CN113680542B

CN113680542B - Adipose-derived stem cell collection device

Info

Publication number: CN113680542B
Application number: CN202110981085.6A
Authority: CN
Inventors: 黄勇
Original assignee: Hunan Tongnian Holdings Co ltd
Current assignee: Hunan Tongnian Holdings Co ltd
Priority date: 2021-08-25
Filing date: 2021-08-25
Publication date: 2023-12-12
Anticipated expiration: 2041-08-25
Also published as: CN113680542A

Abstract

The application discloses a fat stem cell collecting device, which relates to the technical field of stem cells and comprises a brake box frame, wherein a limiting clamping plate is arranged at the top of the brake box frame, a pumping and injecting assembly is slidably connected with the limiting clamping plate, an anti-slip type material guiding hose is connected to the bottom of the pumping and injecting assembly, one end of the anti-slip type material guiding hose is connected with a pumping and injecting transition box, one end of the brake box frame is provided with a conveying sterilizing cylinder which is communicated with the conveying sterilizing cylinder, one side of the brake box frame is provided with a detachable operation folded plate, one side of the limiting clamping plate is provided with annular clamping grooves at equal intervals, and the side wall of the inner side of each annular clamping groove is provided with limiting clamping grooves; not only can avoid medical personnel to contact with the syringe of extraction or injection material with matter centrifuge tube for a long time, can effectually avoid bacterium and virus to get into the inside adipose tissue of centrifuging tube, simultaneously, when obtaining stem cell group with adipose tissue centrifugation, can prevent that the centrifuging tube from being thrown away by centrifugal force, also can avoid the centrifuging tube to take place to collide with and cause the damage.

Description

Adipose-derived stem cell collection device

Technical Field

The application relates to the technical field of stem cell collecting devices, in particular to a fat stem cell collecting device.

Background

The adipose tissue has abundant reserves in human bodies, a large amount of adipose stem cells obtained from the adipose tissue through liposuction have self-renewing proliferation and multidirectional differentiation potential, can differentiate into adipose cells, cartilage cells, muscle cells, osteoblasts, nerve cells, glial cells and islet cells, can secrete various pro-angiogenic factors and anti-apoptosis factors to resist inflammation and oxidation, can resist the injury of antioxidant free radicals, and is hopeful to become a stem cell source for repairing damaged tissues and organs;

when adipose tissue stem cells are extracted, adipose tissue is required to be digested and decomposed, and then the obtained adipose tissue stem cell mass is subjected to centrifugation and buffer solution injection, when digestive solution is injected into adipose tissue, digested substances are extracted and buffer solution is injected into adipose tissue, one hand of a medical staff is used for holding a centrifuge tube filled with adipose tissue, and then the operation is completed through the syringe;

when the centrifugal device is used for centrifugal separation of adipose tissues, the centrifugal tube is obliquely arranged inside the centrifugal barrel, so that the centrifugal tube is easily thrown out by the centrifugal force, and after the centrifugal tube is arranged inside the centrifugal barrel, collision is easily generated during centrifugation, and therefore the centrifugal tube is damaged.

Disclosure of Invention

Therefore, in order to solve the above-mentioned shortcomings, the present application provides a adipose-derived stem cell collection device, in which, when stem cells are extracted, not only can the medical staff be prevented from contacting with a syringe for extracting or injecting substances for a long time, but also bacteria and viruses can be effectively prevented from entering into adipose tissues in a centrifuge tube, and in addition, when digestive fluid is injected into adipose tissues, the digested substances are extracted and buffer fluid is injected into adipose tissues, the injection is completed without using a syringe by one hand of the medical staff, thus reducing the burden of the medical staff; meanwhile, when the adipose tissues are centrifuged to obtain stem cell clusters, the centrifugal tubes can be prevented from being thrown out by centrifugal force, and damage to the centrifugal tubes caused by collision can be avoided.

The application is realized in such a way, and a fat stem cell collecting device is constructed, and the fat stem cell collecting device comprises a brake box frame, wherein a limit clamping plate is arranged at the top of the brake box frame, a drawing and injecting assembly is connected to the limit clamping plate in a sliding manner, an anti-slip type material guiding hose is connected to the bottom of the drawing and injecting assembly, one end of the anti-slip type material guiding hose is connected with a drawing and injecting transition box, one end of the brake box frame is provided with a conveying sterilizing cylinder which is communicated with the conveying sterilizing cylinder, a detachable operation folded plate is arranged on one side of the brake box frame, annular clamping grooves are formed in one side of the limit clamping plate at equal intervals, and limit clamping grooves are formed in the side wall of the inner side of the annular clamping grooves.

Further, the inner side of the conveying disinfection cylinder is also connected with a cell separator, the cell separator comprises a transparent centrifuge tube, a T-shaped supporting table, a threaded rotary sleeve, a threaded sleeve, a plug cover, a square limiting groove, a square base and a clamping rod, the top of the T-shaped supporting table is connected with the transparent centrifuge tube, the threaded rotary sleeve is connected to the outer side of the T-shaped supporting table through a bearing, the top of the T-shaped supporting table is provided with a square limiting hole, the threaded sleeve is connected to the outer side of the threaded rotary sleeve through threads, the bottom of the clamping rod is hinged to the edge-along position of the top of the threaded sleeve, the plug cover is embedded into the top of the transparent centrifuge tube, the bottom of the transparent centrifuge tube is the square base, and the square limiting groove is formed in four sides of the square base.

Further, the clamping rod comprises a bottom rod, a clamping rod and a limiting clamping block, the bottom of the clamping rod is hinged to the top of the bottom rod, and the limiting clamping block is arranged on one side of the top of the clamping rod.

Further, the outer edge of the top of the threaded rotary sleeve is provided with a limiting edge plate, and the outer edge of the bottom of the threaded rotary sleeve is provided with a rotary plate.

Further, take out notes transition box includes interior storehouse, turn over the door, second ultraviolet lamp, exit tube logical groove, press briquetting, spacing pipe, spacing spout, spring and clamp frame, interior storehouse outside articulates there is turns over the door, interior storehouse inside one side is equipped with second ultraviolet lamp, exit tube logical groove is seted up in interior storehouse bottom, spacing spout is seted up in interior inside opposite side of storehouse, and link up interior inside opposite side curb plate of storehouse, press briquetting one side sliding connection in spacing spout inboard, press briquetting bottom is connected with the spring, press briquetting sliding connection is equipped with clamp frame in the part of spacing spout, spacing pipe has been seted up at interior storehouse top.

Further, the bottom of the pressing block is a pressing plate, one side of the top of the pressing block is a sliding block, the inner side of the clamping frame is provided with a clamping groove, and the inner side of the clamping groove is provided with a magnetic sheet.

Further, take out and annotate the subassembly and include hydraulic stem, coupling assembling, injection tube, spacing ring plate, top connector, fastening spacing ring, connecting thread A and oblique pipe, the hydraulic stem bottom is connected in the injection tube top through coupling assembling, and the injection tube bottom is located to the oblique pipe, and the injection tube bottom is located to spacing ring plate is located to the injection tube bottom and is followed the position outward, and the top connector is located injection tube piston handle top, and the top connector outside is equipped with connecting thread A, and oblique pipe bottom is equipped with the fastening spacing ring.

Further, the connection assembly electricity top is equipped with the top ring, and top ring bottom is equipped with end connector, and connecting thread B has been seted up in the end connector outside, and end connector outside is connected with the thread bush through connecting thread B, and spacing ring plate both sides top is equipped with rubber cushion.

Further, the anti-slip type guide hose is sequentially provided with a pumping and injecting tube head, a connecting rubber block, a clamping head, an infusion speed regulator and a clamping head, wherein the pumping and injecting tube head and the clamping head are respectively arranged at two ends of the anti-slip type guide hose, an armature ring is nested at the outer side of the connecting rubber block, a first elastic leather sheath is arranged at the outer side of the clamping head, and a second elastic leather sheath is arranged at the outer side of the clamping head.

Further, the detachable operation folded plate top both sides are equipped with the connection flange, and detachable operation folded plate riser middle part is equipped with spacing turn-ups section of thick bamboo, and spacing turn-ups section of thick bamboo one end is connected with prevents contact oversleeve, prevents that contact oversleeve one end is equipped with the hollow layer, and the hollow in situ side is equipped with the elastic cord.

Further, the brake box frame comprises an industrial tablet personal computer, a hydraulic system, a cell separation bin, a supporting connecting plate and a rubber clamping plate, wherein the supporting connecting plate is arranged on the outer sides of two ends of the top of the cell separation bin, the industrial tablet personal computer is arranged at one end of the cell separation bin, the hydraulic system is arranged on one side of the cell separation bin, and the rubber clamping plate is nested on the outer side of the supporting connecting plate.

Further, the conveying disinfection section of thick bamboo includes first ultraviolet lamp, electronic slide rail, sliding seat, rotating electrical machines, guard gate and curtain, and the sliding seat is installed at electronic slide rail panel top, and the rotating electrical machines is installed to the sliding seat inboard, and the guard gate articulates in conveying disinfection section of thick bamboo one end, and first ultraviolet lamp installs in conveying disinfection section of thick bamboo both sides, and the curtain is installed and is leaned on the inboard in conveying disinfection section of thick bamboo and brake box frame intercommunication department and guard gate installation department lateral part.

Compared with the prior art, the application has the beneficial effects that:

advantage 1: according to the application, when the fat stem cells are extracted to inject or extract substances into the centrifuge tube, one hand of a medical staff stretches into the anti-contact sleeve to enter the cell separation bin to press the pressing block for assistance, and then the other hand of the medical staff is used for injecting or extracting substances into the centrifuge tube by controlling the industrial tablet computer to cooperate with the pumping and injecting assembly, the hydraulic system and the anti-slip material guide hose, so that the medical staff is effectively prevented from contacting with the syringe for extracting or injecting substances for a long time with the substance centrifuge tube, and bacteria and viruses can be effectively prevented from entering fat tissues in the centrifuge tube.

Advantage 2: when medical staff injects digestive juice into adipose tissues, extracts digested substances and injects buffer solution, the injection is completed by controlling the industrial tablet computer to cooperate with the injection drawing component, the hydraulic system and the anti-slip guide hose, and the medical staff does not need to use a syringe with one hand, so that the burden of the medical staff is greatly reduced.

Advantage 3: according to the application, the centrifugal tube is a transparent centrifugal tube, the transparent centrifugal tube is placed on the inner side of a square limiting hole at the top of the T-shaped supporting table through the square limiting hole, the square base and the transparent centrifugal tube are limited, then the rotary plate is stirred by the other hand, the whole threaded rotary sleeve is driven to rotate by the rotary plate, the threaded sleeve is pushed to slide on the outer side of the threaded rotary sleeve, when the threaded sleeve slides along the plate direction in a limiting manner, the threaded sleeve drives the bottom rod to move to the top, and simultaneously, the bottom rod is pushed to push the bottom of the clamping rod to prop open outwards, so that the top of the clamping rod drives the limiting clamping block to gather towards the square limiting groove of the square base, at the moment, the tops of four groups of clamping rods drive the limiting clamping block to gather towards the square limiting groove of the square base until the limiting clamping block is embedded into the inner side of the square limiting groove, the square base is rapidly clamped, meanwhile, the transparent centrifugal tube is also clamped, the transparent centrifugal tube is prevented from being thrown out by centrifugal force, and damage caused by collision of the transparent centrifugal tube can be avoided.

Drawings

FIG. 1 is a schematic diagram of the adipose-derived stem cell collection device of the present application;

FIG. 2 is a front view of the present application;

FIG. 3 is a cross-sectional view of a delivery sterilization drum of the present application;

FIG. 4 is a schematic view of the structure of the rotary electric machine of the present application;

FIG. 5 is a schematic diagram of the structure of a cell separation cartridge according to the present application;

FIG. 6 is an enlarged view of a portion of the support adapter plate of FIG. 5 in accordance with the present application;

FIG. 7 is a schematic view of a detachable operating flap of the present application;

FIG. 8 is a schematic view of the anti-contact sleeve of the present application;

fig. 9 is an enlarged view of a portion of the application at M of fig. 8;

FIG. 10 is a schematic diagram of a pumping and injecting assembly according to the present application;

FIG. 11 is a schematic view of the structure of the syringe of the present application;

FIG. 12 is an enlarged view of a portion of the connection assembly of FIG. 10 in accordance with the present application;

FIG. 13 is a schematic view of the structure of the connecting thread B of the present application;

FIG. 14 is an enlarged view of a portion of the top connector of FIG. 11 in accordance with the present application;

FIG. 15 is an enlarged view of a portion of the stop collar plate of FIG. 11 in accordance with the present application;

FIG. 16 is a schematic view of a spacing clip panel structure of the present application;

FIG. 17 is an enlarged view of a portion of the annular slot of FIG. 16 in accordance with the present application;

FIG. 18 is a schematic view of an anti-slip type guide hose according to the present application;

FIG. 19 is an enlarged view of a portion of the connecting rubber block of FIG. 18 in accordance with the present application;

FIG. 20 is an enlarged view of a portion of the chucking head of FIG. 18 in accordance with the present application;

FIG. 21 is an enlarged view of a portion of the chuck of FIG. 18 according to the present application;

FIG. 22 is a schematic diagram of a cell separator according to the present application;

FIG. 23 is a schematic view of the T-shaped support table structure of the present application;

FIG. 24 is a schematic view of the structure of the threaded rotary sleeve of the present application;

FIG. 25 is a schematic view of a clamping lever structure of the present application;

FIG. 26 is an enlarged view of a portion of the inner cartridge of FIG. 3 in accordance with the present application;

FIG. 27 is a side cross-sectional view of the inner cartridge of the present application;

FIG. 28 is a top view of the limit chute of the present application;

FIG. 29 is a schematic view of a pressing block according to the present application;

FIG. 30 is a schematic view of a frame structure of the present application.

In the figure: the brake box frame 1, the conveying disinfection cylinder 2, the detachable operation folded plate 3, the drawing and injecting assembly 4, the limit clamping plate 5, the anti-slip type guide hose 6, the cell separator 7, the drawing and injecting transition box 8, the industrial tablet computer 11, the hydraulic system 12, the cell separation bin 13, the supporting plate 14, the rubber clamping plate 15, the first ultraviolet lamp 21, the electric sliding rail 22, the sliding seat 23, the rotating motor 24, the protective door 25, the curtain 26, the connecting convex plate 31, the anti-contact sleeve 32, the limit flanging cylinder 33, the hollow layer 34, the elastic band 35, the hydraulic rod 41, the connecting assembly 42, the injection cylinder 43, the ring plate 44, the top connector 45, the fastening limiting ring 46, the connecting screw thread A47, the inclined guide pipe 48, the top ring 421, the connecting screw thread B422, the screw sleeve 423 bottom connector 424, rubber cushion 441, annular clamping groove 51, limit clamping groove 52, suction tube head 61, connection rubber block 62, clamping head 63, transfusion speed regulator 64, clamping head 65, armature ring 66, elastic leather sheath 67, elastic leather sheath 68, transparent centrifuge tube 71, T-shaped support table 72, threaded rotating sleeve 73, threaded sleeve 74, plug cover 75, square limit groove 76, square base 77, clamping rod 78, square limit hole 721, rotating plate 731, limit edge plate 732, bottom rod 781, clamping rod 782, limit clamping block 783, inner bin 81, turnover door 82, second ultraviolet lamp 83, exit tube through groove 84, pressing block 85, limit tube 86, limit chute 87, spring 88, clamping frame 89, slider 851, pressing plate 852, clamping groove 891 and magnetic sheet 892.

Detailed Description

The following detailed description of the present application, taken in conjunction with the accompanying drawings, will clearly and completely describe the technical solutions of embodiments of the present application, and it is evident that the described embodiments are only some embodiments of the present application, not all embodiments. All other embodiments, based on the embodiments of the application, which a person of ordinary skill in the art would obtain without inventive faculty, are within the scope of the application; furthermore, the terms "first," "second," "third," "upper, lower, left, right," and the like are used for descriptive purposes only and are not to be construed as indicating or implying relative importance. Meanwhile, in the description of the present application, unless explicitly stated and defined otherwise, the terms "connected", "connected" and "connected" should be interpreted broadly, and for example, may be fixedly connected, detachably connected, or integrally connected; the mechanical connection and the electrical connection can be adopted; can be directly connected or indirectly connected through an intermediate medium. It will be understood by those of ordinary skill in the art that the specific meaning of the terms set forth above in this application will be understood by those of ordinary skill in the art, unless otherwise defined, that all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this application pertains.

The technical scheme of the application is further elaborated by combining the drawings and the specific embodiments of the specification, the fat stem cell collecting device provided by the embodiment of the application comprises a brake box frame 1, wherein a limit clamping plate 5 is arranged at the top of the brake box frame 1, a pumping and injecting assembly 4 is slidably connected to the limit clamping plate 5, an anti-slip type material guiding hose 6 is connected to the bottom of the pumping and injecting assembly 4, a pumping and injecting transition box 8 is connected to one end of the anti-slip type material guiding hose 6, a conveying sterilizing cylinder 2 is arranged at one end of the brake box frame 1 and communicated with the conveying sterilizing cylinder, a detachable operation folded plate 3 is arranged at one side of the brake box frame 1, annular clamping grooves 51 are formed in one side of the limit clamping plate 5 at equal intervals, and limit clamping grooves 52 are formed in the inner side walls of the annular clamping grooves 51.

Referring to fig. 3 and fig. 22-23, the inner side of the conveying sterilizing cylinder 2 is further connected with a cell separator 7, the cell separator 7 comprises a transparent centrifuge tube 71, a T-shaped supporting table 72, a threaded rotary sleeve 73, a threaded sleeve 74, a plug cover 75, a square limiting groove 76, a square base 77 and a clamping rod 78, the top of the T-shaped supporting table 72 is connected with the transparent centrifuge tube 71, the threaded rotary sleeve 73 is connected with the outer side of the T-shaped supporting table 72 through a bearing, the top of the T-shaped supporting table 72 is provided with a square limiting hole 721, the threaded sleeve 74 is connected with the outer side of the threaded rotary sleeve 73 through threads, the bottom of the clamping rod 78 is hinged to the edge position of the top of the threaded sleeve 74, the plug cover 75 is embedded into the top of the transparent centrifuge tube 71, the bottom of the transparent centrifuge tube 71 is provided with the square base 77, and four sides of the square base 77 are provided with the square limiting grooves 76;

the transparent centrifuge tube 71 is used for containing a proper amount of adipose tissues, the plug cover 75 is used for preventing the adipose tissues from spilling out of the transparent centrifuge tube 71, when the transparent centrifuge tube 71 is placed at the top of the T-shaped supporting table 72 through the square base 77, the square base 77 is limited through the square limiting holes 721, and the clamping rods 78 and the square limiting grooves 76 are respectively four groups and correspond to each other.

Referring to fig. 25, the clamping rod 78 includes a bottom rod 781, a clamping rod 782 and a limiting clamping block 783, wherein the bottom of the clamping rod 782 is hinged to the top of the bottom rod 781, and the limiting clamping block 783 is arranged on one side of the top of the clamping rod 782;

one side of the clamping rod 782 is hinged with the top plate edge of the top of the T-shaped supporting table 72 near the middle, the bottom of the bottom rod 781 is hinged with the outer side of the top of the screw sleeve 74, and the limiting clamping block 783 is matched with the square limiting groove 76.

Referring to fig. 24, a limiting plate 732 is disposed on the top outer edge of the screw rotary sleeve 73, and a rotating plate 731 is disposed on the bottom outer edge of the screw rotary sleeve 73;

the limit edge plate 732 prevents the screw sleeve 74 from sliding out of the screw rotating sleeve 73 when sliding outside the screw rotating sleeve 73, and the narrow edge of the rotating plate 731 is provided with a convex angle, so that the screw rotating sleeve is convenient for users to stir.

Referring to fig. 3 and fig. 26-29, the injection transition box 8 includes an inner bin 81, a flip door 82, a second ultraviolet lamp 83, an exit tube through slot 84, a pressing block 85, a limiting tube 86, a limiting chute 87, a spring 88 and a clamping frame 89, wherein the flip door 82 is hinged to the outer side of the inner bin 81, the second ultraviolet lamp 83 is arranged on one side of the inner bin 81, the exit tube through slot 84 is arranged at the bottom of the inner bin 81, the limiting chute 87 is arranged on the other side of the inner bin 81 and penetrates through the side plate of the other side of the inner bin 81, one side of the pressing block 85 is slidably connected to the inner side of the limiting chute 87, the spring 88 is connected to the bottom of the pressing block 85, the clamping frame 89 is arranged on the part of the pressing block 85 slidably connected to the limiting chute 87, and the limiting tube 86 is arranged at the top of the inner bin 81;

the drawing and injecting transition box 8 has four groups, and four groups draw and injecting transition box 8 structure principle is unanimous, press from both sides the frame 89 and the exit tube leads to groove 84 on same perpendicular line, pass spacing pipe 86 with anti-skidding guide hose 6 through drawing and injecting tube head 61, make chucking head 63 card will draw and annotate tube head 61 fixed in spacing pipe 86 inboard, simultaneously with connecting rubber piece 62 card in press from both sides the frame 89 inboard, the user is through pressing down the piece 85 and oppression spring 88, press the piece 85 and slide in spacing spout 87 this moment, drive drawing and injecting tube head 61 decline through pressing from both sides the frame 89 and pass exit tube leads to groove 84 and get into transparent centrifuging tube 71 and draw or inject the tissue solution, second ultraviolet lamp 83 has four groups, and four groups of second ultraviolet lamp 83 are parallelly connected, four groups of second ultraviolet lamp 83 connect external power source simultaneously, open and close through industry panel computer 11 control power, can disinfect for drawing and injecting tube head 61 fixed in drawing and injecting into the transition box 8 inside through second ultraviolet lamp 83.

Referring to fig. 30, the bottom of the pressing block 85 is a pressing plate 852, one side of the top of the pressing block 85 is a sliding block 851, a clamping slot 891 is provided on the inner side of the clamping frame 89, and a magnetic sheet 892 is provided on the inner side of the clamping slot 891;

when the connection rubber block 62 is clamped inside the clamping frame 89, the armature ring 66 is clamped inside the clamping groove 891 to be in contact with the magnetic sheet 892, and the armature ring 66 is attracted by the magnetic sheet 892 to prevent the connection rubber block 62 from sliding off.

Referring to fig. 10-11 and fig. 14-15, the drawing and injecting assembly 4 includes a hydraulic rod 41, a connecting assembly 42, a syringe 43, a limiting ring plate 44, a top connector 45, a fastening limiting ring 46, a connecting thread a47 and an inclined guide tube 48, wherein the bottom of the hydraulic rod 41 is connected to the top of the syringe 43 through the connecting assembly 42, the inclined guide tube 48 is arranged at the bottom of the syringe 43, the limiting ring plate 44 is arranged at the outer edge of the bottom of the syringe 43, the top connector 45 is arranged at the top of the piston handle of the syringe 43, the connecting thread a47 is arranged at the outer side of the top connector 45, and the fastening limiting ring 46 is arranged at the bottom of the inclined guide tube 48;

the pumping and injecting assembly 4 has four groups, four groups of pumping and injecting assemblies 4 are consistent in structure, two groups of pumping and injecting assemblies 4 in the middle among four groups of pumping and injecting assemblies 4 are responsible for pumping solution or other impurities which are not needed in the transparent centrifuge tube 71, in the pumping and injecting assemblies 4 of two groups close to the end of the industrial tablet personal computer 11, the injection cylinder 43 in the pumping and injecting assembly 4 of the other group is used for containing digestion solution, buffer solution is convenient for resuspension of fatty liver cells obtained by centrifugation, when the pumping and injecting assemblies 4 of two groups close to the outer side are used for containing needed solution, the anti-slip guide hose 6 is needed to be connected to the bottom of the inclined guide tube 48, the piston handle of the injection cylinder 43 is pulled to extract the needed solution through the hydraulic rod 41, the hydraulic rod 41 is connected with the hydraulic system 12 through the hydraulic oil tube, the hydraulic system 12 is electrically connected with the industrial tablet personal computer 11, the injection cylinder 43 is clamped into the inner side of the annular clamping groove 51, the limiting ring plate 44 is embedded into the limiting clamping groove 52, and the rubber cushion 441 is extruded simultaneously.

Referring to fig. 12-13, the top of the connecting assembly 42 is provided with a top ring 421, the bottom of the top ring 421 is provided with a bottom connector 424, the outer side of the bottom connector 424 is provided with a connecting thread B422, the outer side of the bottom connector 424 is connected with a thread sleeve 423 through the connecting thread B422, and the tops of two sides of the limiting ring plate 44 are provided with rubber cushions 441;

the connecting screw thread B422 is connected with the connecting screw thread A47 in an adapting way, when the injection tube 43 slides to the inner end of the annular clamping groove 51, the bottom connector 424 is aligned with the top connector 45, meanwhile, the protruding part of the top connector 45 is embedded into the concave part of the bottom connector 424, the protruding part of the bottom connector 424 is embedded into the concave part of the top connector 45, then the screw thread sleeve 423 is rotated, the screw thread sleeve 423 slides to the top connector 45 through the connecting screw thread B422, the screw thread sleeve 423 covers the joint of the bottom connector 424 and the top connector 45, and when the hydraulic rod 41 pulls or pushes the piston handle of the injection tube 43, the connecting pressure of the screw thread sleeve 423 is reduced through the mutual embedding and clamping of the bottom connector 424 and the top connector 45.

Referring to fig. 18-21, the anti-slip type material guiding hose 6 is sequentially provided with a pumping and injecting tube head 61, a connecting rubber block 62, a clamping head 63, an infusion speed regulator 64 and a clamping head 65, wherein the pumping and injecting tube head 61 and the clamping head 65 are respectively arranged at two ends of the anti-slip type material guiding hose 6, an armature ring 66 is nested at the outer side of the connecting rubber block 62, a first elastic leather sheath 67 is arranged at the outer side of the clamping head 63, and a second elastic leather sheath 68 is arranged at the outer side of the clamping head 65;

the antiskid guide hose 6 has four groups, when chucking head 63 card is inside spacing pipe 86, nest in spacing pipe 86 outside through first elastic leather sheath 67, can prevent that antiskid guide hose 6 from entering the part in interior storehouse 81 and drive antiskid guide hose 6 other parts and slide into interior storehouse 81 inside, simultaneously, with the inboard of dop 65 embedding inclined duct 48 bottom, nest No. two elastic leather sheath 68 in the fastening spacing ring 46 outside for No. two elastic leather sheath 68 wraps up the chucking with fastening spacing ring 46, can effectually prevent antiskid guide hose 6 from follow inclined duct 48 bottom landing, infusion speed regulator 64 is unanimous with the infusion velocity of flow regulator principle.

Referring to fig. 7-9, two sides of the top of the detachable operation folded plate 3 are provided with a connecting convex plate 31, the middle of the vertical plate of the detachable operation folded plate 3 is provided with a limit flanging barrel 33, one end of the limit flanging barrel 33 is connected with a contact-preventing oversleeve 32, one end of the contact-preventing oversleeve 32 is provided with a hollow layer 34, and the inner side of the hollow layer 34 is provided with an elastic band 35;

the detachable operation folded plate 3 is a transparent plate, the detachable operation folded plate 3 is nested outside the cell separation bin 13, the connection convex plate 31 is covered at the top of the supporting connection plate 14, then the rubber clamping plate 15 is clamped outside the connection convex plate 31 and the supporting connection plate 14, the detachable operation folded plate 3 is fixed outside the cell separation bin 13, then the glove end of the anti-contact sleeve 32 is sent into the cell separation bin 13 through the limit flanging barrel 33, the other end of the anti-contact sleeve 32 is nested at the flanging position of the limit flanging barrel 33 through the elastic band 35, the anti-contact sleeve 32 is fixed, and meanwhile, the anti-contact sleeve 32 is conveniently replaced and cleaned by taking out the elastic band 35 at the other end of the anti-contact sleeve 32 from the flanging position of the limit flanging barrel 33.

Referring to fig. 1-3 and fig. 5-6, the brake box frame 1 includes an industrial tablet computer 11, a hydraulic system 12, a cell separation bin 13, a supporting connection plate 14 and a rubber clamping plate 15, wherein the supporting connection plate 14 is arranged at the outer sides of two ends of the top of the cell separation bin 13, the industrial tablet computer 11 is arranged at one end of the cell separation bin 13, the hydraulic system 12 is arranged at one side of the cell separation bin 13, and the rubber clamping plate 15 is nested at the outer side of the supporting connection plate 14;

the four groups of hydraulic systems 12 are respectively connected with the hydraulic rods 41 of the four groups of pumping and injecting assemblies 4 through hydraulic oil pipes, and the four groups of hydraulic systems 12 are respectively and electrically connected with the industrial tablet personal computer 11 and are independently controlled by the industrial tablet personal computer 11.

Referring to fig. 1-4, the transmission sterilizing cylinder 2 includes a first ultraviolet lamp 21, an electric slide rail 22, a slide seat 23, a rotating motor 24, a protective door 25 and a curtain 26, wherein the slide seat 23 is installed at the top of a slide rail panel of the electric slide rail 22, the rotating motor 24 is installed at the inner side of the slide seat 23, the protective door 25 is hinged at one end of the transmission sterilizing cylinder 2, the first ultraviolet lamp 21 is installed at two sides of the transmission sterilizing cylinder 2, and the curtain 26 is installed at the position where the transmission sterilizing cylinder 2 is communicated with the brake box frame 1 and the side of the installation position of the protective door 25 is located at the inner side;

the electric slide rail 22 is electrically connected with the industrial tablet computer 11 and is controlled by the industrial tablet computer 11, the electric slide rail 22 is controlled by the industrial tablet computer 11 to drive the slide rail panel to slide, the first ultraviolet lamp 21 is connected with an external power supply, the electric power is controlled by the industrial tablet computer 11 to be turned on or off, the output shaft of the rotating motor 24 passes through the top plate of the inner cavity of the sliding seat 23 through a bearing, the passing part of the electric power is fixedly connected to the bottom of the T-shaped supporting table 72, meanwhile, the rotating motor 24 is connected with the external power and is controlled by the industrial tablet computer 11, the electric power of the rotating motor 24 is controlled by the industrial tablet computer 11 to be turned on or off, the top of the curtain 26 is connected with the conveying disinfection cylinder 2, the bottom of the curtain 26 is not connected with the conveying disinfection cylinder 2, the position irradiated by the first ultraviolet lamp 21 is covered by the curtain 26, and the rotating motor 24 is a servo motor.

The working principle of the application is as follows: the method comprises the steps that firstly, a user wipes and disinfects the inner sides of a cell separation bin 13 and a detachable operation folded plate 3 by using alcohol, the detachable operation folded plate 3 is nested outside the cell separation bin 13, so that a connecting convex plate 31 covers the top of a supporting connecting plate 14, then, a rubber clamping plate 15 is clamped outside the connecting convex plate 31 and the supporting connecting plate 14, the detachable operation folded plate 3 is fixed outside the cell separation bin 13, the disinfected glove ends of a contact-preventing sleeve 32 are sent into the cell separation bin 13 through a limit flanging barrel 33, the other ends of the contact-preventing sleeve 32 are nested at the flanging of the limit flanging barrel 33 through elastic belts 35, the contact-preventing sleeve 32 is fixed, then, a first ultraviolet lamp 21 power supply is connected through an industrial tablet computer 11, meanwhile, four groups of second ultraviolet lamp 83 power supplies are connected through the industrial tablet computer 11, the inner part of a conveying disinfection barrel 2 is disinfected through a first ultraviolet lamp 83, and the inner bin 81 and a pumping-injecting tube head 61 are disinfected through the second ultraviolet lamp 83;

then open the guard door 25, use the transparent centrifuge tube 71 to hold right amount of adipose tissue, use the blanking cap 75 to plug up the top opening of the transparent centrifuge tube 71, then place the transparent centrifuge tube 71 inside square spacing hole 721 at the top of T-shaped supporting table 72 through square base 77, spacing square base 77 and transparent centrifuge tube 71 through square spacing hole 721, then push down and press down centrifugal tube 71 and T-shaped supporting table 72 again, prevent T-shaped supporting table 72 to rotate along with the output shaft of rotating motor 24, then stir the rotating plate 731 with another hand, drive the whole screw thread swivel sleeve 73 to rotate through rotating plate 731, drive the swivel nut 73 through the screw thread swivel sleeve 74 at the same time, because the bottom of the bottom rod 781 is hinged with the top outside of swivel nut 74, one side of the clamping rod 782 is hinged with the top plate of T-shaped supporting table 72 along the edge, thereby leading the clamping rod 782 to the spacing of the clamping rod 78, make the swivel nut 74 unable to follow the screw thread swivel nut 73, thereby push the screw thread on the outside of the screw thread swivel sleeve 73 to the inner side of the base 74, push the screw thread swivel nut 783 to move the bottom rod 783 along the spacing block 783 when the square base 783 is also pushed by the side of the square base 783, drive the top of the square supporting table 72 to move along the top of the square base 783, simultaneously, drive the top of the clamping rod 783 to move towards the top of the square base 783, and drive the top of the clamping rod 783 to move along the top of the square base 77, simultaneously, so that the top of the clamping rod 783 is limited;

then the protective door 25 is closed, the power supply of the first ultraviolet lamp 21 is closed through the industrial tablet computer 11, the first ultraviolet lamp 21 is prevented from irradiating and dying cells in adipose tissues in the transparent centrifuge tube 71, then the industrial tablet computer 11 controls the electric slide rail 22 to operate, and the sliding seat 23 is pushed to drive the cell separator 7 to collide with the curtain 26 to slide in the cell separation bin 13 after the electric slide rail 22 operates;

after the cell separator 7 enters the cell separation bin 13, a user observes the sliding seat 23 and the position of the cell separator 7 through the detachable operation folded plate 3, then the user stretches one hand into the anti-contact sleeve 32, when the sliding seat 23 and the cell separator 7 slide to the pipe through groove 84 at the bottom of the pumping and injecting transition box 8 corresponding to the pumping and injecting assembly 4 near the end of the industrial tablet computer 11, the operation of the electric sliding rail 22 is controlled by the industrial tablet computer 11, the power supply of the first ultraviolet lamp 21 is switched on by the industrial tablet computer 11, so that the first ultraviolet lamp 21 is continuously disinfected and sterilized, the power supply of the second ultraviolet lamp 83 in the pumping and injecting transition box 8 corresponding to the pumping and injecting assembly 4 near the end of the industrial tablet computer 11 is switched off by the industrial tablet computer 11, the user pulls out the plug cover 75 by a hand stretching into the anti-contact sleeve 32 to be placed near the electric slide rail 22, then presses down the pressing block 85 to press the spring 88, at the moment, the pressing block 85 slides in the limiting slide groove 87, the pumping and injecting tube head 61 is driven by the clamping frame 89 to descend to pass through the tube through groove 84 and enter the transparent centrifuge tube 71, the industrial tablet computer 11 controls the hydraulic system 12 corresponding to the pumping and injecting assembly 4 near the end of the industrial tablet computer 11, so that the hydraulic system 12 is controlled to control the hydraulic rod 41 to stretch out to push the piston handle in the corresponding injection tube 43 to inject a proper amount of digestion solution in the injection tube 43 into the transparent centrifuge tube 71, then the industrial tablet computer 11 is controlled to control the hydraulic system 12 corresponding to the pumping and injecting assembly 4 near the end of the industrial tablet computer 11, the hydraulic system 12 is controlled to control the hydraulic rod 41 to reset, then the force of the pressing block 85 is released to reset the spring 88, simultaneously drives the pumping and injecting tube head 61 to reset;

after the transparent centrifuge tube 71 receives digestive solution, fat tissues in the transparent centrifuge tube 71 are digested, dissolved, soaked and digested for a period of time, after digestion is completed, undigested fat tissues are layered and floated, then the electric slide rail 22 is controlled by the industrial tablet computer 11 to continue to operate so as to drive the sliding seat 23 and the cell separator 7 to slide to the bottom of the adjacent pumping and injecting transition box 8 of the pumping and injecting transition box 8 for injecting digestive solution, the piston handles of the corresponding injection cylinders 43 are aligned with the pipe through grooves 84 at the bottom of the adjacent pumping and injecting transition box 8, then the electric slide rail 22 is controlled by the industrial tablet computer 11 to stop operating, the second ultraviolet lamp 83 in the pumping and injecting transition box 8 is disconnected by the industrial tablet computer 11, then the pressing block 85 is pressed to drive the pumping and injecting tube head 61 to enter undigested fat tissue layering in the transparent centrifuge tube 71, the corresponding hydraulic rod 41 in the pumping and injecting transition box 8 is controlled by the industrial tablet computer 11, the corresponding hydraulic rod 41 is controlled by the corresponding hydraulic rod 41 to pull the corresponding injection cylinder 43 piston handle of the corresponding injection cylinder 43, so that the injection cylinder 43 is pumped out of the transparent fat tissues in the transparent centrifuge tube 71 through the pumping and injecting tube head 61;

after extracting undigested adipose tissue layering substances, releasing the force of pressing the pressing block 85 by a user, resetting the pumping and injecting tube head 61, embedding the plug cover 75 at the top of the transparent centrifuge tube 71 by the user through a hand extending into the anti-contact sleeve 32, switching on the power supply of the rotating motor 24 through the industrial tablet personal computer 11, and operating the rotating motor 24 to drive the T-shaped supporting table 72 and the whole cell separator 7 to rotate, wherein the transparent centrifuge tube 71 also drives the digested adipose tissues in the transparent centrifuge tube 71 to centrifuge for a period of time along with the rotation;

after the digested adipose tissue in the transparent centrifuge tube 71 is centrifuged, at this time, the adipose tissue in the transparent centrifuge tube 71 is continuously layered, at this time, the fat stem cell mass is sunk, other substances float upwards, then a user pulls out the plug cap 75 and places the plug cap on the electric slide rail 22 by a hand stretching into the anti-contact sleeve 32, meanwhile, the electric slide rail 22 is controlled by the industrial tablet computer 11 to continuously operate so as to drive the sliding seat 23 and the cell separator 7 to slide to the bottom of the adjacent suction and injection transition box 8 of the used suction and injection transition box 8, the transparent centrifuge tube 71 is aligned with the tube through groove 84 of the adjacent suction and injection transition box 8, at the same time, the substances floating on the adipose tissue centrifugal layering are extracted in a repeated manner of extracting undigested adipose tissue layering substances, the fat stem cell mass is left, then the electric slide rail 22 is controlled by the industrial tablet computer 11 to continuously operate so as to drive the sliding seat 23 and the cell separator 7 to slide to the suction and injection transition box 8 corresponding to the next injection assembly 4 with buffer solution, at this time, the transparent centrifuge tube 71 is enabled to be opened to the groove 84, and at the same time, the fat stem cell mass is repeatedly inserted into the sleeve 75 by the hand of the user to be separated by the anti-contact with the anti-contact sleeve 32;

after the adipose-derived stem cells are collected and separated, the first ultraviolet lamp 21 is disconnected through the industrial tablet personal computer 11, then the electric sliding rail 22 is controlled by the industrial tablet personal computer 11 to reset to drive the cell separator 7 to return to the bottom of the protective door 25 in the conveying disinfection cylinder 2, the protective door 25 is opened, the transparent centrifuge tube 71 and the T-shaped supporting table 72 are pressed and pressed, the T-shaped supporting table 72 is prevented from rotating along with the output shaft of the rotating motor 24, the rotating plate 731 is then reversely stirred by the other hand, the screw sleeve 74 slides towards the bottom to drive the bottom rods 781 in the four groups of clamping rods 78 to pull down the clamping rods 782, and at the moment, the four groups of clamping rods 78 are loosened to take out the transparent centrifuge tube 71.

To sum up; according to the adipose-derived stem cell collecting device, when stem cells are extracted, medical staff can be prevented from contacting with the syringe for extracting or injecting substances for a long time, bacteria and viruses can be effectively prevented from entering adipose tissues in the centrifuge tube, and when digestive fluid is injected into the adipose tissues, the digested substances are extracted and buffer fluid is injected into the adipose tissues, the adipose-derived stem cell collecting device is not required to be completed by using the syringe by one hand of the medical staff, so that the burden of the medical staff is reduced; meanwhile, when the adipose tissues are centrifuged to obtain stem cell clusters, the centrifugal tubes can be prevented from being thrown out by centrifugal force, and damage to the centrifugal tubes caused by collision can be avoided.

The previous description of the disclosed embodiments is provided to enable any person skilled in the art to make or use the present application. Various modifications to these embodiments will be readily apparent to those skilled in the art, and the generic principles defined herein may be applied to other embodiments without departing from the spirit or scope of the application. Thus, the present application is not intended to be limited to the embodiments shown herein but is to be accorded the widest scope consistent with the principles and novel features disclosed herein, and it should be understood that modifications and improvements may be made by those skilled in the art without departing from the spirit of the application. Accordingly, the scope of protection of the present application is to be determined by the appended claims.

Claims

1. The utility model provides a fat stem cell collection system, includes brake box frame (1), its characterized in that: the novel anti-slip type brake box comprises a brake box frame (1), and is characterized in that a limiting clamping plate (5) is arranged at the top of the brake box frame (1), an extraction and injection assembly (4) is slidably connected to the limiting clamping plate (5), an anti-slip type guide hose (6) is connected to the bottom of the extraction and injection assembly (4), an extraction and injection transition box (8) is connected to one end of the anti-slip type guide hose (6), a conveying disinfection cylinder (2) is arranged at one end of the brake box frame (1) and is communicated with the transmission disinfection cylinder, a detachable operation folded plate (3) is arranged at one side of the brake box frame (1), annular clamping grooves (51) are formed in one side of the limiting clamping plate (5) at equal intervals, and limiting clamping grooves (52) are formed in the inner side walls of the annular clamping grooves (51); the drawing and injecting transition box (8) comprises an inner bin (81), a turnover door (82), a second ultraviolet lamp (83), an outlet pipe through groove (84), a pressing block (85), a limiting pipe (86), a limiting sliding groove (87), a spring (88) and a clamping frame (89), wherein the outer side of the inner bin (81) is hinged with the turnover door (82), one side of the inner bin (81) is provided with the second ultraviolet lamp (83), the outlet pipe through groove (84) is formed in the bottom of the inner bin (81), the limiting sliding groove (87) is formed in the other side of the inner bin (81) and penetrates through the side plate of the other side of the inner bin (81), one side of the pressing block (85) is connected to the inner side of the limiting sliding groove (87) in a sliding manner, the spring (88) is connected to the bottom of the pressing block (85), the part of the pressing block (85) which is connected to the limiting sliding groove (87) is provided with the clamping frame (89), and the top of the inner bin (81) is provided with the limiting pipe (86); the bottom of the pressing block (85) is a pressing plate (852), one side of the top of the pressing block (85) is a sliding block (851), the inner side of the clamping frame (89) is provided with a clamping groove (891), and the inner side of the clamping groove (891) is provided with a magnetic sheet (892).

2. The adipose-derived stem cell collection device of claim 1, wherein: cell separator (7) still is connected with to transport disinfection section of thick bamboo (2) inboard, cell separator (7) are including transparent centrifuging tube (71), T shape brace table (72), screw thread swivel sleeve (73), swivel nut (74), cock cap (75), square spacing groove (76), square base (77) and clamping lever (78), T shape brace table (72) top is connected with transparent centrifuging tube (71), screw thread swivel sleeve (73) are connected in T shape brace table (72) outside through the bearing, square spacing hole (721) have been seted up at T shape brace table (72) top, screw thread swivel nut (74) are in screw thread swivel sleeve (73) outside through threaded connection, clamping lever (78) bottom articulates in screw thread sleeve (74) top limit position, cock cap (75) even imbeds in transparent (71) top, transparent centrifuging tube (71) bottom is square base (77), square spacing groove (76) have been seted up on square base (77) four sides.

3. The adipose-derived stem cell collection device of claim 2, wherein: the clamping rod (78) comprises a bottom rod (781), a clamping rod (782) and a limiting clamping block (783), the bottom of the clamping rod (782) is hinged to the top of the bottom rod (781), and the limiting clamping block (783) is arranged on one side of the top of the clamping rod (782); the top outer edge of the threaded rotary sleeve (73) is provided with a limiting edge plate (732), and the bottom outer edge of the threaded rotary sleeve (73) is provided with a rotary plate (731).

4. The adipose-derived stem cell collection device of claim 1, wherein: the injection drawing assembly (4) comprises a hydraulic rod (41), a connecting assembly (42), a syringe (43), a limiting ring plate (44), a top connector (45), a fastening limiting ring (46), connecting threads A (47) and an inclined guide pipe (48), wherein the bottom of the hydraulic rod (41) is connected to the top of the syringe (43) through the connecting assembly (42), the inclined guide pipe (48) is arranged at the bottom of the syringe (43), the limiting ring plate (44) is arranged at the outer edge position of the bottom of the syringe (43), the top connector (45) is arranged at the top of a piston handle of the syringe (43), the connecting threads A (47) are arranged at the outer side of the top connector (45), and the fastening limiting ring (46) is arranged at the bottom of the inclined guide pipe (48).

5. The adipose-derived stem cell collection device of claim 4, wherein: the utility model discloses a rubber cushion (441) is equipped with at coupling assembling (42) electricity top, top ring (421) bottom is equipped with end connector (424), connecting screw thread B (422) have been seted up in end connector (424) outside, and end connector (424) outside is passed through connecting screw thread B (422) are connected with threaded sleeve (423), spacing ring plate (44) both sides top is equipped with rubber cushion (441).

6. The adipose-derived stem cell collection device of claim 1, wherein: the anti-slip type material guiding hose (6) is sequentially provided with a pumping and injecting tube head (61), a connecting rubber block (62), a clamping head (63), an infusion speed regulator (64) and a clamping head (65), the pumping and injecting tube head (61) and the clamping head (65) are respectively arranged at two ends of the anti-slip type material guiding hose (6), an armature ring (66) is nested outside the connecting rubber block (62), an elastic leather sheath (67) is arranged outside the clamping head (63), and a second elastic leather sheath (68) is arranged outside the clamping head (65).

7. The adipose-derived stem cell collection device of claim 1, wherein: the utility model discloses a detachable operation folded plate, including detachable operation folded plate (3), including connecting protruding board (31), detachable operation folded plate (3) top both sides are equipped with connection protruding board (31), and detachable operation folded plate (3) riser middle part is equipped with spacing turn-ups section of thick bamboo (33), spacing turn-ups section of thick bamboo (33) one end is connected with anti-contact oversleeve (32), anti-contact oversleeve (32) one end is equipped with hollow layer (34), hollow layer (34) inboard is equipped with elastic cord (35).

8. The adipose-derived stem cell collection device of claim 1, wherein: the brake box frame (1) comprises an industrial tablet personal computer (11), a hydraulic system (12), a cell separation bin (13), a supporting connecting plate (14) and a rubber clamping plate (15), wherein the supporting connecting plate (14) is arranged on the outer sides of two ends of the top of the cell separation bin (13), the industrial tablet personal computer (11) is arranged at one end of the cell separation bin (13), the hydraulic system (12) is arranged on one side of the cell separation bin (13), and the rubber clamping plate (15) is nested on the outer side of the supporting connecting plate (14).

9. The adipose-derived stem cell collection device of claim 1, wherein: the utility model provides a conveying disinfection section of thick bamboo (2) include first ultraviolet lamp (21), electronic slide rail (22), sliding seat (23), rotating electrical machines (24), guard gate (25) and curtain (26), sliding seat (23) are installed at electronic slide rail (22) slide rail panel top, install in sliding seat (23) inboard rotating electrical machines (24), guard gate (25) articulate in conveying disinfection section of thick bamboo (2) one end, first ultraviolet lamp (21) are installed in conveying disinfection section of thick bamboo (2) both sides, curtain (26) are installed in conveying disinfection section of thick bamboo (2) and brake box frame (1) intercommunication department and guard gate (25) installation department lateral part are close to the inboard.