CN113491755A

CN113491755A - Combined prescription for treating cervical HPV infection

Info

Publication number: CN113491755A
Application number: CN202110855075.8A
Authority: CN
Inventors: 李颖
Original assignee: Individual
Current assignee: Individual
Priority date: 2021-07-28
Filing date: 2021-07-28
Publication date: 2021-10-12

Abstract

The invention belongs to the field of traditional Chinese medicine preparations, and particularly relates to a combined prescription for treating cervical HPV infection, which comprises honeysuckle liquid and Qinghua powder; the honeysuckle liquid comprises 3-50 g of coptis chinensis, 10-100 g of honeysuckle, 10-50 g of curcuma zedoary, 5-60 g of fructus cnidii, 5-90 g of rhizoma bletillae, 3-90 g of brucea javanica, 10-60 g of folium isatidis, 3-40 g of catechu, 5-50 g of dried alum, 5-60 g of radix sophorae flavescentis, 10-100 g of frankincense, 5-90 g of gallnut, 5-100 g of raw astragalus membranaceus and 3-30 g of liquorice; the blue and white flower powder comprises 5-200 g of indigo naturalis, 10-150 g of honeysuckle, 10-150 g of coptis chinensis, 1-100 g of dragon's blood, 5-150 g of rhizoma bletillae, 10-150 g of catechu, 2-150 g of dried alum and 3-50 g of liquorice. The invention has strong antivirus function, can inhibit the replication and reproduction of viruses, repair local tissues and accelerate the healing of erosion surfaces.

Description

Combined prescription for treating cervical HPV infection

Technical Field

The invention belongs to the field of traditional Chinese medicine preparations, and particularly relates to a combined prescription for treating cervical HPV infection.

Background

Cervical cancer is the most common malignancy of the female reproductive tract, and is also only lower in incidence than breast cancer in women. According to statistics, the number of new cervical cancer cases is about 50 ten thousand every year in the world, wherein the number of new cervical cancer cases in China is about 14 ten thousand every year, which is equivalent to 28% of the total disease rate in the world, and the number of cases dying from the cervical cancer every year is about 2-3 ten thousand, and the incidence rate of the cervical cancer is gradually increased along with the development of social economy and the change of life style of residents, and tends to be younger. For the pathogenesis of cervical cancer, some foreign studies have shown that the most advanced of themThe main reason is HPV infection, and 18 of the HPV genotypes of about 200 have direct relation with the occurrence and development of cervical cancer. Cervical Intraepithelial Neoplasia (CIN), one of the most closely related precancerous lesions of invasive cervical cancer, is usually classified into three stages, i.e., according to the degree of cellular abnormality thereof

CINI, wherein the nucleus of the upper and lower subcutaneous 1/3 layers is enlarged, the proportion of the nucleus and the cytoplasm is slightly increased, the nuclear staining is slightly deepened, the nuclear division image is few, and the polarity of the cells is normal;

CINII, wherein the nucleus of the upper and lower subcutaneous layers 1/3-2/3 is obviously enlarged, the proportion of the nucleus and the cytoplasm is increased, the nucleus is deeply dyed, the number of nucleus division is large, the cell number is obviously increased, and the cell polarity still exists;

CINIII, the pathological cells almost or completely occupy the whole epithelial layer, the cell nucleus is abnormally increased, the proportion of the nucleoplasm is obviously increased, the nuclear shape is irregular, the nuclear staining is deep, the nuclear division is numerous, the cell arrangement is crowded and disordered, and no polarity exists. The occurrence and development process of cervical intraepithelial neoplasia to invasive cervical cancer is progressive and is a continuous process from quantitative change to qualitative change, namely CINI-CINII-CINIIII-carcinoma in situ-early stage invasive carcinoma series change. Therefore, after the organism is infected with HPV, whether the HPV can be eliminated and whether the persistent high-risk HPV infection occurs can be further developed into CIN or invasive cervical carcinoma, and the relation with the immunity of the organism is established. If the body has strong enough immunity, HPV is likely to be eliminated, thereby blocking the canceration development process of CINI-CINII-CIN III-carcinoma in situ-early stage infiltration carcinoma. HR-HPV infection is the most main pathogenic factor of CIN and cervical cancer, about more than 90 percent of CIN have HPV infection, about 20 percent of sexually-living women have HPV infection, but the HPV infection is not persistent and can be naturally resolved without clinical symptoms. For most patients, it is unacceptable to treat the disease by self-healing and waitingThe treatment method is a method that is urgently expected to block the development of diseases through active treatment means such as medicines, physics, operations and the like. In recent years, the continuous infection of HPV causes the recurrence of patients with cervical cancer after receiving treatment. At present, the common clinical treatment methods mainly comprise surgical treatment and chemical treatment, but because early symptoms of cervical cancer are not obvious, most patients are diagnosed at a late stage with poor surgical treatment effect and are mostly combined with chemotherapy. Chemotherapy can cause certain damage to the body of a patient, greatly influences the treatment effect of the patient and brings negative emotion to the patient, so that the research of auxiliary chemotherapy drugs is continuously devoted to the clinic. In recent years, the traditional Chinese medicine has certain advantages in the adjuvant therapy of tumor chemotherapy, including lower toxic and side effects, reduction of adverse reactions of western medicines and the like. VAIN and CIN are frequently found in clinical work, the incidence rate of the VAIN and CIN is obviously lower than that of CIN, VAINI is also called low-grade squamous intraepithelial lesion (LSIL) of vaginal wall, and VAIN II-III is also called high-grade squamous intraepithelial lesion (HSIL) of vaginal wall. VAIN has approximately the same risk factors as CIN, and comprises patients with sexual life and a plurality of sexual partners due to small age, accompanied by bad habit such as smoking, condyloma acuminatum caused by persistent high-risk HPV infection or low-risk HPV infection, or long-term taking of immunosuppressant, wherein the most important risk factors are patients with cervical precancerous lesion or cervical cancer. And many patients infected by HPV virus are anxious and worried about the progress of low-grade lesion into high-grade lesion or canceration, doctors are required to treat the disease in a dispute, even frequent and close colposcopy is carried out, and the spirit and the body cause certain trauma, but at present, no clear guide or expert consensus is provided for the treatment of the vaginal wall lesion, and the evaluation on the aspects of curative effect, side effect, cost performance and the like is carried out, so that the finding of a proper medicine is always carried out by the clinician. In recent years, the incidence rate of TCT and HPV is increasing due to the emphasis of gynecologists and the increase of examination methods. VAIN can be primary lesion or secondary lesion, has no symptoms in most patients, and is multifocal and tiny in focus and easy to be missed. There is no specific discussion of cervical HPV infection in traditional medicine. But rather according to cervical HPV infectionThe clinical symptoms of the diseases include that the cervical HPV infection is classified into leukorrhagia or five-color leukorrhea, and the like, wherein the leucorrhea is more, less and alternate yellow and white or yellow like pus, and sometimes the leucorrhea with red and white color is shown. It is also indicated that the disease is closely related to the spleen, liver, kidney and ren vessels, and the disease can be classified into deficiency syndrome, excess syndrome and intermingled deficiency and excess. Deficiency is mainly due to deficiency of spleen, liver and kidney, while excess is mainly due to damp-toxicity. Clinically, the deficiency and excess are often accompanied by miscellaneous diseases. According to clinical manifestations, the traditional Chinese medicine considers that the cervical cancer belongs to the categories of five-color strip, vulval ulcer, metrorrhagia and metrostaxis, menstruation recurrence and the like in the traditional Chinese medicine, for example, Sun Simiao indicates in the 'Qianjin essentials' of Sun Simiao: "Furen metrorrhagia and metrostaxis … … swelling in yin with sores". According to the pathogenesis and mechanism pointed out by HR-HPV modern research, the human body can be regarded as exogenous pathogenic infection, and the diseases are mainly caused by premature marriage and childbirth, sexual disorder (cleaning), improper diet or depressed emotion, which cause dysfunction of internal organs of the human body, imbalance of qi and blood of the body and deficiency of vital qi. As reported in Huang Di Nei Jing, the healthy qi is always involved in the interior and the pathogenic factors are not always dry, so qi is always deficient. "Yi Zong Bi Shu" (essential reading of medicine) means that the accumulation is due, the healthy qi is deficient, and then pathogenic qi is recumbent. "Hua Tuo Zhong Zai Jing (Hua Tuo Zhong Jing) means that all the five zang organs fail to check true qi to cause pathogenic qi, so that all the diseases are born. "this indicates that qi stagnation and blood stasis due to the deficiency of healthy qi and the inability to defend the pathogenic factors, and the external pathogenic factors driving directly into the uterus and targeting the uterus gate gradually lead to the development of pre-cervical lesion and even cervical cancer. The traditional Chinese medicine considers that the occurrence, development and prognosis of the disease are determined by the strength of the struggle between vital qi and pathogenic factors of the body. Modern medicine indicates that cervical cancer is a type of malignant tumor which can be developed into cancer by preventing and treating precancerous lesions, so that the aim of treating cervical cancer is achieved by preventing and treating the precancerous lesions based on the concept of treating the precancerous lesions.

Disclosure of Invention

The invention aims to overcome the defects of the prior art and provides a combined prescription for treating cervical HPV infection, which has stronger antiviral effect, can inhibit the replication and propagation of viruses, repair local tissues and accelerate the healing of erosion surfaces.

In order to solve the technical problem, the invention is realized as follows:

a combined prescription for treating cervical HPV infection comprises honeysuckle liquid and Qinghua powder in parts by weight;

the honeysuckle liquid comprises 3-50 g of coptis chinensis, 10-100 g of honeysuckle, 10-50 g of curcuma zedoary, 5-60 g of fructus cnidii, 5-90 g of rhizoma bletillae, 3-90 g of brucea javanica, 10-60 g of folium isatidis, 3-40 g of catechu, 5-50 g of dried alum, 5-60 g of radix sophorae flavescentis, 10-100 g of frankincense, 5-90 g of gallnut, 5-100 g of raw astragalus membranaceus and 3-30 g of liquorice;

the honeysuckle flower powder comprises 5-200 g of indigo naturalis, 10-150 g of honeysuckle, 10-150 g of coptis chinensis, 1-100 g of dragon's blood, 5-150 g of rhizoma bletillae, 10-150 g of catechu, 2-150 g of dried alum and 3-50 g of liquorice.

As a preferable scheme, the honeysuckle flower liquid is prepared from 3g of coptis chinensis, 10g of honeysuckle flower, 10g of curcuma zedoary, 5g of fructus cnidii, 5g of rhizoma bletillae, 3g of brucea javanica, 10g of folium isatidis, 3g of catechu, 5g of dried alum, 5g of radix sophorae flavescentis, 10g of frankincense, 5g of gallnut, 5g of raw astragalus membranaceus and 3g of liquorice.

Further, the honeysuckle flower powder comprises 5g of indigo naturalis, 10g of honeysuckle, 10g of coptis chinensis, 1g of dragon's blood, 5g of rhizoma bletillae, 10g of catechu, 2g of dried alum and 3g of liquorice.

Furthermore, the honeysuckle flower liquid comprises 15g of coptis chinensis, 40g of honeysuckle, 25g of curcuma zedoary, 30g of fructus cnidii, 45g of rhizoma bletillae, 45g of brucea javanica, 50g of folium isatidis, 25g of catechu, 25g of dried alum, 40g of radix sophorae flavescentis, 50g of frankincense, 45g of gallnut, 50g of raw astragalus membranaceus and 25g of liquorice.

Further, the honeysuckle flower powder comprises 100g of indigo naturalis, 70g of honeysuckle, 75g of coptis chinensis, 75g of dragon's blood, 70g of rhizoma bletillae, 80g of catechu, 75g of dried alum and 25g of liquorice.

Furthermore, the honeysuckle flower liquid comprises 50g of coptis chinensis, 100g of honeysuckle, 50g of curcuma zedoary, 60g of fructus cnidii, 90g of rhizoma bletillae, 90g of brucea javanica, 60g of folium isatidis, 40g of catechu, 50g of dried alum, 60g of radix sophorae flavescentis, 100g of frankincense, 90g of gallnut, 100g of raw astragalus membranaceus and 30g of liquorice.

Further, the honeysuckle flower powder comprises 200g of indigo naturalis, 150g of honeysuckle, 150g of coptis chinensis, 100g of dragon's blood, 150g of rhizoma bletillae, 150g of catechu, 150g of dried alum and 50g of liquorice.

The medicine composition has the following pharmacological actions: indigo naturalis: bitter, salty and cold herbs enter liver, heart, lung and stomach meridians. Clear heat and remove toxicity, cool blood and purge liver fire. The pharmacological action is as follows: 1. the anti-tumor effect is as follows: indigo naturalis and indirubin are effective in treating chronic granulocytic leukemia. The indirubin has certain inhibitory effect on W256 solid tumor of rat, Lewis lung cancer of mouse and breast cancer. 2. Impact on immune function: indigo naturalis and indirubin have certain promoting effect on macrophage function of abdominal cavity of mice. It also has promoting effect on macrophage phagocytosis of tumor-bearing mice. 3. The antibacterial effect is as follows: the indigo naturalis ethanol leachate 0.5g/ml has effect in inhibiting Bacillus anthracis, Bacillus pneumoniae, Shigella shigella, Vibrio cholerae, Staphylococcus aureus and Staphylococcus albus. Indigo naturalis component tryptanthrin has inhibitory effect on Microsporum lanosum, Trichophyton mentagrophytes, Trichophyton rubrum, and Epidermophyton floccosum. 4. Other functions are as follows: the indigo blue has a certain protection effect on mouse liver injury caused by carbon tetrachloride. 5. In vivo process: the peak is reached in 12 hours, and the slow decline is kept for a longer time after 1 day. The distribution in the body is highest in liver, gallbladder, stomach and intestine. The bioavailability is 46.48%. Modern applications are as follows: tumors, psoriasis, canker sores, impetigo, exfoliative dermatitis, otitis externa, chronic leg ulcers, dry socket. Coptis chinensis: bitter and cold herbs enter heart, stomach, large intestine, liver and gallbladder meridians to clear heat and dry dampness, purge fire and remove toxicity. The pharmacological action is as follows: 1. pathogenic microorganisms are: (1) and (3) antibiosis: berberine has antibacterial spectrum; (2) resisting viruses; (3) resisting protozoa; 2. anti-inflammatory and analgesic effects; 3. anti-arrhythmic; 4. inhibiting platelet aggregation; 5. the function of reducing blood pressure: berberine can reduce arterial blood pressure, especially has remarkable diastolic pressure; 6. and (3) reducing blood sugar: berberine; 7. antioxidation; 8. effect on smooth muscle: the berberine has obvious excitation effect on isolated guinea pig and cat uterus. 9. Antiulcer effect: (Huanglian SHI Tang, Sanhuang Xiexin Tang) has obvious effects of resisting stress ulcer and inhibiting gastric secretion. 10. Antitoxic action: has obvious antagonism on various germs and toxins 11 and immunity enhancement function: reducing the weight of thymus and spleen of a rat, improving the phagocytosis rate and phagocytosis index of macrophages in abdominal cavity of the mouse, promoting the generation of antibody, and enhancing the content of serum lysozyme. 12. Other functions are as follows: and (3) anticancer: berberine has anticancer activity, and can inhibit respiration of cancer cells in pleural effusion, inhibit utilization of hydroxylamine by cancer cells to inhibit formation of purine and nucleic acid, and block cancerogenic substances from having potential cancerogenic cell effect. Modern applications are as follows: chronic cholecystitis, duodenitis, burn, diabetes, and infection after anorectal operation. Brucea javanica: bitter and cold has the pharmacological actions of clearing heat and detoxicating with small toxicity, preventing malaria, stopping dysentery and corroding excrescence: 1. resisting pathogenic bacteria such as amoeba; 2. the anti-tumor effect is as follows: 10% oleum fructus Bruceae can kill hepatocarcinoma cell; 3. enhancing the immunity: the oleum fructus Bruceae has effects of promoting spleen weight, hemolytic plaque number, phagocytic function of abdominal cavity macrophage and proliferation of bone marrow hematopoietic stem cell after cobalt irradiation; 4. effects on the cardiovascular and cerebrovascular System: lowering blood pressure; 5. dose for smooth muscle stimulation: orally administered, 5-15 granules are swallowed each time, 3 times in 1 day, and the medicine is coated with arillus longan or encapsulated for oral administration, or administered after sub-deoiling and made into pill or tablet; it should not be taken in decoction. External use: proper amount. Modern applications are as follows: 1. tumor: the cervical cancer is treated by local injection of 5 percent and 10 percent of oleum fructus bruceae and matching with oral administration and external application of traditional Chinese medicines; 2. amebic dysentery; 3. ulcerative colitis; 4. condyloma acuminata: dipping 100% oleum fructus Bruceae with cotton swab, and uniformly applying to wart for 2 times per day and 3-15 days to cure. Zedoary turmeric: pungent and bitter with warm property entering liver and spleen meridians, promoting qi and blood circulations, removing food stagnation and relieving pain. The pharmacological action is as follows: 1. the anticancer effect is as follows: zedoary turmeric oil solvent, curcumenol, zedoary turmeric diketone, etc. are effective anticancer components, beta-elemene is also effective anticancer component, and the inhibition rate of mouse cervical carcinoma V14 reaches 77.13%. The zedoary turmeric diketone has effects of inhibiting mouse sarcoma, cervical cancer, and ehrlich ascites carcinoma. The volatile oil preparation is systemically used for treating ovarian cancer, malignant lymphoma, lung cancer and liver cancer, and has certain curative effect and no obvious side effect. The experimental animal is inoculated with the curcuma zedoaria to treat the cancer vaccine, the immune function of the animal can be obviously enhanced, the anti-cancer oral liquid has important effects on inhibiting and killing tumor cancer cells and preventing the tumor cancer cells from diffusing and transferring, and the curcuma zedoaria compound preparation 'strengthening body resistance and resisting cancer oral liquid' also has obvious anti-cancer effect. 2. Anti-inflammatory, antibacterial 3, anti-early pregnancy: the product has remarkable effects of resisting implantation and early pregnancy, and can make animal corpus luteum atrophy, cytolymph dissolve, gradually replace connective tissue, no decidua reaction occurs to endometrium, secretion period is inhibited, and decidua and embryo drop in uterine cavity. 4. Protecting liver and resisting thrombosis; 5. in vivo process: the blood concentration can be detected in 5 minutes, the peak blood concentration can be reached in 15 minutes, the blood concentration can be maintained for 1 hour, and the half-life period is about 11.5 hours. Liver and kidney are distributed most, and blood-brain barrier is permeable, so that urine is mainly excreted, bile is also excreted, and liver and intestine circulation is caused. Modern applications are as follows: 1. tumor: the curcuma zedoary has wide anti-tumor range and is clinically used for treating cervical cancer, ovarian cancer, vulvar cancer, malignant lymphoma, skin cancer and the like. Has good curative effect on cervical cancer, and has prevention and treatment effects on leucopenia caused by radioactivity. 2. Cervical erosion; 3. ischemic cerebrovascular diseases, etc.; 4. urinary calculi; 5. the early pregnancy has certain effect of resisting the early pregnancy.

Honeysuckle flower: sweet and cold in nature, clear heat and remove toxicity, cool and disperse wind-heat. The pharmacological action is as follows: 1. antibacterial and antiviral effects: the antibacterial spectrum is wide, and the main effective components are as follows: chlorogenic acid compounds; 2. anti-inflammatory, antipyretic and antiallergic effects; 3. impact on immune function: the decoction promotes the phagocytic function of the white blood cells; 4. central excitability: an intensity of caffeine 1/6; 5. reducing blood lipid; 6. anti-fertility function: the ethanol extraction of honeysuckle flower from honeysuckle flower can obviously reduce the plasma progesterone of the early pregnant rat, but can be completely cancelled by exogenous progesterone and HCG. 7. Honeysuckle water: has obvious toxic effect on sarcoma and ehrlich ascites carcinoma. Modern applications are as follows: acute infectious diseases, infectious hepatitis, infantile diarrhea, leptospirosis. Dragon's blood: sweet, salty and mild, promoting blood circulation to dissipate blood stasis, stopping bleeding, promoting granulation and healing wound. The pharmacological action is as follows: 1. effects on the blood system: (1) hemostatic (2) antithrombotic (3) effects on blood rheology; 2. effects on the cardiovascular system; 3. an analgesic effect; 4. anti-inflammatory action: has effects in relieving swelling, reducing purulent secretion, astringing, and promoting wound healing; 5. and (5) bacteriostasis. Modern applications are as follows: upper gastrointestinal hemorrhage, dysmenorrhea, menorrhagia, fracture, soft tissue injury, spermatorrhea, and old myocardial infarction. Toxic and side effects: has no obvious toxicity. Xue Jie is pungent and hot with dry and drastic property, so it should be used with cautions for yin deficiency and blood heat. The oral or external application of dragon's blood can cause individual contact allergy.

And (3) common bletilla pseudobulb: bitter, sweet, astringent, slightly cold, astringing to stop bleeding, subduing swelling and promoting granulation. The pharmacological action is as follows: 1. Hemostasis effect: has the functions of obviously shortening the blood coagulation time and the prothrombin time, and the hemostasis effect of the rhizoma bletillae is related to the physical hemostasis effect, the inhibition of fibrinolysis and the enhancement of the activity of platelet III silver. 2. Antiulcer effect: can enhance the function of mucosal defense factors. 3. The anticancer effect is as follows: the product has certain inhibitory effect on mouse Ehrlich ascites carcinoma, cervical cancer 14, sarcoma S180, rat Wacker carcinoma, and hepatocarcinoma. The bletilla striata is used as the vascular embolization agent for tumor interventional therapy, can block the effective blood supply of the tumor in a large area, can prevent the revascularization of the tumor, and can obviously prolong the survival rate of tumor patients. 4. The antibacterial effect is as follows: in vitro tests show that the compound has obvious inhibition effect on human type tubercle bacillus and certain inhibition effect on gram-positive bacteria such as staphylococcus, streptococcus and the like. The usage and dosage are as follows: decocting: 6-15g, ground into powder for oral administration, 2-5g for each time, and proper dosage is applied externally. Modern applications are as follows: gastrointestinal hemorrhage, gastric ulcer, anal fissure, anal fistula, chyluria.

Fructus cnidii: pungent, bitter and warm in property, with the action of mild toxicity to warm kidney and strengthen yang, dry dampness, dispel wind and kill parasites. The pharmacological action is as follows: 1. anti-arrhythmic effect; 2. antimicrobial, parasitic effects; 3. a sex hormone-like action; 4. antiallergic action: fructus Cnidii acetone extract has strong antihistamine effect. Can obviously antagonize contraction of intestinal muscles caused by histamine, chronic reaction substances and 2 percent of egg white; inhibiting passive cutaneous anaphylaxis in mice; 5. anti-mutagenic effect; 6. local anesthesia is effective. Modern applications are as follows: trichomonas vaginitis, tinea pedis complications, scabies nodules and lichen vulvus. Catechu: bitter, astringent and slightly cold herbs enter heart and lung meridians to astringe dampness, promote tissue regeneration and heal wound. 1. The antibacterial effect is as follows: the water decoction has antibacterial effect on Staphylococcus aureus, Pseudomonas aeruginosa, diphtheria bacillus, Bacillus proteus, Bacillus dysenteriae, and Bacillus typhi. On the culture medium, the product can be sterilized within 24 hours by using 10% solution. 2. Impact on the circulation system; 3. protecting liver, promoting function of gallbladder, lowering blood sugar and blood lipid; 5. relieving diarrhea; 6. other functions are as follows: the contraceptive effect and the decoction can inactivate influenza virus in vitro, 20 percent of the decoction can damage ascites cancer cells in vitro, and catechu tannin can inhibit experimental vesical calculus formation of rats. Modern applications are as follows: cervicitis: catechu and alum each 10g, borneol 1g are ground into powder and mixed into paste, 2g of cotton ball plaster is put on the cervical erosion face for 24 hours and taken out, one time is taken for 3-4 days, and 10 times of a treatment course are taken. Cervical erosion: antiphlogistic and granulation promoting powder (dried alum, gallnut, honeysuckle, catechu and liquorice).

Dried alum: calcining clean Alumen with illumination to crisp. Sour, astringent and cold in nature and taste. Meridian tropism enters lung, spleen, liver and large intestine meridians. The functions are mainly used for externally detoxifying, killing insects, eliminating dampness and relieving itching; orally administered to stop bleeding and check diarrhea, dispel wind-phlegm. The external treatment is used for eczema, mange and purulent pus in purulent otitis media; it is orally administered for treating chronic diarrhea, hematochezia, metrorrhagia, epilepsy, and mania. Dried alum astringes dampness to heal wound, stops bleeding and removes putridity. Can be used for treating eczema, purulent vulva, epistaxis, gingival hemorrhage, and nasal . Licorice root: sweet, tonifying spleen and replenishing qi, clearing away heat and toxic material, expelling phlegm and arresting cough, relieving spasm and pain, and harmonizing the drugs. The pharmacological action is as follows: corticoid-like action, anti-inflammatory and antiallergic action, action on immune function, detoxifying action, action on digestive system, influence on lipid metabolism, pain relieving, and anticonvulsive action. Modern applications are as follows: chronic adrenocortical insufficiency (addison's disease), peptic ulcer, cold injury, gastrocnemius spasm.

Flavescent sophora root: bitter and cold herbs can clear heat and dry dampness, kill parasites and induce diuresis. Can be used for treating dysentery, hematochezia, jaundice, anuria, leucorrhea with red and white discharge, pruritus vulvae, eczema, skin pruritus, scabies, tinea, leprosy, etc. It can be used for treating trichomonas vaginitis by external application. The pharmacological action is as follows: resisting pathogenic microorganisms; the anticancer effect is as follows: the physiological active substances such as the lightyellow sophora root and the like have wide effects on the anti-tumor efficacy; has the effects on cardiovascular diseases: resisting arrhythmia, reducing blood lipid, and lowering blood pressure; anti-inflammatory effects; protecting liver and kidney, brain, and gastric mucosa, and relieving pain and central nervous system. Frankincense: pungent, bitter and warm herbs can activate blood and relieve pain, resolve swelling and promote tissue regeneration. Can be used for treating thoracic obstruction, cardialgia, epigastralgia, dysmenorrhea, amenorrhea, puerperal blood stasis, abdominal mass, rheumatalgia, spasm of muscles and tendons, traumatic injury, carbuncle, swelling, and pyocutaneous disease. The pharmacological action is as follows: resisting ulcer of stomach, duodenum and oral mucosa; anti-inflammatory, analgesic, and repercussive; has effects in reducing cholesterol, resisting tumor, terminating pregnancy, and improving memory.

Gallnut: sour, astringent and cold. Astringe lung to reduce fire, astringe intestine to check diarrhea, stop bleeding, astringe dampness to heal wound. Can be used for treating chronic cough due to lung deficiency, chronic diarrhea, spontaneous perspiration, night sweat, diabetes, hematochezia, hemorrhoid, traumatic hemorrhage, carbuncle, suppurative sore, and skin wet rot. The pharmacological action is as follows: astringing, antibacterial, antiviral, antifertility, and antiulcer effects; detoxicating, protecting liver, reducing blood sugar, resisting oxidation, resisting aging, and resisting tumor. Raw astragalus root: sweet and mild in nature, tonifying qi and raising yang, consolidating superficial resistance and arresting sweating, inducing diuresis and removing edema, promoting production of body fluid and nourishing blood, activating stagnancy and removing obstruction of qi, expelling toxin and pus, healing sore and promoting granulation. The pharmacological action is as follows: enhancing immunity, blood system, cardiovascular system, and urinary system, resisting aging, protecting liver, resisting virus, resisting tumor, and relieving inflammation. Compatibility of the flower connecting liquid: monarch: coptis chinensis and honeysuckle: clearing away heat and toxic materials, and eliminating dampness; minister: zedoary, frankincense and brucea javanica: clearing away heat and toxic materials, relieving swelling and pain, and promoting granulation; flavescent sophora root, dyers woad leaf: clearing away heat and toxic materials, and eliminating dampness; and (2) adjuvant: fructus cnidii and dried alum: detoxify and kill parasites, dry dampness and relieve itching; raw astragalus, gallnut, bletilla and catechu: expelling toxin and pus, healing sore and promoting granulation, and stopping bleeding; the following steps are performed: licorice root: harmonize the effects of the drugs, clear heat and remove toxicity. The efficacy of the Qinghua powder is as follows: clearing away heat and toxic material, removing necrotic tissue and promoting granulation. Eliminating HPV virus and promoting the healing of local erosion surface.

Coptis root, honeysuckle flower, flavescent sophora root and dyers woad leaf have the functions of clearing away heat and toxic material and eliminating dampness, and have strong antiviral action. Can inhibit replication and reproduction of virus, and can prevent synthesis of HPVDNA protein and stimulate and improve skin immunity. Has broad-spectrum antiviral and antibacterial effects. Brucea javanica can selectively interfere energy metabolism of tumor cells, so that HPV parasitized in the cells is destroyed, replication and reproduction of the HPV are inhibited, the brucea javanica contains a plurality of components such as grease, alkaloid, bitter principle and the like, the brucea javanica selectively breaks down mitochondria and membranous structures of the tumor cells, so that the tumor cells are necrotized and degenerated, the cellular immunity and humoral immunity functions of a human body can be adjusted, and normal cells are not damaged. The fructus Cnidii has the function of exfoliating keratinocytes, so that virus particles in epithelial cells are exfoliated together with host cells before propagation and release are completed, thereby achieving the effect of removing viruses. Raw zedoary, frankincense, astragalus, gallnut, bletilla, catechu and dragon's blood: expelling toxin and pus, healing sore and promoting granulation, and stopping bleeding. The liquorice coordinates the effects of the various medicines and simultaneously plays the roles of clearing heat and removing toxicity. The whole formula is reviewed to achieve the effects of clearing heat and detoxicating, reducing swelling, eliminating HPV virus infection, reducing purulent secretion, astringing and promoting tissue regeneration. The HPV virus is removed, and meanwhile, local tissues are repaired, and the healing of the erosion surface is accelerated.

The invention has strong antivirus function, can inhibit the replication and reproduction of viruses, repair local tissues and accelerate the healing of erosion surfaces.

The present invention will be further described with reference to the following embodiments. The scope of the invention is not limited to the following expressions.

Detailed Description

Example 1

The combined prescription for treating cervical HPV infection comprises honeysuckle liquid and Qinghua powder in parts by weight; the flower connecting liquid comprises the following components: 3g of coptis chinensis, 10g of honeysuckle, 10g of curcuma zedoary, 5g of fructus cnidii, 5g of rhizoma bletillae, 3g of brucea javanica, 10g of folium isatidis, 3g of catechu, 5g of dried alum, 5g of radix sophorae flavescentis, 10g of frankincense, 5g of gallnut, 5g of raw astragalus membranaceus and 3g of liquorice; the honeysuckle flower powder comprises 5g of indigo naturalis, 10g of honeysuckle, 10g of coptis chinensis, 1g of dragon's blood, 5g of rhizoma bletillae, 10g of catechu, 2g of dried alum and 3g of liquorice.

The honeysuckle liquid is a traditional decoction method, is prepared into a traditional Chinese medicine decoction, has large dosage each time, is difficult to store and is inconvenient to carry. Further develop and extract the process, concentrate the formulation, improve the potency and facilitate preserving.

The Qinghua powder is prepared by finely grinding traditional Chinese medicine decoction pieces to form powder to be sprayed on the surface of an affected part of the cervix or to be filled into capsules so as to be convenient for a patient to apply the medicine by himself. The medicine can be further developed and filled into a vaginal medicine applicator, so that the melting process of the capsule is omitted, and the medicine can better act on the affected part.

Example 2

The combined prescription for treating cervical HPV infection comprises honeysuckle liquid and Qinghua powder in parts by weight; the honeysuckle liquid comprises 15g of coptis chinensis, 40g of honeysuckle, 25g of curcuma zedoary, 30g of fructus cnidii, 45g of rhizoma bletillae, 45g of brucea javanica, 50g of folium isatidis, 25g of catechu, 25g of dried alum, 40g of radix sophorae flavescentis, 50g of frankincense, 45g of gallnut, 50g of raw astragalus mongholicus and 25g of liquorice; the honeysuckle flower powder comprises 100g of indigo naturalis, 70g of honeysuckle, 75g of coptis chinensis, 75g of dragon's blood, 70g of rhizoma bletillae, 80g of catechu, 75g of dried alum and 25g of liquorice.

The preparation method is the same as example 1.

Example 3

The combined prescription for treating cervical HPV infection comprises honeysuckle liquid and Qinghua powder in parts by weight; the honeysuckle liquid comprises 50g of coptis chinensis, 100g of honeysuckle, 50g of curcuma zedoary, 60g of fructus cnidii, 90g of rhizoma bletillae, 90g of brucea javanica, 60g of folium isatidis, 40g of catechu, 50g of dried alum, 60g of radix sophorae flavescentis, 100g of frankincense, 90g of gallnut, 100g of raw astragalus mongholicus and 30g of liquorice; the honeysuckle flower powder comprises 200g of indigo naturalis, 150g of honeysuckle, 150g of coptis chinensis, 100g of dragon's blood, 150g of rhizoma bletillae, 150g of catechu, 150g of dried alum and 50g of liquorice.

The preparation method is the same as example 1.

The application of the honeysuckle liquid is started 3 days after every month of menstruation of the patient, and a treatment scheme is given according to the condition of the patient: 1. the medicine application time is as follows: (1) continuously applying the medicine for three days, and resting for 4 days (self-sitting bath for washing vulva by diluting with the honeysuckle liquid 1: 10). (2) The medicine is applied every other day every week (during the period of no application, the flower liquid is diluted with 1:10, and then the vulva is washed by hip bath by oneself). 2. A medicine feeding step: (1) and (3) disinfecting the vulva, and carrying out wet application on the vulva for 10 minutes by using the dilution of the honeysuckle liquid with the ratio of 1: 10. (2) 10ml of the flower connecting liquid is soaked in a sterile cotton ball and is applied to the surface of the cervical canal, the cervix and the vaginal wall for 10 minutes in a wet mode. (3) Aiming at a patient infected by simple HPV, 10ml of the flower connecting liquid is used for infiltrating sterile gauze to wrap cotton balls, the cotton balls are stuffed on the surface of a cervix, and the gauze is left at the vaginal orifice, so that the patient can take out the cotton balls conveniently after 2-5 hours. Aiming at HR-HPV infection accompanied with cervical epithelial lesion (AUSCS, LSIL or focal HSIL), spraying blue-and-white powder on the surface of cervix, then soaking 10ml of a cotton ball wrapped by sterile gauze with a connecting flower solution, plugging the cotton ball on the surface of cervix, and leaving the gauze head on the vaginal orifice, so that a patient can take out the gauze automatically after 2-5 hours. (4) The application time of the method is 3-6 months, and the above-mentioned medicine is repeatedly examined at 1, 3, 6, 9, 12 and 24 months after stopping application of the medicine, and TCT, HPV, colposcope and cervical biopsy are taken if necessary according to slow disease condition.

The clinical observation of the LIANHUA liquid and the QINGHUASAN for treating cervical HR-HPV infection is 12 months, 50 patients are treated systematically and are reviewed, 20 patients are reviewed at present twice, and 15 patients turn to negative, wherein 3 patients are positive for HPV16, and 1 patient is positive for HPV 18. (for 16 and 18 types of infection with cervical epithelial lesion, taking biopsy again through colposcope during double examination, recovering 3 cases, improving 1 case and giving a second course of treatment.)

[ case 1] female 23 years old first visit time: 24 days 6 month in 2020

The main complaints are: the vulva is uncomfortable for one month.

And (3) gynecological internal diagnosis: the vulva and vagina develop normally, the secretion is normal, the cervical erosion degree II, the blood contact (+), the uterus and the double annexes are not obviously abnormal.

Pregnancy history: and (5) unmarring and non-pregnant.

Auxiliary inspection: HPV: high-risk HPV type 51, type 52 positive TCT: LSIL. Colposcope: the acetic acid-white epithelium is evident, and the mosaic is evident.

Pathological biopsy: cervical 3-point LSIL, focal HSIL5-6, 9, 11-point LSIL

Immunohistochemistry: the 3 points are in agreement with LSIL P16 mottle (+) Ki67 that positive cells are distributed in epithelium 2/3.

Consultation in Liaoning province tumor hospital: pathological diagnosis is consistent with LSIL, P16 is partially positive, except for HSIL. After the traditional Chinese medicine is used in a hospital, the clinical symptoms of the honeysuckle liquid and the blue and white flower powder are obviously improved after the traditional Chinese medicine is used for 3 months. The cervical HPV is negative in the reexamination, TCT is the change of inflammatory reactivity, the colposcopy shows that the cervical erosion surface is healed, and acetic acid white epithelium is not seen. [ case 2] female 68 years old first visit time: year 2020, 6 and 30.

The main complaints are: one month after the cervical conization. The medical history: screening HPV type 16 positive in an outpatient service in 2019; biopsy pathology in 1/15/2020: HSIL. Cervical conization in a certain Beijing hospital in 5 months in 2020, postoperative pathology: cervical CINII-III, the affected glands are not clean. Post-operative review of HPV: HR-HPV16 type positive. After the postoperative administration of the honeysuckle liquid and the blue and white flower powder is performed locally, the medicines are applied for 3 months, and then the investigation is repeated after the medicines are stopped for one month: the wound surface is well healed after the cervical conization, HPV16 type is negative, and the HSIL is used for reducing LSIL accompanied chronic inflammation and the vaginal wall VAINI is obtained by taking multipoint biopsy of the cervix under a colposcope. Continuously administering the honeysuckle liquid and the blue and white flower powder locally for 3 months.

Case 3] female 57 years old, date of first visit: year 2020, month 7 and day 10.

The main complaints are: cervical HR-HPV infection was found for two years. The medical history: the patient is the uterine sarcoma, and the patient is subjected to full uterine curettage, double attachments and pelvic sweep for 2 years. Postoperative pathology shows: uterine sarcoma, cervical HSIL, with unclean margins. And (4) reviewing HPV: positive for HR-HPV16 type, TCT: inflammatory reactivity changes. Gynecological examination: congestion of vulva and vaginal mucosa, less secretion amount, congestion of vaginal wall and cervix, atrophy, blood touch (+), empty pelvic cavity and no obvious abnormality.

Treatment: 1. the traditional Chinese medicine composition can be used for diminishing inflammation and treating the senile vaginitis after artificial menopause by applying the traditional Chinese medicine composition to the vagina, the local inflammation is relieved after the traditional Chinese medicine composition is applied to the vagina for 10 days, and the local inflammation at the broken end of the vagina is cured after the traditional Chinese medicine composition is applied to the vagina in a hospital for 3 months. The HPV is rechecked after the medicine is taken for 3 months and is stopped for one month: all were negative. TCT: no obvious abnormality was observed. Review after 6 months is recommended.

Case 4] female 66 years complaint: itching of the external genitals, and half a year of bad smell, the diagnosis comes.

The medical history: menopause is 15 years, pruritus vulvae in the last half year, secretion has peculiar smell, excrement is dried for 2-3 days, and urine is normal. A pale-red tongue with yellow coating, thin and greasy coating and a wiry and slippery pulse. Gynecological examination: the vulva and the vagina are normal, the secretion amount is middle, turbidity is generated, cervical erosion is I degree, the contact blood is positive, and the double attachments of the uterus are not obviously abnormal. Checking TCT: ASC-US; HPV: negative, soreness of the waist and discomfort in the lower abdomen. Cervical biopsy results: cervical 3 points, chronic cervicitis, 1, 6, 9 points LSIL. Examination of vaginal secretion: clue positive cells, cleanliness III.

Local application of the Qinghua powder and the Lian Hua liquid in the vagina of a hospital for 3 months, and rechecking TCT: moderate inflammation is shown; HPV: and (4) negativity. And (3) reviewing gynecological internal diagnosis: the cervical erosion surface heals, the inflammation heals and the surface is smooth.

Case 5] female 35-year-old complaints: physical examination revealed cervical HR-HPV infection for 3 months. The medical history: the menstrual regularity is normal, 1 is produced during pregnancy, ovarian cyst is found one year ago, and cervical HR-HPV infection is found by physical examination. Auxiliary inspection: cervical TCT: moderate inflammation; HPV: high risk type 66 positivity; mycoplasma: UU (+) secretion conventional: BV (+), cleanliness IV; pelvic color ultrasonography: left ovarian cyst: 5.2 x 3.3cm for chocolate cysts, 7.6 x 5.0cm for hydrotubation. CA 125: 26.9U/ml, CA 199: 49.48U/ml; HE 4: 52.4. mu.g/L.

Gynecological examination: the vulva and the vagina are normal, the secretion amount is medium, the quality is thin, the cervix is large, the erosion degree is III, the bleeding is positive, the anteposition of the uterus is normal in size and hard in texture, the activity is poor, the left accessory is thickened, and the mass of the goose egg is large, so that no obvious tenderness exists; the right appendix was not as clearly abnormal. Treatment: the Qinghua powder and the Lian Hua liquid are applied for 3 months, the oral blood stasis removing and toxin expelling uterus protecting soup is taken from 1 day of menstruation to clean menstruation to improve the immunity and control pelvic cavity masses, and the inspection is carried out after the medicine is stopped for one month. Cervical TCT: mild inflammation; HPV: and (4) negativity. The cervical erosion surface turns to I degree. CA 125: 25.9U/ml; CA 199: 42.48U/ml.

Case 6] women 51 initial diagnosis date 2020.9.25 chief complaints: the symptomatology is painful for one month.

The medical history: the patient had menstrual arrest for half a year and was closing the house for nearly one month with pain. Normal stool and diet, pale-red tongue with thin and greasy coating. Gynecological examination: the vulva and the vagina are normal, the surface of the cervix is smooth, the uterine neck is positive in blood contact, and the double attachments of the uterus are not abnormal. Examination of cervical TCT for moderate inflammation, HPV: type 16 positive. Colposcope, the surface of cervix is smooth, no obvious acetic acid white epithelium is seen, and iodine is not colored. Taking cervical biopsy results show that: cervical 3.6.9 point LSIL; HSIL (CINII) is considered at point 12 and in the cervical canal. Cervical 12-point immunohistochemistry hsil (cinii), P16: partial squamous epithelium full (+), Ki67: about 20% (+). Treatment: the medicine is applied to the cervix for 3 months by using the Qinghua powder and the Lian Hua liquid. TCT mild inflammation, HPV: negative reexamination colposcope takes multipoint cervical biopsy, and the pathology shows: cervical 3, 5-6, 9, 12-1 point LSIL, cervical canal: see mucus-like material and inflammatory cells. The patient continues to treat the Qinghua powder and the Lian Hua liquid for 3 months at present and is rechecked after stopping taking the medicine for half a year.

Case 7 patients were 37 years old female, and were found to be positive for cervical HR-HPV infection, type 18, 56, by routine physical examination prior to HPV vaccination. At the level of II cervical erosion under colposcopy, a thin layer of acetic acid white epithelium is visible. Taking cervical biopsy, and showing pathology: chronic cervicitis with focal HSIL suggested immunohistochemistry. Immunohistochemistry results: chronic cervicitis, metaplasia of the fallopian tubes of a portion of the glands. P16 (-) Ki67 part glandular epithelial cell 40% (+), Vitentin part (+) ER (+), PR (+)

The patient refuses physical treatment and Chinese medicine is conservative treatment. Treatment: if the Qinghua powder and the Lian Hua liquid are applied for 1 month, the patient can not be applied in hospital because of going out, so that the HPV56 type positive and 18 type negative are rechecked after the patient stops applying the medicine for one month by himself.

It should be understood that the detailed description of the present invention is only for illustrating the present invention and is not limited by the technical solutions described in the embodiments of the present invention, and those skilled in the art should understand that the present invention can be modified or substituted equally to achieve the same technical effects; as long as the use requirements are met, the method is within the protection scope of the invention.

Claims

1. A combined prescription for treating cervical HPV infection is characterized by comprising honeysuckle liquid and blue-and-white flower powder in parts by weight;

2. The combination formulation for the treatment of cervical HPV infection according to claim 1, wherein: the honeysuckle flower liquid comprises 3g of coptis chinensis, 10g of honeysuckle, 10g of curcuma zedoary, 5g of fructus cnidii, 5g of rhizoma bletillae, 3g of brucea javanica, 10g of folium isatidis, 3g of catechu, 5g of dried alum, 5g of radix sophorae flavescentis, 10g of frankincense, 5g of gallnut, 5g of raw astragalus membranaceus and 3g of liquorice.

3. The combination formulation for the treatment of cervical HPV infection according to claim 2, wherein: the honeysuckle flower powder comprises 5g of indigo naturalis, 10g of honeysuckle, 10g of coptis chinensis, 1g of dragon's blood, 5g of rhizoma bletillae, 10g of catechu, 2g of dried alum and 3g of liquorice.

4. The combination formulation for the treatment of cervical HPV infection according to claim 1, wherein: the honeysuckle liquid comprises 15g of coptis chinensis, 40g of honeysuckle, 25g of curcuma zedoary, 30g of fructus cnidii, 45g of rhizoma bletillae, 45g of brucea javanica, 50g of folium isatidis, 25g of catechu, 25g of dried alum, 40g of radix sophorae flavescentis, 50g of frankincense, 45g of gallnut, 50g of raw astragalus membranaceus and 25g of liquorice.

5. The combination formulation for the treatment of cervical HPV infection according to claim 4, wherein: the honeysuckle flower powder comprises 100g of indigo naturalis, 70g of honeysuckle, 75g of coptis chinensis, 75g of dragon's blood, 70g of rhizoma bletillae, 80g of catechu, 75g of dried alum and 25g of liquorice.

6. The combination formulation for the treatment of cervical HPV infection according to claim 1, wherein: the honeysuckle liquid comprises 50g of coptis chinensis, 100g of honeysuckle, 50g of curcuma zedoary, 60g of fructus cnidii, 90g of rhizoma bletillae, 90g of brucea javanica, 60g of folium isatidis, 40g of catechu, 50g of dried alum, 60g of radix sophorae flavescentis, 100g of frankincense, 90g of gallnut, 100g of raw astragalus mongholicus and 30g of liquorice.

7. The combination formulation for the treatment of cervical HPV infection according to claim 6, wherein: the honeysuckle flower powder comprises 200g of indigo naturalis, 150g of honeysuckle, 150g of coptis chinensis, 100g of dragon's blood, 150g of rhizoma bletillae, 150g of catechu, 150g of dried alum and 50g of liquorice.