CN113514592A - Movable electrospray ion source accurate positioning device linked with chromatographic valve - Google Patents
Movable electrospray ion source accurate positioning device linked with chromatographic valve Download PDFInfo
- Publication number
- CN113514592A CN113514592A CN202010274334.3A CN202010274334A CN113514592A CN 113514592 A CN113514592 A CN 113514592A CN 202010274334 A CN202010274334 A CN 202010274334A CN 113514592 A CN113514592 A CN 113514592A
- Authority
- CN
- China
- Prior art keywords
- mass spectrum
- valve
- tray
- chromatographic column
- injection port
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Images
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/88—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86
Landscapes
- Physics & Mathematics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Other Investigation Or Analysis Of Materials By Electrical Means (AREA)
Abstract
The embodiment of the invention provides a mobile electrospray ion source accurate positioning device linked with a chromatographic valve, which is used for connecting a liquid chromatograph and a mass spectrometer, wherein the liquid chromatograph is connected with the chromatographic valve, a valve head of the chromatographic valve is provided with a plurality of interfaces communicated with the chromatographic valve, and the accurate positioning device comprises: the connecting bracket is arranged on the mass spectrum panel; the carrying tray is arranged on the connecting bracket; a controller; the sensor is connected with the controller and used for sensing the rotating position information of the valve head and sending the rotating position information to the controller; the driving mechanism is connected with the controller; and the controller determines a capillary chromatographic column connected to the liquid chromatograph according to the received rotation position information and enables the capillary chromatographic column to be opposite to the mass spectrum ion injection port. The invention utilizes the linkage of the inductor and the chromatographic valve, can realize the accurate positioning of the capillary chromatographic column, helps to realize the full-automatic on-line switching and selection of the capillary chromatographic column, expands the performance and the application range of the instrument, and improves the automation level and the use efficiency of the instrument.
Description
Technical Field
The invention belongs to the field of mass spectrometry, particularly relates to a precise positioning device for an ion source device, and particularly relates to a movable electrospray ion source precise positioning device linked with a chromatographic valve.
Background
A mass spectrometry Ion source (Ion source) is a device that ionizes neutral atoms or molecules and extracts an Ion beam stream therefrom. Electrospray (ESI) ion sources are currently the most commonly used link devices for liquid chromatography and mass spectrometry, and can be used for studying thermally unstable and more polar compounds, and especially play an important role in the field of protein analysis.
At present, the existing electrospray ion source is generally arranged at the front end of an ion injection opening of a mass spectrum through a fixed support, and the ionization process of a substance to be detected is realized by applying high voltage to an electrospray spray needle or the substance to be detected. The relative position of the electric spray needle outlet and the mass spectrum ion injection port can only be finely adjusted manually in a small range by the fixing support, and linkage matching with a front-end chromatographic column control selection valve cannot be realized. Therefore, in order to realize the online real-time switching function of the chromatographic analysis column, an additional chromatographic valve needs to be purchased and assembled at the rear end of the chromatographic column, which can significantly increase the system dead volume, reduce the chromatographic separation efficiency and limit the application. Even for a part of direct injection capillary chromatographic columns, the chromatographic columns can be installed only by stopping the machine and manually switching the machine. This not only makes the operation and maintenance inconvenient, but also restricts the performance and use of the LC-MS apparatus.
Disclosure of Invention
In view of the above problems in the prior art, an embodiment of the present invention provides a mobile electrospray ion source precision positioning device with linked chromatography valves, which can realize precision adjustment of positions of a capillary chromatography column and a mass spectrometry ion injection port, and can switch and select the capillary chromatography column on line.
In order to solve the above problems, the embodiment of the present invention provides the following technical solutions: the utility model provides a accurate positioner of portable electrospray ion source of chromatography valve linkage for the connection of liquid chromatograph and mass spectrograph, the liquid chromatograph is connected with the chromatography valve, the valve head of chromatography valve has selectively with a plurality of interfaces of chromatography valve intercommunication, the accurate positioner of portable electrospray ion source of chromatography valve linkage includes:
the connecting bracket is detachably arranged on a mass spectrum panel of the mass spectrometer;
the carrying tray is arranged on the connecting bracket;
a controller;
the sensor is connected with the controller and used for sensing the rotating position information of the valve head and sending the rotating position information to the controller;
a drive mechanism connected to the controller; wherein the content of the first and second substances,
the controller determines an interface of the valve head communicated with the liquid chromatograph according to the received rotation position information so as to determine a capillary chromatographic column connected to the liquid chromatograph, and controls the driving mechanism to enable the carrying tray to drive the capillary chromatographic column to move on the connecting support, so that an outlet of the capillary chromatographic column is opposite to a mass spectrum ion injection port on the mass spectrum panel.
In some embodiments, the sensor includes a plurality of magnetic proximity switches corresponding to the interfaces of the valve head in a one-to-one manner, and when a first interface is conducted with the liquid chromatograph, a first magnetic proximity switch corresponding to the first interface is closed to generate a corresponding position signal;
the controller determines a first capillary chromatographic column communicated with the liquid chromatograph according to the acquired position signal generated by the first magnetic proximity switch, and controls the driving mechanism to drive the carrying tray to move, so that an outlet of the first capillary chromatographic column is opposite to the mass spectrum ion injection port on the mass spectrum panel.
In some embodiments, the apparatus further comprises a horizontal base fixed on the stage, and the carrier tray is movably disposed on the horizontal base.
In some embodiments, a plurality of positioning metal tracking bars are arranged on the transverse base at intervals along a horizontal direction perpendicular to the orientation direction of the mass spectrum ion injection port, and the positioning metal tracking bars are connected with the magnetic proximity switches one by one;
the bottom of the carrying tray is provided with a contact which can be conducted with the positioning metal tracing strip;
the driving mechanism comprises a driving motor, the driving motor is arranged in the transverse base and used for driving the carrying tray to move on the transverse base along the arrangement direction of the positioning metal tracing strips, when the first magnetic approach switch is closed, the contact on the bottom of the carrying tray is touched by the first positioning metal tracing strips to generate the position signal, and meanwhile, the driving motor stops rotating.
In some embodiments, limit switches are respectively disposed on two sides of the alignment direction of the positioning metal track bars, so that when the driving motor drives the carrying tray to move to trigger the limit switches, the driving motor drives the carrying tray to move reversely.
In some embodiments, the connecting bracket comprises:
the mounting table is provided with an avoiding space for avoiding the mass spectrum ion injection port so that the mass spectrum ion injection port is directly opposite to the carrying tray through the avoiding space, and the mounting table is detachably connected with the mass spectrum panel;
a guide rail fixed to the mount table;
the object stage is movably arranged on the guide rail, and the object tray is arranged on the object stage.
In some embodiments, be equipped with on the mount table can be relative the flat cylindricality of mount table pivoted pintle, be equipped with on the mass spectrum panel be used for with pintle complex locking groove, the notch size of locking groove is less than the groove cavity size of locking groove, so that the pintle rotate to its narrow side with the notch is relative can enter into locking inslot, just the pintle rotate to its wide side with the notch is relative inject in locking inslot, so that the mount table is fixed in on the mass spectrum panel.
In some embodiments, the mobile electrospray ion source precision positioning device linked with the chromatographic valve further comprises a height adjusting mechanism, and the height adjusting mechanism is arranged on the carrying tray and used for adjusting the height of the carrying tray, so that the position of the capillary chromatographic column on the carrying tray in the vertical direction corresponds to the mass spectrum ion injection port.
In some embodiments, the height adjustment mechanism comprises:
the mounting plate is arranged below the carrying tray and is fixed with the carrying tray;
the screw is arranged below the mounting plate, and one end of the screw extends out of one side of the loading tray, which is far away from the mass spectrum ion injection port, to form an operation end;
the pushing piece is connected to the screw rod in a threaded mode and moves along the axial direction of the screw rod when the screw rod rotates;
the tilting part is rotatably arranged on the mounting plate, the upper end of the tilting part is positioned between the mounting plate and the carrying tray and abuts against one side of the carrying tray close to the operating end of the screw rod, the lower end of the tilting part corresponds to the pushing part so as to push the lower end of the tilting part when the pushing part moves along the screw rod, so that the tilting part rotates, and the upper end of the tilting part ascends or descends when the tilting part rotates; so as to push one side of the loading tray close to the operation end to lift or fall, so that the outlet of the capillary chromatographic column on the loading tray is lifted or lowered, and the mass spectrum ion injection port is aligned in the vertical direction.
In some embodiments, the apparatus further comprises a housing covering at least the mounting stage, the object stage and the object tray.
Compared with the prior art, the mobile electrospray ion source accurate positioning device with the linkage of the chromatographic valve has the beneficial effects that: the device uses an inductor to link with a chromatographic valve, converts the switching action (rotation) of the chromatographic valve into an electric signal so as to determine the capillary chromatographic column connected to the liquid chromatograph, and controls a driving mechanism to drive a carrying tray to move according to the capillary chromatographic column connected to the liquid chromatograph, so that the connected capillary chromatographic column is aligned to a mass spectrum ion injection port, and accurate positioning is realized; moreover, the full-automatic online switching and selection of the capillary chromatographic columns can be realized, the performance and application range of the instrument are expanded, and the automation level and the use efficiency of the instrument are improved.
Drawings
In the drawings, which are not necessarily drawn to scale, like reference numerals may describe similar components in different views. Like reference numerals having letter suffixes or different letter suffixes may represent different instances of similar components. The drawings illustrate various embodiments, by way of example and not by way of limitation, and together with the description and claims, serve to explain the inventive embodiments. The same reference numbers will be used throughout the drawings to refer to the same or like parts, where appropriate. Such embodiments are illustrative, and are not intended to be exhaustive or exclusive embodiments of the present apparatus or method.
Fig. 1 is a schematic perspective view of a mobile electrospray ion source precision positioning device linked with a chromatographic valve according to an embodiment of the invention.
Fig. 2 is a schematic perspective view of a mobile electrospray ion source precision positioning device with a removed carrier tray with a view angle, in which the chromatographic valve is linked according to an embodiment of the present invention.
Fig. 3 is a schematic perspective view of another embodiment of the present invention showing a precise positioning device for a chromatographic valve-linked mobile electrospray ion source with a carrier tray removed from its perspective view, wherein the top plate is removed from the transverse base, showing the internal structure thereof.
Fig. 4 is a schematic perspective view of another view of the mobile electrospray ion source precision positioning device with linkage of the chromatographic valve according to the embodiment of the present invention, wherein a capillary chromatographic column and a box body thereof are shown.
Fig. 5 is a schematic structural view of a carrier tray and a height adjusting mechanism in an embodiment of the present invention.
Fig. 6 is a partial structural schematic view of a carrier tray and a height adjustment mechanism in an embodiment of the present invention.
Fig. 7 is a control schematic diagram of a mobile electrospray ion source precision positioning device with linkage of a chromatographic valve according to an embodiment of the invention.
Reference numerals:
1-mounting a table; 2-avoiding space; 3-pin; 4-operating buttons; 5-a guide rail; 6-an objective table; 7-carrying pallet; 8-a transverse base; 9-mounting a plate; 10-a screw; 11-a pole base; 12-a knob; 13-pushing the piece; 14-a tilting member; 15-a pin shaft; 17-a magnetic proximity switch; 18-positioning a metal seek bar; 19-limit switch; 20-a controller; 21-a drive mechanism; 22-a support plate; 23-a first connection plate; 24-a second connecting plate; 25-capillary chromatography column; 26-capillary tubing; 27-a valve head; 28-interface; 29-box body.
Detailed Description
In order to make the objects, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the drawings of the embodiments of the present invention. It is to be understood that the embodiments described are only a few embodiments of the present invention, and not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the described embodiments of the invention without any inventive step, are within the scope of protection of the invention.
Unless defined otherwise, technical or scientific terms used herein shall have the ordinary meaning as understood by one of ordinary skill in the art to which this invention belongs. The use of "first," "second," and similar terms in the present application do not denote any order, quantity, or importance, but rather the terms are used to distinguish one element from another. The word "comprising" or "comprises", and the like, means that the element or item listed before the word covers the element or item listed after the word and its equivalents, but does not exclude other elements or items. The terms "connected" or "coupled" and the like are not restricted to physical or mechanical connections, but may include electrical connections, whether direct or indirect. "upper", "lower", "left", "right", and the like are used merely to indicate relative positional relationships, and when the absolute position of the object being described is changed, the relative positional relationships may also be changed accordingly.
To maintain the following description of the embodiments of the present invention clear and concise, a detailed description of known functions and known components of the invention have been omitted.
The embodiment of the invention provides a mobile electrospray ion source accurate positioning device linked with a chromatographic valve, which is used for connecting a liquid chromatograph and a mass spectrometer, wherein the liquid chromatograph is connected with the chromatographic valve, a valve head 27 of the chromatographic valve is provided with a plurality of interfaces 28 selectively communicated with the chromatographic valve, namely the valve head 27 is provided with a plurality of interfaces 28, and when the chromatographic valve rotates, different interfaces 28 can be communicated with the chromatographic valve. As shown in fig. 1, the mobile electrospray ion source precision positioning device (hereinafter referred to as "precision positioning device") with linkage of the chromatographic valve of the embodiment of the invention includes a connecting bracket, a carrying tray 7, a controller 20, an inductor and a driving mechanism 21, wherein the connecting bracket is detachably arranged on a mass spectrum panel (not shown in the figure) of the mass spectrometer; the carrying tray 7 is arranged on the connecting bracket; the sensor is connected with the controller 20 and used for sensing the rotation position information of the valve head 27 and sending the rotation position information to the controller 20; the controller 20 is connected with the driving mechanism 21, the controller 20 determines the interface 28 communicated with the liquid chromatograph according to the received rotation position information, because different interfaces 28 are connected with different capillary chromatographic columns 25 (the capillary chromatographic columns 25 are arranged on the carrying tray 7, and different capillary chromatographic columns 25 have specific positions on the carrying tray 7), the capillary chromatographic columns 25 connected into the liquid chromatograph can be determined according to the communicated interface 28, and the driving mechanism 21 is controlled to control the carrying tray 7 to move on the connecting bracket, so that the outlet of the capillary chromatographic columns 25 connected into the liquid chromatograph is opposite to the mass spectrum ion injection port on the mass spectrum panel.
The accurate positioning device provided by the embodiment of the invention can acquire switching information of a chromatographic valve of a liquid chromatograph, is linked with the switching of the chromatographic valve, can determine that the chromatographic valve is connected with an interface 28 of the liquid chromatograph, further determine a capillary chromatographic column 25 connected with the interface 28, and automatically control a carrying tray 7 carrying the capillary chromatographic column 25 to drive the capillary chromatographic column 25 to move together, so that the capillary chromatographic column 25 communicated with the liquid chromatograph is automatically switched to be opposite to a mass spectrum ion injection port of a mass spectrometer for mass spectrum detection; that is, the precise positioning device of the embodiment of the present invention can be linked with the switching of the chromatographic valve of the liquid chromatograph, thereby realizing the online automatic switching and selection of the capillary chromatographic column 25, improving the automation level and the use efficiency of the instrument, and expanding the performance and the application range of the instrument.
The following describes in detail a specific structure of an embodiment of the present invention with reference to the drawings.
With continuing to refer to fig. 1 to 4, the connecting bracket includes a mounting platform 1, a guide rail 5 and an object stage 6, the mounting platform 1 has an avoiding space 2 for avoiding an ion injection port of the mass spectrum, so that the ion injection port of the mass spectrum is directly opposite to an object carrying tray 7 through the avoiding space 2, the mounting platform 1 is detachably connected with a mass spectrum panel, in this embodiment, a through groove is formed in the middle of the mounting platform 1 to form the avoiding space 2, which is equivalent to the mounting platform 1 is attached to the mass spectrum panel and sleeved on the periphery of the injection port of the mass spectrum column; the guide rail 5 is fixed on the mounting table 1; the objective table 6 is movably arranged on the guide rail 5, the carrying tray 7 is arranged on the objective table 6, the guide rail 5 is arranged along the direction of the ion injection port of the mass spectrum, and is arranged into two parallel parts, so that the carrying tray 7 can stably move on the carrying tray, and when the capillary chromatographic column 25 is loaded and replaced, the capillary chromatographic column is moved away from the mass spectrum panel, the phenomenon that the outlet end of the capillary chromatographic column 25 is damaged due to the fact that the distance is too short is prevented, and after the loading and unloading are completed, the carrying tray 7 is pushed to a position suitable for the distance from the mass spectrum panel along the guide rail 5. The linking bridge can be used for installing whole accurate positioner detachably on the mass spectrum panel of mass spectrograph, is convenient for make capillary chromatographic column 25 be close to the mass spectrum ion injection mouth, carries out the mass spectrum and detects, and in non-user state, can detach accurate positioner from the mass spectrograph, prevents to hinder the use of mass spectrograph. The structure of the connecting bracket is not limited to the above structure, and those skilled in the art can select and determine the connecting bracket according to actual needs.
In some embodiments, with continued reference to fig. 1 and 2, the mounting table 1 is provided with a flat cylindrical pin 3 capable of rotating relative to the mounting table 1, the mass spectrometry panel is provided with a locking groove (not shown) for cooperating with the pin 3, the locking groove has a notch size smaller than a cavity size of the locking groove, so that the pin 3 can enter the locking groove when the pin 3 rotates to the position that the narrow side of the pin is opposite to the notch, and the pin 3 rotates to the position that the wide side of the pin is opposite to the notch and is limited in the locking groove, so that the mounting table 1 is fixed on the mass spectrometry panel. In addition, in order to rotate the pin 3 conveniently, a control button 4 is arranged at one end of the pin 3 protruding out of the mounting table 1, and the pin 3 is driven to rotate by pushing the control button 4. This embodiment has realized the detachable connection of mount table 1 and mass spectrum panel through the cooperation of pin 3 with locking groove, and connected mode easy operation, reliable and stable. The connection structure of the mounting table 1 and the mass spectrum panel can also adopt a mode such as spring buckle, screw thread and the like, and this embodiment is not specifically limited to this, as long as the mounting table 1 can be conveniently and stably installed on the mass spectrum panel.
In some embodiments, as shown in fig. 2 and fig. 3, the mobile positioning system further includes a transverse base 8, the transverse base 8 is fixed on the object stage 6, the object tray 7 is movably disposed on the transverse base 8, by providing the transverse base 8, the stroke of the object tray 7 in the transverse direction can be increased without increasing the transverse size of the object stage 6, so as to accommodate more capillary columns 25 in the transverse direction, the object tray 7 has enough moving space in the transverse direction, so that each capillary column 25 can be opposite to the mass spectrum ion injection port, and the applicability of the precision positioning device is further improved, so as to be used for switching more different capillary columns 25.
In the present invention, a mass spectrum panel is used as a reference object to determine a three-dimensional space, wherein the mass spectrum panel is vertically disposed, an upward side of the mass spectrum panel is an upper side, an upward side of the mass spectrum panel is a lower side, an opposite side of the mass spectrum panel is a front side, and a left-right direction of the mass spectrum panel is a transverse direction.
As shown in fig. 7, the sensor for sensing the rotation position information of the valve head 27 may be one or more types of sensors, for example, a photoelectric sensor, a distance sensor, etc. may be selected, and is not specifically limited herein, in consideration of the specific structure and installation space of the liquid chromatograph, the sensor of the present embodiment uses the magnetic proximity switch 17, specifically, the magnetic proximity switch 17 as the sensor includes a plurality of magnetic proximity switches 17 corresponding to the interfaces 28 of the valve head 27 one to one, as shown in fig. 7, the valve head 27 shown in the present embodiment has twelve interfaces 28, one magnetic proximity switch 17 is respectively arranged corresponding to each interface 28, that is, a total of twelve magnetic proximity switches 17 are arranged, and K is respectively arranged in fig. 71、K2、K3…K12(ii) a When a first interface (when the valve head 27 is rotated, the interface 28 which is conducted with the liquid chromatograph is defined as a first interface, that is, any one of the interfaces 28 can be a first interface) is conducted with the liquid chromatograph, a first magnetic proximity switch 17 corresponding to the first interface (when the interface 28 which is conducted with the liquid chromatograph is defined as a first magnetic proximity switch 17, that is, any one of the magnetic proximity switches 17 is a first magnetic proximity switch 17 when closed) is closed, and a corresponding position signal is generated, for example, after the first magnetic proximity switch 17 is closed, the electric potential on both sides of the first magnetic proximity switch is changed, for example, the high level is changed into the low level, so that the position signal is formed; the controller 20 determines that the first capillary chromatographic column 25 connected to the first interface (the capillary chromatographic column 25 connected to the first interface is defined as a first capillary chromatographic column) is connected to the liquid chromatograph according to the acquired position signal. Whenever the controller 20 determines the capillary chromatographic column 25 to be in communication with the liquid chromatographThe driving mechanism 21 can be controlled to drive the carrying tray 7 to drive the capillary chromatographic columns 25 to move together until the outlet of the first capillary chromatographic column 25 is opposite to the mass spectrum ion injection port, the controller 20 controls the driving mechanism 21 to stop driving, and at this time, mass spectrum detection can be carried out. In the embodiment, the switching action of the chromatographic valve is converted into an electric signal and sent to the controller 20, so that the switching and selection of the capillary chromatographic column 25 are automatically controlled on line, the operation is convenient, and the performance and the application of the liquid chromatography and mass spectrometry combined instrument are expanded.
In some embodiments, as shown in fig. 3 and 7, a plurality of positioning metal tracking bars 18 are arranged on the transverse base 8 at intervals along the horizontal direction perpendicular to the orientation direction of the ion injection port of the mass spectrum, and the positioning metal tracking bars 18 are connected with the magnetic proximity switches 17 one by one; the bottom of the loading tray 7 is provided with a contact (not shown in the figure) capable of being conducted with the positioning metal track seeking bar 18, the driving mechanism 21 includes a driving motor, such as a dc motor, which can be disposed in the transverse base 8, the driving motor drives the loading tray 7 to move on the transverse base 8 along the arrangement direction of the positioning metal track seeking bar 18 under the control of the controller 20, when the first magnetic proximity switch 17 is closed, the contact on the bottom of the loading tray 7 touches the first positioning metal track seeking bar 18 (the positioning metal track seeking bar 18 connected to the first magnetic proximity switch 17 is defined as the first positioning metal track seeking bar 18, and all the positioning metal track seeking bars 18 can be the first positioning metal track seeking bar 18) to generate the position signal. That is, when the first magnetic proximity switch 17 is closed, the first positioning metal trace seeking bar 18 connected to the first magnetic proximity switch 17 is powered on, when the driving motor drives the loading tray 7 to move so that the contact at the bottom of the loading tray contacts with the powered positioning metal trace seeking bar 18, a control signal is generated, the controller 20 controls the driving motor to stop driving the loading tray 7 according to the control signal, and at this time, the outlet of the first capillary chromatographic column 25 which is conducted with the liquid chromatograph corresponds to the mass spectrum ion injection port, so that accurate positioning of the loading tray 7 is realized.
In order to prevent the driving motor from moving excessively out of the layout area of the positioning metal track-seeking bars 18, in some embodiments, as shown in fig. 2 and 3, limit switches 19 are respectively disposed on two sides of the arrangement direction of the positioning metal track-seeking bars 18, so that the driving motor drives the loading tray 7 to move reversely in the case that the driving motor drives the loading tray 7 to move to trigger the limit switches 19. The limit switch 19 is electrically connected with the controller 20, when the drive motor touches the limit switch 19, a control signal is generated, and the controller 20 sends a control command to the drive motor after receiving the control signal, so as to control the drive motor to drive the loading tray 7 to move in the opposite direction of the current movement, so as to continue to search for the electrically connected positioning metal track seeking bar 18 until the electrically connected positioning metal track seeking bar 18 is searched.
In some embodiments, as shown in fig. 5 and 6, the fine positioning device further includes a height adjusting mechanism, which is disposed on the carrier tray 7 and is used for adjusting the position of the capillary chromatographic column 25 on the carrier tray 7 corresponding to the ion injection port of the mass spectrometer in the up-down direction. According to the accurate positioning device provided by the embodiment of the invention, the distance between the outlet end of the capillary chromatographic column 25 and the mass spectrum ion injection port in the front-back direction is adjusted by pushing the carrying tray 7 to move on the guide rail 5, the carrying tray 7 is driven to horizontally move on the transverse base 8 through the driving motor so as to adjust the distance between the outlet end of the capillary chromatographic column 25 and the mass spectrum ion injection port in the left-right direction, the carrying tray 7 is driven to move or rotate in the up-down direction through the height adjusting mechanism so as to adjust the distance between the outlet end of the capillary chromatographic column 25 and the mass spectrum ion injection port in the up-down direction, and finally the outlet end of the capillary chromatographic column 25 is aligned with the mass spectrum ion injection port, so that an ionized object to be detected is injected into the mass spectrum ion injection port and enters the mass spectrometer for detection.
As shown in fig. 5 and 6, the height adjustment mechanism provided in this embodiment includes a mounting plate 9, a screw 10, a pushing member 13, and a tilting member 14, where the mounting plate 9 is disposed below the loading tray 7 and fixed to the loading tray 7, one end of the screw 10 is rotatably connected to the bottom of the mounting plate 9, and the other end of the screw extends out of the loading tray 7 to form an operating end; in this embodiment, the carrier tray 7 includes a support plate 22 and a first connection plate 23 and a second connection plate 24 connected to opposite sides of the support plate 22, such that the carrier tray 7 forms a U-shaped structure with an opening facing downward, the first connection plate 23 faces and is close to the mass spectrometry particle injection port, the second connection plate 24 is far away from the mass spectrometry particle injection port, the screw is located below the support plate 22, and one end of the screw passes through and extends out of the second connection plate 24, and the other end of the screw is located between the first connection plate 23 and the second connection plate 24. The pushing piece 13 is connected to the screw 10 in a threaded manner, and when the screw 10 rotates, the pushing piece 13 does not rotate along with the screw, but moves along the axial direction of the screw 10; the tilting piece 14 is rotatably arranged on the mounting plate 9, and the upper end of the tilting piece is positioned between the mounting plate 9 and the supporting plate 22 of the carrying tray 7, and abuts against one side of the bearing plate 22 close to the operating end of the screw 10 (the operating end of the screw 10 is the end far away from the spectrum ion injection port, and the axial direction of the screw 10 is perpendicular to the arrangement direction of the positioning metal track seeking bars 18), the lower end of the tilting member 14 corresponds to the pushing member 13, so as to push the lower end of the tilting member 14 to rotate when the pushing member 13 moves along the screw rod 10, and the upper end of the tilting member 14 ascends or descends when the tilting member 14 rotates, when the upper end of the tilting member 14 ascends or descends, the supporting plate 22 is pushed to lift or fall on the side close to the operation end, so that the outlet end of the capillary chromatographic column 25 on the supporting plate ascends or descends, and the outlet end of the capillary chromatographic column 25 can move in the up-down direction to be aligned with the mass spectrum ion injection port. That is, the two ends of the carrying tray 7 are lifted up and down like a seesaw to drive the two ends of the capillary chromatographic column 25 thereon to ascend or descend, so as to align the capillary chromatographic column 25 with the mass spectrum ion injection port. It should be noted that the height adjusting mechanism is not limited to the above structure, and a person skilled in the art can select the height adjusting mechanism according to actual needs, and this embodiment is not limited in this respect.
With continued reference to fig. 5 and 6, in some embodiments, a rod seat 11 is fixed at the bottom of the mounting plate 9, one end of the screw rod 10 is rotatably connected in the rod seat 11, and a knob 12 for rotating the screw rod 10 is installed at the operation end of the screw rod 10; the second connecting plate 24 is provided with an approximately semicircular through groove which is convenient for the screw 10 to extend out of the loading tray 7 and avoid the screw 10. The tilting piece 14 is a lever similar to an L shape and comprises a short arm and a long arm, the pushing piece 13 is plate-shaped, the plate surface of the pushing piece acts on the outer side of the short arm of the L-shaped piece, the outer vertex angle of the free end of the long arm acts on the carrying tray 7, and the joint of the long arm and the short arm becomes a pivot point and is rotatably connected to the mounting plate 9 through a pin shaft 15.
The accurate positioning device of the embodiment of the invention enables the outlet position of the capillary chromatographic column 25 to be adjustable relative to the position of the mass spectrum ion injection port, so that the signal intensity of ions to be detected in the mass spectrum is suitable for relevant detection requirements, the requirements that different samples to be detected need different distances are met, and the adjustment achieves the effects that the ions need to be over against the mass spectrum ion injection port in the vertical direction and the left-right direction, and the distance in the front-back direction is within the range of 1mm-10 mm.
In some embodiments, the precise positioning device further includes a cover (not shown), the shape and material of the cover are not limited, and the cover is at least covered on the mounting table 1, the object stage 6 and the object tray 7 to form a closed or semi-closed environment for the precise positioning device, so as to protect the precise positioning device and prolong the service life of the precise positioning device.
It should be noted that the manner of installing the capillary column 25 on the carrier tray 7 is not limited, and those skilled in the art may select the installation according to the needs, and as shown in fig. 4, the capillary column 25 of the present embodiment is installed on the carrier tray 7 through the box 29, and the box 29 can move together with the carrier tray 7. The capillary chromatographic column 25 is connected with the interface 28 of the chromatographic valve through the capillary tube 26, and the liquid-phase substance to be detected in the capillary tube 26 is ionized and charged under the action of high voltage in the box body 29 before entering the capillary chromatographic column 25, so that the liquid-phase substance to be detected is subjected to electrospray ionization (becoming gas-phase charged ions) at the injection outlet of the capillary chromatographic column 25 and then enters the mass spectrum injection port, and the detection is completed.
According to the accurate positioning device provided by the embodiment of the invention, the sensor is used for converting the switching action of the chromatographic valve into an electric signal, and the driving motor is used for driving the carrying tray 7 to realize accurate positioning through the positioning metal tracing strip 18, so that the electronic control capillary chromatographic column 25 synchronous with the switching of the chromatographic valve is moved and positioned, the full-automatic online switching and selection of the capillary chromatographic column 25 are realized, the performance and application range of an instrument are expanded, and the automation level and the use efficiency of the instrument are improved.
The above embodiments are only exemplary embodiments of the present invention, and are not intended to limit the present invention, and the scope of the present invention is defined by the claims. Various modifications and equivalents may be made by those skilled in the art within the spirit and scope of the present invention, and such modifications and equivalents should also be considered as falling within the scope of the present invention.
Claims (10)
1. The utility model provides a accurate positioner of portable electrospray ion source of chromatogram valve linkage for the connection of liquid chromatograph and mass spectrograph, the liquid chromatograph is connected with the chromatogram valve, the valve head of chromatogram valve have selectively with a plurality of interfaces of chromatogram valve intercommunication, characterized in that, the accurate positioner of portable electrospray ion source of chromatogram valve linkage includes:
the connecting bracket is detachably arranged on a mass spectrum panel of the mass spectrometer;
the carrying tray is arranged on the connecting bracket;
a controller;
the sensor is connected with the controller and used for sensing the rotating position information of the valve head and sending the rotating position information to the controller;
a drive mechanism connected to the controller; wherein the content of the first and second substances,
the controller determines an interface of the valve head communicated with the liquid chromatograph according to the received rotation position information so as to determine a capillary chromatographic column connected to the liquid chromatograph, and controls the driving mechanism to enable the carrying tray to drive the capillary chromatographic column to move on the connecting support, so that an outlet of the capillary chromatographic column is opposite to a mass spectrum ion injection port on the mass spectrum panel.
2. The valve-linked mobile electrospray ion source precise positioning device according to claim 1, wherein the sensor comprises a plurality of magnetic proximity switches corresponding to the interfaces of the valve head in a one-to-one manner, when a first interface is conducted with the liquid chromatograph, a first magnetic proximity switch corresponding to the first interface is closed to generate a corresponding position signal;
the controller determines a first capillary chromatographic column communicated with the liquid chromatograph according to the acquired position signal generated by the first magnetic proximity switch, and controls the driving mechanism to drive the carrying tray to move, so that an outlet of the first capillary chromatographic column is opposite to the mass spectrum ion injection port on the mass spectrum panel.
3. The apparatus of claim 2, further comprising the transverse base, wherein the transverse base is fixed to the stage, and the carrier tray is movably disposed on the transverse base.
4. The valve-linked mobile electrospray ion source precise positioning device according to claim 3, wherein a plurality of positioning metal tracing strips are arranged on the transverse base at intervals along a horizontal direction perpendicular to the orientation direction of the mass spectrum ion injection port, and the positioning metal tracing strips are connected with the magnetic proximity switches one by one;
the bottom of the carrying tray is provided with a contact which can be conducted with the positioning metal tracing strip;
the driving mechanism comprises a driving motor, the driving motor is arranged in the transverse base and used for driving the carrying tray to move on the transverse base along the arrangement direction of the positioning metal tracing strips, when the first magnetic approach switch is closed, the contact on the bottom of the carrying tray is touched by the first positioning metal tracing strips to generate the position signal, and meanwhile, the driving motor stops rotating.
5. The apparatus of claim 4, wherein limit switches are respectively disposed on two sides of the alignment direction of the positioning metal track bars, so that when the driving motor drives the carrying tray to move to trigger the limit switches, the driving motor drives the carrying tray to move in a reverse direction.
6. The valve-linked mobile electrospray ion source precision positioning device of claim 1, wherein the connecting bracket comprises:
the mounting table is provided with an avoiding space for avoiding the mass spectrum ion injection port so that the mass spectrum ion injection port is directly opposite to the carrying tray through the avoiding space, and the mounting table is detachably connected with the mass spectrum panel;
a guide rail fixed to the mount table;
the object stage is movably arranged on the guide rail, and the object tray is arranged on the object stage.
7. The apparatus of claim 6, wherein the mounting table is provided with a flat cylindrical pin capable of rotating relative to the mounting table, the mass spectrum panel is provided with a locking groove for matching with the pin, and the locking groove has a notch size smaller than that of the locking groove, so that the pin can enter the locking groove when rotating to the narrow side opposite to the notch, and the pin is limited in the locking groove when rotating to the wide side opposite to the notch, so that the mounting table is fixed on the mass spectrum panel.
8. The apparatus of claim 1, further comprising a height adjustment mechanism disposed on the carrier tray for adjusting the height of the carrier tray so that the capillary chromatography column on the carrier tray is located at a position corresponding to the mass spectrometry ion injection port in the vertical direction.
9. The valve-linked mobile electrospray ion source precision positioning device of claim 8, wherein the height adjustment mechanism comprises:
the mounting plate is arranged below the carrying tray and is fixed with the carrying tray;
the screw is arranged below the mounting plate, and one end of the screw extends out of one side of the loading tray, which is far away from the mass spectrum ion injection port, to form an operation end;
the pushing piece is connected to the screw rod in a threaded mode and moves along the axial direction of the screw rod when the screw rod rotates;
the tilting part is rotatably arranged on the mounting plate, the upper end of the tilting part is positioned between the mounting plate and the carrying tray and abuts against one side of the carrying tray close to the operating end of the screw rod, the lower end of the tilting part corresponds to the pushing part so as to push the lower end of the tilting part when the pushing part moves along the screw rod, so that the tilting part rotates, and the upper end of the tilting part ascends or descends when the tilting part rotates; so as to push one side of the loading tray close to the operation end to lift or fall, so that the outlet of the capillary chromatographic column on the loading tray is lifted or lowered, and the mass spectrum ion injection port is aligned in the vertical direction.
10. The apparatus of claim 2, further comprising a housing covering at least the mounting table, the stage and the carrier tray.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010274334.3A CN113514592A (en) | 2020-04-09 | 2020-04-09 | Movable electrospray ion source accurate positioning device linked with chromatographic valve |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010274334.3A CN113514592A (en) | 2020-04-09 | 2020-04-09 | Movable electrospray ion source accurate positioning device linked with chromatographic valve |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN113514592A true CN113514592A (en) | 2021-10-19 |
Family
ID=78060625
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202010274334.3A Pending CN113514592A (en) | 2020-04-09 | 2020-04-09 | Movable electrospray ion source accurate positioning device linked with chromatographic valve |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN113514592A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114371211A (en) * | 2021-12-31 | 2022-04-19 | 四川红华实业有限公司 | Accurate fine setting positioner of mass spectrometer electro-magnet |
-
2020
- 2020-04-09 CN CN202010274334.3A patent/CN113514592A/en active Pending
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114371211A (en) * | 2021-12-31 | 2022-04-19 | 四川红华实业有限公司 | Accurate fine setting positioner of mass spectrometer electro-magnet |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| AU604021B2 (en) | Control mechanism for computer keyboard and the like | |
| CN207036761U (en) | A kind of full-automatic point sample instrument | |
| CN113514592A (en) | Movable electrospray ion source accurate positioning device linked with chromatographic valve | |
| CN105845506B (en) | A kind of relay overtravel parameter setting apparatus | |
| CN212060105U (en) | Movable electrospray ion source accurate positioning device linked with chromatographic valve | |
| CN109551433B (en) | Carrier capable of being replaced quickly and identified automatically | |
| EP2304766A2 (en) | Control of the positional relationship between a sample collection instrument and a surface to be analyzed during a sampling procedure using a laser sensor | |
| CN113514593A (en) | Multi-channel capillary chromatographic column electrospray ion source device | |
| CN102980787B (en) | Locator is inhaled in the sampling detected for water-based | |
| CN212159715U (en) | Multi-channel capillary chromatographic column electrospray ion source device | |
| CN217009119U (en) | Electrospray ion source capable of automatically adjusting spray position and mass spectrometer | |
| Thompson et al. | Investigation of electrospray ionization and electrostatic focusing devices using a three-dimensional electrospray current density profiler | |
| CN113776660A (en) | Transformer vibration detection equipment | |
| CN109333421A (en) | A kind of automobile door plate snap-fitted tooling | |
| CN212572666U (en) | Testing device of electronic product | |
| CN220752017U (en) | Three-dimensional biological mass spectrum imaging sampling device of probe electrospray ion source | |
| US5340010A (en) | Bonding apparatus | |
| Wang et al. | Microliter-level multi-channel fraction collector for high-throughput separation system | |
| CN112017940A (en) | Mass spectrometer sample target inlet and outlet device and mass spectrometer | |
| CN115440572B (en) | Mass spectrometry device and method | |
| CN110641299A (en) | A high-efficient type AGV dolly charging device for intelligent commodity circulation | |
| CN212320664U (en) | Steel bar protective layer thickness test equipment for building engineering | |
| JPS6150727A (en) | Xy table device and operating machine with said device | |
| CN217332279U (en) | Mass spectrum motion platform | |
| JP2002243404A (en) | Magnetic proximity switch |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination |