CN113527497A - 抗Trop2纳米抗体及其应用 - Google Patents
抗Trop2纳米抗体及其应用 Download PDFInfo
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- CN113527497A CN113527497A CN202110179743.XA CN202110179743A CN113527497A CN 113527497 A CN113527497 A CN 113527497A CN 202110179743 A CN202110179743 A CN 202110179743A CN 113527497 A CN113527497 A CN 113527497A
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- 108700012359 toxins Proteins 0.000 description 1
- 238000013518 transcription Methods 0.000 description 1
- 230000035897 transcription Effects 0.000 description 1
- 230000001131 transforming effect Effects 0.000 description 1
- 230000010474 transient expression Effects 0.000 description 1
- 108091005703 transmembrane proteins Proteins 0.000 description 1
- 102000035160 transmembrane proteins Human genes 0.000 description 1
- QORWJWZARLRLPR-UHFFFAOYSA-H tricalcium bis(phosphate) Chemical compound [Ca+2].[Ca+2].[Ca+2].[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O QORWJWZARLRLPR-UHFFFAOYSA-H 0.000 description 1
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- C07K16/28—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
- C07K16/30—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants from tumour cells
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
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Abstract
Description
最初的残基 | 代表性的取代 | 优选的取代 |
Ala(A) | Val;Leu;Ile | Val |
Arg(R) | Lys;Gln;Asn | Lys |
Asn(N) | Gln;His;Lys;Arg | Gln |
Asp(D) | Glu | Glu |
Cys(C) | Ser | Ser |
Gln(Q) | Asn | Asn |
Glu(E) | Asp | Asp |
Gly(G) | Pro;Ala | Ala |
His(H) | Asn;Gln;Lys;Arg | Arg |
Ile(I) | Leu;Val;Met;Ala;Phe | Leu |
Leu(L) | Ile;Val;Met;Ala;Phe | Ile |
Lys(K) | Arg;Gln;Asn | Arg |
Met(M) | Leu;Phe;Ile | Leu |
Phe(F) | Leu;Val;Ile;Ala;Tyr | Leu |
Pro(P) | Ala | Ala |
Ser(S) | Thr | Thr |
Thr(T) | Ser | Ser |
Trp(W) | Tyr;Phe | Tyr |
Tyr(Y) | Trp;Phe;Thr;Ser | Phe |
Val(V) | Ile;Leu;Met;Phe;Ala | Leu |
抗体编号 | 氨基酸序列编号 | 核苷酸序列编号 |
Nb1-70 | SEQ ID NO:8 | SEQ ID NO:9 |
Nb1-78 | SEQ ID NO:16 | SEQ ID NO:17 |
Nb1-81 | SEQ ID NO:23 | SEQ ID NO:24 |
Nb2-22 | SEQ ID NO:31 | SEQ ID NO:32 |
Nb2-38 | SEQ ID NO:39 | SEQ ID NO:40 |
Nb2-59 | SEQ ID NO:47 | SEQ ID NO:48 |
Nb6-24 | SEQ ID NO:54 | SEQ ID NO:55 |
抗体编号 | 氨基酸序列编号 | 核苷酸序列编号 |
HuNb1-70 | SEQ ID NO:60 | SEQ ID NO:61 |
HuNb1-78 | SEQ ID NO:65 | SEQ ID NO:66 |
HuNb1-81 | SEQ ID NO:68 | SEQ ID NO:69 |
HuNb2-22 | SEQ ID NO:72 | SEQ ID NO:73 |
HuNb2-38 | SEQ ID NO:75 | SEQ ID NO:76 |
HuNb2-59 | SEQ ID NO:78 | SEQ ID NO:79 |
HuNb6-24 | SEQ ID NO:82 | SEQ ID NO:83 |
Claims (10)
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Cited By (4)
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CN114805582A (zh) * | 2022-06-29 | 2022-07-29 | 上海恒润达生生物科技股份有限公司 | 抗Trop2纳米抗体及其用途 |
CN115232212A (zh) * | 2022-05-27 | 2022-10-25 | 南开大学 | 一种trop-2特异性纳米抗体及其应用 |
CN115873104A (zh) * | 2022-08-09 | 2023-03-31 | 华南农业大学 | 针对h7亚型禽流感病毒的纳米抗体m124及其应用 |
WO2024051383A1 (zh) * | 2023-07-28 | 2024-03-14 | 上海洛启生物医药技术有限公司 | 抗Trop2抗体、包含所述抗体的缀合物及其应用 |
Families Citing this family (2)
Publication number | Priority date | Publication date | Assignee | Title |
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CN112321715B (zh) * | 2020-11-03 | 2022-05-10 | 博奥信生物技术(南京)有限公司 | 抗trop2纳米抗体及其制备方法和应用 |
IL311673A (en) * | 2021-09-29 | 2024-05-01 | Kisoji Biotechnology Inc | Binding agents targeting TROP2-expressing tumor cells |
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Cited By (6)
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CN115232212A (zh) * | 2022-05-27 | 2022-10-25 | 南开大学 | 一种trop-2特异性纳米抗体及其应用 |
CN115232212B (zh) * | 2022-05-27 | 2024-02-23 | 南开大学 | 一种trop-2特异性纳米抗体及其应用 |
CN114805582A (zh) * | 2022-06-29 | 2022-07-29 | 上海恒润达生生物科技股份有限公司 | 抗Trop2纳米抗体及其用途 |
CN115873104A (zh) * | 2022-08-09 | 2023-03-31 | 华南农业大学 | 针对h7亚型禽流感病毒的纳米抗体m124及其应用 |
CN115873104B (zh) * | 2022-08-09 | 2023-07-14 | 华南农业大学 | 针对h7亚型禽流感病毒的纳米抗体m124及其应用 |
WO2024051383A1 (zh) * | 2023-07-28 | 2024-03-14 | 上海洛启生物医药技术有限公司 | 抗Trop2抗体、包含所述抗体的缀合物及其应用 |
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