CN117805062A

CN117805062A - Multi-mode spectroscopy control method for aerosol particles

Info

Publication number: CN117805062A
Application number: CN202311784333.3A
Authority: CN
Inventors: 杨文慧; 陈和; 周冬生; 陈思颖; 胡凌飞; 郭磐; 吕蒙; 杨艺敏; 罗宇鹏
Original assignee: Academy of Military Medical Sciences AMMS of PLA; Beijing Institute of Technology BIT
Current assignee: Academy of Military Medical Sciences AMMS of PLA; Beijing Institute of Technology BIT
Priority date: 2023-12-23
Filing date: 2023-12-23
Publication date: 2024-04-02

Abstract

The invention provides a multi-mode spectroscopy control method of aerosol particles, which comprises the following steps: obtaining a result of whether aerosol to be detected exists in the detection range, and if the aerosol to be detected exists in the detection range, continuing the next step; responding to a first operation instruction acted on the pulse generator, controlling the pulse generator to send a transmitting signal to the ultraviolet laser, so that the ultraviolet laser starts to transmit ultraviolet laser, and the ultraviolet laser irradiates aerosol microbeam to be detected; specifically, it includes: responding to an odd-number operation instruction acted on the ultraviolet laser, controlling the first ultraviolet laser to emit first ultraviolet laser, and controlling the multichannel acquisition system to acquire first fluorescence spectrum data corresponding to the first ultraviolet laser; responding to an even number of operation instructions acted on the ultraviolet laser, controlling the second ultraviolet laser to emit second ultraviolet laser, and controlling the multichannel acquisition system to acquire corresponding second fluorescence spectrum data; the first fluorescence spectrum data and/or the second fluorescence spectrum data are displayed or saved.

Description

Multi-mode spectroscopy control method for aerosol particles

Technical Field

The present invention relates to the field of aerosol detection, and more particularly, to a method, system, and apparatus for multi-modal spectroscopy control of aerosol particles.

Background

Currently, methods for analyzing aerosol components focus mainly on biochemical properties. The method mainly comprises a physical and chemical detection method (a conductivity detection method, a chemical analysis method and the like), a biological analysis (an ionization mass spectrometry method, a western blotting method and the like), and a method of combining optics comprises a spectrum method, a photometric analysis method, a photothermal analysis method, a fluorescence microscopy method and the like of different wave bands. Most of the methods need to collect and culture aerosol particles through steps such as filter membrane sampling and the like, then measure the aerosol particles, and meanwhile, the equipment is complex, long in time consumption or high in cost, so that the requirements of real-time detection are difficult to meet.

Based on the defects, the inventor groups research and develop a system for identifying bioaerosol particles by utilizing a laser-induced fluorescence technology in 2021, and CN 114965182A-dual-channel bioaerosol particle identification system and identification method; the system can rapidly judge whether the aerosol exists or not through the elastic scattering signal, and can judge whether the aerosol is biological aerosol or not based on reflecting organic matter components in the aerosol through fluorescence spectrum; with the continuous and deep research, the inventor team combines LIF and LIBS on the basis of the original research, and derives a more accurate, faster and simplified bioaerosol detection system.

However, the system comprising a plurality of optical-mechanical components is complex in optical path design, and the components need to be coordinated and consistent to ensure the smooth test; and the LIF and LIBS are difficult to combine in technology at present, including the increase of the LIBS to the chamber light path during combination, the time sequence requirement and the complexity among multiple paths, and the like, so that a reliable and efficient method or system is needed to intensively control and manage each component.

Disclosure of Invention

The present invention aims to solve at least one of the technical problems existing in the prior art. For this reason, the invention provides a multimode spectroscopy control method and system of aerosol particles; according to the method, through transmitting each instruction to each component, the coordination consistency among the complicated components and the time sequence requirement of the multipath light paths are ensured, the centralized control and management of the complicated system are realized, whether the aerosol exists in the detection range or not, whether the aerosol is the biological aerosol or not is judged rapidly, the types of internal elements of the biological aerosol are identified with high precision, and the accuracy of detecting the biological aerosol is greatly improved.

A first aspect of the present application discloses a method of multi-modal spectroscopy control of aerosol particles, the method comprising:

S1: obtaining a result of whether aerosol to be detected exists in the detection range, and if the aerosol to be detected exists in the detection range, continuing the next step;

s2: responding to a first operation instruction acted on the pulse generator, controlling the pulse generator to send a transmitting signal to the ultraviolet laser, and enabling the ultraviolet laser to start transmitting ultraviolet laser, wherein the ultraviolet laser irradiates aerosol microbeam to be detected; specifically, it includes: responding to an odd-number operation instruction acted on the ultraviolet laser, controlling the first ultraviolet laser to emit first ultraviolet laser, and controlling the multichannel acquisition system to acquire first fluorescence spectrum data corresponding to the first ultraviolet laser; responding to an even number of operation instructions acted on the ultraviolet laser, controlling the second ultraviolet laser to emit second ultraviolet laser, and controlling the multichannel acquisition system to acquire corresponding second fluorescence spectrum data;

s3: displaying or storing the first fluorescence spectrum data and/or the second fluorescence spectrum data.

Further, the method for judging the odd-numbered operation instruction and the even-numbered operation instruction comprises the following steps: when the remainder of dividing the current acquisition times by 2 is 0, the current acquisition times are the even number times; when the remainder of dividing the current collection times by 2 is 1, the current collection times are the even number times.

Further, after S2, the method further includes: splicing the first fluorescence spectrum data and the second fluorescence spectrum data to obtain spliced fluorescence spectrum data; displaying or storing the spliced fluorescence spectrum data;

optionally, the method further comprises: obtaining a result of whether the aerosol to be detected is biological aerosol or not based on the spliced fluorescence spectrum data;

optionally, after the first fluorescence spectrum data is collected, automatically storing the first fluorescence spectrum data;

optionally, after the second fluorescence spectrum data is collected, automatically storing the second fluorescence spectrum data;

optionally, the first ultraviolet laser is a 360nm pulse laser;

optionally, the second ultraviolet laser is a 266nm pulse laser.

Further, after S3, the method further includes: responding to a second operation instruction acted on the infrared pulse laser, controlling the infrared pulse laser to emit third infrared laser, irradiating the biological aerosol microbeam to be detected by the third infrared laser, and controlling a breakdown spectrometer to acquire breakdown spectrum data corresponding to the third infrared laser;

obtaining element types of the bioaerosol microbeam to be detected based on the breakdown spectrum data;

Optionally, after the breakdown spectroscopy data is collected, the breakdown spectroscopy automatically stores the breakdown spectroscopy data;

optionally, the infrared pulse laser is a 1064nm pulse laser.

Further, performing data processing based on the first fluorescence spectrum data, the second fluorescence spectrum data and the breakdown spectrum data to obtain a multi-mode spectrogram; or, performing data processing based on the spliced fluorescence spectrum data and breakdown spectrum data to obtain a multi-mode spectrogram;

optionally, the result of obtaining whether the aerosol to be detected exists in the detection range in S1 is obtained by the following manner: responding to a third operation instruction acted on the infrared laser, sending a signal for generating infrared laser by the infrared laser, and irradiating the aerosol micro-beam to be detected by the infrared laser;

comparing the signal of the infrared laser after irradiating the aerosol micro beam to be detected with a trigger threshold;

if the signal of the infrared laser after irradiating the aerosol micro beam to be detected is larger than or equal to the trigger threshold value, obtaining the result of the aerosol existing in the detection range, and controlling the pulse generator to send a closing signal to the infrared laser, so that the infrared laser stops generating the infrared laser;

optionally, the infrared laser includes a first infrared laser and a second infrared laser; the first infrared laser is a 780nm continuous laser and the second infrared laser is a 850nm continuous laser.

Further, the method for calculating the trigger time of the odd-numbered operation instructions includes:

acquiring the speed of an aerosol particle stream to be detected;

acquiring a first height difference of infrared laser and first ultraviolet laser;

calculating and obtaining the triggering time of a first ultraviolet laser based on the speed and the first height difference, wherein the triggering time of the first ultraviolet laser is the triggering time of the odd-number operation instruction;

optionally, the method for calculating the trigger time of the even number of operation instructions includes:

acquiring the speed of an aerosol particle stream to be detected;

acquiring a second height difference of the first ultraviolet laser and the second ultraviolet laser;

and calculating and obtaining the trigger time of the second ultraviolet laser based on the speed and the second height difference, wherein the trigger time of the second ultraviolet laser is the trigger time of the even-number operation instruction.

Further, the operation instruction includes any one or several of the following; mouse click, keyboard input, voice command.

A second aspect of the present application discloses an aerosol particle multi-modal spectroscopy control system, the system comprising:

the judging unit is used for obtaining the result of whether the aerosol to be detected exists in the detection range, and if the aerosol to be detected exists in the detection range, continuing the next step;

The interaction unit is used for responding to a first operation instruction acted on the pulse generator, controlling the pulse generator to send a transmitting signal to the ultraviolet laser, enabling the ultraviolet laser to start transmitting ultraviolet laser, and enabling the ultraviolet laser to irradiate aerosol microbeam to be detected; specifically, it includes: responding to an odd-number operation instruction acted on the ultraviolet laser, controlling the first ultraviolet laser to emit first ultraviolet laser, and controlling the multichannel acquisition system to acquire first fluorescence spectrum data corresponding to the first ultraviolet laser; responding to an even number of operation instructions acted on the ultraviolet laser, controlling the second ultraviolet laser to emit second ultraviolet laser, and controlling the multichannel acquisition system to acquire corresponding second fluorescence spectrum data;

and the output unit is used for displaying or storing the first fluorescence spectrum data and/or the second fluorescence spectrum data.

A third aspect of the present application discloses a computer device, the device comprising: a memory and a processor; the memory is used for storing program instructions; the processor is configured to invoke program instructions, which when executed, are configured to perform the steps of the method of the first aspect.

A fourth aspect of the present application discloses a computer-readable storage medium, on which a computer program is stored, which computer program, when being executed by a processor, carries out the steps of the method of the first aspect.

A fifth aspect of the present application discloses a computer program product comprising a computer program which, when executed by a processor, implements the steps of the method of the first aspect.

The application has the following beneficial effects:

1. the method and the device creatively use the same PMT array for time-sharing detection, identify event sources by judging parity of for cycle times, realize fluorescence spectrum alternate detection excited by two paths of ultraviolet laser, and realize collection of biological particle multi-mode spectroscopy by cascading breakdown spectrum parts. In addition, when the original data of the dual-band fluorescence spectrum are classified and identified, the high-band fluorescence spectrum data and the low-band fluorescence spectrum data of the same sample can be correspondingly spliced, so that the effective spectrum information of the dual-band fluorescence spectrum is increased, and the accuracy of a classification and identification algorithm of the dual-band fluorescence spectrum can be improved. Similarly, the multi-mode spectrogram combined by the fluorescence spectrum and the breakdown spectrum can increase effective spectrum information, has more characteristic points, can remarkably improve the accuracy of classification and identification results, and can also verify whether the results obtained based on the fluorescence spectrum and the breakdown spectrum are accurate.

2. The application creatively discloses a multi-mode spectroscopy control method for aerosol particles, which is based on multifunctional and automatic control and acquisition software written by a LabVIEW platform, can complete real-time and high-speed multi-mode spectrum data acquisition, display and storage at an industrial personal computer end, and realizes remote control and feedback of a system. The operation flow of the complex system is simplified, and the real-time response and result presentation to the user operation are realized.

3. The application overcomes the current situation that LIF and LIBS are difficult in technology at present, and comprises the steps of increasing the optical paths of a cavity by LIBS during combination, and the requirements and the complexity of time sequences among multiple paths; in addition, the spectrum information obtained after LIF and LIBS are combined is completely different, and the recognition accuracy is remarkably improved.

4. The application creatively discloses a detection device corresponding to an aerosol particle multi-mode spectroscopy control system, which combines a laser-induced fluorescence technology and a laser-induced breakdown spectroscopy technology to realize real-time and rapid discrimination and species identification of biological aerosol, and particularly, the system can rapidly discriminate whether aerosol exists or not through double infrared laser signals; the fluorescence spectrum reflects organic components in the aerosol, and whether the aerosol is biological aerosol can be judged; the breakdown spectrum reflects the composition difference of internal elements among the bioaerosols, so that the high-precision identification of the bioaerosols is realized; the invention can detect and identify bacteria, spores, viruses and toxins in real time in 4 kinds of bioaerosol samples, and carry out coupling analysis based on abundant aerosol particle characteristic spectroscopy parameters, thereby greatly improving the identification capability of bioaerosol early warning and on-site detection equipment on bioaerosol particles, greatly improving the accuracy and depth of data analysis and solving the related life science problems.

Drawings

In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings required for the description of the embodiments will be briefly described below, and it is apparent that the drawings in the following description are only some embodiments of the present invention, and other drawings may be obtained according to these drawings without inventive effort for a person skilled in the art.

FIG. 1 is a schematic flow chart of a method provided in a first aspect of an embodiment of the present invention;

FIG. 2 is a schematic flow chart of a system provided by a second aspect of an embodiment of the present invention;

FIG. 3 is a schematic diagram of the overall structure of a system according to an embodiment of the present invention;

FIG. 4 is a schematic diagram of a computer device provided by an embodiment of the present invention;

FIG. 5 is a schematic view of a sequential structure of an acquisition process according to an embodiment of the present invention;

FIG. 6 is a timing diagram of acquisition process triggers provided by an embodiment of the present invention;

FIG. 7 is a flow chart of the invention for implementing fluorescence spectrum alternate detection by judging parity of for cycle number by using the same PMT array for time-sharing detection;

FIG. 8 is a schematic diagram of a detection device according to an embodiment of the present invention;

FIG. 9 is a schematic diagram of an aerosol generator provided by an embodiment of the present invention;

111. A first infrared laser; 112. a first focusing lens group; 113. a first scattered light receiving path; 114. a first optical point detector; 115. a first voltage amplifier; 116. a second voltage amplifier; 117. a filter; 121. a second infrared laser; 122. a second focusing lens group; 123. a second scattered light receiving path; 124. a second optical point detector; 13. a pulse generator; 141. a first ultraviolet laser; 142. a third focusing lens group; 143. a second ultraviolet laser; 144. a fourth focusing lens group; 145. a first transflective glass; 146. a microobjective; 147. a laser-induced fluorescence signal receiving system; 151. a third infrared laser; 152. a fifth focusing lens group; 153. a laser-induced breakdown spectroscopy signal beam splitting optical path; 154. a spectrometer; 155. triggering a control module; 16. aerosol microbeam; 41. a sample bottle; 42. a storage bin; 43. an ultrasonic vibrator; 44. an air pump; 45. an opening; 46. an air path; 47. a conical nozzle; 48. a chamber.

Detailed Description

In order to enable those skilled in the art to better understand the present invention, the following description will make clear and complete descriptions of the technical solutions according to the embodiments of the present invention with reference to the accompanying drawings.

In some of the flows described in the specification and claims of the present invention and in the foregoing figures, a plurality of operations occurring in a particular order are included, but it should be understood that the operations may be performed out of order or performed in parallel, with the order of operations such as 101, 102, etc., being merely used to distinguish between the various operations, the order of the operations themselves not representing any order of execution. In addition, the flows may include more or fewer operations, and the operations may be performed sequentially or in parallel. It should be noted that, the descriptions of "first" and "second" herein are used to distinguish different messages, devices, modules, etc., and do not represent a sequence, and are not limited to the "first" and the "second" being different types.

The following description of the embodiments of the present invention will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present invention, but not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments according to the invention without any creative effort, are within the protection scope of the invention.

Fig. 1 is a schematic flow chart of a method for controlling multi-modal spectroscopy of aerosol particles according to an embodiment of the present invention, specifically, the method includes the following steps:

101: obtaining a result of whether aerosol to be detected exists in the detection range, and if the aerosol to be detected exists in the detection range, continuing the next step;

in one embodiment, the result of acquiring whether the aerosol to be detected exists in the detection range is obtained by the following ways: responding to a third operation instruction acted on the infrared laser, sending a signal for generating infrared laser by the infrared laser, and irradiating the aerosol micro-beam to be detected by the infrared laser;

102: responding to a first operation instruction acted on the pulse generator, controlling the pulse generator to send a transmitting signal to the ultraviolet laser, and enabling the ultraviolet laser to start transmitting ultraviolet laser, wherein the ultraviolet laser irradiates aerosol microbeam to be detected; specifically, it includes: responding to an odd-number operation instruction acted on the ultraviolet laser, controlling the first ultraviolet laser to emit first ultraviolet laser, and controlling the multichannel acquisition system to acquire first fluorescence spectrum data corresponding to the first ultraviolet laser; responding to an even number of operation instructions acted on the ultraviolet laser, controlling the second ultraviolet laser to emit second ultraviolet laser, and controlling the multichannel acquisition system to acquire corresponding second fluorescence spectrum data;

in one embodiment, the signal of the infrared laser after irradiating the aerosol micro beam to be detected is greater than or equal to the trigger threshold value and is the first operation instruction. When the infrared laser signal is larger than the trigger threshold value, the pulse generator is triggered to start working, and each channel of the pulse generator sequentially triggers the subsequent ultraviolet lasers according to the set delay time sequence (see the time sequence diagram of fig. 6 in detail), so that other operations are not needed.

In one embodiment, the method for determining the odd-numbered operation instruction and the even-numbered operation instruction includes: when the remainder of dividing the current acquisition times by 2 is 0, the current acquisition times are the even number times; when the remainder of dividing the current collection times by 2 is 1, the current collection times are the even number times. Specifically, the odd-even judgment is automatically performed according to the current acquisition times i in the program, for example: the remainder of i=0, 2, 4, 6,i/2 is 0, then the even number is the first number; the remainder of i=1, 3, 5, 7,i/2 is 1, and the odd number is the odd number.

In one embodiment, after said 102, the method further comprises: splicing the first fluorescence spectrum data and the second fluorescence spectrum data to obtain spliced fluorescence spectrum data; displaying or storing the spliced fluorescence spectrum data; the splicing is only to splice two sections of spectrum data end to end into one section, so that the subsequent algorithm data processing is convenient, and no special method is provided. Because the two sections of spectrums respectively correspond to the two paths of lasers, the two paths of lasers cannot be collected at the same time and can only be connected during data processing.

optionally, after the first fluorescence spectrum data is collected, automatically storing the first fluorescence spectrum data; optionally, after the second fluorescence spectrum data is collected, automatically storing the second fluorescence spectrum data;

optionally, the first ultraviolet laser is a 360nm pulse laser; optionally, the second ultraviolet laser is a 266nm pulse laser.

103: displaying or storing the first fluorescence spectrum data and/or the second fluorescence spectrum data;

in one embodiment, the first fluorescence spectrum data and the second fluorescence spectrum data are displayed or saved for convenient viewing by an operator.

In one embodiment, after said 103, the method further comprises: responding to a second operation instruction acted on the infrared pulse laser, controlling the infrared pulse laser to emit third infrared laser, irradiating the biological aerosol microbeam to be detected by the third infrared laser, and controlling a breakdown spectrometer to acquire breakdown spectrum data corresponding to the third infrared laser;

obtaining element types of the bioaerosol microbeam to be detected based on the breakdown spectrum data; optionally, the infrared pulse laser is a 1064nm pulse laser.

in one embodiment, data processing is performed based on the first fluorescence spectrum data, the second fluorescence spectrum data and the breakdown spectrum data to obtain a multi-mode spectrogram; or, performing data processing based on the spliced fluorescence spectrum data and breakdown spectrum data to obtain a multi-mode spectrogram; the fluorescence spectrum and the breakdown spectrum are respectively collected, displayed and stored, and the final multi-mode spectrum is obtained based on the fluorescence spectrum and the breakdown spectrum. The fluorescence spectrum and the breakdown spectrum together form a multi-mode spectrogram.

Further identifying element composition types of the bioaerosol based on the multi-mode spectrogram, and verifying whether a result obtained based on a fluorescence spectrum and a breakdown spectrum is accurate or not; the multi-mode spectrogram combined by the fluorescence spectrum and the breakdown spectrum can increase effective spectrum information, has more characteristic points, and can remarkably improve the accuracy of classification and identification results.

Further, the method for calculating the trigger time of the odd-numbered operation instructions includes: acquiring the speed of an aerosol particle stream to be detected; acquiring a first height difference of infrared laser and first ultraviolet laser; calculating and obtaining the triggering time of a first ultraviolet laser based on the speed and the first height difference, wherein the triggering time of the first ultraviolet laser is the triggering time of the odd-number operation instruction;

optionally, the method for calculating the trigger time of the even number of operation instructions includes: acquiring the speed of an aerosol particle stream to be detected; acquiring a second height difference of the first ultraviolet laser and the second ultraviolet laser; and calculating and obtaining the trigger time of the second ultraviolet laser based on the speed and the second height difference, wherein the trigger time of the second ultraviolet laser is the trigger time of the even-number operation instruction.

In one embodiment, the method for obtaining the height difference includes: from the flow velocity v (ml/s) of the aerosol microbeam and the diameter d (mm) of the aerosol microbeam, the flow velocity of each particle in the aerosol can be calculated to be 4 v/pi d2 (m/s), and the practical factors such as the spot diameter, the chamber height and the like are taken into consideration, wherein the predetermined distance between the excitation points and the pulse delay time are given in a special case: the first height difference between the infrared laser and the first ultraviolet laser is 2mm, and the pulse delay time is 2ms; the second height difference between the first ultraviolet laser and the second ultraviolet laser is 1mm, and the pulse delay time is 1ms.

In one embodiment, the operational instructions include any one or more of the following; mouse click, keyboard input, voice command.

Fig. 2 is a schematic diagram of an aerosol particle multi-modal spectroscopy control system according to an embodiment of the present invention, including:

a judging unit 201, configured to obtain a result of whether an aerosol to be detected exists in the detection range, and if the aerosol to be detected exists in the detection range, continue to the next step;

an interaction unit 202, configured to respond to a first operation instruction applied to the pulse generator, and control the pulse generator to send a transmission signal to the ultraviolet laser, so that the ultraviolet laser starts to transmit ultraviolet laser, and the ultraviolet laser irradiates an aerosol microbeam to be measured; specifically, it includes: responding to an odd-number operation instruction acted on the ultraviolet laser, controlling the first ultraviolet laser to emit first ultraviolet laser, and controlling the multichannel acquisition system to acquire first fluorescence spectrum data corresponding to the first ultraviolet laser; responding to an even number of operation instructions acted on the ultraviolet laser, controlling the second ultraviolet laser to emit second ultraviolet laser, and controlling the multichannel acquisition system to acquire corresponding second fluorescence spectrum data;

And an output unit 203, configured to display or store the first fluorescence spectrum data and/or the second fluorescence spectrum data.

In one embodiment, the system further comprises: acquisition mode selection unit: the method comprises a display-only mode or a display-and-save mode, wherein the selection of the display-only mode automatically collects the spectrum according to the default time sequence setting after the trigger signal is detected and displays the spectrum on a main interface; if the display and storage mode is selected, creating a folder and a corresponding text document in a default path, collecting spectral data and storing the spectral data into the corresponding text document;

optionally, the system further comprises: and the detection unit refreshes the connection state of the equipment, determines the connection state among all the components, and successfully enters the next step. Specifically, the connection state of the equipment is refreshed, the connection states of two groups of infrared continuous lasers, two groups of ultraviolet pulse lasers, one group of infrared pulse lasers, a breakdown spectrometer, a multichannel acquisition system and a delay pulse generator are obtained, the states of the two groups of infrared continuous lasers, the two groups of ultraviolet pulse lasers, the breakdown spectrometer, the multichannel acquisition system and the delay pulse generator are displayed on a main interface through indicator lamps, and if the connection is successful, the corresponding component indicator lamps are on, and if the connection is not successful, the indicator lamps are off.

Optionally, the system further comprises: and initializing a unit.

The following is a main step of one of the embodiments of the present invention, as shown in fig. 3, 5, 6 and 7, and fig. 3 is a schematic diagram of the overall structure of the system provided by the embodiment of the present invention; FIG. 5 is a schematic view of a sequential structure of an acquisition process according to an embodiment of the present invention; FIG. 6 is a timing diagram of acquisition process triggers provided by an embodiment of the present invention;

FIG. 7 is a flow chart of the invention for implementing fluorescence spectrum alternate detection by judging parity of for cycle number by using the same PMT array for time-sharing detection; y is an abbreviation for yes and N is an abbreviation for no;

as shown in fig. 3, the method comprises the following steps: 1. establishing data connection of physical communication through LabVIEW, and providing a receiving and transmitting channel for control and data acquisition commands for each component of the system;

2. designing a LabVIEW sequence structure, and sequentially realizing initial connection state inspection, operation interface instruction input and response, spectrum data acquisition record and display of each component according to the design sequence;

3. the initial connection state of each component is checked to be the connection state of 780nm/850nm continuous laser, 360nm/266nm pulse laser, 1064nm pulse laser, breakdown spectrometer, multichannel acquisition system and delay pulse generator. The initialization process includes all laser shutters remaining closed; the integration time, the integration delay, the acquisition mode and the triggering mode of the acquisition system are configured to be default values; the parameters of each channel of the delay pulse generator are configured to be default values.

The LabVIEW sequence structure comprises:

the first step: checking and initializing connection states of two groups of infrared continuous lasers, two groups of ultraviolet pulse lasers, one group of infrared pulse lasers, a breakdown spectrometer, a multichannel acquisition system and a delay pulse generator;

and a second step of: the method comprises the steps of realizing instruction input and response and data acquisition of a system operation interface through an event structure, performing data processing to obtain a multi-mode spectrogram, and displaying a result on a software main interface in real time;

and a third step of: and automatically storing data after completing data acquisition and recording, and waiting for a new instruction.

After the initialization process is finished, the main processing cycle of the program is entered, an event structure is built in the main processing cycle, corresponding to 8 events in the main processing cycle respectively, wherein,

event 0, as shown in FIG. 5, refreshes the device connection state; event 1, starting acquisition/stopping acquisition; an event 2, setting parameters of an ultraviolet laser; an event 3, setting parameters of an infrared laser; event 4, pulse generator parameter setting; 5, collecting system parameter setting; event 6.Libs laser parameters settings; and 7, exiting the program.

And the connection state of the equipment is refreshed, so that the connection states of two groups of infrared continuous lasers, two groups of ultraviolet pulse lasers, one group of infrared pulse lasers, a breakdown spectrometer, a multichannel acquisition system and a delay pulse generator are obtained, the states of the two groups of infrared continuous lasers, the two groups of ultraviolet pulse lasers, the breakdown spectrometer, the multichannel acquisition system and the delay pulse generator are displayed on a main interface through indicator lamps, the corresponding component indicator lamps are on when the connection is successful, and the indicator lamps are off when the connection is not successful.

The method comprises the following steps that 1, acquisition/stopping acquisition is started, the events are based on a sequence structure and a selection structure, and in an acquisition mode selection module, if a 'display only mode' is selected, a spectrum is automatically acquired according to a default time sequence after a trigger signal is detected, and the spectrum is displayed on a main interface; if the display and storage mode is selected, creating a folder and a corresponding text document in a default path, collecting spectral data and storing the spectral data into the corresponding text document; in order to realize the alternate detection of fluorescence excited by two paths of ultraviolet laser, the data acquisition process identifies the source of an event by judging the parity of the for cycle times, namely, the odd-numbered acquisition of fluorescence excited by the corresponding first path of ultraviolet laser and the even-numbered acquisition of fluorescence excited by the corresponding second path of ultraviolet laser are considered; and for the breakdown spectrum module, the breakdown spectrum excited by the infrared pulse laser is acquired once per cycle. Because of the fast aerosol microbeam flow rate, to reduce the time interval of two LIF channels, the event data collected each time is temporarily stored in a temporary array. After the fluorescence spectrum data and the breakdown spectrum data of the two channels are collected, data processing is conducted in a centralized mode, and the data are displayed in a drawing mode and written into files generated in a magnetic disk.

The specific acquisition process is as follows: firstly, the user can select the acquisition mode to be a 'display only mode' or a 'display and save mode' (the default setting is a display and save mode), and the energy of each laser, the voltage value of the PMT, the integration time of the acquisition system and the like can be adjusted according to the requirement (the default value is enough in general). In the display and save mode, after the acquisition is started, the system creates a folder and a corresponding text document in a default path, and opens a 780nm/850nm infrared continuous laser to detect whether a scattered signal exists. If the scattering signal is detected, triggering the delay pulse generator to start working, and triggering the 360nm/266nm pulse laser, the multichannel acquisition system, the 1064nm pulse laser and the breakdown spectrometer in sequence according to a set time sequence, displaying each spectrum curve in a software main interface, and storing the spectrum curves in a corresponding text document.

The default timing is set to: when the two paths of infrared elastic scattering signals reach a set threshold value, the system outputs a path of trigger signal to the pulse generator, and the pulse generator controls to close the two paths of infrared lasers through negative polarity output, wherein the two paths of infrared elastic scattering signals represent that particles enter a detection range. According to the speed of aerosol particle flow and the height difference between infrared light and the first path of ultraviolet laser, the triggering time of the first path of ultraviolet laser can be calculated. Similarly, according to the speed of aerosol particle flow and the height difference between the first path of ultraviolet laser and the second path of ultraviolet laser, the triggering time of the second path of ultraviolet laser can be calculated, and the dual-band fluorescence spectrum detection is completed. After the computer stores the two fluorescence spectrums, triggering the infrared pulse laser to detect the breakdown spectrums, and triggering the breakdown spectrometer to collect and store the breakdown spectrums. At this point, the entire cycle is completed, and the two infrared continuous lasers are reset and turned on again and ready to accept the next trigger acquisition.

As shown in fig. 6, the predetermined timing sequence of the delay pulse generator is: when the scattered signal passes through the amplifying and filtering circuit and exceeds a trigger threshold, the scattered signal is detected, the pulse generator channel 1 controls the two infrared lasers for scattering positioning to close light through negative polarity output, and Deltat 1 is the insertion delay of the electric signal processing module; the channel 2 simultaneously controls the light emission of the 360nm laser and the spectrum collected by the collecting system, and the excitation and collection duration t1 of the low-band fluorescence spectrum; delta t2 is the interval from the first LIF point to the second LIF point of the particle beam, and delta t2 can be calculated according to the speed of the aerosol particle stream and the height difference of the two points, so that after the interval delta t2, a channel 3 simultaneously controls the light emission of a 266nm laser and the acquisition spectrum of an acquisition system, and the excitation and acquisition duration t2 of a high-band fluorescence spectrum; delta t3 is the time for collecting and recording fluorescence spectrum, and the channel 4 simultaneously controls the light emission of the 1064nm laser and the spectrum collected by the breakdown spectrometer after the interval Delta t 3; deltat 4 is the computer data processing and storing time; until the system completes the collection, processing and storage of the two fluorescence spectra and the breakdown spectrum, at this time, the whole cycle is ended, and the infrared laser is reset and ready to accept the next trigger. The period of the dual infrared laser signal is the overall working period of the system.

As shown in fig. 7, a flow chart of a method for distinguishing the spectrum data corresponding to the 360nm laser and the 266nm laser for the acquisition system is shown: in order to realize the alternate detection of fluorescence excited by 360nm and 266nm, the same PMT array is used for time-sharing detection in the data acquisition process, and the event source is identified by judging the parity of for cycle times, namely, the fluorescence data excited by 266nm is acquired in the odd number and the fluorescence data excited by 360nm is acquired in the even number.

The software corresponding to the block diagram is written in LabVIEW language and used for collecting setting, data transmission and data storage, and the software issues instructions to all components through serial ports, and comprises two groups of infrared continuous lasers, two groups of ultraviolet pulse lasers, one group of infrared pulse lasers, a fluorescence spectrometer, a breakdown spectrometer, a multichannel collecting system, a time delay pulse generator and the like. The multi-mode spectrum data can be collected, displayed and stored at high speed in real time at the industrial personal computer through the multi-functional and automatic control and collection software, remote control and feedback of the system are realized, and meanwhile, the multi-mode spectrum data are preprocessed in real time and displayed graphically and are synchronously stored in the computer in the form of text documents. The operation flow of the complex system is simplified, the real-time response and result presentation to the user operation are realized, and basic guarantee is provided for the detection system work.

FIG. 8 is a schematic diagram of a detection device for software control management according to an embodiment of the present invention; specifically, the device comprises: a chamber 48 for containing aerosol beamlets 16 formed by a stream of aerosol particles to be measured;

in some embodiments, the aerosol particle stream to be measured is generated by an aerosol generating module. The aerosol generating module comprises: a chamber 48 supported on the chamber 48 support to accommodate the aerosol microbeam 16 to be measured;

an aerosol generator, comprising: a storage bin 42 that stores a sample to be measured; an ultrasonic vibrator 43 connected to the storage bin 42 to atomize a substance to be measured to form an aerosol; an air pump 44 which generates compressed air and passes clean air into the aerosol generator; a gas circuit 46 connecting the aerosol generator and the chamber 48, respectively, to deliver a uniform aerosol spray generated by the aerosol generator to the chamber 48; a conical orifice 47 which forms a uniform and stable flow of aerosol particles. Specifically, as shown in fig. 9, fig. 9 is a schematic diagram of an aerosol generator according to an embodiment of the present invention, which includes a sample bottle 41 having a storage bin 42, and a sample to be measured is stored in the storage bin 42; the lower end of the ultrasonic vibrator 43 extends into the sample to be tested in the storage bin 42, and the upper end of the ultrasonic vibrator 43 is connected with the air pump 44 through the air channel 46; an opening 45 connected with a gas path 46 is arranged on one side of the sample bottle 41, uniform aerosol spray generated by a sample to be tested enters the cavity 48 through the opening 45 and the gas path 46, and a conical nozzle 47 for forming uniform and stable aerosol particle flow by spraying is arranged on the wall of the cavity 48. The opening 45 is located above the storage bin 42, i.e. the sample to be measured cannot flow out of the sample bottle 41 through the opening 45.

The dual-infrared laser positioning triggering module is used for determining the instantaneous position of the aerosol particle stream to be detected and providing a triggering signal;

in some specific embodiments, the dual infrared laser positioning trigger module comprises: the first infrared laser 21 positioning triggering module and the second infrared laser 22 positioning triggering module are used for generating first infrared laser 21 with a first wavelength, receiving infrared scattered light of the first infrared laser 21 after being scattered by the aerosol microbeam 16, and forming a first excitation point by the first infrared laser 21 through the aerosol microbeam 16; the second infrared laser 22 positioning triggering module is configured to generate a second infrared laser 22 with a second wavelength, and receive infrared scattered light that is backscattered by the second infrared laser 22 by the aerosol microbeam 16, where the second infrared laser 22 forms a second excitation point by the aerosol microbeam 16; the first excitation point and the second excitation point are coincided; the infrared laser light is directed into the chamber 48 at a first angle to the direction of incidence of the aerosol microbeam 16. The first included angle is 10-90 degrees; the arrangement of the first excitation point and the second excitation point which are coincident can determine that the same particle is excited, and the crossed infrared light paths of the first included angle can form an overlapped excitation area so as to better position the microbeam.

Still further, the first infrared laser 21 positioning triggering module includes: a first infrared laser 111 for outputting a first infrared laser light 21 having a first wavelength; a first focusing lens group 112 for receiving and focusing the first infrared laser light 21, wherein a focal point of the first infrared laser light 21 forms a first excitation point on the aerosol microbeam 16; a first scattered light receiving path 113 for receiving first infrared scattered light scattered by the first excitation point; the filter comprises a first band-pass filter corresponding to a first wavelength and a first band-stop filter corresponding to a second wavelength;

still further, the second infrared laser 22 positioning triggering module includes: a second infrared laser 121 for outputting a second infrared laser light 22 having a second wavelength; a second focusing lens group 122 for receiving and focusing the second infrared laser light 22, the focal point of the second infrared laser light 22 forming a second excitation point on the aerosol microbeam 16; a second scattered light receiving path 123 for receiving second infrared scattered light scattered by the first excitation point, including a second bandpass filter corresponding to a second wavelength and a second bandstop filter corresponding to the first wavelength; preferably, before the first focusing lens group 112, a fiber coupling lens is disposed after the first infrared laser 111, and similarly, before the second focusing lens group 122, a fiber coupling lens is disposed after the second infrared laser 121.

Further, the first infrared laser 21 positioning triggering module further includes a first optical point detector 114, which receives the first infrared scattered laser light for gain amplification; the second infrared laser 22 positioning triggering module further comprises a second optical point detector 124, which receives the second infrared scattered laser light for gain amplification; the first photodetector and the second photodetector may be PINs or PMTs, as the case may be.

Further, the dual-infrared laser positioning triggering module further comprises an amplifying and filtering circuit module, wherein the amplifying and filtering circuit module amplifies and filters the electric signals received by the first photoelectric detector and the second photoelectric detector to form a triggering signal so as to trigger the subsequent module.

The double ultraviolet laser induction fluorescence module starts to operate after receiving the trigger signal, and comprises:

the double ultraviolet laser emission module is used for generating at least two paths of ultraviolet pulse lasers with different wave bands; the dual ultraviolet laser emission module comprises a first half-transparent half-reflective glass 145, the first half-transparent half-reflective glass 145 faces the cavity 48 and respectively transmits and reflects optical signals according to wavelengths to integrate ultraviolet pulse laser light paths of the at least two paths of different wave bands, the integrated ultraviolet pulse laser light is injected into the cavity 48 to form different detection points on the aerosol microbeam 16, and aerosol particle flows are excited to generate different signals; a second included angle is formed between the direction of the ultraviolet pulse laser beam entering the cavity 48 and the entering direction of the aerosol microbeam 16; the second included angle is 90 degrees. Here we use semi-transparent semi-reflective lens to meet the requirement of transmitting and reflecting one of the two ultraviolet pulse lasers.

In some specific embodiments, the different detection points include a first detection point and a second detection point formed by a first ultraviolet pulse laser and a second ultraviolet pulse laser on the aerosol microbeam 16 to be detected, and the first ultraviolet pulse laser and the second ultraviolet pulse laser excite the bioaerosol particle stream to generate a first signal and a second signal; at this time, the first half-transmitting and half-reflecting glass 145 transmits and reflects the optical signals according to the wavelengths to integrate the first uv pulse laser path and the second uv pulse laser path, and the first uv pulse laser and the second uv pulse laser form a first detection point and a second detection point on the aerosol microbeam 16 to be detected;

in some embodiments, the dual ultraviolet laser emitting module further comprises: a first ultraviolet laser 141 for outputting a first ultraviolet pulse laser light having a third wavelength; a third focusing lens group 142 for receiving and focusing the first ultraviolet pulse laser light; a second ultraviolet laser 143 for outputting a second ultraviolet pulse laser light having a fourth wavelength; and a fourth focusing lens group 144 for receiving and focusing the second ultraviolet pulse laser light.

The double ultraviolet laser-induced fluorescence receiving module is used for receiving the different signals (the first signal and the second signal) and generating a fluorescence spectrum; and obtaining a result of whether the aerosol to be detected is a biological aerosol or not based on the fluorescence spectrum.

In some embodiments, the dual ultraviolet laser-induced fluorescence receiving module comprises: microscope objective 146 comprising a concave mirror receiving a first and a second signal and a convex mirror opposite to said concave mirror to converge the first and second signals; the laser-induced fluorescence signal receiving system 147 is used for receiving and processing the first signal and the second signal. Depending on the particular needs, either a photodetector or spectrometer 154 may be used.

In some embodiments, the system further comprises a laser-induced breakdown module that is turned on when the aerosol to be tested is obtained as a result of a bioaerosol; the laser-induced breakdown module is used for generating infrared laser with a fifth wavelength, receiving signals generated by exciting the bioaerosol particle stream by the infrared laser with the fifth wavelength, and generating a breakdown spectrum after processing the signals; the type of bioaerosol is identified based on the breakdown spectrum.

In some specific embodiments, the laser-induced breakdown module comprises: a third infrared laser 151 for outputting a third infrared laser light of a fifth wavelength; a fifth focusing lens group 152, configured to receive and focus the third infrared laser light, where a focal point of the third infrared laser light is focused to form a third detection point on the aerosol microbeam 16, and the third detection point and the second detection point are vertically spaced from a predetermined distance, and the third infrared laser light excites the bioaerosol particle stream to generate a third signal; the laser-induced breakdown spectroscopy signal beam splitting optical path 153 is configured to split, filter and focus the third signal according to a band; the laser beam splitting optical path converts the interaction between the sample and the light source into a measurable optical signal by utilizing the characteristics of high brightness, monochromaticity and straightness emitted by the laser light source. A spectrometer 154 for receiving the third signal to generate a breakdown spectrum; the third infrared laser 151 includes a fifth focusing lens group 152 and a breakdown spectroscopy signal beam path.

In some embodiments, the system further comprises: a trigger control module 155 and a processing unit; the trigger control module 155 is connected with the dual-infrared laser positioning trigger module, and when aerosol particles reach a first excitation point and a second excitation point, the trigger control module 155 sends out a trigger signal; the processing unit is connected with the trigger control module 155, the double-infrared laser positioning trigger module, the double-ultraviolet laser-induced fluorescence module and the laser-induced breakdown module; the processing unit responds to the trigger signal and sends an interrupt signal to the dual infrared laser positioning trigger module to stop emitting the first infrared laser 21 and the second infrared laser 22, and simultaneously sends an emission signal to the dual ultraviolet laser induced fluorescence module to emit the first ultraviolet laser 23 and the second ultraviolet laser 24 and sends an emission signal to the laser induced breakdown module to emit the third infrared laser.

Fig. 4 is a computer device according to an embodiment of the present invention, where the device includes: a memory and a processor; the memory is used for storing program instructions; the processor is configured to invoke program instructions, which when executed, are configured to perform the steps of the method described above.

The embodiment of the invention also provides a computer readable storage medium, on which a computer program is stored, which when being executed by a processor, implements the steps of the method described above.

The embodiment of the invention also discloses a computer program product, which comprises a computer program, wherein the computer program realizes the steps of the method when being executed by a processor.

Definition:

LabVIEW is a program development environment similar to the C and BASIC development environments, but the LabVIEW differs significantly from other computer languages in that: other computer languages all use text-based languages to generate code, while LabVIEW uses the graphical editing language G to write programs, the generated programs being in block form.

LabVIEW software is the core of the NI design platform and is also an ideal choice for developing measurement or control systems. The LabVIEW development environment integrates all the tools required by engineers and scientists to quickly build a variety of applications, aiming at helping engineers and scientists solve problems, improve productivity and continue to innovate.

The laser-induced fluorescence (Laser Induced Fluorescence, LIF) is a spectroscopic technique that uses laser as an excitation source to excite a substance to be measured to generate fluorescence and detect; the fluorescence spectra of different aerosol particles are detected by a laser-induced fluorescence technology, and the difference of fluorescence intensities of different wave bands is analyzed, so that the organic particles and the inorganic particles in the aerosol particles can be distinguished. At present, the method is widely applied to various fields such as animal and plant detection, food detection, mineral detection and pollutant detection. The laser-induced fluorescence technology belongs to photoluminescence, under the excitation of laser with specific wavelength, the energy of a molecule absorption photon transits from a ground state to an excitation state, the molecule in the excitation state is unstable, the lowest vibration energy level of a first excitation state is transited back through modes such as vibration relaxation, conversion and the like, the transition to the ground state is continued after a short stay, and the energy is released in the form of an emission photon when the molecule transits to the ground state, and the process is fluorescence emission. Photon energy generated in the fluorescence emission process is energy difference between the vibration energy of the corresponding excited state and the ground state, so that the transition of molecules from different energy levels to the ground state correspondingly emits photons with different frequencies, and fluorescence signals with different wavelengths are generated. For organic matters, the fluorescence spectrum is formed by superposing the fluorescence spectra of various intrinsic fluorescent groups in the organic matters, wherein the fluorescent groups mainly comprise aromatic amino acids, nicotinamide adenine dinucleotide, flavin, pyridoxine derivatives, chlorophyll and the like, the optimal excitation wavelength of the aromatic amino acids such as tryptophan, tyrosine, phenylalanine and the like is 260-295nm, and the fluorescence spectrum emission range is 280-360nm; the optimal excitation wavelength and the optimal emission wavelength of NADH are 340nm and 460nm respectively; the optimal excitation wavelength of coenzyme substances such as riboflavin, flavin mononucleotide, flavin adenine dinucleotide and the like is 450nm, and the optimal fluorescence emission wavelength is 525nm. Most inorganic particles do not contain the fluorescent groups, and can not emit fluorescent signals of corresponding wave bands. Therefore, the fluorescence spectra of different aerosol particles are detected by a laser-induced fluorescence technology, and the difference of fluorescence intensities of different wave bands is analyzed, so that the organic particles and the inorganic particles in the aerosol particles can be distinguished.

Laser Induced Breakdown Spectroscopy (LIBS) is an analytical technique currently under development for rapid, on-site quantitative detection of elemental constituents in samples. The method has the technical advantages of long-distance detection, small destructiveness, high sensitivity, low detection limit, simultaneous multi-element analysis and the like, and has great application potential in various industries such as social industrial application, environmental engineering, deep space exploration, cultural relic protection, medical treatment and the like. LIBS is based on the one-to-one correspondence between the wavelengths of atomic spectrum and ion spectrum and specific elements, and the spectrum signal intensity and the content of the corresponding elements also have a certain quantitative relationship, laser is focused on the surface of a sample through a lens, and when the energy density of laser pulse is greater than the breakdown threshold of a substance to be detected, plasma is generated by excitation, which is called laser-induced plasma. The plasma is gradually cooled as it expands towards the external environment and emits elemental composition information of the sample to be measured, and the emission spectrum of the plasma is collected using a photodetector and spectrometer 154. The element information and the content information of the sample to be detected are further obtained by analyzing the plasma spectrum and combining a qualitative and quantitative analysis model, so that the accurate classification of the bioaerosol particles is realized.

An aerosol containing biological particles. Including bacteria, viruses, sensitized pollen, mould spores, fern spores, parasitic ova and the like, and has infectivity, sensitization and the like in addition to the characteristic of common aerosol. Aerosol refers to a gaseous dispersion system of solid or liquid particles suspended in a gaseous medium. Among them, liquid small particles constitute clouds, fog, etc., solid small particles constitute smoke, haze, etc., and if they are mixed with bacteria or viruses inside, such aerosols with virus bacteria are called bioaerosols, and common bioaerosols include bacteria, spores, viruses, mildews, etc. Aerosols are ubiquitous and pore-free in daily life, and are closely related to human life health.

The results of the verification of the present verification embodiment show that assigning an inherent weight to an indication may moderately improve the performance of the present method relative to the default settings.

It will be clear to those skilled in the art that, for convenience and brevity of description, specific working procedures of the above-described systems, apparatuses and units may refer to corresponding procedures in the foregoing method embodiments, which are not repeated herein.

In the several embodiments provided in this application, it should be understood that the disclosed systems, apparatuses, and methods may be implemented in other ways. For example, the apparatus embodiments described above are merely illustrative, e.g., the division of the units is merely a logical function division, and there may be additional divisions when actually implemented, e.g., multiple units or components may be combined or integrated into another system, or some features may be omitted or not performed. Alternatively, the coupling or direct coupling or communication connection shown or discussed with each other may be an indirect coupling or communication connection via some interfaces, devices or units, which may be in electrical, mechanical or other form.

The units described as separate units may or may not be physically separate, and units shown as units may or may not be physical units, may be located in one place, or may be distributed on a plurality of network units. Some or all of the units may be selected according to actual needs to achieve the purpose of the solution of this embodiment.

In addition, each functional unit in the embodiments of the present invention may be integrated in one processing unit, or each unit may exist alone physically, or two or more units may be integrated in one unit. The integrated units may be implemented in hardware or in software functional units.

Those of ordinary skill in the art will appreciate that all or part of the steps in the various methods of the above embodiments may be implemented by a program to instruct related hardware, the program may be stored in a computer readable storage medium, and the storage medium may include: read Only Memory (ROM), random access Memory (RAM, random Access Memory), magnetic or optical disk, and the like.

Those of ordinary skill in the art will appreciate that all or a portion of the steps in implementing the methods of the above embodiments may be implemented by a program to instruct related hardware, where the program may be stored in a computer readable storage medium, where the storage medium may be a read only memory, a magnetic disk or optical disk, etc.

While the foregoing describes a computer device provided by the present invention in detail, those skilled in the art will appreciate that the foregoing description is not meant to limit the invention thereto, as long as the scope of the invention is defined by the claims appended hereto.

Claims

1. A method of multi-modal spectroscopy control of aerosol particles, the method comprising:

2. The method of claim 1, wherein the method of determining the odd-order and even-order operation instructions comprises: when the remainder of dividing the current acquisition times by 2 is 0, the current acquisition times are the even number times; when the remainder of dividing the current collection times by 2 is 1, the current collection times are the even number times.

3. The aerosol particle multi-modal spectroscopy control method of claim 1, wherein after S2, the method further comprises: splicing the first fluorescence spectrum data and the second fluorescence spectrum data to obtain spliced fluorescence spectrum data; displaying or storing the spliced fluorescence spectrum data;

Optionally, the first ultraviolet laser is a 360nm pulse laser;

optionally, the second ultraviolet laser is a 266nm pulse laser.

4. A method of multi-modal spectral control of aerosol particles according to claim 3, wherein after S3 the method further comprises: responding to a second operation instruction acted on the infrared pulse laser, controlling the infrared pulse laser to emit third infrared laser, irradiating the biological aerosol microbeam to be detected by the third infrared laser, and controlling a breakdown spectrometer to acquire breakdown spectrum data corresponding to the third infrared laser;

optionally, the infrared pulse laser is a 1064nm pulse laser.

5. The aerosol particle multi-modal spectroscopic control method of claim 4, wherein the multi-modal spectrogram is obtained by performing data processing based on the first fluorescence spectrum data, the second fluorescence spectrum data, and the breakdown spectrum data; or, performing data processing based on the spliced fluorescence spectrum data and breakdown spectrum data to obtain a multi-mode spectrogram;

6. The method of claim 5, wherein the method of calculating the trigger time of the odd-numbered operation instructions comprises:

acquiring the speed of an aerosol particle stream to be detected;

7. The aerosol particle multi-modal spectroscopy control method of claim 1, wherein the operating instructions comprise any one or more of the following; mouse click, keyboard input, voice command.

8. An aerosol particle multi-modal spectroscopy control system, the system comprising:

9. A computer device, the device comprising: a memory and a processor; the memory is used for storing program instructions; the processor being adapted to invoke program instructions, which when executed, are adapted to carry out the steps of the method according to any of claims 1-7.

10. A computer-readable storage medium, characterized in that a computer program is stored thereon, which computer program, when being executed by a processor, realizes the steps of the method of any of the preceding claims 1-7.