CN113663365A - Bubble trap mechanism and chromatography system - Google Patents
Bubble trap mechanism and chromatography system Download PDFInfo
- Publication number
- CN113663365A CN113663365A CN202010406327.4A CN202010406327A CN113663365A CN 113663365 A CN113663365 A CN 113663365A CN 202010406327 A CN202010406327 A CN 202010406327A CN 113663365 A CN113663365 A CN 113663365A
- Authority
- CN
- China
- Prior art keywords
- trap
- pipe
- bottle
- chromatography
- bubble trap
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 230000007246 mechanism Effects 0.000 title claims abstract description 82
- 238000004587 chromatography analysis Methods 0.000 title claims abstract description 79
- 239000007788 liquid Substances 0.000 claims abstract description 150
- 239000011521 glass Substances 0.000 claims description 7
- 229910052751 metal Inorganic materials 0.000 claims description 7
- 239000002184 metal Substances 0.000 claims description 7
- 229920001296 polysiloxane Polymers 0.000 claims description 4
- 238000004140 cleaning Methods 0.000 abstract description 15
- 238000004659 sterilization and disinfection Methods 0.000 abstract description 13
- 238000005406 washing Methods 0.000 abstract description 4
- 238000004891 communication Methods 0.000 description 9
- 230000008878 coupling Effects 0.000 description 6
- 238000010168 coupling process Methods 0.000 description 6
- 238000005859 coupling reaction Methods 0.000 description 6
- 230000000249 desinfective effect Effects 0.000 description 6
- 238000000034 method Methods 0.000 description 6
- 238000005086 pumping Methods 0.000 description 6
- 230000001954 sterilising effect Effects 0.000 description 6
- 101100139835 Homo sapiens RAC1 gene Proteins 0.000 description 5
- 102100022122 Ras-related C3 botulinum toxin substrate 1 Human genes 0.000 description 5
- 239000003513 alkali Substances 0.000 description 4
- CDBYLPFSWZWCQE-UHFFFAOYSA-L Sodium Carbonate Chemical compound [Na+].[Na+].[O-]C([O-])=O CDBYLPFSWZWCQE-UHFFFAOYSA-L 0.000 description 3
- 239000000463 material Substances 0.000 description 2
- 229910052782 aluminium Inorganic materials 0.000 description 1
- XAGFODPZIPBFFR-UHFFFAOYSA-N aluminium Chemical compound [Al] XAGFODPZIPBFFR-UHFFFAOYSA-N 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000010828 elution Methods 0.000 description 1
- 238000002386 leaching Methods 0.000 description 1
- 230000013011 mating Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 229910000029 sodium carbonate Inorganic materials 0.000 description 1
Images
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
- B01D15/08—Selective adsorption, e.g. chromatography
- B01D15/10—Selective adsorption, e.g. chromatography characterised by constructional or operational features
- B01D15/14—Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to the introduction of the feed to the apparatus
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
- B01D15/08—Selective adsorption, e.g. chromatography
- B01D15/10—Selective adsorption, e.g. chromatography characterised by constructional or operational features
- B01D15/20—Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to the conditioning of the sorbent material
- B01D15/203—Equilibration or regeneration
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
- B01D15/08—Selective adsorption, e.g. chromatography
- B01D15/10—Selective adsorption, e.g. chromatography characterised by constructional or operational features
- B01D15/22—Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to the construction of the column
Landscapes
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention discloses a bubble trap mechanism and a chromatography system. The bubble trap mechanism comprises a plurality of bubble trap units, each bubble trap unit comprises a trap bottle, a liquid inlet pipe, a liquid outlet pipe, a vent pipe and a control valve, the liquid inlet pipes, the liquid outlet pipes and the vent pipes are all communicated with the trap bottles through the tops of the trap bottles, the control valves are arranged on the liquid inlet pipes, the liquid outlet pipes and the vent pipes, the end portions, located in the trap bottles, of the liquid outlet pipes extend to the bottoms of the trap bottles, the end portions, located in the trap bottles, of the vent pipes are flush with the tops of the trap bottles, the outward end portions of the liquid inlet pipes are used for being communicated with liquid inlets of the chromatography liquid inlet mechanism, and the liquid outlet pipes are used for being communicated with the chromatography mechanism. The chromatography system comprises a bubble trap mechanism. The bubble trap mechanism solves the problem of pollution risk caused by frequent emptying, washing, incomplete cleaning and disinfection due to sharing of multiple chromatography solutions in the prior art, simplifies operation, saves time and reduces consumption of various chromatography solutions in chromatography work.
Description
Technical Field
The invention relates to the technical field of biology, in particular to a bubble trap mechanism and a chromatography system.
Background
The chromatographic system is provided with a cylindrical bubble trap device before being connected with a valve of a chromatographic column, so that the condition that bubbles in a solution enter the chromatographic column to cause the performance reduction of the chromatographic column and finally influence the chromatographic effect can be prevented. The chromatographic process generally uses multiple chromatographic solutions, such as a balance solution, a leaching solution, an elution solution, a cleaning solution, a sample loading material liquid and the like, a chromatographic system only has one bubble trap device, the sample loading material liquid directly enters a chromatographic column without passing through the bubble trap device in the prior art, other chromatographic solutions enter the bubble trap device, the original chromatographic solution in the bubble trap device needs to be discharged every time when one chromatographic solution is replaced, and the chromatographic solution is repeatedly washed for multiple times to completely eliminate the influence of the original solution, so that the steps are complicated, and the operation time and the consumption of various chromatographic solutions are increased. Meanwhile, the bubble trap device can only be cleaned and disinfected by alkali liquor and the like, when the bubble trap device is filled with the bubbles and discharged from the upper opening, the top of the bubble trap device cannot be cleaned or disinfected, and solution in an area with the bubbles cannot be contacted, so that the hidden danger of pollution is left due to incomplete cleaning and disinfection.
Disclosure of Invention
Based on this, it is necessary to provide a bubble trap mechanism and a chromatography system including the bubble trap mechanism, which can simplify the operation, save the time, and reduce the consumption of various chromatography solutions, in view of the problem of the prior art that the multiple chromatography solutions are shared to cause the risk of contamination due to frequent emptying and washing, and incomplete cleaning and disinfection.
The utility model provides a bubble trap mechanism, includes a plurality of bubble trap units, bubble trap unit includes trap bottle, feed liquor pipe, drain pipe, breather pipe and control valve, the feed liquor pipe the drain pipe and the breather pipe all passes through the top of trap bottle with trap bottle intercommunication, the feed liquor pipe the drain pipe and all be provided with on the breather pipe the control valve, the drain pipe is located tip in the trap bottle extends to the bottle bottom of trap bottle, the breather pipe is located tip in the trap bottle with the bottle top of trap bottle flushes, feed liquor pipe tip outwards be used for with each inlet intercommunication of chromatography feed liquor mechanism, the drain pipe is used for with chromatography mechanism intercommunication.
In one embodiment, the control valve is a wafer chuck.
In one embodiment, the end of the liquid inlet pipe extends to the middle position of the trap bottle.
In one embodiment, the end part of the liquid outlet pipe in the trap bottle is in a bevel mouth shape, and the end part of the liquid outlet pipe in the trap bottle extends to the bottle bottom of the trap bottle to abut against.
In one embodiment, the bubble trap unit further comprises quick joints, and the quick joints are arranged on the outward end of the liquid inlet pipe, the outward end of the liquid outlet pipe and the outward end of the air pipe.
In one embodiment, the quick connect coupling is a snap-fit coupling.
In one embodiment, an opening is formed in the top of the trap bottle, a three-way cover is arranged at the opening, and the liquid inlet pipe, the liquid outlet pipe and the air pipe are respectively communicated with the trap bottle through the three-way cover.
In one embodiment, the liquid inlet pipe, the liquid outlet pipe and/or the air vent pipe are silicone tubes, plastic tubes, glass tubes or metal tubes.
In one embodiment, the bubble trap unit further comprises a communicating pipe and a chuck, wherein one end of the communicating pipe is communicated with the outward end of the liquid inlet pipe, and the other end of the communicating pipe is used for being communicated with the chromatography liquid inlet mechanism through the chuck.
In one embodiment, the trap bottle is a PP bottle, a glass bottle or a metal bottle.
In one embodiment, the bubble trap mechanism further comprises a multi-way pipe, each liquid inlet pipe is communicated with one port of the multi-way pipe, and one port of the multi-way pipe is used for being communicated with the chromatography liquid inlet mechanism through a chuck.
The invention also provides a chromatography system.
A chromatography system comprising said bubble trap mechanism.
The bubble trap mechanism solves the problem of pollution risk caused by frequent emptying, washing, incomplete cleaning and disinfection due to sharing of multiple chromatography solutions in the prior art, simplifies operation, saves time and reduces consumption of various chromatography solutions in chromatography work.
When the bubble trap mechanism is used, before the bubble trap mechanism is externally connected with a chromatographic system, a trap bottle and various pipelines such as a liquid inlet pipe, a liquid outlet pipe, a vent pipe and the like can be disassembled from sodium carbonate and cleaned, and the bubble trap mechanism is subjected to damp-heat sterilization before being used, so that the risk of incomplete cleaning and sterilization of the traditional bubble trap is avoided.
When the liquid inlet of the chromatography system is insufficient, the liquid inlet of the chromatography system can be simultaneously communicated with a plurality of bubble trap units by arranging the multi-way pipe, when one chromatography solution is used, the multi-way pipe is clamped with a pipeline connected with other chromatography solutions by a control valve, and then normal chromatography operation can be started.
According to the bubble trap mechanism, the end part of the liquid outlet pipe, which is positioned in the trap bottle, is in an inclined opening shape, and the end part of the liquid outlet pipe, which is positioned in the trap bottle, extends to the bottom of the trap bottle to be abutted, so that the trap bottle can completely discharge liquid, and the residue of the liquid in the trap bottle is reduced.
The use method of the bubble trap mechanism comprises the following steps: and (4) carrying out damp-heat sterilization on the trap bottle, the liquid inlet pipe, the liquid outlet pipe, the communicating pipe and the vent pipe of the bubble trap mechanism. Then, the communicating pipe is connected with the chromatography system through a TC25 chuck, cleaning and disinfecting solution bags/sample barrels are connected with the communicating pipe through quick-connection aseptic operation, for example, alkali liquor is connected with the communicating pipe to clean and disinfect each pipeline and the chromatography system, then the liquid outlet pipe of the trap bottle is connected with the chromatography system through the quick-connection aseptic operation, then the corresponding chromatography solution/sample bag/sample barrel is connected with the communicating pipe on the trap bottle through the quick-connection aseptic operation, and each chromatography solution/sample feeding liquid corresponds to one bubble trap mechanism. During operation, firstly pumping the chromatographic solution/sample loading liquid into the trap bottle, simultaneously starting the chromatographic system to pump the chromatographic solution/sample loading liquid out of the trap bottle, removing air in each pipeline, finally maintaining the liquid level height in the trap bottle to be 2/3 about the height of the trap bottle, stopping pumping the liquid into the trap bottle, simultaneously stopping the work of a pump of the chromatographic system, clamping a control valve corresponding to a vent pipe of the trap bottle, simultaneously keeping the liquid inlet pipe and the liquid outlet pipe of the trap bottle to be smooth, and starting the chromatographic mechanism to work.
Drawings
FIG. 1 is a schematic view of a bubble trap mechanism according to embodiment 1 of the present invention;
fig. 2 is a schematic view of a bubble trap mechanism according to embodiment 2 of the present invention.
Description of the reference numerals
10. A bubble trap mechanism; 100. a bubble trap unit; 101. a trap bottle; 102. a liquid inlet pipe; 103. a liquid outlet pipe; 104. a breather pipe; 105. a control valve; 106. a quick-connect joint; 107. a communicating pipe; 108. a chuck; 200. a multi-pass tube; 20. a chromatography system.
Detailed Description
To facilitate an understanding of the invention, the invention will now be described more fully with reference to the accompanying drawings. Preferred embodiments of the present invention are shown in the drawings. This invention may, however, be embodied in many different forms and should not be construed as limited to the embodiments set forth herein. Rather, these embodiments are provided so that this disclosure will be thorough and complete.
In the description of the present invention, it should be understood that the terms used in the present invention are used in the description of the present invention, and it should be understood that the directions or positional relationships indicated by the terms "center", "upper", "lower", "bottom", "inner", "outer", etc. in the present invention are based on the directions or positional relationships shown in the drawings, and are only for convenience of description and simplicity of description, but do not indicate or imply that the device or element referred to must have a specific orientation, be constructed in a specific orientation, and be operated, and thus, should not be construed as limiting the present invention.
It should be understood that the terms "first", "second", etc. are used herein to describe various information, but the information should not be limited to these terms, which are only used to distinguish one type of information from another. For example, "first" information may also be referred to as "second" information, and similarly, "second" information may also be referred to as "first" information, without departing from the scope of the present invention.
In the description of the present invention, it is to be noted that, unless otherwise explicitly specified or limited, the terms "connected" and "connected" are to be interpreted broadly, e.g., as being fixed or detachable or integrally connected; can be mechanically or electrically connected; they may be directly connected or indirectly connected through intervening elements, or they may be in communication within two elements, i.e., when an element is referred to as being "secured to" another element, it may be directly on the other element or intervening elements may also be present. When an element is referred to as being "connected" to another element, it can be directly connected to the other element or intervening elements may also be present. The specific meanings of the above terms in the present invention can be understood in specific cases to those skilled in the art.
Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. The terminology used in the description of the invention herein is for the purpose of describing particular embodiments only and is not intended to be limiting of the invention. As used herein, the term "and/or" includes any and all combinations of one or more of the associated listed items. The term "plurality" as used herein means "at least two".
Referring to fig. 1, an embodiment of the present invention provides a bubble trap mechanism 10.
The bubble trap mechanism 10 includes a plurality of bubble trap cells 100. Bubble trap unit 100 includes a trap bottle 101, an inlet pipe 102, an outlet pipe 103, an air pipe 104, and a control valve 105.
Preferably, the control valve 105 is a wafer chuck.
In one particular example, the end of the inlet pipe 102 extends to a middle position of the trap bottle 101.
In a specific example, the end of drain pipe 103 in trap bottle 101 is in a bevel shape, and the end of drain pipe 103 in trap bottle 101 extends to the bottom of trap bottle 101 to abut against. According to the bubble trap mechanism 10, the end part of the liquid outlet pipe 103, which is positioned in the trap bottle 101, is in an inclined opening shape, and the end part of the liquid outlet pipe 103, which is positioned in the trap bottle 101, extends to the bottom of the trap bottle 101 to be abutted, so that the trap bottle 101 can completely discharge liquid, and the residue of the liquid in the trap bottle 101 is reduced.
In one specific example, the bubble trap unit 100 further includes a quick-connect fitting 106, and the quick-connect fitting 106 is disposed on an outward end of the liquid inlet pipe 102, an outward end of the liquid outlet pipe 103, and an outward end of the gas vent pipe 104.
In one particular example, the quick connect fitting 106 is a snap-fit fitting. The snap-fit joint 106 enables quick mating and unmating.
In one specific example, the top of the trap bottle 101 is provided with an opening and the opening is provided with a three-way cover, and the liquid inlet pipe 102, the liquid outlet pipe 103 and the air vent pipe 104 are respectively communicated with the trap bottle 101 through the three-way cover.
In one particular example, inlet tube 102, and/or outlet tube 103, and/or vent tube 104 is a silicone tube, a plastic tube, a glass tube, or a metal tube.
In a specific example, the bubble trap unit 100 further includes a communication pipe 107 and a cartridge 108, one end of the communication pipe 107 is communicated with the outward end of the liquid inlet pipe 102, and the other end is used for being communicated with the chromatography liquid inlet mechanism through the cartridge 108.
In a specific example, the trap bottle 101 is a PP bottle, a glass bottle, or a metal bottle. The volume of the trap bottle 101 is 1L-2L. For example, the volume of the trap bottle 101 is 1L, 1.5L, 2L, or other non-integer value.
In one particular example, the bubble trap mechanism 10 further includes a multi-channel tube 200. Each liquid inlet pipe 102 is respectively communicated with one port of the multi-way pipe 200, and one port of the multi-way pipe 200 is communicated with a chromatography liquid inlet mechanism through a chuck. When the liquid inlet of the chromatography system 20 is insufficient, the present invention can realize that the liquid inlet of the chromatography system 20 is simultaneously communicated with a plurality of bubble trap units 100 by arranging the multi-way tube 200, and when one of the chromatography solutions is used, the tube connecting the multi-way tube 200 with the other chromatography solution is clamped by the control valve 105, and then normal chromatography operation can be started.
The bubble trap mechanism 10 solves the problem of pollution risk caused by frequent emptying, washing, incomplete cleaning and disinfection due to sharing of multiple chromatography solutions in the prior art, simplifies operation, saves time and reduces consumption of various chromatography solutions in chromatography work. When the bubble trap mechanism 10 is used, before the external chromatography system 20 is connected, the trap bottle 101 and various pipelines such as the liquid inlet pipe 102, the liquid outlet pipe 103, the vent pipe 104 and the like can be disassembled from soda and cleaned, and the wet heat sterilization is carried out before the bubble trap mechanism is used, so that the risk of incomplete cleaning and disinfection of the traditional bubble trap is avoided.
An embodiment of the invention also provides a chromatography system.
The chromatography system comprises a chromatography mechanism 20 and the bubble trap mechanism 10. Referring to fig. 1, the chromatographic mechanism 20 is shown in cooperation with a bubble trap mechanism 10 comprising two bubble trap units 100.
Referring to fig. 2, the chromatographic mechanism 20 is shown in cooperation with a bubble trap mechanism 10 comprising three bubble trap units 100.
Example 1
Referring to fig. 1, the present embodiment provides a bubble trap mechanism 10.
The bubble trap mechanism 10 includes a plurality of bubble trap cells 100. Bubble trap unit 100 includes a trap bottle 101, an inlet pipe 102, an outlet pipe 103, an air pipe 104, and a control valve 105. The trap bottle 101 is a PP bottle. The volume of the trap bottle 101 is 2L.
The bubble trap unit 100 further includes a connection pipe 107 and a chuck 108. One end of the connection pipe 107 is connected to the outward end of the liquid inlet pipe 102, and the other end is connected to the chromatographic liquid inlet mechanism via the cartridge 108. The cartridge 108 is model TC25 cartridge 108.
The connection pipe 107 connected with each liquid inlet pipe 102 is respectively used for being communicated with each liquid inlet of the chromatography liquid inlet mechanism. As shown in FIG. 1, a plurality of bubble trap units 100 are arranged in parallel and are respectively communicated with each liquid inlet of the chromatography liquid inlet mechanism through respective communication pipes 107. In the present embodiment, the communication pipes 107 are arranged in parallel.
The end of the inlet pipe 102 extends to the middle of the trap bottle 101.
The end of the liquid outlet pipe 103 in the trap bottle 101 is in a bevel shape, and the end of the liquid outlet pipe 103 in the trap bottle 101 extends to the bottom of the trap bottle 101 to be abutted. In the bubble trap mechanism 10 of this embodiment, the end of the liquid outlet pipe 103 in the trap bottle 101 is in an oblique shape, and the end of the liquid outlet pipe 103 in the trap bottle 101 extends to the bottom of the trap bottle 101 to abut against the bottom, so that the trap bottle 101 can completely discharge liquid, and the residue of the liquid in the trap bottle 101 is reduced.
The quick connect coupling 106 is a snap-fit coupling.
An opening is formed in the top of the trap bottle 101, a three-way cover is arranged at the opening, and the liquid inlet pipe 102, the liquid outlet pipe 103 and the vent pipe 104 are respectively communicated with the trap bottle 101 through the three-way cover. Specifically, the top of the trap bottle 101 protrudes with a bottle neck, and the three-way cap is detachably connected with the bottle neck, for example, the three-way cap has an internal thread, the bottle neck has an external thread, and the three-way cap is connected with the bottle neck through a thread.
Example 2
Referring to fig. 2, the present embodiment provides a bubble trap mechanism 10.
The bubble trap mechanism 10 includes a plurality of bubble trap cells 100. The bubble trap unit 100 comprises a trap bottle 101, an inlet pipe 102, an outlet pipe 103, an outlet pipe 104, a multi-way pipe 200, and a control valve 105. The trap bottle 101 is a glass bottle. The volume of the trap bottle 101 is 2L.
The bubble trap unit 100 further includes a connection pipe 107 and a chuck 108. One end of the connection pipe 107 is connected to the outward end of the liquid inlet pipe 102, and the other end is connected to the chromatographic liquid inlet mechanism via the cartridge 108. The cartridge 108 is model TC25 cartridge 108.
Referring to fig. 2, the connection pipe 107 connected to each liquid inlet pipe 102 is communicated through a multi-way pipe 200, and each port of the multi-way pipe 200 is respectively used for being communicated with each liquid inlet of the chromatography liquid inlet mechanism. In the present embodiment, the communication pipes 107 communicate with each other through the multi-way pipe 200.
When the liquid inlet of the chromatography system 20 is insufficient, the liquid inlet of the chromatography system 20 can be simultaneously communicated with a plurality of bubble trap units 100 by arranging the multi-way pipe 200, the multi-way pipe 200 is clamped by the control valve 105 when one of the chromatography solutions is used, and then normal chromatography operation can be started.
The end of the inlet pipe 102 extends to the middle of the trap bottle 101.
The end of the liquid outlet pipe 103 in the trap bottle 101 is in a bevel shape, and the end of the liquid outlet pipe 103 in the trap bottle 101 extends to the bottom of the trap bottle 101 to be abutted. According to the bubble trap mechanism 10, the end part of the liquid outlet pipe 103, which is positioned in the trap bottle 101, is in an inclined opening shape, and the end part of the liquid outlet pipe 103, which is positioned in the trap bottle 101, extends to the bottom of the trap bottle 101 to be abutted, so that the trap bottle 101 can completely discharge liquid, and the residue of the liquid in the trap bottle 101 is reduced.
The bubble trap unit 100 also includes a quick connect fitting 106. Quick-connect fittings 106 are provided on the outward end of liquid inlet pipe 102, the outward end of liquid outlet pipe 103, and the outward end of air vent pipe 104. The quick connect coupling 106 is a snap-fit coupling.
An opening is formed in the top of the trap bottle 101, a three-way cover is arranged at the opening, and the liquid inlet pipe 102, the liquid outlet pipe 103 and the vent pipe 104 are respectively communicated with the trap bottle 101 through the three-way cover. Specifically, the top of the trap bottle 101 protrudes with a bottle neck, and the three-way cap is detachably connected with the bottle neck, for example, the three-way cap has an internal thread, the bottle neck has an external thread, and the three-way cap is connected with the bottle neck through a thread.
Example 3
The present embodiment provides a method of using the bubble trap mechanism 10.
A method of using a bubble trap mechanism 10, comprising the steps of:
the trap bottle 101, the liquid inlet pipe 102, the liquid outlet pipe 103, the communicating pipe 107 and the air vent pipe 104 of the bubble trap mechanism 10 are subjected to moist heat sterilization. Then, the connection pipe 107 is connected with the chromatography system 20 through the TC25 cartridge 108, the cleaning and disinfecting solution bag/sample barrel is connected with the connection pipe 107 through the quick-connect aseptic operation, for example, the cleaning and disinfecting solution bag/sample barrel may be filled with alkali solution, cleaning and disinfecting of each pipeline and the chromatography system 20 are performed, then the liquid outlet pipe 103 of the trap bottle 101 is connected with the chromatography column of the chromatography system 20 through the quick-connect aseptic operation, and then the corresponding chromatography solution/sample bag/sample barrel is connected with the connection pipe 107 and the liquid inlet pipe 102 on the trap bottle 101 through the quick-connect aseptic operation, and each chromatography solution/sample loading liquid corresponds to one bubble trap mechanism 10.
During operation, firstly, a chromatography solution/sample liquid is pumped into the trap bottle 101, meanwhile, the chromatography system 20 is started to pump the chromatography solution/sample liquid out of the trap bottle 101, air in each pipeline is exhausted, finally, 2/3 that the liquid level height in the trap bottle 101 is about the height of the trap bottle 101 is maintained, the liquid is stopped being pumped into the trap bottle 101, meanwhile, the work of the pump of the chromatography system 20 is stopped, the control valve 105 corresponding to the vent pipe 104 of the trap bottle 101 is clamped, and meanwhile, the liquid inlet pipe 102 and the liquid outlet pipe 103 of the trap bottle 101 are kept smooth. The chromatographic mechanism 20 is put into operation.
Example 4
The present embodiment provides a method of using the bubble trap mechanism 10.
A method of using a bubble trap mechanism 10, comprising the steps of:
by adopting the multi-way tube 200 structure of embodiment 2, the trap bottle 101, the liquid inlet tube 102, the liquid outlet tube 103, the communicating tube 107, and the air vent tube 104 of the bubble trap mechanism 10 are subjected to moist heat sterilization. Then, the connection pipe 107 is connected with the chromatography system 20 through the TC25 chuck 108, the cleaning and disinfecting solution bag/sample bucket is connected with the multi-way pipe 200 through a quick-connection aseptic operation, for example, an alkali solution can be filled in the cleaning and disinfecting solution bag/sample bucket to clean and disinfect each pipeline and the chromatography system 20, after cleaning and disinfection, the liquid outlet pipe 103 on each trap bottle 101 is connected with the multi-way pipe 200 through the quick-connection aseptic operation, one port of the multi-way pipe 200 is communicated with a chromatography column of the chromatography mechanism 20, and then the corresponding chromatography solution/sample bag/sample bucket is connected with the liquid inlet pipe 102 on the trap bottle 101 through the quick-connection aseptic operation, and each chromatography solution/sample liquid corresponds to one trap bottle 101.
In operation, when one of the chromatographic solutions is used, the inlet line 102 connecting the multi-channel 200 to the other chromatographic solution is opened for chromatography and clamped by the control valve 105. Pumping chromatographic solution/sample liquid into a trap bottle 101, starting a chromatographic system 20 to pump the chromatographic solution/sample liquid out of the trap bottle 101, exhausting air in each pipeline, maintaining the liquid level height in the trap bottle 101 to be 2/3 about the height of the trap bottle 101, stopping pumping the liquid into the trap bottle 101, stopping the operation of a pump of the chromatographic system 20, clamping a control valve 105 corresponding to a vent pipe 104 of the trap bottle 101, keeping a liquid inlet pipe 102 and a liquid outlet pipe 103 of the trap bottle 101 smooth, and starting normal chromatographic operation.
After the chromatographic solution is completely loaded, the control valve 105 clamps the inlet tube 102 of the trap bottle 101 corresponding to the chromatographic solution, the control valve 105 of the inlet tube 102 of the other trap bottle 101 corresponding to the chromatographic solution to be loaded is opened, and the inlet tube 102 connecting the multi-way tube 200 and the other chromatographic solution is clamped by the control valve 105. Pumping the chromatographic solution/sample liquid into the trap bottle 101, starting the chromatographic system 20 to pump the chromatographic solution/sample liquid out of the trap bottle 101, exhausting air in each pipeline, finally maintaining the liquid level height in the trap bottle 101 to be 2/3 about the height of the trap bottle 101, stopping pumping the liquid into the trap bottle 101, stopping the operation of the pump of the chromatographic system 20, clamping the control valve 105 corresponding to the vent pipe 104 of the trap bottle 101, simultaneously keeping the liquid inlet pipe 102 and the liquid outlet pipe 103 of the trap bottle 101 smooth, and starting normal chromatographic operation. And the rest of the chromatography solutions are analogized.
The technical features of the embodiments described above may be arbitrarily combined, and for the sake of brevity, all possible combinations of the technical features in the embodiments described above are not described, but should be considered as being within the scope of the present specification as long as there is no contradiction between the combinations of the technical features.
The above-mentioned embodiments only express several embodiments of the present invention, and the description thereof is more specific and detailed, but not construed as limiting the scope of the present invention. It should be noted that, for a person skilled in the art, several variations and modifications can be made without departing from the inventive concept, which falls within the scope of the present invention. Therefore, the protection scope of the present patent shall be subject to the appended claims.
Claims (10)
1. The utility model provides a bubble trap mechanism, its characterized in that includes a plurality of bubble trap units, bubble trap unit includes trap bottle, feed liquor pipe, drain pipe, breather pipe and control valve, the feed liquor pipe the drain pipe and the breather pipe all passes through the top of trap bottle with trap bottle intercommunication, the feed liquor pipe the drain pipe and all be provided with on the breather pipe the control valve, the drain pipe is located tip in the trap bottle extends to the bottle bottom of trap bottle, the breather pipe is located tip in the trap bottle with the bottle top of trap bottle flushes, feed liquor pipe tip outwards be used for with each inlet intercommunication of chromatography feed liquor mechanism, the drain pipe be used for with chromatography mechanism intercommunication.
2. A bubble trap mechanism according to claim 1, wherein the end of the liquid inlet tube extends to a central position of the trap bottle.
3. A bubble trap mechanism according to claim 1, wherein the end of the outlet tube located within the trap bottle is beveled and extends to abut the bottom of the trap bottle.
4. A bubble trap mechanism according to claim 1, wherein the bubble trap unit further comprises quick-connect fittings provided on the outward end of the liquid inlet pipe, the outward end of the liquid outlet pipe and the outward end of the air pipe.
5. A bubble trap mechanism according to any one of claims 1-4, wherein an opening is provided at the top of the trap bottle and a three-way cover is provided at the opening, and the liquid inlet pipe, the liquid outlet pipe and the air pipe are respectively communicated with the trap bottle through the three-way cover.
6. A bubble trap mechanism according to any of claims 1-4, wherein the liquid inlet pipe, and/or the liquid outlet pipe, and/or the gas vent pipe is a silicone tube, a plastic tube, a glass tube or a metal tube.
7. The bubble trap mechanism according to any one of claims 1 to 4, wherein the bubble trap unit further comprises a communicating pipe and a chuck, one end of the communicating pipe is communicated with the outward end of the liquid inlet pipe, and the other end is used for being communicated with the chromatography liquid inlet mechanism through the chuck.
8. A bubble trap mechanism according to any of claims 1-4, wherein the trap vial is a PP vial, a glass vial or a metal vial.
9. The bubble trap mechanism according to any one of claims 1-4, further comprising a plurality of inlet pipes, wherein each inlet pipe is communicated with one port of the plurality of inlet pipes, and one port of the plurality of inlet pipes is communicated with the chromatography inlet mechanism through a chuck.
10. A chromatography system comprising a chromatography mechanism and a bubble trap mechanism according to any of claims 1-9.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010406327.4A CN113663365A (en) | 2020-05-14 | 2020-05-14 | Bubble trap mechanism and chromatography system |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010406327.4A CN113663365A (en) | 2020-05-14 | 2020-05-14 | Bubble trap mechanism and chromatography system |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN113663365A true CN113663365A (en) | 2021-11-19 |
Family
ID=78537177
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202010406327.4A Pending CN113663365A (en) | 2020-05-14 | 2020-05-14 | Bubble trap mechanism and chromatography system |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN113663365A (en) |
Citations (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1782787A (en) * | 2004-12-03 | 2006-06-07 | Lg.菲利浦Lcd株式会社 | Liquid crystal display device and method of fabricating the same |
| CN101438151A (en) * | 2006-02-09 | 2009-05-20 | 爱科来株式会社 | Liquid chromatograph |
| CN101952009A (en) * | 2007-12-20 | 2011-01-19 | 麦卡钦公司 | Rotary annular crossflow filter, degasser, and sludge thickener |
| CN103454363A (en) * | 2012-05-30 | 2013-12-18 | 爱科来株式会社 | Bubble reduction device, chromatography device, bubble reduction method, and bubble reduction program |
| CN204214822U (en) * | 2014-12-05 | 2015-03-18 | 北京佰纯润宇生物科技有限公司 | The tomographic system of the positive reflux of chromatographic column and bypass functionality is realized based on three seven logical valves |
| CN204469275U (en) * | 2013-12-18 | 2015-07-15 | 源创精科生物科技(长沙)有限公司 | The full-automatic tomographic system of a kind of list/double pump |
| CN105007958A (en) * | 2013-02-01 | 2015-10-28 | 美敦力公司 | Degassing module for a controlled compliant flow path |
| US20160001198A1 (en) * | 2013-02-26 | 2016-01-07 | The Translational Genomics Research Institute | Mobile Phase Degassing for Nano-Flow Liquid Chromatography |
| KR101721794B1 (en) * | 2016-01-15 | 2017-04-10 | 두산중공업 주식회사 | Automatic Degassing Apparatus and Method |
| CN107715498A (en) * | 2017-06-09 | 2018-02-23 | 利穗科技(苏州)有限公司 | One kind matches somebody with somebody liquid layer analysis system online |
| CN108043071A (en) * | 2017-12-12 | 2018-05-18 | 浙江凯盈新材料有限公司 | A kind of deaeration agitating device of silver paste |
| CN108970177A (en) * | 2018-06-11 | 2018-12-11 | 中国科学院过程工程研究所 | A kind of chromatographic column with bubble trap |
| CN110198771A (en) * | 2017-03-29 | 2019-09-03 | Emd密理博公司 | For handling the device of biofluid |
| CN110917658A (en) * | 2019-10-28 | 2020-03-27 | 清华大学 | Automatic strontium-90 discharging and separating device |
| CN212347797U (en) * | 2020-05-14 | 2021-01-15 | 佛山汉腾生物科技有限公司 | Bubble trap mechanism and chromatography system |
-
2020
- 2020-05-14 CN CN202010406327.4A patent/CN113663365A/en active Pending
Patent Citations (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1782787A (en) * | 2004-12-03 | 2006-06-07 | Lg.菲利浦Lcd株式会社 | Liquid crystal display device and method of fabricating the same |
| CN101438151A (en) * | 2006-02-09 | 2009-05-20 | 爱科来株式会社 | Liquid chromatograph |
| CN101952009A (en) * | 2007-12-20 | 2011-01-19 | 麦卡钦公司 | Rotary annular crossflow filter, degasser, and sludge thickener |
| CN103454363A (en) * | 2012-05-30 | 2013-12-18 | 爱科来株式会社 | Bubble reduction device, chromatography device, bubble reduction method, and bubble reduction program |
| CN105007958A (en) * | 2013-02-01 | 2015-10-28 | 美敦力公司 | Degassing module for a controlled compliant flow path |
| US20160001198A1 (en) * | 2013-02-26 | 2016-01-07 | The Translational Genomics Research Institute | Mobile Phase Degassing for Nano-Flow Liquid Chromatography |
| CN204469275U (en) * | 2013-12-18 | 2015-07-15 | 源创精科生物科技(长沙)有限公司 | The full-automatic tomographic system of a kind of list/double pump |
| CN204214822U (en) * | 2014-12-05 | 2015-03-18 | 北京佰纯润宇生物科技有限公司 | The tomographic system of the positive reflux of chromatographic column and bypass functionality is realized based on three seven logical valves |
| KR101721794B1 (en) * | 2016-01-15 | 2017-04-10 | 두산중공업 주식회사 | Automatic Degassing Apparatus and Method |
| CN110198771A (en) * | 2017-03-29 | 2019-09-03 | Emd密理博公司 | For handling the device of biofluid |
| CN107715498A (en) * | 2017-06-09 | 2018-02-23 | 利穗科技(苏州)有限公司 | One kind matches somebody with somebody liquid layer analysis system online |
| CN108043071A (en) * | 2017-12-12 | 2018-05-18 | 浙江凯盈新材料有限公司 | A kind of deaeration agitating device of silver paste |
| CN108970177A (en) * | 2018-06-11 | 2018-12-11 | 中国科学院过程工程研究所 | A kind of chromatographic column with bubble trap |
| CN110917658A (en) * | 2019-10-28 | 2020-03-27 | 清华大学 | Automatic strontium-90 discharging and separating device |
| CN212347797U (en) * | 2020-05-14 | 2021-01-15 | 佛山汉腾生物科技有限公司 | Bubble trap mechanism and chromatography system |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN106199032B (en) | A kind of sample injection method of no cross contamination | |
| US8834637B2 (en) | Biochemical analyzer and method for cleaning fluid components of the same | |
| CN102165301B (en) | Device for treating tissue | |
| CN212347797U (en) | Bubble trap mechanism and chromatography system | |
| WO2019127561A1 (en) | Waste liquid treatment device, method, and sample analyzer | |
| CN113663365A (en) | Bubble trap mechanism and chromatography system | |
| CN112871885A (en) | Cleaning device for sample adding needle, cleaning liquid path system and method thereof, and sample analyzer | |
| US20150343105A1 (en) | Cleaning/disinfecting method using endoscope cleaning/disinfecting apparatus | |
| CN210675246U (en) | Multifunctional self-cleaning liquid transfer device | |
| CN211538843U (en) | Integrated CIP cleaning system | |
| CN218872245U (en) | Fluid system and analytical instrument | |
| CN218408906U (en) | Sterile sampling butt joint device | |
| US20160158814A1 (en) | Fluid or semi-fluid dispensing system and method | |
| JP2008229557A (en) | Tank cleaning system | |
| CN105334081A (en) | Full-automatic urine sampling device and sampling method | |
| CN114806803A (en) | Cell sorting system and sorting method thereof | |
| CN214597673U (en) | Liquid filtering system with filter shell online cleaning function | |
| WO2019104580A1 (en) | Passage device for gene sequencing, operation method thereof and sequencing instrument thereof | |
| JP2000288069A (en) | Bottle for antiseptic solution | |
| CN211712712U (en) | Ozone adding system | |
| CN107208821B (en) | Blocked valve and material container blocked valve | |
| JP2016182264A (en) | Endoscope reprocessor | |
| CN110404597A (en) | A kind of Multi-function self-cleaning liquid-transfering device and its application method | |
| CN220201924U (en) | Device for continuous sampling in bioreactor culture process | |
| CN112763738B (en) | Liquid supply system |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination |