CN113615776B - Lactic acid bacteria compound microecological preparation for improving poultry adenogastric development and application thereof - Google Patents
Lactic acid bacteria compound microecological preparation for improving poultry adenogastric development and application thereof Download PDFInfo
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- CN113615776B CN113615776B CN202110724297.6A CN202110724297A CN113615776B CN 113615776 B CN113615776 B CN 113615776B CN 202110724297 A CN202110724297 A CN 202110724297A CN 113615776 B CN113615776 B CN 113615776B
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Classifications
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/70—Feeding-stuffs specially adapted for particular animals for birds
- A23K50/75—Feeding-stuffs specially adapted for particular animals for birds for poultry
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/16—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
- A23K10/18—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions of live microorganisms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/163—Sugars; Polysaccharides
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
- A23V2400/165—Paracasei
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
- A23V2400/173—Reuteri
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/51—Bifidobacterium
- A23V2400/515—Animalis
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Abstract
The invention provides a lactobacillus compound microecological preparation for improving the development of the adenosis stomach of poultry and application thereof. The lactobacillus compound microecological preparation comprises living bacteria with content not less than 2.00×10 8 CFU/g lactobacillus reuteri powder with viable bacteria content not less than 5.00×10 7 CFU/g animal bifidobacterium powder with viable bacteria content not less than 5.00×10 7 CFU/g lactobacillus paracasei powder and carrier with content not less than 500 mg/g. The lactobacillus composite microecological preparation can obviously reduce the adenogastric index of the broiler chickens, reduce the ratio of adenogastric to myogastric, improve the integrity of the adenogastric nipple and mucous membrane, effectively improve the development of the adenogastric of the broiler chickens, has the advantages of safety, no residue, no side effect and the like, and has wide application value.
Description
Technical Field
The invention mainly relates to the technical field of microorganisms and feed additives, in particular to a lactobacillus compound microecological preparation for improving the development of the adenosis of poultry and application thereof.
Background
The adenosis acts as an important digestive organ in chickens and plays an important role in the dietary digestion of chickens. There are two glands in the glandular gastric mucosa: the superficial layer is a single tubular gland, secreting mucus; the deep layer is a multiple tubular gland and mainly has the functions of secreting hydrochloric acid and pepsin. The damage of the adenosis stomach possibly leads to hypersecretion of hydrochloric acid, leads to hypersecretion of hydrochloric acid to influence the pH value of the adenosis stomach and the myostomach, further influences digestion, and corrodes the adenosis gastric mucosa and the myogastric keratoma to cause erosion of the adenosis gastric mucosa and the myogastric keratoma; swelling and blockage of the adenogastric catheter can cause the secretion of hydrochloric acid and pepsin which cannot be secreted, thereby reducing digestive juice and further causing worsening of adenogastric inflammation; swelling of the adenoma stomach and erosion of the glandular gastric mucosa and myogastric cuticle can lead to eating difficulties and severe decline of the digestive ability of chickens. Meanwhile, intestinal flora which normally grows under the regulation of gastric acid is disturbed due to the reduction of gastric acid secretion, and harmful bacteria are caused to reproduce, so that enteritis, diarrhea and other diseases are caused, and the production performance and the cultivation benefit of poultry animals are greatly reduced.
Lactic acid bacteria are probiotics widely existing in human and livestock intestinal tracts, and are applied due to the advantages of safety, greenness, no pollution, no residue and the like. Lactic acid bacteria have different promotion effects on the aspects of microecological balance of livestock and poultry gastrointestinal tracts, growth and development of gastrointestinal tracts, repair of gastrointestinal mucous membranes and the like. However, lactic acid bacteria are various, the single lactic acid bacteria have weak effect, and some biological bacterial agents prepared by lactic acid bacteria have the defects of high price, too complex or single bacterial strain compounding, low viable bacteria amount and the like, or the compound bacterial strains have no synergistic effect, even antagonism is generated, so that the effect is influenced, the use effect is reduced, and the use cost is increased. Therefore, lactic acid bacteria agents with excellent effects and simple components are still one of the research hotspots in the market at present.
Disclosure of Invention
In order to overcome the defects and shortcomings in the prior art, the invention provides the lactobacillus composite microecological preparation for improving the development of the stomach of the poultry and the application thereof.
In order to achieve the aim, the invention is realized by the following technical scheme:
the invention provides a lactobacillus compound microecological preparation for improving the development of the adenoma stomach of poultry, which is characterized in that: the lactobacillus composite microecological preparation comprises lactobacillus reuteri powder, bifidobacterium animalis powder, lactobacillus paracasei powder and a carrier.
Further, the live bacteria content of the lactobacillus reuteri powder is not less than 2.00 multiplied by 10 8 CFU/g; the live bacteria content of the animal bifidobacterium powder is not less than 5.00 multiplied by 10 7 CFU/g; the live bacteria content of the Lactobacillus paracasei powder is not less than 5.00×10 7 CFU/g; the content of the carrier is not less than 500 mg/g.
Further, the mass ratio of the lactobacillus reuteri bacteria powder to the bifidobacterium animalis bacteria powder to the lactobacillus paracasei bacteria powder is 1-5:1-5:1-5.
Preferably, the mass ratio of the lactobacillus reuteri powder to the bifidobacterium animalis powder to the lactobacillus paracasei powder is 4:1:1.
Further, the lactobacillus reuteri bacterial powder is prepared from the following components in percentage by weight with the preservation number of CCTCC NO: the lactobacillus reuteri NSL0501 of M2021628 is obtained by culturing, fermenting, centrifuging and vacuum freeze-drying.
Further, the nucleotide sequence of the lactobacillus reuteri is shown as SEQ ID No. 1.
Furthermore, the bifidobacterium animalis powder adopts a preservation number of CCTCC NO: the bifidobacterium animalis NSY0201 of M2021629 is obtained by culturing, fermenting, centrifuging and vacuum freeze-drying.
Further, the nucleotide sequence of the bifidobacterium animalis is shown as SEQ ID No. 2.
Further, the lactobacillus paracasei bacterial powder adopts a preservation number of CCTCC NO: lactobacillus paracasei NSL0201 of M2021626 is obtained by culturing, fermenting, centrifuging and vacuum freeze-drying.
Further, the nucleotide sequence of the lactobacillus paracasei is shown as SEQ ID No. 3.
Further, the carrier is maltodextrin, and the 80-mesh oversize material is 0.
The invention also provides application of the lactobacillus composite microecological preparation in preparing a feed additive for poultry cultivation.
Furthermore, when in use, the lactobacillus compound microecological preparation is mixed into the complete compound feed according to the mass of 2-5 per mill of the total weight of the complete compound feed, and after being stirred uniformly, the compound feed is fed to poultry according to the normal feeding standard.
Furthermore, the lactobacillus compound microecological preparation can obviously reduce the adenogastric index and the adenogastric and myogastric ratio of poultry and improve the integrity of the adenogastric nipple and mucous membrane, thereby effectively improving the problem of the dysplasia of the adenogastric of the poultry caused by the complete compound pellet feed.
Further, the poultry is broiler chicken.
The invention also provides application of the lactobacillus composite microecological preparation in preparing medicines or nutritional products for preventing and treating inflammatory diseases of poultry caused by adenosis and gastric dysplasia or injury.
Compared with the prior art, the invention has the following advantages and beneficial technical effects:
1. the lactobacillus reuteri NSL0501 selected by the invention is selected from food milk fans, has good safety, can effectively relieve proventriculitis swelling, reduce inflammatory infiltration and repair gastrointestinal mucosal tissues.
2. The bifidobacterium animalis NSY0201 and lactobacillus paracasei NSL0201 sieve selected by the invention are selected from natural fermented cheeses, have good safety, can rapidly grow and reproduce in gastrointestinal tracts, effectively inhibit the reproduction of pathogenic bacteria and reduce the occurrence of adeno-gastric inflammation by efficiently secreting metabolic products such as lactic acid and the like.
3. The lactobacillus composite microecological preparation is prepared by mixing the bacterial powder prepared by fermenting and freeze-drying the three strains with a carrier, has simple and safe components, can be used for broiler chicken cultivation, has the effects of obviously reducing the adenogastric index, reducing the adenogastric and myogastric ratio, improving the integrity of the adenogastric nipple and mucous membrane, and can effectively improve the development of the adenogastric of broilers chicken, and the synergistic effect of the three strains has a certain repairing effect on ulcers caused by the dysplasia of the adenogastric, can damage gastric mucous membrane, reduce the occurrence of gastric inflammation, further prevent diseases such as proventriculitis and the like, and finally improve the production performance of broilers and the income of farmers.
Drawings
Fig. 1 shows the effect of the lactobacillus complex microecological preparation on the stomach papilla and mucous membrane of broiler chickens, wherein A is a group I control group and B is a group II experimental group.
Detailed Description
The technical scheme of the invention is further described in detail by combining the following specific examples. In the following examples, unless otherwise specified, all experimental methods used are conventional and all materials, reagents, etc. are commercially available from biological or chemical reagent companies.
Example 1 screening and identification of strains
1. Screening and identification of lactobacillus reuteri NSL0501
1. The traditional fermented milk fan from the herdsman in Ganzi Tibetan, autonomous state of Sichuan province is taken as a sample. Grinding the sample in a sterilized mortar, diluting with sterile water to give 10 ml sample solution, and mixing with sterile water according to 10 -3 -10 -4 After dilution in the ratio of (2), 200. Mu.l of each of the obtained solutions was applied to a modified MRS fixed medium, and the resulting mixture was allowed to stand at 37℃for 16 hours. Picking single colony on the plate, inoculating the single colony into improved MRS liquid culture medium, standing at 36deg.C for 16h, and repeatedly streaking the cultured bacterial liquid into improved MRS solid by streaking methodOn the plate, purification was performed, and the purification was repeated 3 times. After three generations of purification, the single colony number obtained was NSL0501.
Bacterial colony of strain NSL0501 on the modified MRS fixing plate is milky white to yellowish, is round, has a diameter of 1-3 mm, has smooth and matt surface, is convex in the middle, is opaque, has neat edges and has no halo.
The single colony is selected and inoculated in an improved MRS liquid culture medium, the culture is carried out at 36 ℃ for 16h, then bacterial liquid is centrifuged at 4 ℃ and 12,000 rpm, bacterial mud is taken and mixed with 30% glycerol at a ratio of 1:1 and then stored in a refrigerator at-70 ℃ for subsequent experiments.
2. Identification of 16S rRNA of Strain
DNA of the extracted strain NSL0501 is used as a template, bacterial 16S rRNA universal primers 27F and 1492R are used for amplification, amplified fragments are sent to a biological company for sequencing, and a measuring result is shown as SEQ ID No. 1. BLAST comparison results show that the strain NSL0501 and the strain NSL0501Limosilactobacillus reuteriThe sequence similarity of (2) was 99.60%, and thus it was judged that the strain NSL0501 was Lactobacillus reuteri.
And (3) performing strain preservation on the screened strain NSL0501, wherein the preservation unit of the lactobacillus reuteri NSL0501 is as follows: china Center for Type Culture Collection (CCTCC); address: chinese university of Wuhan; preservation date: 2021, 5, 31; lactobacillus reuteriLimosilactobacillus reuteriThe preservation number of the steel is CCTCC NO: m2021628.
2. Screening and identification of bifidobacterium animalis NSY0201
1. A natural fermented cheese derived from the inner mongolian tin Lin Guole allium was used as a sample. 3 samples are taken and added into 10% skim milk culture medium according to the dosage of 1%, and are cultured at 37 ℃, and after the samples are coagulated, gradient dilution is carried out, and the samples are gradually diluted to 10-8 gradient. Selecting 3 proper dilutions, respectively taking 1mL of the strain in an improved MRS solid culture medium, carrying out anaerobic culture for 48 hours at 37 ℃, carrying out colony morphology observation, selecting single colonies with different morphologies, carrying out streak separation on the improved MRS solid culture medium again, carrying out anaerobic culture for 48 hours at 37 ℃, repeating the streak separation for 3 generations to obtain purified strains, carrying out gram staining and microscopic observation, selecting out gram staining positive strains, and carrying out microscopic observation on the strains which are spherical, rod-shaped or multi-shaped, wherein the strain liquid of one strain cannot form firm precipitation after centrifugation, the bacterial mud is loose, and the capsule staining of the strain is thickest after negative staining by a negative staining method. And then the content of the extracellular polysaccharide of the bacterium is measured by a phenol-sulfuric acid method and a colorimetric method, and the result shows that the extracellular polysaccharide yield of the bacterium is as high as 306 mg/L and the bacterium is numbered NSY0201.
Observing morphological characteristics of a strain NSY0201, wherein the strain NSY0201 is gram positive, and bacterial colonies are milky to yellowish, circular or quasi-circular and have diameters of 1-2mm; the surface is raised, smooth and matt, and the edge has irregular wave shape; the thallus is short rod-shaped, both ends are rounded, forked is visible, the surface is smooth, and no spore exists.
2. Identification of 16S rRNA of Strain
DNA of the strain NSY0201 is extracted as a template, the bacterial 16S rRNA universal primers 27F and 1492R are utilized for amplification, and amplified fragments are sent to a biological company for sequencing, wherein the sequencing result is shown as SEQ ID No. 2. BLAST comparison shows that strain NSY0201 andBifidobacterium animalisthe sequence similarity of (2) was 99.79%, and thus it was judged that the strain NSY0201 was bifidobacterium animalis.
Performing strain preservation on the screened strain NSY0201, wherein the bifidobacterium animalis NSY collection unit of 0201: china Center for Type Culture Collection (CCTCC); address: chinese university of Wuhan; preservation date: 2021, 5, 31; bifidobacterium animalisBifidobacterium animalisThe preservation number of the steel is CCTCC NO: m2021629.
3. Screening and identification of Lactobacillus paracasei NSL0201
1. Taking traditional fermented yoghourt from the tryptic Sonchi area of the Tibetan autonomous region as a sample, uniformly mixing the sample, absorbing 1mL of the sample, adding the sample into a sterile test tube containing 9mL of 0.9% physiological saline, taking 200 mu L of sample liquid, coating the sample liquid on an improved MRS solid culture medium, culturing the sample liquid at 37 ℃ for 48 hours, picking a typical single colony of a bacterial strain, inoculating the typical single colony on the improved MRS solid culture medium again for streak purification culture, picking the single colony, transferring the single colony into the improved MRS liquid culture medium for amplification culture, repeatedly streaking the cultured bacterial liquid on the improved MRS solid plate by a streak method after culturing at 37 ℃, purifying the bacterial liquid repeatedly for 3 times. After three generations of purification, single colony number was obtained as NSL0201.
Strain NSL0201 is a facultative anaerobic bacterium, gram positive, and the colony is milky white, round or quasi-round, with a diameter above 1.0 mm; the surface is raised smoothly and the edge is neat; the thalli are short-rod-shaped, the two ends of the thalli are round, are arranged in a short chain shape, have smoother surfaces and are free of spores.
2. Identification of 16S rRNA of Strain
DNA of the strain NSL0201 is extracted as a template, the bacterial 16S rRNA universal primers 27F and 1492R are utilized for amplification, and amplified fragments are sent to a biological company for sequencing, wherein the sequencing result is shown as SEQ ID No. 3. BLAST comparison results show that the strain NSL0201 and the strain NSL0201Lacticaseibacillus paracaseiThe sequence similarity of (2) was 99.50%, and thus it was judged that the strain NSL0501 was Lactobacillus paracasei.
Performing strain preservation on the screened strain NSL0201, wherein the preservation unit of the lactobacillus paracasei NSL0201 is as follows: china Center for Type Culture Collection (CCTCC); address: chinese university of Wuhan; preservation date: 2021, 5, 31; lactobacillus paracaseiLactobacillus paracaseiThe preservation number of the steel is CCTCC NO: m2021626.
Example 2 preparation of lactic acid bacteria Compound microecological preparation
The lactobacillus composite microecological preparation provided by the invention comprises the following components: the viable bacteria content is not less than 2.00×10 8 CFU/g lactobacillus reuteri NSL0501, viable bacteria content of not less than 5.00×10 7 CFU/g bifidobacterium animalis NSY0201 with viable bacteria content of not less than 5.00×10 7 CFU/g lactobacillus paracasei NSL0201, carrier maltodextrin content is not less than 500 mg/g.
Marking the three strains on an improved MRS solid plate, culturing for 48 hours at 37 ℃, then picking single bacterial colony, inoculating the single bacterial colony into an improved MRS liquid culture medium, and culturing for 16h at 37 ℃; inoculating the bacterial liquid into an improved MRS liquid culture medium of 2L according to the inoculation amount of 3%, and culturing at 37 ℃ for 16h until the bacterial liquid is in a logarithmic phase; centrifuging the bacterial suspension at 4deg.C for 10 min at 12000r/min, discarding supernatant, and collecting thallus; washing the thalli with ultrapure water for 3 times, and centrifuging to obtain bacterial sludge; the bacterial mud is resuspended by 12% skim milk solution, and three bacterial strains bacterial powders are obtained after vacuum freeze drying and freeze drying.
Mixing lactobacillus reuteri NSL0501 bacterial powder, bifidobacterium animalis NSY0201 bacterial powder and lactobacillus paracasei NSL0201 in a mass ratio of 4:1:1, and then mixing with carrier maltodextrin with a content of not less than 500 mg/g to finally prepare the lactobacillus composite microecological preparation.
Example 3 application of lactic acid bacteria composite microecological preparation in broiler chicken raising
The lactobacillus compound microecological preparation is added into the complete compound feed according to the mass of 2-5 per mill of the total weight of the complete compound feed.
Randomly dividing 400 Ross 308 broilers which are healthy at 1 day old and have insignificant weight difference into 2 treatment groups, and feeding complete compound pellet feed; wherein the group I is a control group, and no microecological additive is added into the feed; group II is an experimental group, wherein 3 per mill of lactobacillus compound microecological preparation (3 kg of lactobacillus compound microecological preparation is added into each ton of complete feed) is added into the feed; each treatment group was set with 5 replicates of 40 chickens per replicate group.
The chicken house, the chicken coop and the surrounding environment are disinfected before the test, constant illumination and mechanical ventilation are kept in the test process, the test adopts a cage culture mode, and the chicken house adopts an automatic temperature control heating device. The test broiler is free to feed and drink water, and the test period is 42 days.
At the end of the trial, 10 broilers of average body weight were slaughtered for each repetition, the adenogastric index, the adenogastric and myogastric ratio were calculated, and the tissue integrity of the adenogastric nipple, mucous membrane, etc. was observed. The calculation formula of the adenogastric index and the ratio of the adenogastric to myogastric is as follows:
adenogastric index (%) = (adenogastric weight/broiler weight) x 100%;
adenogastric and myogastric ratio (%) = (adenogastric weight/myogastric weight) ×100%.
The results are shown in Table 1 and FIG. 1.
Table 1 influence of the complex microecological formulation on the stomach of broiler chickens
| Group of | Adenosis and gastric weight (g) | Myogastric weight (g) | Living body weight (g) | Adenogastric index (%) | Adenosis-gastrosis ratio (%) |
| Group I | 9.65±1.08 a | 27.92±2.55 a | 2615±63 a | 0.37±0.03 a | 32.8±1.02 a |
| Group II | 7.32±0.91 b | 35.22±2.08 b | 2905±79 b | 0.25±0.03 b | 22.7±1.16 b |
Note that: the same row of superscript lower case letters differ significantly (P < 0.05).
Feeding the complete compound pellet feed can cause the increase of the stomach index, the stomach and the myogastric ratio of the broiler chickens, thereby affecting the stomach development and the stomach digestion function. As can be seen from Table 1, in the whole 42-day broiler breeding process, the weight, the index and the ratio of the stomach to the stomach of the group II broiler are obviously lower than those of the group I, and the weight and the living weight of the stomach are obviously higher than those of the group I, so that the lactobacillus composite microecological preparation disclosed by the invention can effectively improve the stomach development of the broiler, reduce the adverse effect of the complete compound pellet feed on the stomach of the broiler, promote the development of the stomach digestion function, improve the growth performance of the broiler, increase the weight of the broiler and finally increase the breeding income.
As can be seen from fig. 1, the group I broiler (fig. 1A) had a swollen adenostomach, different nipple sizes, irregular arrangement, and partial bleeding points; the group II broiler (figure 1B) had intact adenogastric mucosa, no swelling, uniform nipple size, and regular arrangement, and no bleeding point. Therefore, the lactobacillus composite microecological preparation has remarkable effect on repairing the stomach nipple and mucous membrane of the broiler chickens, and improves the inflammatory symptoms of the adenogastric swelling and bleeding points, so that the lactobacillus composite microecological preparation is very suitable for preparing and preventing the inflammatory diseases of the broiler chickens caused by adenogastric dysplasia or injury.
The above embodiments are only for illustrating the technical solution of the present invention, and are not limiting; although the invention has been described in detail with reference to the foregoing embodiments, it will be apparent to one skilled in the art that modifications may be made to the technical solutions described in the foregoing embodiments, or equivalents may be substituted for some of the technical features thereof; such modifications and substitutions do not depart from the spirit and scope of the corresponding technical solutions.
Sequence listing
<110> Shandong Yi Hao Biotech Co., ltd
<120> a lactobacillus compound microecological preparation for improving the development of the adenogastric of poultry and application thereof
<160> 3
<170> SIPOSequenceListing 1.0
<210> 1
<211> 1506
<212> DNA
<213> Lactobacillus reuteri (Limosilactobacillus reuteri)
<400> 1
ggggcctgcg ctataatgca gtcgtacgca ctggcccaac tgattgatgg tgcttgcacc 60
tgattgacga tggatcacca gtgagtggcg gacgggtgag taacacgtag gtaacctgcc 120
ccggagcggg ggataacatt tggaaacaga tgctaatacc gcataacaac aaaagccgca 180
tggcttttgt ttgaaagatg gctttggcta tcactctggg atggacctgc ggtgcattag 240
ctagttggta aggtaacggc ttaccaaggc gatgatgcat agccgagttg agagactgat 300
cggccacaat ggaactgaga cacggtccat actcctacgg gaggcagcag tagggaatct 360
tccacaatgg gcgcaagcct gatggagcaa caccgcgtga gtgaagaagg gtttcggctc 420
gtaaagctct gttgttggag aagaacgtgc gtgagagtaa ctgttcacgc agtgacggta 480
tccaaccaga aagtcacggc taactacgtg ccagcagccg cggtaatacg taggtggcaa 540
gcgttatccg gatttattgg gcgtaaagcg agcgcaggcg gttgcttagg tctgatgtga 600
aagccttcgg cttaaccgaa gaagtgcatc ggaaaccggg cgacttgagt gcagaagagg 660
acagtggaac tccatgtgta gcggtggaat gcgtagatat atggaagaac accagtggcg 720
aaggcggctg tctggtctgc aactgacgct gaggctcgaa agcatgggta gcgaacagga 780
ttagataccc tggtagtcca tgccgtaaac gatgagtgct aggtgttgga gggtttccgc 840
ccttcagtgc cggagctaac gcattaagca ctccgcctgg ggagtacgac cgcaaggttg 900
aaactcaaag gaattgacgg gggcccgcac aagcggtgga gcatgtggtt taattcgaag 960
ctacgcgaag aaccttacca ggtcttgaca tcttgcgcta accttagaga taaggcgttc 1020
ccttcgggga cgcaatgaca ggtggtgcat ggtcgtcgtc agctcgtgtc gtgagatgtt 1080
gggttaagtc ccgcaacgag cgcaaccctt gttactagtt gccagcattg agttgggcac 1140
tctagtgaga ctgccggtga caaaccggag gaaggtgggg acgacgtcag atcatcatgc 1200
cccttatgac ctgggctaca cacgtgctac aatggacggt acaacgagtc gcaaactcgc 1260
gagagtaagc taatctctta aagccgttct cagttcggac tgtaggctgc aactcgccta 1320
cacgaagtcg gaatcgctag taatcgcgga tcagcatgcc gcggtgaata cgttcccggg 1380
ccttgtacac accgcccgtc acaccatggg agtttgtaac gcccaaagtc ggtggcctaa 1440
cctttatgga gggagccgcc taaggcggga cagatgactg ggggaagtcg aacaagattg 1500
ccccct 1506
<210> 2
<211> 1462
<212> DNA
<213> bifidobacterium animalis (Bifidobacterium animalis)
<400> 2
gatgaacgct ggcggcgtgc ttaacacatg caagtcgaac gggatccctg gcagcttgct 60
gtcggggtga gagtggcgaa cgggtgagta atgcgtgacc aacctgccct gtgcaccgga 120
atagctcctg gaaacgggtg gtaataccgg atgctccgct ccatcgcatg gtggggtggg 180
aaatgctttt gcggcatggg atggggtcgc gtcctatcag cttgttggcg gggtgatggc 240
ccaccaaggc gttgacgggt agccggcctg aragggtgac cggccacatt gggactgaga 300
tacggcccag actcctacgg gaggcagcag tggggaatat tgcacaatgg gcgcaagcct 360
gatgcagcga cgccgcgtgc gggatggagg ccttcgggtt gtaaaccgct tttgttcaag 420
ggcaaggcac ggtttcggcc gtgttgagtg gattgttcga ataagcaccg gctaactacg 480
tgccagcagc cgcggtaata cgtagggtgc gagcgttatc cggatttatt gggcgtaaag 540
ggctcgtagg cggttcgtcg cgtccggtgt gaaagtccat cgcctaacgg tggatctgcg 600
ccgggtacgg gcgggctgga gtgcggtagg ggagactgga attcccggtg taacgtgtgg 660
aatgtgtaga tatcgggaag aacaccaatg gcgaaggcag gtctctgggc ccgtcactga 720
cgctgaggag cgaaagcgtg aggagcgaac aggattagat accctggtag tccacgccgt 780
aaacggtgga tgctggatgt ggggcccttt ccacgggtcc cgtgtcggag ccaacgcgtt 840
aagcatcccg cctggggagt acggccgcaa ggctaaaact caaagaaatt gacgggggcc 900
cgcacaagcg gcggagcatg cggattaatt cgatgcaacg cgaagaacct tacctgggct 960
tgacatgtgc cggatcgccg tggagacacg gtttcccttc ggggccggtt cacaggtggt 1020
gcatggtcgt cgtcagctcg tgtcgtgaga tgttgggtta agtcccgcaa cgagcgcaac 1080
cctcgccgca tgttgccagc gggtgatgcc gggaactcat gtgggaccgc cggggtcaac 1140
tcggaggaag gtggggatga cgtcagatca tcatgcccct tacgtccagg gcttcacgca 1200
tgctacaatg gccggtacaa cgcggtgcga cacggtgacg tggggcggat cgctgaaaac 1260
cggtctcagt tcggatcgca gtctgcaact cgactgcgtg aaggcggagt cgctagtaat 1320
cgcggatcag caacgccgcg gtgaatgcgt tcccgggcct tgtacacacc gcccgtcaag 1380
tcatgaaagt gggtagcacc cggagccggt ggcccgaccc ttgtgggggg agccgtctaa 1440
ggtgagactc gtgattggga ct 1462
<210> 3
<211> 1455
<212> DNA
<213> Lactobacillus paracasei (Lactobacillus paracasei)
<400> 3
gatcctgtct tcttagtttg atctggctcc tcttgcttgc acccacatga tcatgtgaac 60
gagtggcgga cgggtgagta acacgtgggt aacctgccct taagtggggg ataacatttg 120
gaaacagatg ctaataccgc atagatccaa gaaccgcatg gttcttggct gaaagatggc 180
gtaagctatc gcttttggat ggacccgcgg cgtattagct agttggtgag gtaatggctc 240
accaaggcga tgatacgtag ccgaactgag aggttgatcg gccacattgg gactgagaca 300
cggcccaaac tcctacggga ggcagcagta gggaatcttc cacaatggac gcaagtctga 360
tggagcaacg ccgcgtgagt gaagaaggct ttcgggtcgt aaaactctgt tgttggagaa 420
gaatggtcgg cagagtaact gttgtcggcg tgacggtatc caaccagaaa gccacggcta 480
actacgtgcc agcagccgcg gtaatacgta ggtggcaagc gttatccgga tttattgggc 540
gtaaagcgag cgcaggcggt tttttaagtc tgatgtgaaa gccctcggct taaccgagga 600
agcgcatcgg aaactgggaa acttgagtgc agaagaggac agtggaactc catgtgtagc 660
ggtgaaatgc gtagatatat ggaagaacac cagtggcgaa ggcggctgtc tggtctgtaa 720
ctgacgctga ggctcgaaag catgggtagc gaacaggatt agataccctg gtagtccatg 780
ccgtaaacga tgaatgctag gtgttggagg gtttccgccc ttcagtgccg cagctaacgc 840
attaagcatt ccgcctgggg agtacgaccg caaggttgaa actcaaagga attgacgggg 900
gcccgcacaa gcggtggagc atgtggttta attcgaagca acgcgaagaa ccttaccagg 960
tcttgacatc ttttgatcac ctgagagatc aggtttcccc ttcgggggca aaatgacagg 1020
tggtgcatgg ttgtcgtcag ctcgtgtcgt gagatgttgg gttaagtccc gcaacgagcg 1080
caacccttat gactagttgc cagcatttag ttgggcactc tagtaagact gccggtgaca 1140
aaccggagga aggtggggat gacgtcaaat catcatgccc cttatgacct gggctacaca 1200
cgtgctacaa tggatggtac aacgagttgc gagaccgcga ggtcaagcta atctcttaaa 1260
gccattctca gttcggactg taggctgcaa ctcgcctaca cgaagtcgga atcgctagta 1320
atcgcggatc agcacgccgc ggtgaatacg ttcccgggcc ttgtacacac cgcccgtcac 1380
accatgagag tttgtaacac ccgaagccgg tggcgtaccc cttttaggga ccccccccta 1440
taaggtggcc acctg 1455
Claims (5)
1. A lactobacillus compound microecological preparation for improving the development of the adenoma stomach of poultry is characterized in that: the lactobacillus composite microecological preparation is prepared from lactobacillus reuteri powder, bifidobacterium animalis powder, lactobacillus paracasei powder and a carrier;
the live bacteria content of the lactobacillus reuteri powder is not less than 2.00 multiplied by 10 8 CFU/g; the live bacteria content of the animal bifidobacterium powder is not less than 5.00 multiplied by 10 7 CFU/g; the live bacteria content of the Lactobacillus paracasei powder is not less than 5.00×10 7 CFU/g; the content of the carrier is not less than 500 mg/g; the mass ratio of the lactobacillus reuteri powder to the bifidobacterium animalis powder to the lactobacillus paracasei powder is 1-5:1-5:1-5;
the lactobacillus reuteri bacterial powder is prepared from the following components in percentage by weight with the preservation number of CCTCC NO: the lactobacillus reuteri NSL0501 of M2021628 is obtained by culturing, fermenting, centrifuging and vacuum freeze-drying;
the bifidobacterium animalis powder adopts a preservation number of CCTCC NO: the bifidobacterium animalis NSY0201 of M2021629 is obtained by culturing, fermenting, centrifuging and vacuum freeze-drying;
the lactobacillus paracasei bacterial powder adopts a preservation number of CCTCC NO: lactobacillus paracasei NSL0201 of M2021626 is obtained by culturing, fermenting, centrifuging and vacuum freeze-drying.
2. Use of the lactic acid bacteria complex microecological preparation according to claim 1 for the preparation of a feed additive for poultry farming.
3. The use according to claim 2, characterized in that: when in use, the lactobacillus compound microecological preparation is mixed into the complete compound feed according to the mass of 2 to 5 per mill of the total weight of the complete compound feed, and after being stirred uniformly, the compound feed is fed to poultry according to the normal feeding standard.
4. A use according to claim 3, characterized in that: the lactobacillus composite microecological preparation can obviously reduce the adenogastric index and the adenogastric and myogastric ratio of the poultry and improve the integrity of the adenogastric nipple and mucous membrane, thereby effectively solving the problem of dysplasia of the adenogastric of the poultry caused by complete compound pellet feed.
5. Use of the lactic acid bacteria complex microecological preparation according to claim 1 for the preparation of a medicament or a nutritional product for the prevention and treatment of inflammatory diseases of poultry caused by adenomatosis or lesions.
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| CN101946850A (en) * | 2009-12-11 | 2011-01-19 | 中国农业科学院饲料研究所 | Lactobacillus reuteri fermented liquid feed, preparation method and application thereof |
| CN105707507A (en) * | 2016-03-17 | 2016-06-29 | 青岛根源生物技术集团有限公司 | Lactic acid bacteria compound micro-ecological preparation and application thereof |
| CN112674232A (en) * | 2020-12-29 | 2021-04-20 | 喀什泰昆饲料有限责任公司 | Feed for improving muscular stomach development of yellow-feathered broilers and preparation method and application thereof |
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| CN101946850A (en) * | 2009-12-11 | 2011-01-19 | 中国农业科学院饲料研究所 | Lactobacillus reuteri fermented liquid feed, preparation method and application thereof |
| CN105707507A (en) * | 2016-03-17 | 2016-06-29 | 青岛根源生物技术集团有限公司 | Lactic acid bacteria compound micro-ecological preparation and application thereof |
| CN112674232A (en) * | 2020-12-29 | 2021-04-20 | 喀什泰昆饲料有限责任公司 | Feed for improving muscular stomach development of yellow-feathered broilers and preparation method and application thereof |
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