CN113508180A - 用于染色质作图测定的dna条形码化核小体 - Google Patents

用于染色质作图测定的dna条形码化核小体 Download PDF

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Publication number
CN113508180A
CN113508180A CN201980092668.4A CN201980092668A CN113508180A CN 113508180 A CN113508180 A CN 113508180A CN 201980092668 A CN201980092668 A CN 201980092668A CN 113508180 A CN113508180 A CN 113508180A
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Prior art keywords
nucleosome
dna
nucleosomes
polynucleosome
transposase
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CN201980092668.4A
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Chinese (zh)
Inventor
M·W·考尔斯
Z-W·孙
M-C·基奥
R·J·文特雷斯
E·N·温扎普菲
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Love Pizza Co
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Love Pizza Co
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6875—Nucleoproteins
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2500/00—Screening for compounds of potential therapeutic value
    • G01N2500/10—Screening for compounds of potential therapeutic value involving cells

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Urology & Nephrology (AREA)
  • Biomedical Technology (AREA)
  • Hematology (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Peptides Or Proteins (AREA)
CN201980092668.4A 2018-12-21 2019-12-20 用于染色质作图测定的dna条形码化核小体 Pending CN113508180A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201862783861P 2018-12-21 2018-12-21
US62/783,861 2018-12-21
PCT/US2019/067735 WO2020132388A1 (en) 2018-12-21 2019-12-20 Dna-barcoded nucleosomes for chromatin mapping assays

Publications (1)

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CN113508180A true CN113508180A (zh) 2021-10-15

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CN201980092668.4A Pending CN113508180A (zh) 2018-12-21 2019-12-20 用于染色质作图测定的dna条形码化核小体

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US (2) US12460246B2 (https=)
EP (1) EP3899042A4 (https=)
JP (1) JP2022515221A (https=)
CN (1) CN113508180A (https=)
AU (1) AU2019403351A1 (https=)
CA (1) CA3124482A1 (https=)
WO (1) WO2020132388A1 (https=)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113439121A (zh) * 2019-02-13 2021-09-24 普罗拜奥根股份公司 具有增强的插入位点选择特性的转座酶

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2022056309A1 (en) * 2020-09-11 2022-03-17 Fred Hutchinson Cancer Research Center Improved high efficiency targeted in situ genome-wide profiling
EP4237850A4 (en) * 2020-11-02 2024-09-18 Epicypher, Inc. IMPROVED ASSAYS FOR QUANTIFYING CHROMATIN TARGETS USING ANTIBODY-TARGETED ENZYMATIC DIGESTION
WO2024208912A1 (en) * 2023-04-03 2024-10-10 Belgian Volition Srl Recombinant nucleosome materials
WO2025083285A1 (en) * 2023-10-20 2025-04-24 Norwegian University Of Science And Technology (Ntnu) Methods and products for use in genome-wide chromatin profiling

Citations (6)

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Publication number Priority date Publication date Assignee Title
WO2014205296A1 (en) * 2013-06-21 2014-12-24 The Broad Institute, Inc. Methods for shearing and tagging dna for chromatin immunoprecipitation and sequencing
US20150197801A1 (en) * 2012-06-06 2015-07-16 The Trustees Of Princeton University Dna barcoding of designer mononucleosome and chromatin array libraries for the profiling of chromatin readers, writers, erasers, and modulators thereof
WO2016161314A1 (en) * 2015-04-03 2016-10-06 Dana-Farber Cancer Institute, Inc. Chromatin remodeling activity assays and uses thereof
CN107002289A (zh) * 2014-02-03 2017-08-01 芝加哥大学 用于定量评价dna‑蛋白质复合物密度的组合物和方法
US20180217161A1 (en) * 2017-01-27 2018-08-02 Covaris, Inc. Generation of cfdna reference material
WO2018172726A1 (en) * 2017-03-20 2018-09-27 Blacktrace Holdings Limited Single cell dna sequencing

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SG11201807117WA (en) * 2016-02-23 2018-09-27 Dovetail Genomics Llc Generation of phased read-sets for genome assembly and haplotype phasing

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20150197801A1 (en) * 2012-06-06 2015-07-16 The Trustees Of Princeton University Dna barcoding of designer mononucleosome and chromatin array libraries for the profiling of chromatin readers, writers, erasers, and modulators thereof
WO2014205296A1 (en) * 2013-06-21 2014-12-24 The Broad Institute, Inc. Methods for shearing and tagging dna for chromatin immunoprecipitation and sequencing
CN107002289A (zh) * 2014-02-03 2017-08-01 芝加哥大学 用于定量评价dna‑蛋白质复合物密度的组合物和方法
WO2016161314A1 (en) * 2015-04-03 2016-10-06 Dana-Farber Cancer Institute, Inc. Chromatin remodeling activity assays and uses thereof
US20180217161A1 (en) * 2017-01-27 2018-08-02 Covaris, Inc. Generation of cfdna reference material
WO2018172726A1 (en) * 2017-03-20 2018-09-27 Blacktrace Holdings Limited Single cell dna sequencing

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
JAN BEDNAR 等: "Structure and dynamics of a 197 bp nucleosome in complex with linker histone H1", MOLECULAR CELL, vol. 66, no. 3, pages 385 *
PETER J SKENE 等: "An efficient targeted nuclease strategy for high-resolution mapping of DNA binding sites", ELIFE, 16 January 2017 (2017-01-16), pages 1 *

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113439121A (zh) * 2019-02-13 2021-09-24 普罗拜奥根股份公司 具有增强的插入位点选择特性的转座酶
CN113439121B (zh) * 2019-02-13 2024-12-06 普罗拜奥根股份公司 具有增强的插入位点选择特性的转座酶

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Publication number Publication date
US12460246B2 (en) 2025-11-04
EP3899042A4 (en) 2022-10-12
AU2019403351A1 (en) 2021-08-12
US20220042074A1 (en) 2022-02-10
EP3899042A1 (en) 2021-10-27
CA3124482A1 (en) 2020-06-25
JP2022515221A (ja) 2022-02-17
WO2020132388A1 (en) 2020-06-25
US20260035731A1 (en) 2026-02-05

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