CN113615536A - Formula and preparation process of culture medium for symbiosis of fungi and plants - Google Patents

Formula and preparation process of culture medium for symbiosis of fungi and plants Download PDF

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CN113615536A
CN113615536A CN202111089610.XA CN202111089610A CN113615536A CN 113615536 A CN113615536 A CN 113615536A CN 202111089610 A CN202111089610 A CN 202111089610A CN 113615536 A CN113615536 A CN 113615536A
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water
sulfate
fungi
urea
plants
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郑殿峰
冯乃杰
牟保民
冯胜杰
刘玲
黄喜心
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Guangdong Ocean University
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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
    • A01G24/10—Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
    • A01G24/10—Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material
    • A01G24/12—Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material containing soil minerals
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
    • A01G24/20—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material
    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05B—PHOSPHATIC FERTILISERS
    • C05B17/00—Other phosphatic fertilisers, e.g. soft rock phosphates, bone meal
    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G5/00—Fertilisers characterised by their form
    • C05G5/40—Fertilisers incorporated into a matrix
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Environmental Sciences (AREA)
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  • Biotechnology (AREA)
  • Pest Control & Pesticides (AREA)
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  • Tropical Medicine & Parasitology (AREA)
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  • Soil Sciences (AREA)
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  • General Engineering & Computer Science (AREA)
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  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

The invention discloses a culture medium for symbiosis of fungi and plants, which comprises the following components in parts by weight: 1-100 g of phosphoric acid, 1-100 g of potassium sulfate, 1-100 g of zinc sulfate, 1-100 g of urea, 1-100 g of calcium lignosulphonate, 1-100 g of sodium chloride, 1-100 g of ammonia water, 1-100 g of 7-water magnesium sulfate, 1-100 g of 7-water ferrous sulfate, 1-100 g of glucose, 10-400 g of lignite, 10-400 g of medical stone, 10-400 g of zeolite powder, 10-400 g of phosphogypsum, 10-400 g of humus soil and 10-400 g of diatomite. According to the preparation process of the culture medium for the symbiosis of the fungi and the plants, urea can be decomposed into ammonium bicarbonate to absorb roots of the plants after being applied to soil through soil microorganisms, meanwhile, the corresponding nutrient components can be supplemented by the internal glucose components during symbiotic culture, and meanwhile, a positive and negative rotation alternative rotation mode is adopted in the preparation process, so that the situation that the multiple nutrient components are mixed and stirred by rotating in the same direction is avoided, and the uniformity of the whole culture medium during the mixing of the multiple components is improved, and the mixing preparation time is shortened.

Description

Formula and preparation process of culture medium for symbiosis of fungi and plants
Technical Field
The invention relates to the technical field related to fungus culture, in particular to a culture medium formula for symbiosis of fungi and plants and a preparation process thereof.
Background
With the development of society, the agricultural level of people is also continuously improved, the symbiosis phenomenon between fungi and plant roots in soil often exists in agriculture, the symbiosis between the fungi and the plant roots can effectively enlarge the absorption surface of the root system, meanwhile, the symbiotic fungi can absorb certain organic matters such as saccharides and the like from host plants so as to supplement nutrition for the symbiotic fungi, and can also absorb specific nutrients and water in the soil to supply the nutrients to plants, however, the corresponding culture medium is usually used for effectively adding required nutrients to the fungi and the plants in the symbiotic culture process of the fungi and the plants.
However, most of the existing culture medium formulas and preparation processes for symbiosis of fungi and plants have the following problems:
the existing culture medium for fungus and plant symbiosis has poor symbiotic culture effect on fungi and plants in the use process, is inconvenient to supply corresponding supplementary effective nutrient components, is long in the whole preparation time in the process of preparing the culture medium for fungus and plant symbiosis, and is inconvenient to uniformly mix various nutrient components, so that the uniformity of the whole components in the mixing process is greatly reduced.
Therefore, we propose a culture medium formula for symbiosis of fungi and plants and a preparation process thereof, so as to solve the problems proposed in the above.
Disclosure of Invention
The invention aims to provide a formula and a preparation process of a culture medium for symbiosis of fungi and plants, and aims to solve the problems that the existing culture medium for symbiosis of fungi and plants in the market has poor symbiotic culture effect on the fungi and the plants in the using process, is inconvenient to provide corresponding supplementary effective nutrient components, has long overall preparation time in the preparation process of the culture medium for symbiosis of the fungi and the plants, is inconvenient to uniformly mix various nutrient components, and greatly reduces the uniformity of the whole components in mixing.
In order to achieve the purpose, the invention provides the following technical scheme: a culture medium for symbiosis of fungi and plants comprises the following components in parts by weight: 1-100 g of phosphoric acid, 1-100 g of potassium sulfate, 1-100 g of zinc sulfate, 1-100 g of urea, 1-100 g of calcium lignosulphonate, 1-100 g of sodium chloride, 1-100 g of ammonia water, 1-100 g of 7-water magnesium sulfate, 1-100 g of 7-water ferrous sulfate, 1-100 g of glucose, 10-400 g of lignite, 10-400 g of medical stone, 10-400 g of zeolite powder, 10-400 g of phosphogypsum, 10-400 g of humus soil and 10-400 g of diatomite.
Preferably, the culture medium for symbiosis of fungi and plants comprises the following components in parts by weight: 30g of phosphoric acid, 40g of potassium sulfate, 50g of zinc sulfate, 35g of urea, 20g of calcium lignosulphonate, 60g of sodium chloride, 55g of ammonia water, 25g of 7-water magnesium sulfate, 45g of 7-water ferrous sulfate, 80g of glucose, 350g of lignite, 200g of medical stone, 400g of zeolite powder, 250g of phosphogypsum, 150g of humus and 100g of diatomite.
Preferably, the culture medium for symbiosis of fungi and plants comprises the following components in parts by weight: 25g of phosphoric acid, 15g of potassium sulfate, 60g of zinc sulfate, 30g of urea, 40g of calcium lignosulphonate, 90g of sodium chloride, 70g of ammonia water, 60g of 7-water magnesium sulfate, 55g of 7-water ferrous sulfate, 70g of glucose, 300g of lignite, 100g of medical stone, 200g of zeolite powder, 350g of phosphogypsum, 100g of humus and 80g of diatomite.
Preferably, the culture medium for symbiosis of fungi and plants comprises the following components in parts by weight: 70g of phosphoric acid, 80g of potassium sulfate, 35g of zinc sulfate, 10g of urea, 65g of calcium lignosulphonate, 35g of sodium chloride, 25g of ammonia water, 40g of 7-water magnesium sulfate, 35g of 7-water ferrous sulfate, 85g of glucose, 150g of lignite, 300g of medical stone, 100g of zeolite powder, 100g of phosphogypsum, 70g of humus and 30g of diatomite.
Preferably, the culture medium for symbiosis of fungi and plants comprises the following components in parts by weight: 15g of phosphoric acid, 55g of potassium sulfate, 10g of zinc sulfate, 25g of urea, 75g of calcium lignosulphonate, 40g of sodium chloride, 60g of ammonia water, 90g of 7-water magnesium sulfate, 95g of 7-water ferrous sulfate, 35g of glucose, 200g of lignite, 150g of medical stone, 300g of zeolite powder, 200g of phosphogypsum, 250g of humus and 400g of diatomite.
Preferably, the pH value of the fungus and plant symbiotic culture medium obtained by the phosphoric acid, potassium sulfate, zinc sulfate, urea, calcium lignosulphonate, sodium chloride, ammonia water, 7-water magnesium sulfate, 7-water ferrous sulfate, glucose, lignite, medical stone, zeolite powder, phosphogypsum, humus and diatomite is 5.0-7.5.
A preparation process of a culture medium for symbiosis of fungi and plants comprises the following specific steps:
s1, quantitatively and correspondingly adding ammonia water into the lignite, and stirring, airing and grinding the two materials;
s2, adding urea into phosphoric acid to react with each other to obtain urea phosphate which can be used by plants and fungi, stirring, airing and grinding;
s3, mixing and stirring uniformly the product obtained by mixing ammonia water and lignite, the product obtained by mixing urea and phosphoric acid and the rest substances, thereby obtaining the culture substrate for symbiosis of fungi and plants.
Preferably, stirring, airing and grinding three steps are carried out in proper order when aqueous ammonia and brown coal mix, and the stirring mode sets up to just reversing the rotation of alternating.
Preferably, the product obtained by mixing the ammonia water and the lignite, the product obtained by mixing the urea and the phosphoric acid and the rest substances are mixed and stirred in an equal proportion quantitative adding mode, and the mixing and stirring time is 30 min.
A method for using a culture medium for symbiosis of fungi and plants comprises the following specific steps:
s1, taking a certain amount of culture medium for symbiosis of fungi and plants, putting the culture medium into a container such as a bowl and the like, and sterilizing for later use;
s2, soaking the plant seeds in 0.1% potassium permanganate solution for 10 minutes for disinfection, and blotting the plant seeds with sterile paper for later use;
s3, digging a culture medium at the center of the pot on a superclean bench by using a sterile spoon for 4 cm, inoculating fungi to be researched or inspected, covering the medium for 1 cm, sowing the sterilized seeds, and covering the medium for 3 cm;
s4, placing the cultivation pot for inoculation and sowing in an aseptic or ventilated bright environment, pouring sterile water, and culturing while keeping the relative water content at 70-80%.
Compared with the prior art, the invention has the beneficial effects that: the culture medium formula and the preparation process for symbiosis of the fungi and the plants can provide required nutrient components when symbiotic culture of the plants and the fungi is carried out, improve the overall symbiotic culture effect on the fungi and the plants, improve the uniformity when various nutrient components are mixed, and reduce the overall preparation time;
thereby can be after applying into soil through the inside urea of culture medium, the decomposition through soil microorganism forms ammonium bicarbonate, thereby absorb for the root system of plant, utilize inside glucose component can give it to supply corresponding nutrient composition when the symbiotic cultivation of fungi and plant simultaneously, and then improve holistic cultivation effect, the in-process that the culture medium preparation of carrying out the symbiotic fungi and plant adopts the rotatory mode of positive and negative rotation alternating when stirring simultaneously, and then can avoid only can mixing stirring to multiple nutrient composition around same direction, improve whole even degree when multiple component mixes from this, and it is long when reducing holistic mixed preparation.
Drawings
FIG. 1 is a schematic view of the processing flow of the fungus and plant symbiotic culture substrate of the present invention.
Detailed Description
The following will clearly and completely describe the technical solutions in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
The invention provides a technical scheme that: the composite material comprises the following components in parts by weight: 1-100 g of phosphoric acid, 1-100 g of potassium sulfate, 1-100 g of zinc sulfate, 1-100 g of urea, 1-100 g of calcium lignosulphonate, 1-100 g of sodium chloride, 1-100 g of ammonia water, 1-100 g of 7-water magnesium sulfate, 1-100 g of 7-water ferrous sulfate, 1-100 g of glucose, 10-400 g of lignite, 10-400 g of medical stone, 10-400 g of zeolite powder, 10-400 g of phosphogypsum, 10-400 g of humus soil and 10-400 g of diatomite.
Example 1
A culture medium for symbiosis of fungi and plants comprises the following components in parts by weight: 30g of phosphoric acid, 40g of potassium sulfate, 50g of zinc sulfate, 35g of urea, 20g of calcium lignosulphonate, 60g of sodium chloride, 55g of ammonia water, 25g of 7-water magnesium sulfate, 45g of 7-water ferrous sulfate, 80g of glucose, 350g of lignite, 200g of medical stone, 400g of zeolite powder, 250g of phosphogypsum, 150g of humus and 100g of diatomite;
example 2
A culture medium for symbiosis of fungi and plants comprises the following components in parts by weight: 25g of phosphoric acid, 15g of potassium sulfate, 60g of zinc sulfate, 30g of urea, 40g of calcium lignosulphonate, 90g of sodium chloride, 70g of ammonia water, 60g of 7-water magnesium sulfate, 55g of 7-water ferrous sulfate, 70g of glucose, 300g of lignite, 100g of medical stone, 200g of zeolite powder, 350g of phosphogypsum, 100g of humus and 80g of diatomite;
example 3
A culture medium for symbiosis of fungi and plants comprises the following components in parts by weight: 70g of phosphoric acid, 80g of potassium sulfate, 35g of zinc sulfate, 10g of urea, 65g of calcium lignosulphonate, 35g of sodium chloride, 25g of ammonia water, 40g of 7-water magnesium sulfate, 35g of 7-water ferrous sulfate, 85g of glucose, 150g of lignite, 300g of medical stone, 100g of zeolite powder, 100g of phosphogypsum, 70g of humus and 30g of diatomite;
example 4
A culture medium for symbiosis of fungi and plants comprises the following components in parts by weight: 15g of phosphoric acid, 55g of potassium sulfate, 10g of zinc sulfate, 25g of urea, 75g of calcium lignosulphonate, 40g of sodium chloride, 60g of ammonia water, 90g of 7-water magnesium sulfate, 95g of 7-water ferrous sulfate, 35g of glucose, 200g of lignite, 150g of medical stone, 300g of zeolite powder, 200g of phosphogypsum, 250g of humus and 400g of diatomite.
The technical scheme provides a preparation process of a culture medium for symbiosis of fungi and plants, which comprises the following steps:
the method comprises the following specific steps:
s1, quantitatively and correspondingly adding ammonia water into the lignite, and stirring, airing and grinding the two materials;
s2, adding urea into phosphoric acid to react with each other to obtain urea phosphate which can be used by plants and fungi, stirring, airing and grinding;
s3, mixing and stirring uniformly the product obtained by mixing ammonia water and lignite, the product obtained by mixing urea and phosphoric acid and the rest substances, thereby obtaining the culture substrate for symbiosis of fungi and plants.
The three steps of stirring, airing and grinding are sequentially carried out when the ammonia water and the lignite are mixed, and the stirring mode is set to be positive and negative rotation alternating rotation.
And mixing and stirring a product obtained by mixing ammonia water and lignite, a product obtained by mixing urea and phosphoric acid and the rest substances in an equal-proportion quantitative adding mode, wherein the mixing and stirring time is 30 min.
The technical scheme provides a use method of a culture medium for symbiosis of fungi and plants, which comprises the following steps:
the method comprises the following specific steps:
s1, taking a certain amount of culture medium for symbiosis of fungi and plants, putting the culture medium into a container such as a bowl and the like, and sterilizing for later use;
s2, soaking the plant seeds in 0.1% potassium permanganate solution for 10 minutes for disinfection, and blotting the plant seeds with sterile paper for later use;
s3, digging a culture medium at the center of the pot on a superclean bench by using a sterile spoon for 4 cm, inoculating fungi to be researched or inspected, covering the medium for 1 cm, sowing the sterilized seeds, and covering the medium for 3 cm;
s4, placing the cultivation pot for inoculation and sowing in an aseptic or ventilated bright environment, pouring sterile water, and culturing while keeping the relative water content at 70-80%.
Although the present invention has been described in detail with reference to the foregoing embodiments, it will be apparent to those skilled in the art that various changes in the embodiments and/or modifications of the invention can be made, and equivalents and modifications of some features of the invention can be made without departing from the spirit and scope of the invention.

Claims (10)

1. A culture medium for symbiosis of fungi and plants is characterized by comprising the following materials in parts by weight: 1-100 g of phosphoric acid, 1-100 g of potassium sulfate, 1-100 g of zinc sulfate, 1-100 g of urea, 1-100 g of calcium lignosulphonate, 1-100 g of sodium chloride, 1-100 g of ammonia water, 1-100 g of 7-water magnesium sulfate, 1-100 g of 7-water ferrous sulfate, 1-100 g of glucose, 10-400 g of lignite, 10-400 g of medical stone, 10-400 g of zeolite powder, 10-400 g of phosphogypsum, 10-400 g of humus soil and 10-400 g of diatomite.
2. A fungal and plant symbiotic culture substrate according to claim 1, characterized by comprising the following components in parts by weight: 30g of phosphoric acid, 40g of potassium sulfate, 50g of zinc sulfate, 35g of urea, 20g of calcium lignosulphonate, 60g of sodium chloride, 55g of ammonia water, 25g of 7-water magnesium sulfate, 45g of 7-water ferrous sulfate, 80g of glucose, 350g of lignite, 200g of medical stone, 400g of zeolite powder, 250g of phosphogypsum, 150g of humus and 100g of diatomite.
3. A fungal and plant symbiotic culture substrate according to claim 1, characterized by comprising the following components in parts by weight: 25g of phosphoric acid, 15g of potassium sulfate, 60g of zinc sulfate, 30g of urea, 40g of calcium lignosulphonate, 90g of sodium chloride, 70g of ammonia water, 60g of 7-water magnesium sulfate, 55g of 7-water ferrous sulfate, 70g of glucose, 300g of lignite, 100g of medical stone, 200g of zeolite powder, 350g of phosphogypsum, 100g of humus and 80g of diatomite.
4. A fungal and plant symbiotic culture substrate according to claim 1, characterized by comprising the following components in parts by weight: 70g of phosphoric acid, 80g of potassium sulfate, 35g of zinc sulfate, 10g of urea, 65g of calcium lignosulphonate, 35g of sodium chloride, 25g of ammonia water, 40g of 7-water magnesium sulfate, 35g of 7-water ferrous sulfate, 85g of glucose, 150g of lignite, 300g of medical stone, 100g of zeolite powder, 100g of phosphogypsum, 70g of humus and 30g of diatomite.
5. A fungal and plant symbiotic culture substrate according to claim 1, characterized by comprising the following components in parts by weight: 15g of phosphoric acid, 55g of potassium sulfate, 10g of zinc sulfate, 25g of urea, 75g of calcium lignosulphonate, 40g of sodium chloride, 60g of ammonia water, 90g of 7-water magnesium sulfate, 95g of 7-water ferrous sulfate, 35g of glucose, 200g of lignite, 150g of medical stone, 300g of zeolite powder, 200g of phosphogypsum, 250g of humus and 400g of diatomite.
6. A fungal and plant symbiotic culture substrate according to claim 1, characterized in that: the pH value of the fungus and plant symbiotic culture substrate obtained by the phosphoric acid, potassium sulfate, zinc sulfate, urea, calcium lignosulphonate, sodium chloride, ammonia water, 7-water magnesium sulfate, 7-water ferrous sulfate, glucose, lignite, medical stone, zeolite powder, phosphogypsum, humus and diatomite is 5.0-7.5.
7. A process for the preparation of a fungal and plant symbiotic culture substrate according to any one of claims 1 to 6, comprising the following specific steps:
s1, quantitatively and correspondingly adding ammonia water into the lignite, and stirring, airing and grinding the two materials;
s2, adding urea into phosphoric acid to react with each other to obtain urea phosphate which can be used by plants and fungi, stirring, airing and grinding;
s3, mixing and stirring uniformly the product obtained by mixing ammonia water and lignite, the product obtained by mixing urea and phosphoric acid and the rest substances, thereby obtaining the culture substrate for symbiosis of fungi and plants.
8. The process for preparing a fungal and plant symbiotic culture substrate according to claim 7, wherein: stirring, airing and grinding three steps are carried out for in proper order when ammonia water and brown coal mix, and the stirring mode sets up to just reversing the rotation of alternating.
9. The formula and preparation process of culture medium for symbiosis of fungi and plants according to claim 7 are characterized in that: and mixing and stirring the product obtained by mixing the ammonia water and the lignite, the product obtained by mixing the urea and the phosphoric acid and the rest substances in an equal-proportion quantitative adding mode, wherein the mixing and stirring time is 30 min.
10. A method of using the fungal and plant symbiotic culture substrate of claim 1, comprising the steps of:
s1, taking a certain amount of culture medium for symbiosis of fungi and plants, putting the culture medium into a container such as a bowl and the like, and sterilizing for later use;
s2, soaking the plant seeds in 0.1% potassium permanganate solution for 10 minutes for disinfection, and blotting the plant seeds with sterile paper for later use;
s3, digging a culture medium at the center of the pot on a superclean bench by using a sterile spoon for 4 cm, inoculating fungi to be researched or inspected, covering the medium for 1 cm, sowing the sterilized seeds, and covering the medium for 3 cm;
s4, placing the cultivation pot for inoculation and sowing in an aseptic or ventilated bright environment, pouring sterile water, and culturing while keeping the relative water content at 70-80%.
CN202111089610.XA 2021-09-17 2021-09-17 Formula and preparation process of culture medium for symbiosis of fungi and plants Pending CN113615536A (en)

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Application publication date: 20211109