CN205719979U - In a kind of liquid, induced with laser strengthens detection and the sorting unit of Raman spectrum - Google Patents
In a kind of liquid, induced with laser strengthens detection and the sorting unit of Raman spectrum Download PDFInfo
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- CN205719979U CN205719979U CN201620295251.1U CN201620295251U CN205719979U CN 205719979 U CN205719979 U CN 205719979U CN 201620295251 U CN201620295251 U CN 201620295251U CN 205719979 U CN205719979 U CN 205719979U
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Abstract
The utility model discloses induced with laser in a kind of liquid and strengthen detection and the sorting unit of Raman spectrum.This utility model focuses on the testing sample of suspension by induction light, forms three-dimensional ligh trap in a liquid, and enriched in metals nano-particle is in the surface of testing sample, is overlapped with induction optical focus by exciting light, forms induced with laser and strengthens Raman signal；Implementation method of the present utility model is easy, and spectrum reinforced effects is obvious, and the biological sample in-situ Raman spectrum being suitable in liquid environment quickly detects；Need testing sample jointly to hatch with metal nanoparticle, be particularly well-suited to the field of fast detection such as disease, epidemic situation, food safety；Greatly reduce the metal nanoparticle toxic damages to biological sample, it is thus possible to farthest reaction living cells actual biological state；The optical tweezer that this utility model is formed can carry out sorting, extracting and eluting, it is achieved the follow-up study of the Raman spectrum identification of single living cell and sorting function, beneficially biological sample.
Description
Technical field
This utility model relates to Raman signal sample detection technology, is specifically related to induced with laser in a kind of liquid and strengthens detection and the sorting unit of Raman spectrum.
Background technology
When light beam is irradiated on sample, its scattered light overwhelming majority photon is with the form outgoing of Rayleigh scattering, and its scattered light is equal with incident light frequency.Additionally, the photon that there is more few part will occur Raman scattering, energy exchange is produced with sample when these photons interact, the frequency of emergent light can occur blue shift or red shift, being referred to as Raman shift (Raman Shift), both correspond respectively to anti-Stokes (anti-Stokes) line and Stokes (Stokes) line.Practical study generally collects Raman diffused light with spectrogrph and forms Raman spectrum.The FLUORESCENCE STUDY method the most commonly used relative in biological study, the most prominent advantage of Raman spectrum is non-marked, the narrowest without photobleaching, spectral line, susceptiveness is high, abundant information, can be described as " molecular fingerprint identification technology " with the chemical constituent of quantitative response sample and content.And normal Raman scattered signal is the faintest, it is research, the maximum restraining factors of application development.
For the problem that Raman signal is the most weak, at present, the method having developed a series of enhancing Raman signal, specifically include two classes.The first kind is based on local surface plasma resonance (Localized Surface Plasmon Resonance, LSPR) surface-enhanced Raman (the Surface Enhanced Raman Spectroscopy of effect, SERS) and point strengthen Raman (Tip Enhanced Raman Spectroscopy, TERS).According to strengthening principle, testing sample needs there is the nearest physical distance with the base material that can produce plasma effect.So SERS, TERS are often combined with ultramicroscope realizes Raman signal enhancing under vacuum conditions, more to inorganic samples researchs such as material sciences.The method is very limited for the research of the most all biological field samples in water environment.In recent years, developed a series of Raman optical spectrum method based on SERS principle accordingly and carried out the samples such as on-spot study microorganism, made some progress [1,2].At present, utilizing SERS to carry out biological sample research, topmost way of realization is to be still to be placed in bottom sample by SERS sensitivity nano-particle with the form of substrate, it is impossible to the biological activity of sample is effectively ensured.
The enhancing of Equations of The Second Kind Raman signal depends on nonlinear optical effect, specifically include: resonance raman (Resonance Raman Spectroscopy, RRS), excited Raman (Stimulated Raman Spectroscopy, SRS), coherent anti-Stokes Raman (Coherent anti-Stocks Raman Spectroscopy, CARS).This type of strengthens mainly dependence ultrafast laser system and realizes, it is achieved process is relative complex, requires the highest to instrument hardware, experimental implementation.
At present, method most like with this utility model in utilizing SERS technology to study biological sample is the gold with colloidal form or silver nano-grain and the sample such as biological is directly mixed into solution, then dripped to measure in substrate sheet, to be dried after directly measure.Conventional substrate has calcium fluoride, silicon, aluminium flake etc..The parameter that experiment changes generally comprises nano-particle colloid size, particle aggregation form etc..Can guarantee that testing sample and nano-particle have the nearest physical distance to reach the effect strengthened after drying.The maximum feature of the method is that to realize process easy, and reinforced effects is most important to consider standard.
Metallic particles (thin layer) is directly synthesized in sample surfaces or cell wall, is another kind of SERS Enhancement Method.Synthesize various types of nano-particle, nanoscale ditch (gap) strengthens LSPR effect, or granule carries out three-dimensional group is filled with the contact area expanding sample and metal surface.Additionally, synthesis metal nanoparticle " is spread " on sample；Watchcase at nano-metal particle pan coating good biocompatibility；Jointly hatch with biological sample and cultivate to realize the SERS method that endocytosis etc. is all development recently.
Utility model content
For above problems of the prior art, the utility model proposes induced with laser in a kind of liquid and strengthen detection and the sorting unit of Raman spectrum.
In liquid of the present utility model, the detection of induced with laser enhancing Raman spectrum includes with sorting unit: induction electro-optical device, excitation-light unit, light combination mirror, the first dichroic mirror, object lens, sample cell, metal nanoparticle, condenser lens, exciting light wave filter, Raman spectrometer and computer；Wherein, testing sample uniformly mixes with metal nanoparticle, is dissolved in the liquid being contained in transparent sample cell；Induction electro-optical device sends near-infrared laser as induction light, through light combination mirror, object lens are entered again through the first dichroic mirror, by the object lens high order focusing of high-NA, focus is positioned on the testing sample being suspended in sample cell, forming three-dimensional ligh trap in a liquid, suspend fixing testing sample, and enriched in metals nano-particle is in the surface of testing sample；Excitation-light unit sends the laser of visible light wave range, as exciting light, after light combination mirror with induction combiner, object lens are entered through the first dichroic mirror, by the object lens high order focusing of high-NA, focus is similarly positioned on the testing sample in sample cell, forms induced with laser and strengthens Raman signal；After induced with laser enhancing Raman signal is focused on by object lens, through the first dichroic mirror and exciting light and induction light branch, through exciting light wave filter, then after line focus lens focus, enter Raman spectrometer, obtain induced with laser and strengthen Raman spectrum；Raman spectrometer is connected to computer through data wire, analyzes induced with laser enhancing Raman spectrum and obtains the information of testing sample.
Sample cell uses non-blooming transparent material.Holding the sample being dissolved in liquid in sample cell, testing sample includes biological sample and abiotic particulate matter；If testing sample is one or more in cell, albumen, microorganism and chemical molecular, liquid uses the one in buffer, culture fluid and aqueous solution；If testing sample is the solid particulate matter in air, then liquid is aqueous solution or organic solvent.Coverslip is set at the top of sample cell, for covering liquid, to reduce liquid-to-air contact exchange, increases the stability of testing result, especially in volatility detects, effectively reduce error.
The material of metal nanoparticle uses the one in the coinage metal such as gold, silver, platinum and copper, laser excitation local surface plasma resonance LSPR effect.The incident wavelength of laser is less than 100nm more than the diameter 10 times of metal nanoparticle, the diameter of metal nanoparticle.Induction light and exciting light the most likely excite metal nanoparticle to produce local surface plasma resonance LSPR.
Induction electro-optical device includes: near infrared laser, the first electronic shutter and coupled lens group；Wherein, near infrared laser sends near-infrared laser, and through the first electronic shutter, coupled lens group after expanding, the diameter of light beam, more than the diameter of the rear pupil of object lens, forms induction light.First electronic shutter, as the switch of induction electro-optical device, controls the switch of near infrared laser, thus uses laser to carry out the time etc. operated when controlling to induce the induction time of light, elution separation.
Excitation-light unit includes: TEM00 mode laser, the second electronic shutter and beam expanding lens group；Wherein, solid state laser sends the laser of visible light wave range, beam expanding lens group expand, and forms exciting light, arranges the second electronic shutter in beam expanding lens group.Second electronic shutter, as the switch of excitation-light unit, controls the switch of solid state laser, thus controls the Raman scattering firing time of exciting light.First electronic shutter and the second electronic shutter are by remote controller Non-follow control.
Further, this utility model also includes illumination light device and imaging device, arranges illumination light device in the side that sample cell is the most relative with object lens, and outward and object lens homonymy arranges imaging device at sample cell；Illumination light device launches bright field light, it is radiated in sample cell, transmission light is after object lens focus on, through the first dichroic mirror and induction light branch, Raman signal branch is strengthened with induced with laser again through the second dichroic mirror, being imaged on imaging device, imaging device is connected to monitor through data wire, in real time display testing sample within sample cell.Imaging device is connected to computer by data wire, it is achieved the control to imaging device.Imaging device uses CCD camera.
Illumination light device launches bright field light, it is radiated in sample cell, after object lens focus on, through the first dichroic mirror and induction light branch, Raman signal branch is strengthened with induced with laser again through the second dichroic mirror, it is imaged on imaging device, and by image transmitting to monitor, display testing sample within sample cell in real time；The focus controlling induction light is positioned on selected sample, after the three-dimensional ligh trap inducing light to be formed in a liquid is stable, control the movement of the mobile drive sample cell of object stage, thus the three-dimensional ligh trap relative sample pond controlling to induce light to be formed is moved, make sample and the sample cell generation relative movement selected, it is achieved sample sorting and extraction.
Fixing a capillary tube in sample cell, the internal diameter of capillary tube is placed in the sample slightly larger than the size of selected sample, one end of capillary tube, and the other end is connected with micro-syringe；When selected sample moves to capillary tube port in sample cell, syringe applies negative pressure, by selected sample sucking-off.Before sorting, also including eluting: control induction light motionless, three ligh trap constraint suspensions that testing sample is induced to be formed are fixing in the solution, sample cell carried out perfusion wash, it is achieved the eluting of metal nanoparticle.
Advantage of the present utility model:
This utility model focuses on the testing sample of suspension by induction light, form three-dimensional ligh trap in a liquid, metal nanoparticle is made to be enriched in the surface of testing sample, overlapped with induction optical focus by exciting light, common effect, excite LSPR and Raman signal, Raman signal and LSPR to interact simultaneously and produce induced with laser enhancing Raman signal reinforced effects, thus the more convenient information obtaining testing sample；Implementation method of the present utility model is easy, and spectrum reinforced effects is obvious, and the biological sample in-situ Raman spectrum being suitable in liquid environment quickly detects；This utility model need not testing sample and jointly hatches with metal nanoparticle, can be effectively improved conventional efficient, reduces consumptive material consumption, is particularly well-suited to the field of fast detection such as disease, epidemic situation, food safety；Simultaneously as enhancement effect, intensity and the Spectral acquisition times of exciting light can be effectively reduced, reduce the impact that sample is produced by laser；Additionally, metal nanoparticle is short for action time with testing sample in method of the present utility model, greatly reduce the metal nanoparticle toxic damages to biological sample, it is thus possible to farthest reaction living cells actual biological state.
Have again, lossless testing sample noncontact being suspended of near-infrared laser is fixed in liquid, again metal nanoparticle is enriched to the surface of testing sample simultaneously, makes testing sample contactless with the pool wall of sample cell, effectively reduce bottom the interference to Raman signal of the autofluorescence of slide；Simultaneously, it is to avoid mechanical injuries to testing sample.
The more important thing is, whole detection process is all rapidly completed in liquid environment, the most lossless to biological sample (such as living cells), cultivate further after being conducive to Raman spectrum detection, amplification etc. is studied, thus the optical tweezer that the testing sample after raman spectroscopy measurement can be formed with this utility model easily carries out sorting, extracting and eluting, realize Raman spectrum identification and the follow-up study of sorting function, beneficially biological sample of single living cell.
Accompanying drawing explanation
Fig. 1 is that in liquid of the present utility model, induced with laser strengthens the detection of Raman spectrum and the schematic diagram of sorting unit；
Fig. 2 is that the induced with laser obtained according to an embodiment of the detection method of induced with laser enhancing Raman spectrum in liquid of the present utility model strengthens Raman spectrogram.
Detailed description of the invention
Below in conjunction with the accompanying drawings, by specific embodiment, this utility model is expanded on further.
As it is shown in figure 1, the detection of induced with laser enhancing Raman spectrum includes with sorting unit in the liquid of the present embodiment: induction electro-optical device 1, excitation-light unit 2, light combination mirror the 3, first dichroic mirror 4, object lens 5, sample cell 6, metal nanoparticle, condenser lens, Raman spectrometer 7, illumination light device 8 and computer 9；Wherein, holding, in transparent sample cell 6, the testing sample being dissolved in liquid, metal nanoparticle is injected in the liquid of sample cell；Induction electro-optical device 1 sends near-infrared laser as induction light, first through light combination mirror 3 after the first reflecting mirror M1 reflection, it is reflected into object lens 5 by the first dichroic mirror 4, by object lens 5 high order focusing, focusing to from the bottom of sample cell in sample cell 6, focus is positioned on the testing sample being suspended in sample cell 6, forms three-dimensional ligh trap, suspend fixing testing sample, and enriched in metals nano-particle is in the surface of testing sample；Excitation-light unit 2 sends the laser of visible light wave range, as exciting light, first after the second reflecting mirror M2 reflection after light combination mirror 3 with induction combiner, it is reflected into object lens by the first dichroic mirror 4, by object lens 5 high order focusing, focus is similarly positioned on the testing sample in sample cell, the nano-metal particle being enriched in testing sample surface is excited to produce local surface plasma resonance LSPR effect, excite testing sample to produce Raman signal simultaneously, Raman signal interacts with local surface plasma resonance LSPR effect, forms induced with laser and strengthens Raman signal；Induced with laser strengthens Raman signal and is collected by object lens 5, through the first dichroic mirror 4 transmission, second dichroic mirror 83 reflects, reflect through the 3rd reflecting mirror M3, after exciting light wave filter 71 filters Rayleigh scattering light, line focus lens 72 focus on, and enter Raman spectrometer 7, obtain induced with laser and strengthen Raman spectrum；Raman spectrometer 7 is connected to computer 9 through data wire, and what Raman scattering was surveyed is the vibration signal of molecular link, and the Raman spectrum recorded by analysis can deduce composition and the relative amount of testing sample further.Light combination mirror 3 uses dichroic mirror.Exciting light wave filter 71 uses notch filter.
In the present embodiment, sample cell 6 uses thickness to be about punching in the middle of the organic plastics of 5mm, and quartz wave-plate 62 is pasted in bottom, the Cover Glass 61 of quartz in top cover.Testing sample is pyridine, and liquid uses the silver sol that 1mL concentrates, and adds the NaCl saline solution of 10uL, 1M, and final concentration is about 10mM.
The material of metal nanoparticle uses silver-colored, and the diameter of silver nano-grain about 60nm is spherical.
Induction electro-optical device 1 includes: near infrared laser the 11, first electronic shutter 12 and coupled lens group 13；Wherein, near infrared laser 1 sends single transverse mode lasers of wavelength 1064nm, and through the first electronic shutter 12, coupled lens group 13 after expanding, the diameter of light beam, more than the diameter of the rear pupil of object lens, forms induction light.
Excitation-light unit 2 includes: solid state laser the 21, second electronic shutter 22 and beam expanding lens group 23；Wherein, solid state laser 21 sends 532nm (500mW) TEM00 mould laser, beam expanding lens group expand 23, forms exciting light, arranges the second electronic shutter 22 in beam expanding lens group.
Illumination light device 8 is arranged on above sample cell, illumination light device includes bright field illumination Halogen light 81 and condenser lens 82, bright field illumination Halogen light 81 launches bright field light, it is radiated at after being focused on by condenser lens 82 in sample cell 6, sample, after object lens 5 focus on, through the first dichroic mirror 4 and the second dichroic mirror 83 transmission, is imaged on imaging device 84, imaging device 84 is connected to monitor 85 through data wire, in real time display testing sample within sample cell.Imaging device 84 is connected to computer 9 by data wire, it is achieved the control to imaging device.Imaging device uses CCD camera.
In the liquid of the present embodiment, the detection method of induced with laser enhancing Raman spectrum includes:
1) prepared by sample:
A) prepared by metallic particles: metal nanoparticle uses silver, and silver nano-grain is present in silver sol；
B) prepared by sample: the pyridine of 99% analytical pure concentration dilutes 10 times, the NaCl solution of preparation 1M；
C) by 10uL NaCl solution, 8uL pyridine injects mix homogeneously in 1mL silver sol；
D) sample cell, covered are injected.
2) opening illumination light device, illumination light device launches bright field light, is radiated in sample cell, after object lens focus on, respectively after the first and second dichroic mirror transmissions, it is imaged on imaging device, and by image transmitting to monitor, display testing sample within sample cell in real time.
3) illumination light device is closed.
4) induction electro-optical device is opened, induction electro-optical device sends 1064nm laser as induction light, after light combination mirror, it is reflected into object lens again through the first dichroic mirror, by the object lens high order focusing of high-NA, focus is positioned on the testing sample being suspended in sample cell, 5～10 μm bottom sample cell, forming three-dimensional ligh trap, enriched in metals nano-particle is in the surface of testing sample.
5) excitation-light unit is opened, excitation-light unit sends 532nm (500mW) TEM00 mould laser, as exciting light, after light combination mirror with induction combiner, object lens are entered through the first dichroic mirror, by the object lens high order focusing of high-NA, focus is similarly positioned on the testing sample in sample cell, the nano-metal particle being enriched in testing sample surface is excited to produce local surface plasma resonance LSPR effect, excite testing sample to produce Raman signal simultaneously, Raman signal interacts with local surface plasma resonance LSPR effect, form induced with laser and strengthen Raman signal.
6) induced with laser enhancing Raman signal is collected by object lens, through the first dichroic mirror and induction light and exciting light branch, through the second dichroic mirror and illumination light branch, through exciting light wave filter, again after line focus lens focus, enter Raman spectrometer, obtain induced with laser and strengthen Raman spectrum.Raman spectrum is received by the liquid nitrogen refrigerating spectrum CCD that spectrogrph is subsidiary.
7) Raman spectrometer sends data to computer, analyzes induced with laser enhancing Raman spectrum and obtains composition and the relative amount of testing sample.
Fig. 2 is that the induced with laser of the present embodiment strengthens one group of data that Raman spectroscopy device is gathered.In Fig. 2, X represents Raman shift, unit cm-1；Y-axis represents Raman scattering light intensity, arbitrary unit.Smooth solid line represents that induced with laser strengthens Raman spectrum signal, and the band warning triangle solid line pressing close to X-axis represents spontaneous Raman spectrum signal.Spontaneous Raman group distilled water replaces nano-Ag particles, and other keep consistent with experimental group.
Under induced with laser of the present utility model strengthens Raman spectroscopy device, Pyridine Molecules enhancing Raman, compared with spontaneous Raman spectral signal, strengthens more than two orders of magnitude.
It is finally noted that, the purpose publicizing and implementing example is that help is further appreciated by this utility model, but it will be appreciated by those skilled in the art that: without departing from this utility model and appended spirit and scope by the claims, various substitutions and modifications are all possible.Therefore, this utility model should not be limited to embodiment disclosure of that, and the scope that this utility model claims defines in the range of standard with claims.
Claims (10)
1. in a liquid, induced with laser strengthens detection and the sorting unit of Raman spectrum, it is characterised in that described detection and sorting unit
Including: induction electro-optical device, excitation-light unit, light combination mirror, the first dichroic mirror, object lens, sample cell, metal nanoparticle,
Condenser lens, exciting light wave filter, Raman spectrometer and computer；Wherein, testing sample uniformly mixes with metal nanoparticle
Close, be dissolved in the liquid being contained in transparent sample cell；Described induction electro-optical device send near-infrared laser as induction light,
Through light combination mirror, then entering object lens through the first dichroic mirror, by the object lens high order focusing of high-NA, focus is positioned at and is suspended in
On testing sample in sample cell, forming three-dimensional ligh trap in a liquid, suspend fixing testing sample, and enriched in metals nanometer
Grain is in the surface of testing sample；Described excitation-light unit sends the laser of visible light wave range, as exciting light, through light combination mirror with
After induction combiner, entering object lens through the first dichroic mirror, by the object lens high order focusing of high-NA, focus is similarly positioned in
On testing sample in sample cell, form induced with laser and strengthen Raman signal；Described induced with laser strengthens Raman signal by object lens
After focusing, through the first dichroic mirror and exciting light and induction light branch, through exciting light wave filter, then line focus lens focus
After, enter Raman spectrometer, obtain induced with laser and strengthen Raman spectrum；Described Raman spectrometer is connected to calculate through data wire
Machine.
2. detection as claimed in claim 1 and sorting unit, it is characterised in that described sample cell uses non-blooming transparent material.
3. detection as claimed in claim 1 and sorting unit, it is characterised in that if testing sample be cell, albumen, microorganism and
One or more in chemical molecular, described liquid uses the one in buffer, culture fluid and aqueous solution；If testing sample
For the solid particulate matter in air, described liquid is aqueous solution or organic solvent.
4. detection as claimed in claim 1 and sorting unit, it is characterised in that the material of described metal nanoparticle uses coin gold
Belong to.
5. detection as claimed in claim 4 and sorting unit, it is characterised in that the material of described metal nanoparticle uses gold, silver
One in platinum and copper.
6. detection as claimed in claim 1 and sorting unit, it is characterised in that the incident wavelength of described laser is more than metal nano
The diameter 10 times of grain, the diameter of described metal nanoparticle is less than 100nm.
7. detection as claimed in claim 1 and sorting unit, it is characterised in that described induction electro-optical device includes: near infrared laser,
First electronic shutter and coupled lens group；Wherein, described near infrared laser sends near-infrared laser, through the first electronic shutter,
After being expanded by coupled lens group, the diameter of light beam, more than the diameter of the rear pupil of object lens, forms induction light.
8. detection as claimed in claim 1 and sorting unit, it is characterised in that described excitation-light unit includes: solid state laser,
Second electronic shutter and beam expanding lens group；Wherein, described solid state laser sends the laser of visible light wave range, beam expanding lens group expand
Bundle, forms exciting light, arranges the second electronic shutter in beam expanding lens group.
9. detection as claimed in claim 1 and sorting unit, it is characterised in that also include illumination light device, the second dichroic mirror,
Imaging device and monitor, arrange illumination light device, and the outer and thing at sample cell in the outer side relative with object lens of sample cell
Mirror homonymy arranges imaging device；Described illumination light device launches bright field light, is radiated in sample cell, after object lens focus on,
Through the first dichroic mirror and induction light branch, then strengthen Raman signal branch through the second dichroic mirror with induced with laser, be imaged on
On imaging device, described imaging device is connected to monitor through data wire.
10. detection as claimed in claim 1 and sorting unit, it is characterised in that also include capillary tube, be fixed in sample cell,
The internal diameter of described capillary tube is more than the size of selected sample, and one end of capillary tube is placed in sample cell, the other end and micro-note
Emitter is connected.
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| CN105758838A (en) * | 2016-04-11 | 2016-07-13 | 北京大学 | Method and device for detecting and selecting laser-induce enhanced Raman spectrum in liquid |
| CN107907526A (en) * | 2017-10-13 | 2018-04-13 | 中国科学院上海技术物理研究所 | A kind of adaptive Raman fluorescence imaging combined system of survey of deep space microcell |
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| CN109406491A (en) * | 2018-11-27 | 2019-03-01 | 北京工业大学 | Body towards liquid sensing detection enhances Raman spectroscopy test device |
| CN110006873A (en) * | 2019-04-08 | 2019-07-12 | 重庆市环卫集团有限公司 | Environmental pollutant detection method based on three-dimensional micro-nano structure enhancing Raman spectrum |
| CN112585448A (en) * | 2018-06-19 | 2021-03-30 | 帝国科学、技术与医学学院 | Single particle automated raman trapping analysis |
| CN113670799A (en) * | 2021-08-18 | 2021-11-19 | 长春长光辰英生物科学仪器有限公司 | Single-cell two-photon Raman recognition and accurate sorting instrument and sorting method thereof |
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| CN117405649A (en) * | 2023-12-12 | 2024-01-16 | 中国科学院长春光学精密机械与物理研究所 | Cell Raman flow spectrum imaging analysis system and analysis method |
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| CN105758838B (en) * | 2016-04-11 | 2018-08-10 | 北京大学 | The detection of induced with laser enhancing Raman spectrum and method for separating and its device in liquid |
| CN105758838A (en) * | 2016-04-11 | 2016-07-13 | 北京大学 | Method and device for detecting and selecting laser-induce enhanced Raman spectrum in liquid |
| CN107907526B (en) * | 2017-10-13 | 2023-09-12 | 中国科学院上海技术物理研究所 | Deep space detection micro-region self-adaptive Raman fluorescence imaging combined system |
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| CN112585448A (en) * | 2018-06-19 | 2021-03-30 | 帝国科学、技术与医学学院 | Single particle automated raman trapping analysis |
| CN109182444A (en) * | 2018-09-12 | 2019-01-11 | 珠海彩晶光谱科技有限公司 | A kind of method and apparatus of detection Pathogen category distribution and drug resistance |
| CN109243660A (en) * | 2018-09-19 | 2019-01-18 | 深圳大学 | A kind of SPP light forceps device based on chiral dependence lens excitation |
| CN109243660B (en) * | 2018-09-19 | 2024-04-30 | 深圳大学 | SPP optical tweezers device based on chiral dependence lens excitation |
| CN109406491A (en) * | 2018-11-27 | 2019-03-01 | 北京工业大学 | Body towards liquid sensing detection enhances Raman spectroscopy test device |
| CN110006873A (en) * | 2019-04-08 | 2019-07-12 | 重庆市环卫集团有限公司 | Environmental pollutant detection method based on three-dimensional micro-nano structure enhancing Raman spectrum |
| CN113670799A (en) * | 2021-08-18 | 2021-11-19 | 长春长光辰英生物科学仪器有限公司 | Single-cell two-photon Raman recognition and accurate sorting instrument and sorting method thereof |
| CN113670799B (en) * | 2021-08-18 | 2023-12-26 | 长春长光辰英生物科学仪器有限公司 | Single-cell two-photon Raman recognition and accurate sorting instrument and sorting method thereof |
| CN114577681B (en) * | 2022-05-07 | 2022-09-09 | 之江实验室 | Aerosol weak Raman spectrum signal detection device and application method thereof |
| CN114577681A (en) * | 2022-05-07 | 2022-06-03 | 之江实验室 | Aerosol weak Raman spectrum signal detection device and application method thereof |
| CN117405649A (en) * | 2023-12-12 | 2024-01-16 | 中国科学院长春光学精密机械与物理研究所 | Cell Raman flow spectrum imaging analysis system and analysis method |
| CN117405649B (en) * | 2023-12-12 | 2024-03-22 | 中国科学院长春光学精密机械与物理研究所 | Cell Raman flow spectrum imaging analysis system and analysis method |
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