CN113617225A - Cleaning device and method for exogenous viruses in animal serum - Google Patents
Cleaning device and method for exogenous viruses in animal serum Download PDFInfo
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- CN113617225A CN113617225A CN202110822178.4A CN202110822178A CN113617225A CN 113617225 A CN113617225 A CN 113617225A CN 202110822178 A CN202110822178 A CN 202110822178A CN 113617225 A CN113617225 A CN 113617225A
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- 210000002966 serum Anatomy 0.000 title claims abstract description 71
- 238000004140 cleaning Methods 0.000 title claims abstract description 70
- 241000700605 Viruses Species 0.000 title claims abstract description 36
- 241001465754 Metazoa Species 0.000 title claims abstract description 34
- 238000000034 method Methods 0.000 title claims description 8
- 238000001914 filtration Methods 0.000 claims abstract description 52
- 239000012528 membrane Substances 0.000 claims abstract description 40
- 238000007789 sealing Methods 0.000 claims abstract description 25
- 230000000694 effects Effects 0.000 claims abstract description 15
- 230000007246 mechanism Effects 0.000 claims description 34
- 239000000243 solution Substances 0.000 claims description 10
- 108090000190 Thrombin Proteins 0.000 claims description 6
- 239000000706 filtrate Substances 0.000 claims description 6
- 238000002156 mixing Methods 0.000 claims description 6
- 238000004806 packaging method and process Methods 0.000 claims description 6
- 229960004072 thrombin Drugs 0.000 claims description 6
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 claims description 3
- 229940037003 alum Drugs 0.000 claims description 3
- 230000029087 digestion Effects 0.000 claims description 3
- 230000008014 freezing Effects 0.000 claims description 3
- 238000007710 freezing Methods 0.000 claims description 3
- 239000000463 material Substances 0.000 claims description 3
- 239000011259 mixed solution Substances 0.000 claims description 3
- 238000002360 preparation method Methods 0.000 claims description 3
- 230000009471 action Effects 0.000 claims description 2
- 239000007788 liquid Substances 0.000 claims 3
- 230000010405 clearance mechanism Effects 0.000 description 8
- 238000003825 pressing Methods 0.000 description 7
- 239000012982 microporous membrane Substances 0.000 description 5
- 230000001147 anti-toxic effect Effects 0.000 description 3
- 238000009434 installation Methods 0.000 description 3
- 238000011045 prefiltration Methods 0.000 description 3
- 239000000047 product Substances 0.000 description 3
- 239000002033 PVDF binder Substances 0.000 description 2
- 230000008859 change Effects 0.000 description 2
- 239000002657 fibrous material Substances 0.000 description 2
- -1 polypropylene Polymers 0.000 description 2
- 229920002981 polyvinylidene fluoride Polymers 0.000 description 2
- 239000011148 porous material Substances 0.000 description 2
- 239000004743 Polypropylene Substances 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 230000003247 decreasing effect Effects 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
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- 229920005594 polymer fiber Polymers 0.000 description 1
- 229920001155 polypropylene Polymers 0.000 description 1
- 229920001343 polytetrafluoroethylene Polymers 0.000 description 1
- 239000004810 polytetrafluoroethylene Substances 0.000 description 1
- 230000008569 process Effects 0.000 description 1
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- 238000003908 quality control method Methods 0.000 description 1
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D61/00—Processes of separation using semi-permeable membranes, e.g. dialysis, osmosis or ultrafiltration; Apparatus, accessories or auxiliary operations specially adapted therefor
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
- A61K35/14—Blood; Artificial blood
- A61K35/16—Blood plasma; Blood serum
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2/00—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor
- A61L2/0005—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor for pharmaceuticals, biologicals or living parts
- A61L2/0011—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor for pharmaceuticals, biologicals or living parts using physical methods
- A61L2/0017—Filtration
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2202/00—Aspects relating to methods or apparatus for disinfecting or sterilising materials or objects
- A61L2202/20—Targets to be treated
- A61L2202/22—Blood or products thereof
Abstract
The invention relates to a cleaning device for exogenous viruses in animal serum, which comprises a bottom plate frame, support columns, a base frame, a cleaning assembly and a sealing frame, wherein the support columns are uniformly arranged at the upper end of the bottom plate frame, the base frame is arranged between the upper ends of the support columns, the cleaning assembly is arranged at the upper end of the base frame through a screw, and the sealing frame is arranged at the upper end of the cleaning assembly through a screw. The invention can solve the problems that the existing equipment has poor filtering effect, can not effectively protect the filtering membrane, causes the phenomenon that the filtering membrane is easy to deform when being pressed and causes the phenomenon that the filtering membrane is easy to damage when the virus is filtered, and simultaneously, the existing equipment has poor mounting and dismounting effects when the virus is filtered, can not timely clean the surface of the filtering membrane, can not adjust the number of the filtering membranes according to the use requirement, influences the filtering effect of serum and the like.
Description
Technical Field
The invention relates to the technical field of biology, in particular to a device and a method for cleaning exogenous viruses in animal serum.
Background
For antitoxin and antiserum biological products derived from animal serum, due to the extremely complicated purification process and difficult quality control, virus removal and inactivation are very important in the production process of antitoxin and antiserum taking animal blood as a raw material, and the safety of the antitoxin and antiserum biological products can be ensured only in this way.
At present, the existing animal serum exogenous viruses generally have the following defects in the process of cleaning: 1. when the existing equipment is used for filtering and cleaning viruses, the filtering effect is poor, the filtering is insufficient, the filtering membrane cannot be effectively protected, the phenomenon that the filtering membrane is easy to deform when being pressed is caused, and the phenomenon that the filtering membrane is easy to damage is caused; 2. when the existing equipment is used for filtering and cleaning viruses, the mounting and dismounting effects of the filtering membrane are poor, the surface of the filtering membrane cannot be cleaned in time, the number of the filtering membrane cannot be adjusted according to the use requirement, and the filtering effect of serum is influenced.
Disclosure of Invention
In order to solve the above problems, the present invention provides a device for cleaning exogenous viruses in animal serum, which can solve the problems mentioned in the background art.
In order to achieve the purpose, the invention adopts the following technical scheme to realize the purpose: the utility model provides a cleaning device of animal serum exogenous virus, includes bottom plate frame, support column, base frame, clearance subassembly and sealed frame, bottom plate frame upper end evenly install the support column, install the base frame between the support column upper end, there is the clearance subassembly base frame upper end through the mounting screw, there is sealed frame clearance subassembly upper end through the mounting screw.
The clearance subassembly include casing frame, link, mounting bracket, locating rack, locking mechanism and clearance mechanism, casing frame be cylindrical structure, the casing frame outside evenly is provided with the open slot, the link is installed to casing frame inside symmetry, the link is the cruciform structure, install the mounting bracket between the link, all be provided with the spout on mounting bracket and the casing frame, install the locating rack between the spout, the inside locking mechanism that is provided with of locating rack, locking mechanism is fixed with the spout chucking, install clearance mechanism in the open slot, clearance mechanism middle part is connected with the locating rack, clearance mechanism is inboard hugs closely mutually with the mounting bracket.
Clearance mechanism including clearance frame, handle frame, connecting plate and filtration frame, clearance frame be the arc structure, clearance frame and open slot cooperate, evenly be provided with the mounting hole on the clearance frame, clearance frame outside mid-mounting has the handle frame, evenly installs the connecting plate on the clearance frame concave surface, the connecting plate position is adjustable, the connecting plate mid-mounting has the filtration frame.
The filter frame comprises two bearing plates and two filter membranes, wherein the two bearing plates are connected through screws.
Further, the base frame be the rectangle structure, the base frame is connected with the clearance subassembly through the screw, the fluid-discharge tube is installed to the base frame lower extreme.
Further, seal carrier upper end be the arc structure, the seal carrier is connected with the clearance subassembly through the screw, the seal carrier upper end is provided with the feed liquor pipe, is provided with the manometer on the feed liquor pipe, the manometer can accurate demonstration clearance subassembly inside pressure size.
Furthermore, the cross-section of the mounting frame is of a cross structure, sealing gaskets are symmetrically arranged on the outer side surface of the mounting frame, and the sealing gaskets are made of wear-resistant rubber materials.
Furthermore, the cross section of the positioning frame is of an I-shaped structure, the middle of the positioning frame is provided with a locking mechanism, the outer side of the positioning frame is symmetrically provided with clamping grooves, and sealing strips are uniformly arranged in the clamping grooves.
Further, locking mechanism including adjusting pole, briquetting, wedge, guide bar, expanding spring and pressure strip, locating rack middle part be connected with the regulation pole through screw-thread fit's mode, adjust the pole outside and be provided with the screw, adjust the pole inboard and install the briquetting, the locating rack is inside to be provided with logical groove, it is provided with the guide frame to lead to inslot symmetry, is connected with the guide bar through sliding fit's mode in the guide frame, the wedge is installed to the guide bar inboard, the wedge is hugged closely with the briquetting, is provided with expanding spring on the guide bar, the pressure strip is installed in the guide bar outside.
Further, the pressure strip be protruding type structure, the pressure strip cooperatees with the spout, evenly is equipped with the non-skid protrusions on the pressure strip lateral surface, the non-skid protrusions is regular wave structure.
Furthermore, the bearing plate is of a fan-shaped structure, arc-shaped grooves are evenly formed in the outer side face of the bearing plate, and round holes are evenly formed in the arc-shaped grooves.
Furthermore, the filtering membrane is a biological permeable membrane, the outer side of the filtering membrane is tightly attached to the inner side face of the bearing plate, and filtering holes are uniformly formed in the filtering membrane.
In addition, the invention also provides a cleaning method of the cleaning device for the animal serum exogenous viruses, which comprises the following steps:
s1, plasma filtration: balancing the frozen animal plasma to room temperature, filtering the plasma by medical gauze, and collecting the filtered filtrate;
s2, addition of thrombin: uniformly mixing the thrombin solution and the calcium chloride solution according to a certain proportion for 28-32 minutes, and then adding the mixed solution into the filtrate obtained in the step S1;
s3, mixed digestion: mixing and digesting the reacted plasma in the step S2 at normal temperature for 150 minutes, and freezing and storing the digested plasma;
s4, serum preparation: balancing the plasma in the step S3 to room temperature again, centrifuging the plasma by a centrifuge, and filtering the centrifuged solution to prepare animal serum;
s5, serum regulation: adjusting the pH value of the serum in the step S4 to 7.5-8.0, adding a certain amount of alum, and standing for 50-150 minutes;
s6, serum filtration: injecting the serum in the step S5 into the cleaning assembly through the sealing frame, and gradually flowing the serum downwards from the interior of the filter frame under the action of certain pressure, wherein the filter frame in the cleaning mechanism can effectively clean the virus in the serum, and the filter frames can be adjusted in different quantities according to the use requirements to ensure the filtering effect of the serum;
s7, collecting and packaging: and bottling and collecting the filtered serum in the step S6, and sealing and packaging the collected serum solution.
The invention has the beneficial effects that:
1. according to the cleaning device for the exogenous viruses in the animal serum, the cleaning assembly can effectively filter and clean the viruses in the serum, the filtering effect is good, the cleaning is complete, and the safety in use of serum biological products is ensured.
2. According to the cleaning device for the animal serum exogenous viruses, the locking mechanism can effectively lock and fix the positioning frame, so that the positioning frame is convenient to mount and dismount, the positioning frame can be increased or decreased according to the use requirement of the filtering membrane, and the viruses in the serum are fully filtered.
3. According to the cleaning device for the exogenous viruses in the animal serum, the filter frame is designed in a sandwich manner, and when the filter membrane is under pressure, the filter membrane is separated from the filter frame. The supporting plate can protect the filter membrane effectively, prevents that the filter membrane pressurized from taking place the phenomenon of deformation, avoids the filter membrane to take place to damage, can avoid the filtration pore diameter on the filter membrane to change simultaneously, guarantees the filtration clearance effect of serum.
Drawings
The invention is further illustrated with reference to the following figures and examples.
FIG. 1 is a schematic perspective view of the present invention;
FIG. 2 is a schematic perspective view of the present invention;
FIG. 3 is a schematic cross-sectional view of FIG. 2 in accordance with the present invention;
FIG. 4 is a schematic perspective view of the cleaning assembly of the present invention;
FIG. 5 is a schematic view of the internal structure of the cleaning assembly of the present invention;
FIG. 6 is a schematic cross-sectional view of the plate frame, the support posts and the cleaning assembly of the present invention;
FIG. 7 is a schematic cross-sectional view of the spacer and locking mechanism of the present invention;
FIG. 8 is a schematic view of a first cross-sectional configuration of the cleaning mechanism of the present invention;
FIG. 9 is a schematic view of a second cross-sectional configuration of the cleaning mechanism of the present invention;
FIG. 10 is an enlarged schematic view of the invention at A in FIG. 8;
FIG. 11 is a flow chart of the operational steps of the present invention.
Detailed Description
In order to make the technical problems, technical solutions and advantageous effects to be solved by the present invention more clearly apparent, the present invention is further described in detail below with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention.
As shown in fig. 1 to 11, a cleaning device for foreign viruses in animal serum comprises a base plate frame 1, a support column 2, a base plate frame 3, a cleaning assembly 4 and a sealing frame 5, and is characterized in that: the utility model discloses a cleaning assembly, including base frame 1, base frame 3, clearance subassembly 4 upper end has a sealed frame 5 through the mounting screw, the base frame 2 is evenly installed to 1 upper end of base frame, install base frame 3 between 2 upper ends of support column, 3 upper ends of base frame have the clearance subassembly 4 through the mounting screw, the clearance subassembly 4 upper end has a sealed frame 5 through the mounting screw.
5 upper ends of seal frame be the arc structure, seal frame 5 is connected with clearance subassembly 4 through the screw, 5 upper ends of seal frame are provided with the feed liquor pipe, are provided with the manometer on the feed liquor pipe, concrete during operation, seal frame 5 and clearance subassembly 4 sealing connection prevent the phenomenon of taking place to leak, the manometer can accurately show pressure, prevent that the too big diaphragm that leads to of pressure from taking place the phenomenon of deformation.
The cleaning assembly 4 comprises a shell frame 41, a connecting frame 42, a mounting frame 43, a positioning frame 44, a locking mechanism 45 and a cleaning mechanism 46, wherein the shell frame 41 is of a cylindrical structure, open grooves are uniformly formed in the outer side of the shell frame 41, the connecting frame 42 is symmetrically mounted in the shell frame 41, the connecting frame 42 is of a cross structure, the mounting frame 43 is mounted between the connecting frames 42, sliding grooves are formed in the mounting frame 43 and the shell frame 41, the positioning frame 44 is mounted between the sliding grooves, the locking mechanism 45 is arranged in the positioning frame 44, the locking mechanism 45 is fixedly clamped with the sliding grooves, the cleaning mechanism 46 is mounted in the open grooves, the middle of the cleaning mechanism 46 is connected with the positioning frame 44, the inner side of the cleaning mechanism 46 is tightly attached to the mounting frame 43, during specific work, animal serum is manually injected into the shell frame 41 through the sealing frame 5, the cleaning mechanism 46 can clean and filter viruses in the animal serum, the connection can be dismantled to clearance mechanism 46, the clearance of the inside diaphragm of clearance mechanism 46 of being convenient for, and locating rack 44 can increase and decrease according to the quantity of diaphragm, and locking mechanism 45 can fix locating rack 44, and the installation and the dismantlement of locating rack 44 of being convenient for, and locating rack 44 can support and seal clearance mechanism 46 lateral wall simultaneously, guarantees the filter effect of serum.
The cross-section of the positioning frame 44 is of an I-shaped structure, the locking mechanism 45 is arranged in the middle of the positioning frame 44, the clamping grooves are symmetrically formed in the outer side of the positioning frame 44, the sealing strips are uniformly arranged in the clamping grooves, the sealing strips can effectively play a role in sealing, and gaps are prevented from appearing between the positioning frame 44 and the filter frame 464.
The locking mechanism 45 comprises an adjusting rod 451, a pressing block 452, a wedge-shaped block 453, a guide rod 454, a telescopic spring 455 and a pressing plate 456, the middle of the positioning frame 44 is connected with the adjusting rod 451 in a threaded fit manner, screw holes are formed in the outer side of the adjusting rod 451, a pressing block 452 is installed on the inner side of the adjusting rod 451, a through groove is formed in the positioning frame 44, guide frames are symmetrically arranged in the through groove, the guide frame is connected with the guide rod 454 in a sliding fit manner, the wedge-shaped block 453 is installed on the inner side of the guide rod 454, the wedge-shaped block 453 is tightly attached to the pressing block 452, the telescopic spring 455 is arranged on the guide rod 454, the pressing plate 456 is installed on the outer side of the guide rod 454, when the positioning frame 44 is installed in specific work, when the positioning frame 44 is installed, the positioning frame 44 is manually placed between the through the sliding groove in the housing frame 41, the adjusting rod 451 is rotated by a wrench, the adjusting rod 451 drives the pressing block 452 to move inwards, the pressing block 452 to move outwards by the wedge-shaped block 453, the pressure strip 456 can be tightly attached to the inside of the sliding groove, so that the positioning frame 44 can be fixed, and meanwhile, the operation is simple and convenient, and the installation and the disassembly of the positioning frame 44 are facilitated.
The pressure strip 456 is a convex structure, the pressure strip 456 is matched with the sliding groove, and the outer side surface of the pressure strip 456 is uniformly provided with the anti-skid protrusions.
The filter frame 464 comprises support plates 4641 and filter membranes 4642, the number of the support plates 4641 is two, the filter membranes 4642 are arranged between the support plates 4641, and the two support plates 4641 are connected by screws, so that the support plates 4641 and the filter membranes 4642 are designed in a sandwich manner, and in specific work, the support plates 4641 can effectively support the filter membranes 4642, and the filter membranes 4642 are prevented from deforming when pressure is too high, so that the filter pore size of the filter membranes 4642 is changed, and the filter effect of serum is influenced.
The supporting plate 4641 is of a fan-shaped structure, arc-shaped grooves are uniformly formed in the outer side surface of the supporting plate 4641, and circular holes are uniformly formed in the arc-shaped grooves.
The filtering membrane 4642 is a biological permeable membrane, the outer side of the filtering membrane 4642 is tightly attached to the inner side surface of the supporting plate 4641, and the supporting plate 4641 can effectively prevent the filtering membrane 4642 from deforming and prevent the filtering membrane 4642 from deforming under stress.
The filtering membrane 4642 is composed of a prefiltration microporous membrane and a virus-removing microporous membrane compounded on the surface of the prefiltration microporous membrane, the prefiltration microporous membrane is made of porous fiber materials and polyvinylidene fluoride, the porous fiber materials are selected from glass fibers, polypropylene fibers or polymer fibers, and the virus-removing microporous membrane is made of polyvinylidene fluoride or polytetrafluoroethylene.
In addition, the invention also provides a cleaning method of the cleaning device for the animal serum exogenous viruses, which comprises the following steps:
s1, plasma filtration: balancing the frozen animal plasma to room temperature, filtering the plasma by medical gauze, and collecting the filtered filtrate;
s2, addition of thrombin: uniformly mixing the thrombin solution and the calcium chloride solution according to a certain proportion for 28-32 minutes, and then adding the mixed solution into the filtrate obtained in the step S1;
s3, mixed digestion: mixing and digesting the reacted plasma in the step S2 at normal temperature for 150 minutes, and freezing and storing the digested plasma;
s4, serum preparation: balancing the plasma in the step S3 to room temperature again, centrifuging the plasma by a centrifuge, and filtering the centrifuged solution to prepare animal serum;
s5, serum regulation: adjusting the pH value of the serum in the step S4 to 7.5-8.0, adding a certain amount of alum, and standing for 50-150 minutes;
s6, serum filtration: injecting the serum in the step S5 into the cleaning assembly 4 through the sealing frame 5, wherein the serum gradually flows out from the inside of the filter frame 464 under a certain pressure, the filter frame 464 inside the cleaning mechanism 46 can effectively clean the virus inside the serum, and the filter frame 464 can adjust different amounts according to the use requirement to ensure the filtering effect of the serum;
s7, collecting and packaging: and bottling and collecting the filtered serum in the step S6, and sealing and packaging the collected serum solution.
The foregoing illustrates and describes the principles, general features, and advantages of the present invention. It will be understood by those skilled in the art that the present invention is not limited to the embodiments described above, which are given by way of illustration of the principles of the present invention, and that various changes and modifications may be made without departing from the spirit and scope of the invention as defined by the appended claims. The scope of the invention is defined by the appended claims and equivalents thereof.
Claims (10)
1. The utility model provides a cleaning device of animal serum exogenous virus, includes bottom plate frame (1), support column (2), base frame (3), clearance subassembly (4) and seal frame (5), its characterized in that: the upper end of the base plate frame (1) is uniformly provided with supporting columns (2), a base frame (3) is arranged between the upper ends of the supporting columns (2), the upper end of the base frame (3) is provided with a cleaning component (4) through a screw, and the upper end of the cleaning component (4) is provided with a sealing frame (5) through a screw;
the cleaning component (4) comprises a shell frame (41), a connecting frame (42), a mounting frame (43), a positioning frame (44), a locking mechanism (45) and a cleaning mechanism (46), the shell frame (41) is of a cylindrical structure, open slots are uniformly formed in the outer side of the shell frame (41), connecting frames (42) are symmetrically arranged in the shell frame (41), the connecting frames (42) are of a cross structure, mounting frames (43) are arranged between the connecting frames (42), sliding grooves are formed in the mounting frames (43) and the shell frame (41), positioning frames (44) are arranged between the sliding grooves, locking mechanisms (45) are arranged in the positioning frames (44), the locking mechanisms (45) are fixedly clamped with the sliding grooves, cleaning mechanisms (46) are arranged in the open slots, the middle parts of the cleaning mechanisms (46) are connected with the positioning frames (44), and the inner sides of the cleaning mechanisms (46) are tightly attached to the mounting frames (43);
the cleaning mechanism (46) comprises a cleaning frame (461), a handle frame (462), a connecting plate (463) and a filtering frame (464), wherein the cleaning frame (461) is of an arc-shaped structure, the cleaning frame (461) is matched with an open slot, mounting holes are uniformly formed in the cleaning frame (461), the handle frame (462) is mounted in the middle of the outer side of the cleaning frame (461), the connecting plate (463) is uniformly mounted on the inner concave surface of the cleaning frame (461), the position of the connecting plate (463) is adjustable, and the filtering frame (464) is mounted in the middle of the connecting plate (463);
the filter frame (464) comprises two support plates (4641) and two filter membranes (4642), wherein the filter membranes (4642) are arranged between the support plates (4641), and the two support plates (4641) are connected through screws.
2. The device for cleaning foreign viruses in animal serum, according to claim 1, wherein: the base frame (3) is of a rectangular structure, the base frame (3) is connected with the cleaning assembly (4) through screws, and a liquid discharge pipe is installed at the lower end of the base frame (3).
3. The device for cleaning foreign viruses in animal serum, according to claim 1, wherein: the upper end of the sealing frame (5) is of an arc-shaped structure, the sealing frame (5) is connected with the cleaning assembly (4) through a screw, a liquid inlet pipe is arranged at the upper end of the sealing frame (5), and a pressure gauge is arranged on the liquid inlet pipe.
4. The device for cleaning foreign viruses in animal serum, according to claim 1, wherein: the cross-section of the mounting frame (43) is of a cross structure, sealing gaskets are symmetrically arranged on the outer side surface of the mounting frame (43), and the sealing gaskets are made of wear-resistant rubber materials.
5. The device for cleaning foreign viruses in animal serum, according to claim 1, wherein: the cross section of the positioning frame (44) is of an I-shaped structure, a locking mechanism (45) is arranged in the middle of the positioning frame (44), clamping grooves are symmetrically formed in the outer side of the positioning frame (44), and sealing strips are uniformly arranged in the clamping grooves.
6. The device for cleaning foreign viruses in animal serum, according to claim 1, wherein: locking mechanism (45) including adjusting pole (451), briquetting (452), wedge (453), guide bar (454), expanding spring (455) and pressure strip (456), locating rack (44) middle part be connected with through screw-thread fit's mode and adjust pole (451), it is provided with the screw to adjust pole (451) outside, adjust pole (451) inboard and install briquetting (452), locating rack (44) inside is provided with logical groove, the logical inslot symmetry is provided with the guide frame, be connected with guide bar (454) through sliding fit's mode in the guide frame, wedge (453) are installed to guide bar (454) inboard, wedge (453) are hugged closely with briquetting (452), be provided with expanding spring (455) on guide bar (454), pressure strip (456) are installed in guide bar (454) outside.
7. The device for cleaning foreign viruses in animal serum according to claim 6, wherein: the compacting plate (456) is of a convex structure, the compacting plate (456) is matched with the sliding groove, and anti-skid protrusions are uniformly arranged on the outer side surface of the compacting plate (456).
8. The device for cleaning foreign viruses in animal serum, according to claim 1, wherein: the bearing plate (4641) is of a fan-shaped structure, arc-shaped grooves are uniformly formed in the outer side surface of the bearing plate (4641), and circular holes are uniformly formed in the arc-shaped grooves.
9. The device for cleaning foreign viruses in animal serum, according to claim 1, wherein: the filtering membrane (4642) is a biological permeable membrane, and the outer side of the filtering membrane (4642) is tightly attached to the inner side surface of the supporting plate (4641).
10. The device for cleaning the foreign viruses in the serum of the animals according to any one of claims 1 to 9, wherein: the method also comprises a method for cleaning the cleaning device of the animal serum exogenous viruses, and specifically comprises the following steps:
s1, plasma filtration: balancing the frozen animal plasma to room temperature, filtering the plasma by medical gauze, and collecting the filtered filtrate;
s2, addition of thrombin: uniformly mixing the thrombin solution and the calcium chloride solution according to a certain proportion for 28-32 minutes, and then adding the mixed solution into the filtrate obtained in the step S1;
s3, mixed digestion: mixing and digesting the reacted plasma in the step S2 at normal temperature for 150 minutes, and freezing and storing the digested plasma;
s4, serum preparation: balancing the plasma in the step S3 to room temperature again, centrifuging the plasma by a centrifuge, and filtering the centrifuged solution to prepare animal serum;
s5, serum regulation: adjusting the pH value of the serum in the step S4 to 7.5-8.0, adding a certain amount of alum, and standing for 50-150 minutes;
s6, serum filtration: injecting the serum in the step S5 into the cleaning assembly (4) through the sealing frame (5), wherein the serum gradually flows downwards from the inside of the filter frame (464) under the action of certain pressure, the filter frame (464) in the cleaning mechanism (46) can effectively clean the virus in the serum, and the filter frame (464) can be adjusted to different quantities according to the use requirement to ensure the filtering effect of the serum;
s7, collecting and packaging: and bottling and collecting the filtered serum in the step S6, and sealing and packaging the collected serum solution.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110822178.4A CN113617225B (en) | 2021-07-21 | 2021-07-21 | Device and method for cleaning exogenous viruses in animal serum |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110822178.4A CN113617225B (en) | 2021-07-21 | 2021-07-21 | Device and method for cleaning exogenous viruses in animal serum |
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| Publication Number | Publication Date |
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| CN113617225A true CN113617225A (en) | 2021-11-09 |
| CN113617225B CN113617225B (en) | 2024-04-02 |
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| CN202110822178.4A Active CN113617225B (en) | 2021-07-21 | 2021-07-21 | Device and method for cleaning exogenous viruses in animal serum |
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Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
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2021
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| JPH06292827A (en) * | 1993-02-11 | 1994-10-21 | Seikagaku Kogyo Co Ltd | Filter and consisting of material which prevents passing of virus and filtering method using the same |
| EP0798003A2 (en) * | 1996-03-21 | 1997-10-01 | Bayer Corporation | Use of structured depth filters for virus removal |
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| CN212758055U (en) * | 2020-06-01 | 2021-03-23 | 杭州科百特过滤器材有限公司 | Filter for removing viruses and filtering system comprising same |
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|---|---|
| CN113617225B (en) | 2024-04-02 |
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