CN107715498A - One kind matches somebody with somebody liquid layer analysis system online - Google Patents
One kind matches somebody with somebody liquid layer analysis system online Download PDFInfo
- Publication number
- CN107715498A CN107715498A CN201710435890.2A CN201710435890A CN107715498A CN 107715498 A CN107715498 A CN 107715498A CN 201710435890 A CN201710435890 A CN 201710435890A CN 107715498 A CN107715498 A CN 107715498A
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- Prior art keywords
- filling opening
- solution
- chromatographic column
- somebody
- filling
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
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- 239000007788 liquid Substances 0.000 title claims abstract description 39
- 238000004458 analytical method Methods 0.000 title claims abstract description 13
- 239000000243 solution Substances 0.000 claims abstract description 67
- 230000000694 effects Effects 0.000 claims abstract description 13
- 239000011259 mixed solution Substances 0.000 claims abstract description 4
- 238000005086 pumping Methods 0.000 claims abstract description 4
- WEVYAHXRMPXWCK-UHFFFAOYSA-N Acetonitrile Chemical group CC#N WEVYAHXRMPXWCK-UHFFFAOYSA-N 0.000 claims description 9
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical group [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 claims description 9
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 6
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 5
- USFZMSVCRYTOJT-UHFFFAOYSA-N Ammonium acetate Chemical compound N.CC(O)=O USFZMSVCRYTOJT-UHFFFAOYSA-N 0.000 claims description 3
- 239000005695 Ammonium acetate Substances 0.000 claims description 3
- 229940043376 ammonium acetate Drugs 0.000 claims description 3
- 235000019257 ammonium acetate Nutrition 0.000 claims description 3
- 239000011780 sodium chloride Substances 0.000 claims description 3
- 230000004888 barrier function Effects 0.000 claims 1
- 238000006757 chemical reactions by type Methods 0.000 claims 1
- 238000007689 inspection Methods 0.000 claims 1
- 238000004587 chromatography analysis Methods 0.000 description 4
- 230000006872 improvement Effects 0.000 description 4
- 230000035611 feeding Effects 0.000 description 3
- 239000012452 mother liquor Substances 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 239000012074 organic phase Substances 0.000 description 2
- 230000005526 G1 to G0 transition Effects 0.000 description 1
- 238000005377 adsorption chromatography Methods 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 description 1
- 239000010931 gold Substances 0.000 description 1
- 229910052737 gold Inorganic materials 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 102000039446 nucleic acids Human genes 0.000 description 1
- 108020004707 nucleic acids Proteins 0.000 description 1
- 150000007523 nucleic acids Chemical class 0.000 description 1
- 150000002894 organic compounds Chemical class 0.000 description 1
- 239000005416 organic matter Substances 0.000 description 1
- 238000004810 partition chromatography Methods 0.000 description 1
- 239000012071 phase Substances 0.000 description 1
- 230000000704 physical effect Effects 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 230000004304 visual acuity Effects 0.000 description 1
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
- B01D15/08—Selective adsorption, e.g. chromatography
- B01D15/10—Selective adsorption, e.g. chromatography characterised by constructional or operational features
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
- B01D15/08—Selective adsorption, e.g. chromatography
- B01D15/10—Selective adsorption, e.g. chromatography characterised by constructional or operational features
- B01D15/12—Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to the preparation of the feed
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
- B01D15/08—Selective adsorption, e.g. chromatography
- B01D15/10—Selective adsorption, e.g. chromatography characterised by constructional or operational features
- B01D15/14—Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to the introduction of the feed to the apparatus
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
- B01D15/08—Selective adsorption, e.g. chromatography
- B01D15/10—Selective adsorption, e.g. chromatography characterised by constructional or operational features
- B01D15/24—Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to the treatment of the fractions to be distributed
Landscapes
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Treatment Of Liquids With Adsorbents In General (AREA)
Abstract
The present invention relates to technical field of bioengineering,Specifically,One kind matches somebody with somebody liquid layer analysis system online,Including a chromatographic column and a bubble trap,There are the system eight filling openings to be arranged to filling opening one to filling opening eight,Filling opening two,Mixed liquor is entered after the effect of precision plunger pump after solution mixing in filling opening three and filling opening four and fills internal,Filling opening five,Solution in filling opening six and filling opening seven all enters mixed liquor after the effect of precision plunger pump and fills internal,Solution in filling opening one enters directly into mixed liquor and fills internal,Solution in mixed liquor filling is by the solution mixing in the effect of three pump head high-pressure diaphragm pumps and the filling opening eight after three pump head autoclave diaphragm pumping actions,Solution after remixing is entered after heat exchanger and duplex filter in bubble trap,Entered back into after chromatographic column by the mixed solution after bubble trap and flowed out again from four liquid outlets.
Description
Technical field
The present invention relates to technical field of bioengineering, is that one kind matches somebody with somebody liquid layer analysis system online specifically.
Background technology
Chromatography (chromatography) is the abbreviation of " chromatographic analysis ".To be more using the difference of each component physical property
Component mixture is separated and method for measuring.There is two kinds of adsorption chromatography, Partition Chromatography.It is generally used for organic compound, gold
Belong to the analysis of ion, amino acid etc..Chromatography is reached point using material allocation proportion different between stationary phase and mobile phase
From the technology of purpose.The separation to the mixture of the complicated organic matter such as large biological molecule such as protein and nucleic acid is chromatographed to analyze
There is high resolving power.
Most of tomographic system needs very big mother liquor tank and requires accurate mother liquid concentration currently on the market, this design
It can meet that client completes precisely to match somebody with somebody liquid in the case where providing the mother liquor of smaller mother liquor tank and various concentrations, and it can not be met
Precisely match somebody with somebody liquid in the case of him.
The content of the invention
Based on above-mentioned technical problem, the present invention discloses one kind to match somebody with somebody liquid layer analysis system online, can pass through density flowmeters
The organic phase flow of high concentration is fed back, so as to accurately reach with liquid effect.
The concrete technical scheme that the present invention uses is as follows：
One kind matches somebody with somebody liquid layer analysis system, including a chromatographic column and a bubble trap online, and the system has eight liquid feedings
Mouth is arranged to filling opening one to filling opening eight, by precision after the solution mixing in filling opening two, filling opening three and filling opening four
Mixed liquor is entered after the effect of plunger pump and fills internal, the solution in filling opening five, filling opening six and filling opening seven is all by essence
Mixed liquor is entered after the effect of close plunger pump and fills internal, the solution in filling opening one enters directly into mixed liquor and fills internal, mixes
Close effect of the solution by three pump head high-pressure diaphragm pumps in liquid filling and the filling opening after three pump head autoclave diaphragm pumping actions
Solution mixing in eight, the solution after remixing are entered after heat exchanger and duplex filter in bubble trap, pass through gas
Mixed solution after bubble trap is entered back into after chromatographic column to flow out from four liquid outlets again.
Further improvement of the present invention, in addition to 3 Density feedback formula flowmeters, 3 PH detectors, 3 pressure sensings
Device, 1 UV detector, 1 air borne sensor and 3 electric conductivity detectors；2 in Density feedback formula flowmeter are arranged on three
Behind pump head high-pressure diaphragm pump, 1 is arranged between air trap and chromatographic column in addition；1 is arranged on and changes in PH detector
Between hot device and air trap, 1 is arranged between air trap and chromatographic column, 1 be arranged on chromatographic column and liquid outlet it
Between；1 in pressure sensor is arranged between heat exchanger and air trap, 1 be arranged on air trap and chromatographic column it
Between, 1 is arranged between chromatographic column and liquid outlet；UV detectors are arranged between chromatographic column and liquid outlet；Air borne sensor is set
Put between heat exchanger and air trap；1 in electric conductivity detector is arranged between heat exchanger and air trap, and 1 sets
Put between air trap and chromatographic column, 1 is arranged between chromatographic column and liquid outlet.
Further improvement of the present invention, filling opening two to filling opening six are provided with magnetic valve, filling opening one, liquid feeding afterwards
Pneumatic ball valve is provided with after mouth seven and filling opening eight.
Further improvement of the present invention, the solution in filling opening one are WFI solution, and the solution in filling opening two is molten for HAC
Liquid, the solution in filling opening three are HCl solution, and the solution in filling opening four is HCl solution, and the solution in filling opening five is
NaOH solution, the solution in filling opening six are ammonium acetate solution, and the solution in filling opening seven is NaCl solution, in filling opening eight
Solution be acetonitrile or ethanol.
Further improvement of the present invention, the connection between all parts use sanitation-grade clamp connection mode.
Beneficial effects of the present invention：The present invention can utilize density to calculate the amount that can accurately calculate required organic phase；Density
Flowmeter output signal, then by pump coutroi velocity, flow speed can be drawn rapidly
Brief description of the drawings
Fig. 1 is the structural representation of the present invention.
Embodiment
In order to deepen the understanding of the present invention, the present invention is done below in conjunction with drawings and examples and further retouched in detail
State, the embodiment is only used for explaining the present invention, and protection scope of the present invention is not formed and limited.
Embodiment：As shown in figure 1, a kind of match somebody with somebody liquid layer analysis system, including a chromatographic column and a bubble trap online,
There are the system eight filling openings to be arranged to filling opening one to filling opening eight, and the solution in filling opening one is WFI solution, liquid feeding
Solution in mouthfuls two is HAC solution, and the solution in filling opening three is HCl solution, and the solution in filling opening four is HCl solution, is added
Solution in liquid mouth five is NaOH solution, and the solution in filling opening six is ammonium acetate solution, and the solution in filling opening seven is NaCl
Solution, the solution in filling opening eight are acetonitrile or ethanol, after the solution mixing in filling opening two, filling opening three and filling opening four
Mixed liquor is entered after the effect of precision plunger pump and fills internal, the solution in filling opening five, filling opening six and filling opening seven
Mixed liquor is all entered after the effect of precision plunger pump and fills internal, the solution in filling opening one enters directly into mixed liquor
Fill internal, the solution in mixed liquor filling is by the effect of three pump head high-pressure diaphragm pumps and by three pump head autoclave diaphragm pumping actions
Solution mixing in filling opening eight afterwards, filling opening two to filling opening six are provided with magnetic valve, filling opening one, filling opening afterwards
Seven and filling opening eight after be provided with pneumatic ball valve, the solution after remixing enters gas after heat exchanger and duplex filter
Steep in trap, entered back into after chromatographic column by the mixed solution after bubble trap and flowed out again from four liquid outlets.
In the present embodiment, in addition to 3 Density feedback formula flowmeters, 3 PH detectors, 3 pressure sensors, 1
UV detectors, 1 air borne sensor and 3 electric conductivity detectors；2 in Density feedback formula flowmeter are arranged on three pump heads height
Press behind membrane pump, 1 is arranged between air trap and chromatographic column in addition；In PH detector 1 be arranged on heat exchanger and
Between air trap, 1 is arranged between air trap and chromatographic column, and 1 is arranged between chromatographic column and liquid outlet；Pressure
1 in force snesor is arranged between heat exchanger and air trap, and 1 is arranged between air trap and chromatographic column, and 1
It is individual to be arranged between chromatographic column and liquid outlet；UV detectors are arranged between chromatographic column and liquid outlet；Air borne sensor is arranged on
Between heat exchanger and air trap；1 in electric conductivity detector is arranged between heat exchanger and air trap, and 1 is arranged on
Between air trap and chromatographic column, 1 is arranged between chromatographic column and liquid outlet；Connection between all parts, which uses, to be defended
Raw level clamp connection mode.
General principle, the main features and advantages of the present invention have been shown and described above.The technical staff of the industry should
Understand, the present invention is not limited to the above embodiments, the original for simply illustrating the present invention described in above-described embodiment and specification
Reason, without departing from the spirit and scope of the present invention, various changes and modifications of the present invention are possible, and these change and changed
Enter all fall within the protetion scope of the claimed invention.The claimed scope of the invention is by appended claims and its equivalent
Thing defines.
Claims (5)
1. one kind matches somebody with somebody liquid layer analysis system online, it is characterised in that including a chromatographic column and a bubble trap, the system has
Eight filling openings are arranged to filling opening one to filling opening eight, and the solution in the filling opening two, filling opening three and filling opening four mixes
Mixed liquor is entered after the effect of precision plunger pump fill internal after conjunction, the filling opening five, filling opening six and filling opening seven
Interior solution all enters mixed liquor after the effect of precision plunger pump and fills internal, and the solution in the filling opening one directly enters
Enter to mixed liquor and fill internal, the solution in the mixed liquor filling is by the effect of three pump head high-pressure diaphragm pumps and high by three pump heads
The solution mixing in the filling opening eight after barrier film pumping action is pressed, the solution after remixing passes through heat exchanger and duplex filter is laggard
Enter into bubble trap, entered back into by the mixed solution after the bubble trap after the chromatographic column again from four liquid outlet streams
Go out.
2. according to claim 1 match somebody with somebody liquid layer analysis system online, it is characterised in that also including 3 Density feedback formula flows
Meter, 3 PH detectors, 3 pressure sensors, 1 UV detector, 1 air borne sensor and 3 electric conductivity detectors；It is described close
2 in degree reaction type flowmeter are arranged on behind the three pump heads high-pressure diaphragm pump, and 1 is arranged on the air and falls into addition
Between trap and the chromatographic column；1 is arranged between the heat exchanger and the air trap in the PH detector, and 1 sets
Put between the air trap and the chromatographic column, 1 is arranged between the chromatographic column and the liquid outlet；The pressure
1 in force snesor is arranged between the heat exchanger and the air trap, and 1 is arranged in the air trap and institute
Between stating chromatographic column, 1 is arranged between the chromatographic column and the liquid outlet；The UV detectors are arranged on the chromatographic column
Between the liquid outlet；The air borne sensor is arranged between the heat exchanger and the air trap；The conductance inspection
1 surveyed in device is arranged between the heat exchanger and the air trap, and 1 is arranged in the air trap and the layer
Between analysing post, 1 is arranged between the chromatographic column and the liquid outlet.
3. according to claim 2 match somebody with somebody liquid layer analysis system online, it is characterised in that the filling opening two to filling opening six it
After be provided with magnetic valve, be provided with pneumatic ball valve after the filling opening one, filling opening seven and filling opening eight.
4. match somebody with somebody liquid layer analysis system online according to claim any one of 1-3, it is characterised in that in the filling opening one
Solution is WFI solution, and the solution in the filling opening two is HAC solution, and the solution in the filling opening three is HCl solution, institute
It is HCl solution to state the solution in filling opening four, and the solution in the filling opening five is NaOH solution, molten in the filling opening six
Liquid is ammonium acetate solution, and the solution in the filling opening seven is NaCl solution, and the solution in the filling opening eight is acetonitrile or second
Alcohol.
5. according to claim 4 match somebody with somebody liquid layer analysis system online, it is characterised in that the connection between all parts, which uses, to be defended
Raw level clamp connection mode.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710435890.2A CN107715498A (en) | 2017-06-09 | 2017-06-09 | One kind matches somebody with somebody liquid layer analysis system online |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710435890.2A CN107715498A (en) | 2017-06-09 | 2017-06-09 | One kind matches somebody with somebody liquid layer analysis system online |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN107715498A true CN107715498A (en) | 2018-02-23 |
Family
ID=61201080
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710435890.2A Pending CN107715498A (en) | 2017-06-09 | 2017-06-09 | One kind matches somebody with somebody liquid layer analysis system online |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN107715498A (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108745022A (en) * | 2018-05-31 | 2018-11-06 | 上海药明生物技术有限公司 | A kind of buffer system based on AKTA avant |
| CN108970177A (en) * | 2018-06-11 | 2018-12-11 | 中国科学院过程工程研究所 | A kind of chromatographic column with bubble trap |
| CN113663364A (en) * | 2020-05-14 | 2021-11-19 | 佛山汉腾生物科技有限公司 | Chromatography device |
| CN113663365A (en) * | 2020-05-14 | 2021-11-19 | 佛山汉腾生物科技有限公司 | Bubble trap mechanism and chromatography system |
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| JPH06238143A (en) * | 1993-02-15 | 1994-08-30 | Mitsubishi Heavy Ind Ltd | Liquid mixing device |
| US6627075B1 (en) * | 1999-03-30 | 2003-09-30 | Agilent Technologies, Inc. | Device for and process of preparing volume flows of liquids in at least one passage of a chromatographic column |
| CN101851815A (en) * | 2009-10-14 | 2010-10-06 | 中国石油化工股份有限公司 | Method for removing iron ions from acrylic fiber solvent by adsorptive separation |
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-
2017
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108745022A (en) * | 2018-05-31 | 2018-11-06 | 上海药明生物技术有限公司 | A kind of buffer system based on AKTA avant |
| CN108970177A (en) * | 2018-06-11 | 2018-12-11 | 中国科学院过程工程研究所 | A kind of chromatographic column with bubble trap |
| CN108970177B (en) * | 2018-06-11 | 2020-04-14 | 中国科学院过程工程研究所 | Chromatographic column with bubble trap |
| CN113663364A (en) * | 2020-05-14 | 2021-11-19 | 佛山汉腾生物科技有限公司 | Chromatography device |
| CN113663365A (en) * | 2020-05-14 | 2021-11-19 | 佛山汉腾生物科技有限公司 | Bubble trap mechanism and chromatography system |
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| PB01 | Publication | ||
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Application publication date: 20180223 |
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| RJ01 | Rejection of invention patent application after publication |