CN113680542A

CN113680542A - Adipose-derived stem cell collection device

Info

Publication number: CN113680542A
Application number: CN202110981085.6A
Authority: CN
Inventors: 黄勇
Original assignee: Individual
Current assignee: Hunan Tongnian Holdings Co ltd
Priority date: 2021-08-25
Filing date: 2021-08-25
Publication date: 2021-11-23
Anticipated expiration: 2041-08-25
Also published as: CN113680542B

Abstract

The invention discloses an adipose-derived stem cell collecting device, which relates to the technical field of stem cells and comprises a brake box frame, wherein the top of the brake box frame is provided with a limiting clamping plate, the limiting clamping plate is connected with a pumping and injecting assembly in a sliding manner, the bottom of the pumping and injecting assembly is connected with an anti-skidding type guide hose, one end of the anti-skidding type guide hose is connected with a pumping and injecting transition box, one end of the brake box frame is provided with a conveying and disinfecting cylinder, the conveying and disinfecting cylinder and the pumping and injecting transition box are communicated, one side of the brake box frame is provided with a detachable operation folded plate, one side of the limiting clamping plate is equidistantly provided with annular clamping grooves, and the side wall of the inner side of each annular clamping groove is provided with a limiting clamping groove; not only can avoid medical personnel and the syringe of extraction or injection material to contact with the matter centrifuging tube for a long time, can effectually avoid bacterium and virus to get into the inside adipose tissue of centrifuging tube, simultaneously, when obtaining the stem cell group with the adipose tissue centrifugation, can prevent that the centrifuging tube from being thrown away by centrifugal force, also can avoid the centrifuging tube to take place to collide with and cause the damage.

Description

Adipose-derived stem cell collection device

Technical Field

The invention relates to the technical field of stem cells, in particular to an adipose-derived stem cell collecting device.

Background

Adipose tissue is abundant in human body, a large number of adipose-derived stem cells obtained by liposuction have self-renewal, proliferation and multidirectional differentiation potential, can be differentiated into adipocytes, chondrocytes, myocytes, osteoblasts, nerve cells, glial cells and islet cells, can secrete various angiogenesis promoting factors and anti-apoptosis factors to resist inflammation and oxidation, can resist the damage of oxygen free radicals, and is expected to become a stem cell source for repairing damaged tissues and organs;

when adipose tissue stem cells are extracted, adipose tissues need to be digested and decomposed, then the obtained adipose tissue stem cell mass is subjected to centrifugation and buffer solution injection and is resuspended, when digestive fluid is injected into the adipose tissues, digested substances are extracted, and buffer solution is injected, a medical worker uses a syringe with one hand and grasps a centrifugal tube filled with the adipose tissues with the other hand, and then the operations are completed through the syringe, the medical worker directly contacts the syringe and the centrifugal tube filled with the adipose tissues for a long time to bring bacteria and viruses into the adipose tissues in the centrifugal tube to a certain extent, and meanwhile, the medical worker uses the syringe with one hand to extract the undigested adipose tissues in the centrifugal tube, so that the burden of the medical worker is increased;

when adipose tissue used centrifugal equipment to carry out centrifugal separation, all put centrifuge tube to one side and lay inside the centrifuge tube for the centrifuge tube is thrown away by centrifugal force easily, and the centrifuging tube is laid in the centrifuge tube back, takes place to collide with easily when the centrifugation, thereby causes the centrifuging tube to damage.

Disclosure of Invention

Therefore, in order to solve the above-mentioned disadvantages, the present invention provides an adipose-derived stem cell collecting device, which can prevent medical staff from contacting with a syringe for extracting or injecting a substance with a centrifugal tube for a long time during stem cell extraction, and can effectively prevent bacteria and viruses from entering adipose tissues inside the centrifugal tube, and can reduce the burden on the medical staff by not requiring the medical staff to use the syringe with one hand when injecting digestive juice, extracting a digested substance, and injecting a buffer solution into the adipose tissues; simultaneously, when obtaining the stem cell group with adipose tissue centrifugation, can prevent that the centrifuging tube from being thrown away by centrifugal force, also can avoid the centrifuging tube to take place to collide with and cause the damage.

The invention is realized in such a way that an adipose-derived stem cell collecting device is constructed, and the adipose-derived stem cell collecting device comprises a brake box frame, wherein the top of the brake box frame is provided with a limiting clamping plate, the limiting clamping plate is connected with a pumping and injecting assembly in a sliding manner, the bottom of the pumping and injecting assembly is connected with an anti-skidding guide hose, one end of the anti-skidding guide hose is connected with a pumping and injecting transition box, one end of the brake box frame is provided with a conveying disinfection cylinder, the conveying disinfection cylinder and the brake box frame are communicated, one side of the brake box frame is provided with a detachable operation folded plate, one side of the limiting clamping plate is provided with annular clamping grooves at equal intervals, and the side wall of the inner side of the annular clamping grooves is provided with limiting clamping grooves.

Further, the inboard cell separator that still is connected with of conveying disinfection section of thick bamboo, the cell separator includes transparent centrifuging tube, T shape brace table, the rotatory cover of screw thread, the swivel nut, the gag, square spacing groove, square base and supporting rod, T shape brace table top is connected with transparent centrifuging tube, the rotatory cover of screw thread passes through the bearing and connects in the brace table outside of T shape, square spacing hole has been seted up at T shape brace table top, the swivel nut passes through threaded connection in the rotatory cover outside of screw thread, the supporting rod bottom articulates in swivel nut top edgewise position, the gag even imbeds in transparent centrifuging tube top, transparent centrifuging tube bottom is square base, square spacing groove has been seted up to square base four sides.

Furthermore, the clamping rod comprises a bottom rod, a clamping rod and a limiting clamping block, the bottom of the clamping rod is hinged to the top of the bottom rod, and the limiting clamping block is arranged on one side of the top of the clamping rod; the outer edge of the top of the threaded rotating sleeve is provided with a limiting edge plate, and the outer edge of the bottom of the threaded rotating sleeve is provided with a rotating plate.

Further, pump notes transition box includes interior storehouse, turn over the door, second ultraviolet lamp, the exit tube leads to the groove, press the briquetting, spacing pipe, spacing spout, spring and double-layered frame, the interior storehouse outside articulates there is the door that turns over, the inside one side of interior storehouse is equipped with second ultraviolet lamp, the exit tube leads to the groove and offers in interior storehouse bottom, spacing spout offers in the inside opposite side of interior storehouse, and link up the inside opposite side curb plate in interior storehouse, press briquetting one side sliding connection in spacing spout inboard, press briquetting bottom to be connected with the spring, press briquetting sliding connection in the part of spacing spout to be equipped with double-layered frame, spacing pipe has been seted up at interior storehouse top.

Furthermore, a pressing plate is arranged at the bottom of the pressing block, a sliding block is arranged on one side of the top of the pressing block, a clamping groove is formed in the inner side of the clamping frame, and magnetic sheets are arranged on the inner side of the clamping groove.

Further, take out notes subassembly includes hydraulic stem, coupling assembling, injection tube, spacing ring board, top connector, fastening spacing ring, connecting thread A and pipe chute, and the hydraulic stem bottom is connected in the injection tube top through coupling assembling, and the injection tube bottom is located to the pipe chute, and the position is followed outward in the injection tube bottom to the spacing ring board, and injection tube piston handle top is located to the top connector, and the top connector outside is equipped with connecting thread A, and the pipe chute bottom is equipped with the fastening spacing ring.

Further, coupling assembling electricity top is equipped with the apical ring, and the apical ring bottom is equipped with end connector, and connecting thread B has been seted up in the end connector outside, and the end connector outside is connected with the thread bush through connecting thread B, and spacing crown plate both sides top is equipped with the rubber cushion.

Furthermore, anti-skidding formula guide hose is equipped with in proper order takes out notes tube head, connection rubber piece, chucking head, infusion speed regulator and dop, takes out notes tube head and dop and locates anti-skidding formula guide hose both ends respectively, and the nested armature ring that has in the connection rubber piece outside, and the chucking head outside is equipped with an elasticity leather sheath, and the dop outside is equipped with No. two elasticity leather sheaths.

Further, dismantlement formula operation folded plate top both sides are equipped with the connection flange, and dismantlement formula operation folded plate riser middle part is equipped with spacing turn-ups section of thick bamboo, and spacing turn-ups section of thick bamboo one end is connected with prevents contact oversleeve, prevents that contact oversleeve one end is equipped with the hollow layer, and the hollow layer inboard is equipped with the elastic cord.

Further, the brake box frame includes industry panel computer, hydraulic system, cell separation storehouse, support fishplate bar and rubber cardboard, supports the fishplate bar and locates the cell separation storehouse top both ends outside, and cell separation storehouse one end is located to industry panel computer, and hydraulic system locates cell separation storehouse one side, and the rubber cardboard nestification is in supporting the fishplate bar outside.

Further, a conveying disinfection section of thick bamboo includes first ultraviolet lamp, electronic slide rail, sliding seat, rotating electrical machines, guard gate and curtain, and the sliding seat is installed at the slide rail panel top of electronic slide rail, and the rotating electrical machines is installed to the sliding seat inboard, and the guard gate articulates in a conveying disinfection section of thick bamboo one end, and first ultraviolet lamp installs in a conveying disinfection section of thick bamboo both sides, and the curtain is installed in a conveying disinfection section of thick bamboo and brake box frame intercommunication department and guard gate installation department lateral part and is leaned on the inboard.

Compared with the prior art, the invention has the beneficial effects that:

the method has the advantages that: when the fat stem cell extraction device is used for injecting or extracting substances into or from the interior of the centrifugal tube, one hand of a medical worker extends into the contact-proof sleeve to enter the cell separation bin to press the pressing block for assistance, and then the other hand of the medical worker injects or extracts the substances into or from the interior of the centrifugal tube by operating the industrial tablet personal computer and matching with the pumping and injecting assembly, the hydraulic system and the anti-slip material guide hose, so that the medical worker is effectively prevented from contacting the syringe for extracting or injecting the substances with the centrifugal tube for a long time, and bacteria and viruses can be effectively prevented from entering fat tissues in the centrifugal tube.

The method has the advantages that: when medical personnel inject digestive juice, extract the material after the digestion and inject into the buffer solution for adipose tissue, all accomplish through controlling industry panel computer cooperation pump injection subassembly, hydraulic system and anti-skidding formula guide hose, do not need medical personnel one hand to use the syringe to accomplish, alleviate medical personnel's burden greatly.

The method has the advantages that: in the invention, the centrifugal tube is a transparent centrifugal tube, the transparent centrifugal tube is placed inside a square limiting hole at the top of a T-shaped support platform through a square base, the square base and the transparent centrifugal tube are limited through the square limiting hole, then the rotating plate is stirred by the other hand, the whole threaded rotating sleeve is driven to rotate through the rotating plate, the threaded sleeve is pushed to slide outside the threaded rotating sleeve, when the threaded sleeve slides along the plate direction along the limiting direction, the threaded sleeve drives a bottom rod to move towards the top, and simultaneously the bottom of a clamping rod is pushed to expand outwards through the bottom rod, so that the top of the clamping rod drives a limiting clamping block to gather towards a square limiting groove of the square base, at the moment, the tops of four groups of clamping rods drive the limiting clamping blocks to gather towards the square limiting groove of the square base until the limiting clamping blocks are embedded into the inner side of the square limiting groove, the square base is rapidly clamped, and the transparent centrifugal tube is also clamped, can prevent that transparent centrifuging tube from being thrown away by centrifugal force, also can avoid transparent centrifuging tube to take place to collide with and cause the damage.

Drawings

FIG. 1 is a schematic structural diagram of an adipose-derived stem cell collection device according to the present invention;

FIG. 2 is a front view of the present invention;

FIG. 3 is a cross-sectional view of the transfer sterilization cartridge of the present invention;

FIG. 4 is a schematic view of the structure of the rotating electric machine of the present invention;

FIG. 5 is a schematic view of a cell separation chamber according to the present invention;

FIG. 6 is an enlarged view of a portion of the support tab of FIG. 5 of the present invention;

FIG. 7 is a schematic view of the disassembled operating flap of the present invention;

FIG. 8 is a schematic view of the anti-contact sleeve structure of the present invention;

FIG. 9 is an enlarged view of a portion of the invention at FIG. 8M;

FIG. 10 is a schematic view of the construction of the pump assembly of the present invention;

FIG. 11 is a schematic view of the syringe barrel of the present invention;

FIG. 12 is an enlarged view of a portion of the connection assembly of FIG. 10 in accordance with the present invention;

FIG. 13 is a schematic view of the construction of the connecting thread B of the present invention;

FIG. 14 is an enlarged view of a portion of the top connector of FIG. 11 in accordance with the present invention;

FIG. 15 is an enlarged view of a portion of the retainer ring plate of FIG. 11 in accordance with the present invention;

FIG. 16 is a schematic view of a spacing clip plate structure according to the present invention;

FIG. 17 is an enlarged view of a portion of the annular slot of FIG. 16 in accordance with the present invention;

fig. 18 is a schematic view showing the structure of the slip-proof material guide hose according to the present invention;

FIG. 19 is an enlarged view of a portion of the connecting rubber block of FIG. 18 in accordance with the present invention;

FIG. 20 is an enlarged view of a portion of the clamping head of FIG. 18 in accordance with the present invention;

FIG. 21 is an enlarged view of a portion of the cartridge of FIG. 18 in accordance with the present invention;

FIG. 22 is a schematic view of the cell separator of the present invention;

FIG. 23 is a schematic view of a T-shaped support platform of the present invention;

FIG. 24 is a schematic view of a threaded rotating sleeve according to the present invention;

FIG. 25 is a schematic view of a clamping bar configuration according to the present invention;

FIG. 26 is an enlarged view of a portion of the inner cartridge of FIG. 3 in accordance with the present invention;

FIG. 27 is a side cross-sectional view of the internal chamber of the present invention;

FIG. 28 is a top view of the spacing slide of the present invention;

FIG. 29 is a schematic view of the pressing block of the present invention;

FIG. 30 is a schematic view of a frame structure according to the present invention.

In the figure: the device comprises a brake box frame 1, a conveying disinfection cylinder 2, a detachable operation folded plate 3, a pumping and injecting component 4, a limiting clamping plate 5, an anti-skid material guide hose 6, a cell separator 7, a pumping and injecting transition box 8, an industrial tablet personal computer 11, a hydraulic system 12, a cell separation bin 13, a supporting connection plate 14, a rubber clamping plate 15, a first ultraviolet lamp 21, an electric slide rail 22, a slide seat 23, a rotating motor 24, a protective door 25, a curtain 26, a connecting convex plate 31, a contact-proof sleeve 32, a limiting flanging cylinder 33, a hollow layer 34, an elastic band 35, a hydraulic rod 41, a connecting component 42, an injection cylinder 43, a limiting ring plate 44, a top connector 45, a fastening limiting ring 46, a connecting thread A47, an inclined conduit 48, a top ring 421, a connecting thread B422, a thread sleeve 423, a bottom connector 424, a rubber cushion 441, a ring clamping groove 51, a limiting clamping groove 52, a pumping and injecting pipe head 61, a connecting rubber block 62, a head 63, a clamping head 63, an injection pipe head, The transfusion speed regulator 64, a clamping head 65, an armature ring 66, a first elastic leather sheath 67, a second elastic leather sheath 68, a transparent centrifuge tube 71, a T-shaped support platform 72, a threaded rotating sleeve 73, a threaded sleeve 74, a plug cover 75, a square limiting groove 76, a square base 77, a clamping rod 78, a square limiting hole 721, a rotating plate 731, a limiting edge plate 732, a bottom rod 781, a clamping rod 782, a limiting clamping block 783, an inner bin 81, a flap 82, a second ultraviolet lamp 83, an outlet pipe through groove 84, a pressing block 85, a limiting pipe 86, a limiting sliding groove 87, a spring 88, a clamping frame 89, a sliding block 851, a pressing plate 852, a clamping groove 891 and a magnetic sheet 892.

Detailed Description

The present invention will be described in detail below with reference to the attached drawings, and the technical solutions in the embodiments of the present invention will be clearly and completely described. All other embodiments obtained by a person of ordinary skill in the art based on the embodiments of the present invention without any creative effort belong to the protection scope of the present invention; furthermore, the terms "first," "second," "third," "upper, lower, left, right," and the like are used for descriptive purposes only and are not to be construed as indicating or implying relative importance. Meanwhile, in the description of the present invention, unless otherwise explicitly specified or limited, the terms "connected" and "connected" should be interpreted broadly, for example, as being fixedly connected, detachably connected, or integrally connected; the connection can be mechanical connection or electrical connection; may be directly connected or indirectly connected through an intermediate. To those of ordinary skill in the art, the specific meanings of the above terms in the present invention can be specifically understood, and unless otherwise defined, all technical and scientific terms used herein have the same meanings as commonly understood by one of ordinary skill in the art to which the present invention belongs.

The technical scheme of the invention is further elaborated in detail by combining the drawings and the specific embodiments of the specification, and the adipose-derived stem cell collection device provided by the embodiment of the application comprises a brake box frame 1, wherein a limiting clamping plate 5 is arranged at the top of the brake box frame 1, the limiting clamping plate 5 is connected with a pumping and injecting assembly 4 in a sliding manner, the bottom of the pumping and injecting assembly 4 is connected with an anti-skidding guide hose 6, one end of the anti-skidding guide hose 6 is connected with a pumping and injecting transition box 8, one end of the brake box frame 1 is provided with a conveying disinfection cylinder 2, the conveying cylinder and the pumping and injecting transition box are communicated, a detachable operation folded plate 3 is arranged on one side of the brake box frame 1, annular clamping grooves 51 are equidistantly formed in one side of the limiting clamping plate 5, and limiting clamping grooves 52 are formed in the inner side walls of the annular clamping grooves 51.

Referring to fig. 3 and fig. 22-23 together, the inner side of the conveying and sterilizing cylinder 2 is further connected with a cell separator 7, the cell separator 7 comprises a transparent centrifuge tube 71, a T-shaped support platform 72, a threaded rotary sleeve 73, a threaded sleeve 74, a plug cover 75, a square limiting groove 76, a square base 77 and a clamping rod 78, the top of the T-shaped support platform 72 is connected with the transparent centrifuge tube 71, the threaded rotary sleeve 73 is connected to the outer side of the T-shaped support platform 72 through a bearing, the top of the T-shaped support platform 72 is provided with a square limiting hole 721, the threaded sleeve 74 is connected to the outer side of the threaded rotary sleeve 73 through a thread, the bottom of the clamping rod 78 is hinged to the edge of the top of the threaded sleeve 74, the plug cover 75 is embedded into the top of the transparent centrifuge tube 71, the bottom of the transparent centrifuge tube 71 is the square base 77, and the square base 77 is provided with the square limiting groove 76 on four sides;

hold appropriate amount adipose tissue through transparent centrifuging tube 71, prevent adipose tissue spill transparent centrifuging tube 71 through gag 75, transparent centrifuging tube 71 places when T shape brace table 72 top through square base 77, and is spacing to square base 77 through square spacing hole 721, and holding rod 78 and square spacing groove 76 have four groups respectively, and correspond each other.

As shown in fig. 24-25, the clamping rod 78 includes a bottom rod 781, a clamping rod 782 and a limiting clamp block 783, the bottom of the clamping rod 782 is hinged to the top of the bottom rod 781, and the limiting clamp block 783 is disposed on one side of the top of the clamping rod 782; the top outer edge of the screw thread rotary sleeve 73 is provided with a limit edge plate 732, and the bottom outer edge of the screw thread rotary sleeve 73 is provided with a rotary plate 731

The middle position of one side of the clamping rod 782 is hinged with the top plate edge of the T-shaped support table 72, the bottom of the bottom rod 781 is hinged with the outer side of the top of the threaded sleeve 74, and the limiting clamping block 783 is matched with the square limiting groove 76;

the threaded sleeve 74 is prevented from sliding out of the threaded rotary sleeve 73 when sliding outside the threaded rotary sleeve 73 through the limiting edge plate 732, and a convex angle is arranged at the narrow edge of the rotary plate 731, so that a user can conveniently stir the threaded sleeve.

Referring to fig. 3 and fig. 26-29 together, the pumping transition box 8 includes an inner chamber 81, a flap 82, a second ultraviolet lamp 83, an outlet tube through slot 84, a pressing block 85, a limiting tube 86, a limiting slot 87, a spring 88 and a clamping frame 89, the flap 82 is hinged outside the inner chamber 81, the second ultraviolet lamp 83 is arranged on one side inside the inner chamber 81, the outlet tube through slot 84 is arranged at the bottom of the inner chamber 81, the limiting slot 87 is arranged on the other side inside the inner chamber 81 and penetrates through the side plate on the other side inside the inner chamber 81, one side of the pressing block 85 is slidably connected inside the limiting slot 87, the spring 88 is connected at the bottom of the pressing block 85, the clamping frame 89 is arranged at the part of the pressing block 85 slidably connected to the limiting slot 87, and the limiting tube 86 is arranged at the top of the inner chamber 81;

four groups of pumping transition boxes 8 are provided, and the four groups of pumping transition boxes 8 have the same structural principle, the clamping frame 89 and the outlet pipe through groove 84 are on the same vertical line, the anti-skid material guiding hose 6 passes through the limiting pipe 86 through the pumping pipe head 61, so that the clamping head 63 is clamped at the inner side of the limiting pipe 86 to fix the pumping pipe head 61, meanwhile, the connecting rubber block 62 is clamped at the inner side of the clamping frame 89, a user presses the spring 88 by pressing the pressing block 85 downwards, at the moment, the pressing block 85 slides in the limiting sliding groove 87, the pumping and injecting tube head 61 is driven by the clamping frame 89 to descend to pass through the outlet tube through groove 84 and enter the transparent centrifuge tube 71 to pump or inject the tissue solution, four groups of second ultraviolet lamps 83 are provided, the four groups of second ultraviolet lamps 83 are connected in parallel, meanwhile, the four groups of second ultraviolet lamps 83 are connected with an external power supply, the on-off of the power supply is controlled by the industrial tablet computer 11, the pipette tip 61, which is fixed inside the pipette transition box 8, can be sterilized by means of a second ultraviolet lamp 83.

Referring to fig. 30, the bottom of the pressing block 85 is a pressing plate 852, one side of the top of the pressing block 85 is a sliding block 851, the inner side of the clamping frame 89 is provided with a clamping groove 891, and the inner side of the clamping groove 891 is provided with a magnetic sheet 892;

when the connecting rubber block 62 is clamped on the inner side of the clamping frame 89, the armature ring 66 is clamped on the inner side of the clamping groove 891 to be in contact with the magnetic sheet 892, the armature ring 66 is attracted by the magnetic sheet 892, and the connecting rubber block 62 is prevented from sliding off.

Referring to fig. 10-11 and 14-15 together, the pumping and injecting assembly 4 includes a hydraulic rod 41, a connecting assembly 42, an injection tube 43, a limit ring plate 44, a top connector 45, a fastening limit ring 46, a connecting thread a47 and an inclined conduit 48, wherein the bottom of the hydraulic rod 41 is connected to the top of the injection tube 43 through the connecting assembly 42, the inclined conduit 48 is arranged at the bottom of the injection tube 43, the limit ring plate 44 is arranged at the outer edge of the bottom of the injection tube 43, the top connector 45 is arranged at the top of a piston handle of the injection tube 43, the outer side of the top connector 45 is provided with a connecting thread a47, and the bottom of the inclined conduit 48 is provided with the fastening limit ring 46;

the pumping and injecting components 4 have four groups, the structures of the four groups of pumping and injecting components 4 are the same, the middle two groups of pumping and injecting components 4 in the four groups of pumping and injecting components 4 are responsible for pumping unnecessary solution or other impurities in the transparent centrifuge tube 71, in addition, in the two groups of pumping and injecting components 4 close to the outer side, the injection tube 43 in the pumping and injecting component 4 close to the end of the industrial tablet computer 11 is used for containing digestive solution, the injection tube 43 in the other group of pumping and injecting components 4 is used for containing buffer solution, the fatty liver cells obtained by centrifugation can be used for resuspension conveniently, when the two groups of pumping and injecting components 4 close to the outer side are used for containing the required solution, the anti-skid type material guiding hose 6 is required to be connected to the bottom of the inclined catheter 48 firstly, the piston handle of the injection tube 43 is pulled by the hydraulic rod 41 to draw the required solution, the hydraulic rod 41 is connected with the injection tube 12 through a hydraulic oil tube, the hydraulic system 12 is electrically connected with the industrial tablet computer 11, the hydraulic rod 43 is clamped into the annular clamping groove 51, make spacing ring board 44 imbed spacing draw-in groove 52, rubber cushion 441 receives the extrusion simultaneously, after injection tube 43 slides to ring groove 51 inner end, because rubber cushion 441 receives the extrusion for spacing ring board 44 is inseparabler with spacing draw-in groove 52 is connected, prevents injection tube 43 roll-off ring groove 51.

Referring to fig. 12-13, the top of the connecting assembly 42 is provided with a top ring 421, the bottom of the top ring 421 is provided with a bottom connector 424, the outer side of the bottom connector 424 is provided with a connecting thread B422, the outer side of the bottom connector 424 is connected with a thread sleeve 423 through the connecting thread B422, and the tops of two sides of the limiting ring plate 44 are provided with rubber cushions 441;

connecting thread B422 and connecting thread A47 looks adaptation are connected, after injection tube 43 slides to ring groove 51 inner end, end connector 424 aligns with top connector 45 this moment, make top connector 45 salient imbed end connector 424 sunk part simultaneously, end connector 424 salient imbeds top connector 45 sunk part, then screw thread cover 423, make screw thread cover 423 slide to top connector 45 through connecting thread B422, make screw thread cover 423 cover end connector 424 and top connector 45 junction, when hydraulic stem 41 pulled or moved back the piston handle of pushing injection tube 43, through end connector 424 and the mutual embedding centre gripping of top connector 45, reduce the connecting pressure of screw thread cover 423.

Referring to fig. 18-21, the anti-slip material guiding hose 6 is sequentially provided with a pumping hose head 61, a connecting rubber block 62, a clamping head 63, a transfusion speed regulator 64 and a clamping head 65, the pumping hose head 61 and the clamping head 65 are respectively arranged at two ends of the anti-slip material guiding hose 6, an iron ring 66 is nested outside the connecting rubber block 62, a first elastic leather sheath 67 is arranged outside the clamping head 63, and a second elastic leather sheath 68 is arranged outside the clamping head 65;

anti-skidding formula guide hose 6 has four groups, when chucking head 63 card is inside at spacing pipe 86, embed in the spacing pipe 86 outside through an elasticity leather sheath 67, can prevent that the part that anti-skidding formula guide hose 6 got into interior storehouse 81 from driving other parts of anti-skidding formula guide hose 6 and slide in interior storehouse 81 inside, and simultaneously, imbed dop 65 inboard in the pipe 48 bottom of sloping, with No. two elasticity leather sheaths 68 nestification in the fastening spacing ring 46 outside, make No. two elasticity leather sheaths 68 with fastening spacing ring 46 parcel chucking, can effectually prevent that anti-skidding formula guide hose 6 from the pipe 48 bottom landing of sloping, infusion speed regulator 64 is unanimous with venous transfusion velocity of flow regulator principle.

Referring to fig. 7-9, the detachable operation folded plate 3 has connecting convex plates 31 on both sides of the top, a limiting flanging cylinder 33 is disposed in the middle of the vertical plate of the detachable operation folded plate 3, one end of the limiting flanging cylinder 33 is connected with a contact-proof sleeve 32, one end of the contact-proof sleeve 32 is provided with a hollow layer 34, and the inner side of the hollow layer 34 is provided with an elastic band 35;

the detachable operation folded plate 3 is a transparent plate, the detachable operation folded plate 3 is nested at the outer side of the cell separation bin 13, the connecting convex plate 31 covers the top of the supporting connecting plate 14, then the rubber clamping plate 15 is clamped at the outer parts of the connecting convex plate 31 and the supporting connecting plate 14, the detachable operation folded plate 3 is fixed at the outer side of the cell separation bin 13, then the glove end of the anti-contact oversleeve 32 is sent into the cell separation bin 13 through the limiting flanging barrel 33, the other end of the anti-contact oversleeve 32 is nested at the flanging position of the limiting flanging barrel 33 through the elastic band 35, the anti-contact oversleeve 32 is fixed well, meanwhile, the elastic band 35 at the other end of the anti-contact oversleeve 32 is taken out from the flanging position of the limiting flanging barrel 33, and the anti-contact oversleeve 32 is convenient to replace and clean.

Referring to fig. 1-3 and 5-6 together, the brake box frame 1 includes an industrial tablet computer 11, a hydraulic system 12, a cell separation chamber 13, a supporting connection plate 14 and a rubber clamping plate 15, wherein the supporting connection plate 14 is disposed at the outer sides of the two ends of the top of the cell separation chamber 13, the industrial tablet computer 11 is disposed at one end of the cell separation chamber 13, the hydraulic system 12 is disposed at one side of the cell separation chamber 13, and the rubber clamping plate 15 is nested at the outer side of the supporting connection plate 14;

the hydraulic systems 12 are provided with four groups, and are respectively connected with the hydraulic rods 41 of the pumping and injecting assemblies 4 correspondingly through hydraulic oil pipes, and the four groups of hydraulic systems 12 are respectively electrically connected with the industrial tablet computer 11 and are independently controlled by the industrial tablet computer 11.

Referring to fig. 1-4 together, the conveying disinfection cylinder 2 includes a first ultraviolet lamp 21, an electric slide rail 22, a slide seat 23, a rotating motor 24, a protective door 25 and a curtain 26, the slide seat 23 is installed on the top of the slide rail panel of the electric slide rail 22, the rotating motor 24 is installed on the inner side of the slide seat 23, the protective door 25 is hinged to one end of the conveying disinfection cylinder 2, the first ultraviolet lamp 21 is installed on both sides of the conveying disinfection cylinder 2, the curtain 26 is installed at the communication position of the conveying disinfection cylinder 2 and the brake box frame 1 and the side part of the installation position of the protective door 25 is close to the inner side;

electronic slide rail 22 and industrial tablet computer 11 electric connection, and controlled by industrial tablet computer 11, it slides to drive the slide rail panel through the electronic slide rail 22 of industrial tablet computer 11 control, first ultraviolet lamp 21 connects external power supply, and open and close through industrial tablet computer 11 control power supply, the rotating electrical machines 24 output shaft passes sliding seat 23 inner chamber roof through the bearing, the part fixed connection that it passed is in T shape brace table 72 bottom, and simultaneously, rotating electrical machines 24 connects external power supply and is controlled by industrial tablet computer 11, the opening and closing of industrial tablet computer 11 control rotating electrical machines 24 power supply, curtain 26 top is connected with conveying disinfection section of thick bamboo 2, curtain 26 bottom is not connected with conveying disinfection section of thick bamboo 2, cover the position that shines first ultraviolet lamp 21 through curtain 26, rotating electrical machines 24 is servo motor.

The working principle of the invention is as follows: the user firstly uses alcohol to wipe and disinfect the inner sides of the cell separation bin 13 and the detachable operation folded plate 3, the detachable operation folded plate 3 is nested at the outer side of the cell separation bin 13, so that the connecting convex plate 31 covers the top of the supporting connecting plate 14, then the rubber clamping plate 15 is clamped at the outer parts of the connecting convex plate 31 and the supporting connecting plate 14, the detachable operation folded plate 3 is fixed at the outer side of the cell separation bin 13, the sterilized glove end of the anti-contact oversleeve 32 is sent into the cell separation bin 13 through the limiting flanging barrel 33, so that the other end of the anti-contact oversleeve 32 is nested at the flanging part of the limiting flanging barrel 33 through the elastic band 35, the anti-contact oversleeve 32 is fixed, then the power supply of the first ultraviolet lamp 21 is connected through the industrial tablet computer 11, meanwhile, the power supplies of four groups of second ultraviolet lamps 83 are connected through the industrial tablet computer 11, and the interior of the conveying disinfection barrel 2 is disinfected through the first ultraviolet lamp 21, the inner bin 81 and the pumping and injection pipe head 61 are disinfected by a second ultraviolet lamp 83;

then the protective door 25 is opened, a proper amount of adipose tissues are loaded by using a transparent centrifuge tube 71, the top opening of the transparent centrifuge tube 71 is plugged by using a plug cover 75, then the transparent centrifuge tube 71 is placed at the inner side of a square limiting hole 721 at the top of a T-shaped support platform 72 through a square base 77, the square base 77 and the transparent centrifuge tube 71 are limited through the square limiting hole 721, then the pressing centrifuge tube 71 and the T-shaped support platform 72 are pressed tightly to prevent the T-shaped support platform 72 from rotating along with the output shaft of the rotating motor 24, then the rotating plate 731 is shifted by the other hand, the whole threaded rotating sleeve 73 is driven to rotate through the rotating plate, the threaded rotating sleeve 74 is driven to rotate through the threaded rotating sleeve 73, because the bottom of a bottom rod 781 is hinged with the outer side of the top of the threaded sleeve 74, one side of a clamp rod 782 is hinged with the top edge of the T-shaped support platform 72 near the middle position, so that the T-shaped support platform 782 is limited by the T-shaped support platform 782, the clamping rods 78 are guided to limit the screw sleeve 74, so that the screw sleeve 74 cannot rotate along with the screw thread, the screw thread on the outer side of the screw thread rotating sleeve 73 is meshed with the screw thread on the inner side of the screw sleeve 74, the screw sleeve 74 is pushed to slide on the outer side of the screw thread rotating sleeve 73, when the screw sleeve 74 slides towards the limiting direction along the plate 732, the screw sleeve 74 drives the bottom rod 781 to move towards the top, meanwhile, the bottom rod 781 pushes the bottom of the clamping rod 782 to expand outwards, so that the top of the clamping rod 782 drives the limiting clamping block 783 to gather towards the square limiting groove 76 of the square base 77, at the moment, the tops of the four groups of clamping rods 782 in the four groups of clamping rods 78 drive the limiting clamping block 783 to gather towards the square limiting groove 76 of the square base 77 until the limiting clamping block 783 is embedded into the square limiting groove 76, the square base 77 is rapidly clamped, and the transparent centrifuge tube 71 is also clamped;

then the protective door 25 is folded, the power supply of the first ultraviolet lamp 21 is closed through the industrial tablet personal computer 11, the first ultraviolet lamp 21 is prevented from irradiating and killing cells in the adipose tissues in the transparent centrifuge tube 71, then the industrial tablet personal computer 11 controls the electric slide rail 22 to operate, and the electric slide rail 22 drives the sliding seat 23 to drive the cell separator 7 to knock the curtain 26 to slide into the cell separation bin 13 after operating;

after the cell separator 7 enters the cell separation chamber 13, the user observes the positions of the sliding seat 23 and the cell separator 7 through the detachable operation folded plate 3, and then the user inserts one hand into the contact-proof oversleeve 32, when the sliding seat 23 and the cell separator 7 slide to the outlet pipe through groove 84 at the bottom of the drawing and injecting transition box 8 corresponding to the drawing and injecting component 4 close to the end of the industrial tablet computer 11, the industrial tablet computer 11 controls the electric slide rail 22 to stop running, the industrial tablet computer 11 is switched on to supply power to the first ultraviolet lamp 21, so that the first ultraviolet lamp 21 continues disinfection and sterilization, then the industrial tablet computer 11 is switched off the power supply of the second ultraviolet lamp 83 in the drawing and injecting transition box 8 corresponding to the drawing and injecting component 4 close to the end of the industrial tablet computer 11, and the user pulls out the plug cover 75 to be close to the electric slide rail 22 through the hand inserted into the contact-proof oversleeve 32, then, the pressing block 85 is pressed down to press the spring 88, at the moment, the pressing block 85 slides in the limiting sliding groove 87, the pumping and injecting tube head 61 is driven to descend through the clamping frame 89, pass through the outlet pipe through groove 84 and enter the transparent centrifugal tube 71, the industrial tablet computer 11 controls the hydraulic system 12 corresponding to the pumping and injecting assembly 4 close to the end of the industrial tablet computer 11, so that the hydraulic system 12 is controlled to control the hydraulic rod 41 to extend out to push the piston handle in the corresponding injection tube 43 to inject a proper amount of digestive solution in the injection tube 43 into the transparent centrifugal tube 71, then the industrial tablet computer 11 controls the hydraulic system 12 corresponding to the pumping and injecting assembly 4 close to the end of the industrial tablet computer 11, the hydraulic system 12 is controlled to control the hydraulic rod 41 to reset, then the force for pressing the pressing block 85 is released, the spring 88 resets, and simultaneously the pumping and injecting tube head 61 resets;

after the transparent centrifuge tube 71 receives the digestion solution, the adipose tissues inside the transparent centrifuge tube 71 are digested by digestion solution soaking for a period of time, after digestion is completed, undigested adipose tissues float up in layers, the industrial tablet computer 11 controls the electric slide rail 22 to continue to operate to drive the sliding seat 23 and the cell separator 7 to slide to the bottom of the pumping transition box 8 adjacent to the pumping transition box 8 filled with the digestion solution and align with the outlet pipe through groove 84 at the bottom of the adjacent pumping transition box 8, then the industrial tablet computer 11 controls the electric slide rail 22 to stop operating, the industrial tablet computer 11 disconnects the power supply of the second ultraviolet lamp 83 inside the pumping transition box 8, then the pressing block 85 is pressed in the manner to drive the pumping tube head 61 to enter the undigested adipose tissue layers inside the transparent centrifuge tube 71, and the industrial tablet computer 11 controls the hydraulic rod 41 in the pumping assembly 4 corresponding to the pumping transition box 8 adjacent to the pumping transition box 8 filled with the digestion solution to be connected with the pumping transition box 8 The corresponding hydraulic system 12 controls the corresponding hydraulic rod 41 to be pulled up through the hydraulic system 12 to pull up the piston handle of the corresponding injection cylinder 43, so that the injection cylinder 43 pumps the undigested fat tissue stratified substance in the transparent centrifuge tube 71 out to the inside of the injection cylinder 43 through the pumping tube head 61;

after extracting the undigested fat tissue layered substances, the user loosens the force for pressing the pressing block 85, the suction and injection tube head 61 is reset, the plug cover 75 is embedded into the top of the transparent centrifuge tube 71, the user then embeds the plug cover 75 into the top of the transparent centrifuge tube 71 by the hand extending into the anti-contact oversleeve 32, then the power supply of the rotating motor 24 is switched on through the industrial tablet personal computer 11, the rotating motor 24 operates to drive the T-shaped support platform 72 and the whole cell separator 7 to rotate, and at the moment, the transparent centrifuge tube 71 also drives the digested fat tissue in the transparent centrifuge tube 71 to centrifuge for a period of time along with the rotation;

after the fat tissue digested inside the transparent centrifuge tube 71 is centrifuged, the fat tissue inside the transparent centrifuge tube 71 is layered continuously, the fat stem cell mass sinks, other substances float, then a user pulls out the plug cover 75 by the hand stretching into the anti-contact oversleeve 32 and places the plug cover on the electric slide rail 22, meanwhile, the electric slide rail 22 is controlled by the industrial tablet computer 11 to continuously operate to drive the sliding seat 23 and the cell separator 7 to slide to the bottom of the pumping transition box 8 adjacent to the pumping transition box 8 which is just used, so that the transparent centrifuge tube 71 is aligned with the outlet pipe through groove 84 of the adjacent pumping transition box 8, the way of extracting the undigested fat tissue layered substances is repeated to extract the substances which are floated by centrifugal layering of the fat tissue, the fat stem cell mass is left, and then the electric slide rail 22 is controlled by the industrial tablet computer 11 to continuously operate to drive the sliding seat 23 and the cell separator 7 to slide to the next pumping transition box 43 which is filled with buffer solution Pumping and injecting the transition box 8 corresponding to the injection assembly 4, aligning the transparent centrifuge tube 71 to the tube outlet through groove 84, simultaneously repeating the above-mentioned manner of injecting the digestive juice to inject the buffer solution, resuspending the adipose-derived stem cell mass, and then inserting the plug cover 75 into the top of the transparent centrifuge tube 71 by the user's hand extending into the anti-contact oversleeve 32, at this time, completing the adipose-derived stem cell collection and separation;

fat stem cell gathers the back of separating, the first ultraviolet lamp 21 power of rethread industry panel computer 11 disconnection, then rethread industry panel computer 11 control electronic slide rail 22 resets and drives cell separator 7 and return to the position of guard gate 25 bottom in the conveying disinfection section of thick bamboo 2, open guard gate 25, it presses transparent centrifuging tube 71 and T shape brace table 72 to press down, prevent that T shape brace table 72 from rotating along with rotating electrical machines 24 output shaft, then reverse stirring with another hand changes board 731, make swivel nut 74 slide toward the bottom, drive the lower lever 781 pull-down clamping lever 782 among four group's clamping levers 78, four group's clamping levers 78 loosen this moment, can take out transparent centrifuging tube 71.

In conclusion; when the adipose-derived stem cell collection device is used for extracting stem cells, medical staff can be prevented from contacting with a syringe for extracting or injecting substances by a mass centrifuge tube for a long time, bacteria and viruses can be effectively prevented from entering adipose tissues in the centrifuge tube, and when digestive juice is injected into the adipose tissues, the digested substances are extracted, and buffer solution is injected into the adipose tissues, the adipose tissue is not required to be injected by one hand of the medical staff, so that the burden of the medical staff is reduced; simultaneously, when obtaining the stem cell group with adipose tissue centrifugation, can prevent that the centrifuging tube from being thrown away by centrifugal force, also can avoid the centrifuging tube to take place to collide with and cause the damage.

The previous description of the disclosed embodiments is provided to enable any person skilled in the art to make or use the present invention. Various modifications to these embodiments will be readily apparent to those skilled in the art, and the generic principles defined herein may be applied to other embodiments without departing from the spirit or scope of the invention. Thus, the present invention is not intended to be limited to the embodiments shown herein but is to be accorded the widest scope consistent with the principles and novel features disclosed herein, and it is to be understood that various changes and modifications may be made by one of ordinary skill in the art without departing from the spirit and scope of the present application. Therefore, the protection scope of the present patent shall be subject to the appended claims.

Claims

1. The utility model provides an adipose-derived stem cell collection system, includes brake housing frame (1), its characterized in that: brake box frame (1) top is equipped with spacing grip block (5), spacing grip block (5) sliding connection has the pump injection subassembly (4), pump injection subassembly (4) bottom is connected with anti-skidding formula guide hose (6), anti-skidding formula guide hose (6) one end is connected with the pump injection transition box (8), and brake box frame (1) one end is equipped with conveying disinfection section of thick bamboo (2), and both communicate, and brake box frame (1) one side is equipped with dismantlement formula operation folded plate (3), and annular clamping groove (51) have been seted up to spacing grip block (5) one side equidistance, annular clamping groove (52) have been seted up to annular clamping groove (51) inboard lateral wall.

2. The adipose-derived stem cell collection device of claim 1, wherein: the cell separator (7) is connected to the inner side of the conveying disinfection cylinder (2), the cell separator (7) comprises a transparent centrifugal tube (71), a T-shaped support platform (72), a threaded rotating sleeve (73), a threaded sleeve (74), a plug cover (75), a square limiting groove (76), a square base (77) and a clamping rod (78), the top of the T-shaped support platform (72) is connected with the transparent centrifugal tube (71), the threaded rotating sleeve (73) is connected to the outer side of the T-shaped support platform (72) through a bearing, the top of the T-shaped support platform (72) is provided with a square limiting hole (721), the threaded sleeve (74) is connected to the outer side of the threaded rotating sleeve (73) through a thread, the bottom of the clamping rod (78) is hinged to the edge position at the top of the threaded sleeve (74), the plug cover (75) is connected to the top of the transparent centrifugal tube (71), and the bottom of the transparent centrifugal tube (71) is the square base (77), the four sides of the square base (77) are provided with square limiting grooves (76).

3. The adipose-derived stem cell collection device of claim 2, wherein: the clamping rod (78) comprises a bottom rod (781), a clamping rod (782) and a limiting clamping block (783), the bottom of the clamping rod (782) is hinged to the top of the bottom rod (781), and the limiting clamping block (783) is arranged on one side of the top of the clamping rod (782);

the outer edge of the top of the threaded rotating sleeve (73) is provided with a limiting edge plate (732), and the outer edge of the bottom of the threaded rotating sleeve (73) is provided with a rotating plate (731).

4. The adipose-derived stem cell collection device of claim 1, wherein: the pumping and injecting transition box (8) comprises an inner bin (81), a flap door (82), a second ultraviolet lamp (83), an outlet pipe through groove (84), a pressing block (85), a limiting pipe (86), a limiting sliding groove (87), a spring (88) and a clamping frame (89), the outer side of the inner bin (81) is hinged with the turnover door (82), one side of the inner bin (81) is provided with a second ultraviolet lamp (83), the outlet pipe through groove (84) is arranged at the bottom of the inner bin (81), the limit sliding groove (87) is arranged at the other side in the inner bin (81), and link up the inside opposite side curb plate in interior storehouse (81), press block (85) one side sliding connection in spacing spout (87) inboard, press block (85) bottom to be connected with spring (88), press block (85) sliding connection in spacing spout (87) the part be equipped with press from both sides frame (89), interior storehouse (81) top has been seted up spacing pipe (86).

5. The adipose-derived stem cell collection device of claim 4, wherein: the pressing block (85) is characterized in that the bottom of the pressing block (85) is a pressing plate (852), one side of the top of the pressing block (85) is a sliding block (851), a clamping groove (891) is formed in the inner side of the clamping frame (89), and a magnetic sheet (892) are formed in the inner side of the clamping groove (891).

6. The adipose-derived stem cell collection device of claim 1, wherein: draw notes subassembly (4) including hydraulic stem (41), coupling assembling (42), injection tube (43), spacing ring board (44), top connector (45), fastening spacing ring (46), connecting thread A (47) and pipe chute (48), hydraulic stem (41) bottom is passed through coupling assembling (42) connect in injection tube (43) top, injection tube (43) bottom is located in pipe chute (48), position is followed outward in injection tube (43) bottom in spacing ring board (44), injection tube (43) piston handle top is located in injection tube (45), and is equipped with connecting thread A (47) in the top connector (45) outside, and pipe chute (48) bottom is equipped with fastening spacing ring (46).

7. The adipose-derived stem cell collection device of claim 6, wherein: coupling assembling (42) electricity top is equipped with apical ring (421), apical ring (421) bottom is equipped with end connector (424), connecting thread B (422) have been seted up in end connector (424) outside, and end connector (424) outside is passed through connecting thread B (422) are connected with thread bush (423), stop collar board (44) both sides top is equipped with rubber cushion (441).

8. The adipose-derived stem cell collection device of claim 1, wherein: antiskid formula guide hose (6) are equipped with pump injection tube head (61), connection rubber piece (62), chucking head (63), infusion speed regulator (64) and dop (65) in proper order, antiskid formula guide hose (6) both ends are located respectively in pump injection tube head (61) and dop (65), it has armature ring (66) to connect rubber piece (62) outside nestification, chucking head (63) outside is equipped with an elasticity leather sheath (67), and dop (65) outside is equipped with No. two elasticity leather sheaths (68).

9. The adipose-derived stem cell collection device of claim 1, wherein: the detachable operation folded plate is characterized in that connecting convex plates (31) are arranged on two sides of the top of the detachable operation folded plate (3), a limiting flanging cylinder (33) is arranged in the middle of a vertical plate of the detachable operation folded plate (3), one end of the limiting flanging cylinder (33) is connected with a contact-preventing oversleeve (32), one end of the contact-preventing oversleeve (32) is provided with a hollow layer (34), and an elastic band (35) is arranged on the inner side of the hollow layer (34).

10. The adipose-derived stem cell collection device of claim 1, wherein: brake box frame (1) includes industry panel computer (11), hydraulic system (12), cell separation storehouse (13), support fishplate bar (14) and rubber cardboard (15), support fishplate bar (14) are located cell separation storehouse (13) top both ends outside, cell separation storehouse (13) one end is located in industry panel computer (11), cell separation storehouse (13) one side is located in hydraulic system (12), rubber cardboard (15) nestification is in the support fishplate bar (14) outside.

11. The adipose-derived stem cell collection device of claim 1, wherein: conveying disinfection section of thick bamboo (2) include first ultraviolet lamp (21), electronic slide rail (22), sliding seat (23), rotating electrical machines (24), guard gate (25) and curtain (26), sliding seat (23) are installed at the slide rail panel top of electronic slide rail (22), install sliding seat (23) inboard rotating electrical machines (24), guard gate (25) articulate in conveying disinfection section of thick bamboo (2) one end, first ultraviolet lamp (21) are installed in conveying disinfection section of thick bamboo (2) both sides, curtain (26) are installed in conveying disinfection section of thick bamboo (2) and braking boxed (1) intercommunication department and guard gate (25) installation department lateral part and are leaned on the inboard.