CN113493489A - 具有醒酒功能的多肽scgh及其用途 - Google Patents

具有醒酒功能的多肽scgh及其用途 Download PDF

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CN113493489A
CN113493489A CN202110781160.4A CN202110781160A CN113493489A CN 113493489 A CN113493489 A CN 113493489A CN 202110781160 A CN202110781160 A CN 202110781160A CN 113493489 A CN113493489 A CN 113493489A
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张玉
王伟
王君虹
李雪
孙素玲
白丽萍
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Abstract

本发明属于生物技术领域，特别涉及一个具有激活乙醇脱氢酶和乙醛脱氢酶活性的多肽SCGH，该多肽的氨基酸序列为：Ser‑Cys‑Gly‑His。多肽SCGH能用于制备醒酒产品。

Description

具有醒酒功能的多肽SCGH及其用途
技术领域
本发明属于生物技术领域，特别涉及一个具有激活乙醇脱氢酶和乙醛脱氢酶活性的多肽。
背景技术
酒在我国已经有近千年的历史，也形成了独具特色的酒文化。但饮酒过量或者是经常饮酒则可对人体健康造成一定影响，大量的酒精摄入能刺激食道与胃粘膜，导致胃肠疾病，肝功能代谢障碍，导致肝病变等。过量的酒精进入人体血液循环系统后，还会影响人体神经系统，造成感觉神经麻搏、小脑功能障碍及视神经障碍等。酒精在人体内的分解代谢主要通过肝脏的酶系统进行氧化代谢，酒精进入肝脏通过乙醇脱氢酶脱掉乙醇两个氢原子，使乙醇分解变成乙醛，乙醛进一步通过乙醛脱氧酶氧化为乙酸，最终进入三羧酸循环分解为二氧化碳和水。因此加快肝脏中乙醇的氧化代谢对于醒酒具有重要作用。
目前，市场上很多解酒产品大多是化学制品，具有一定的毒副作用。近几年有研究发现大豆肽、玉米肽等多肽产品可激活乙醇脱氢酶的活性，具有醒酒的作用。由于多肽属天然产品，吸收迅速发挥效果快、无毒副作用，具有较广阔的应用前景。
发明内容
本发明要解决的技术问题是提供一种具有醒酒功能的多肽及其用途。
为了解决上述技术问题，本发明提供一种从香菇蛋白酶解液中鉴定出的多肽SCGH，该多肽的氨基酸序列为：Ser-Cys-Gly-His。
本发明的多肽SCGH可通过香菇蛋白酶解液分离制备，也可由多肽合成公司通过化学合成制备。
本发明提供的多肽具有较强的乙醇脱氢酶和乙醛脱氢酶激活作用，且具有安全无毒副作用、易于吸收等优点，可用于醒酒食品中，服用方式为口服，用量可参照现有的醒酒肽。
本发明的多肽针对乙醇脱氢酶和乙醛脱氢酶具有激活活性，从而表现出具有醒酒功能的特性。
本发明中所涉及的乙醇脱氢酶激活活性的检测方法：
向96孔酶标板中依次加入pH 8.8的焦磷酸钠缓冲液120μL，加入27mmol/L氧化型辅酶I(NAD+)80μL，体积分数为11.5％的乙醇40μL，一定浓度的样品(对照组加入同体积蒸馏水)50μL。混匀，在25℃条件下温育5min，取出立即加入0.5U/mL的乙醇脱氢酶10μL，混匀后于340nm波长处测定吸光度，每10s读数1次，连续测定5min。以A340nm对时间作图。计算△A340nm/min的增加值，以吸光度每分钟的变化率衡量酶活力。
激活率(％)＝(样品组酶活力-对照组酶活力)/对照组酶活力×100％
本发明中所涉及的乙醛脱氢酶激活活性的检测方法：
向96孔酶标板加入100mmol/L pH9.5的焦磷酸钠缓冲液120μL，加入27mmol/L氧化型辅酶I(NAD+)100μL；加入1mmol/L乙醛10μL；10mM吡唑20μL，加入一定浓度的样品(对照组加入同体积蒸馏水)30μL；混匀，在30℃条件下温育5min；取出立即加入1.5U/mL ALDH 15μL摇匀，于340nm波长处测定吸光度，每1min读数1次，连续测定10min。以吸光度每分钟的变化率衡量酶活力。
激活率(％)＝(样品组酶活力-对照组酶活力)/对照组酶活力×100％
本发明从香菇酶解液中分鉴定得到具有乙醇脱氢酶和乙醛脱氢酶激活作用的多肽，可在醒酒产品中应用。
附图说明
下面结合附图对本发明的具体实施方式作进一步详细说明。
图1为本发明的乙醇脱氢酶和乙醛脱氢酶的激活率对比图。
具体实施方式
下面对本发明的实施例作详细说明：本实施例在以本发明技术方案为前提下进行实施，给出了详细的实施方式和具体的操作过程，但本发明的保护范围不限于下述的实施例。
实施例1
多肽SCGH在3.0mg/mL的浓度下的乙醇脱氢酶和乙醛脱氢酶激活活性：
检测方法：将通过化学合成法获得的此多肽SCGH，进行活性检测(检测方法同上)。此时多肽SCGH浓度为3.0mg/mL。
结果：多肽SCGH在3.0mg/mL时的乙醇脱氢酶激活率为76.8％，乙醛脱氢酶激活率为43.8％。对应图1中的“untreated”。
将3mg/mL四肽SCGH溶液于80℃和100℃放置1h后，再进行活性检测(检测方法同上)，测得的乙醇脱氢酶和乙醛脱氢酶激活率如图1，在高温处理下，活性肽的乙醇脱氢酶和乙醛脱氢酶激活率相对比较稳定，具有较好的稳定性。
实施例2
多肽SCGH在1.5mg/mL的浓度下的乙醇脱氢酶和乙醛脱氢酶激活活性：
检测方法：将通过化学合成法获得的此多肽SCGH，进行活性检测(检测方法同上)。此时多肽SCGH浓度为1.5mg/mL。
结果：多肽SCGH在1.5mg/mL时的乙醇脱氢酶激活率为40.6％，乙醛脱氢酶激活率为24.2％。
通过上述实施例中的活性数据可知，此多肽结构具有乙醇脱氢酶和乙醛脱氢酶激活活性，且该活性未见报道，活性效应与剂量具有相关性，属于新的具有乙醇脱氢酶和乙醛脱氢酶激活活性功能肽，该肽还具有较好的热稳定性。
对比例、将表1所述的多肽，按照上述实验方法进行乙醇脱氢酶和乙醛脱氢酶激活活性测定；所得结果与本发明实施例1所得结果的对比如表1所述。
表1、3.0mg/mL浓度下的乙醇脱氢酶和乙醛脱氢酶激活活性
Figure BDA0003157213640000031
实施例3、醒酒肽的应用
1、按50％的醒酒肽，25％的乳糖，25％微晶纤维素充分混合后，通过压片机进行压片，制造成具有醒酒作用的片剂。
2、饮料：将饮料各成分按以下比例进行混合：蜂蜜6％，醒酒肽为1％，柠檬酸0.1％，抗坏血酸0.02％，水92.88％，制成具有醒酒功能的饮料。
最后，还需要注意的是，以上列举的仅是本发明的若干个具体实施例。显然，本发明不限于以上实施例，还可以有许多变形，比如不同蛋白质来源降解分离所得的SCGH结构及其衍生化结构。本领域的普通技术人员能从本发明公开的内容直接导出或联想到的所有变形，均应认为是本发明的保护范围。
序列表
<110> 浙江省农业科学院
<120> 具有醒酒功能的多肽SCGH及其用途
<160> 1
<170> SIPOSequenceListing 1.0
<210> 1
<211> 4
<212> PRT
<213> 人工序列(Artificial Sequence)
<400> 1
Ser Cys Gly His
1

Claims (3)

1.具有醒酒功能的多肽SCGH，其特征是：该多肽的氨基酸序列为：Ser-Cys-Gly-His。
2.如权利要求1所述的多肽SCGH的用途，其特征是：制备醒酒产品。
3.根据权利要求2所述的多肽SCGH的用途，其特征是：同时具有乙醇脱氢酶激活活性和乙醛脱氢酶激活活性。
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CN114409731B (zh) * 2022-01-05 2023-08-11 浙江省农业科学院 同时具有激活乙醇脱氢酶和乙醛脱氢酶活性的两种多肽

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