CN113648393A

CN113648393A - Fermented traditional Chinese medicine composition for preventing salpingitis of laying hens and improving laying performance of laying hens

Info

Publication number: CN113648393A
Application number: CN202110842748.6A
Authority: CN
Inventors: 苏建青; 褚秀玲; 付祥; 尹薪项; 郑文浩; 卢玉谭; 陈世伟
Original assignee: Hebei Keweixian Biotechnology Co ltd; Liaocheng University
Current assignee: Hebei Keweixian Biotechnology Co ltd; Liaocheng University
Priority date: 2021-07-26
Filing date: 2021-07-26
Publication date: 2021-11-16

Abstract

The invention provides a fermented traditional Chinese medicine composition for preventing salpingitis of laying hens and improving the production performance of the laying hens, which comprises the following raw material components: herba Leonuri, radix astragali, herba Taraxaci, radix Angelicae sinensis, rhizoma Ligustici Chuanxiong, radix rehmanniae Preparata, folium Artemisiae Argyi, fructus Ligustri Lucidi, herba Portulacae, Curcuma rhizome and radix Acanthopanacis Senticosi; and (3) auxiliary material part: rice chaff, bean pulp, corn and stone powder; strain part: lactic acid bacteria and bacillus subtilis; the fermented traditional Chinese medicine composition has obvious effects of preventing and treating salpingitis of laying hens, improving the laying rate of the laying hens and enhancing the quality of eggs, and provides possibility for enhancing the production performance of the laying hens, improving the laying rate of the laying hens, improving the quality of eggshells and preventing and treating the salpingitis.

Description

Fermented traditional Chinese medicine composition for preventing salpingitis of laying hens and improving laying performance of laying hens

Technical Field

The invention relates to a fermented traditional Chinese medicine composition for preventing salpingitis of laying hens and improving the production performance of the laying hens, belonging to the technical field of veterinary medicines.

Background

The laying hens are a main breeding direction of the chicken industry, the laying rate and the egg quality are mainly improved when the laying hens are bred, the yield and the quality of chicken are not improved, the eggs are a main income source for breeding the laying hens, and the egg consumption is very large in China every year. In the nutrition world, eggs have been always named as ' full-nutrition food ', and are very honored by ' the most-nutrition breakfast ' in the world ' in magazines and even for eggs. In the traditional Chinese medicine, the eggs have the effects of nourishing yin, moistening dryness, tonifying heart, calming mind, nourishing blood, preventing miscarriage, detoxifying, relieving itching and the like. With the increase of income and living standard of residents, expectations and requirements of consumers on the safety, nutrition and the like of animal products are higher and higher, food with high safety and excellent quality in the market is favored by more consumers, and the demand of people on eggs is also increased from full demand to quality demand.

The quality indexes of eggs mainly comprise external quality and internal quality, including eggshell condition, weight of the eggs, eggshell strength, eggshell thickness, egg shape index, eggshell color, specific gravity of the eggs, surface cleanliness and the like, the quality indexes are conventional indexes reflecting the quality of the eggs, and the eggshell quality of the eggs is also an important standard for measuring the quality of the eggs.

The traditional Chinese medicine has a long fermentation history in China, and the essence of the traditional Chinese medicine fermentation processing is that the biological transformation of the medicinal materials is utilized, the medicinal properties of the medicines can be changed, the effects are enhanced and the toxicity is reduced through the fermentation, so that the pharmacological action of the medicinal materials is changed to generate new chemical components. Compared with other traditional Chinese medicine processing methods such as stewing, frying, calcining, processing, refining, baking and the like, the fermentation method is carried out under the conditions of normal temperature and normal pressure, the reaction condition is mild, the operation is simple, meanwhile, the enzyme generated in the microbial growth and metabolism process is utilized in the conversion reaction, and the enzyme selectively carries out structural modification on the substrate, so that the reaction which cannot be realized by other processing methods can be carried out, and the traditional Chinese medicine components with better activity and more reasonable structure are obtained with the advantages of low cost and less public hazard.

Many Chinese herbal medicines have the effects of improving the laying rate of laying hens, resisting bacteria and inflammation, enhancing immunity, resisting stress, reducing the feed-egg ratio and the cholesterol content in egg yolks, improving the quality and the taste of egg products and the like, are non-toxic or low-toxicity, do not pollute the environment, have no medicine residues, have wide medicine sources and low price, can obviously reduce the generation of soft shell eggs, malformed eggs and preserved eggs and the breakage of the eggs, and the fermented traditional Chinese medicines are rapidly developed in the veterinary medicine industry in two years due to the characteristics of quick absorption, strong medicine effect, no toxic or side effect and the like, and become a key research and development project of 'resistance reduction/resistance replacement'. Especially under the new condition of 'resistance reduction' or even 'no resistance' cultivation, the incidence rate of bacterial diseases shows an increasing trend, and the oophoritis, salpingitis and peritonitis are common diseases which are easy to occur to chickens, especially laying hens, and are clinically characterized by the inflammation of ovaries, oviducts and peritoneum, and the follicular distortion, hyperemia and hemorrhage are serious. The etiology of the disease is complex, so the disease is a frequently encountered disease threatening the healthy development of the chicken industry.

However, the modern Chinese medicine fermentation technology in China is still in the beginning stage, the varieties on the market are few, a plurality of problems need to be solved, the traditional Chinese medicine is fermented by using a single strain, and the research on mixed strains is less; the 11146 medicinal plants in China exist, but the varieties of fermentation research of the traditional Chinese medicines are few, which is related to the complexity of the traditional Chinese medicine components and unclear drug effect and active ingredient metabolic pathways; the microbial metabolite and the effective components of the traditional Chinese medicine may have a neutralization effect, so that the biological activity of the traditional Chinese medicine is reduced, and the biological activity of a plurality of traditional Chinese medicines after fermentation is not obviously changed, even the pharmacological activity is reduced, namely the traditional Chinese medicines have different compositions and different used microorganisms, and the effect generated after the traditional Chinese medicines are fermented cannot be expected, so that the development and the application of the traditional Chinese medicine fermented product are limited.

Disclosure of Invention

Aiming at the defects of the prior art, the invention provides a fermented traditional Chinese medicine composition for preventing and treating salpingitis of laying hens and improving the production performance of the laying hens. Provides basis for the fermentation technology of the Chinese herbal compound, and promotes the development of the fermented Chinese herbal medicine and the economic benefit of the fermented Chinese herbal medicine industry.

The fermentation method of the medicine compound of the invention is used for improving the content of the effective components of the fermented traditional Chinese medicine compound, so that the fermented traditional Chinese medicine compound can enhance the production performance of laying hens, improve the laying rate of laying hens, improve the quality of eggshells and prevent salpingitis.

A fermented traditional Chinese medicine composition for preventing and treating salpingitis of laying hens and improving the production performance of the laying hens comprises the following raw material components:

the traditional Chinese medicine part: herba Leonuri, radix astragali, herba Taraxaci, radix Angelicae sinensis, rhizoma Ligustici Chuanxiong, radix rehmanniae Preparata, folium Artemisiae Argyi, fructus Ligustri Lucidi, herba Portulacae, Curcuma rhizome and radix Acanthopanacis Senticosi;

and (3) auxiliary material part: rice chaff, bean pulp, corn and stone powder;

strain part: lactic acid bacteria and Bacillus subtilis.

According to the invention, the raw material components of the traditional Chinese medicine composition comprise the following components in parts by weight:

the traditional Chinese medicine part: 190 parts of motherwort 160-;

and (3) auxiliary material part: 220 parts of rice chaff, 15-25 parts of bean pulp, 170 parts of corn bran and 130-120 parts of stone powder;

strain part: 550 portions of lactobacillus liquid 400 plus, the strain concentration is more than or equal to 10⁷cfu/(ml or g), 400 portions of bacillus subtilis liquid and 550 portions, wherein the strain concentration is more than or equal to 10⁷cfu/(ml or g).

Further preferably, the traditional Chinese medicine composition comprises the following raw material components in parts by weight:

the traditional Chinese medicine part: 182 parts of motherwort, 182 parts of astragalus, 159 parts of dandelion, 136 parts of angelica, 136 parts of ligusticum wallichii, 136 parts of prepared rehmannia root, 136 parts of folium artemisiae argyi, 114 parts of glossy privet fruit, 114 parts of purslane, 114 parts of turmeric and 91 parts of acanthopanax;

and (3) auxiliary material part: 210 parts of rice chaff, 20 parts of soybean meal, 160 parts of corn and 110 parts of stone powder;

a bacterial liquid part: 500 parts of lactobacillus bacterial liquid with the bacterial liquid concentration of 10⁸cfu/(ml or g) or more; 500 parts of bacillus subtilis liquid with the concentration of 10⁸cfu/(ml or g) or more; the lactobacillus comprises enterococcus faecalis and lactobacillus acidophilus, and the composition proportion of the enterococcus faecalis to the lactobacillus acidophilus is 1: 1.

more preferably, the bacterial species number of the enterococcus faecalis is CICC23658, the bacterial species number of the lactobacillus acidophilus is CICC22162, and the bacterial species number of the bacillus subtilis is CICC 20872.

The preparation method of the traditional Chinese medicine composition comprises the following steps:

(1) preparing lactobacillus liquid and bacillus subtilis liquid;

(2) uniformly mixing the lactobacillus liquid and the bacillus subtilis liquid in the step (1) with the traditional Chinese medicine part and the auxiliary material part to prepare a solid fermentation material;

(3) and (3) performing solid fermentation on the solid fermentation material in the step (2) in a sealed container to obtain the fermented traditional Chinese medicine composition.

According to the invention, the grinding mesh number of the traditional Chinese medicine part and the auxiliary material part in the step (2) is preferably 40 meshes.

According to the invention, the solid fermentation material in the step (2) has a water content of 40-42%.

Preferably, in step (3), the solid fermentation material is subjected to solid fermentation in a sealed bag under the conditions of 20-25 ℃ and a fermentation period of 18-25 days.

The use of the above pharmaceutical composition in poultry farming.

The application of the pharmaceutical composition in feeding chickens.

Pharmacological analysis

Motherwort: bitter and pungent in flavor and slightly cold in nature. It enters liver, pericardium and bladder meridians. Has the effects of promoting blood circulation, regulating menstruation, inducing diuresis, relieving swelling, and clearing away heat and toxic materials.

Contains leonurine, stachydrine, leonuridine, leonurenine, linolenic acid, beta-linolenic acid, lauric acid, oleic acid, benzoic acid, rutin, fumaric acid, sterol, vitamin A, and the like, and also contains arginine, 4-guanidino-1-butanol, 4-guanidino-butyric acid and stachyose.

Astragalus root: sweet in flavor and slightly warm in nature. It enters spleen and lung meridians. Has the effects of invigorating qi, consolidating exterior, expelling toxin, expelling pus, promoting urination, and promoting granulation.

Radix astragali comprises flavone and saponin. The flavone components include formononetin, 3 '-hydroxy formononetin and its glucoside, 2', 3 '-dihydroxy-7, 4' -dimethoxy isoflavone, 7, 2 '-dihydroxy-3', 4 '-dimethoxy isoflavan and its glucoside, 7, 3' -dihydroxy-4 ', 5' -dimethoxy isoflavan, 3-hydroxy-9, 10-dimethoxy rosewood alkane and its glucoside, etc.

Dandelion: cold in nature, bitter and sweet in taste. It enters liver meridian and stomach meridian. Has the effects of clearing away heat and toxic materials, promoting urination and resolving hard mass.

Contains taraxasterol, choline, inulin, pectin, taraxasterol, taraxacin, caffeic acid, etc.

Chinese angelica: sweet and pungent in flavor and warm in nature. It enters liver, heart and spleen meridians. Has the functions of enriching blood, promoting blood circulation, regulating menstruation, relieving pain, moistening intestine and relaxing bowels.

The angelica contains various amino acids, and the total content is 6.5 percent. Wherein the content of arginine is about one time higher than other amino acids. The angelica further contains vitamin B12 with content of 0.25-0.4mg/100g, vitamin A, vitamin E and the like. The content of volatile oil in radix Angelicae sinensis can reach 0.2% -0.4%, and the volatile oil has acidic, phenolic and neutral parts, respectively 5.88%, 3.46% and 90.4%. The volatile oil contains ligustilide 45%, n-butylidenephthalide 11.3%, and has specific fragrance. The angelica contains ferulic acid, succinic acid, nicotinic acid, uracil, adenine, stigmasterol, D-glucoside, tetradecanol-1, vanillic acid and gelsemium elegans.

Ligusticum wallichii: pungent flavor and warm nature. It enters liver, gallbladder and pericardium meridians. Has the effects of promoting blood circulation, activating qi-flowing, dispelling pathogenic wind and relieving pain. The rhizome of Sichuan lovage is pungent, warm, fragrant and dry, and can be scattered and ascended to the vertex; it enters the blood system and goes down to reach the blood sea.

Contains phenolic substances such as alkaloid and ferulic acid, and also contains ligustrazine, ligustilide, ligustrazine, trimethylamine hydrochloride, choline hydrochloride, etc. In addition, the rhizome of Ligusticum chuanxiong contains neutral oil, which comprises pentadecane, hexadecane, heptadecanoic acid ethyl ester, isoheptadecanoic acid ethyl ester, and isoheptadecanoic acid methyl ester.

Prepared rehmannia root: sweet in flavor and slightly warm in nature. Enter liver and kidney meridians. Has effects in nourishing yin and tonifying blood.

Prepared rehmannia root contains a small amount of cycloolefinphenyl ether compounds, and has been isolated to obtain: leonurin, aucubin, catalpol, digitonin A, B, C, D, merlinoside 1, lutein A, D, rehmannia root chloroforminal glycoside 2, etc. And containing monoterpene component: pyrogallol A, B, C, pyrogallol A, B, C, pyrogallol 2, digitoxin 3, etc. And amino acids, the composition of which is lysine-free and the content of which is reduced compared with that of dried rehmannia. Also contains saccharide, wherein the content of monosaccharide is more than two times of that in fresh rehmannia root by 5. And also contains trihydroxy-beta-ionone, 5-hydroxyaegineticacid 3, succinic acid, 5-oxyproline, 5-hydroxymethyl furoic acid, uracil), uracil, uridine 2, etc. And separating from the petroleum ether extract: oleic acid, palmitic acid, stearic acid, arachidic acid, pentadecanoic acid, palmitoleic acid, endo-myristic acid, nonadecanoic acid, heneicosanoic acid, and dicetyl-6.

Folium artemisiae argyi: pungent and bitter with warm nature. It enters liver, spleen and kidney meridians. Has the effects of warming channels, stopping bleeding, dispelling cold and relieving pain; externally used for eliminating dampness and relieving itching.

The mugwort contains volatile oil, is a multi-component mixture, and is identified by separation as follows: terpinenol-4, beta-caryophyllene, artesunol, linalool, camphor, borneol, eucalyptol, phellandrene, piperylene, thujaol, etc.

Glossy privet fruit: mild in nature, sweet and bitter in taste. Belongs to the liver and kidney channels, and has the effects of nourishing yin, prolonging life, tonifying liver and kidney, clearing heat, improving eyesight, blackening hair and beard, and the like.

Contains oleanolic acid, acetyl oleanolic acid, ursolic acid acetate, p-hydroxyphenylethanol, 3, 4-dihydroxyphenylethanol, beta-sitosterol, mannitol, racemic-eriodictyol, dextro-taxifolin, quercetin, ligustrin, 10-hydroxyligustrin, ligustrin, oleuropein, 10-hydroxyoleuropein, p-hydroxyphenylethyl-beta-D-glucoside, 3, 4-dihydroxyphenethyl-beta-glucoside, methyl-alpha-D-galactopyranoside, syringol glycoside, neoligustrin, ligustric acid, oleuropein and secoiridoid glycoside with the code of GI-3. It also contains polysaccharide composed of rhamnose, arabinose, glucose, and fucose, and 7 phospholipid compounds with a total amount of 0.39%, wherein the content of phosphatidylcholine is highest and accounts for 56.52% + -1.34%. And contains 11 elements of potassium, calcium, magnesium, sodium, zinc, iron, manganese, copper, nickel, chromium, silver and the like, wherein the copper, the iron, the zinc, the manganese, the chromium and the nickel are necessary trace elements.

Purslane: sour in taste and cold in nature. It enters liver and large intestine meridians. The whole herb is used for medicine, and has the effects of clearing heat and promoting diuresis, detoxifying and reducing swelling, diminishing inflammation, quenching thirst and promoting urination; the seeds can improve eyesight.

The purslane contains rich dihydroxyethylamine, malic acid, glucose, calcium, phosphorus, iron, vitamin E, carotene, vitamin B, vitamin C and other nutrient substances.

Turmeric: pungent and bitter with warm nature. It enters spleen and liver meridians. Has the functions of breaking blood, promoting qi circulation, dredging meridian and relieving pain.

The stem contains curcumin compounds: curcumin, p, bisdemethoxycurcumin, p-hydroxycinnamoyl feruloylmethane, i.e. demethoxycurcumin, dihydrocurcumin; sesquiterpene compounds: curcumone, curcumol A, B, geranylgeraniol-13 aldehyde, 4-hydroxytryptomyrrha-2, 10-dien-9-one, 4-methoxy-5-hydroxytryptomyrrha-2, 10-dien-9-one, 2, 5-dihydroxy-myrrha-3, 10-diene, protocurcumenol, curdione, dehydrocurdione, alpha-turmerone, curcumenol, curenol, isocurenol, curdione, protocurenol, epicurenol, 4, 5-dihydroxy-myrrha-2, 10-diene; acid polysaccharide: turmeric polysaccharide A, B, C, D. The volatile oil (4.2%) contains curcumone, aromatic-curcumone, curcumene, geranylgeranone, aromatic-curcumene, eucalyptol, terpinene, curcumenol, curolene, curdione, alpha-pinene, beta-pinene, limonene, linalool, caryophyllene, borneol, etc. Also contains campesterol, stigmasterol, beta-sitosterol, cholesterol, fatty acid, and metal elements such as potassium, sodium, magnesium, calcium, manganese, iron, copper, zinc, etc.

Acanthopanax root: pungent, slightly bitter and warm. It enters spleen, kidney and heart meridians. Has effects of invigorating kidney, strengthening waist, invigorating qi, tranquilizing mind, promoting blood circulation, and dredging collaterals.

The root contains eleutheroside A, B, B1, C, D, E, eleutheroside A is daucosterol, and eleutheroside B is syringin; the root also contains sinapinal glucoside, coniferyl glucoside, liriodendrin, amygdalin, and 28-epoxy oleanane.

The fermented traditional Chinese medicine composition for preventing and treating salpingitis of the laying hens and improving the production performance of the laying hens is prepared by performing syndrome differentiation of wei-defensive qi, ying-nutrient blood and six meridians on intestinal diseases under the guidance of the traditional Chinese medicine theory of typhoid fever and the warm disease theory of warm diseases, and combining the medicine property and meridian tropism of the traditional Chinese medicine on the basis of a traditional prescription. In the formula, the motherwort herb is bitter and pungent, is slightly cold, enters liver, heart and bladder channels, has the effects of activating blood circulation to dissipate blood stasis, inducing diuresis to alleviate edema, and clearing heat and removing toxicity, and is a monarch drug. Rhizoma Ligustici Chuanxiong has effects of promoting qi circulation, resolving stagnation, promoting blood circulation and relieving pain; the angelica sinensis can enrich and activate blood, and promote blood circulation to stop pain; radix astragali has effects of invigorating qi, invigorating yang, invigorating qi, and consolidating superficial resistance; radix rehmanniae Preparata has effects of nourishing yin and tonifying blood; fructus Ligustri Lucidi has effects in nourishing liver and kidney; acanthopanax senticosus has the effects of tonifying qi, invigorating spleen and tonifying kidney. The six medicines are used as ministerial medicines. The dandelion has the functions of clearing away heat and toxic materials; folium Artemisiae Argyi has effects of warming channels, stopping bleeding, dispelling cold, and relieving pain; herba Portulacae has effects of clearing away heat and toxic materials, cooling blood, stopping bleeding, and relieving dysentery; turmeric root tuber, rhizoma Curcumae Longae, acts to break blood, promote the circulation of qi, dredge meridians and relieve pain. The four medicines are used as adjuvant and guiding medicines. The compatibility of the prescription is characterized in that tonics are added into the blood-activating and stasis-removing medicines, so that the aim of expelling evil factors and strengthening body resistance is achieved, and the prescription is one of the main principles of Chinese veterinarians for treating diseases.

Advantageous effects

The fermented traditional Chinese medicine composition for preventing and treating laying hen salpingitis and improving laying performance of laying hens, which is prepared by the invention, has obvious effects on preventing and treating laying hen salpingitis, improving laying rate of laying hens and enhancing egg quality, and provides possibility for enhancing the laying performance of laying hens, improving the laying rate of laying hens, improving eggshell quality and preventing and treating salpingitis.

Detailed Description

The present invention is further illustrated with reference to the following examples, but the scope of the present invention is not limited thereto.

The details not described in the examples are according to the state of the art.

Sources of materials

Motherwort, astragalus, dandelion, angelica, ligusticum wallichii, prepared rehmannia root, folium artemisiae argyi, glossy privet fruit, purslane, turmeric and acanthopanax, and is purchased from a chat about Wanquan pharmacy.

The strain numbers of enterococcus faecalis, lactobacillus acidophilus and bacillus subtilis are CICC23658, CICC22162 and CICC20872 respectively, and all come from China center for culture collection of industrial microorganisms.

Example 1

A fermented traditional Chinese medicine composition for preventing and treating salpingitis of laying hens and improving production performance of the laying hens comprises the following raw material medicines in parts by weight:

a bacterial liquid part: the lactobacillus strain liquid comprises 500 parts of lactobacillus liquid and 500 parts of bacillus subtilis CICC20872 liquid, the lactobacillus comprises enterococcus faecalis CICC23658 and lactobacillus acidophilus CICC22162, and the proportion of the enterococcus faecalis to the lactobacillus acidophilus is 1: 1.

1. separating and purifying strains

(1) Preparation of sample dilution solution

Weighing strain sample1g, put into a triangular flask containing 99ml of sterile water, vibrate and mix uniformly to mix the sample with water, and disperse the cells. Sucking 1ml of suspension from a 1ml sterile pipette, adding into a test tube containing 9ml of sterile water, mixing, sucking 1ml of suspension from the test tube with a sterile pipette, adding into another test tube containing 9ml of sterile water, mixing, and repeating the steps to obtain 10^-1，10^-2，10^-3，10^-4，10^-5，10^-6Solutions of different dilutions (dilution factor was determined by strain content).

(2) Coating of

Respectively provided with a sterile suction pipe 10^-4，10^-5，10^-6And (3) taking 0.2ml of each three-tube diluent, putting the three-tube diluent into a plate with written dilution, slightly and uniformly coating the surface of the culture medium by using a sterile glass coating rod, and standing for 5-10 min at room temperature to enable the bacterial liquid to be adsorbed into the culture medium.

(3) Culturing

Placing in a greenhouse incubator at 37 ℃, culturing for 24h,

(4) strain isolation

Flat plate scribing

Picking single colony with inoculating needle, streaking, inoculating to plate culture medium, culturing in 37 deg.c greenhouse culture box for 24 hr.

(5) Separating and purifying strains

Separating and purifying lactobacillus acidophilus CICC22162, enterococcus faecalis CICC23658 and bacillus subtilis CICC20872, and storing on slant for use.

2. Seed liquid culture

(1) Lactic acid bacteria culture medium: LB medium, 2500ml medium according to the formulation.

(2) The bacillus subtilis culture medium is 2500ml prepared according to the formula.

(3) The prepared culture medium is bottled and sterilized by high pressure steam at 121 ℃ for 25 minutes.

(4) The sterilized culture medium is placed at about 30 ℃ for inoculation.

(5) Placing the inoculated bacillus subtilis culture medium into a shaking table, wherein the culture conditions are as follows: culturing at 36 deg.C and 150rpm/min for 24h to obtain Bacillus subtilis seed solution; and (3) placing the lactic acid bacteria culture medium in a shaking table for culturing at the temperature of 30 ℃ for 24h, and shaking once every 6h to prepare lactic acid bacteria seed liquid, namely enterococcus faecalis seed liquid and lactobacillus acidophilus seed liquid.

Preparation of the Medium

The formula of the lactic acid bacteria culture medium (LB culture medium) is shown in Table 1;

TABLE 1

Serial number	Name (R)	Quantity/kg
			1	Peptone	4
2	Yeast powder	2
			3	Sodium chloride	4
4	Water (W)	400
			5	pH	6.2±0.2

The culture medium of Bacillus subtilis is shown in Table 2

TABLE 2

Serial number	Name (R)	Quantity/kg
			1	Bean pulp	2
2	Corn flour	1
			3	Dipotassium hydrogen phosphate	0.6
4	Potassium dihydrogen phosphate	0.3
			5	Calcium carbonate	0.1
6	Magnesium sulfate	0.04
			7	Sodium chloride	1
8	Glucose	0.2
			9	Water (W)	200
10	pH	6.5-7.0

3. Fermentation culture

(1) Respectively inoculating the seed liquid prepared in the step 2 into a fermentation tank, wherein the culture conditions of the lactic acid bacteria are as follows: rotating once at 30 deg.C for 30min, controlling pH at 6.0-6.5, and culturing for 48 hr; the culture conditions of the bacillus subtilis are as follows: the temperature is 36 ℃, the rotating speed is 150rpm/min, the pH value is controlled to be 6.5-7.0, the culture is carried out for 48 hours, and the thallus concentration in the prepared fermentation liquor is more than 10⁸cfu/ml。

4. Fermentation of solid materials

(1) Inoculating solid materials into the fermentation liquor prepared in the step 3, wherein the components of the solid fermentation materials are shown in a table 3;

TABLE 3

(2) Solid material inoculation: and uniformly mixing the fermentation liquor, the traditional Chinese medicine part and the auxiliary material part to complete solid material inoculation, and finally adjusting the water content of the inoculated solid material to be 40-42%.

(3) Filling the solid material inoculated in the step (2) into a sealed bag, wherein the size of the sealed bag is 35cm in length and 25cm in width, and the filling amount of each bag is 2.5 kg; maintaining the ambient temperature at 20-25 deg.C, and fermenting for 21 days to obtain fermented Chinese medicinal composition.

(4) Vacuum package

3kg of the fermented traditional Chinese medicine composition prepared in the step (3) is vacuumized and packaged, and is placed for 3 days; and the vacuum tightness is detected, so that bag expansion and mold growth are prevented.

Example 2

the traditional Chinese medicine part: 170 parts of motherwort, 170 parts of astragalus, 140 parts of dandelion, 140 parts of angelica, 140 parts of ligusticum wallichii, 140 parts of prepared rehmannia root, 140 parts of folium artemisiae argyi, 80 parts of glossy privet fruit, 80 parts of purslane, 80 parts of turmeric and 60 parts of acanthopanax;

the preparation method of the traditional Chinese medicine composition is the same as that of example 1.

Example 3

the traditional Chinese medicine part: 160 parts of motherwort, 160 parts of astragalus, 130 parts of dandelion, 130 parts of angelica, 130 parts of ligusticum wallichii, 130 parts of prepared rehmannia root, 130 parts of folium artemisiae argyi, 70 parts of glossy privet fruit, 70 parts of purslane, 70 parts of turmeric and 50 parts of acanthopanax;

Comparative example 1

The difference from the embodiment 1 is that the traditional Chinese medicine composition is prepared without motherwort, angelica, acanthopanax and astragalus root, and the rest is unchanged.

The traditional Chinese medicine part: 159 parts of dandelion, 136 parts of ligusticum wallichii, 136 parts of prepared rehmannia root, 136 parts of folium artemisiae argyi, 114 parts of glossy privet fruit, 114 parts of purslane and 114 parts of turmeric;

Comparative example 2

The difference from the embodiment 1 is that the traditional Chinese medicine part, the auxiliary material part and the bacterial liquid part are directly and uniformly mixed without fermentation treatment to prepare the traditional Chinese medicine composition.

The traditional Chinese medicine part: 182 parts of motherwort, 182 parts of astragalus, 159 parts of dandelion, 136 parts of angelica, 136 parts of ligusticum wallichii, 136 parts of prepared rehmannia root, 136 parts of folium artemisiae argyi, 114 parts of glossy privet fruit, 114 parts of purslane, 114 parts of turmeric and 91 parts of acanthopanax.

comparative example 3

A fermented traditional Chinese medicine composition for preventing and treating salpingitis of laying hens and improving the production performance of the laying hens is different from that in example 1 in the following specific steps:

a bacterial liquid part: 500 parts of a lactic acid bacteria liquid and 500 parts of a bacillus subtilis CICC20872 liquid, wherein the lactic acid bacteria is Lactobacillus acidophilus CICC22162, and does not include enterococcus faecalis CICC 23658.

Examples of effects

The detection method comprises the following steps:

(1) and (3) flavone content:

preparing an extracting solution: accurately weighing 10g of fermented traditional Chinese medicine, pouring into a conical flask with a plug, weighing 200ml of 75% ethanol with a 1000ml measuring cylinder, pouring into the conical flask, shaking up, and standing for 20 minutes to obtain the fermented traditional Chinese medicine composition extract.

Sucking 1.00 ml of extracting solution, placing the extracting solution in a 25ml volumetric flask, adding 1.00 ml of NaNO solution with the mass concentration of 5%, shaking up, standing for 6 minutes, adding 1.00 ml of Al (NO) solution with the mass concentration of 10%, shaking up, standing for 6 minutes, adding 5.00 ml of 4% NaOH solution, shaking up, finally adding 50% ethanol, fixing the volume to a scale mark, turning the volumetric flask upside down, standing for 15 minutes after the solution is mixed uniformly, measuring the absorbance at the wavelength of 510nm, and obtaining the mass concentration of the flavonoid substances in the solution according to a rutin standard curve.

(2) The content of saponin: weighing 3 parts of fermented traditional Chinese medicine composition, 10g of each part, adding 70% ethanol according to the material-liquid ratio of 1:15, performing ultrasonic treatment at 60 ℃ for 40min, performing suction filtration, combining three parts of filtrate, concentrating, extracting with 1 volume of water-saturated n-butanol for 2 times, combining upper layer n-butanol extract, performing reduced pressure concentration to dryness, adding methanol to dissolve, performing constant volume to 50m L to obtain fermented traditional Chinese medicine composition total saponin extract, filtering with a 0.45 mu m filter membrane for sterilization, and storing at 4 ℃ for later use. Taking astragaloside IV as standard, measuring absorbance values of the series of standard and the solution to be measured after dilution by proper times at 560nm, drawing a standard curve, and calculating to obtain total saponin concentration (mg/m L) according to regression equation and dilution times.

(3) Polysaccharide content: diluting 10mL of the extract of the fermented traditional Chinese medicine composition in the step (1) by 25 times, taking 0.5mL of the diluent, adding water to dilute to 2mL in a 25mL test tube with a plug, adding 1.0mL of 5% phenol solution, shaking up, quickly dropwise adding 5.0mL of concentrated sulfuric acid, developing for 20min at room temperature, correcting zero point by blank, detecting the light absorption value at 490nm, substituting into a glucose standard curve, and calculating the concentration of polysaccharide in the sample, which is shown in Table 4, Table 5, Table 6, Table 7, Table 8 and Table 9.

TABLE 4

TABLE 5

TABLE 6

TABLE 7

TABLE 8

TABLE 9

According to the experimental data analysis of the examples 1-3, the contents of the flavone, saponin and polysaccharide in the traditional Chinese medicine composition in the optimal weight part are greatly improved relative to the other important compositions in weight part after fermentation when the medicinal components are the same. The motherwort, the astragalus and the angelica in the traditional Chinese medicine compound can enrich the blood and promote the blood circulation, and after feeding tests, the egg yield and the eggshell quality of the chicken are improved; the prepared rehmannia root, the folium artemisiae argyi and the glossy privet fruit have the effects of nourishing yin and supplementing blood, reducing the damage rate of chicken flocks and increasing the number of normal eggs, so that the traditional Chinese medicine formula has an obvious effect.

According to the experimental data analysis of the comparative examples 1-3, the content of the effective components of the fermented traditional Chinese medicine composition is obviously improved compared with that of the traditional Chinese medicine composition which is not fermented, and the mixed fermentation strain used for fermenting the traditional Chinese medicine composition inhibits the growth of harmful bacteria under the synergistic effect of the strain, deepens the fermentation degree of the composition, largely destroys the cell walls of the traditional Chinese medicine, and improves the contents of polysaccharide, saponin and flavone, so compared with the comparative example 3, the strain is preferred by the formula, and the combined synergistic effect can enable the fermentation effect of the traditional Chinese medicine composition to be more obvious.

Application example

The application method comprises the following steps:

experimental groups: weighing the fermented traditional Chinese medicine composition used in the test, adding the fermented traditional Chinese medicine composition into the complete feed according to the mass fraction of 0.3%, and feeding the laying hens all day long.

Control group: the fermented traditional Chinese medicine composition is not added, and the complete feed is only fed.

Examples 1, 2, 3 and comparative examples 1, 2, 3 were tested and the data analyzed under the same feeding and testing conditions, respectively, and statistics are as follows:

index detection: egg weight, egg production, breakage rate, eggshell color and quality, see table 10.

The detection method comprises the following steps:

(1) egg weight: weighing method, randomly taking 360 eggs, placing in a carton, placing on an electronic platform scale, and weighing.

(2) Laying rate: the egg laying amount in the same day is divided by the total number of laying hens in the henhouse.

(3) Breakage rate: the number of broken eggs divided by the number of eggs laid on the day.

(4) Color of eggshell: the egg shell pigment mainly comprises protoporphyrin IX, biliverdin and biliverdin zinc chelate, and the three components form different colors according to different proportions. The color of the eggshell is measured by a QCR eggshell color measuring instrument, pure black is 0, pure white is x, correction is carried out, and the smaller the data is, the darker the color of the eggshell is

(5) The quality of the eggshell is as follows: the microelement is used as enzyme prosthetic group, and can influence the formation and change of crystal, structure of eggshell crystal, and eggshell strength by using eggshell strength tester, and the international requirement that the egg can bear 2.7-3.6 Kg/cm when placed vertically²And (4) pressure.

Watch 10

As can be seen from the experimental data in Table 10, the Chinese medicinal composition related to the invention effectively improves the production performance of laying hens, improves the laying rate and enhances the quality of eggs, and the preparation method is simple and easy to implement, and is particularly more convenient for large-scale poultry farming.

The experiment shows that the traditional Chinese medicine is used for preventing and treating salpingitis of the laying hens, improving the production performance of the laying hens, improving the laying rate and enhancing the quality of eggs, the method is simple and easy to implement, the feeding is really available, and the method is particularly convenient for large-scale poultry farming. The test is based on a classic formula, through a method of defense-qi-nutrient-blood syndrome differentiation and six-channel syndrome differentiation, combined with clinical manifestations of intestinal diseases of poultry and egg laying diseases of laying hens, a prevention and treatment scheme is continuously screened and optimized, and finally a traditional Chinese medicine for treating the diseases of the laying hens, adjusting the balance of intestinal flora, remarkably improving the egg laying rate of the laying hens, improving the quality of eggs, enhancing the immune function of the laying hens, preventing and treating salpingitis of the laying hens, improving the disease resistance of the laying hens and reducing the death rate is obtained; meanwhile, the traditional Chinese medicine composition can prevent and control intestinal inflammation caused by bacterial invasion and infection of the intestinal tract, and a set of successful prevention and treatment technology is formed.

The fermented traditional Chinese medicine composition prepared in example 1 has 4 kinds of medicines different from the fermented traditional Chinese medicine composition prepared in comparative example, that is, the fermented traditional Chinese medicine composition prepared in example 1 contains motherwort herb, Chinese angelica, astragalus root and acanthopanax root which are different from the fermented traditional Chinese medicine composition prepared in comparative example 1 which does not contain the four medicines. Experiments prove that the fermented traditional Chinese medicine composition prepared in the example 1 has a remarkably better effect than the fermented traditional Chinese medicine composition prepared in the comparative example 1. In the formula, the motherwort herb is bitter and pungent, is slightly cold, enters liver, heart and bladder channels, has the effects of activating blood circulation to dissipate blood stasis, inducing diuresis to alleviate edema, and clearing heat and removing toxicity, and is a monarch drug. The angelica sinensis can enrich and activate blood, and promote blood circulation to stop pain; radix astragali has effects of invigorating qi, invigorating yang, invigorating qi, and consolidating superficial resistance; the acanthopanax senticosus has the effects of tonifying qi, strengthening spleen and tonifying kidney, and the 4 medicines are used as ministerial medicines. The compatibility of the prescription is characterized in that tonics are added into the blood-activating and stasis-removing medicines, so that the aim of expelling evil factors and strengthening body resistance is achieved, and the prescription is one of the main principles of Chinese veterinarians for treating diseases. This is also the principle that the prevention and treatment effect of the fermented traditional Chinese medicine composition of example 1 is significantly better than that of the fermented traditional Chinese medicine composition of comparative example 1.

The feeding effect of the fermented traditional Chinese medicine composition prepared in the example 1 and the fermented traditional Chinese medicine composition prepared in the comparative example is obviously better than that of the fermented traditional Chinese medicine composition prepared in the comparative example 1, the comparative example 2 and the comparative example 3.

The traditional Chinese medicine used by the invention has the characteristics of wide sources, low price, small toxic and side effects, no residue, safety, convenience, no drug resistance and the like.

The traditional Chinese medicine composition can effectively prevent and treat laying hen salpingitis, and laying hen diseases seriously hindering the sustainable development of poultry industry comprise oophoritis, salpingitis and peritonitis, which are common diseases of chickens, particularly laying hens, and are clinically characterized by ovary, oviduct and peritonitis, and follicle deformation, congestion and bleeding are serious, and the etiology of the disease is complex, so that the disease is one of the frequently encountered diseases threatening the healthy development of the poultry industry.

Claims

1. A fermented traditional Chinese medicine composition for preventing and treating salpingitis of laying hens and improving the production performance of the laying hens is characterized by comprising the following raw material components:

and (3) auxiliary material part: rice chaff, bean pulp, corn and stone powder;

strain part: lactic acid bacteria and Bacillus subtilis.

2. The fermented traditional Chinese medicine composition of claim 1, wherein the traditional Chinese medicine composition comprises the following raw material components in parts by weight:

the traditional Chinese medicine part: 190 parts of motherwort 160-;

3. The fermented traditional Chinese medicine composition of claim 2, wherein the traditional Chinese medicine composition comprises the following raw material components in parts by weight:

4. the fermented traditional Chinese medicine composition of claim 3, wherein the enterococcus faecalis is strain number CICC23658, the Lactobacillus acidophilus strain number CICC22162, and the Bacillus subtilis strain number CICC 20872.

5. The method for preparing the fermented traditional Chinese medicine composition according to any one of claims 1 to 4, which is characterized by comprising the following steps:

(1) preparing lactobacillus liquid and bacillus subtilis liquid;

6. The preparation method according to claim 5, wherein the pulverizing mesh of the Chinese medicinal material part and the adjuvant part in step (2) is 40 mesh.

7. The method according to claim 5, wherein the solid fermentation material of the step (2) has a water content of 40 to 42%.

8. The process according to claim 5, wherein the solid fermentation material in the step (3) is subjected to solid fermentation in a sealed bag under conditions of 20 to 25 ℃ for a fermentation period of 18 to 25 days.

9. Use of the fermented traditional Chinese medicine composition of any one of claims 1 to 4 or the fermented traditional Chinese medicine composition prepared by the method of any one of claims 5 to 8 for feeding poultry.

10. The use of claim 9, wherein the fermented traditional Chinese medicine composition is used in raising chickens.