CN113598200A - Stored pest plant source repellent and application thereof - Google Patents

Stored pest plant source repellent and application thereof Download PDF

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CN113598200A
CN113598200A CN202110906364.6A CN202110906364A CN113598200A CN 113598200 A CN113598200 A CN 113598200A CN 202110906364 A CN202110906364 A CN 202110906364A CN 113598200 A CN113598200 A CN 113598200A
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volatile oil
plant
derived
emulsifier
repellent
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CN113598200B (en
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桑育黎
郝延军
刘景玉
刘玉峰
白丽
石磊
辛跃强
张昕茹
李秋钰
戴璐
王沛
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Liaoning University
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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
    • A01N25/02—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests containing liquids as carriers, diluents or solvents
    • A01N25/04—Dispersions, emulsions, suspoemulsions, suspension concentrates or gels
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
    • A01N65/08—Magnoliopsida [dicotyledons]
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
    • A01N65/08—Magnoliopsida [dicotyledons]
    • A01N65/22—Lamiaceae or Labiatae [Mint family], e.g. thyme, rosemary, skullcap, selfheal, lavender, perilla, pennyroyal, peppermint or spearmint
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
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Abstract

The invention relates to a stored pest plant source repellent and application thereof. The technical scheme is as follows: the storage pest plant-derived repellent is an emulsion prepared from plant-derived volatile oil, an emulsifier and water; one or more of traditional Chinese medicine patchouli volatile oil, traditional Chinese medicine mint volatile oil and traditional Chinese medicine forsythia volatile oil are taken to form plant-derived volatile oil, span-20 and tween-80 are used for preparing an emulsifier with the HLB value of 9.0-15.0, the adding proportion of the emulsifier is 4-10% of the total volume of the emulsion, and the adding proportion of the plant-derived volatile oil is 20-50% of the total volume of the emulsion. The emulsion provided by the invention can effectively prolong the action time of repelling stored pests and improve the repelling rate.

Description

Stored pest plant source repellent and application thereof
Technical Field
The invention belongs to the field of pesticides, and particularly relates to a stored pest plant source repellent taking traditional Chinese medicine volatile oil as a raw material and application thereof.
Background
Grains and traditional Chinese medicinal materials rich in protein, starch and grease are extremely easy to be attacked by insect pests in the storage process, so that the nutritional value or the medicinal value is lost, and therefore, the insect pest control is well performed in the storage process, and the method has important practical significance for protecting resources and ensuring the safety of food and medicines.
At present, the pesticide for controlling the stored pests is mainly chemical pesticide, and the wide use of the chemical pesticide brings many negative problems of environmental pollution, ecological damage and the like, so that the control of the stored pests becomes more difficult, people gradually shift the focus of the control research of the stored pests to novel, efficient and safe botanical pesticides, and the screening of volatile oil with the functions of repelling and poisoning the pests is an effective way for developing the botanical pesticides. Chemical components in plants are naturally occurring, are generally easy to degrade in natural environment, have relatively low toxicity, and are not easy to generate resistance to pests. Therefore, one or more plant volatile oils are utilized to develop safe and effective botanical insecticides, which meet the current market demand and the development trend of future insecticide research.
Disclosure of Invention
The invention aims to establish a stored pest plant-derived repellent prepared from traditional Chinese medicine volatile oil serving as a raw material.
The technical scheme adopted by the invention is as follows: the storage pest plant-derived repellent is an emulsion prepared from plant-derived volatile oil, an emulsifier and water; the composition by volume percentage is as follows: 20-50% of plant source volatile oil, 4-10% of emulsifier and the balance of water; the plant source volatile oil is one or the combination of more than two of patchouli volatile oil, mint volatile oil and forsythia volatile oil; the emulsifier is prepared by mixing span-20 and tween-80.
Preferably, the stored pest plant-derived repellent is prepared by combining patchouli volatile oil and mint volatile oil.
More preferably, in the storage pest plant-derived repellent, the plant-derived volatile oil is 55.5% by volume of the patchouli volatile oil and 44.5% by volume of the mint volatile oil.
Preferably, the emulsifier is an emulsifier which is prepared by mixing span-20 and tween-80 and has an HLB value of 9.0-15.0.
More preferably, the above-mentioned stored-pest plant-derived repellent, wherein the emulsifier is an emulsifier having an HLB value of 12.0 prepared by mixing span-20 and tween-80.
Preferably, the stored pest plant-derived repellent comprises the following components in percentage by volume: 43.2 percent of plant-derived volatile oil, 8 percent of emulsifier and the balance of water.
The invention provides application of a stored pest plant-derived repellent in repelling stored pests.
Preferably, the storage pest is tribolium castaneum.
The invention has the beneficial effects that:
1. the invention relates to a stored pest plant-derived repellent, which consists of an oil phase, a water phase and an emulsifier, wherein the plant-derived volatile oil is prepared into an emulsion form, so that oil-water two-phase liquid which are not mutually soluble can be prepared, and one phase is dispersed in the other phase of liquid in a droplet state.
2. According to the stored pest botanical repellent, the traditional Chinese medicines of patchouli volatile oil, mint volatile oil and forsythia volatile oil are prepared into emulsion according to the optimal proportion, so that the stability of the botanical volatile oil can be improved, the volatilization speed can be reduced, and the action time and the repellency rate for repelling stored pests can be prolonged.
3. After screening the repellent activity of a large amount of plant volatile oil, the invention discovers that the traditional Chinese medicine patchouli, mint and forsythia volatile oil has good repellent activity to the worldwide storage pest tribolium castaneum, and the three traditional Chinese medicines have high volatile oil content and stable composition and content of volatile oil components, can be industrially extracted, and can realize commercial supply of the volatile oil.
4. The storage pest plant-derived repellent disclosed by the invention is prepared by taking traditional Chinese medicines of patchouli, mint and forsythia volatile oil as raw materials, and can effectively prolong the action time and action efficacy of repelling the storage pest tribolium castaneum.
Drawings
FIG. 1 shows the results of the determination of the activity of patchouli volatile oil to repel tribolium castaneum.
FIG. 2 shows the results of the activity of peppermint volatile oil against tribolium castaneum.
FIG. 3 shows the results of the activity of forsythia suspense volatile oil in repelling tribolium castaneum.
FIG. 4 is a three-dimensional effect surface and contour map of the repellent rate (PR) of patchouli volatile oil (A), peppermint volatile oil (B) and forsythia volatile oil (C) to tribolium castaneum.
FIG. 5 is a three-dimensional effect surface and contour plot of emulsifier usage (A), plant-derived volatile oil usage (B), and stability constant (Ke).
FIG. 6 shows the repellency activity of the plant-derived volatile oil against Tripsammosilene rubescens.
Figure 7 is the repellency activity of stored pest plant-derived repellents against tribolium castaneum at optimal ratios (same letters are marked on bar graphs indicating no significant difference in ANOVA test and Tukey (P >0.05) levels).
Detailed Description
Example 1 stored Pest botanical repellent
The stored pest plant source repellent comprises the following components in percentage by volume: 20-50% of plant source volatile oil, 4-10% of emulsifier and the balance of water.
The plant source volatile oil is one or the combination of more than two of patchouli volatile oil, mint volatile oil and forsythia volatile oil.
The emulsifier is prepared by mixing span-20 and tween-80, and the HLB value of the emulsifier is 9.0-15.0.
Optimization of plant source volatile oil composition
1. Method for measuring repellent activity
Breeding the tribolium castaneum: the method comprises the steps of feeding the tribolium castaneum into a 0.5L glass bottle, feeding flour and yeast (10: 1, w/w) which are uniformly mixed, selecting 30-40 heads of active and healthy tribolium castaneum adults into the glass bottle filled with food, binding cotton cloth on the opening of the glass bottle by a rubber band, and culturing the glass bottle in a constant-temperature and constant-humidity box with the temperature of 30 +/-1 ℃ and the relative humidity of 70-80%. The laying time of the tribolium castaneum in the environment is about 4 days, and the generation is finished for about 27 days. Insects of 1-2 weeks of age were used without sex differentiation in all experiments.
And (3) testing the repellent activity: cutting circular filter paper with diameter of 9cm into two halves, and sucking 500 μ L (78.63, 15.73, 3.15, 0.63, 0.13 nL/cm) n-hexane diluent of plant volatile oil with pipette gun2) Dropping on half of the filter paper, treating the other half with n-hexane as control, air drying for 30s, and sticking on the bottom of the culture dish with solid glue. 20 test insects are poured into a culture dish, the number of the test insects staying on filter paper of the administration group and the control group is observed and recorded, the repellency (%) is calculated according to a formula, and the steps are carried out for five times in parallel. The calculation formula of the repellency rate PR (%) is as follows:
Figure BDA0003201665430000031
wherein, Nc: number of insects in control group, Nt: the number of insects in the group to be administered.
2. Determination of optimal ratio of three traditional Chinese medicine volatile oils in plant-derived volatile oil
2.1) determination results of repellent activity of three traditional Chinese medicine volatile oils
The determination is carried out according to a determination method of repellent activity,the results of the activity of patchouli volatile oil for repelling the pseudocorn gristle are shown in figure 1, the results of the activity of mint volatile oil for repelling the pseudocorn gristle are shown in figure 2, and the results of the activity of forsythia volatile oil for repelling the pseudocorn gristle are shown in figure 3. The results show that: the traditional Chinese medicine patchouli volatile oil, the mint volatile oil and the forsythia volatile oil have repellent activity on the pseudobulb beetle, the repellent rate is in positive correlation with the administration dosage, and the repellent rate is reduced along with the prolonging of the action time. At 78.63nL/cm2In the high-dose group, the repellent rate of the patchouli volatile oil to the tribolium castaneum is over 50 percent within 10 hours after the administration, and the repellent rate of the peppermint volatile oil and the forsythia volatile oil to the tribolium castaneum is over 6 hours after the administration.
2.2) screening the mixture ratio of three Chinese medicinal volatile oils in the plant-derived volatile oil
On the basis of 2.1) three traditional Chinese medicine volatile oil repelling experiments, the using amount of volatile oil of cablin potchouli herb, mint and fructus forsythiae is taken as a factor to be investigated, namely the using amount (A) of the volatile oil of cablin potchouli herb; the amount of the mint volatile oil (B); and (3) performing 3-factor star point Design (CCD-RSM star point Design effect surface optimization is performed by adopting Design Expert 8.0 software), wherein the dosage ranges of the three volatile oils are 0-34 uL, and the optimal formula of the volatile oil with the maximum repellent rate is investigated by taking the repellent rate (PR) as an index. The optimized factors and levels of the prescription of the plant-derived volatile oil are shown in table 1, the optimized experimental design and results of the prescription are shown in table 2, the variance analysis of repellent experimental data is shown in table 3, and the related coefficients of the optimized factors of the prescription are shown in table 4.
TABLE 1 preferred factors and levels of the formula of plant-derived essential oils
Figure BDA0003201665430000041
TABLE 2 preferred experimental design and results of plant-derived essential oil formulation
Figure BDA0003201665430000042
TABLE 3 variance analysis table of experimental data for repelling plant-derived volatile oil
Figure BDA0003201665430000051
TABLE 4 correlation coefficient of each preferable factor of plant-derived volatile oil prescription
Figure BDA0003201665430000052
According to the above results, the repellency rate (Y) was binomially fitted to each factor, and the result was PR 76.33+6.83A +0.56B-6.35C-2.25AB +3.25AC +1.75BC-7.02A2-5.68B2-0.35C2. Three-dimensional effect surface analysis (RSM) was performed according to the fitted equation as shown in fig. 4.
According to the experimental data of tables 1 to 4 and fig. 1 to 4, Design Expert software is used for optimizing the optimal repellent rate, the optimal formula of the dosage of three traditional Chinese medicine volatile oils is given, and the results show that the dosages of the patchouli volatile oil (A), the mint volatile oil (B) and the forsythia volatile oil (C) are respectively 18.22uL and 14.63uL, the repellent rate is the maximum when the dosage is 0uL, and the prediction is 86.6607%, as shown in table 5. The optimum formulation was tested and the results are shown in Table 6.
Table 5 optimal repellent prescription optimization value and repellent rate prediction value of three traditional Chinese medicine volatile oils
Figure BDA0003201665430000061
Table 6 verification results of optimal repellent optimized prescription of three traditional Chinese medicine volatile oils
Figure BDA0003201665430000062
In conclusion, the optimal mixture ratio of the patchouli volatile oil (A), the mint volatile oil (B) and the forsythia volatile oil is 18.22uL, 14.63uL and 0uL respectively, namely the patchouli volatile oil accounts for 55.5 percent of the total volume of the plant-derived volatile oil, and the mint volatile oil accounts for 44.5 percent of the total volume of the plant-derived volatile oil.
Optimization of emulsifiers
1. Screening of emulsifiers for optimal HLB value
1.1) preparing the emulsifier with a series of HLB values.
Span-20 (HLB: 8.6) and Tween-80 (HLB: 15.0) were mixed to prepare a series of HLB emulsifiers, and the amounts of Tween-80 and span-20 were as shown in Table 7. The HLB values of the prepared emulsifier are respectively 9.0, 10.0, 11.0, 12.0, 13.0, 14.0 and 15.0. The calculation formula of the hydrophile-lipophile balance (HLB) of the emulsifier is as follows:
Figure BDA0003201665430000063
wherein HLB isTIs the HLB value of span-20, WTIs the amount of span-20
HLBSIs the HLB value, W, of Tween-80SIs the dosage of tween-80
TABLE 7 dosage of Tween-80 and span-20 in the emulsifier with HLB value series
Figure BDA0003201665430000071
1.2) determination of optimal HLB emulsifier
Taking 7 test tubes with plugs and 10mL, adding plant volatile oil 1mL (the plant volatile oil comprises herba Agastaches volatile oil accounting for 55.5% of the total volume and herba Menthae volatile oil accounting for 44.5% of the total volume), adding emulsifying agents with different HLB values 0.5mL, and shaking vigorously for 10 s. Then distilled water was slowly added along the tube wall to 5mL and shaken 30 times to form an emulsion. Oil: water: milk (volume ratio) ═ 2: 7: 1. standing for 10, 30, 60min, 5h, 24h and 48h, and observing and recording the layering condition of the emulsion in each test tube.
The stability constant Ke, which is the percentage of change in optical density before and after centrifugation of the emulsion, was determined for emulsions without any separation, and the stability constant Ke was used as a quantitative index for studying the stability of the emulsion.
Centrifuging the emulsion in a centrifuge tube at 3000r/min for 15min, and collecting the subnatantAdding 29uL of the sample into a 10mL measuring flask, adding deionized water to the scale, measuring the absorbance A at 500nm with the deionized water as a blank, and directly measuring the absorbance A without centrifugation0And calculating the value of the stability constant Ke. Determining the emulsifier with the optimal HLB value. The results are shown in Table 8
Figure BDA0003201665430000072
A0Absorbance values measured for dilutions of the emulsion at a set wavelength of 500nm
A is the absorbance value of the diluted solution of the emulsion after centrifugation at the same wavelength
The smaller the Ke, the smaller the change of the emulsion before and after centrifugation, the more stable the emulsion.
TABLE 8 demixing of mixed oil and water emulsions at different HLB values (Unit: mL)
Figure BDA0003201665430000073
Figure BDA0003201665430000081
From the results of table 8, the optimum HLB value of the emulsifier was determined to be 12.0. That is, span-20 (HLB: 8.6) and Tween-80 (HLB: 15.0) were mixed to prepare an emulsifier having an HLB value of 12.0.
(III) screening of emulsifier addition ratio
The method comprises the steps of measuring the plant-derived volatile oil (the plant-derived volatile oil comprises 55.5% of the total volume of the patchouli volatile oil and 44.5% of the total volume of the mint volatile oil) which accounts for 20% of the total volume (5mL) of the emulsion, selecting an emulsifier with the HLB value of 12.0, preparing the emulsion according to different emulsifier addition volumes (0.5, 0.4, 0.3 and 0.2mL), standing for 5h and 24h, observing the layering condition, determining the emulsifier addition proportion, and obtaining the result shown in Table 9.
TABLE 9 screening of emulsifier amounts (Unit: mL)
Figure BDA0003201665430000082
When the HLB of the emulsifier is 12.0, the volume of the plant-derived volatile oil in the emulsion is 20%, and the emulsion layering condition observation for 5h and 24h preliminarily determines that the adding volume range of the emulsifier is 0.4-0.5 mL, namely, the adding proportion of the emulsifier in the emulsion is 8-10%.
(IV) screening of addition proportion of plant-derived volatile oil
Selecting an emulsifier with the HLB value of 12.0, wherein the adding volume of the emulsifier is 0.5mL, adding 1.0, 1.5, 2.0 and 2.5mL of plant-derived volatile oil with different volumes (the plant-derived volatile oil comprises 55.5 percent of the patchouli volatile oil and 44.5 percent of the mint volatile oil), preparing an emulsion with the total volume of 5mL, standing for 5h and 24h to observe the layering condition, and inspecting the adding proportion of the plant-derived volatile oil, wherein the result is shown in Table 10.
TABLE 10 screening of the amount of botanical volatile oil used (Unit: mL)
Figure BDA0003201665430000083
When the HLB value of the emulsifier is 12.0 and the adding volume of the emulsifier is 0.5mL, the maximum adding volume of the plant-derived volatile oil is preliminarily determined to be 2.0-2.5 mL, and the proportion of the plant-derived volatile oil contained in the emulsion is 40-50%.
(V) determining optimal emulsion prescription by using star point design-response surface method (CCD-RSM)
Based on the single factor investigation of the prescription of the emulsion, the star point design is carried out and the optimal prescription of the emulsion is determined by combining the three-dimensional response surface. Taking the addition amount of the emulsifier with HLB value of 12.0 and the plant-derived volatile oil as investigation factors, namely, taking the dosage (A) of the emulsifier and the dosage (B) of the plant-derived volatile oil (the plant-derived volatile oil comprises 55.5 percent of the patchouli volatile oil and 44.5 percent of the mint volatile oil) to determine the optimal emulsion formula by 2-factor CCD-RSM. The total volume of the prepared emulsion is 5mL, wherein the dosage range of the emulsifier is 0.4-0.5 mL, the dosage range of the plant source volatile oil is 2.0-2.5 mL, the stability constant Ke (Y) is used as an index, the optimal emulsion formula optimization factors and levels are shown in table 11, the formula optimization test design and results are shown in table 12, the emulsion formula screening test data analysis of variance is shown in table 13, and the formula optimization factors of correlation coefficients are shown in table 14.
TABLE 11 optimal emulsion formulation preference factors and levels of plant-derived essential oils
Figure BDA0003201665430000091
TABLE 12 optimized formulation test design and results for plant-derived volatile oil emulsions
Figure BDA0003201665430000092
Figure BDA0003201665430000101
TABLE 14 emulsion formulation optimization factor correlation of each factor
Figure BDA0003201665430000102
As can be seen from tables 11 to 14, the stability constant Ke (y) was binomially fitted to each factor, and as a result, Ke was 52.15+10.97A to 7.53B to 9.38AB to 17.15A2-15.77B2Three-dimensional effect surface analysis (RSM) was performed according to the fitted equation as shown in fig. 5.
From tables 11-14, the optimum emulsion formulation was determined as follows: the dosage of the emulsifier (A) and the dosage of the plant-derived volatile oil (B) are respectively 0.40mL and 2.16mL, namely the emulsifier accounts for 8% of the total volume of the emulsion, and the plant-derived volatile oil accounts for 43.2% of the total volume of the emulsion. The predicted value of the stability constant (Ke) at this time was 0.09%, as shown in table 15. The verification experiments were performed according to the optimized recipe, and the verification results are shown in table 16.
TABLE 15 optimal emulsion formula optimization values and stability constant prediction values for plant-derived volatile oils
Figure BDA0003201665430000103
Figure BDA0003201665430000111
TABLE 16 optimized formula verification test results for plant-derived volatile oils
Figure BDA0003201665430000112
By combining the above analysis, the best prescription of the stored pest plant-derived repellent is as follows: the botanical volatile oil is prepared from 55.5 percent by volume of patchouli volatile oil and mint volatile oil: 44.5% of the composition. The emulsifier is an emulsifier which is prepared by mixing span-20 and Tween-80 and has the HLB value of 12.0. The emulsifier accounts for 8% of the total volume of the emulsion, and the plant-derived volatile oil accounts for 43.2% of the total volume of the emulsion, and the mixture is uniformly mixed to prepare the emulsion.
Example 2 determination of Activity of a botanical repellent against Tripsammosilene rubescens for stored pests
The preparation method of the stored pest plant-derived repellent comprises the following steps:
1) the composition of the plant-derived volatile oil is as follows: and (3) uniformly mixing 18.22uL of the traditional Chinese medicine patchouli volatile oil and 14.63uL of the traditional Chinese medicine mint volatile oil to obtain the volatile oil, wherein the volatile oil accounts for 55.5 percent of the total volume of the patchouli volatile oil and 44.5 percent of the total volume of the mint volatile oil.
2) Preparing an emulsifier: mixing span-20 and Tween-80 to prepare the emulsifier with the HLB value of 12.0.
3) According to the volume percentage, 8 percent of emulsifier and 43.2 percent of plant-derived volatile oil are uniformly mixed to prepare the emulsion.
The repellent activity of the plant-derived volatile oil prepared in the step 1) on the tribolium castaneum is measured, and the result is shown in fig. 6.
The repellent activity of the plant-derived volatile oil emulsion prepared in the step 3) on the tribolium castaneum is measured, and the result is shown in fig. 7.
As can be seen from FIGS. 6 and 7, the repellent activity of the plant-derived volatile oil prepared in the optimum ratio was compared with that of the volatile oil of Pogostemon cablin, Mentha haplocalyx and forsythiae fructus at 78.63nL/cm2In the high-dose group, within 24 hours of administration, the repellent rate of the plant-derived volatile oil is higher than 50 percent. At 78.63nL/cm2In the high-dose group, the repellent action time and the action intensity of the plant-derived volatile oil emulsion and the plant-derived volatile oil are not obviously different, but are 15.73nL/cm2、3.15nL/cm2、0.63nL/cm2In the dosage group, the plant-derived volatile oil emulsion keeps the repellent rate to be more than 50% within 12h, 8h and 4h respectively, and the action intensity of the plant-derived volatile oil emulsion is higher than that of the plant-derived volatile oil emulsion in the same dosage group, so that the action time of the plant-derived volatile oil emulsion for repelling the stored insects can be prolonged after the emulsion is prepared.
In order to eliminate the influence of the emulsifier on the activity test of repelling the tribolium castaneum, the emulsifier with the HLB value of 12.0 is selected and diluted into 78.63nL/cm by n-hexane2The concentration was measured to determine the effect of repelling tribolium castaneum, and the results are shown in Table 17.
TABLE 17 emulsifier repellent to Tripsammophyte
Figure BDA0003201665430000121
The experimental result shows that the repellent rate of the emulsifier is negative or zero, which indicates that the pure emulsifier has no repellent activity to the tribolium castaneum, so that the measurement result of the repellent activity of the plant-derived volatile oil emulsion to the tribolium castaneum is not influenced.

Claims (8)

1. The stored pest plant-derived repellent is characterized by being an emulsion prepared from plant-derived volatile oil, an emulsifier and water; the composition by volume percentage is as follows: 20-50% of plant source volatile oil, 4-10% of emulsifier and the balance of water; the plant source volatile oil is one or the combination of more than two of patchouli volatile oil, mint volatile oil and forsythia volatile oil; the emulsifier is prepared by mixing span-20 and tween-80.
2. The stored pest plant-derived repellent according to claim 1, wherein the plant-derived volatile oil is a combination of a patchouli volatile oil and a mint volatile oil.
3. The stored-grain insect plant-derived repellent according to claim 2, wherein the plant-derived volatile oil comprises 55.5% by volume of patchouli volatile oil and 44.5% by volume of mint volatile oil.
4. The stored pest plant-derived repellent according to claim 1, wherein the emulsifier is an emulsifier which is prepared by mixing span-20 and tween-80 and has an HLB value of 9.0-15.0.
5. The stored pest plant-derived repellent according to claim 4, wherein said emulsifier is an emulsifier having an HLB value of 12.0 formulated as a mixture of span-20 and Tween-80.
6. The stored pest plant-derived repellent according to any one of claims 1 to 5, wherein the stored pest plant-derived repellent consists of, by volume percent: 43.2 percent of plant-derived volatile oil, 8 percent of emulsifier and the balance of water.
7. Use of a stored pest plant-derived repellent according to claim 1 for repelling stored pests.
8. The use according to claim 7, wherein the storage pest is tribolium castaneum.
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