CN106367329A - High-speed efficient separation device for microorganisms and organic molecules in sample - Google Patents
High-speed efficient separation device for microorganisms and organic molecules in sample Download PDFInfo
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- CN106367329A CN106367329A CN201610874050.1A CN201610874050A CN106367329A CN 106367329 A CN106367329 A CN 106367329A CN 201610874050 A CN201610874050 A CN 201610874050A CN 106367329 A CN106367329 A CN 106367329A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
- C12M47/02—Separating microorganisms from the culture medium; Concentration of biomass
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D33/00—Filters with filtering elements which move during the filtering operation
- B01D33/01—Filters with filtering elements which move during the filtering operation with translationally moving filtering elements, e.g. pistons
- B01D33/03—Filters with filtering elements which move during the filtering operation with translationally moving filtering elements, e.g. pistons with vibrating filter elements
- B01D33/0346—Filters with filtering elements which move during the filtering operation with translationally moving filtering elements, e.g. pistons with vibrating filter elements with flat filtering elements
- B01D33/0353—Filters with filtering elements which move during the filtering operation with translationally moving filtering elements, e.g. pistons with vibrating filter elements with flat filtering elements self-supporting
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D33/00—Filters with filtering elements which move during the filtering operation
- B01D33/35—Filters with filtering elements which move during the filtering operation with multiple filtering elements characterised by their mutual disposition
- B01D33/41—Filters with filtering elements which move during the filtering operation with multiple filtering elements characterised by their mutual disposition in series connection
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/02—Separating microorganisms from their culture media
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- Health & Medical Sciences (AREA)
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- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention provides a high-speed efficient separation device for microorganisms and organic molecules in a sample. The high-speed efficient separation device comprises a box body, and a stirring tank, a filter, a pressure pump, a liquid collection device, a deodorization device and a water storage tank which are arranged in the box body; the stirring tank, the filter, the pressure pump and the liquid collection device are sequentially connected; the outer box body is provided with a first gas inlet, a second gas inlet, a first gas outlet and a second gas outlet; the second gas inlet and the second gas outlet are respectively communicated with the inside of the box body; the first gas inlet is connected with a gas inlet of the stirring tank; a gas outlet of the stirring tank, a gas outlet of the filter and a gas outlet of the liquid collection device are respectively connected with the deodorization device, and a gas outlet of the deodorization device is connected with the first gas outlet; a stirring device is arranged in the stirring tank, and a filter screen is arranged in the filter; the water storage tank is connected with the first gas inlet and a gas inlet of the stirring tank. The separation device is simple in structure, low in cost and convenient to use, and meets actual clinical requirements.
Description
Technical field
The invention belongs to technical field of medical instruments, particularly to microparticle thing in a kind of quick automatic separation biased sample
Device, specifically containing the microorganism in object sample and organic molecule rapidly and efficiently segregation apparatuss.
Background technology
The micro organism quantity containing in human body exceedes more than 10 times of human body own cells, wherein intestinal microflora
For huge, the aspect such as it is developed to nutrient metabolism, human body itself, generation of immunity and disease all plays extremely important work
With.Many results of study all show that the composition of enteric microorganism is closely related with multiple disease incidences, such as intestinal tract disease, obesity,
The diseases such as cholelithiasis, diabetes, cancer, hypertension, hyperlipidemia, or even the mental status of infantile autism, depression and people are all therewith
There is direct contact.In clinical practice, progressively intestinal microbial population is adjusted the important target as the various diseases for the treatment of
Point, the most direct mode of one of which is exactly that healthy human body intestinal canal flora is transplanted in Intestinal Mucosal Injury in Patients Undergoing after treatment,
But the processing procedure obtaining healthy human body intestinal microbial population is a time, the process of environmental condition requirement harshness, effective acquisition intestinal
These target components in road, need to rely on professional equipment to complete.
" caprophyl transplanting " is also referred to as " feces transplanting ", " caprophyl treatment ", " Enterobacter cloaca transplanting " or " transplanting of intestinal Tiny ecosystem ", excrement
Bacterium transplanting is also considered as a kind of transplanting of special organ, diseases related in the invalid infection for the treatment of antibiotic etc, such as
Antibiotic-associated diarrhea, Treatment of Refractory Irritable Bowel Syndrome, immune-related intractable diarrhoea, refractory inflammatory bowel disease, difficulty
Distinguish clostridial infection, food anaphylaxiss, obesity, depression bowel symptoms, fatty liver etc., therapeutic effect is especially pronounced, so far
About 10,000 patients accept caprophyl transplantation treatment in the modern whole world.Human body intestinal canal endoparasitism about 1,000,000,000,000,000 antibacterials, about
500～1000 different species, because the intestinal environment oxygen content that it is survived is low, temperature is 35 DEG C of body temperature to 40
DEG C, therefore most of intestinal bacteria is anaerobic bacteria.Have at present 50%-90% intestinal bacteria can not by artificial culture out,
All limited to the understanding of its function and conditions of existence.In caprophyl transplantation treatment, one of object of transplanting is exactly viable bacteria it is ensured that living
Bacterium implantation rate is to treat successful basic guarantee.The main source obtaining caprophyl is the feces of healthy human body, is contaminated with feces
The solid particles such as food debriss, crude fibre are it is necessary first to carry out filtration treatment.Intestinal microbial population is being obtained from healthy human body feces
In operating process: 1. how to ensure pollution-free；2. avoid anaerobe contact oxygen loss；3. how control operation ambient temperature makes
Obtain thalline loss rate minimum, overall structure keeps constant；4. how quickly to obtain object, the problems such as meet clinical needs be all
Obtain the key technical problems of caprophyl.
Probioticss in intestinal, such as bacillus bifiduss, lactobacilluss etc. can synthesize multiple growth in humans and develop necessary dimension life
Element, such as b vitamin (vitamin b1, b2, b6, b12), vitamin k, nicotinic acid, pantothenic acid etc. are moreover it is possible to be synthesized using protein residue
Essential amino acids.Intestinal microbial population not being host marking, but with human body symbiotic population, metabolism in intestinal for the intestinal microbial population is in a steady stream
Constantly for providing the necessary nutrient substance of human body, therefore gut metabolism also becomes " first metabolism ".Metabolite is to act on people
Each tissue of body and the most direct material of organ, therefore in caprophyl migration process, another object of transplanting is needed by human body
Bacterial metabolism product.Typically organic metabolite stability is poor, unstable under the conditions of high temperature aerobic environment for a long time, is obtaining
Remain a need for solving the problems, such as sample treatment temperature, oxygen concentration and time during donor gut metabolism product.
Content of the invention
Technical problem: in order to solve the defect of prior art, the invention provides microorganism in a kind of sample and organic
Molecule rapidly and efficiently segregation apparatuss.
Technical scheme: the microorganism in a kind of sample that the present invention provides and organic molecule rapidly and efficiently segregation apparatuss, bag
Include casing and the agitator tank in casing, filter, compression pump, fluid collection device, odor removal, water tank, stirring
Tank, filter, compression pump, fluid collection device are sequentially connected；First air inlet, the second air inlet are respectively equipped with described outer case
Mouth, the first gas outlet, the second gas outlet, described second air inlet, the second gas outlet are connected with box house respectively；Described first
Air inlet is connected with the air inlet of agitator tank, the gas outlet of agitator tank, the gas outlet of filter, the gas outlet of fluid collection device
It is connected with odor removal respectively, the gas outlet of odor removal is connected with the first gas outlet；It is provided with agitating device in described agitator tank,
It is provided with drainage screen in filter；Described water tank is connected with the air inlet of the first air inlet and agitator tank respectively.
As improvement, also include sterilizing lamp, described sterilizing lamp is in casing.
Improve as another kind of, also include temperature control equipment, described temperature control equipment is in casing.
Improve as another kind of, also include first gas defecator and second gas defecator, described first gas
Defecator is connected with the first air inlet, the second air inlet respectively, described second gas defecator respectively with the first gas outlet,
Second gas outlet connects.First gas defecator is passed through pure gases or two or more mixed gas；Preferably, the first gas
The gas that body defecator is passed through is other gases beyond oxygen, can create oxygen-free environment.
Improve as another kind of, also include gas-detecting device, described gas-detecting device respectively with the first gas outlet, the
Two gas outlets connect.
Improve as another kind of, described filter is provided with the device making material concussion to be filtered in filter；Preferably,
Described filter is provided with the agitator making filter body vibration, or is provided with the vibration dress making drainage screen vibrate in filter
Put.
Improve as another kind of, in described filter, be provided with multiple filtration net；Drainage screen be arranged in parallel from top to bottom
One group of filter screen, its aperture is sequentially reduced from top to bottom；Or drainage screen is to be obliquely installed one group of filter screen, adjacent screen from top to bottom
Edge is abutted against or is not abutted against, and its aperture is sequentially reduced from top to bottom；Filter screen is flat screen, or middle part concave downward, or
Middle part raises up.
Improve as another kind of, described fluid collection device includes one group of receiving flask being arranged in series, seals receiving flask
The syringe needle for receiving flask series connection in rubber stopper, the sterilization plug of parcel rubber stopper, insertion plug, syringe needle is big transfusion plug bottle
Pin；It is provided with cotton ball soaked in alcohol in sterilization plug.
Improve as another kind of, described odor removal includes air inlet pipe and escape pipe；The gas outlet of water inlet pipe is located at and removes
Smelly device bottom, the gas feed of escape pipe is located at odor removal top.
Present invention also offers a kind of utilization said apparatus rapidly and efficiently separate to the microorganism in sample and organic molecule
Method, comprise the following steps:
(1) first gas defecator is passed through other gases that gas is beyond oxygen, and the second air inlet is by casing
Air is discharged, and the air in agitator tank, filter, fluid collection device, odor removal is discharged by the first air inlet, makes in device
Form oxygen-free environment；
(2) sample to be separated is placed in agitator tank, using water tank, suitable quantity of water is pressed in agitator tank, opens stirring
Device stirs；
(3) slurry in agitator tank enters sucking filtration in filter, and the filtrate containing microorganism and organic molecule is received by liquid
Acquisition means are collected；
Gas is passed through in agitator tank (4) first air inlets, and brings deodorization in odor removal into；Meanwhile, filter and
Gas in fluid collection device is passed through deodorization in odor removal.
Beneficial effect: the segregation apparatuss structure that the present invention provides is simple, with low cost, easy to use it is ensured that separation is whole
The closing of body operating environment is pollution-free, can manufacture anaerobic environment or aerobic environment simultaneously, controls in separation process sample temperature and
Obtain the temperature of bacterium solution eventually, efficiently quickly obtain object within the shortest time, to meet actual clinical needs.
Brief description
Fig. 1 is the structural representation of microorganism in inventive samples and organic molecule rapidly and efficiently segregation apparatuss.
Fig. 2 is the structural representation that in filter, adjacent screen edge is abutted against.
Fig. 3 is the structural representation of the filter screen of middle part concave downward in filter.
Fig. 4 is the structural representation of the filter screen that middle part raises up in filter.
Fig. 5 is the structural representation of fluid collection device.
Specific embodiment
Below the present invention is further illustrated.
Microorganism in sample and organic molecule rapidly and efficiently segregation apparatuss, are shown in Fig. 1, including casing 1 and located at casing
Agitator tank 2 in 1, filter 3, compression pump 4, fluid collection device 5, odor removal 6, water tank 7, sterilizing lamp 8, temperature control
Device 9, first gas defecator 15 and second gas defecator 16, gas-detecting device 17, agitator tank 2, filter 3,
Compression pump 4, fluid collection device 5 are sequentially connected；First air inlet 11, the second air inlet 12, first are respectively equipped with outer case 1
Gas outlet 13, the second gas outlet 14；First gas defecator 15 is connected with the first air inlet 11, the second air inlet 12 respectively,
Second gas defecator 16 is connected with the first gas outlet 13, the second gas outlet 14 respectively, gas-detecting device 17 and the second gas
Body defecator 16 connects, and the second air inlet 12, the second gas outlet 14 are internal with casing 1 respectively to be connected；First air inlet 11 with
The air inlet of agitator tank 2 connects, the gas outlet of agitator tank 2, the gas outlet of filter 3, the gas outlet difference of fluid collection device 5
It is connected with odor removal 6, the gas outlet of odor removal 6 is connected with the first gas outlet 13；It is provided with agitating device 21 in agitator tank 2,
It is provided with drainage screen 31 in filter 3；Water tank 7 is connected with the air inlet of the first air inlet 11 and agitator tank 2 respectively；Sterilizing lamp 8
In casing 1；Temperature control equipment 9 is in casing 1.
Filter 3 is provided with the device making material concussion to be filtered in filter 3；Filter 3 is provided with makes filter 3 whole
The agitator 32 of body vibration, or in filter 3, it is provided with the vibrating device making drainage screen 31 vibrate, all can realize mesh of the present invention
's.
It is provided with multiple filtration net 31 in filter 3；The setting of drainage screen can take various forms, and all can realize the present invention
Purpose, for example: drainage screen 31 is one group of filter screen be arrangeding in parallel from top to bottom, and its aperture is sequentially reduced from top to bottom, sees figure
1；Or drainage screen 31 is to be obliquely installed one group of filter screen from top to bottom, and adjacent screen edge is abutted against to be seen Fig. 2 or be not abutted against, its aperture
It is sequentially reduced from top to bottom；Filter screen 31 is flat screen, or middle part concave downward is shown in Fig. 3, or middle part convex for the sake of scheme
4.
Odor removal 6 includes air inlet pipe 61 and escape pipe 62；The gas outlet of water inlet pipe 61 is located at odor removal 6 bottom,
The gas feed of escape pipe 62 is located at odor removal 6 top.
Fluid collection device 5, is shown in Fig. 5, including the rubber stopper of one group of receiving flask being arranged in series 51, sealing receiving flask 51
52nd, the sterilization plug 54 of parcel rubber stopper 52, the syringe needle 53 for receiving flask 51 series connection inserting in plug 52, syringe needle 53 is defeated greatly
Liquid needle for inserting bottle；It is provided with cotton ball soaked in alcohol in sterilization plug 54；Many benefits are had using big needle for inserting bottle for infusion: first, side
Just change receiving flask；Second, wide material sources, aseptic.
Using said apparatus to the microorganism in sample and organic molecule rapidly and efficiently detached method, step is as follows:
(1) first gas defecator 15 is passed through other gases that gas is beyond oxygen, and the second air inlet 12 is by casing 1
Interior air is discharged, and the air in agitator tank 2, filter 3, fluid collection device 5, odor removal 6 is arranged by the first air inlet 11
Go out, make to be formed oxygen-free environment in device；
(2) sample to be separated is placed in agitator tank 2, using water tank 7, suitable quantity of water is pressed in agitator tank 2, opens
Agitating device 21 stirs；
(3) slurry in agitator tank 2 enters sucking filtration in filter 3, and the filtrate containing microorganism and organic molecule passes through liquid
Collection device 5 is collected；
Gas is passed through in agitator tank 2 (4) first air inlets 11, and brings deodorization in odor removal 6 into；Meanwhile, filter 3
And the gas in fluid collection device 5 is passed through deodorization in odor removal 6.
Claims (10)
1. the microorganism in a kind of sample and organic molecule rapidly and efficiently segregation apparatuss it is characterised in that: include casing (1), with
And the agitator tank (2) in casing (1), filter (3), compression pump (4), fluid collection device (5), odor removal (6), storage
Water pot (7), agitator tank (2), filter (3), compression pump (4), fluid collection device (5) are sequentially connected；On described outer case (1)
It is respectively equipped with the first air inlet (11), the second air inlet (12), the first gas outlet (13), the second gas outlet (14), described second
Air inlet (12), the second gas outlet (14) connect with inside casing (1) respectively；Described first air inlet (11) and agitator tank (2)
Air inlet connect, the gas outlet of agitator tank (2), the gas outlet of filter (3), fluid collection device (5) gas outlet respectively
It is connected with odor removal (6), the gas outlet of odor removal (6) is connected with the first gas outlet (13)；It is provided with described agitator tank (2)
Agitating device (21), is provided with drainage screen (31) in filter (3)；Described water tank (7) respectively with the first air inlet (11) and stirring
The air inlet mixing tank (2) connects.
2. the microorganism in a kind of sample according to claim 1 and organic molecule rapidly and efficiently segregation apparatuss, its feature
It is: also include sterilizing lamp (8), described sterilizing lamp (8) is in casing (1).
3. the microorganism in a kind of sample according to claim 1 and organic molecule rapidly and efficiently segregation apparatuss, its feature
It is: also include temperature control equipment (9), described temperature control equipment (9) is in casing (1).
4. the microorganism in a kind of sample according to claim 1 and organic molecule rapidly and efficiently segregation apparatuss, its feature
It is: also include first gas defecator (15) and second gas defecator (16), described first gas defecator
(15) it is connected with the first air inlet (11), the second air inlet (12) respectively, described second gas defecator (16) is respectively with
One gas outlet (13), the second gas outlet (14) connect；First gas defecator (15) is passed through pure gases or two or more
Mixed gas；Preferably, the gas that first gas defecator (15) is passed through is other gases beyond oxygen.
5. the microorganism in a kind of sample according to claim 1 and organic molecule rapidly and efficiently segregation apparatuss, its feature
Be: also include gas-detecting device (17), described gas-detecting device (17) respectively with the first gas outlet (13), second give vent to anger
Mouth (14) connects.
6. the microorganism in a kind of sample according to claim 1 and organic molecule rapidly and efficiently segregation apparatuss, its feature
It is: described filter (3) is provided with the device making material concussion to be filtered in filter (3)；Preferably, described filter
(3) being provided with makes the agitator (32) of filter (3) body vibration, or be provided with filter (3) make drainage screen (31) vibrate
Vibrating device.
7. the microorganism in a kind of sample according to claim 1 and organic molecule rapidly and efficiently segregation apparatuss, its feature
It is: in described filter (3), be provided with multiple filtration net (31)；Drainage screen (31) is one group of filter be arrangeding in parallel from top to bottom
Net, its aperture is sequentially reduced from top to bottom；Or drainage screen (31) is to be obliquely installed one group of filter screen, adjacent screen side from top to bottom
Edge is abutted against or is not abutted against, and its aperture is sequentially reduced from top to bottom；Drainage screen (31) is flat screen, or middle part concave downward,
Or middle part raises up.
8. the microorganism in a kind of sample according to claim 1 and organic molecule rapidly and efficiently segregation apparatuss, its feature
It is: described fluid collection device (5) includes one group of receiving flask being arranged in series (51), the rubber stopper of sealing receiving flask (51)
(52), the sterilization plug (54) of parcel rubber stopper (52), the syringe needle (53) connected for receiving flask (51) inserting in plug (52),
Syringe needle (53) is big needle for inserting bottle for infusion；It is provided with cotton ball soaked in alcohol in sterilization plug (54).
9. the microorganism in a kind of sample according to claim 1 and organic molecule rapidly and efficiently segregation apparatuss, its feature
It is: described odor removal (6) includes air inlet pipe (61) and escape pipe (62)；The gas outlet of water inlet pipe (61) is located at deodorization and fills
Put (6) bottom, the gas feed of escape pipe (62) is located at odor removal (6) top.
10. a kind of device of utilization claim 1 to 9 is to the microorganism in sample and organic molecule rapidly and efficiently detached side
Method it is characterised in that: comprise the following steps:
(1) first gas defecator (15) is passed through other gases that gas is beyond oxygen, and the second air inlet (12) is by casing
(1) air in is discharged, and the first air inlet (11) is by agitator tank (2), filter (3), fluid collection device (5), odor removal
(6) air in is discharged, and makes to be formed oxygen-free environment in device；
(2) sample to be separated is placed in agitator tank (2), using water tank (7), suitable quantity of water is pressed in agitator tank (2), opens
Open agitating device (21) stirring；
(3) slurry in agitator tank (2) enters sucking filtration in filter (3), and the filtrate containing microorganism and organic molecule passes through liquid
Collection device (5) is collected；
Gas is passed through in agitator tank (2) (4) first air inlets (11), and brings deodorization in odor removal (6) into；Meanwhile, filter
Gas in device (3) and fluid collection device (5) is passed through deodorization in odor removal (6).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610874050.1A CN106367329A (en) | 2016-09-30 | 2016-09-30 | High-speed efficient separation device for microorganisms and organic molecules in sample |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610874050.1A CN106367329A (en) | 2016-09-30 | 2016-09-30 | High-speed efficient separation device for microorganisms and organic molecules in sample |
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| Publication Number | Publication Date |
|---|---|
| CN106367329A true CN106367329A (en) | 2017-02-01 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610874050.1A Pending CN106367329A (en) | 2016-09-30 | 2016-09-30 | High-speed efficient separation device for microorganisms and organic molecules in sample |
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| CN (1) | CN106367329A (en) |
Cited By (2)
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|---|---|---|---|---|
| CN109517727A (en) * | 2019-01-17 | 2019-03-26 | 上海宝藤生物医药科技股份有限公司 | A kind of separator and separation method of enteric microorganism |
| CN110564609A (en) * | 2019-09-12 | 2019-12-13 | 上海市第十人民医院 | Microorganism separation processing apparatus |
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