CN113546033A - Probiotic compound for promoting microecological balance and preparation method thereof - Google Patents
Probiotic compound for promoting microecological balance and preparation method thereof Download PDFInfo
- Publication number
- CN113546033A CN113546033A CN202110866279.1A CN202110866279A CN113546033A CN 113546033 A CN113546033 A CN 113546033A CN 202110866279 A CN202110866279 A CN 202110866279A CN 113546033 A CN113546033 A CN 113546033A
- Authority
- CN
- China
- Prior art keywords
- promoting
- probiotics
- probiotic compound
- humectant
- skin
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 239000006041 probiotic Substances 0.000 title claims abstract description 81
- 235000018291 probiotics Nutrition 0.000 title claims abstract description 81
- 230000000529 probiotic effect Effects 0.000 title claims abstract description 48
- 150000001875 compounds Chemical class 0.000 title claims abstract description 43
- 230000001737 promoting effect Effects 0.000 title claims abstract description 33
- 238000002360 preparation method Methods 0.000 title abstract description 9
- 239000003906 humectant Substances 0.000 claims abstract description 32
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 31
- 235000013406 prebiotics Nutrition 0.000 claims abstract description 29
- 150000003013 phosphoric acid derivatives Chemical class 0.000 claims abstract description 19
- -1 phosphate ester Chemical class 0.000 claims abstract description 17
- KIUKXJAPPMFGSW-DNGZLQJQSA-N (2S,3S,4S,5R,6R)-6-[(2S,3R,4R,5S,6R)-3-Acetamido-2-[(2S,3S,4R,5R,6R)-6-[(2R,3R,4R,5S,6R)-3-acetamido-2,5-dihydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-2-carboxy-4,5-dihydroxyoxan-3-yl]oxy-5-hydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-3,4,5-trihydroxyoxane-2-carboxylic acid Chemical class CC(=O)N[C@H]1[C@H](O)O[C@H](CO)[C@@H](O)[C@@H]1O[C@H]1[C@H](O)[C@@H](O)[C@H](O[C@H]2[C@@H]([C@@H](O[C@H]3[C@@H]([C@@H](O)[C@H](O)[C@H](O3)C(O)=O)O)[C@H](O)[C@@H](CO)O2)NC(C)=O)[C@@H](C(O)=O)O1 KIUKXJAPPMFGSW-DNGZLQJQSA-N 0.000 claims abstract description 14
- 229920002674 hyaluronan Chemical class 0.000 claims abstract description 14
- 229960003160 hyaluronic acid Drugs 0.000 claims abstract description 14
- YDNKGFDKKRUKPY-JHOUSYSJSA-N C16 ceramide Natural products CCCCCCCCCCCCCCCC(=O)N[C@@H](CO)[C@H](O)C=CCCCCCCCCCCCCC YDNKGFDKKRUKPY-JHOUSYSJSA-N 0.000 claims abstract description 13
- 229940106189 ceramide Drugs 0.000 claims abstract description 13
- ZVEQCJWYRWKARO-UHFFFAOYSA-N ceramide Natural products CCCCCCCCCCCCCCC(O)C(=O)NC(CO)C(O)C=CCCC=C(C)CCCCCCCCC ZVEQCJWYRWKARO-UHFFFAOYSA-N 0.000 claims abstract description 13
- VVGIYYKRAMHVLU-UHFFFAOYSA-N newbouldiamide Natural products CCCCCCCCCCCCCCCCCCCC(O)C(O)C(O)C(CO)NC(=O)CCCCCCCCCCCCCCCCC VVGIYYKRAMHVLU-UHFFFAOYSA-N 0.000 claims abstract description 13
- CRJGESKKUOMBCT-VQTJNVASSA-N N-acetylsphinganine Chemical class CCCCCCCCCCCCCCC[C@@H](O)[C@H](CO)NC(C)=O CRJGESKKUOMBCT-VQTJNVASSA-N 0.000 claims abstract description 12
- 229910019142 PO4 Inorganic materials 0.000 claims abstract description 10
- 239000010452 phosphate Substances 0.000 claims abstract description 10
- HNJBEVLQSNELDL-UHFFFAOYSA-N pyrrolidin-2-one Chemical compound O=C1CCCN1 HNJBEVLQSNELDL-UHFFFAOYSA-N 0.000 claims abstract description 10
- 239000004205 dimethyl polysiloxane Substances 0.000 claims abstract description 9
- 229920000435 poly(dimethylsiloxane) Polymers 0.000 claims abstract description 9
- 238000006243 chemical reaction Methods 0.000 claims description 35
- 239000000243 solution Substances 0.000 claims description 21
- ZMXDDKWLCZADIW-UHFFFAOYSA-N N,N-Dimethylformamide Chemical compound CN(C)C=O ZMXDDKWLCZADIW-UHFFFAOYSA-N 0.000 claims description 20
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 claims description 16
- 241000894006 Bacteria Species 0.000 claims description 14
- 238000002156 mixing Methods 0.000 claims description 14
- 238000010992 reflux Methods 0.000 claims description 12
- 238000002390 rotary evaporation Methods 0.000 claims description 12
- KSUWRPLSCJUDFR-UHFFFAOYSA-N 1-(3-chloro-2-hydroxypropyl)pyrrolidin-2-one Chemical compound ClCC(O)CN1CCCC1=O KSUWRPLSCJUDFR-UHFFFAOYSA-N 0.000 claims description 10
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 claims description 10
- 238000000855 fermentation Methods 0.000 claims description 10
- 230000004151 fermentation Effects 0.000 claims description 10
- 238000003756 stirring Methods 0.000 claims description 10
- FTSSQIKWUOOEGC-RULYVFMPSA-N fructooligosaccharide Chemical compound OC[C@H]1O[C@@](CO)(OC[C@@]2(OC[C@@]3(OC[C@@]4(OC[C@@]5(OC[C@@]6(OC[C@@]7(OC[C@@]8(OC[C@@]9(OC[C@@]%10(OC[C@@]%11(O[C@H]%12O[C@H](CO)[C@@H](O)[C@H](O)[C@H]%12O)O[C@H](CO)[C@@H](O)[C@@H]%11O)O[C@H](CO)[C@@H](O)[C@@H]%10O)O[C@H](CO)[C@@H](O)[C@@H]9O)O[C@H](CO)[C@@H](O)[C@@H]8O)O[C@H](CO)[C@@H](O)[C@@H]7O)O[C@H](CO)[C@@H](O)[C@@H]6O)O[C@H](CO)[C@@H](O)[C@@H]5O)O[C@H](CO)[C@@H](O)[C@@H]4O)O[C@H](CO)[C@@H](O)[C@@H]3O)O[C@H](CO)[C@@H](O)[C@@H]2O)[C@@H](O)[C@@H]1O FTSSQIKWUOOEGC-RULYVFMPSA-N 0.000 claims description 9
- 229940107187 fructooligosaccharide Drugs 0.000 claims description 9
- 239000001963 growth medium Substances 0.000 claims description 9
- DLRVVLDZNNYCBX-RTPHMHGBSA-N isomaltose Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1OC[C@@H]1[C@@H](O)[C@H](O)[C@@H](O)C(O)O1 DLRVVLDZNNYCBX-RTPHMHGBSA-N 0.000 claims description 9
- 229920001285 xanthan gum Polymers 0.000 claims description 9
- 229940082509 xanthan gum Drugs 0.000 claims description 9
- 235000010493 xanthan gum Nutrition 0.000 claims description 9
- 239000000230 xanthan gum Substances 0.000 claims description 9
- 229920002670 Fructan Polymers 0.000 claims description 8
- 229910000403 monosodium phosphate Inorganic materials 0.000 claims description 8
- 235000019799 monosodium phosphate Nutrition 0.000 claims description 8
- 229910052757 nitrogen Inorganic materials 0.000 claims description 8
- 230000002829 reductive effect Effects 0.000 claims description 8
- AJPJDKMHJJGVTQ-UHFFFAOYSA-M sodium dihydrogen phosphate Chemical compound [Na+].OP(O)([O-])=O AJPJDKMHJJGVTQ-UHFFFAOYSA-M 0.000 claims description 8
- 238000005406 washing Methods 0.000 claims description 8
- 230000001580 bacterial effect Effects 0.000 claims description 7
- 238000000034 method Methods 0.000 claims description 7
- LRWZZZWJMFNZIK-UHFFFAOYSA-N 2-chloro-3-methyloxirane Chemical compound CC1OC1Cl LRWZZZWJMFNZIK-UHFFFAOYSA-N 0.000 claims description 6
- 241000186000 Bifidobacterium Species 0.000 claims description 6
- 241000186660 Lactobacillus Species 0.000 claims description 6
- 241000194020 Streptococcus thermophilus Species 0.000 claims description 6
- 229940039696 lactobacillus Drugs 0.000 claims description 6
- 239000010802 sludge Substances 0.000 claims description 6
- 229960000583 acetic acid Drugs 0.000 claims description 5
- 230000003213 activating effect Effects 0.000 claims description 5
- 238000012258 culturing Methods 0.000 claims description 5
- 239000012362 glacial acetic acid Substances 0.000 claims description 5
- 239000006228 supernatant Substances 0.000 claims description 5
- 238000001291 vacuum drying Methods 0.000 claims description 5
- 241000193830 Bacillus <bacterium> Species 0.000 claims description 4
- 241000186429 Propionibacterium Species 0.000 claims description 4
- 241000235342 Saccharomycetes Species 0.000 claims description 4
- 238000007792 addition Methods 0.000 claims description 4
- 239000007864 aqueous solution Substances 0.000 claims description 4
- 230000007935 neutral effect Effects 0.000 claims description 4
- 239000000203 mixture Substances 0.000 claims description 3
- 238000001953 recrystallisation Methods 0.000 claims 1
- 230000004888 barrier function Effects 0.000 abstract description 12
- 230000003020 moisturizing effect Effects 0.000 abstract description 9
- 230000000694 effects Effects 0.000 abstract description 7
- 238000010521 absorption reaction Methods 0.000 abstract description 6
- 239000002537 cosmetic Substances 0.000 abstract description 5
- 239000002131 composite material Substances 0.000 abstract description 4
- 230000007774 longterm Effects 0.000 abstract description 4
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 abstract description 4
- 229920000832 Cutin Polymers 0.000 abstract description 3
- 239000003795 chemical substances by application Substances 0.000 abstract description 3
- 235000013305 food Nutrition 0.000 abstract description 3
- 238000012423 maintenance Methods 0.000 abstract description 3
- 244000045947 parasite Species 0.000 abstract description 3
- 230000008439 repair process Effects 0.000 abstract description 3
- 210000002374 sebum Anatomy 0.000 abstract description 3
- 230000002195 synergetic effect Effects 0.000 abstract description 3
- 230000003387 muscular Effects 0.000 abstract description 2
- 239000000376 reactant Substances 0.000 abstract description 2
- 238000003860 storage Methods 0.000 abstract description 2
- 210000003491 skin Anatomy 0.000 description 43
- 230000000052 comparative effect Effects 0.000 description 15
- 238000012360 testing method Methods 0.000 description 11
- 241000736262 Microbiota Species 0.000 description 10
- XEKOWRVHYACXOJ-UHFFFAOYSA-N Ethyl acetate Chemical compound CCOC(C)=O XEKOWRVHYACXOJ-UHFFFAOYSA-N 0.000 description 9
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 9
- 230000008591 skin barrier function Effects 0.000 description 8
- 244000005700 microbiome Species 0.000 description 7
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 6
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 6
- ATJFFYVFTNAWJD-UHFFFAOYSA-N Tin Chemical compound [Sn] ATJFFYVFTNAWJD-UHFFFAOYSA-N 0.000 description 6
- 238000010438 heat treatment Methods 0.000 description 6
- 239000002904 solvent Substances 0.000 description 6
- 210000000245 forearm Anatomy 0.000 description 5
- 230000001771 impaired effect Effects 0.000 description 5
- BRLQWZUYTZBJKN-UHFFFAOYSA-N Epichlorohydrin Chemical compound ClCC1CO1 BRLQWZUYTZBJKN-UHFFFAOYSA-N 0.000 description 4
- 238000001035 drying Methods 0.000 description 4
- 230000003698 anagen phase Effects 0.000 description 3
- 239000008367 deionised water Substances 0.000 description 3
- 229910021641 deionized water Inorganic materials 0.000 description 3
- 238000002474 experimental method Methods 0.000 description 3
- 230000014759 maintenance of location Effects 0.000 description 3
- 230000000813 microbial effect Effects 0.000 description 3
- 210000003205 muscle Anatomy 0.000 description 3
- 244000005714 skin microbiome Species 0.000 description 3
- 210000000434 stratum corneum Anatomy 0.000 description 3
- 238000001356 surgical procedure Methods 0.000 description 3
- 230000001052 transient effect Effects 0.000 description 3
- 208000002874 Acne Vulgaris Diseases 0.000 description 2
- 206010067484 Adverse reaction Diseases 0.000 description 2
- 241000233866 Fungi Species 0.000 description 2
- 241000238631 Hexapoda Species 0.000 description 2
- 241000700605 Viruses Species 0.000 description 2
- 206010000496 acne Diseases 0.000 description 2
- 239000013543 active substance Substances 0.000 description 2
- 230000006838 adverse reaction Effects 0.000 description 2
- 244000052616 bacterial pathogen Species 0.000 description 2
- 239000012295 chemical reaction liquid Substances 0.000 description 2
- 201000010099 disease Diseases 0.000 description 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 239000000047 product Substances 0.000 description 2
- 238000007142 ring opening reaction Methods 0.000 description 2
- 238000007619 statistical method Methods 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 230000036572 transepidermal water loss Effects 0.000 description 2
- 206010013786 Dry skin Diseases 0.000 description 1
- 229920001202 Inulin Polymers 0.000 description 1
- 239000004909 Moisturizer Substances 0.000 description 1
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 1
- 206010040844 Skin exfoliation Diseases 0.000 description 1
- 206010040904 Skin odour abnormal Diseases 0.000 description 1
- 238000000692 Student's t-test Methods 0.000 description 1
- 238000006959 Williamson synthesis reaction Methods 0.000 description 1
- 230000002378 acidificating effect Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 235000013361 beverage Nutrition 0.000 description 1
- 125000001549 ceramide group Chemical class 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 230000001010 compromised effect Effects 0.000 description 1
- 125000000113 cyclohexyl group Chemical group [H]C1([H])C([H])([H])C([H])([H])C([H])(*)C([H])([H])C1([H])[H] 0.000 description 1
- 230000006378 damage Effects 0.000 description 1
- 230000035618 desquamation Effects 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- AMTWCFIAVKBGOD-UHFFFAOYSA-N dioxosilane;methoxy-dimethyl-trimethylsilyloxysilane Chemical compound O=[Si]=O.CO[Si](C)(C)O[Si](C)(C)C AMTWCFIAVKBGOD-UHFFFAOYSA-N 0.000 description 1
- 239000003651 drinking water Substances 0.000 description 1
- 235000020188 drinking water Nutrition 0.000 description 1
- 230000037336 dry skin Effects 0.000 description 1
- 230000004064 dysfunction Effects 0.000 description 1
- 239000000839 emulsion Substances 0.000 description 1
- 210000002615 epidermis Anatomy 0.000 description 1
- 125000003700 epoxy group Chemical group 0.000 description 1
- 238000011156 evaluation Methods 0.000 description 1
- 230000036074 healthy skin Effects 0.000 description 1
- 230000013632 homeostatic process Effects 0.000 description 1
- 125000002887 hydroxy group Chemical group [H]O* 0.000 description 1
- 230000028993 immune response Effects 0.000 description 1
- 230000002401 inhibitory effect Effects 0.000 description 1
- 230000003993 interaction Effects 0.000 description 1
- 230000002452 interceptive effect Effects 0.000 description 1
- 229940029339 inulin Drugs 0.000 description 1
- JYJIGFIDKWBXDU-MNNPPOADSA-N inulin Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)OC[C@]1(OC[C@]2(OC[C@]3(OC[C@]4(OC[C@]5(OC[C@]6(OC[C@]7(OC[C@]8(OC[C@]9(OC[C@]%10(OC[C@]%11(OC[C@]%12(OC[C@]%13(OC[C@]%14(OC[C@]%15(OC[C@]%16(OC[C@]%17(OC[C@]%18(OC[C@]%19(OC[C@]%20(OC[C@]%21(OC[C@]%22(OC[C@]%23(OC[C@]%24(OC[C@]%25(OC[C@]%26(OC[C@]%27(OC[C@]%28(OC[C@]%29(OC[C@]%30(OC[C@]%31(OC[C@]%32(OC[C@]%33(OC[C@]%34(OC[C@]%35(OC[C@]%36(O[C@@H]%37[C@@H]([C@@H](O)[C@H](O)[C@@H](CO)O%37)O)[C@H]([C@H](O)[C@@H](CO)O%36)O)[C@H]([C@H](O)[C@@H](CO)O%35)O)[C@H]([C@H](O)[C@@H](CO)O%34)O)[C@H]([C@H](O)[C@@H](CO)O%33)O)[C@H]([C@H](O)[C@@H](CO)O%32)O)[C@H]([C@H](O)[C@@H](CO)O%31)O)[C@H]([C@H](O)[C@@H](CO)O%30)O)[C@H]([C@H](O)[C@@H](CO)O%29)O)[C@H]([C@H](O)[C@@H](CO)O%28)O)[C@H]([C@H](O)[C@@H](CO)O%27)O)[C@H]([C@H](O)[C@@H](CO)O%26)O)[C@H]([C@H](O)[C@@H](CO)O%25)O)[C@H]([C@H](O)[C@@H](CO)O%24)O)[C@H]([C@H](O)[C@@H](CO)O%23)O)[C@H]([C@H](O)[C@@H](CO)O%22)O)[C@H]([C@H](O)[C@@H](CO)O%21)O)[C@H]([C@H](O)[C@@H](CO)O%20)O)[C@H]([C@H](O)[C@@H](CO)O%19)O)[C@H]([C@H](O)[C@@H](CO)O%18)O)[C@H]([C@H](O)[C@@H](CO)O%17)O)[C@H]([C@H](O)[C@@H](CO)O%16)O)[C@H]([C@H](O)[C@@H](CO)O%15)O)[C@H]([C@H](O)[C@@H](CO)O%14)O)[C@H]([C@H](O)[C@@H](CO)O%13)O)[C@H]([C@H](O)[C@@H](CO)O%12)O)[C@H]([C@H](O)[C@@H](CO)O%11)O)[C@H]([C@H](O)[C@@H](CO)O%10)O)[C@H]([C@H](O)[C@@H](CO)O9)O)[C@H]([C@H](O)[C@@H](CO)O8)O)[C@H]([C@H](O)[C@@H](CO)O7)O)[C@H]([C@H](O)[C@@H](CO)O6)O)[C@H]([C@H](O)[C@@H](CO)O5)O)[C@H]([C@H](O)[C@@H](CO)O4)O)[C@H]([C@H](O)[C@@H](CO)O3)O)[C@H]([C@H](O)[C@@H](CO)O2)O)[C@@H](O)[C@H](O)[C@@H](CO)O1 JYJIGFIDKWBXDU-MNNPPOADSA-N 0.000 description 1
- 230000002147 killing effect Effects 0.000 description 1
- 244000005706 microflora Species 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000001333 moisturizer Effects 0.000 description 1
- 210000000056 organ Anatomy 0.000 description 1
- 150000003904 phospholipids Chemical class 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 230000001846 repelling effect Effects 0.000 description 1
- 125000000467 secondary amino group Chemical class [H]N([*:1])[*:2] 0.000 description 1
- 229940083037 simethicone Drugs 0.000 description 1
- 230000037380 skin damage Effects 0.000 description 1
- 208000017520 skin disease Diseases 0.000 description 1
- 206010040882 skin lesion Diseases 0.000 description 1
- 231100000444 skin lesion Toxicity 0.000 description 1
- 230000005808 skin problem Effects 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 238000012353 t test Methods 0.000 description 1
- 238000010998 test method Methods 0.000 description 1
- 230000037303 wrinkles Effects 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/99—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from microorganisms other than algae or fungi, e.g. protozoa or bacteria
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/49—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing heterocyclic compounds
- A61K8/4906—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing heterocyclic compounds with one nitrogen as the only hetero atom
- A61K8/4913—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing heterocyclic compounds with one nitrogen as the only hetero atom having five membered rings, e.g. pyrrolidone carboxylic acid
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/55—Phosphorus compounds
- A61K8/553—Phospholipids, e.g. lecithin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/60—Sugars; Derivatives thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/68—Sphingolipids, e.g. ceramides, cerebrosides, gangliosides
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/72—Cosmetics or similar toiletry preparations characterised by the composition containing organic macromolecular compounds
- A61K8/73—Polysaccharides
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/72—Cosmetics or similar toiletry preparations characterised by the composition containing organic macromolecular compounds
- A61K8/73—Polysaccharides
- A61K8/735—Mucopolysaccharides, e.g. hyaluronic acid; Derivatives thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/72—Cosmetics or similar toiletry preparations characterised by the composition containing organic macromolecular compounds
- A61K8/84—Cosmetics or similar toiletry preparations characterised by the composition containing organic macromolecular compounds obtained by reactions otherwise than those involving only carbon-carbon unsaturated bonds
- A61K8/89—Polysiloxanes
- A61K8/891—Polysiloxanes saturated, e.g. dimethicone, phenyl trimethicone, C24-C28 methicone or stearyl dimethicone
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/40—Chemical, physico-chemical or functional or structural properties of particular ingredients
- A61K2800/59—Mixtures
- A61K2800/592—Mixtures of compounds complementing their respective functions
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Veterinary Medicine (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Birds (AREA)
- Epidemiology (AREA)
- Molecular Biology (AREA)
- Biophysics (AREA)
- Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Dermatology (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Abstract
The invention discloses a probiotic compound for promoting microecological balance and a preparation method thereof, belonging to the technical field of cosmetics. And the probiotic compound comprises probiotics, prebiotics, humectant and water; the humectant comprises ceramide, hyaluronic acid and phosphate ester derivatives; the phosphate derivative is a reactant of phosphate, pyrrolidone and polydimethylsiloxane oil. In addition, the prebiotics are introduced and are composite prebiotics, so that sufficient food is provided for the probiotics, the long-term effective and stable storage of the probiotics is promoted, and the restoration effect of the probiotics on the skin is promoted; meanwhile, the moisturizing agent introduced with the ceramide, the hyaluronic acid and the phosphate derivative has excellent water absorption, moisturizing and film forming effects, promotes cutin to keep moist, expels parasites in skin, and has a synergistic effect with probiotics to form a film maintenance barrier and promote sebum to repair the muscular basal barrier.
Description
Technical Field
The invention belongs to the technical field of cosmetics, and particularly relates to a probiotic compound for promoting micro-ecological balance and a preparation method thereof.
Background
The skin is the largest organ of the human body, and is colonized by billions of microorganisms (bacteria, yeast, fungi, viruses, etc.) collectively called "microbiota" or "microflora". The term "skin microbiome" refers to all these microorganisms, their genomes and their interactions with the environment. Among these microbial flora, there can be divided into a resident flora and a transient flora. The resident flora consists of commensal bacteria, i.e. survive on the host without causing any damage. The transient flora is mainly composed of harmless fungi, viruses and bacteria, and is called saprophytic bacteria. The flora is not permanent and will vary throughout the day depending on the activity being performed and the changes in the surrounding conditions and the degree of exposure of the individual to these conditions. These microorganisms are essential to life. In addition to their role in generating body odour, they are also closely associated with maintaining healthy skin. Under normal conditions, i.e. when the hygiene conditions are good and when the resident flora, immune response and barrier function of the skin are intact, the resident microorganisms and transient microorganisms do not cause diseases or dysfunctions. The skin microbiota is thus able to act as a barrier and protect its host. When the skin microbiota is unbalanced, the function of the muscle bottom barrier can be damaged and lost, so that harmful bacteria grow, and the skin is easy to suffer from diseases such as oil, acne and the like.
However, the use of skin care and cosmetic products, which are currently popular, disrupts the balance of skin microflora. Application of, for example, foundations interferes with the balance of skin microbiota, particularly by interfering with bacterial diversity. The invisible cosmetic film on skin from skin care bottle can temporarily change the water-fat balance of skin and cause desquamation, which can destroy the balance of skin microflora.
Therefore, there is a need to develop a probiotic compound that promotes microbial homeostasis, promotes the rapid restoration of the balance of skin microbiota, and promotes the rapid restoration of the fundal barrier function.
Disclosure of Invention
The invention aims to provide a probiotic compound for promoting microecological balance and a preparation method thereof, so as to provide the probiotic compound which has a strong moisturizing effect, promotes the water-fat balance of skin, restores the balance of skin microbial flora, promotes the rapid recovery of the function of the muscular basal barrier, avoids skin diseases, quickly relieves the skin problems and protects the skin.
The technical problems to be solved by the invention are as follows: solves the problem of disrupting the balance of skin microbiota due to the use of current skin care and cosmetic products.
The purpose of the invention can be realized by the following technical scheme:
a probiotic compound for promoting microecological balance, comprising: probiotics, prebiotics, a humectant and water.
Further, the probiotic compound comprises the following components in percentage by weight of the total compound: 25-65% of probiotics, 2-7% of prebiotics, 1.5-6.5% of humectant and the balance of water.
Further, the complex is in a liquid state, solution or emulsion, and can be sprayed for use.
Further, the probiotic is a live or inactivated microorganism, which is used to increase the diversity of bacteria in the skin microbiota and promote the ecological balance of the skin microbiota.
Further, the probiotics are selected from one or more of the following bacteria in any ratio: lactobacillus, propionibacterium, bacillus, saccharomycete, bifidobacterium and streptococcus thermophilus.
Furthermore, the prebiotics are formed by mixing fructo-oligosaccharide, isomaltooligosaccharide, xanthan gum and inulin-type fructan according to the mass ratio of 1-3:1-3:1-3: 1-3. The prebiotics can provide food for probiotics, can regulate probiotics, and simultaneously can generate a large amount of physiological active substances, regulate the pH value of the skin surface, kill pathogenic bacteria, and inhibit the generation and absorption of endogenous harmful substances.
Further, the moisturizer includes ceramide, hyaluronic acid, and phosphate ester derivatives.
Further, the humectant comprises the following components in percentage by weight based on the total weight of the humectant: 15-25% of ceramide, 20-40% of hyaluronic acid and the balance of phosphate derivatives.
Furthermore, the phosphate derivative is a reactant of phosphate, pyrrolidone and polydimethylsiloxane, has the water absorption and water retention characteristics of the pyrrolidone and phospholipid, and therefore has excellent moisture retention property, and meanwhile, the molecular structure of the phosphate derivative has a polydimethylsiloxane structure, so that the phosphate derivative has a good insect expelling effect, is compounded with ceramide and hyaluronic acid to form a humectant, promotes cutin to keep moist, expels parasites in skin, and has a synergistic effect with probiotics to form a film maintenance barrier and promote sebum repair of the muscle base barrier function.
Further, the molecular structure of the phosphate ester derivative is as follows:
further, the phosphate derivative is prepared by the following steps:
s1, adding epoxy chloropropane, alpha-pyrrolidone and glacial acetic acid into a three-neck flask in sequence, stirring uniformly, carrying out light-shielding treatment by using tinfoil, heating a reaction system to 88 ℃ by using an oil bath kettle under the nitrogen protection state, carrying out reflux reaction for 12h, then carrying out reduced pressure rotary evaporation to remove a solvent to obtain N- (3-chloro-2-hydroxypropyl) -2-pyrrolidone, and utilizing the ring-opening reaction of secondary amine and an epoxy group, wherein the dosage ratio of epoxy chloropropane, alpha-pyrrolidone and glacial acetic acid is 0.1mol:0.21-0.23mol:80-160 mL;
the reaction formula is shown as follows:
s2, adding dihydroxy-terminated polydimethylsiloxane oil and N, N-dimethylformamide into a three-neck flask, uniformly stirring, keeping out of the sun with tinfoil, heating a reaction system to 67 ℃ with an oil bath kettle under the nitrogen protection state, adjusting the pH value of the reaction liquid to 8-9 with a solution of 35% by mass of sodium hydroxide under the stirring state, dropwise adding N- (3-chloro-2-hydroxypropyl) -2-pyrrolidone with a constant-flow dropping funnel, performing reflux reaction for 6 hours, keeping the pH value of the reaction liquid to 8-9, dropwise adding epichlorohydrin with the constant-flow dropping funnel, performing reflux reaction for 4 hours, performing reduced pressure rotary evaporation, washing with deionized water until the washing liquid is neutral, performing vacuum drying to constant weight to obtain an intermediate 2, and performing Williamson synthesis reaction by using dihydroxy-terminated polydimethylsiloxane oil and a chlorohydrocarbon derivative, wherein the dosage ratio of the dihydroxy-terminated polydimethylsiloxane, the N- (3-chloro-2-hydroxypropyl) -2-pyrrolidone, the epichlorohydrin and the N, N-dimethylformamide is 0.01mol:0.011-0.14mol:0.01mol:50-150 mL;
the reaction formula is shown as follows:
s3, adding the intermediate 2 and N, N-dimethylformamide into a three-neck flask, controlling the reaction temperature to be 83 ℃, dropwise adding 30-50% of sodium dihydrogen phosphate aqueous solution by using a constant-pressure dropping funnel at the dropping speed of 1-3 drops/second, adjusting the pH of the reaction solution to be 4-5 by using 10% of hydrochloric acid solution, continuing to react for 5 hours after the dropwise adding is completed, removing the solvent by rotary evaporation, recrystallizing for 2-3 times by using ethyl acetate/ethanol, drying in vacuum to constant weight to obtain a phosphate derivative, and performing ring-opening reaction on cyclohexyl and hydroxyl in sodium dihydrogen phosphate under an acidic condition, wherein the using ratio of the intermediate 2, N-dimethylformamide to sodium dihydrogen phosphate is 0.1mol:50-150mL:0.12-0.13 mol.
The reaction formula is shown as follows:
a preparation method of a probiotic compound for promoting microecological balance comprises the following steps:
A. activating probiotics via slant culture medium, inoculating to fermentation culture medium at 37 deg.C, and culturing to make thallus concentration reach logarithmic growth phase of 0.5-2 × 109CFU/mL to obtain strain fermentation liquor, centrifuging, removing supernatant, and collecting strain mud;
B. the bacterial sludge, the prebiotics, the humectant and the water are stirred and mixed evenly at the speed of 150-200r/min at the temperature of 5-15 ℃, so as to obtain the probiotic compound for promoting the microecological balance.
The invention has the beneficial effects that:
the invention utilizes live or inactivated microorganisms as probiotics as main components of the probiotic compound, and the probiotics are used for increasing the diversity of bacteria in skin microbiota and promoting the ecological balance of the skin microbiota; the mixture of fructo-oligosaccharide, isomaltooligosaccharide, xanthan gum and inulin type fructification is introduced as a prebiotic, the prebiotic is a composite prebiotic, provides sufficient food for the probiotic, can adjust probiotic bacteria, promotes long-term effective and stable storage of the probiotic bacteria, and simultaneously has the functions of generating a large amount of physiological active substances, adjusting the pH value of the surface of skin, killing pathogenic bacteria, inhibiting the generation and absorption of endogenous harmful substances and promoting the restoration of the skin by the probiotic bacteria; meanwhile, the moisturizing agent is introduced, the moisturizing agent is ceramide, hyaluronic acid and phosphate ester derivatives, has excellent water absorption and moisturizing effects, and the hyaluronic acid and the phosphate ester derivatives have excellent film forming effects, promote cutin to keep moist, expel parasites in skin, and play a synergistic effect with probiotics to form a film maintenance barrier and promote sebum to repair the muscle bottom barrier function. The derivative is a combination of phosphate, pyrrolidone and polydimethylsiloxane, and has the water absorption and water retention characteristics of the pyrrolidone and phosphate, so that the derivative has excellent moisture retention property, and meanwhile, the molecular structure of the derivative has a polydimethylsiloxane structure, so that the derivative has a good insect repelling effect.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Example 1
The phosphate derivative is prepared by the following steps:
s1, sequentially adding 0.1mol of epoxy chloropropane, 0.21mol of alpha-pyrrolidone and 80mL of glacial acetic acid into a three-neck flask, uniformly stirring, carrying out light-shielding treatment by using tinfoil, heating a reaction system to 88 ℃ by using an oil bath kettle under the nitrogen protection state, carrying out reflux reaction for 12 hours, and then carrying out reduced pressure rotary evaporation to remove a solvent to obtain N- (3-chloro-2-hydroxypropyl) -2-pyrrolidone;
s2, adding 0.01mol of dihydroxy-terminated polydimethylsiloxane oil and 50mLN, N-dimethylformamide into a three-neck flask, uniformly stirring, carrying out light-shielding treatment by using tinfoil, heating a reaction system to 67 ℃ by using an oil bath kettle in a nitrogen protection state, adjusting the pH value of a reaction solution to 8 by using a solution of 35% by mass of sodium hydroxide in a stirring state, dropwise adding 0.011mol of N- (3-chloro-2-hydroxypropyl) -2-pyrrolidone by using a constant-flow dropping funnel, carrying out reflux reaction for 6 hours, keeping the pH value of the reaction solution to 8, dropwise adding 0.01mol of epichlorohydrin by using the constant-flow dropping funnel, carrying out reflux reaction for 4 hours, carrying out reduced pressure rotary evaporation, washing with deionized water until a washing solution is neutral, and carrying out vacuum drying until the weight is constant, thereby obtaining an intermediate 2;
s3, adding 0.1mol of intermediate 2 and 50mLN, N-dimethylformamide into a three-neck flask, controlling the reaction temperature to 83 ℃, dropwise adding 30% by mass of sodium dihydrogen phosphate aqueous solution by using a constant-pressure dropping funnel at a dropping speed of 1 drop/second, adjusting the pH of the reaction solution to 4 by using 10% by mass of hydrochloric acid solution, continuing to react for 5 hours after complete dropwise addition, removing the solvent by rotary evaporation, recrystallizing for 2 times by using ethyl acetate/ethanol, and drying in vacuum to constant weight to obtain the phosphate derivative, wherein the mole number of the sodium dihydrogen phosphate is 0.12 mol.
Example 2:
the phosphate derivative is prepared by the following steps:
s1, sequentially adding 0.1mol of epoxy chloropropane, 0.23mol of alpha-pyrrolidone and 160mL of glacial acetic acid into a three-neck flask, uniformly stirring, carrying out light-shielding treatment by using tinfoil, heating a reaction system to 88 ℃ by using an oil bath kettle under the nitrogen protection state, carrying out reflux reaction for 12 hours, and then carrying out reduced pressure rotary evaporation to remove a solvent to obtain N- (3-chloro-2-hydroxypropyl) -2-pyrrolidone;
s2, adding 0.01mol of dihydroxy-terminated polydimethylsiloxane oil and 150mLN, N-dimethylformamide into a three-neck flask, uniformly stirring, carrying out light-shielding treatment by using tinfoil, heating a reaction system to 67 ℃ by using an oil bath kettle in a nitrogen protection state, adjusting the pH value of a reaction solution to 8.3 by using a solution of 35% by mass of sodium hydroxide in a stirring state, dropwise adding 0.14mol of N- (3-chloro-2-hydroxypropyl) -2-pyrrolidone by using a constant-current dropping funnel, carrying out reflux reaction for 6h, keeping the pH value of the reaction solution to 8.3, dropwise adding 0.01mol of epichlorohydrin by using the constant-current dropping funnel, carrying out reflux reaction for 4h, carrying out reduced pressure rotary evaporation, washing by using deionized water until a washing solution is neutral, and carrying out vacuum drying until the weight is constant to obtain an intermediate 2;
s3, adding 0.1mol of intermediate 2 and 150mLN, N-dimethylformamide into a three-neck flask, controlling the reaction temperature to 83 ℃, dropwise adding 40% by mass of sodium dihydrogen phosphate aqueous solution by using a constant-pressure dropping funnel at a dropping speed of 1 drop/second, adjusting the pH of the reaction solution to 4.3 by using 10% by mass of hydrochloric acid solution, continuing to react for 5 hours after complete dropwise addition, removing the solvent by rotary evaporation, recrystallizing for 2 times by using ethyl acetate/ethanol, and drying in vacuum to constant weight to obtain the phosphate derivative, wherein the mole number of the sodium dihydrogen phosphate is 0.13 mol.
Example 3:
a probiotic compound for promoting microecological balance comprises the following components in parts by weight: 25% of probiotics, 2% of prebiotics, 1.5% of humectant and the balance of water; wherein the probiotic is lactobacillus; the prebiotics are formed by mixing fructo-oligosaccharide, isomaltooligosaccharide, xanthan gum and inulin-type fructan according to the mass ratio of 1:1:1: 1; the humectant comprises the following components in percentage by weight based on the total weight of the humectant: 15% of ceramide, 20% of hyaluronic acid and the balance of the phosphate derivative prepared in example 1;
the probiotic compound for promoting microecological balance is prepared by the following steps:
A. activating probiotics via slant culture medium, inoculating to fermentation culture medium at 37 deg.C, and culturing to make thallus concentration reach logarithmic phase of 0.5 × 109CFU/mL to obtain strain fermentation liquor, centrifuging, removing supernatant, and collecting strain mud;
B. the bacterial sludge, the prebiotics, the humectant and the water are stirred and mixed evenly at the speed of 150r/min at the temperature of 5 ℃, and the probiotic compound for promoting the micro-ecological balance is obtained.
Example 4:
a probiotic compound for promoting microecological balance comprises the following components in parts by weight: 30% of probiotics, 4% of prebiotics, 4% of humectant and the balance of water; wherein the probiotics are composite bacteria formed by mixing lactobacillus, propionibacterium, bacillus, saccharomycetes, bifidobacterium and streptococcus thermophilus according to the mass ratio of 1:2:1.5:3:1: 2; the prebiotics are formed by mixing fructo-oligosaccharide, isomaltooligosaccharide, xanthan gum and inulin-type fructan according to the mass ratio of 1:1.6:2: 1; the humectant comprises the following components in percentage by weight based on the total weight of the humectant: 20% of ceramide, 30% of hyaluronic acid and the balance of the phosphate derivative prepared in example 2;
the probiotic compound for promoting microecological balance is prepared by the following steps:
A. activating probiotics via slant culture medium, inoculating to fermentation culture medium at 37 deg.C, and culturing to make thallus concentration reach logarithmic growth phase of 1 × 109CFU/mL to obtain strain fermentation liquor, centrifuging, removing supernatant, and collecting strain mud;
B. the bacterial sludge, the prebiotics, the humectant and the water are stirred and mixed evenly at the speed of 200r/min at the temperature of 10 ℃, and the probiotic compound for promoting the micro-ecological balance is obtained.
Example 5:
a probiotic compound for promoting microecological balance comprises the following components in parts by weight: 65% of probiotics, 7% of prebiotics, 6.5% of humectant and the balance of water; wherein the probiotics are selected from bifidobacteria and streptococcus thermophilus which are mixed according to the mass ratio of 1: 1; the prebiotics are formed by mixing fructo-oligosaccharide, isomaltooligosaccharide, xanthan gum and inulin-type fructan according to the mass ratio of 3:1:3: 2; the humectant comprises the following components in percentage by weight based on the total weight of the humectant: 25% of ceramide, 40% of hyaluronic acid and the balance of the phosphate derivative prepared in example 1;
the probiotic compound for promoting microecological balance is prepared by the following steps:
A. activating probiotics via slant culture medium, inoculating to fermentation culture medium at 37 deg.C, and culturing to make thallus concentration reach logarithmic growth phase 2 × 109CFU/mL to obtain strain fermentation liquor, centrifuging, removing supernatant, and collecting strain mud;
B. the bacterial sludge, the prebiotics, the humectant and the water are stirred and mixed evenly at the speed of 200r/min at the temperature of 15 ℃, and the probiotic compound for promoting the micro-ecological balance is obtained.
Comparative example 1:
a probiotic compound for promoting microecological balance comprises the following components in parts by weight: 25% of probiotics, 2% of prebiotics, 1.5% of humectant and the balance of water; wherein the probiotic is lactobacillus; the prebiotics are formed by mixing fructo-oligosaccharide, isomaltooligosaccharide, xanthan gum and inulin-type fructan according to the mass ratio of 1:1:1: 1; the humectant comprises the following components in percentage by weight based on the total weight of the humectant: 15% of ceramide, 20% of hyaluronic acid and the balance of simethicone;
the probiotic compound for promoting microecological balance is prepared by the following steps: refer to the procedure for preparation in example 1.
Comparative example 2:
a probiotic compound for promoting microecological balance comprises the following components in parts by weight: 30% of probiotics, 4% of humectant and the balance of water; wherein the probiotics are composite bacteria formed by mixing lactobacillus, propionibacterium, bacillus, saccharomycetes, bifidobacterium and streptococcus thermophilus according to the mass ratio of 1:2:1.5:3:1: 2; the prebiotics are formed by mixing fructo-oligosaccharide, isomaltooligosaccharide, xanthan gum and inulin-type fructan according to the mass ratio of 1:1.6:2: 1; the humectant comprises the following components in percentage by weight based on the total weight of the humectant: 20% of ceramide, 30% of hyaluronic acid and the balance of the phosphate derivative prepared in example 2;
the probiotic compound for promoting microecological balance is prepared by the following steps: refer to the procedure for preparation in example 4.
Comparative example 3:
a probiotic compound for promoting microecological balance comprises the following components in parts by weight: 65% of probiotics, 7% of prebiotics and the balance of water; wherein the probiotics are selected from bifidobacteria and streptococcus thermophilus which are mixed according to the mass ratio of 1: 1; the prebiotics are formed by mixing fructo-oligosaccharide, isomaltooligosaccharide, xanthan gum and inulin-type fructan according to the mass ratio of 3:1:3: 2;
the probiotic compound for promoting microecological balance is prepared by the following steps: refer to the procedure for preparation in example 5.
Example 6:
the probiotic complexes obtained in examples 3 to 5 and comparative examples 1 to 3 were tested according to the following test methods:
first, experiment for recovering function of muscle-bottom barrier
(1) The probiotic compound obtained in examples 3-5 and comparative examples 1-3 was applied, diluted 10 times with water, and sprayed on the face of the subject;
(2) the subject: 40 men, 100 women, 20-55 years of age, and a mean age (30 ± 14) of age, wherein 14 people are over-cleansed resulting in an impaired skin barrier, 28 people are under-exfoliated resulting in an impaired skin barrier, 32 people are under-exposed to laser wrinkle removal surgery resulting in an impaired skin barrier, 32 people are under-exposed to laser speckle removal surgery resulting in an impaired skin barrier, and 34 people are under-exposed to laser acne removal surgery resulting in an impaired skin barrier. All subjects were randomized into 7 groups, one control and 6 experimental.
(3) The experimental method comprises the following steps: the experimental groups used the samples prepared in examples 1-3 and comparative examples 1-3; control 1 was not smeared with any sample. After six groups of skin lesions were cleaned, the test article was used. The composition is administered once daily for 20 days.
(4) And (3) safety evaluation: in the clinical use process, adverse reactions do not appear through careful observation, the adverse reactions are carefully recorded and tracked, and if the patient is found to be uncomfortable, the experiment should be immediately interrupted and measures are actively taken.
(5) Skin barrier function test: the stratum corneum water content and the transepidermal water loss were measured using a skin water tester (VapoMeter) and a transepidermal water loss meter (vapometerttewl) of delf and finland corporation. The skin is cleaned before detection, and the skin enters a test environment 30 minutes in advance to be quietly tested, and non-skin damage parts of cheeks are respectively detected.
(6) The statistical method comprises the following steps: statistical analysis statistical processing was performed using the t-test using SPSS3.0 software.
The statistical results are shown in table 1 below.
TABLE 1
As shown in table 1, the skin had a higher transdermal water loss and a lower stratum corneum water content after the skin barrier had been compromised prior to treatment. After the treatment, the effect of improving the transdermal water loss using the complexes of examples 3 to 5 was superior to that using comparative examples 1 to 3, the increase in the moisture content of the stratum corneum using the complexes of examples 3 to 5 was significantly superior to that using comparative examples 1 to 3, and it was demonstrated that the skin barrier function improving effect of the subjects was superior to that of examples 3 to 5 compared to comparative examples 1 to 3.
Secondly, detecting the skin moisturizing function:
the probiotic compound obtained in examples 3-5 and comparative examples 1-3 was diluted 15 times.
(1) 70 dry skin volunteers (skin moisture content less than 10%) aged 30-50 were selected and randomized into 7 groups of 10 persons each, one of which was blank.
(2) Cleaning the inner sides of the forearms of both hands of the subject uniformly, sitting still in a laboratory, drinking water and beverage without intentional confusion, exposing the forearms, keeping the forearms relaxed, naturally drying for 0.5 hour, and selecting 3cm on the inner sides of the forearms of both hands respectively2The area of (A) serves as a test area and a control areaThe front inner sides of the two arms of the group and the experimental group 1-5 are respectively smeared with 0.2mL of the corresponding test article, the empire state is measured by a skin epidermal layer water content tester at each time point of 1, 2, 4 and 6h after the smearing, and the water content of the tested area of the front inner sides of the two arms of each test subject is recorded.
(3) And taking the average value of the moisture content of the tested areas on the inner sides of the two forearm of the testee as the moisture content test result of the skin of the testee, and taking the average value of the moisture content test results of the skins of the testees of all groups as the moisture content test result of the skin of the testee of the group. The long-term moisturizing effect of the invention is judged by comparing the difference of the skin moisture content of each group before and after being smeared with the test article and the difference of the skin moisture content of the blank control group. And the results of the moisture content test of the skin are shown in table 2.
TABLE 2
1h | 2h | 4h | 6h | |
Blank group | 28.3 | 28.5 | 28.9 | 28.7 |
Example 3 | 55.3 | 49.4 | 45.7 | 42.5 |
Example 4 | 54.6 | 48.9 | 44.4 | 40.8 |
Example 5 | 56.1 | 50.3 | 46.0 | 41.3 |
Comparative example 1 | 45.9 | 37.2 | 29.8 | 28.3 |
Comparative example 2 | 46.2 | 37.4 | 33.0 | 28.1 |
Comparative example 3 | 39.2 | 30.3 | 28.5 | 28.3 |
As shown in table 2, the moisture content of the skin of the subjects in the blank group hardly changed within 6h, after 6h, the moisture content of the skin of the subjects using examples 3 to 5 was significantly higher than that of the skin of the subjects using comparative examples 1 to 3, and within 6h, the decrease in the moisture content of the skin of the subjects using comparative example 3 was significant, and the moisture content retention rate of the skin of the subjects using examples 3 to 5 was the best, indicating that the probiotic compound of the present invention has a significant long-term moisturizing function on the skin.
In the description herein, references to the description of "one embodiment," "an example," "a specific example" or the like are intended to mean that a particular feature, structure, material, or characteristic described in connection with the embodiment or example is included in at least one embodiment or example of the invention. In this specification, the schematic representations of the terms used above do not necessarily refer to the same embodiment or example. Furthermore, the particular features, structures, materials, or characteristics described may be combined in any suitable manner in any one or more embodiments or examples.
The foregoing is illustrative and explanatory only and is not intended to be exhaustive or to limit the invention to the precise embodiments described, and various modifications, additions, and substitutions may be made by those skilled in the art without departing from the scope of the invention or exceeding the scope of the claims.
Claims (7)
1. A probiotic compound for promoting microecological balance, characterized by: the method comprises the following steps: probiotics, prebiotics, humectants, and water;
the humectant comprises ceramide, hyaluronic acid and phosphate ester derivatives;
the phosphate derivative is prepared by the following steps:
x1, mixing dihydroxy-terminated polydimethylsiloxane oil and N, N-dimethylformamide, adjusting the pH value of a reaction solution to 8-9 under the protection of light and nitrogen at 67 ℃, dropwise adding N- (3-chloro-2-hydroxypropyl) -2-pyrrolidone, performing reflux reaction for 6 hours, continuously keeping the pH value of the reaction solution to 8-9, dropwise adding epoxy chloropropane, performing reflux reaction for 4 hours, performing reduced pressure rotary evaporation, washing until a washing solution is neutral, and performing vacuum drying to obtain an intermediate 2;
and X2, mixing the intermediate 2 with N, N-dimethylformamide, controlling the reaction temperature to 83 ℃, dropwise adding a sodium dihydrogen phosphate aqueous solution, keeping the pH of the reaction solution at 4-5, continuously reacting for 5h after complete dropwise addition, and performing rotary evaporation, recrystallization and vacuum drying to obtain the phosphate derivative.
2. A probiotic compound for promoting microecological balance according to claim 1, characterized in that: the compound comprises the following components in percentage by weight of the total compound: 25-65% of probiotics, 2-7% of prebiotics, 1.5-6.5% of humectant and the balance of water.
3. A probiotic compound for promoting microecological balance according to claim 1, characterized in that: the probiotics are selected from one or more of the following bacteria in any ratio: lactobacillus, propionibacterium, bacillus, saccharomycete, bifidobacterium and streptococcus thermophilus.
4. A probiotic compound for promoting microecological balance according to claim 2, characterized in that: the prebiotics are formed by mixing fructo-oligosaccharide, isomaltooligosaccharide, xanthan gum and inulin-type fructan according to the mass ratio of 1-3:1-3: 1-3.
5. A probiotic compound for promoting microecological balance according to claim 1, characterized in that: the N- (3-chloro-2-hydroxypropyl) -2-pyrrolidone is prepared by the following steps:
mixing epoxy chloropropane, alpha-pyrrolidone and glacial acetic acid, carrying out reflux reaction at 88 ℃ for 12h under the protection of light and nitrogen, and then carrying out rotary evaporation under reduced pressure to obtain the N- (3-chloro-2-hydroxypropyl) -2-pyrrolidone.
6. A probiotic compound for promoting microecological balance according to claim 1, characterized in that: the humectant comprises the following components in percentage by weight based on the total weight of the humectant: 15-25% of ceramide, 20-40% of hyaluronic acid and the balance of phosphate derivatives.
7. The method for preparing probiotic compound for promoting microecological balance according to claim 1, wherein the probiotic compound comprises the following components: the method comprises the following steps:
A. activating probiotics, inoculating the activated probiotics into a fermentation culture medium, culturing at 37 ℃ to obtain strain fermentation liquor, centrifuging, removing supernatant, and collecting bacterial sludge;
B. and (3) stirring and mixing the bacterial sludge, the prebiotics, the humectant and the water at the temperature of 5-15 ℃ until the mixture is uniform, thus obtaining the probiotic compound for promoting the microecological balance.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN202110866279.1A CN113546033A (en) | 2021-07-29 | 2021-07-29 | Probiotic compound for promoting microecological balance and preparation method thereof |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN202110866279.1A CN113546033A (en) | 2021-07-29 | 2021-07-29 | Probiotic compound for promoting microecological balance and preparation method thereof |
Publications (1)
Publication Number | Publication Date |
---|---|
CN113546033A true CN113546033A (en) | 2021-10-26 |
Family
ID=78104861
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN202110866279.1A Pending CN113546033A (en) | 2021-07-29 | 2021-07-29 | Probiotic compound for promoting microecological balance and preparation method thereof |
Country Status (1)
Country | Link |
---|---|
CN (1) | CN113546033A (en) |
Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5070171A (en) * | 1990-06-27 | 1991-12-03 | Siltech Inc. | Phosphated silicone polymers |
CN1950460A (en) * | 2004-03-05 | 2007-04-18 | 庄臣及庄臣视力保护公司 | Wettable hydrogels comprising acyclic polyamides |
CN101437578A (en) * | 2006-05-01 | 2009-05-20 | 高露洁-棕榄公司 | Oral compositions comprising siloxane polymers |
CN111265470A (en) * | 2020-03-30 | 2020-06-12 | 西安润玉医疗科技有限公司 | Microecological restoration composition and preparation method thereof |
-
2021
- 2021-07-29 CN CN202110866279.1A patent/CN113546033A/en active Pending
Patent Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5070171A (en) * | 1990-06-27 | 1991-12-03 | Siltech Inc. | Phosphated silicone polymers |
CN1950460A (en) * | 2004-03-05 | 2007-04-18 | 庄臣及庄臣视力保护公司 | Wettable hydrogels comprising acyclic polyamides |
CN101437578A (en) * | 2006-05-01 | 2009-05-20 | 高露洁-棕榄公司 | Oral compositions comprising siloxane polymers |
CN111265470A (en) * | 2020-03-30 | 2020-06-12 | 西安润玉医疗科技有限公司 | Microecological restoration composition and preparation method thereof |
Non-Patent Citations (2)
Title |
---|
国家药品监督管理局: "《化妆品安全技术规范》", 1 December 2016 * |
康白: "《微生态学现代理论与应用 康白教授的微生态观》", 31 January 2013, 上海科学技术出版社 * |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
CN112980892B (en) | Composite probiotic fermented product with skin care effect and preparation and application thereof | |
McGinley et al. | Regional variations in density of cutaneous propionibacteria: correlation of Propionibacterium acnes populations with sebaceous secretion | |
KR101398347B1 (en) | Methods and means for protecting the skin against pathogenic microorganisms | |
US20090035294A1 (en) | Lipopolysaccharide fractions of vitreoscilla filiformis useful for stimulating the synthesis of anti-microbial peptides of the skin | |
CN109789173A (en) | Nano vesicle and application thereof from bacillus bacterium | |
CN105473137B (en) | For prevent or treat menopause symptom, include the composition of the extract of soybean containing coumestrol as active constituent | |
CN108697743A (en) | For treating the composition for infecting the especially lactic acid bacteria of acne caused by propionibacterium acnes | |
KR101955111B1 (en) | External composition for soothing effect on the skin comprising an extract of fermented wheat germ | |
KR101929658B1 (en) | Skin external agent for skin whitening comprising an extract of fermented wheat germ | |
CN112870151B (en) | Skin barrier repair composition containing sodium polyglutamate and application thereof | |
CN114728032A (en) | A cosmetic composition containing Taraxacum officinale extract for improving skin | |
US20190247295A1 (en) | Use of lactobacillus plantarum gmnl-6 composition for skin care | |
CN107137628A (en) | Hyaluronidase inhibitor and its application from symbiotic fermentation product | |
JP4000078B2 (en) | Skin moisturizer | |
KR102270709B1 (en) | Cosmetic composition for skin improvement containing complex ceramide and natural extracts | |
CN108125810A (en) | A kind of compound moisturizing remediation composition and its preparation method and application | |
CN111904909B (en) | Dandruff removing scalp essence containing rose fermentation liquor and preparation method thereof | |
CN114569536A (en) | A lotion for improving microecology and physiological state of aged skin | |
CN114736941A (en) | Sodium hyaluronate fermentation product, skin external preparation containing sodium hyaluronate fermentation product, and preparation method and application of sodium hyaluronate fermentation product | |
CN112107512B (en) | Scalp essence containing ganoderma lucidum sporophore spore powder fermentation liquid and preparation method thereof | |
CN116077415B (en) | Ternary probiotic factor composition for regulating skin microecological balance | |
CN107106479B (en) | Composition for improving the cellulite appearance of the skin | |
JP6976843B2 (en) | Skin condition improving agent | |
CN113546033A (en) | Probiotic compound for promoting microecological balance and preparation method thereof | |
CN114984063B (en) | Skin external composition and functional food composition |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
PB01 | Publication | ||
PB01 | Publication | ||
SE01 | Entry into force of request for substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
RJ01 | Rejection of invention patent application after publication |
Application publication date: 20211026 |
|
RJ01 | Rejection of invention patent application after publication |