CN109010380A - A kind of technique that caprophyl transplants the intestinal flora preparation in clinical treatment - Google Patents
A kind of technique that caprophyl transplants the intestinal flora preparation in clinical treatment Download PDFInfo
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/66—Microorganisms or materials therefrom
- A61K35/74—Bacteria
- A61K35/741—Probiotics
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
- A61K47/06—Organic compounds, e.g. natural or synthetic hydrocarbons, polyolefins, mineral oil, petrolatum or ozokerite
- A61K47/08—Organic compounds, e.g. natural or synthetic hydrocarbons, polyolefins, mineral oil, petrolatum or ozokerite containing oxygen, e.g. ethers, acetals, ketones, quinones, aldehydes, peroxides
- A61K47/10—Alcohols; Phenols; Salts thereof, e.g. glycerol; Polyethylene glycols [PEG]; Poloxamers; PEG/POE alkyl ethers
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P1/00—Drugs for disorders of the alimentary tract or the digestive system
Abstract
The invention discloses the techniques of the intestinal flora preparation in a kind of caprophyl transplanting clinical treatment; including feces collection, the preparation of intestinal flora preparation and the big step of intestinal flora preparation filling three; wherein feces collection includes collecting dung and anaerobism transport; the preparation of intestinal flora preparation includes the filtering step by step under the conditions of " aerobic+anaerobism "; mixed configuration after centrifugation, intestinal flora preparation are filling using glycerol protection liquid and the storage of Cryopreserved technology；The present invention uses the novel separation method of a kind of " aerobic+anaerobism " when separating intestinal flora liquid; according to intestinal flora living environment feature; select the separation method of " aerobic+anaerobism " that can utmostly protect in enteron aisle the bacterial diversity of " aerobic bacteria " and " anaerobic bacteria "; simultaneously; the glycerol protective agent of addition maintains bacterial activity within a certain period of time, improves the effect of intestinal flora preparation.
Description
Technical field
The present invention relates to medical preparation technology fields, and in particular to the enterobacteriaceae in a kind of caprophyl transplanting clinical treatment
The technique of group's preparation.
Background technique
Modern scientific research shows that intestinal flora plays a crucial role the health of human body.The enterobacteriaceae of human body
Group keeps dynamic balance state with intestinal environment, and in some cases, this balance is broken, intestinal bacilli illness, causes
Disease aggravates the state of an illness, causes complication that multiple organ dysfunction syndrome and multiple organ failure even occurs.
Intestinal flora transplanting is also known as caprophyl transplanting (Fecal microbiota transplantation, abbreviation FMT)
Functional flora in healthy human faecal mass is separated, is transplanted in Intestinal Mucosal Injury in Patients Undergoing by forms such as Gastrointestinal Endoscopes, colonoscopy, bowel lavage,
The intestinal flora of reconstruction patients realizes the technology of enteron aisle and parenterally disease treatment.
Currently, intestinal flora implantation technique is ratified by U.S. Food and Drug Administration for treating recurrent hardly possible
Distinguish the treatment of clostridial infection (Clostridium difficile infection, CDI).More basic science
Show intestinal flora implantation technique to treatment inflammatory bowel disease, colorectal cancer, irritable bowel syndrome, Crow grace with clinical research
Disease etc., the advantage for also having its original with chronic metabolic diseases such as obesity, diabetes, so the technology and reason of intestinal flora transplanting
Thought is approved by more and more people.Therefore, more and more scientific researches are wished to treat related disease with FMT technology.
As intestinal flora implantation technique is gradually spread out in clinical trial, people come to realise standardization intestinal flora
Influence of the preparation to the therapeutic effect of disease is huge.The effect of different preparation methods obtained intestinal flora preparation thousand poor ten thousand
Not.Use the time of short duration due to intestinal flora implantation technique, majority research is also in the primary stage respectively groped.Currently,
The mode for preparing intestinal flora preparation of mainstream has been broadly divided into oxygen separating on the market and anaerobism separates two kinds, aerobic lock out operation
Conveniently, equipment is simple, is measured and uses by many National People's Congress, but the dominant microflora overwhelming majority in intestinal flora is anaerobic bacteria, wherein
Part is strict anaerobes, they are easy to lose activity in aerobic environment, dead, decomposition, so there is oxygen separating completely
Method make intestinal flora loss serious.The separation method of complete anaerobism, it is complicated for operation, equipment requirement is high, and can lose
Part aerobic population.Therefore, invent a kind of flora easy to operate and that " aerobic bacteria " and " anaerobic bacteria " can be protected in intestinal flora
Multifarious intestinal flora standard preparation preparation process, which is one, technical problem to be solved.
Summary of the invention
(1) the technical issues of solving
In view of the deficiencies of the prior art, the purpose of the present invention is to provide the enterobacteriaceaes in a kind of caprophyl transplanting clinical treatment
The technique of group's preparation.
(2) technical solution
The present invention is achieved by the following technical programs:
A kind of caprophyl transplants the technique of the intestinal flora preparation in clinical treatment, including feces collection, intestinal flora preparation
Preparation and the big step of intestinal flora preparation filling three:
1) feces collection:
A, healthy donors are chosen, carry out ultraviolet lamp disinfection in sampler chamber advancing into for sampler body, and prepare sterilizing acquisition
Box, sealing homogenizing bag and anaerobic gas generation packet；
B, excrement is discharged in donor in confined space, and collects excrement with sterilizing collecting cassette, is immediately placed in after the completion of collecting
It is sealed in homogenizing bag, cold chain transportation is between laboratory operation；
2) prepared by intestinal flora preparation:
A, fresh excreta is divided into two parts in anaerobism work station, two parts of volume ratios are 2:1, and the portion that volume is less
It is transferred to synchronization process in Biohazard Safety Equipment；
B, it is added sterile saline in excrement, and is stirred with glass bar to pulverizing solid manure, then use magnetic stirring apparatus
It is stirred liquid for a period of time, forms stable suspension；
C, suspension is first slow transitted through to the filter screen of 40 mesh, removes bulky grain residue, retains filtered fluid A, then will filtering
Liquid A slow transits through the filter screen of 80 mesh, further removes particulate matter and swill, retains filtered fluid B；
D, filtered fluid B is dispensed into centrifuge tube, then using centrifuge centrifugation a period of time, removes supernatant, collect
Precipitating；
E, precipitating is resuspended with sterile saline again, stirs into suspension, and be added and go out in the suspension being stirred
1min is sufficiently stirred after bacterium glycerol；
F, suspension is prepared according to the step of a-e under aerobic conditions, and the suspension prepared under aerobic conditions is turned
It transports in anaerobism work station and is mixed with the suspension prepared under anaerobic condition, be prepared into intestinal flora liquid；
3) intestinal flora preparation is filling:
After cillin bottle sterilization, the finally obtained intestinal flora liquid of step 2) is poured into cillin bottle under anaerobic
In filling, blend compounds plug sealing, the intestinal flora preparation in a kind of caprophyl transplanting clinical treatment is obtained after labelled.
Further, the temperature of cold chain transportation is 4 DEG C in the step 1).
Further, sterile saline and the volume ratio of excrement are 4:1, the step in the b step of the step 2)
2) volume ratio of sterile saline and precipitated liquid is 2:1 in step e.
Further, the mixing speed of magnetic stirring apparatus is 800-1500rpm in the b step of the step 2), when stirring
Between be 5-10min.
Further, centrifuging temperature is 4 DEG C, centrifugal speed 20000-24000r/min in the Step d of the step 2),
Centrifugation time 5min.
Further, sterile glycerol and suspension volume ratio are 1:4-6 in the step e of the step 2).
(3) beneficial effect
The present invention is combined during preparation standardizes intestinal flora preparation using there is oxygen separating to separate with anaerobism
Mode separating feces flora, the suspension mixing filling for finally preparing both separate modes, protects enteron aisle to the full extent
The diversity of " aerobic bacteria " and " anaerobic bacteria " in flora；Meanwhile by the sterile glycerol of addition, extend the storage of intestinal flora preparation
It deposits the time, maintains bacterial activity within a certain period of time, improve the effect of intestinal flora preparation.
Specific embodiment
In order to make the object, technical scheme and advantages of the embodiment of the invention clearer, below in conjunction with the embodiment of the present invention,
Technical scheme in the embodiment of the invention is clearly and completely described, it is clear that described embodiment is the present invention one
Divide embodiment, instead of all the embodiments.Based on the embodiments of the present invention, those of ordinary skill in the art are not making
Every other embodiment obtained, shall fall within the protection scope of the present invention under the premise of creative work.
Embodiment 1:
Intestinal flora preparation in a kind of caprophyl transplanting clinical treatment, preparation step are as follows:
1) feces collection:
When receiving donor defecation notice, the ultraviolet lamp sterilization 30min for preparing collection box and being placed on feces collection room, and spray
Alcohol is spread, disinfection is spare, after donor replaces clean garment, after sterilizing 30min in the UV lamp into collection room, above collection box
After the completion of defecation, collection box external application homogeneous seal bag is sealed for defecation, and under the conditions of 4 DEG C, cold chain transportation to laboratory is waited
Processing；
2) prepared by intestinal flora preparation:
After excrement is weighed, record fecal character, weight, dat recorder get off, and assess stool quality, collect high-quality excrement
Just and to be transferred to anaerobism work station medium to be processed, takes the high-quality excrement of 90g, 60g and 30g two are classified as in anaerobism work station
Part, 30g excrement is transported to synchronization process in Biohazard Safety Equipment；
The sterile saline of 4 times of volumes is added into 60g excrement, is stirred with glass bar to pulverizing solid manure, then use
Magnetic stirring apparatus is stirred liquid 10min under 800rpm mixing speed, forms stable suspension, then that suspension is slow
By the filter screen of 40 mesh, bulky grain residue is removed, retains filtered fluid, then filtered fluid is slow transitted through to the filter screen of 80 mesh, into
One step removes particulate matter and swill, retains filtered fluid；
Filtered fluid after secondary filter is dispensed into 50ml centrifuge tube, is then put into supercentrifuge at a temperature of 4 DEG C
It is centrifuged 5min with the revolving speed of 20000r/min, removes supernatant, collects precipitating, then will be sunk with the sterile saline of 2 times of volumes
It forms sediment and is resuspended, stir into suspension, the sterile glycerol of 1/4 volume is added into the suspension being stirred and 1min is sufficiently stirred；
The 30g excrement of synchronization process is prepared into suspension according to preparation the step of a-e under aerobic conditions, and will be aerobic
Under the conditions of the suspension for preparing be transported in anaerobism work station and mixed with the suspension prepared under anaerobic condition, be prepared into enterobacteriaceae
Group's liquid；
3) intestinal flora preparation is filling:
After cillin bottle sterilization, finally obtained intestinal flora liquid is poured into cillin bottle with every under anaerobic
Bottle 40ml carries out filling, blend compounds plug sealing, and the intestinal flora in a kind of caprophyl transplanting clinical treatment is obtained after labelled
Preparation.
Embodiment 2:
Intestinal flora preparation in a kind of caprophyl transplanting clinical treatment, preparation step are as follows:
1) feces collection:
When receiving donor defecation notice, the ultraviolet lamp sterilization 30min for preparing collection box and being placed on feces collection room, and spray
Alcohol is spread, disinfection is spare, after donor replaces clean garment, after sterilizing 30min in the UV lamp into collection room, above collection box
After the completion of defecation, collection box external application homogeneous seal bag is sealed for defecation, and under the conditions of 4 DEG C, cold chain transportation to laboratory is waited
Processing；
2) prepared by intestinal flora preparation:
After excrement is weighed, record fecal character, weight, dat recorder get off, and assess stool quality, collect high-quality excrement
Just and to be transferred to anaerobism work station medium to be processed, takes the high-quality excrement of 90g, 60g and 30g two are classified as in anaerobism work station
Part, 30g excrement is transported to synchronization process in Biohazard Safety Equipment；
The sterile saline of 4 times of volumes is added into 60g excrement, is stirred with glass bar to pulverizing solid manure, then use
Magnetic stirring apparatus is stirred liquid 10min under 1500rpm mixing speed, forms stable suspension, then delays suspension
The slow filter screen for passing through 40 mesh, removes bulky grain residue, retains filtered fluid, then filtered fluid is slow transitted through to the filter screen of 80 mesh,
Particulate matter and swill are further removed, filtered fluid is retained；
Filtered fluid after secondary filter is dispensed into 50ml centrifuge tube, is then put into supercentrifuge at a temperature of 4 DEG C
It is centrifuged 5min with the revolving speed of 24000r/min, removes supernatant, collects precipitating, then will be sunk with the sterile saline of 2 times of volumes
It forms sediment and is resuspended, stir into suspension, the sterile glycerol of 1/4 volume is added into the suspension being stirred and 1min is sufficiently stirred；
The 30g excrement of synchronization process is prepared into suspension according to preparation the step of a-e under aerobic conditions, and will be aerobic
Under the conditions of the suspension for preparing be transported in anaerobism work station and mixed with the suspension prepared under anaerobic condition, be prepared into enterobacteriaceae
Group's liquid；
3) intestinal flora preparation is filling:
After cillin bottle sterilization, finally obtained intestinal flora liquid is poured into cillin bottle with every under anaerobic
Bottle 40ml carries out filling, blend compounds plug sealing, and the intestinal flora in a kind of caprophyl transplanting clinical treatment is obtained after labelled
Preparation.
Embodiment 3:
Intestinal flora preparation in a kind of caprophyl transplanting clinical treatment, preparation step are as follows:
1) feces collection:
When receiving donor defecation notice, the ultraviolet lamp sterilization 30min for preparing collection box and being placed on feces collection room, and spray
Alcohol is spread, disinfection is spare, after donor replaces clean garment, after sterilizing 30min in the UV lamp into collection room, above collection box
After the completion of defecation, collection box external application homogeneous seal bag is sealed for defecation, and under the conditions of 4 DEG C, cold chain transportation to laboratory is waited
Processing；
2) prepared by intestinal flora preparation:
After excrement is weighed, record fecal character, weight, dat recorder get off, and assess stool quality, collect high-quality excrement
Just and to be transferred to anaerobism work station medium to be processed, takes the high-quality excrement of 90g, 60g and 30g two are classified as in anaerobism work station
Part, 30g excrement is transported to synchronization process in Biohazard Safety Equipment；
The sterile saline of 4 times of volumes is added into 60g excrement, is stirred with glass bar to pulverizing solid manure, then use
Magnetic stirring apparatus is stirred liquid 5min under 1500rpm mixing speed, forms stable suspension, then that suspension is slow
By the filter screen of 40 mesh, bulky grain residue is removed, retains filtered fluid, then filtered fluid is slow transitted through to the filter screen of 80 mesh, into
One step removes particulate matter and swill, retains filtered fluid；
Filtered fluid after secondary filter is dispensed into 50ml centrifuge tube, is then put into supercentrifuge at a temperature of 4 DEG C
It is centrifuged 5min with the revolving speed of 20000r/min, removes supernatant, collects precipitating, then will be sunk with the sterile saline of 2 times of volumes
It forms sediment and is resuspended, stir into suspension, the sterile glycerol of 1/6 volume is added into the suspension being stirred and 1min is sufficiently stirred；
The 30g excrement of synchronization process is prepared into suspension according to preparation the step of a-e under aerobic conditions, and will be aerobic
Under the conditions of the suspension for preparing be transported in anaerobism work station and mixed with the suspension prepared under anaerobic condition, be prepared into enterobacteriaceae
Group's liquid；
3) intestinal flora preparation is filling:
After cillin bottle sterilization, finally obtained intestinal flora liquid is poured into cillin bottle with every under anaerobic
Bottle 40ml carries out filling, blend compounds plug sealing, and the intestinal flora in a kind of caprophyl transplanting clinical treatment is obtained after labelled
Preparation.
Contrast groups 1:
Intestinal flora preparation in a kind of caprophyl transplanting clinical treatment, preparation step are as follows:
1) feces collection:
When receiving donor defecation notice, the ultraviolet lamp sterilization 30min for preparing collection box and being placed on feces collection room, and spray
Alcohol is spread, disinfection is spare, after donor replaces clean garment, after sterilizing 30min in the UV lamp into collection room, above collection box
After the completion of defecation, collection box external application homogeneous seal bag is sealed for defecation, and under the conditions of 4 DEG C, cold chain transportation to laboratory is waited
Processing；
2) prepared by intestinal flora preparation:
After excrement is weighed, record fecal character, weight, dat recorder get off, and assess stool quality, collect high-quality excrement
Just and to be transferred to anaerobism work station medium to be processed, takes the high-quality excrement of 90g；
The sterile saline of 4 times of volumes is added into 90g excrement, is stirred with glass bar to pulverizing solid manure, then use
Magnetic stirring apparatus is stirred liquid 10min under 800rpm mixing speed, forms stable suspension, then that suspension is slow
By the filter screen of 40 mesh, bulky grain residue is removed, retains filtered fluid, then filtered fluid is slow transitted through to the filter screen of 80 mesh, into
One step removes particulate matter and swill, retains filtered fluid；
Filtered fluid after secondary filter is dispensed into 50ml centrifuge tube, is then put into supercentrifuge at a temperature of 4 DEG C
It is centrifuged 5min with the revolving speed of 20000r/min, removes supernatant, collects precipitating, then will be sunk with the sterile saline of 2 times of volumes
It forms sediment and is resuspended, stir into suspension, the sterile glycerol of 1/4 volume is added into the suspension being stirred and 1min is sufficiently stirred, make
For at intestinal flora liquid；
3) intestinal flora preparation is filling:
After cillin bottle sterilization, finally obtained intestinal flora liquid is poured into cillin bottle with every under anaerobic
Bottle 40ml carries out filling, blend compounds plug sealing, and the intestinal flora in a kind of caprophyl transplanting clinical treatment is obtained after labelled
Preparation.
Contrast groups 2:
Intestinal flora preparation in a kind of caprophyl transplanting clinical treatment, preparation step are as follows:
1) feces collection:
When receiving donor defecation notice, the ultraviolet lamp sterilization 30min for preparing collection box and being placed on feces collection room, and spray
Alcohol is spread, disinfection is spare, after donor replaces clean garment, after sterilizing 30min in the UV lamp into collection room, above collection box
After the completion of defecation, collection box external application homogeneous seal bag is sealed for defecation, and under the conditions of 4 DEG C, cold chain transportation to laboratory is waited
Processing；
2) prepared by intestinal flora preparation:
After excrement is weighed, record fecal character, weight, dat recorder get off, and assess stool quality, collect high-quality excrement
Just and to be transferred to aerobic work station medium to be processed, takes the high-quality excrement of 90g；
The sterile saline of 4 times of volumes is added into 90g excrement, is stirred with glass bar to pulverizing solid manure, then use
Magnetic stirring apparatus is stirred liquid 10min under 800rpm mixing speed, forms stable suspension, then that suspension is slow
By the filter screen of 40 mesh, bulky grain residue is removed, retains filtered fluid, then filtered fluid is slow transitted through to the filter screen of 80 mesh, into
One step removes particulate matter and swill, retains filtered fluid；
Filtered fluid after secondary filter is dispensed into 50ml centrifuge tube, is then put into supercentrifuge at a temperature of 4 DEG C
It is centrifuged 5min with the revolving speed of 20000r/min, removes supernatant, collects precipitating, then will be sunk with the sterile saline of 2 times of volumes
It forms sediment and is resuspended, stir into suspension, the sterile glycerol of 1/4 volume is added into the suspension being stirred and 1min is sufficiently stirred, make
For at intestinal flora liquid；
3) intestinal flora preparation is filling:
After cillin bottle sterilization, finally obtained intestinal flora liquid is poured into cillin bottle with every under aerobic conditions
Bottle 40ml carries out filling, blend compounds plug sealing, and the intestinal flora in a kind of caprophyl transplanting clinical treatment is obtained after labelled
Preparation.
The intestinal flora preparation of embodiment 1, embodiment 2, embodiment 3, contrast groups 1 and contrast groups 2 is put into culture dish,
And the identical culture solution of ingredient is added, it cultivates 36 hours in the culture apparatus under 37 DEG C of the same terms, then takes culture dish
Macroscopic flora number on culture dish surface is observed and recorded out, records data such as the following table 1:
The following table 1
| Embodiment | Embodiment 1 | Embodiment 2 | Embodiment 3 | Contrast groups 1 | Contrast groups 2 |
| Flora number | 16 | 18 | 15 | 12 | 2 |
From the above embodiments are merely illustrative of the technical solutions of the present invention, rather than its limitations；Although referring to aforementioned implementation
Invention is explained in detail for example, those skilled in the art should understand that: it still can be to aforementioned each reality
Technical solution documented by example is applied to modify or equivalent replacement of some of the technical features；And these modification or
Person's replacement, the spirit and scope for technical solution of various embodiments of the present invention that it does not separate the essence of the corresponding technical solution.
Claims (6)
1. a kind of technique of the intestinal flora preparation in caprophyl transplanting clinical treatment, which is characterized in that including feces collection, enteron aisle
The preparation of flora preparation and the big step of intestinal flora preparation filling three:
1) feces collection:
A, healthy donors are chosen, carry out ultraviolet lamp disinfection in sampler chamber advancing into for sampler body, and prepare the collecting cassette, close of sterilizing
Seal homogenizing bag and anaerobic gas generation packet；
B, excrement is discharged in donor in confined space, and collects excrement with sterilizing collecting cassette, and homogeneous is immediately placed in after the completion of collecting
It is sealed in bag, cold chain transportation is between laboratory operation；
2) prepared by intestinal flora preparation:
A, fresh excreta is divided into two parts in anaerobism work station, two parts of volume ratios are 2:1, and a transfer that volume is less
The synchronization process into Biohazard Safety Equipment；
B, it is added sterile saline into excrement, and is stirred with glass bar to pulverizing solid manure, then stirred with magnetic stirring apparatus
It mixes mixed liquor for a period of time, forms stable suspension；
C, suspension is first slow transitted through to the filter screen of 40 mesh, removes bulky grain residue, retains filtered fluid A, then filtered fluid A is delayed
The slow filter screen for passing through 80 mesh, further removes particulate matter and swill, retains filtered fluid B；
D, filtered fluid B is dispensed into centrifuge tube, then using centrifuge centrifugation a period of time, removes supernatant, collect precipitating；
E, precipitating is resuspended with sterile saline again, stirs into suspension, and addition sterilizing is sweet in the suspension being stirred
1min is sufficiently stirred after oil；
F, suspension is prepared according to the step of a-e under aerobic conditions, and the suspension prepared under aerobic conditions is transported to
It is mixed in anaerobism work station with the suspension prepared under anaerobic condition, is prepared into intestinal flora liquid；
3) intestinal flora preparation is filling:
After cillin bottle sterilization, the finally obtained intestinal flora liquid of step 2) is poured into cillin bottle fill under anaerobic
Dress, the sealing of blend compounds plug obtain the intestinal flora preparation in a kind of caprophyl transplanting clinical treatment after labelled.
2. the technique of the intestinal flora preparation in caprophyl transplanting clinical treatment according to claim 1, which is characterized in that institute
The temperature for stating cold chain transportation in step 1) is 4 DEG C.
3. the technique of the intestinal flora preparation in caprophyl transplanting clinical treatment according to claim 1, which is characterized in that institute
Stating sterile saline and the volume ratio of excrement in the b step of step 2) is 4:1, sterile physiological in the step e of the step 2)
The volume ratio of salt water and precipitated liquid is 2:1.
4. the technique of the intestinal flora preparation in caprophyl transplanting clinical treatment according to claim 1, which is characterized in that institute
The mixing speed for stating magnetic stirring apparatus in the b step of step 2) is 800-1500rpm, mixing time 5-10min.
5. the technique of the intestinal flora preparation in caprophyl transplanting clinical treatment according to claim 1, which is characterized in that institute
Stating centrifuging temperature in the Step d of step 2) is 4 DEG C, centrifugal speed 20000-24000r/min, centrifugation time 5min.
6. the technique of the intestinal flora preparation in caprophyl transplanting clinical treatment according to claim 1, which is characterized in that institute
Stating sterile glycerol and suspension volume ratio in the step e of step 2) is 1:4-6.
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| CN110836808A (en) * | 2019-11-19 | 2020-02-25 | 张俊美 | Intestinal excrement detection system |
| CN112375705A (en) * | 2020-11-12 | 2021-02-19 | 安徽燕婉健康科技有限公司 | Preparation method and application of intestinal wall transplantation dominant bacteria replacing natural flora |
| WO2021134740A1 (en) * | 2020-01-03 | 2021-07-08 | 四川农业大学 | Tibetan pig fecal microbiota capsule for relieving diarrhea in weaned piglet, and preparation method therefor |
| CN113499350A (en) * | 2021-07-06 | 2021-10-15 | 云南农业大学 | Method for improving transport stress resistance of Kunming dog puppy by using fecal strain transplantation |
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