CN113670799A - Single-cell two-photon Raman recognition and accurate sorting instrument and sorting method thereof - Google Patents
Single-cell two-photon Raman recognition and accurate sorting instrument and sorting method thereof Download PDFInfo
- Publication number
- CN113670799A CN113670799A CN202110946681.0A CN202110946681A CN113670799A CN 113670799 A CN113670799 A CN 113670799A CN 202110946681 A CN202110946681 A CN 202110946681A CN 113670799 A CN113670799 A CN 113670799A
- Authority
- CN
- China
- Prior art keywords
- cell
- laser
- sample
- sorting
- unit
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 238000001069 Raman spectroscopy Methods 0.000 title claims abstract description 48
- 238000000034 method Methods 0.000 title claims abstract description 23
- 238000001237 Raman spectrum Methods 0.000 claims abstract description 38
- 230000000694 effects Effects 0.000 claims abstract description 21
- 230000005284 excitation Effects 0.000 claims abstract description 21
- 238000012546 transfer Methods 0.000 claims description 9
- 230000003287 optical effect Effects 0.000 claims description 5
- 230000008878 coupling Effects 0.000 claims description 3
- 238000010168 coupling process Methods 0.000 claims description 3
- 238000005859 coupling reaction Methods 0.000 claims description 3
- 230000006378 damage Effects 0.000 claims description 3
- 238000006073 displacement reaction Methods 0.000 claims description 3
- 238000003384 imaging method Methods 0.000 claims description 3
- 239000000725 suspension Substances 0.000 claims description 3
- 238000011160 research Methods 0.000 abstract description 4
- 238000013461 design Methods 0.000 abstract description 3
- 238000001514 detection method Methods 0.000 abstract description 2
- 230000003685 thermal hair damage Effects 0.000 abstract description 2
- 239000002184 metal Substances 0.000 description 3
- 238000010438 heat treatment Methods 0.000 description 2
- 230000006698 induction Effects 0.000 description 2
- 230000009022 nonlinear effect Effects 0.000 description 2
- 206010028980 Neoplasm Diseases 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 244000052616 bacterial pathogen Species 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000001010 compromised effect Effects 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 239000002689 soil Substances 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
Images
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/65—Raman scattering
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N2015/1006—Investigating individual particles for cytology
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N2015/1028—Sorting particles
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Biochemistry (AREA)
- Physics & Mathematics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Analytical Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Dispersion Chemistry (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
Abstract
The invention provides a single-cell two-photon Raman recognition and accurate sorting instrument and a sorting method thereof, wherein the sorting method comprises the following steps: the device comprises a pulse laser, a laser control unit, a sample stage, a Raman spectrum unit, a cell sorting unit, a sample cell and a cell receiving unit; the Raman spectrum excitation and detection of the single cells in the complex environment and the accurate sorting of the single cells with low thermal damage are realized; exciting Raman signals by using pulse laser, and sorting single cells; the excitation range of the Raman spectrum can be effectively controlled to be in a single cell range, and the accuracy of Raman specific identification is improved; meanwhile, the heat effect generated in the accurate single cell sorting process is reduced, and the activity of the sorted cells is improved. The system realizes the specific identification and the accurate sorting of the single cells, serves the research of the single cells, and has the advantages of scientific structural design, safe and reliable work, simple and convenient operation and easy popularization.
Description
Technical Field
The invention belongs to the technical field of Raman recognition and sorting of cells, and particularly relates to a single-cell two-photon Raman recognition and accurate sorting instrument and a sorting method thereof.
Background
Cells are the basic unit of life, and there are no two cells in the world, even if there are differences between cells in the same cell population of the same organism; only when biological research is carried out from the single cell level, the essence of life can be dialyzed, and the premise of the single cell research is the specific identification and accurate sorting of the single cells.
As a specific optical identification mode, Raman spectroscopy is widely applied to cell type analysis in the fields of tumor tissue, soil microorganism, pathogenic bacterium identification and the like, and is a single-cell specific identification technology with great potential. Specifically, under laser excitation, biomolecules vibrate and scatter the excitation laser, and a raman spectrum signal is emitted.
Under the influence of optical diffraction limit, the area excited by laser is usually larger than the size space of one cell, so that other cells and substances around the target cell can be excited to generate Raman signals, and the specific recognition of the target single cell is interfered.
On the other hand, laser-induced forward transfer techniques have been used extensively for precise sorting of individual cells. Specifically, the laser launches the metal thin layer through photothermal effect, fixed point location, realizes the accurate sorting of specific position unicell. In this process, the cells are subjected to intense heat, the activity of sorting the cells is severely affected, and biological significance is greatly compromised.
Disclosure of Invention
In order to overcome the technical defects, the invention provides a single-cell two-photon Raman recognition and accurate sorting instrument and a sorting method thereof, which realize Raman spectrum excitation and detection of single cells in complex environment and accurate sorting of single cells with low thermal damage; simultaneously, a pulse laser (such as a picosecond laser, a femtosecond laser and the like which can realize two-photon laser) is used for exciting Raman signals and sorting single cells; the excitation range of the Raman spectrum can be effectively controlled to be in a single cell range, and the accuracy of Raman specific identification is improved; meanwhile, the heat effect generated in the accurate single cell sorting process is reduced, and the activity of the sorted cells is improved. Realizes the specific recognition and the accurate sorting of the single cells, and serves the single cell research.
A single-cell two-photon Raman recognition and accurate sorting instrument and a sorting method thereof, wherein:
single cell two-photon Raman recognition and accurate sorter, comprising:
the device comprises a pulse laser, a laser control unit, a sample stage, a Raman spectrum unit, a cell sorting unit, a sample cell and a cell receiving unit;
further, the pulse laser is used for outputting pulse laser;
further, the laser control unit is configured to receive the pulse laser output by the pulse laser, and control the pulse laser to couple into the raman spectroscopy unit and the cell sorting unit;
as an illustration, the laser control unit may control the frequency, power, wavelength, pulse width of the pulsed laser; the spot shape, size, and beam number of the pulsed laser can also be controlled.
As an illustration, the pulsed laser is coupled into the laser of the raman spectroscopy unit and output through the focusing structure of the raman spectroscopy unit as an excitation laser;
as an illustration, the pulsed laser is coupled into the cell sorting unit as a sorting laser for a sorting operation of target cells;
further, the sample stage is in electrical signal connection with the raman spectroscopy unit and is used for receiving the identification result output by the raman spectroscopy unit, and the sample stage adjusts the position of the focus of the excitation laser output by the raman spectroscopy unit in the sample pool through self displacement; moving the sample stage again according to the recognition result, and adjusting the sorting laser output by the cell sorting unit to focus on the position of the sample pool corresponding to the target cell;
as an illustration, the sample stage has one or more dimensions of movement; the moving precision interval of the sample stage is as follows: between 1 nanometer and 1 micrometer;
as a preferred example, the moving precision interval of the sample stage is: between 1 nanometer and 100 nanometers;
further, the raman spectrum unit is used for outputting excitation laser to excite a raman signal of the sample, and then collecting the raman spectrum signal of the sample; judging whether the sample is a target cell or not by analyzing a Raman spectrum signal of the sample; when the Raman spectrum unit analyzes the target cells, the identification result can be obtained, and the identification result is output to the sample stage; the recognition result comprises: size, shape, and location of the target cell;
as an illustration, the raman spectroscopy unit includes: an optical microscopic imaging module;
further, the cell sorting unit is positioned at the suspension above the sample table, and can sort target cells based on a laser-induced forward transfer sorting method;
further, the sample cell is arranged below the sample table and used for loading a sample to be sorted;
further, the cell receiving unit is arranged below the sample pool and used for receiving the target cells;
the sorting method of the single-cell two-photon Raman recognition and accurate sorter comprises the following steps:
loading a sample into a sample cell, and fixing the sample cell on a sample table;
step two, starting a pulse laser and outputting pulse laser;
thirdly, the laser control unit controls the pulse laser to be coupled into two paths of laser, wherein one path of laser is input into the Raman spectrum unit to be used as excitation laser, and the other path of laser is input into the cell sorting unit to be used as sorting laser;
fourthly, controlling the sample stage to move so that the focus of the excitation laser falls on the position of the sample in the sample cell;
exciting, collecting and identifying the Raman spectrum signal of the sample in the sample pool by the Raman spectrum unit with the resolution exceeding the diffraction limit, analyzing the sample to obtain the identification result of the target cell, and outputting the identification result to the sample platform;
as an illustration, the recognition result further includes: the size, shape, location, and type of target cell;
sixthly, the sample stage moves again according to the recognition result, the sorting laser output by the cell sorting unit is adjusted to focus on the position of the sample pool corresponding to the target cell, and the target cell is sorted based on a laser-induced forward transfer sorting method;
as an example, compared with other types of sorting lasers, the sorting laser generated after coupling of the pulse laser has a lower thermal effect, so that damage to target cells caused by the thermal effect is reduced while a stable sorting effect is ensured;
seventhly, the cell receiving unit is matched with the cell sorting unit and the sample pool to receive the target cells;
the invention has the beneficial effects that:
the invention is based on the nonlinear effect, the pulse laser can excite the Raman signal with the resolution exceeding the diffraction limit, the action range can be controlled in the size range of single cells, and the invention can be completely used for exciting the Raman signal of the single cells in a cell group.
The pulse width of the pulse laser is extremely short, the duration time is far shorter than the time of the thermal effect of the metal thin layer, and the thermal effect can be inhibited. The pulsed laser is used as a laser source for laser induction forward transfer sorting of single cells, so that heating of the cells can be obviously reduced, and the activity of the sorted single cells is improved.
The invention has scientific structural design, safe and reliable work, simple and convenient operation and easy popularization.
Drawings
FIG. 1 is a schematic diagram of the overall structure of the single-cell two-photon Raman recognition and precision sorter of the present invention
Detailed Description
Preferred embodiments of the present invention will be described in detail below with reference to the accompanying drawings.
Detailed description referring to fig. 1, a single-cell two-photon raman identification and precision sorter and a sorting method thereof, wherein:
single cell two-photon Raman recognition and accurate sorter, comprising: a pulse laser 101, a laser control unit 102, a sample stage 103, a raman spectroscopy unit 104, a cell sorting unit 105, a sample cell 106, and a cell receiving unit 107;
further, the pulse laser 101 is configured to output pulse laser light;
further, the laser control unit 102 is configured to receive the pulsed laser output by the pulsed laser 101, and control the pulsed laser to be coupled into the raman spectroscopy unit 104 and the cell sorting unit 105;
as an illustration, the laser control unit 102 may control the frequency, power, wavelength, pulse width of the pulsed laser; the spot shape, size, and beam number of the pulsed laser can also be controlled.
As an illustration, the pulsed laser is coupled into the laser of the raman spectrum unit 104 and output through the focusing structure of the raman spectrum unit 104 as an excitation laser;
as an illustration, the pulsed laser is coupled into the cell sorting unit 105 as a sorting laser for a sorting operation of the target cells 108;
further, the sample stage 103 is electrically connected to the raman spectroscopy unit 104, and is configured to receive the identification result output by the raman spectroscopy unit 104, and the sample stage 103 adjusts, through self-displacement, a position where a focal point of the excitation laser output by the raman spectroscopy unit 104 falls in the sample cell 106; moving the sample stage 103 again according to the recognition result, and adjusting the sorting laser output by the cell sorting unit 105 to focus on the position of the sample cell 106 corresponding to the target cell 108;
as an illustration, the sample stage 103 has one or more dimensions of movement; the moving precision interval of the sample stage 103 is as follows: between 1 nanometer and 1 micrometer;
as an illustration, the one dimension is a movement on a rectilinear coordinate system; the plurality of dimensions includes two dimensions and three dimensions; the two dimensions are movement on a plane coordinate system; the three dimensions are movement on a three-dimensional coordinate system;
as a preferred example, the moving precision interval of the sample stage 103 is: between 1 nanometer and 100 nanometers;
further, the raman spectrum unit 104 is configured to output excitation laser to excite a raman signal of the sample, and then collect the raman spectrum signal of the sample; judging whether the sample is a target cell 108 by analyzing a Raman spectrum signal of the sample; when the raman spectrum unit 104 analyzes the target cell 108, it may obtain an identification result and output the identification result to the sample stage 103; the recognition result comprises: size, shape, and location of the target cell;
as an illustration, the raman spectroscopy unit 104 includes: an optical microscopic imaging module;
further, the cell sorting unit 105 is located in the suspension above the sample stage 103, and can sort the target cells 108 based on a laser-induced forward transfer sorting method;
further, the sample cell 106 is arranged below the sample stage 103 and used for loading a sample to be sorted;
further, the cell receiving unit 107 is disposed below the sample cell 106, and is configured to receive the target cell 108;
the sorting method of the single-cell two-photon Raman recognition and accurate sorter comprises the following steps:
loading a sample into a sample cell 106, and fixing the sample cell 106 on a sample table 103;
step two, starting the pulse laser 101 and outputting pulse laser;
thirdly, the laser control unit 102 controls the pulse laser to be coupled into two paths of laser, wherein one path of laser is input into the Raman spectrum unit 104 and is used as the excitation laser of the Raman spectrum unit 104, and the other path of laser is input into the cell sorting unit 105 and is used as the sorting laser;
fourthly, controlling the sample stage 103 to move so that the focal point of the excitation laser falls on the position of the sample in the sample cell 106;
step five, the raman spectrum unit 104 excites, collects and identifies the raman spectrum signal of the sample in the sample cell 106 with the resolution exceeding the diffraction limit, analyzes the sample to obtain the identification result of the target cell 108, and outputs the identification result 108 to the sample stage;
as an illustration, the recognition result further includes: the size, shape, location, and type of target cell;
sixthly, the sample stage 103 moves again according to the recognition result, the position of the sorting laser output by the cell sorting unit 105 is adjusted to be focused on the sample pool 106 corresponding to the target cell 108, and the target cell 108 is sorted based on a laser-induced forward transfer sorting method;
as an example, compared with other types of sorting lasers, the sorting laser generated after coupling of the pulse laser has a lower thermal effect, so that damage to the target cells 108 caused by the thermal effect is reduced while a stable sorting effect is ensured;
seventhly, the cell receiving unit 107 is matched with the cell sorting unit 105 and the sample cell 106 to receive the target cells 108;
as an illustration, the pulsed laser is used to output pulsed laser including: picosecond, femtosecond and nanosecond lasers;
the invention is based on the nonlinear effect, the pulse laser can excite the Raman signal with the resolution exceeding the diffraction limit, the action range can be controlled in the size range of single cells, and the invention can be completely used for exciting the Raman signal of the single cells in a cell group. The pulse width of the pulse laser is extremely short, the duration time is far shorter than the time of the thermal effect of the metal thin layer, and the thermal effect can be inhibited. The pulsed laser is used as a laser source for laser induction forward transfer sorting of single cells, so that heating of the cells can be obviously reduced, and the activity of the sorted single cells is improved. The invention has scientific structural design, safe and reliable work, simple and convenient operation and easy popularization.
The above embodiments are only preferred embodiments of the present invention, and it should be understood that the above embodiments are only for assisting understanding of the method and the core idea of the present invention, and are not intended to limit the scope of the present invention, and any modifications, equivalents and the like made within the spirit and principle of the present invention should be included in the scope of the present invention.
Claims (8)
1. Unicellular two-photon Raman discernment and accurate sorter, its characterized in that includes: the device comprises a pulse laser, a laser control unit, a sample stage, a Raman spectrum unit, a cell sorting unit, a sample cell and a cell receiving unit;
the pulse laser is used for outputting pulse laser;
the laser control unit is used for receiving the pulse laser output by the pulse laser and controlling the pulse laser to be coupled into the Raman spectrum unit and the cell sorting unit;
the pulse laser is coupled into the laser of the Raman spectrum unit and is output through the focusing structure of the Raman spectrum unit to serve as excitation laser; the pulse laser is coupled into the cell sorting unit and is used as sorting laser for sorting operation of target cells;
the sample stage is in electrical signal connection with the Raman spectrum unit and is used for receiving the identification result output by the Raman spectrum unit, and the position of the focus of the excitation laser output by the Raman spectrum unit in the sample pool is adjusted through self displacement of the sample stage; moving the sample stage again according to the recognition result, and adjusting the sorting laser output by the cell sorting unit to focus on the position of the sample pool corresponding to the target cell;
the Raman spectrum unit is used for outputting excitation laser to excite a Raman signal of the sample and then collecting the Raman spectrum signal of the sample; judging whether the sample is a target cell or not by analyzing a Raman spectrum signal of the sample; when the Raman spectrum unit analyzes the target cells, the identification result can be obtained, and the identification result is output to the sample stage;
the cell sorting unit is positioned at the suspension above the sample table and can sort target cells based on a laser-induced forward transfer sorting method;
the sample cell is arranged below the sample table and used for loading a sample to be sorted;
the cell receiving unit is arranged below the sample pool and used for receiving target cells.
2. The single-cell two-photon raman identification and precision sorter of claim 1, wherein the laser control unit can control the frequency, power, wavelength, pulse width of the pulsed laser; the spot shape, size, and beam number of the pulsed laser can also be controlled.
3. The single-cell two-photon raman identification and precision sorter of claim 1, wherein the sample stage has one or more moving dimensions; the moving precision interval of the sample stage is as follows: between 1 nanometer and 1 micrometer.
4. The single-cell two-photon raman identification and precision sorter of claim 1, wherein the moving precision interval of the sample stage is: between 1 nm and 100 nm.
5. The single-cell two-photon raman identification and precision sorter of claim 1, wherein the raman spectroscopy unit comprises: an optical microscopic imaging module.
6. The single-cell two-photon raman identification and precision sorter of claim 1, wherein the identification results comprise: size, shape, and location of the target cell.
7. The sorting method of the single-cell two-photon Raman recognition and accurate sorter is characterized by comprising the following steps of:
loading a sample into a sample cell, and fixing the sample cell on a sample table;
step two, starting a pulse laser and outputting pulse laser;
thirdly, the laser control unit controls the pulse laser to be coupled into two paths of laser, wherein one path of laser is input into the Raman spectrum unit to be used as excitation laser, and the other path of laser is input into the cell sorting unit to be used as sorting laser;
fourthly, controlling the sample stage to move so that the focus of the excitation laser falls on the position of the sample in the sample cell;
exciting, collecting and identifying the Raman spectrum signal of the sample in the sample pool by the Raman spectrum unit with the resolution exceeding the diffraction limit, analyzing the sample to obtain the identification result of the target cell, and outputting the identification result to the sample platform;
sixthly, the sample stage moves again according to the recognition result, the sorting laser output by the cell sorting unit is adjusted to focus on the position of the sample pool corresponding to the target cell, and the target cell is sorted based on a laser-induced forward transfer sorting method;
and seventhly, the cell receiving unit is matched with the cell sorting unit and the sample pool to receive the target cells.
8. The sorting method of the single-cell two-photon Raman recognition and precision sorter of claim 7, wherein the sorting laser generated after coupling the pulsed laser has a lower thermal effect than other types of sorting lasers, thereby ensuring a stable sorting effect and reducing damage to target cells due to the thermal effect.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110946681.0A CN113670799B (en) | 2021-08-18 | 2021-08-18 | Single-cell two-photon Raman recognition and accurate sorting instrument and sorting method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110946681.0A CN113670799B (en) | 2021-08-18 | 2021-08-18 | Single-cell two-photon Raman recognition and accurate sorting instrument and sorting method thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN113670799A true CN113670799A (en) | 2021-11-19 |
| CN113670799B CN113670799B (en) | 2023-12-26 |
Family
ID=78543486
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202110946681.0A Active CN113670799B (en) | 2021-08-18 | 2021-08-18 | Single-cell two-photon Raman recognition and accurate sorting instrument and sorting method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN113670799B (en) |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101216414A (en) * | 2007-12-29 | 2008-07-09 | 中国科学院西安光学精密机械研究所 | Multifunctional optical micro-control device |
| CN102019277A (en) * | 2010-10-29 | 2011-04-20 | 北京惟馨雨生物科技有限公司 | Sorter and sorting method for separating cells and particles |
| CN105758838A (en) * | 2016-04-11 | 2016-07-13 | 北京大学 | Method and device for detecting and selecting laser-induce enhanced Raman spectrum in liquid |
| CN205719979U (en) * | 2016-04-11 | 2016-11-23 | 北京大学 | In a kind of liquid, induced with laser strengthens detection and the sorting unit of Raman spectrum |
| WO2020007326A1 (en) * | 2018-07-06 | 2020-01-09 | 中国科学院青岛生物能源与过程研究所 | High-throughput parallel raman spectrometer based on single cell detection |
-
2021
- 2021-08-18 CN CN202110946681.0A patent/CN113670799B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101216414A (en) * | 2007-12-29 | 2008-07-09 | 中国科学院西安光学精密机械研究所 | Multifunctional optical micro-control device |
| CN102019277A (en) * | 2010-10-29 | 2011-04-20 | 北京惟馨雨生物科技有限公司 | Sorter and sorting method for separating cells and particles |
| CN105758838A (en) * | 2016-04-11 | 2016-07-13 | 北京大学 | Method and device for detecting and selecting laser-induce enhanced Raman spectrum in liquid |
| CN205719979U (en) * | 2016-04-11 | 2016-11-23 | 北京大学 | In a kind of liquid, induced with laser strengthens detection and the sorting unit of Raman spectrum |
| WO2020007326A1 (en) * | 2018-07-06 | 2020-01-09 | 中国科学院青岛生物能源与过程研究所 | High-throughput parallel raman spectrometer based on single cell detection |
Non-Patent Citations (1)
| Title |
|---|
| 蔡田雨;陈达;李抄;陈盟;杜耀华;: "表面增强拉曼光谱在细菌分类鉴定领域的研究进展", 军事医学, no. 07 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN113670799B (en) | 2023-12-26 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| DK1625203T3 (en) | EFFECTIVE SEPARATION OF haploid cells FOR FLOWCYTOMETRISYSTEMER | |
| KR101507041B1 (en) | Light irradiation method, light irradiation device, and fine particle analyzing apparatus | |
| CN102436063B (en) | Laser optical tweezer microscope | |
| JP2010019853A (en) | Optically commensurate device for capillary electrophoresis device | |
| CN107192705B (en) | The laser induced breakdown spectroscopy that multiple spot bifocal excites simultaneously enhances measuring system | |
| CN112485235B (en) | Transmission electron microscope sample rod system with ultrafast time resolution spectral capability and application | |
| CN116670492A (en) | Method and microscope for recording trajectories of individual particles in a sample | |
| CN2758757Y (en) | FS laser clamping device for trapping biological cells | |
| CN109612388B (en) | Optical measurement system and method for cell perforation | |
| CN113670799B (en) | Single-cell two-photon Raman recognition and accurate sorting instrument and sorting method thereof | |
| WO2022155033A1 (en) | Flow cytometers including light collection enhancers, and methods of using the same | |
| CN210347460U (en) | Laser-induced fluorescence detection device and laser-induced fluorescence detection system | |
| CN112285090A (en) | Portable confocal unicellular Raman scattering detecting system | |
| Uchida et al. | Micromanipulation: Whole-cell manipulation by optical trapping | |
| CN108873294A (en) | A kind of optical tweezer trapped particle of double excitation or the device of cell | |
| CN111398139A (en) | Rapid detection system for single cell spectrum averaging | |
| US20210325292A1 (en) | Systems for light detection array multiplexing and methods for same | |
| Burger et al. | Acousto‐optic laser‐scanning cytometer | |
| CN110274708B (en) | Tumor cell nanoscale quantum three-dimensional thermal imaging system | |
| CN113941377A (en) | All-glass microfluidic chip and processing method | |
| CN108831581A (en) | There are three the optical tweezer trapped particle of laser or the devices of cell for a kind of tool | |
| CN108873293A (en) | There are four the optical tweezer trapped particle of laser or the devices of cell for a kind of tool | |
| CN108931847A (en) | A kind of device of optical tweezer trapped particle or cell with seven laser | |
| CN105136745A (en) | Rapid microorganism detecting device based on mie scattering | |
| CN113502207B (en) | Multifunctional cell sorting device based on laser system and operation method |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |