CN113559454A

CN113559454A - Biodegradation method for enhancing soil degradation of carpropamid

Info

Publication number: CN113559454A
Application number: CN202110774783.9A
Authority: CN
Inventors: 何健; 豆叶枝; 李菊颖; 孔德洋; 许静; 余佳; 张悦清; 曹莉
Original assignee: Nanjing Institute of Environmental Sciences MEE
Current assignee: Nanjing Institute of Environmental Sciences MEE
Priority date: 2021-07-09
Filing date: 2021-07-09
Publication date: 2021-10-29
Anticipated expiration: 2041-07-09
Also published as: CN113559454B

Abstract

The invention discloses a biodegradation method for enhancing soil degradation of carpropamid, which comprises the following steps: culture of S1 microorganism: culturing and fermenting multiple microorganisms in a microorganism culture medium for 3-4 days, mixing fermented bacteria liquid to obtain a mixed liquid, and culturing in a culture solution to obtain a stable microorganism thallus mixed liquid; s2 soil pretreatment: laying a group of air pipelines at the depth of 0.8-1m in the soil every 3m, and covering a rainproof shed above the soil; s3 microbial application; s4 is supplemented by microorganisms. The biodegradation method realizes effective degradation of pesticide residues in soil, particularly aiming at degradation of the cypionamide, by compounding various microorganisms, can improve the soil quality, is favorable for promoting the degradation of the cypionamide in the soil by the microorganisms, and has important significance for protecting the ecological environment and the living health of people.

Description

Biodegradation method for enhancing soil degradation of carpropamid

Technical Field

The invention relates to the technical field of soil pollutant degradation, in particular to a biodegradation method for enhancing soil degradation of carpropamid.

Background

The pesticide residue is in agricultural production and the phenomenon that a part of pesticide directly or indirectly remains in grains, vegetables, fruits, livestock products, aquatic products and soil and water after the pesticide residue is applied, the pesticide residue problem is generated along with the mass production and wide use of the pesticide, only a small part of the pesticide can act on plant plants after being sprayed, and the other most part of the pesticide can permeate into soil, if the pesticide has serious influence on the soil and the water due to improper treatment, the pesticide has adverse influence on the production and life of people, the pesticide can seriously enter the human body through food chain enrichment, the health of the human body is harmed, and the wide attention of people is caused at the present stage, so that the pesticide residue in the soil needs to be degraded by reasonable means.

The degradation methods and degradation rates of different pesticides are different, common pesticide residue degradation methods comprise a physical method, a chemical method and a biological method, the physical method usually utilizes the physical properties of the pesticides, such as water solubility, light instability, thermal instability and other principles to remove pesticide residues, and the physical method generally comprises a soaking cleaning method, a peeling method, a sunlight irradiation method, a storage method, an adsorption method and the like; the chemical method is that the double bonds of pesticide molecules are broken by using the strong oxidation of hydrogen peroxide, ozone, hypochlorite strong oxidant or free radicals, the benzene rings are opened, the molecular structure of the pesticide molecules is destroyed, and corresponding acid, alcohol, amine or oxide thereof and the like are generated; the degradation of pesticide residue by microorganisms or enzymes is a hot spot of research in recent years, wherein the research on bacteria and fungi is relatively deep, the adaptability of bacteria is strong, mutant strains are easy to induce and take an important place, and a plurality of microorganisms capable of degrading pesticides can be found through technologies such as enrichment culture or separation screening.

Patent CN109651015A discloses a bioactive agent capable of degrading pesticide residues in soil, a preparation method and application thereof, wherein the bioactive agent comprises the following specific components in percentage by mass: 10-15 parts of compound bacteria fermentation product, 15-20 parts of compound amino acid, 20-35 parts of humic acid, 20-30 parts of volcanic ash and 25-35 parts of cassava vinasse; the composite microbial fermentation product is prepared by separately fermenting microbial agents containing bacillus amyloliquefaciens and bacillus subtilis, wherein the bacillus amyloliquefaciens is more than or equal to 2.0 hundred million/g and the bacillus subtilis is more than or equal to 2.0 hundred million/g after the fermentation products are mixed. The active agent has stable structure, wide function and no toxic or side effect, has good application prospect in the aspects of agricultural product quality safety, agricultural ecological environment protection and the like, can effectively degrade pesticide residues in soil in agricultural production, has obvious effect on degrading herbicide residues, inhibits the absorption of plants on the pesticide residues, can promote the growth of the plants, and improves the yield and the quality. But does not play a good role in degrading the cypionamide.

The cyprodinil belongs to a cyclopropane carboxylic acid plant growth regulator, has a synergistic effect when mixed with ethephon, and is mainly used for the growth of crops such as cotton, cereal and the like. Studies show that the carpropamid is easily degraded into 2, 4-dichloroaniline in soil, and the metabolite has extremely high toxicity to aquatic organisms and may have adverse effects on the environment. At present, the research on the method for degrading the cyprodinil is less, so that the method for degrading the cyprodinil by combining the hydrolysis, photolysis and microbial characteristics of the cyprodinil is needed to provide a suitable method for enhancing the biodegradation of the cyprodinil by soil.

Disclosure of Invention

In view of the above-mentioned problems, the present invention provides a biodegradation method for enhancing soil degradation of carpropamid.

The technical scheme of the invention is as follows:

a biodegradation process for enhancing the degradation of carpropamid by soil comprising the steps of:

culture of S1 microorganism: culturing multiple microorganisms in a microorganism culture medium for 3-4 days to obtain a microorganism with a microorganism content of 1-3 × 10⁸Mixing the fermented bacteria liquid to obtain a mixed liquid, and putting the mixed liquid into a culture solution to culture to obtain a stable microbial thallus mixed liquid;

s2 soil pretreatment: adjusting the water content of the soil to be treated to 55-60%, controlling the soil temperature at 26 +/-3 ℃, laying a group of air pipelines (1) at the depth of 0.8-1m in the soil every 3m, and covering a rain-proof shed above the soil;

s3 microbial application: uniformly spraying the microbial thallus mixed solution obtained in the step S1 on the surface of the soil at the dosage of 10-13 kg/mu, simultaneously starting gas injection, and starting lighting irradiation at night for 10-15 days;

s4 microbial supplementation: uniformly injecting the soil between every two groups of air pipelines (1) at the depth of 0.5-1m by a high-pressure pump gun, wherein the injection amount is 5-8 kg/mu, and the injection amount is once every 7 days for 3 times.

Further, the plurality of microorganisms in the step S1 includes, in parts by weight: 25-28 parts of pseudomonas, 17-19 parts of bacillus, 6-8 parts of trichoderma viride, 5-6 parts of fusarium and 1-2 parts of penicillium oxalicum, and the effective degradation of pesticide residues in soil, especially the degradation of carpropamid, is realized by compounding various microorganisms.

Further, the components of the microbial culture medium in the step S1 include, by weight: 100 parts of water, 5-10 parts of glucose, 12-15 parts of agar, 3-4 parts of edible oil, 2-3 parts of peach gum and 7-8 parts of rice husk.

Further, the components of the culture solution in the step S1 include, by weight: 45-50 parts of compound amino acid, 3-5 parts of monopotassium phosphate, 1-3 parts of magnesium sulfate, 5-7 parts of sodium chloride, 3-4 parts of calcium chloride and 10 parts of biochar, and the pH value is adjusted to 7.2-7.5.

Further, the method for culturing the mixed solution in the microbial culture medium in step S1 specifically includes:

s1-1 culture container disinfection: adding culture solution into a sterile culture container, sterilizing with 110-120 deg.C high-temperature steam for 1h, vacuumizing to 0.1Pa, and naturally cooling to 31-33 deg.C;

s1-2 secondary fermentation: filtering the fermented microorganisms obtained in the step S1 respectively, mixing and uniformly stirring the filtered microorganism solution to obtain a mixed solution, and continuously fermenting for 24 hours at the temperature of 26-29 ℃;

s1-3 mixing: continuously injecting mixed liquid from the bottom of the culture container at the injection speed of 1kg/min and the weight ratio of the mixed liquid to the culture solution of 1:5, and continuously introducing mixed gas from the top of the culture container to form airflow stirring, wherein the injection speed of the mixed gas is 0.3-0.5L/min, and the components of the mixed gas are in volume ratio: 20% of oxygen, 1-2% of nitric oxide, 2-3% of carbon dioxide and the balance of nitrogen. The injected nitric oxide in the mixed gas is an important active molecule in the microorganism, has the functions of protecting the microorganism and improving the drug resistance of the microorganism, and simultaneously recycles harmful gases containing NO, such as smoke, tail gas and the like; likewise, CO₂Can improve the absorption of the microorganisms on nitrogen and phosphorus and also recycle the waste gas and the tail gas.

Furthermore, in the step S2, a plurality of branched air ducts are arranged at the left and right ends of the air pipeline at equal intervals, each branched air duct comprises a telescopic mechanism positioned at the center of the branched air duct and a fiber breathable layer sleeved on the periphery of the telescopic mechanism, the telescopic mechanism comprises a plurality of telescopic pipes fixedly connected, the tail end of the telescopic mechanism is provided with a ground breaking drill bit, each telescopic pipe comprises a front section pipe and a rear section pipe, the front section pipe and the rear section pipe are connected through a group of connecting rods, two ends of each connecting rod are provided with limiting slide blocks protruding upwards, the upper parts in the front section pipe and the rear section pipe are provided with slide grooves used for being in sliding connection with the limiting slide blocks, the outer ends, positioned at the limiting slide blocks, of the front section pipe are provided with elastic air bags, the outer ends, positioned at the limiting slide blocks, in the rear section pipe are also provided with elastic air bags, the two groups of elastic air bags are connected through elastic connecting pipes positioned below the connecting rods, and the two adjacent groups of telescopic pipes are connected through fixing blocks, the elastic air bag in the back end pipe of a preceding set of flexible pipe and the elastic air bag in the front end pipe of a back set of flexible pipe are through running through the elastic hose of fixed block connects, through the setting of branch air duct, can increase the area of ventilating, makes the activity of aerobic microorganisms improve greatly, promotes the biodegradation to middle ring propionicamide in soil.

Furthermore, the inside cavity of fixed block sets up, and its inner surface is equipped with a plurality of movable blocks, every group the movable block concatenation is a complete ring, and every group movable block is through the inner wall connection of a set of spring with the fixed block, should set up and to make telescopic machanism expand progressively a bit at the gas injection in-process and advance rather than disposable extension, make the gas distribution effect more abundant.

Further, the lamp light used in step S3 is a high-pressure mercury lamp or xenon lamp, and under the lighting condition, the biodegradation of the cypionamide by the microorganisms on the soil can be effectively promoted.

Further, the gas injected in step S3 is sterile air, and the gas injection amount is 30m³Per mu. d^-1In step S4, gas injection is performed only on the 2 nd day after the microorganism supplement injection, and the gas injection amount is 10m³Per mu. d^-1. Multiple insufflation can maximize microbial activity.

The invention has the beneficial effects that:

(1) the biodegradation method realizes effective degradation of pesticide residues in soil, particularly aiming at degradation of the carpropamid by compounding various different microorganisms, can promote fermentation of the microorganisms by improving a microorganism culture medium and a culture solution, enables the activity of the microorganisms to be higher, can improve the soil quality, improves the water content and the oxygen content of the soil, is beneficial to promoting degradation of the carpropamid in the soil by the microorganisms, and has important significance for protecting the ecological environment and the living health of people.

(2) Hair brushThe method for biological degradation starts from green clean production, the production cost is low, the injected nitric oxide in the mixed gas is an important active molecule in the microorganism, the effect of protecting the microorganism and improving the drug resistance of the microorganism is achieved, and meanwhile, harmful gases containing NO such as smoke, tail gas and the like are recycled; likewise, CO₂Can improve the absorption of the microorganisms on nitrogen and phosphorus and also recycle the waste gas and the tail gas.

(3) The biodegradation method of the invention also provides a pipeline for high-efficiency gas distribution, which can increase the ventilation area through the branch gas guide pipe, greatly improve the activity of aerobic microorganisms, promote the biodegradation of the cypiopropanamide in soil, and simultaneously gradually expand and advance a little by a little in the gas injection process instead of one-time extension by a telescopic mechanism, so that the gas distribution effect is more sufficient, and the gas distribution effect is improved.

Drawings

FIG. 1 is a schematic view of the gas distribution structure of the gas pipeline of the present invention;

FIG. 2 is a schematic view of the interior of the branched gas duct of the present invention;

FIG. 3 is a schematic view of the telescoping mechanism of the present invention;

FIG. 4 is a schematic view of the fixed block of the present invention;

FIG. 5 is a schematic view of the internal structure of the fixing block of the present invention;

figure 6 is a schematic representation of the degradation of carpropamid under different conditions in an example of the invention.

The device comprises a gas pipeline 1, a branch gas guide pipe 2, a telescopic mechanism 3, a telescopic pipe 31, a front section pipe 32, a rear section pipe 33, a connecting rod 34, a limiting slide block 35, a sliding chute 36, an elastic air bag 37, an elastic connecting pipe 38, an elastic hose 39, a fiber air-permeable layer 4, a ground breaking drill bit 5, a fixed block 6, a movable block 61 and a spring 62.

Detailed Description

Example 1

culture of S1 microorganism: culturing multiple microorganisms in microorganism culture mediumAnd (3) culturing and fermenting for 3d, wherein the various microorganisms comprise the following components in parts by weight: 25 parts of pseudomonas, 17 parts of bacillus, 8 parts of trichoderma viride, 6 parts of fusarium and 2 parts of penicillium oxalicum, wherein the microbial culture medium comprises the following components in parts by weight: 100 parts of water, 5 parts of glucose, 12 parts of agar, 4 parts of edible oil, 3 parts of peach gum and 8 parts of rice husk, wherein the content of microorganisms is 1 multiplied by 10⁸Mixing the fermented bacteria liquid to obtain a mixed liquid, putting the mixed liquid into a culture solution to culture to obtain a stable microbial thallus mixed liquid, wherein the weight ratio of a microbial culture medium to the culture solution is 1:2, and the components of the culture solution comprise, in parts by weight: 45 parts of compound amino acid, 3 parts of monopotassium phosphate, 1 part of magnesium sulfate, 7 parts of sodium chloride, 4 parts of calcium chloride and 10 parts of biochar, and the pH value is adjusted to 7.2;

specifically, the method for culturing the mixed solution in the microbial culture medium in step S1 includes:

s1-1 culture container disinfection: adding the culture solution into an aseptic culture container, sterilizing with 110 deg.C high-temperature steam for 1h, vacuumizing to 0.1Pa, and naturally cooling to 31 deg.C;

s1-2 secondary fermentation: filtering the plurality of fermented microorganisms obtained in the step S1 respectively, mixing and uniformly stirring the filtered microorganism bacterium solutions to obtain a mixed solution, and continuously fermenting for 24 hours at 26 ℃;

s1-3 mixing: continuously injecting mixed liquid from the bottom of the culture container at the injection speed of 1kg/min, wherein the weight ratio of the mixed liquid to the culture solution is 1:5, continuously introducing mixed gas from the top of the culture container to form airflow stirring, wherein the injection speed of the mixed gas is 0.3L/min, and the components of the mixed gas are in volume ratio: 20% of oxygen, 1% of nitric oxide, 2% of carbon dioxide and the balance of nitrogen.

S2 soil pretreatment: adjusting the water content of the soil to be treated to 55%, controlling the temperature of the soil to be 23 ℃, laying a group of air pipelines 1 at the depth of 0.8m in the soil every 3m, and covering a rain-proof shed above the soil;

s3 microbial application: uniformly spraying the microbial thallus mixed solution obtained in the step S1 on the surface of the soil at the dosage of 10 kg/mu, and simultaneously injecting gas, wherein the injected gas is sterile air, and the gas injection amount is 30m³Per mu. d^-1Starting light irradiation at night, wherein the used light is a xenon lamp and lasts for 15 days;

s4 microbial supplementation: uniformly injecting the soil between every two groups of air pipelines 1 at a depth of 0.5m by a high-pressure pump gun, wherein the injection amount is 8 kg/mu, the injection is performed once every 7 days for 3 times, and the gas is injected on the 2 nd day after the microorganisms are additionally injected, wherein the gas injection amount is 10m³Per mu. d^-1。

Example 2

This embodiment is substantially the same as embodiment 1, except that: the composition of the microorganisms varies in content.

Culture of S1 microorganism: separately culturing and fermenting a plurality of microorganisms in a microorganism culture medium for 3.5d, wherein the plurality of microorganisms comprise the following components in parts by weight: 26 parts of pseudomonas, 18 parts of bacillus, 7 parts of trichoderma viride, 5 parts of fusarium and 2 parts of penicillium oxalicum.

Example 3

Culture of S1 microorganism: separately culturing and fermenting a plurality of microorganisms in a microorganism culture medium for 4d, wherein the plurality of microorganisms comprise the following components in parts by weight: 28 parts of pseudomonas, 19 parts of bacillus, 6 parts of trichoderma viride, 6 parts of fusarium and 1 part of penicillium oxalicum.

Example 4

This embodiment is substantially the same as embodiment 1, except that: the microbial culture media vary in their component content.

Culture of S1 microorganism: the microbial culture medium comprises the following components in parts by weight: 100 parts of water, 7 parts of glucose, 13 parts of agar, 3 parts of edible oil, 3 parts of peach gum and 7 parts of rice husk, wherein the content of microorganisms is 2 multiplied by 10⁸Per gram.

Example 5

Culture of S1 microorganism: the microbial culture medium comprises the following components in parts by weight: 100 portions of water,10 portions of glucose, 15 portions of agar, 3 portions of edible oil, 2 portions of peach gum and 7 portions of rice husk, and the microbial content is 3X 10⁸Per gram.

Example 6

This embodiment is substantially the same as embodiment 1, except that: the culture medium has different component contents.

Culture of S1 microorganism: the culture solution comprises the following components in parts by weight: 48 parts of compound amino acid, 4 parts of monopotassium phosphate, 2 parts of magnesium sulfate, 6 parts of sodium chloride, 3 parts of calcium chloride and 10 parts of biochar, and the pH value is adjusted to 7.3.

Example 7

Culture of S1 microorganism: the culture solution comprises the following components in parts by weight: 50 parts of compound amino acid, 5 parts of monopotassium phosphate, 1 part of magnesium sulfate, 5 parts of sodium chloride, 3 parts of calcium chloride and 10 parts of biochar, and the pH value is adjusted to 7.5.

Example 8

This embodiment is substantially the same as embodiment 1, except that: in step S1, the culture method parameters of the mixed solution in the microorganism culture medium are different.

S1-1 culture container disinfection: adding culture solution into an aseptic culture container, sterilizing with 115 deg.C high-temperature steam for 1h, vacuumizing to 0.1Pa, and naturally cooling to 32 deg.C;

s1-2 secondary fermentation: filtering the plurality of fermented microorganisms obtained in the step S1 respectively, mixing and uniformly stirring the filtered microorganism bacterium solutions to obtain a mixed solution, and continuously fermenting for 24 hours at the temperature of 28 ℃;

s1-3 mixing: continuously injecting mixed liquid from the bottom of the culture container at the injection speed of 1kg/min, wherein the weight ratio of the mixed liquid to the culture solution is 1:5, continuously introducing mixed gas from the top of the culture container to form gas flow stirring, wherein the injection speed of the mixed gas is 0.4L/min, and the components of the mixed gas are in volume ratio: 20% of oxygen, 2% of nitric oxide, 2% of carbon dioxide and the balance of nitrogen.

Example 9

S1-1 culture container disinfection: adding the culture solution into an aseptic culture container, sterilizing with high-temperature steam at 120 deg.C for 1h, vacuumizing to 0.1Pa, and naturally cooling to 33 deg.C;

s1-2 secondary fermentation: filtering the plurality of fermented microorganisms obtained in the step S1 respectively, mixing and uniformly stirring the filtered microorganism bacterium solutions to obtain a mixed solution, and continuously fermenting for 24 hours at 29 ℃;

s1-3 mixing: continuously injecting mixed liquid from the bottom of the culture container at the injection speed of 1kg/min, wherein the weight ratio of the mixed liquid to the culture solution is 1:5, continuously introducing mixed gas from the top of the culture container to form gas flow stirring, wherein the injection speed of the mixed gas is 0.5L/min, and the components of the mixed gas are in volume ratio: 20% of oxygen, 1% of nitric oxide, 3% of carbon dioxide and the balance of nitrogen.

Example 10

This embodiment is substantially the same as embodiment 1, except that: the soil pretreatment parameters in step S2 are different.

S2 soil pretreatment: the water content of the soil to be treated is adjusted to 58 percent, the soil temperature is controlled to be 26 ℃, a group of air pipelines 1 are paved at the depth of 0.9m in the soil every 3m, and a rain-proof shed is covered above the soil.

Example 11

S2 soil pretreatment: the water content of the soil to be treated is adjusted to 60%, the soil temperature is controlled to be 29 ℃, a group of air pipelines 1 are paved at the depth of 1m in the soil every 3m, and a rain-proof shed is covered above the soil.

Example 12

This embodiment is substantially the same as embodiment 1, except that: the specific parameters in steps S3 and S4 are different.

S3 microbial application: the application of the fertilizer on the soil surface at 12 kg/muSpraying the mixed solution of the microbial cells obtained in the step S1 uniformly, and injecting gas with the amount of 30m, wherein the injected gas is sterile air³Per mu. d^-1Starting light irradiation at night, wherein the used light is a xenon lamp and lasts for 12 days; only in step S4

S4 microbial supplementation: uniformly injecting the soil between every two groups of air pipelines 1 at a depth of 0.7m by a high-pressure pump gun, wherein the injection amount is 7 kg/mu, the injection is performed once every 7 days for 3 times, and the gas is injected on the 2 nd day after the microorganisms are additionally injected, wherein the gas injection amount is 10m³Per mu. d^-1。

Example 13

S3 microbial application: uniformly spraying the microbial thallus mixed solution obtained in the step S1 on the surface of the soil at the dosage of 13 kg/mu, and simultaneously injecting gas, wherein the injected gas is sterile air, and the gas injection amount is 30m³Per mu. d^-1Starting lamp light irradiation at night, wherein the lamp light is a high-pressure mercury lamp and lasts for 10 days;

s4 microbial supplementation: uniformly injecting the soil between every two groups of air pipelines 1 at a depth of 1m through a high-pressure pump gun, wherein the injection amount is 5 kg/mu, the injection amount is once every 7 days and 3 times, and injecting gas at the 2 nd day after the microorganism supplement injection, wherein the gas injection amount is 10m³Per mu. d^-1。

Example 14

This embodiment is substantially the same as embodiment 1, except that:

step S2, a plurality of branched air ducts 2 are arranged at equal intervals at the left and right ends of the air pipeline 1, each branched air duct 2 comprises a telescopic mechanism 3 positioned at the center of the branched air duct and a fiber breathable layer 4 sleeved on the periphery of the telescopic mechanism 3, each telescopic mechanism 3 comprises a plurality of telescopic pipes 31 fixedly connected, the tail end of each telescopic mechanism 3 is provided with a ground breaking drill bit 5, each telescopic pipe 31 comprises a front-section pipe 32 and a rear-section pipe 33, the front-section pipe 32 and the rear-section pipe 33 are connected through a group of connecting rods 34, two ends of each connecting rod 34 are provided with upwards-protruding limiting slide blocks 35, the upper parts in the front-section pipe 32 and the rear-section pipe 33 are provided with sliding grooves 36 used for being in sliding connection with the limiting slide blocks 35, the outer ends, positioned at the limiting slide blocks 35, in the front-section pipe 32 are provided with elastic air bags 37, the outer ends, positioned at the limiting slide blocks 35, in the rear-section pipe 33 are also provided with elastic air bags 37, and the two groups of elastic air bags 37 are connected through elastic connecting pipes 38 positioned below the connecting rods 34, two adjacent groups of extension tubes 31 are connected through a fixed block 6, an elastic air bag 37 in a rear section tube 33 of a front group of extension tubes 31 is connected with an elastic air bag 37 in a front section tube 32 of a rear group of extension tubes 31 through an elastic hose 39 penetrating through the fixed block 6, the fixed block 6 is hollow inside, a plurality of movable blocks 61 are arranged on the inner surface of the fixed block, each movable block 61 is spliced into a complete ring, and each movable block 61 is connected with the inner wall of the fixed block 6 through a group of springs 62.

The working principle of the gas line 1 is as follows:

after the air pipeline 1 is ventilated, air enters the branch air guide tube 2, the air is firstly filled into the elastic air bag 37 in the front section tube 32, the elastic air bag 37 is inflated to extrude the limit slide block 35 to enable the limit slide block to slide along the slide groove 36, after the elastic air bag 37 in the front section tube 32 is filled with air, the elastic air bag 37 in the rear section tube 33 is inflated through the elastic connecting tube 38, similarly, the elastic air bag 37 is inflated to extrude the limit slide block 35 to enable the limit slide block 35 to slide along the slide groove 36, the extension of the telescopic tube 31 is completed, after one group of telescopic tubes 31 are completely extended, the air enters the elastic hose 39, the elastic hose 39 is inflated to extrude the movable block 61, the movable block 61 moves towards the inner wall of the fixed block 6 under the action of the spring 62, the ventilation area is increased, so that the elastic air bag 37 in the next group of telescopic tubes 31 is inflated, and the extension of the whole telescopic mechanism 3 is completed; the gas introduced subsequently enters the soil from the fibre gas-permeable layer 4.

Examples of the experiments

The soil samples of examples 1 to 13 were examined to determine the degradation effect by measuring the content of carpropamid in the soil, which was the red soil in Jiangxi soil contaminated with carpropamid to the same extent, and the examination results were as follows:

as can be seen from the data, the concentration of the cypiopropanamide in the examples 1-3 is low, and the half-life period is not very different, so that the content of the components of the microorganism can play a good role in degrading the cypionamide in the soil;

comparing examples 1, 4 and 5, it can be seen that the residual concentration of the carpropamid in example 4 is the lowest, so that the microbial mixture obtained by the fermentation of the microbial culture medium in example 4 has the best degradation effect on the carpropamid;

comparing examples 1, 6 and 7, it can be seen that the residual concentration of the carpropamid in example 7 is the lowest, therefore, the microorganism cultured with the culture solution of example 7 has the best effect of degrading the carpropamid, and the half-life is in direct proportion to the final concentration of the carpropamid;

as can be seen from comparison of examples 1, 8 and 9, the residual concentration of carpropamid in example 8 is the lowest because the secondary fermentation temperature selected in example 8 is suitable to maximize the activity of the microorganism and the NO and CO in the injected mixed gas₂The mixture ratio is more reasonable, the activity of the microorganism is further improved, and in example 9, CO is added₂Too high a concentration may be one of the reasons for poor degradation;

comparing examples 1, 10 and 11, it can be seen that the concentration of the residual carpropamid in the three groups of examples is inversely proportional to the half-life period, which indicates that the initial degradation speed is faster when the initial water content is high, but the residual carpropamid may be more at last, and the initial degradation speed is slower when the initial water content is low, but the carpropamid can be degraded more thoroughly;

comparing examples 1, 12 and 13, it can be seen that for the spraying mode of the microorganism, the proper spraying amount and spraying period are required, too much spraying in a short time and little spraying in a long time are not required, and the proper depth is also required to be selected for application, and the microorganism application parameter in example 12 is optimal;

as shown in fig. 6, the comparative example was an anaerobic state, i.e., the degradation effect obtained without using the gas line for gas injection, and it can be seen that the residual concentration of the carpropamide was higher in the anaerobic state, indicating that the microbial activity was decreased in the anaerobic state, and the gas line used in example 14 of the present invention can play a positive role in degrading the carpropamide by injecting gas into the soil.

Claims

1. A biodegradation process for enhancing the degradation of carpropamid by soil comprising the steps of:

2. The biodegradation process for enhancing the soil degradation of carpropamid according to claim 1, wherein the plurality of microorganisms of step S1 comprise, in parts by weight: 25-28 parts of pseudomonas, 17-19 parts of bacillus, 6-8 parts of trichoderma viride, 5-6 parts of fusarium and 1-2 parts of penicillium oxalicum.

3. The biodegradation process for enhancing the soil degradation of carpropamid according to claim 1, wherein the microbial culture medium of step S1 comprises, in parts by weight: 100 parts of water, 5-10 parts of glucose, 12-15 parts of agar, 3-4 parts of edible oil, 2-3 parts of peach gum and 7-8 parts of rice hull.

4. The biodegradation process for enhancing the soil degradation of carpropamid according to claim 1, wherein the components of the culture solution in step S1 comprise, in parts by weight: 45-50 parts of compound amino acid, 3-5 parts of monopotassium phosphate, 1-3 parts of magnesium sulfate, 5-7 parts of sodium chloride, 3-4 parts of calcium chloride and 10 parts of biochar, and the pH value is adjusted to 7.2-7.5.

5. The biodegradation process for enhancing the soil degradation of carpropamid according to claim 1, wherein the cultivation of the mixed liquor in a microorganism culture medium in step S1 is specifically:

s1-3 mixing: continuously injecting mixed liquid from the bottom of the culture container at the injection speed of 1kg/min and the weight ratio of the mixed liquid to the culture solution of 1:5, and continuously introducing mixed gas from the top of the culture container to form airflow stirring, wherein the injection speed of the mixed gas is 0.3-0.5L/min, and the components of the mixed gas are in volume ratio: 20% of oxygen, 1-2% of nitric oxide, 2-3% of carbon dioxide and the balance of nitrogen.

6. The biodegradation method for enhancing the soil degradation of carpropamid according to claim 1, wherein a plurality of branch air ducts (2) are arranged at equal intervals at the left and right ends of air duct (1) in step S2, said branch air ducts (2) comprise a telescopic mechanism (3) located at the center thereof and a fiber air-permeable layer (4) sleeved at the outer periphery of said telescopic mechanism (3), said telescopic mechanism (3) comprises a plurality of fixedly connected telescopic tubes (31), the end of said telescopic mechanism (3) is provided with a soil breaking drill (5), said telescopic tubes (31) comprise a front tube (32) and a rear tube (33), said front tube (32) and said rear tube (33) are connected by a set of connecting rods (34), said connecting rods (34) are provided with upwardly convex limiting sliders (35) at both ends thereof, the upper portions of said front tube (32) and said rear tube (33) are provided with chutes (36) for sliding connection with said limiting sliders (35), be located limiting slide block (35) outer end in front segment pipe (32) and be equipped with elasticity gasbag (37), be located limiting slide block (35) outer end in back segment pipe (33) and be equipped with elasticity gasbag (37) equally, two sets of elasticity gasbag (37) are connected through elastic connecting pipe (38) that are located connecting rod (34) below, and adjacent two sets of flexible pipes (31) are connected through fixed block (6), and elasticity gasbag (37) in back segment pipe (33) of a preceding set of flexible pipe (31) and the front segment pipe (32) of a back set of flexible pipe (31) are through running through the elasticity hose (39) of fixed block (6) are connected.

7. A biodegradation process for strengthening soil degrading carpropamides according to claim 8, characterized in that said fixed block (6) is internally hollow and has a plurality of movable blocks (61) on its inner surface, each group of said movable blocks (61) being spliced into a complete ring, each group of movable blocks (61) being connected to the inner wall of the fixed block (6) by means of a set of springs (62).

8. The biodegradation process for enhancing the degradation of carpropamid by soil according to claim 1, wherein the light used in step S3 is a high pressure mercury or xenon lamp.

9. The biodegradation process for enhancing the degradation of carpropamid by soil according to claim 1, wherein the gas injected in step S3 is sterile air injected in an amount of 30m³Per mu. d^-1In step S4, gas injection is performed only on the 2 nd day after the microorganism supplement injection, and the gas injection amount is 10m³Per mu·d^-1。