CN113508813B - Rooting agent for alum root cutting and alum root cutting propagation method - Google Patents

Rooting agent for alum root cutting and alum root cutting propagation method Download PDF

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CN113508813B
CN113508813B CN202110326059.XA CN202110326059A CN113508813B CN 113508813 B CN113508813 B CN 113508813B CN 202110326059 A CN202110326059 A CN 202110326059A CN 113508813 B CN113508813 B CN 113508813B
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孙翊
杨贞
张永春
李心
王桢
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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
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    • A01N43/34—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom
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    • A01G2/00—Vegetative propagation
    • A01G2/10—Vegetative propagation by means of cuttings
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
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Abstract

The invention discloses a rooting agent for alum root cutting and a alum root cutting propagation method. The active ingredient of the rooting agent for the alum root cutting is indolebutyric acid with the use concentration of 500-1000 mg/L. The rooting agent is suitable for spring cutting of alum roots, and can improve the rooting rate, plant height, root length and leaf number of 3 alum root varieties. The rooting agent is also suitable for autumn cuttage of alum roots, and can obviously improve the rooting rate, the plant height and the leaf number of the red alert variety which is difficult to root.

Description

Rooting agent for alum root cutting and alum root cutting propagation method
Technical Field
The invention belongs to the field of plant cultivation, and particularly relates to a rooting agent for alum root cutting and a alum root cutting propagation method.
Background
The alum root (Heuchera micrantha) is perennial herb flower of alum in Saxifragaceae, and is originally produced in the middle part of America. The alum root is resistant to yin, cool and waterlogging, has rich leaf color and is one of the most important ornamental characters. The alum root can be enjoyed in four seasons in warm areas in winter, and has an important position in garden application.
At present, the domestic research on the alum root mainly comprises growth adaptability research, adversity stress research, tissue culture technology research, garden application research and the like, and the research on the optimization of the alum root vegetative propagation technology is less. There are four main ways of alum root propagation, which are cutting, plant division, sowing and tissue culture. The plant division propagation is limited by the number of parent plants, the propagation coefficient is low, and the period is long; the original characters are easy to change because the sowing propagation is not asexual propagation; although the tissue culture can be carried out in a bottle for rapid propagation, the period is longer because seedling hardening, seedling emergence and the like are needed. The asexual propagation method can quickly obtain plants with consistent large-scale characters by cuttage, and is one of the main ways for producing high-quality alum root seedlings in industry. However, the alum root cutting propagation has the problem of low survival rate at present, and is not beneficial to the control of production cost. And when the alum root cutting seedlings are processed by selecting a proper plant growth regulator and concentration during cutting, the rooting rate and the survival rate of the alum root cutting seedlings can be greatly improved, the cost can be saved, the cutting efficiency is improved, and the method has important significance in industry.
CN 107135927A discloses a alum root cutting rapid propagation method, disclosing NAA and IAA solution with 250-1000ppm of rooting agent, and NAA: the mass ratio of IAA is 2-4:1. The rooting agent adopted in the patent can achieve a good rooting effect on part of varieties, and the seedling rate can reach more than 95%. However, because of the difference of varieties, the rooting effect of cuttage is not ideal under the condition that the rooting agent disclosed in the patent is used for the difficultly rooted varieties. For example, the rooting rate of the alum root with red warning is only about 45%, which obviously restricts the production efficiency.
Disclosure of Invention
In order to solve the technical problems, the invention aims to provide a more efficient alum root rooting agent and an alum root cutting propagation method.
Firstly, the invention provides a rooting agent for alum root cutting, wherein the effective component of the rooting agent is indolebutyric acid with the use concentration of 500-1000 mg/L.
Wherein, the rooting agent for the alum root cutting is a liquid preparation or a preparation which can be prepared into liquid when in use.
The invention also provides a alum root cutting propagation method, which adopts a quick dipping method to treat alum root cuttings with the rooting agent, and the treated alum root cuttings are cut in a matrix.
Wherein the cutting medium is turf: coconut chaff: the volume ratio of perlite is 7.
In one embodiment of the invention, the cuttage is carried out in a greenhouse, and the temperature in the daytime is controlled to be 20-25 ℃ and the temperature at night is controlled to be 15-18 ℃; spraying and moisturizing for 1 time every 3-4 hours in the daytime at the initial stage of cuttage; while keeping the greenhouse well ventilated.
The invention takes 3 alum root varieties of 'red warning', 'rio' and 'lymrhiji' as test materials, and adopts indolebutyric acid (IBA), naphthylacetic acid (NAA), rooting powder (ABT) and paclobutrazol (PP) with the concentrations of 500, 1000 and 2000mg/L in spring 333 ) The fast dipping method is used for treating alum root cutting seedlings, and 13 seedlings are treated for each variety. Measuring and counting the rooting rate, plant height, root length and leaf number of the cutting seedlings, carrying out difference significance analysis by SPSS software, and researching 3 alum root varieties subjected to different treatmentsCutting rooting and growth influence. The method is characterized in that a red alert alum root variety is used as a test material to carry out autumn cuttage, and alum root cutting seedlings are treated by a fast dipping method by adopting indolebutyric acid (IBA) with the concentration of 250, 500, 750 and 1000mg/L, 250ppm of rooting agent (NAA: IBA = 4:1), 500ppm of rooting agent (NAA: IBA = 3:1) and 1000ppm of rooting agent (NAA: IBA = 2:1). The rooting rate, the plant height and the leaf number of the cutting seedlings are measured and counted, and the SPSS software is used for carrying out difference significance analysis to research the influence of different treatments on the 'red alert' autumn cutting rooting and the growth of the alum root variety. Results show that 500mg/L and 1000mg/L of IBA are suitable for spring cutting of the alum root, and can improve the rooting rate, plant height, root length and leaf number of 3 alum root varieties. The 1000mg/L IBA is suitable for autumn cuttage of the alum root variety 'red alert', and can improve the rooting rate, plant height and leaf number of 'red alert'.
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Fig. 1 shows the rooting rates of alum 'red warning', 'rio' and 'lymrhiki' under different treatments.
FIG. 2 shows the plant heights of alum 'red warning', 'rio' and 'lymeryl' under different treatments.
Fig. 3 shows the root length of alum 'red warning', 'rio', 'lymeryl' under different treatments.
Fig. 4 shows the number of alum 'red guard', 'rio' and 'lymrik' leaves for different treatments.
FIG. 5 shows the rooting rate of the "red alert" of alum roots under different treatments.
FIG. 6 shows the plant height of alum root 'red guard' under different treatments.
Figure 7 shows the number of "red alert" leaves of alum roots under different treatments.
Detailed Description
The following examples are intended to illustrate the invention but are not intended to limit the scope of the invention.
Example 1 spring cutting test
The test materials are three alum varieties of 'red warning', 'rio' and 'lymriky'. And selecting healthy cutting slips as cutting materials. Selecting imported grass carbon: coconut husk: the volume ratio of perlite is 7.
Spring cutting tests were carried out in a greenhouse in Shanghai Yimiao GmbH base at 2.4.2020. Adopting IBA, NAA, ABT and PP 333 Each of the four plant growth regulators was set at 3 concentrations of 500, 1000 and 2000mg/L. Wherein IBA, NAA, and PP 333 For the production of the industrial bioengineering (Shanghai) GmbH, ABT (Ai Bidi) rooting powder (30% IAA;20% NAA) is produced by the research and development center of Beijing Ai Bidi. And (3) setting 13 treatments for each variety, wherein one treatment is blank control, each treatment is 24 cutting seedlings, the cutting seedlings are treated by adopting a method of dipping for 5s, and 3 times of repetition are set. After the cuttage is finished, the rooting condition of the cuttage seedlings is observed every day, and the rooting rate, the plant height, the root length and the leaf number are counted after 35 days.
TABLE 1 spring cuttage test design
Figure BDA0002994718950000041
Data measurements and statistics were performed on the test material cut in spring on day 5, month 8 of 2020. For each treatment, the rooting rate of 24 plants (rooting rate (%) = number of roots/total number) was counted, and 3 groups were counted. For each treatment, 5 plants were sampled at random and the plant height, root length and leaf number were counted. The SPSS software performs a differential significance analysis between different treatments (P < 0.05).
Influence of different treatments on rooting rate of alum root cutting
The rooting rate of the alum root cuttage is affected differently by different plant growth regulator treatments. Differences also exist between different varieties, as shown in FIG. 1.
The SPSS analysis result shows that under IBA (500 and 1000 mg/L), the 'red alert' rooting rate of the alum root variety is remarkably improved, and is respectively improved by 30.43% and 26.09% compared with CK, and no remarkable difference exists among 2 treatments. When the IBA concentration is increased to 2000mg/L, the rooting rate is reduced compared with IBA (500 and 1000 mg/L), but no obvious effect is produced with CKThe difference in the degree of tackiness. In PP 333 Under the treatment of (500 and 1000 mg/L), the 'red alert' rooting rate of the alum root variety is remarkably improved compared with CK, the rooting rate is improved by 30.43% and 15.21%, and no remarkable difference exists among 2 treatments. When PP is used 333 When the concentration is increased to 2000mg/L, the rooting rate is compared with that of PP 333 (500, 1000 mg/L) is reduced, but has no significant difference with CK. Under the treatment of NAA and ABT, the 'red alert' rooting rate of the alum root variety is reduced, and the high-concentration NAA (2000 mg/L) and ABT (2000 mg/L) perform better than the low-concentration NAA (1000 mg/L) and ABT (500 and 1000 mg/L). According to the data result display, IBA and PP 333 The lower the concentration of (2), the higher the rooting rate, and the lower the concentrations of IBA (500 mg/L) and PP 333 (500 mg/L) is beneficial to the rooting of the alum root variety in 'red alert' spring.
The alum species 'rioo' are in IBA (500, 1000 mg/L), NAA (2000 mg/L), ABT (500, 1000, 2000 mg/L) and PP 333 The rooting rate is higher under the treatments of (500, 1000 and 2000 mg/L), no significant difference exists among the treatments, and no significant difference exists between CK, wherein in PP 333 The rooting rate is highest under the treatment of (500 mg/L), and is 97.22%. The rooting rate under the treatment of high-concentration NAA (2000 mg/L) and ABT (2000 mg/L) is 95.83 percent and 95.83 percent, and the rooting rate is better than that of low-concentration NAA (500, 1000 mg/L) and ABT (500, 1000 mg/L), thereby being more beneficial to the rooting of the alum variety 'rioo'. IBA, PP 333 The lower the concentration of (A), the higher the rooting rate, and the lower the concentration of IBA and PP 333 Is favorable for the rooting of the alum root variety Rio' in IBA (500 mg/L) and PP 333 Under the treatment of (500 mg/L), the rooting rate respectively reaches 95.83 percent and 97.22 percent.
The rooting rate of the alum root variety 'Lymliki' is higher, and the rooting rate of 11 types under 13 types of treatments reaches 100 percent. Under NAA (1000, 2000 mg/L) treatment, the rooting rate is 98.61%, and the treatment has no significant difference with other 11 treatments.
Influence of different treatments on the plant height of alum root cutting
The results of SPSS analysis showed that the samples were analyzed in IBA (1000, 2000 mg/L), NAA (500, 1000, 2000 mg/L), ABT (1000 mg/L), PP 333 Under the treatment of (500, 1000 and 2000 mg/L), the red guard plant height of the alum root variety is obviously improved compared with CKThere were no significant differences between the 9 treatments. Under IBA (500, 1000 mg/L), the plant height is increased by 11.20% and 13.3% compared with CK.
The alum root varieties 'rioo' are IBA (500, 1000 mg/L), NAA (1000, 2000 mg/L), ABT (500, 1000, 2000 mg/L), PP 333 The plant height is remarkably improved compared with CK under the treatment of (500 mg/L), and no remarkable difference exists among 8 treatments. IBA (2000 mg/L), NAA (500 mg/L), PP 333 The plant heights of the alum root variety 'rio' treated by the 4 kinds of treatment (1000 and 2000 mg/L) have no significant difference with CK (figure 2).
The plant height of the alum root variety 'Lyme base' is remarkably improved compared with CK under the treatment of IBA (500, 1000, 2000 mg/L), NAA (2000 mg/L) and ABT (500, 1000 mg/L). Wherein, under the treatment of IBA (500, 1000 mg/L), the plant height of the alum root variety 'Lymliki' is the highest and respectively reaches 14.18cm and 14.02cm, which are respectively increased by 29.38 percent and 27.92 percent compared with CK.
Influence of different treatments on the root length and leaf number of alum root cutting
The SPSS analysis results showed that at low concentrations of IBA (500 mg/L), the 'red guard' root length and leaf number increased from CK, but there was no significant difference. High concentrations of ABT and PP 333 Under the treatment, the root length and the leaf number of the alum root variety 'red alert' are obviously reduced compared with CK. The test results show that PP 333 High concentration of NAA and high concentration of ABT can inhibit growth of alum leaf, high concentration of ABT and high concentration of PP 333 Meanwhile, the growth of the root length of alum can be inhibited, the high-concentration IBA has little influence on the root length and the growth of leaves, and the low-concentration IBA can promote the growth of the root length and the leaf number (figures 3-4).
High concentration of NAA, high concentration of ABT and PP 333 The treatment inhibited the increase in the number of alum 'rio' leaves, which was significantly reduced compared to CK. High concentration of PP 333 The treatment inhibits the growth of the alum 'rio' root. Under the treatment of IAA and NAA, the root length of the alum root variety 'rio' is remarkably improved compared with CK.
PP 333 The treatment inhibits the growth of the root length of the alum root variety 'lyme'. Low concentration of IThe BA (500, 1000 mg/L) treatment promoted the growth of root length, and was significantly improved compared to CK. Low concentrations of IBA (500, 1000 mg/L) treatment also promoted leaf number increase, but with no significant difference from CK.
The experimental results show that IBA (500 mg/L) treatment can promote the growth of root length and the increase of leaf number of alum root varieties 'red alert', 'rio' and 'lymrhiki'.
Example 2 autumn cutting test
In autumn, a alum root variety with low rooting rate is selected and tested with 'red warning', the test is carried out in 26 days 10 and 10 months in 2020 year, 250, 500, 750 and 1000mg/L indolebutyric acid (IBA) and 250ppm rooting agent (NAA: IBA = 4:1), 500ppm rooting agent (NAA: IBA = 3:1) and 1000ppm rooting agent (NAA: IBA = 2:1) in the alum root cutting and rapid propagation method (invention patent application number: 201710440334.4) are used. And 8 treatments in total, wherein one treatment is blank Control (CK), each treatment is carried out on 24 cutting seedlings, the cutting seedlings are treated by adopting a method of fast dipping for 5s, and 3 times of repetition are set. After the cuttage is finished, the rooting condition of the cuttage seedlings is observed every day, and the rooting rate, the plant height and the leaf number are counted after 35 days.
TABLE 2 autumn cuttage test design
Figure BDA0002994718950000071
And respectively carrying out data measurement and statistics on the test materials subjected to autumn cutting in 12-29 days in 2020. For each treatment, the rooting rate of 24 plants (rooting rate (%) = number of roots/total number) was counted, and three groups were counted. For each treatment, 5 plants were sampled at random and the plant height, root length and leaf number were counted. The SPSS software performs a differential significance analysis between different treatments (P < 0.05).
Influence of different treatments on rooting rate of alum root cutting
Under the condition of treating the alum root variety 'red warning' by 5-4 (IBA of 1000 mg/L), the rooting rate is obviously improved compared with the other 7 treatments, wherein the rooting rate is improved by 30.19 percent compared with CK. The rooting rate of 5-1, 5-2 and 5-3 is obviously improved compared with that of CK, and is respectively improved by 7.55%, 7.55% and 9.43%, and no obvious difference exists among 3 treatments. The rooting rate is lower under the treatment of 6-1, 6-2 and 6-3, and is only 44.44-45.83 percent. The 1000mg/L IBA is more beneficial to the rooting of the alum root variety in red alert autumn (figure 5), and the rooting rate can reach 69.44%.
Influence of different treatments on the plant height of alum root cutting
Under the treatment of 5-1, 5-2, 5-3, 5-4 and 6-1, the plant height of the alum root variety is remarkably improved compared with CK, the plant height is respectively improved by 19.18%, 22.24%, 18.37%, 15.10% and 18.37%, no remarkable difference exists among 5 treatments, and no remarkable difference exists between 6-2 and 6-3 treatments and CK. IBA treatment and low concentration ABT treatment (250 mg/L, NAA: IBA = 4:1) are more beneficial to increase the 'red alert' plant height of the alum root variety (FIG. 6).
Influence of different treatments on the root length and leaf number of cutting of alum root
The alum root varieties with red alert have no significant difference from CK under the treatment of 5-1, 5-2, 5-3, 5-4 and 6-2, and the number of leaves is significantly reduced under the treatment of 6-1 and 6-3 (figure 7).
The results of this study showed IBA (500, 1000 mg/L) and PP 333 (500 mg/L and 1000 mg/L) can improve the rooting rate of the alum root cutting slips, the NAA and ABT effects are poor, and the effect of the four plant growth regulators is IBA>PP 333 >NAA>ABT. The 'red alert' rooting rate of the alum root varieties treated by 1-1, 1-2, 4-1 and 4-2 is obviously improved compared with CK. Under the treatment of 1-2, 4-1 and 4-2, the red warning plant height of the alum root variety is obviously improved compared with CK. However, under the treatment of 4-1 and 4-2, the 'red alert' root length and leaf number ratio CK of the alum root variety are reduced.
Comprehensive comparison shows that the treatment most suitable for the 'red alert' spring cutting growth of the alum root variety is 1-2 and 1-1. Under the treatment of 1-1, 1-2, 2-3, 3-1, 3-2, 3-3 and 4-1, the rooting rate of the alum root variety 'rio' is higher, and no significant difference is generated between the alum root variety 'rio' and CK. The plant height of the strain has no significant difference among the 7 treatments, and each treatment is significantly improved compared with CK. Wherein, under the treatment of 1-1 and 1-2, the growth of the alum root variety 'rio' is obviously improved compared with CK. The most suitable for the cuttage growth of the alum root variety 'Liao' in spring is 1-1 and 1-2. The rooting rate of the alum root variety 'Lymliki' is higher, and the rooting rate of 11 types under 13 types of treatments reaches 100 percent. Under the treatment of 1-1 and 1-2, the plant height and the root length of the alum root variety 'lymeryl' are obviously improved compared with CK. The most suitable for the cuttage growth of the alum root variety 'Lyme base' in spring is 1-1 and 1-2. In conclusion, IBA at 500, 1000mg/L is most suitable for plant growth regulator treatment of alum root varieties 'Red alert', 'Riao' and 'Lyme base' spring cuttings.
In autumn, plant rooting requires higher concentrations of plant growth regulator stimulation than in spring. In the autumn cutting test, the IBA treatment is obviously superior to the rooting agent treatment in the prior patent, and 1000mg/L IBA is more suitable for the cutting growth of the alum root variety 'red alert' in autumn. The conclusion provides scientific basis for the high-efficiency cutting propagation of the alum root in the industry.
The foregoing is only a preferred embodiment of the present invention, and it should be noted that, for those skilled in the art, various modifications and decorations can be made without departing from the technical principle of the present invention, and these modifications and decorations should also be regarded as the protection scope of the present invention.

Claims (1)

1. The 'red alert' autumn cutting propagation method of the alum root variety is characterized in that the alum root cutting slips are treated by 1000mg/L indolebutyric acid by a quick dipping method and are cut in a substrate after treatment, wherein the cutting substrate is turf: coconut husk: the volume ratio of perlite is 7; spraying and moisturizing for 1 time every other 3 to 4 hours in the daytime at the early stage of cuttage; while keeping the greenhouse well ventilated.
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Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109757369A (en) * 2017-11-09 2019-05-17 江苏省中国科学院植物研究所 A kind of method of alum root kind " obsidian " tissue culture propagating
CN109757368A (en) * 2017-11-09 2019-05-17 江苏省中国科学院植物研究所 The method that alum root kind " kilim carpet " rachis vitro Regeneration System is established

Family Cites Families (3)

* Cited by examiner, † Cited by third party
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CN107135927B (en) * 2017-06-12 2020-08-07 云南省农业科学院花卉研究所 Cuttage and rapid propagation method for alum roots
CN109757371A (en) * 2017-11-10 2019-05-17 江苏省中国科学院植物研究所 A kind of method of alum root kind " tranquil oasis " tissue culture propagating
CN109122315B (en) * 2018-08-24 2021-06-29 上海市农业科学院 Method for cultivating seedlings by using alum root and petioles

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109757369A (en) * 2017-11-09 2019-05-17 江苏省中国科学院植物研究所 A kind of method of alum root kind " obsidian " tissue culture propagating
CN109757368A (en) * 2017-11-09 2019-05-17 江苏省中国科学院植物研究所 The method that alum root kind " kilim carpet " rachis vitro Regeneration System is established

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