food rich in dietary fiber are eaten more.
Step 2, a small amount of osmotic laxative was given 12 hours prior to treatment to aid donor bowel movement.
Thirdly, fecal strain preparation steps:
step 1, collecting fresh excrement and putting the fresh excrement into a special excrement collecting barrel;
step 2, installing the excrement collecting barrel into an automatic excrement and bacteria separating system, adding normal saline for dilution, and stirring for 10 minutes by a machine;
and 3, filtering and purifying: removing excrement residues and large-particle substances mainly through a multi-stage ultrafiltration system of the instrument, and collecting final filtrate;
step 4, centrifugal rinsing: repeatedly centrifuging to remove apical fluid, adding physiological saline for resuspension, and finally obtaining purified centrifugal precipitate;
step 5, preserving fecal bacteria: the purified fecal strain can be directly used for treating patients, and can also be added with fecal strain preserving fluid and preserved at-80 deg.C;
and 6, recovering fecal bacteria: taking out the fecal strain 1 hour before use, dissolving at room temperature, centrifuging, cleaning to remove redundant preservation solution, adding normal saline to suspend, and making into fecal strain suspension for treating patients.
In the prior art, all the operation steps are required to be completed in a special fecal bacteria preparation laboratory, and part of the work is required to be completed in a biological safety cabinet. All equipment needs to be disinfected, disposable consumables are aseptic consumables, and all operators need to wear gloves, masks and caps to avoid the contamination of the fecal bacteria by exogenous pathogenic bacteria; the whole operation process is complicated in form, and the sense of operators is influenced because the materials for preparing the fecal strain are mainly feces.
To this end, the present invention provides a detection system for intestinal stool that at least partially solves the above-mentioned problems.
Disclosure of Invention
In this summary, concepts in a simplified form are introduced that are further described in the detailed description section. This summary of the invention is not intended to identify key features or essential features of the claimed subject matter, nor is it intended to be used as an aid in determining the scope of the claimed subject matter.
In order to at least partially solve the technical problem, the invention provides a detection system for intestinal tract feces, and a detection method of the system comprises the following steps:
step 1, adding physiological saline and excrement filtrate into a first centrifugal cavity;
step 2, a stirring device of the first centrifugal cavity enters a stirring mode, a heating device in the first centrifugal cavity starts to work, the stirring device is used for mixing excrement filtrate and normal saline to dilute excrement, and the heating device is used for heating the normal saline and the excrement filtrate in the first centrifugal cavity to a specified temperature and maintaining the specified temperature;
when the stirring device in the first centrifugal cavity works for a first standard time, the stirring device stops working, the first control valve is opened, liquid in the first centrifugal cavity flows into the second centrifugal cavity after being filtered by the nanoscale filter screen, and residues and large granular substances in excrement filtrate stay in the first centrifugal cavity;
step 3, the second liquid level sensor group starts to detect the liquid level height in the second centrifugal cavity, when the second real-time liquid level height measured by the second liquid level sensor group meets a first liquid level standard value, the first control valve is closed, and at the moment, the first centrifugal cavity enters an emptying operation;
step 4, filtrate in the first centrifugal cavity enters a second centrifugal cavity, when a second real-time liquid level height measured by a second liquid level sensor group meets a first liquid level standard value, the second centrifugal cavity starts to perform centrifugal work, after a second standard time, the second control valve is opened, and primary centrifugal precipitate in the second centrifugal cavity enters a transfer cavity;
step 5, a third liquid level sensor group starts to detect the liquid level height in the transfer cavity, when the third real-time liquid level height detected by the third liquid level sensor group meets a second liquid level standard value, the second control valve is closed, and at the moment, the second centrifugal cavity enters an emptying operation to discharge supernatant liquid left in the cavity;
step 6, the first liquid level sensor group starts to detect the liquid level height in the first centrifugal cavity, when the first liquid level sensor group detects that the liquid level in the first centrifugal cavity is zero, the controller controls the stop valve to be opened, meanwhile, the controller also controls the conveying pump to work, and secondary centrifugal sediments in the transfer cavity return to the first centrifugal cavity under the action of the conveying pump;
step 7, the first liquid level sensor group continues to detect the liquid level height in the first centrifugal cavity, and when the first real-time liquid level height detected by the first liquid level sensor group meets a third liquid level standard value, the stop valve is closed;
step 8, when the third real-time liquid level height measured by the first liquid level sensor group meets a third liquid level standard value, the first centrifugal cavity starts to perform centrifugal work, after a second standard time, the first control valve is opened again, and the third centrifugal precipitate enters the second centrifugal cavity; the second liquid level sensor group detects the liquid level height in the second centrifugal cavity again, when the second real-time liquid level height detected by the second liquid level sensor group meets a fourth liquid level standard value, the first control valve is closed, the intermediate output device starts to work, the output control valve is opened, so that the normal saline in the output storage bin flows into the second centrifugal cavity, when the second real-time liquid level height detected by the second liquid level sensor group meets a fifth liquid level standard value, the intermediate output device stops working, the second centrifugal cavity performs centrifugal work again, and the heating device heats the liquid in the second centrifugal cavity to a specified temperature;
and 9, after the second centrifugal cavity enters a stirring mode to work for a third standard time, the second control valve is opened, liquid in the second centrifugal cavity flows into the transfer cavity, the third liquid level sensor group starts to detect the liquid level height in the transfer cavity, when the third real-time liquid level height detected by the third liquid level sensor group meets a sixth liquid level standard value, the second control valve is closed, and the fecal bacteria suspension meeting the requirements is stored in the transfer cavity.
Further, a system for detecting intestinal fecal material is also provided, the system comprising: a first centrifuge tank, a second centrifuge tank, the diversion pipeline, the first control valve, the intermediate output device, the front return pipeline, the rear return pipeline, and a transfer tank;
the first centrifugal cavity and the second centrifugal cavity are communicated through the diversion pipeline, and the horizontal height of the first centrifugal cavity is higher than that of the second centrifugal cavity; the nanoscale filter screen is arranged between the first control valve and the first centrifugal cavity and is used for filtering liquid flowing out of the first centrifugal cavity; the intermediate output device comprises the output storage bin, the output control valve and the output control pipeline; the output material storage bin is communicated with the diversion pipeline through the output control pipeline, the output control valve is arranged on the output control pipeline, a transfer cavity is arranged in the transfer tank, and the front return pipeline is communicated with the second centrifugal cavity and the transfer cavity; the rear return pipeline is communicated with the transfer cavity and the first centrifugal cavity; the second control valve is arranged near a joint of the front return pipeline and the second centrifugal cavity and used for controlling the connection and disconnection of the second centrifugal cavity and the front return pipeline; the rear backflow pipeline is provided with the conveying pump so as to convey the transferred material to the first centrifugal cavity; the connecting part of the first centrifugal cavity and the rear backflow pipeline is provided with the check valve, the check valve is opened along with the opening of the conveying pump, and the check valve is closed along with the closing of the conveying pump.
Further, the liquid level standard value is calculated in the following way:
the calculation method of the first liquid level standard value H1 comprises the following steps: h1 ═ QGeneral (1)- Δ 1, wherein QGeneral assemblyIn order to add the volume of the normal saline, delta 1 is the volume loss of the material flowing through the diversion pipeline;
the method for calculating the second liquid level standard value H2 comprises the following steps: h2 ═ QGeneral assembly- Δ 1- Δ 2- α, wherein Δ 2 is the volume loss of material flowing through the front reflux line, α is the volume of supernatant, and α is linear with the amount of fecal filtrate added;
the calculation method of the third liquid level standard value H3 comprises the following steps: h3 ═ H2- Δ 3-1.5 α, where Δ 3 is the loss of material through the post return line;
the calculation of the fourth level criterion value H4 includes: h4 ═ H3-2 α;
the calculation mode of the fifth liquid level standard value H5 comprises the following steps: h5 ═ H4- Δ 1-1.7 α;
the calculation of the sixth level criterion value H6 includes: h6 ═ H5- Δ 2+ kQGeneral assembly；
Wherein k is a fixed coefficient, the specific value of k is determined by the staff, kQGeneral assemblyThe volume of saline input to the second centrifugal chamber for the intermediate output device.
Further, the time criterion is calculated by:
the first standard time T1 is calculated by: t1 ═ 3+ QGeneral assemblyN, wherein n is a positive integer and T1 has units of minutes;
the second standard time T2 is calculated by: t2 ═ 2+ H5/n, units of T2 are minutes;
the third standard time T3 is calculated by: t3 ═ 2+ H6/n, and T3 units are minutes.
Furthermore, the bottom of the centrifugal tank is provided with a stirring device extending to the middle part of the centrifugal cavity, and the top of the centrifugal tank is provided with a test paper clamping device; and a heating device is arranged in a space enclosed between the outer surface of the centrifugal cavity and the inner wall of the centrifugal tank.
Furthermore, a first liquid inlet and outlet is formed in the outer side of the first centrifugal tank; a second liquid inlet and outlet is formed in the outer side of the second centrifugal tank; the transfer tank is provided with a third liquid inlet and outlet;
the emptying operation comprises a waste discharging stage in the cavity and a high-temperature sterilizing stage;
when the second cavity body enters an emptying operation, the liquid inlet and outlet is opened, residues in the cavity body flow out of the centrifugal cavity or the transfer cavity along the liquid inlet and outlet, and when the liquid level sensor group detects that the liquid level in the centrifugal cavity is stable, clear water is injected into the centrifugal cavity through the liquid inlet and outlet for cleaning; after clear water enters the centrifugal cavity, the liquid inlet and outlet are closed, the stirring device starts stirring, the stirring device stops working after stirring for a certain time, and the liquid inlet and outlet are opened again to discharge waste liquid; and after the stage of discharging wastes in the cavity is finished, the centrifugal cavity enters a high-temperature sterilization stage, wherein the centrifugal cavity is subjected to high-temperature sterilization by the heating device, and the centrifugal cavity is heated for three minutes in a high-temperature state by the heating device so as to achieve a sterilization effect.
The test paper clamping device comprises a first centrifugal tank, a second centrifugal tank and a middle tank, and is characterized by further comprising a camera, wherein the camera is arranged in an area enclosed by the first centrifugal tank, the second centrifugal tank and the middle tank, the camera is provided with three cameras, and the cameras respectively face the test paper clamping device of the first centrifugal tank, the second centrifugal tank and the middle tank, so that the cameras feed back data on test paper to a computer.
Further, the control system comprises a control group and a controlled element;
wherein, the control group includes controller and control panel, the controller electricity is connected control panel, the controller is controlled the component through the control and in order to realize control function, controlled the component includes first temperature sensor group, second temperature sensor group, third temperature sensor group, first velocity of flow sensor group, second velocity of flow sensor group, third velocity of flow sensor group first liquid level sensor group second liquid level sensor group third liquid level sensor group, lamps and lanterns controlling means, sealer, glue paper roll first control valve the delivery pump second control valve the restriction valve, the motor and the output thing control valve.
Further, the first temperature sensor group is arranged in the first centrifugal cavity to detect a first real-time temperature in the first centrifugal cavity; the second temperature sensor group is arranged in the second centrifugal cavity to detect a second real-time temperature in the second centrifugal cavity; the third temperature sensing group is arranged in the transfer cavity to detect a third real-time temperature in the transfer cavity; the first flow sensor group is arranged on the diversion pipeline and at the front end of the intermediate output device so as to detect the real-time flow velocity V1 of the material flowing out of the first centrifugal cavity; the second flow sensor group is arranged on the output control pipeline to detect the real-time flow rate V2 of the intermediate; the third flow rate sensor group is arranged on the rear return pipeline to detect the real-time flow rate V3 of the material flowing out of the transfer cavity; the first liquid level sensor group is arranged in the first centrifugal cavity to detect a first real-time liquid level height of the first centrifugal cavity; the second liquid level sensor group sets up in the centrifugal intracavity of second, in order to detect the real-time liquid level height of second in the centrifugal intracavity of second, third liquid level sensor group sets up in the transfer intracavity, in order to detect the real-time liquid level height of third in the transfer intracavity.
Further, the input state quantities of the controller include: total amount of saline added for the first time QGeneral assemblyCalculating a standard number n according to the precipitation volume alpha, the fixed coefficient k and the loss difference delta 4 time; and the input state quantity of the controller can be changed by a worker through the control panel.
Compared with the prior art, the invention has the beneficial effects that: the invention provides a detection system of intestinal feces and a detection method using the system, wherein the method comprises the following steps:
step 1, adding physiological saline and excrement filtrate into a first centrifugal cavity;
step 2, a stirring device of the first centrifugal cavity enters a stirring mode, a heating device in the first centrifugal cavity starts to work, the stirring device is used for mixing excrement filtrate and normal saline to dilute excrement, and the heating device is used for heating the normal saline and the excrement filtrate in the first centrifugal cavity to a specified temperature and maintaining the specified temperature;
when the stirring device in the first centrifugal cavity works for a first standard time, the stirring device stops working, the first control valve is opened, liquid in the first centrifugal cavity flows into the second centrifugal cavity after being filtered by the nanoscale filter screen, and residues and large granular substances in excrement filtrate stay in the first centrifugal cavity;
step 3, the second liquid level sensor group starts to detect the liquid level height in the second centrifugal cavity, when the second real-time liquid level height measured by the second liquid level sensor group meets a first liquid level standard value, the first control valve is closed, and at the moment, the first centrifugal cavity enters an emptying operation;
step 4, filtrate in the first centrifugal cavity enters a second centrifugal cavity, when a second real-time liquid level height measured by a second liquid level sensor group meets a first liquid level standard value, the second centrifugal cavity starts to perform centrifugal work, after a second standard time, the second control valve is opened, and primary centrifugal precipitate in the second centrifugal cavity enters a transfer cavity;
step 5, a third liquid level sensor group starts to detect the liquid level height in the transfer cavity, when the third real-time liquid level height detected by the third liquid level sensor group meets a second liquid level standard value, the second control valve is closed, and at the moment, the second centrifugal cavity enters an emptying operation to discharge supernatant liquid left in the cavity;
step 6, the first liquid level sensor group starts to detect the liquid level height in the first centrifugal cavity, when the first liquid level sensor group detects that the liquid level in the first centrifugal cavity is zero, the controller controls the stop valve to be opened, meanwhile, the controller also controls the conveying pump to work, and secondary centrifugal sediments in the transfer cavity return to the first centrifugal cavity under the action of the conveying pump;
step 7, the first liquid level sensor group continues to detect the liquid level height in the first centrifugal cavity, and when the first real-time liquid level height measured by the first liquid level sensor group meets a third liquid level standard value, the stop valve is closed;
step 8, when the third real-time liquid level height measured by the first liquid level sensor group meets a third liquid level standard value, the first centrifugal cavity starts to perform centrifugal work, after a second standard time, the first control valve is opened again, and the third centrifugal precipitate enters the second centrifugal cavity; the second liquid level sensor group detects the liquid level height in the second centrifugal cavity again, when the second real-time liquid level height detected by the second liquid level sensor group meets a fourth liquid level standard value, the first control valve is closed, the intermediate output device starts to work, the output control valve is opened, so that the normal saline in the output storage bin flows into the second centrifugal cavity, when the second real-time liquid level height detected by the second liquid level sensor group meets a fifth liquid level standard value, the intermediate output device stops working, the second centrifugal cavity performs centrifugal work again, and the heating device heats the liquid in the second centrifugal cavity to a specified temperature;
and 9, after the second centrifugal cavity enters a stirring mode to work for a third standard time, the second control valve is opened, liquid in the second centrifugal cavity flows into the transfer cavity, the third liquid level sensor group starts to detect the liquid level height in the transfer cavity, when the third real-time liquid level height detected by the third liquid level sensor group meets a sixth liquid level standard value, the second control valve is closed, and the fecal bacteria suspension meeting the requirements is stored in the transfer cavity.
The intestinal excrement detection system is high in intelligent degree, and workers can complete the preparation of the fecal bacteria only by inputting state quantity, so that the working intensity of the workers is greatly reduced, and the preparation efficiency is improved; in the whole process of preparing the fecal fungi, the raw materials are operated in the preparation system in the whole process, the whole process is closed, the embarrassing situation that workers directly contact the fecal fungi is avoided, and meanwhile, a device for cleaning and disinfecting is also arranged in the control system to meet the disinfection requirement in the preparation link.
Drawings
In order that the advantages of the invention will be readily understood, a more particular description of the invention briefly described above will be rendered by reference to specific embodiments that are illustrated in the appended drawings. Understanding that these drawings depict only typical embodiments of the invention and are not therefore to be considered to be limiting of its scope, the invention will be described and explained with additional specificity and detail through the use of the accompanying drawings.
Fig. 1 is a schematic top view of a detection system for intestinal stool according to the present invention;
FIG. 2 is a partial cross-sectional view of a bowel stool detection system according to the present invention;
FIG. 3 is a schematic front view of the intestinal stool detection system according to the present invention;
FIG. 4 is a schematic structural view of a heating apparatus according to the present invention;
FIG. 5 is a schematic structural view of a stirring device according to the present invention;
FIG. 6 is a schematic structural diagram of the test paper clamping device according to the present invention;
FIG. 7 is a schematic view of the construction of the sealer illustrated in FIG. 6;
fig. 8 is a schematic structural diagram of a control system according to the present invention.
Description of reference numerals:
1: first centrifuge tank 2: second centrifugal tank
3: a diversion pipeline 4: first control valve
5: intermediate output device 61: front return pipeline
62: rear return line 63: flow stop valve
7: transfer pot 71: transfer chamber
72: finished product output pipeline
8: second control valve 9: delivery pump
10: the controller 11: first centrifugal chamber
12: first liquid inlet/outlet port 13: test paper clamping device
21: second centrifugal chamber 22: second liquid inlet and outlet
111: the water filling bin 112: cold water pipeline
113: electric heating plate
131: the sealer 132: paper sticking roller
133: positioning clamping plate
141: rotating shaft 142: stirring sheet
143: electrical machine
1511: first heating lamp 1512: second heating lamp
1513: third add light 1514: fourth heating lamp
1511': fifth heating lamp 1512': sixth heating lamp
1513': seventh heating lamp 1514': eighth heating lamp
152: lamp control device
51: output storage bin 52: output control valve
53: output control pipe 101: first temperature sensor group
102: second temperature sensor group 103: third temperature sensor group
104: first flow rate sensor group 105: second flow rate sensor group
106: the third flow rate sensor group 107: first liquid level sensor group
108: the second liquid level sensor group 109: third liquid level sensor group
Detailed Description
In the following description, numerous specific details are set forth in order to provide a more thorough understanding of the present invention. It will be apparent, however, to one skilled in the art, that embodiments of the present invention may be practiced without one or more of these specific details. In other instances, well-known features have not been described in order to avoid obscuring embodiments of the present invention.
In the following description, a detailed structure will be presented for a thorough understanding of embodiments of the invention. It is apparent that the implementation of the embodiments of the present invention is not limited to the specific details familiar to those skilled in the art. The following detailed description of preferred embodiments of the invention, however, the invention is capable of other embodiments in addition to those detailed.
In the description of the present invention, the terms "inside", "outside", "longitudinal", "transverse", "upper", "lower", "top", "bottom", and the like indicate orientations or positional relationships based on those shown in the drawings, and are for convenience only to describe the present invention without requiring the present invention to be necessarily constructed and operated in a specific orientation, and thus, should not be construed as limiting the present invention.
Referring to fig. 1, 2 and 3, the intestinal fecal detection system according to the present invention includes a first centrifugal tank 1, a second centrifugal tank 2, a diversion pipeline 3, a first control valve 4, an intermediate output device 5, a front return pipeline 61, a rear return pipeline 62 and a transfer tank 7; the diversion pipeline 3 is connected with the first centrifugal tank 1 and the second centrifugal tank 2, and the first control valve 4 and the intermediate output device 5 are both arranged on the diversion pipeline 3; the first centrifugal chamber 11 and the second centrifugal chamber 21 are respectively arranged in the first centrifugal tank 1 and the second centrifugal tank 2, the first centrifugal chamber 11 is communicated with the second centrifugal chamber 12 through the guide pipe 3, and the horizontal height of the first centrifugal chamber 11 is higher than the horizontal height of the second centrifugal chamber 21, that is, the guide pipe 3 is arranged between the first centrifugal chamber 11 and the second centrifugal chamber 21 with a certain gradient, so that the solution in the first centrifugal chamber 11 can enter the second centrifugal chamber 21 through the guide pipe 3 only under the action of gravity. A filter screen (not shown in the figure) is arranged between the first control valve 4 and the first centrifugal chamber 11 for filtering the liquid flowing out of the first centrifugal chamber 11; the intermediate output device 5 includes an output storage bin 51, an output control valve 52 and an output control pipeline 53; the output storage chamber 51 communicates with the pilot line 3 through an output control line 53, and an output control valve 52 is provided on the output control line 53. A transfer cavity 71 is arranged in the transfer tank 7, and the front return pipeline 61 is communicated with the second centrifugal cavity 21 and the transfer cavity 71; the rear return pipeline 62 is communicated with the transfer cavity 71 and the first centrifugal cavity 11; wherein, the level of the second centrifugal cavity 21 is higher than the level of the transfer cavity 71, that is, the front return pipe 61 has a certain slope and is arranged between the second centrifugal cavity 21 and the transfer cavity 71, so that the solution in the second centrifugal cavity 21 can enter the transfer cavity 71 through the front return pipe 61 only under the action of gravity; the second control valve 8 is arranged near the interface of the front return pipe 61 and the second centrifugal cavity 21 and is used for controlling the on-off of the second centrifugal cavity 21 and the front return pipe 61; a delivery pump 9 is arranged on the rear return pipeline 62 to deliver the material in the transfer cavity 7 to the first centrifugal cavity 11; a check valve 63 is arranged at the joint of the first centrifugal cavity 11 and the rear return pipeline 62, and the check valve 63 is opened along with the opening of the conveying pump 9 and closed along with the closing of the conveying pump 9 so as to prevent the material in the first centrifugal cavity 11 from flowing back to the transit cavity 71.
With continued reference to fig. 1 and 2, the bottom of the first centrifuge tank 1 is provided with a stirring device extending to the middle of the first centrifugal chamber 11, and the top of the first centrifuge tank 1 is provided with a test paper clamping device 13; in the present invention, the stirring device, the test paper clamping device and the heating device which are arranged in the first centrifugal tank 1, the second centrifugal tank 2 and the transfer tank 7 are all the same, so the stirring device, the test paper clamping device and the heating device in the first centrifugal tank 1 are only explained in detail in the following, and similar devices in other tank bodies are not described in too much detail.
Specifically, the stirring device includes a motor 143, a rotating shaft 141, and a stirring sheet 142, where the stirring sheet 142 is disposed on the rotating shaft 141, and the motor 143 drives the rotating shaft 141 to rotate, so that the stirring sheet 142 stirs the material in the first centrifugal cavity 11.
In some embodiments of the present invention, as shown in fig. 4, the first centrifuge tank may further include a motor 143 ' for driving the first centrifugal chamber 11 to rotate, the motor 143 ' is connected to the centrifugal chamber 11 via a rotating shaft 141 ', and the motor 143 ' drives the rotating shaft 141 ' to rotate so as to achieve an agitation effect on the first centrifugal chamber 11.
As shown in fig. 5, 6 and 7, the test paper clamping device 13 includes a sealing device 131, a paper-sticking roller 132 and a positioning clamping plate 133; the sealing device 131 can rotate, when the sealing device 131 is rotated and opened, the first centrifugal cavity 11 is communicated with the outside, otherwise, the first centrifugal cavity 11 is isolated from the outside; the positioning clamping plates 133 comprise two clamping plates arranged on the sealing device 131; the pick-up roller 132 is disposed above the positioning chucking plate 133.
Specifically, the test paper clamping device 13 is used for placing PH test paper for measuring a relevant index in the first centrifugal cavity 11, when the PH test paper is placed, the lower end of the test paper goes deep into a gap between the two clamping plates, meanwhile, part of the test paper is adhered to the paper adhering roller 132, when the device enters a measuring state, the sealing device 131 is rotated and opened, the paper adhering roller 132 rotates, and the PH test paper moves downwards under the action of the paper adhering roller 132 and the positioning clamping plate 133 to be in contact with liquid in the first centrifugal cavity 11, so that detection is started; after the detection is completed, the paper-sticking roller 132 rotates in the opposite direction to take out the PH test paper in the first centrifugal chamber 11, and the sealing device 131 rotates and closes after all the PH test paper leaves the first centrifugal chamber 11.
Further, the sealing device 131 is further provided with a vent hole to ensure that the pressure in the first centrifugal tank 1, the second centrifugal tank 2 and the transfer tank 7 is always the same as the external standard atmospheric pressure.
In particular, the heating device 15 comprises a plurality of heating lamps, which are vertically arranged in the first centrifuge tank 1 and annularly distributed around the first centrifuge chamber 11; in the embodiment of the present invention, the heating device 15 includes a first heating lamp 1511, a second heating lamp 1512, a third heating lamp 1513, a fourth heating lamp 1514, a fifth heating lamp 1511 ', a sixth heating lamp 1512', a seventh heating lamp 1513 ', an eighth heating lamp 1514', and a lamp control device 152; in some embodiments of the invention, the heating device 15 operates in two modes: (1) the lamp control device 152 controls the first heating lamp 1511, the third heating lamp 1513, the fifth heating lamp 1511 ', and the seventh heating lamp 1513' to operate, and the remaining heating lamps do not operate. (2) The lamp control device 152 controls the operation of all the heating lamps. It is obvious that the different operation modes of the heating device 15 of the present invention are designed to have different heating efficiencies, and those skilled in the art can understand that the heating power of each heating lamp of the present invention can be adjusted, and the heating efficiency of the above operation mode (2) is significantly greater than that of the operation mode (1) on the premise that the heating powers of all the heating lamps are the same. The heating means 15 installed in the second centrifugation tank 2 are identical to the heating means in the first centrifugation tank 1 and the invention will not be described in more detail here.
Referring to fig. 8, it is a temperature control device according to another embodiment of the present invention, wherein a water filling bin 111 is disposed in an interlayer between the first centrifugal chamber 11 and the first centrifugal tank 1, the water filling bin 111 is a chamber body with an arc surface, and at least two water filling bins 111 are disposed on an outer chamber wall of the first centrifugal chamber 11; at least one cold water pipeline 112 is arranged in each water filling bin 111; one arc-shaped surface of the water filling bin 111 is welded with the outer side chamber wall of the first centrifugal chamber 111, and an electric heating plate 113 is arranged on the other arc-shaped surface; wherein, cold water pipeline 112 is filled with cold water to cool the hot water in the water filling bin 111, electric heating plate 113 is used for heating the water in the water filling bin 111, and cold water pipeline 112 and electric heating plate 113 act together to control the water temperature in the water filling bin 111. It will be understood by those skilled in the art that the water filling bin 111 and the cold water pipeline 112 are provided with a water inlet and a water outlet which are communicated with the outside.
As shown in the figure 8, the invention is also provided with a control system to solve the problems of complicated preparation means and low intelligent degree of the existing fecal strain preparation device. The control system comprises a control group and a controlled element. Wherein, the control group comprises a controller 10 and a control panel (not shown in the figure), the controller 10 and the control panel can be arranged on the excrement preparation system of the invention, and can also be arranged in other places which are beneficial to the control of workers, and the controller 10 is electrically connected with the control panel. In some embodiments of the present invention, a signal transceiver may be further disposed on the control panel and the controller 10, so that the control panel and the controller 10 can access a local area network or the internet. The controller 10 is a PLC controller provided on the detection device, and the control panel can remotely control the operation of the controller 10 through internet communication. The controlled elements are respectively electrically connected with the controller 10, and comprise a first temperature sensor group 101, a second temperature sensor group 102, a third temperature sensor group 103, a first flow rate sensor group 104, a second flow rate sensor group 105, a third flow rate sensor group 106, a first liquid level sensor group 107, a second liquid level sensor group 108, a third liquid level sensor group 109, a lamp control device 152, a sealer 131, a paper sticking roller 132, a first control valve 4, a delivery pump 7, a second control valve 8, a flow limiting valve 9, a motor 143 and an output object control valve 52.
Specifically, a first temperature sensor group 101 is disposed in the first centrifugal chamber 11 to detect a first real-time temperature in the first centrifugal chamber 11; the second temperature sensor group 102 is arranged in the second centrifugal cavity to detect a second real-time temperature in the second centrifugal cavity 21; the third temperature sensing group 103 is arranged in the transfer cavity to detect a third real-time temperature in the transfer cavity 71; the first flow sensor group 104 is arranged on the diversion pipeline 3 and at the front end of the intermediate output device 5 to detect the real-time flow rate V1 of the material flowing out of the first centrifugal cavity 11; a second flow sensor set 105 is disposed on the output control conduit 53 to detect a real-time flow rate V2 of the intermediate; the third flow sensor group 106 is arranged on the rear return pipeline 62 to detect the real-time flow velocity V3 of the material flowing out of the transfer chamber 71; first level sensor set 107 is disposed within first centrifugal chamber 11 to detect a real-time level height H1 of first centrifugal chamber 11; a second liquid level sensor group 108 is arranged in the second centrifugal cavity 21 to detect the real-time liquid level height H2 of the second centrifugal cavity 21, and a third liquid level sensor group 109 is arranged in the transfer cavity 71 to detect the real-time liquid level height H3 in the transfer cavity 71; the sensor group can be one sensor or a plurality of same sensors, the sensors are distributed according to a certain array, and the value obtained by the sensor group is the average value of the values measured by the sensors.
In some embodiments of the present invention, a camera (not shown) is further provided, the camera is disposed in the area enclosed by the first centrifuge tank 1, the second centrifuge tank 2 and the intermediate tank 7, the camera may be provided with three cameras, and the cameras face the test paper clamping and placing device 13 of the first centrifuge tank 1, the second centrifuge tank 2 and the intermediate tank 7 respectively; correspondingly, the test paper on the test paper clamping device 13 also faces the camera, so that the camera can feed back the data on the test paper to the computer.
With continuing reference to fig. 1-8, the present invention provides a method for preparing fecal bacteria using a fecal intestinal detection system, comprising the following steps:
step 1, adding physiological saline and excrement filtrate into a first centrifugal cavity;
step 2, a stirring device of the first centrifugal cavity enters a stirring mode, a heating device in the first centrifugal cavity starts to work, the stirring device is used for mixing excrement filtrate and normal saline to dilute excrement, and the heating device is used for heating the normal saline and the excrement filtrate in the first centrifugal cavity to a specified temperature and maintaining the specified temperature;
when the stirring device in the first centrifugal cavity works for a first standard time, the stirring device stops working, the first control valve is opened, liquid in the first centrifugal cavity flows into the second centrifugal cavity after being filtered by the nanoscale filter screen, and residues and large granular substances in excrement filtrate stay in the first centrifugal cavity;
step 3, the second liquid level sensor group starts to detect the liquid level height in the second centrifugal cavity, when the second real-time liquid level height measured by the second liquid level sensor group meets a first liquid level standard value, the first control valve is closed, and at the moment, the first centrifugal cavity enters an emptying operation;
step 4, filtrate in the first centrifugal cavity enters a second centrifugal cavity, when a second real-time liquid level height measured by a second liquid level sensor group meets a first liquid level standard value, the second centrifugal cavity starts to perform centrifugal work, after a second standard time, the second control valve is opened, and primary centrifugal precipitate in the second centrifugal cavity enters a transfer cavity;
step 5, a third liquid level sensor group starts to detect the liquid level height in the transfer cavity, when the third real-time liquid level height detected by the third liquid level sensor group meets a second liquid level standard value, the second control valve is closed, and at the moment, the second centrifugal cavity enters an emptying operation to discharge supernatant liquid left in the cavity;
step 6, the first liquid level sensor group starts to detect the liquid level height in the first centrifugal cavity, when the first liquid level sensor group detects that the liquid level in the first centrifugal cavity is zero, the controller controls the stop valve to be opened, meanwhile, the controller also controls the conveying pump to work, and secondary centrifugal sediments in the transfer cavity return to the first centrifugal cavity under the action of the conveying pump;
step 7, the first liquid level sensor group continues to detect the liquid level height in the first centrifugal cavity, and when the first real-time liquid level height measured by the first liquid level sensor group meets a third liquid level standard value, the stop valve is closed;
step 8, when the third real-time liquid level height measured by the first liquid level sensor group meets a third liquid level standard value, the first centrifugal cavity starts to perform centrifugal work, after a second standard time, the first control valve is opened again, and the third centrifugal precipitate enters the second centrifugal cavity; the second liquid level sensor group detects the liquid level height in the second centrifugal cavity again, when the second real-time liquid level height detected by the second liquid level sensor group meets a fourth liquid level standard value, the first control valve is closed, the intermediate output device starts to work, the output control valve is opened, so that the normal saline in the output storage bin flows into the second centrifugal cavity, when the second real-time liquid level height detected by the second liquid level sensor group meets a fifth liquid level standard value, the intermediate output device stops working, the second centrifugal cavity performs centrifugal work again, and the heating device heats the liquid in the second centrifugal cavity to a specified temperature;
and 9, after the second centrifugal cavity enters a stirring mode to work for a third standard time, the second control valve is opened, liquid in the second centrifugal cavity flows into the transfer cavity, the third liquid level sensor group starts to detect the liquid level height in the transfer cavity, when the third real-time liquid level height detected by the third liquid level sensor group meets a sixth liquid level standard value, the second control valve is closed, and the fecal bacteria suspension meeting the requirements is stored in the transfer cavity.
Specifically, the liquid level values stored in the controller 10 include a liquid level standard value and a time standard value; wherein, the liquid level standard value includes:
a first criterion value of H1, which is determined by the amount of saline added to first chamber 11;
a second level criterion H2, the second level criterion being determined by the first level criterion;
a third level criterion value H3, the third level criterion value being determined by the second level criterion value;
a fourth liquid level standard value H4, wherein the fourth liquid level standard value is determined by the third liquid level standard value;
a fifth level criterion value H5, which is determined by the amount of saline discharged from the intermediate output device 5;
a sixth liquid level standard value, which is determined by the fifth standard liquid level value;
the standard time value T includes:
a first standard time T1, the first standard time being determined by the addition amount of the physiological saline;
a second standard time T2, the second standard time being determined by the fifth liquid level standard value;
a third standard time T3, the third standard time being determined by a sixth liquid level standard value;
the stirring mode comprises the following steps: stirring and precipitating, wherein the stirring device stops working after working for T/2 time, and the liquid in the centrifugal cavity or the middle rotating cavity continues precipitating for T/2 time.
With continued reference to fig. 1 to 3, a first liquid inlet/outlet port 12 is provided at the outer side of the first centrifugal tank 1; a second liquid inlet and outlet 22 is arranged on the outer side of the second centrifugal tank 2; a finished product output pipeline 72 is arranged outside the transfer tank 7, and a nano-scale filter screen (not shown in the figure) is arranged in the finished product output pipeline; the evacuation operation includes: a waste discharging stage and a high-temperature disinfection stage in the cavity; when the second cavity body enters an emptying operation, the liquid inlet and outlet are opened, residues in the cavity body flow out of the centrifugal cavity or the transfer cavity along the liquid inlet and outlet, and when the liquid level sensor group detects that the liquid level in the centrifugal cavity is not changed, clear water is injected into the centrifugal cavity through the liquid inlet and outlet for cleaning; after the clear water enters the centrifugal cavity, the liquid inlet and outlet is closed, the stirring device starts to stir, the stirring device stops working after stirring for a certain time, and the liquid inlet and outlet is opened again to discharge waste liquid. The waste discharge stage in the chamber specified in the invention comprises that the centrifugal chamber continuously performs the cleaning operation for three times; after the waste in the discharge cavity stage is completed, the centrifugal cavity enters a high-temperature disinfection stage: the heating device 15 performs high-temperature sterilization on the centrifugal cavity, and the heating device 15 heats the centrifugal cavity in a high-temperature state for three minutes to achieve the sterilization effect.
In some embodiments of the invention, the liquid level criterion value and the time criterion value are calculated as follows:
the calculation method of the first liquid level standard value H1 comprises the following steps: h1 ═ QGeneral assembly- Δ 1, wherein QGeneral assemblyTo add the amount of physiological saline, Δ 1 is the flow loss of the material through the diversion conduit 3.
As will be understood by those skilled in the art, QGeneral assemblyDepending on the amount of fecal filtrate added, QGeneral assemblyCan be 3-4 times of the volume of the feces filtrate.
The method for calculating the second liquid level standard value H2 comprises the following steps: h2 ═ QGeneral assembly- Δ 1- Δ 2- α, wherein Δ 2 isThe flow loss of the material through the front return pipe 61, alpha is the volume of the supernatant and is in linear relation with the added volume of the fecal filtrate.
The calculation method of the third liquid level standard value H3 comprises the following steps: h3 ═ H2- Δ 3-1.5 α, where Δ 3 is the loss of material through the post-return line 62.
The calculation of the fourth level criterion value H4 includes: h4 ═ H3-2 α.
The calculation mode of the fifth liquid level standard value H5 comprises the following steps: h5 ═ H4- Δ 1-1.7 α.
The calculation of the sixth level criterion value H6 includes: h6 ═ H5- Δ 2+ kQGeneral assemblyWhere k is a fixed coefficient, the specific value of k is determined by the operator, kQGeneral assemblyThe volume of saline fed into the second centrifugal chamber 21 by the intermediate delivery device 5.
The first standard time T1 is calculated by: t1 ═ 3+ QGeneral assemblyAnd/n, wherein n is the number of time calculation standards, and the unit of T1 is minutes.
The second standard time T2 is calculated by: t2 ═ 2+ H5/n, and T2 units are minutes.
The third standard time T3 is calculated by: t3 ═ 2+ H6/n, and T3 units are minutes.
The control method of the intestinal fecal detection system provided by the invention also comprises a system detection method, and the detection method comprises the following steps:
step 1, calculating the flow L of the pipeline, wherein the flow calculation formula is as follows:
Figure BDA0002279549090000181
wherein S is the cross-sectional area of the pipeline, V is the real-time flow rate of the material, and T is the time of flowing through the pipeline;
substituting the cross-sectional area S1 of the guide pipeline, the real-time flow velocity V1 measured by the first flow velocity sensor group and the time T1 of the material passing through the first flow velocity sensor group into a flow calculation formula to calculate a first flow L1;
substituting the cross-sectional area S2 of the output control pipeline, the real-time flow velocity V2 measured by the second flow sensor group and the time T2 of the material passing through the second flow sensor group into a flow calculation formula to calculate a second flow L2;
substituting the cross-sectional area S3 of the front reflux pipeline, the real-time flow velocity V3 measured by the third flow velocity sensor group and the time T3 of the material passing through the third flow velocity sensor group into a flow calculation formula to calculate a third flow L3;
the working state of the intestinal stool detection system is judged by detecting the first flow L1, the second flow L2 and the third flow L3 by a worker, and the detection formula comprises the following steps: β (Q1/Q2) ═ Q3- Δ 4, where β is the centrifuge coefficient and Δ 4 is the loss difference.
If the value calculated by equation β (Q1/Q2) is not equal to the value calculated by equation Q3- Δ 4, then the system is proven to be defective and needs to be shut down for repair.
In some embodiments of the present invention, the transfer tank 7 may further include a refrigeration device, and the refrigeration device starts to refrigerate after the prepared fecal bacteria suspension enters the transfer tank 7 so as to maintain the temperature in the cavity at-80 ℃, thereby realizing preservation of the fecal bacteria suspension.
In some embodiments of the present invention, the input state quantities of the controller 10 include: volume Q of initial addition of physiological salineGeneral assemblySupernatant volume α, fixed coefficient k and loss difference Δ 4; the input state quantity of the controller 10 can be changed by the operator through the control panel.
The invention has been described by way of the above embodiments, but it is to be understood that the above embodiments are for purposes of illustration and description only and are not intended to limit the invention to the described embodiments. It will be appreciated by those skilled in the art that many variations and modifications may be made to the teachings of the invention, which fall within the scope of the invention as claimed.

Claims (10)

1. A detection system for intestinal feces is characterized in that the detection method of the system comprises the following steps:
step 1, adding physiological saline and excrement filtrate into a first centrifugal cavity;
step 2, a stirring device of the first centrifugal cavity enters a stirring mode, a heating device in the first centrifugal cavity starts to work, the stirring device is used for mixing excrement filtrate and normal saline to dilute excrement, and the heating device is used for heating the normal saline and the excrement filtrate in the first centrifugal cavity to a specified temperature and maintaining the specified temperature;
when the stirring device in the first centrifugal cavity works for a first standard time, the stirring device stops working, the first control valve is opened, liquid in the first centrifugal cavity flows into the second centrifugal cavity after being filtered by the nanoscale filter screen, and residues and large granular substances in excrement filtrate stay in the first centrifugal cavity;
step 3, a second liquid level sensor group starts to detect the liquid level height in the second centrifugal cavity, when a second real-time liquid level height detected by the second liquid level sensor group meets a first liquid level standard value, the first control valve is closed, and at the moment, the first centrifugal cavity enters an emptying operation;
step 4, filtrate in the first centrifugal cavity enters a second centrifugal cavity, when a second real-time liquid level height measured by a second liquid level sensor group meets a first liquid level standard value, the second centrifugal cavity starts to perform centrifugal work, after a second standard time, a second control valve is opened, and primary centrifugal precipitate in the second centrifugal cavity enters a transfer cavity;
step 5, a third liquid level sensor group starts to detect the liquid level height in the transfer cavity, when the third real-time liquid level height detected by the third liquid level sensor group meets a second liquid level standard value, the second control valve is closed, and at the moment, the second centrifugal cavity enters an emptying operation to discharge supernatant liquid left in the cavity;
step 6, a first liquid level sensor group starts to detect the liquid level height in the first centrifugal cavity, when the first liquid level sensor group detects that the liquid level in the first centrifugal cavity is zero, a controller controls a stop valve to be opened, meanwhile, the controller also controls a conveying pump to work, and secondary centrifugal sediments in the transfer cavity return to the first centrifugal cavity under the action of the conveying pump;
step 7, the first liquid level sensor group continues to detect the liquid level height in the first centrifugal cavity, and when the first real-time liquid level height detected by the first liquid level sensor group meets a third liquid level standard value, the stop valve is closed;
step 8, when the third real-time liquid level height measured by the first liquid level sensor group meets a third liquid level standard value, the first centrifugal cavity starts to perform centrifugal work, after a second standard time, the first control valve is opened again, and the third centrifugal precipitate enters the second centrifugal cavity; the second liquid level sensor group detects the liquid level height in the second centrifugal cavity again, when the second real-time liquid level height detected by the second liquid level sensor group meets a fourth liquid level standard value, the first control valve is closed, the intermediate output device starts to work, the output control valve is opened, so that the normal saline in the output storage bin flows into the second centrifugal cavity, when the second real-time liquid level height detected by the second liquid level sensor group meets a fifth liquid level standard value, the intermediate output device stops working, the second centrifugal cavity performs centrifugal work again, and the heating device heats the liquid in the second centrifugal cavity to a specified temperature;
and 9, after the second centrifugal cavity enters a stirring mode to work for a third standard time, the second control valve is opened, liquid in the second centrifugal cavity flows into the transfer cavity, the third liquid level sensor group starts to detect the liquid level height in the transfer cavity, when the third real-time liquid level height detected by the third liquid level sensor group meets a sixth liquid level standard value, the second control valve is closed, and the fecal bacteria suspension meeting the requirements is stored in the transfer cavity.
2. The system for detecting intestinal fecal material according to claim 1 further comprising a fecal bacteria preparation system comprising: the device comprises a first centrifugal tank, a second centrifugal tank, a diversion pipeline, a first control valve, an intermediate output device, a front return pipeline, a rear return pipeline and a transfer tank;
the first centrifugal cavity and the second centrifugal cavity are communicated through the diversion pipeline, and the horizontal height of the first centrifugal cavity is higher than that of the second centrifugal cavity; the nanoscale filter screen is arranged between the first control valve and the first centrifugal cavity and is used for filtering liquid flowing out of the first centrifugal cavity; the intermediate output device comprises the output storage bin, the output control valve and the output control pipeline; the output material storage bin is communicated with a diversion pipeline through the output control pipeline, the output control valve is arranged on the output control pipeline, a transfer cavity is arranged in the transfer tank, and a front return pipeline is communicated with the second centrifugal cavity and the transfer cavity; the rear return pipeline is communicated with the transfer cavity and the first centrifugal cavity; the second control valve is arranged near a joint of the front return pipeline and the second centrifugal cavity and used for controlling the connection and disconnection of the second centrifugal cavity and the front return pipeline; the rear return pipeline is provided with the conveying pump so as to convey the materials in transit to the first centrifugal cavity; the connecting part of the first centrifugal cavity and the rear backflow pipeline is provided with the check valve, the check valve is opened along with the opening of the conveying pump, and the check valve is closed along with the closing of the conveying pump.
3. The system for detecting intestinal stool as defined in claim 2, wherein the standard liquid level value is calculated by:
the calculation method of the first liquid level standard value H1 comprises the following steps: h1 ═ qtol- Δ 1, where qtol is the amount of saline added and Δ 1 is the volume loss of material flowing through the flow conduit;
the method for calculating the second liquid level standard value H2 comprises the following steps: h2 ═ Q total- Δ 1- Δ 2- α, where Δ 2 is the volume loss of material flowing through the front reflux line, α is the volume of supernatant, and α is linear with the amount of fecal filtrate added;
the calculation method of the third liquid level standard value H3 comprises the following steps: h3 ═ H2- Δ 3-1.5 α, where Δ 3 is the loss of material through the post return line;
the calculation of the fourth level criterion value H4 includes: h4 ═ H3-2 α;
the calculation mode of the fifth liquid level standard value H5 comprises the following steps: h5 ═ H4- Δ 1-1.7 α;
the calculation of the sixth level criterion value H6 includes: h6 ═ H5- Δ 2+ kQ total;
wherein k is a fixed coefficient, the specific value of k is determined by a worker, and kQ is always the volume of the physiological saline input into the second centrifugal cavity by the intermediate output device.
4. The system for detecting bowel movement according to claim 2, wherein the time criterion is calculated by:
the first standard time T1 is calculated by: t1 ═ 3+ Q total/n, where n is a positive integer and T1 is in minutes;
the second standard time T2 is calculated by: t2 ═ 2+ H5/n, units of T2 are minutes;
the third standard time T3 is calculated by: t3 ═ 2+ H6/n, and T3 units are minutes.
5. The intestinal fecal detection system according to claim 2, wherein the bottom of the centrifuge tank is provided with a stirring device extending to the middle of the centrifuge chamber, and the top of the centrifuge tank is provided with a test paper clamping device; and a heating device is arranged in a space enclosed between the outer surface of the centrifugal cavity and the inner wall of the centrifugal tank.
6. The intestinal fecal detection system according to claim 5, wherein the first centrifuge tank is provided with a first liquid inlet and outlet on the outer side; a second liquid inlet and outlet is formed in the outer side of the second centrifugal tank; the transfer tank is provided with a third liquid inlet and outlet;
the emptying operation comprises a waste discharging stage in the cavity and a high-temperature sterilizing stage;
when the second cavity body enters an emptying operation, the liquid inlet and outlet is opened, residues in the cavity body flow out of the centrifugal cavity or the transfer cavity along the liquid inlet and outlet, and when the liquid level sensor group detects that the liquid level in the centrifugal cavity is stable, clear water is injected into the centrifugal cavity through the liquid inlet and outlet for cleaning; after clear water enters the centrifugal cavity, the liquid inlet and outlet are closed, the stirring device starts stirring, the stirring device stops working after stirring for a certain time, and the liquid inlet and outlet are opened again to discharge waste liquid; and after the stage of discharging wastes in the cavity is finished, the centrifugal cavity enters a high-temperature sterilization stage, wherein the centrifugal cavity is subjected to high-temperature sterilization by the heating device, and the centrifugal cavity is heated for three minutes in a high-temperature state by the heating device so as to achieve a sterilization effect.
7. The intestinal fecal detection system according to claim 5, further comprising a camera, wherein the camera is arranged in the area enclosed by the first centrifuge tank, the second centrifuge tank and the intermediate tank, the camera is provided with three cameras, the cameras are respectively facing the test paper clamping devices of the first centrifuge tank, the second centrifuge tank and the intermediate tank, so that the cameras feed back the data on the test paper to the computer.
8. The system for detecting bowel movement according to claim 2, further comprising a control system, said control system including a control group and a controlled element;
wherein, the control group includes controller and control panel, the controller electricity is connected control panel, the controller is controlled the component through the control and in order to realize control function, controlled the component includes first temperature sensor group, second temperature sensor group, third temperature sensor group, first flow sensor group, second flow sensor group, third flow sensor group first liquid level sensor group second liquid level sensor group third liquid level sensor group, lamps and lanterns controlling means, sealer, glue paper roll first control valve the delivery pump second control valve, restriction valve, motor and output thing control valve.
9. The system for detecting bowel movement according to claim 8, wherein said first set of temperature sensors is disposed within said first centrifugal chamber for detecting a first real-time temperature within said first centrifugal chamber; the second temperature sensor group is arranged in the second centrifugal cavity to detect a second real-time temperature in the second centrifugal cavity; the third temperature sensing group is arranged in the transfer cavity to detect a third real-time temperature in the transfer cavity; the first flow sensor group is arranged on a diversion pipeline and at the front end of the intermediate output device so as to detect the real-time flow velocity V1 of the material flowing out of the first centrifugal cavity; the second flow sensor group is arranged on the output control pipeline to detect the real-time flow rate V2 of the intermediate; the third flow rate sensor group is arranged on the rear return pipeline to detect the real-time flow rate V3 of the material flowing out of the transfer cavity; the first liquid level sensor group is arranged in the first centrifugal cavity to detect a first real-time liquid level height of the first centrifugal cavity; the second liquid level sensor group is arranged in the second centrifugal cavity to detect the second real-time liquid level height of the second centrifugal cavity, and the third liquid level sensor group is arranged in the transfer cavity to detect the third real-time liquid level height in the transfer cavity.
10. The system for detecting intestinal stool according to claim 3, 4 or 8, wherein the input state quantity of the controller includes: calculating a standard number n by the total amount Q total of the physiological saline added for the first time, the volume alpha of the supernatant, a fixed coefficient k and a loss difference delta 4 time; and the input state quantity of the controller can be changed by a worker through the control panel.
CN201911135718.0A 2019-11-19 2019-11-19 Intestinal excrement detection system Active CN110836808B (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201911135718.0A CN110836808B (en) 2019-11-19 2019-11-19 Intestinal excrement detection system

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201911135718.0A CN110836808B (en) 2019-11-19 2019-11-19 Intestinal excrement detection system

Publications (2)

Publication Number Publication Date
CN110836808A CN110836808A (en) 2020-02-25
CN110836808B true CN110836808B (en) 2022-05-10

Family

ID=69576749

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201911135718.0A Active CN110836808B (en) 2019-11-19 2019-11-19 Intestinal excrement detection system

Country Status (1)

Country Link
CN (1) CN110836808B (en)

Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105624027A (en) * 2014-10-31 2016-06-01 南京法迈特科技发展有限公司 Intelligent separation system and method for microbes in cavity contents
CN106243217A (en) * 2016-08-30 2016-12-21 福建贝迪药业有限公司 A kind of production system being suitable for yolk antibody and preparation technology
CN107663504A (en) * 2017-10-09 2018-02-06 宜昌喜旺食品有限公司 A kind of equipment and detection method of Intelligent Measurement milk bacterial number
WO2018039443A1 (en) * 2016-08-24 2018-03-01 Novaflux, Inc. Apparatus and methods for hygiene testing a medical device
CN109010380A (en) * 2018-08-21 2018-12-18 卓源健康科技有限公司 A kind of technique that caprophyl transplants the intestinal flora preparation in clinical treatment
CN109294952A (en) * 2018-10-23 2019-02-01 上海市第十人民医院 A kind of preparation method of intestines bacteria composition
EP3443967A1 (en) * 2017-08-17 2019-02-20 Labor L + S Aktiengesellschaft Method for depositing the bacterial flora of the gut of a living being, in particular a human being
CA3078926A1 (en) * 2017-10-09 2019-04-18 The Insides Company Limited Nutrient recycling device

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2015172255A1 (en) * 2014-05-16 2015-11-19 Qvella Corporation Apparatus, system and method for performing automated centrifugal separation
ES2924373T3 (en) * 2017-03-01 2022-10-06 Fluidion Field Deployable Multiplexed Monitoring and Sampling Device and Bacterial Contamination Measurement Procedure

Patent Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105624027A (en) * 2014-10-31 2016-06-01 南京法迈特科技发展有限公司 Intelligent separation system and method for microbes in cavity contents
WO2018039443A1 (en) * 2016-08-24 2018-03-01 Novaflux, Inc. Apparatus and methods for hygiene testing a medical device
CN106243217A (en) * 2016-08-30 2016-12-21 福建贝迪药业有限公司 A kind of production system being suitable for yolk antibody and preparation technology
EP3443967A1 (en) * 2017-08-17 2019-02-20 Labor L + S Aktiengesellschaft Method for depositing the bacterial flora of the gut of a living being, in particular a human being
CN107663504A (en) * 2017-10-09 2018-02-06 宜昌喜旺食品有限公司 A kind of equipment and detection method of Intelligent Measurement milk bacterial number
CA3078926A1 (en) * 2017-10-09 2019-04-18 The Insides Company Limited Nutrient recycling device
CN109010380A (en) * 2018-08-21 2018-12-18 卓源健康科技有限公司 A kind of technique that caprophyl transplants the intestinal flora preparation in clinical treatment
CN109294952A (en) * 2018-10-23 2019-02-01 上海市第十人民医院 A kind of preparation method of intestines bacteria composition

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
双歧杆菌三联活菌胶囊对胆囊切除术后腹泻患者肠道菌群及 sIgA 水平的影响;王梦华;《中国微生态学杂志》;20141031;第26卷(第10期);第1166-1168页 *
粪菌移植在肠道疾病临床应用的研究进展;窦菲菲等;《山东医药》;20181231;第58卷(第18期);第99-103页 *
肠道菌群移植与溃疡性结肠炎;金博;《世界华人消化杂志》;20170108;第25卷(第1期);第23-30页 *

Also Published As

Publication number Publication date
CN110836808A (en) 2020-02-25

Similar Documents

Publication Publication Date Title
KR102383028B1 (en) Upstream and downstream processing in disposable containers
US9717842B2 (en) Disposable fluid circuits and methods for cell washing
CN102061260B (en) Medical automatic cell production device and production method thereof
US11028360B2 (en) Separator for microorganisms in cavitary contents
CN206266397U (en) A kind of environment friendly medical sewage treatment equipment
CN110141444A (en) Waste liquid collection device for internal medicine nursing
CN216916398U (en) Automatic filling equipment of high flux stem cell preparation
CN110836808B (en) Intestinal excrement detection system
CN109401962A (en) A method of preparing cell
CN110615568A (en) Case detects waste liquid processing apparatus
CN201883098U (en) Automatic production device for medical cells
CN105505760B (en) The full-automatic non-closed cell washing system of centrifugation
CN109321458A (en) A method of preparing cell
CN111573947A (en) Energy-saving environment-friendly sewage treatment device and treatment method thereof
CN105624027B (en) Microorganism intelligence piece-rate system and its method in cavity content
CN113832028A (en) Full-automatic cell culture system and culture method
CN205844052U (en) Night soil-treatment collection device
CN106237706A (en) High-efficient automatic purifying plant
CN210812960U (en) In-vivo closed-loop sterilization equipment
CN206418128U (en) A kind of circulating fermentation system
CN106929398A (en) Full-automatic caprophyl separator
CN1524513B (en) Production method and equioment for big transfusion in bag
CN218420519U (en) Drainage filter chamber
CN104489819A (en) Potato frozen juice squeezing system and potato frozen juice squeezing process
CN220724118U (en) Liquid preparation device for strain culture

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination
GR01 Patent grant
GR01 Patent grant