Disclosure of Invention
In order to solve the technical problems, the invention provides a traditional Chinese medicine composition for preventing and treating gout diseases of birds.
The traditional Chinese medicine composition contains active ingredients, wherein the active ingredients are prepared from rhizoma atractylodis, caulis sinomenii, radix clematidis, cortex phellodendri, rhizoma coptidis, polygonum cuspidatum, edible tulip, rhizoma dioscoreae septemlobae, rhizoma smilacis glabrae, cortex magnoliae officinalis, liquorice, herba mascot, camphor tree seeds, vitamin A, potassium citrate and sulfuric acid.
Further, the active ingredients are prepared from, by weight, 10-40 parts of rhizoma atractylodis, 5-30 parts of caulis sinomenii, 6-18 parts of radix clematidis, 5-30 parts of cortex phellodendri, 2-10 parts of rhizoma coptidis, 20-50 parts of polygonum cuspidatum, 5-25 parts of edible tulip, 5-20 parts of rhizoma dioscoreae, 5-25 parts of rhizoma smilacis glabrae, 5-30 parts of magnolia officinalis, 2-10 parts of liquorice, 20-50 parts of mascot, 20-30 parts of camphor tree seed, 1-9 parts of vitamin A, 3-12 parts of potassium citrate and 1-5 parts of taurine.
Further, the active ingredients are prepared from 15-30 parts by weight of rhizoma atractylodis, 10-25 parts by weight of caulis sinomenii, 10-16 parts by weight of radix clematidis, 10-20 parts by weight of cortex phellodendri, 4-8 parts by weight of rhizoma coptidis, 20-40 parts by weight of polygonum cuspidatum, 10-20 parts by weight of edible tulip, 10-16 parts by weight of rhizoma dioscoreae, 8-22 parts by weight of rhizoma smilacis glabrae, 10-24 parts by weight of cortex magnoliae officinalis, 4-8 parts by weight of liquorice, 30-45 parts by weight of mascot, 24-28 parts by weight of camphor tree seed, 3-6 parts by weight of vitamin A, 5-10 parts by weight of potassium citrate and 2-4 parts by weight of taurine.
Further, the active ingredients are prepared from 22 parts by weight of rhizoma atractylodis, 20 parts by weight of caulis sinomenii, 14 parts by weight of radix clematidis, 12 parts by weight of cortex phellodendri, 6 parts by weight of rhizoma coptidis, 30 parts by weight of polygonum cuspidatum, 18 parts by weight of edible tulip, 12 parts by weight of rhizoma dioscoreae, 18 parts by weight of rhizoma smilacis glabrae, 20 parts by weight of magnolia officinalis, 6 parts by weight of liquorice, 38 parts by weight of mascot, 26 parts by weight of camphor tree seed, 5 parts by weight of vitamin A, 7 parts by weight of potassium citrate and 3 parts by weight of taurine.
The invention also aims to provide a preparation method of a traditional Chinese medicine composition for preventing and treating gout of birds, which comprises the following steps:
(1) Weighing the raw materials of the medicines according to the weight ratio;
(2) Coarsely crushing the traditional Chinese medicine raw materials obtained in the step (1), soaking the raw materials in distilled water for 0.5h, decocting for 3 times, wherein the first time is 2 hours, the second time and the third time are respectively 1.0h, mixing the filtrates, and concentrating into thick paste for later use.
(3) Mixing the vitamin A and taurine weighed in the step (1) with proper amount of starch and dextrin uniformly, and then mixing with the thick paste in the step (2) uniformly to prepare the finished product.
The traditional Chinese medicine composition for preventing and treating the gout of the poultry, which is prepared by the preparation method, can be prepared into the following dosage forms: soluble powder, granule or oral liquid.
Furthermore, the traditional Chinese medicine composition powder for preventing and treating the gout disease of the poultry, which is prepared by the preparation method, has the medicine particle size below 70 microns.
The invention also provides application of the traditional Chinese medicine composition in preventing and treating gout diseases of birds.
In particular to a medicine for preventing and treating gout of birds by preparing the traditional Chinese medicine composition.
Furthermore, the invention also provides a poultry feed containing the traditional Chinese medicine composition.
The medicine has the advantages and beneficial effects that:
the traditional Chinese medicine composition is prepared by repeated verification and practice through a large number of clinical tests according to dialectical theory of Chinese veterinary prescriptions. The prescription of the invention combines pathogenesis and clinical symptoms of the gout disease of the poultry, and the treatment principle is an accurate prescription obtained by a great amount of clinical effect verification under the guidance of the diagnosis and treatment theory of the traditional Chinese medicine, thereby having good prevention and treatment effects on the gout disease of the poultry.
The fowl gout belongs to the categories of 'joint disease', 'arthralgia syndrome' in traditional Chinese medicine, the wind damp-heat arthralgia syndrome, the damp evil qi is remained without going, the heat is accumulated for a long time, and the fowl gout is infused into joints to cause qi and blood occlusion, blood vessels are not smooth, and the onset of the fowl gout is 5 important pathological links of the gout, namely heat, dampness, phlegm, deficiency and blood stasis. The traditional Chinese medicine diagnosis is that spleen deficiency and damp heat stasis are the principal of disease, spleen dysfunction and transportation are the principal of disease, damp heat phlegm stasis is the principal of disease, eating is favored by fat, sweet and greasy, long-term damp turbidity is generated internally, spleen dysfunction and transportation, invasion of exogenous evil, internal and external diseases are combined, damp heat stasis evil is blocked in tendons and bones, joint swelling and pain are seen, and liver and kidney are damaged after long-term blockage, and symptoms such as edema, hypodynamia and the like are presented. Traditional Chinese medicines for treating gout are selected from ancient times and mainly focused on the medicines for clearing heat and activating blood, strengthening spleen and purging turbid urine, nourishing liver and kidney, promoting diuresis and removing dampness and warming interior. The basic method for treating gout in traditional Chinese medicine is as follows: in the acute attack stage, the treatment is mainly to clear away heat and toxic materials, remove turbid urine, promote diuresis, remove arthralgia and dredge collaterals; in the rest period, the treatment mainly aims at strengthening spleen, eliminating dampness, tonifying liver and kidney, relaxing menstruation, dredging arthralgia and dredging collaterals. The occurrence of gout in birds is related to disharmony of viscera, disturbance of essence and endowment, and the like, and damp turbidity and toxin are pathogenic factors. The poultry gout belongs to bladder damp-heat and liver-gallbladder stagnation on middle veterinarian syndrome type classification, and is similar to sand and stone stranguria on middle veterinarian, and the principle of traditional Chinese medicine treatment is mainly to promote diuresis and remove dampness, clear heat, invigorate spleen and the like. The traditional Chinese medicine for promoting diuresis and removing dampness is suitable for treating stranguria and turbid urine (urinary system infection or calculus) and other symptoms besides edema, and general pharmacological actions include promoting urination and resisting bacteria, promoting bile flow and protecting liver, regulating immunity and the like, and can promote excretion of potassium, sodium, chlorine and other ions while promoting excretion of water. The traditional Chinese medicine has unique advantages in treating gout, which are different from western medicines, and the action mechanism is mainly focused on reducing uric acid level, inhibiting inflammatory reaction, resisting oxidative stress, regulating signal paths and the like.
In the patent of the invention, the giant knotweed and the rhizoma atractylodis are used as monarch drugs, wherein the giant knotweed has the effects of promoting diuresis and removing jaundice, clearing heat and detoxicating, removing blood stasis and relieving pain, and the resveratrol in the giant knotweed can inhibit the activity of xanthine oxidase and inhibit uric acid transport protein of kidney to doubly regulate and reduce the concentration of serum uric acid; rhizoma Atractylodis can dispel dampness and invigorate spleen, and has effects of removing pathogenic dampness, relieving exterior syndrome, dispelling pathogenic wind-dampness, and drying spleen and stomach dampness. Caulis Sinomenii, radix Clematidis, herba Reineckeae Carneae, cortex Phellodendri, and Coptidis rhizoma as ministerial drugs, wherein caulis Sinomenii has effects of dispelling pathogenic wind and dampness, dredging meridian, and relieving pain due to wind-dampness, joint swelling, and paralysis and itching, and has neutralizing effect on uric acid. The Chinese clematis root has the effects of dispelling wind and removing dampness, and activating collaterals and relieving pain, is mainly used for treating the symptoms of rheumatalgia, limb numbness, difficulty in flexing and extending, spasm of tendons and vessels and the like, and has the main effects of removing dampness, activating collaterals and relieving pain, and aims at the pathogenesis characteristics of pain, such as Yu Lao fatigue and pain caused by obstruction. The Reineckea carnea has the effects of moistening lung for arresting cough, reinforcing kidney and setting bone, and has the main effects of reinforcing kidney and setting bone in the invention patent. The berberine which is the main component in the coptis chinensis and the phellodendron barks deprives the catalytic action of xanthine oxidase, thereby playing a role in inhibiting the activity of xanthine oxidase and further reducing uric acid deposition. The edible tulip, the yam rhizome, the glabrous greenbrier rhizome, the officinal magnolia bark and the camphor tree are taken as adjuvant drugs, wherein the edible tulip has the functions of clearing heat and detoxicating, eliminating carbuncles and resolving hard mass, and is mainly used for treating furuncle, attacking toxin and breaking skin and resolving various toxins. Dioscorea opposite to the traditional Chinese medicine for treating the kidney function recovery can remove dampness, remove turbidity, expel wind and remove arthralgia, and dioscin in the yam opposite to the traditional Chinese medicine can effectively remove urate deposition and promote kidney function recovery. Rhizoma smilacis glabrae has the effects of promoting joint circulation, strengthening spleen and stomach, strengthening tendons and bones, removing rheumatism, promoting joint circulation and promoting water passage, is an essential drug for treating damp-heat stranguria with turbid urine, carbuncle swelling and plum sores, has an inhibiting effect on inflammatory reaction of urates, and can up regulate the expression of OAT1, organic cation transporter 2 (OCTN 2) and mRNA thereof in kidney tissues by inhibiting the activity of xanthine oxidase, so that urate deposition is obviously reduced. The magnolia officinalis has the effects of resolving dampness, removing stagnation, promoting qi circulation, relieving asthma, resolving food stagnation, dispelling pathogenic wind, relieving pain, and the effective components of phenols and alkalis of magnolia officinalis can promote the discharge of uric acid salts. Camphor fruit, fructus Laurae, with effects of dispelling cold and dampness, promoting qi circulation and relieving pain, and treating stomach cold and abdominal pain and swelling toxin. The invention has the main effects of dispelling wind and cold, eliminating dampness and detumescence. Glycyrrhrizae radix is used as guiding drug for clearing heat and detoxicating, eliminating phlegm and relieving cough. Can be used for treating stomach ache, abdominal pain, and gastrocnemius spasm pain.
Vitamin A can avoid uric acid excretion disorder caused by atrophy, keratinization and abscission of renal epithelial cells. Potassium citrate regulates water salt metabolism and acid-base balance in poultry. Taurine participates in regulating cell volume, provides a foundation for bile salt formation, plays an important role in the modulation of intracellular free calcium concentration, and plays an important role in protecting renal dysfunction and normal development. Taurine has the functions of diminishing inflammation, easing pain, maintaining the osmotic balance of the organism, regulating the digestion and absorption of lipid, increasing the solubility of lipid and cholesterol, relieving bile obstruction, reducing the cytotoxicity of certain free bile acid, inhibiting the formation of cholesterol stones, and increasing the bile flow and has the function of enhancing immunity. The medicines are combined to play roles of clearing heat and activating blood, nourishing liver and kidney, strengthening spleen and purging turbid urine, promoting diuresis and removing dampness, relieving swelling and treating stranguria, inducing resuscitation and removing stasis, purging pathogenic fire and expelling toxin, achieving the effects of inducing diuresis and relieving swelling and strengthening metabolism, and can effectively promote the excretion of the uric acid salt, avoid the damage of the deposition of the uric acid salt to organisms and have remarkable treatment effect on the poultry gout.
Compared with the prior art, the medicine has obvious effect of preventing and treating the gout of the poultry. The invention can further reduce the serum uric acid content of animals by adding vitamins, potassium citrate and taurine into the formula.
Detailed Description
The following examples are illustrative of the invention and are not intended to limit the scope of the invention.
EXAMPLE 1 preparation and use of the medicament of the invention
(1) Weighing the following raw materials by weight:
10 parts of rhizoma atractylodis, 5 parts of caulis sinomenii, 6 parts of radix clematidis, 5 parts of cortex phellodendri, 2 parts of rhizoma coptidis, 20 parts of polygonum cuspidatum, 5 parts of edible tulip, 5 parts of rhizoma dioscoreae, 5 parts of rhizoma smilacis glabrae, 5 parts of magnolia officinalis, 2 parts of liquorice, 20 parts of mascot, 20 parts of camphor tree seed, 1 part of vitamin A, 3 parts of potassium citrate and 1 part of taurine.
(2) Coarse pulverizing the raw materials of the traditional Chinese medicines with the weight, which are weighed in the step (1), soaking the raw materials in distilled water for 0.5h, decocting for 3 times, wherein the first time is 2 hours, the second time and the third time are respectively 1.0h, mixing the filtrates, and concentrating into thick paste for later use.
(3) Mixing vitamin A and taurine weighed in the step (1) with proper amount of starch and dextrin uniformly, and then mixing with the thick paste in the step (2) uniformly to prepare granules.
(4) The application method of the medicine comprises the following steps: the mixed drink is used for preventing and treating the gout of the poultry: 0.5kg of the traditional Chinese medicine is added into each 1 ton of water, and the water is drunk freely for 7 to 10 days.
EXAMPLE 2 preparation and use of the medicament of the invention
(1) Weighing the following raw materials by weight:
40 parts of rhizoma atractylodis, 30 parts of caulis sinomenii, 18 parts of radix clematidis, 30 parts of cortex phellodendri, 10 parts of rhizoma coptidis, 50 parts of polygonum cuspidatum, 25 parts of edible tulip, 20 parts of rhizoma dioscoreae, 25 parts of rhizoma smilacis glabrae, 30 parts of magnolia officinalis, 10 parts of liquorice, 50 parts of mascot, 30 parts of camphor tree, 9 parts of vitamin A, 12 parts of potassium citrate and 5 parts of taurine. Prepared and used as in example 1.
EXAMPLE 3 preparation and use of the medicament of the invention
(1) Weighing the following raw materials by weight:
15 parts of rhizoma atractylodis, 10 parts of caulis sinomenii, 10 parts of radix clematidis, 10 parts of cortex phellodendri, 4 parts of rhizoma coptidis, 20 parts of polygonum cuspidatum, 10 parts of edible tulip, 10 parts of rhizoma dioscoreae, 8 parts of rhizoma smilacis glabrae, 10 parts of magnolia officinalis, 4 parts of liquorice, 30 parts of mascot, 24 parts of camphor tree, 3 parts of vitamin A, 5 parts of potassium citrate and 2 parts of taurine. Prepared and used as in example 1.
EXAMPLE 4 preparation and use of the medicament of the invention
(1) Weighing the following raw materials by weight:
30 parts of rhizoma atractylodis, 25 parts of caulis sinomenii, 16 parts of radix clematidis, 20 parts of cortex phellodendri, 8 parts of rhizoma coptidis, 40 parts of polygonum cuspidatum, 20 parts of edible tulip, 16 parts of rhizoma dioscoreae, 22 parts of rhizoma smilacis glabrae, 24 parts of magnolia officinalis, 8 parts of liquorice, 45 parts of mascot, 28 parts of camphor tree, 6 parts of vitamin A, 10 parts of potassium citrate and 4 parts of taurine. Prepared and used as in example 1.
EXAMPLE 5 preparation and use of the medicament of the invention
(1) Weighing the following raw materials by weight:
22 parts of rhizoma atractylodis, 20 parts of caulis sinomenii, 14 parts of radix clematidis, 12 parts of cortex phellodendri, 6 parts of rhizoma coptidis, 30 parts of polygonum cuspidatum, 18 parts of edible tulip, 12 parts of rhizoma dioscoreae, 18 parts of rhizoma smilacis glabrae, 20 parts of magnolia officinalis, 6 parts of liquorice, 38 parts of mascot, 26 parts of camphor tree, 5 parts of vitamin A, 7 parts of potassium citrate and 3 parts of taurine. Prepared and used as in example 1.
Comparative example 1
A traditional Chinese medicine composition for preventing and treating gout of birds comprises the following components in parts by weight: 2 parts of glabrous greenbrier rhizome, 2 parts of pagodatree flower, 2 parts of climbing fern spore, 1.5 parts of akebia stem, 1 part of coptis chinensis, 2 parts of liquorice, 1 part of astragalus mongholicus, 1 part of hawthorn and 1 part of medicated leaven.
The preparation method of the traditional Chinese medicine composition for preventing and treating the gout of the poultry comprises the following steps:
weighing the traditional Chinese medicine according to the proportion, coarsely crushing, extracting for 2 times by a water extraction method, extracting for 1.5 hours at 75 ℃ for 20 times of water for the first time, extracting for 1.0 hour at 75 ℃ for 10 times of water for the second time, combining the two extracting solutions, filtering, and concentrating the filtrate under reduced pressure and vacuum to 1g/mL to obtain the traditional Chinese medicine.
Comparative example 2
A traditional Chinese veterinary medicine for treating gout comprises the following components in parts by weight: 7 parts of catechu, 7 parts of frankincense, 12 parts of ash bark, 12 parts of amur corktree bark and 12 parts of swordlike atractylodes rhizome.
The preparation method of the traditional Chinese veterinary medicine for treating gout comprises the following steps: weighing the traditional Chinese medicines in parts by weight, coarsely crushing, adding 6 times of 60% ethanol for extraction for 3 times, each time for 1h, combining the extracting solutions, filtering, concentrating the filtrate under reduced pressure to obtain a relative density of 1.05, adding a proper amount of auxiliary materials, and preparing into granules.
Comparative example 3
The medicine for treating acute gouty arthritis comprises the following components in parts by weight: 20 parts of honeysuckle, 20 parts of sargentgloryvine stem, 12 parts of Indian iphigenia bulb, 20 parts of clematis root, 20 parts of coix seed, 20 parts of medicinal cyathula root, 30 parts of lysimachia christinae hance, 20 parts of yam rhizome, 30 parts of glabrous greenbrier rhizome, 20 parts of ash bark, 20 parts of grifola frondosa, 20 parts of caulis sinomenii, 15 parts of stephania tetrandrae, 20 parts of alisma orientale, 10 parts of fructus Litseae, 9 parts of rhizoma pinelliae preparata, 20 parts of plantain herb, 9 parts of safflower, 9 parts of costustoot, 10 parts of cassia seed, 6 parts of long pepper, 15 parts of plantain seed, 10 parts of amur corktree bark, 12 parts of rhizoma corydalis, 12 parts of red paeony root, 12 parts of giant knotweed rhizome, 15 parts of swordlike atractylodes rhizome, 10 parts of clematis root, 12 parts of Chinese taxillus twig, 6 parts of rhubarb, 12 parts of tree peony root, 12 parts of root of pubescent angelica and 6 parts of liquorice.
The preparation method of the medicine for treating acute gouty arthritis comprises the following steps: weighing the traditional Chinese medicines in parts by weight, extracting with 10 times of water twice for 2 hours each time, mixing the extracting solutions, filtering, and concentrating the filtrate under reduced pressure until the relative density is 1.2.
Comparative example 4
The traditional Chinese medicine for treating gouty arthritis comprises the following components in parts by weight: 10 parts of amur corktree bark, 10 parts of swordlike atractylodes rhizome, 30 parts of glabrous greenbrier rhizome, 10 parts of liquoric root, 15 parts of Chinese clematis root, 10 parts of red paeony root, 15 parts of giant knotweed rhizome, 10 parts of Chinese angelica, 8 parts of Indian iphigenia bulb, 10 parts of pubescent angelica root, 15 parts of pilose asiabell root and 10 parts of wine rhubarb.
The preparation method of the traditional Chinese medicine for treating gouty arthritis comprises the following steps: weighing the traditional Chinese medicines in parts by weight, extracting with 10 times of water twice for 2 hours each time, mixing the extracting solutions, filtering, and concentrating the filtrate under reduced pressure until the relative density is 1.2.
Comparative example 5
The composition comprises the following components in parts by weight: 22 parts of rhizoma atractylodis, 20 parts of caulis sinomenii, 14 parts of radix clematidis, 12 parts of cortex phellodendri, 6 parts of coptis chinensis, 30 parts of polygonum cuspidatum, 18 parts of edible tulip, 12 parts of rhizoma dioscoreae, 18 parts of rhizoma smilacis glabrae, 20 parts of magnolia officinalis, 6 parts of liquorice, 38 parts of mascot, and 26 parts of camphor tree.
The preparation method of comparative example 5 is:
(1) Weighing the following raw materials by weight:
10 parts of rhizoma atractylodis, 5 parts of caulis sinomenii, 6 parts of radix clematidis, 5 parts of cortex phellodendri, 2 parts of rhizoma coptidis, 20 parts of polygonum cuspidatum, 5 parts of edible tulip, 5 parts of rhizoma dioscoreae, 5 parts of rhizoma smilacis glabrae, 5 parts of magnolia officinalis, 2 parts of liquorice, 20 parts of mascot, and 20 parts of camphor tree.
(2) Coarse pulverizing the raw materials of the traditional Chinese medicines with the weight, which are weighed in the step (1), soaking the raw materials in distilled water for 0.5h, decocting for 3 times, wherein the first time is 2 hours, the second time and the third time are respectively 1.0h, mixing the filtrates, and concentrating into thick paste for later use.
(3) Adding proper amount of starch and dextrin into the thick paste in the step 2, mixing and preparing into particles.
In order to show the prevention and treatment effect of the medicine on the gout disease of the poultry, the clinical curative effect observation test is carried out on the medicine, and the beneficial effects of the medicine are further illustrated by test examples.
Test example 1
In order to examine the effect of the drug of the invention on uric acid reduction, the test of potassium oxazinate to hyperuricemia in mice was adopted in animal laboratories of the national institute of motion protection in Dabei in 2020.
1 test drug and apparatus
1.1 test article: the medicines prepared in examples 1-5, the medicines prepared in comparative examples 1-5, allopurinol, gout-relieving capsules, physiological saline, potassium oxazinate, a mouse stomach-lavage needle, a syringe, a blood collection tube and a centrifuge.
1.2 test animals: kunming mice
2 test method
The new Kunming mice with the weight of 16-18g were selected and randomly divided into 14 test groups after 2 days of normal feeding, 10 test groups, test 1 group-test 5 group are respectively the drug of the invention example 1 to example 5, test 6 group-test 10 group are respectively the comparative example 1 to comparative example 5, test 11 ancestor is gout peace capsule group, test 12 group is allopurinol group (positive drug group), test 13 group is model group, and test 14 group is normal group. The same volume of physiological saline was infused in the test 13 group and the test 14 group, and the drugs prepared in examples 1 to 5 of the drugs according to the present invention were administered in the test 1 group to the test 5 group, respectively, and the drugs prepared in examples 1 to 5 were administered in 1mL of a solution corresponding to 1g of the drug according to the present invention, and in 20g/kg, 1 time of gastric administration was administered daily in the morning, followed by 5 days of continuous administration. Test 6 to 10 groups were respectively administered with the drugs prepared in comparative examples 1 to 5, and the drugs prepared in comparative examples 1 to 5 were administered 1mL of a solution corresponding to 1g of the drug of comparative example, 1 time a day at the morning by gavage, and 5 days continuously. The gouning control drug was administered to the trial 11 group at a dose of 20g/kg, 1 time per day by gavage in the morning for 5 consecutive days. The control drug allopurinol was administered to the test group 12 at a dose of 0.02g/kg 1 time per day by gavage in the morning for 5 consecutive days. The last day of administration 1 hour before, test 1 group to test 13 group with potassium oxazinate 650mg/kg intraperitoneal injection molding, test 14 group injection 0.6% sodium carboxymethyl cellulose, 1.5mL after 1 hour, orbital vein blood collection, room temperature standing for 1 hour, 4000rmp centrifugation for 10min, supernatant, phosphotungstic acid reduction method detection serum uric acid level.
Principle of detecting serum uric acid level by phosphotungstic acid method reduction method: uric acid in deproteinized blood filtrate is oxidized into allantoin and carbon dioxide by tungstic acid in alkaline solution, phosphotungstic acid is reduced into tungsten blue in the reaction, the production amount of tungsten blue is in direct proportion to the uric acid content in reaction liquid, and colorimetric determination can be carried out.
Test reagent and test for detecting serum uric acid level by phosphotungstic acid method reduction method
3 operation steps
3.1 test reagents
3.1.1 phosphotungstic acid stock solution: 50g of sodium tungstate was weighed, dissolved in about 400ml of distilled water, 40ml of concentrated phosphoric acid and glass particles were added, boiled water was returned to cool to room temperature, diluted to 1 liter with distilled water, and stored in a brown reagent bottle.
3.1.2 phosphotungstic acid application liquid: 10ml of the phosphotungstic acid stock solution was taken and diluted to 100ml with distilled water.
3.1.3 0.3mol/L sodium tungstate solution: 100g of sodium tungstate (Na 2WO42H2O, MW 329.36) was weighed out, dissolved in distilled water and diluted to 1 liter.
3.1.4 0.33mol/L sulfuric acid: 18.5ml of concentrated sulfuric acid was taken and added to 500ml of distilled water, followed by dilution with distilled water to 1 liter.
3.1.5 tungstic acid reagent: 50ml of a 0.3mol/L sodium tungstate solution, 0.05ml of concentrated phosphoric acid and 50ml of 0.33mol/L sulfuric acid were added to 800ml of distilled water, and the mixture was mixed well.
3.1.6 lmol/L sodium carbonate solution: 10.6g of anhydrous sodium phosphate solution was weighed in distilled water, diluted to 1 liter and placed in a plastic flask, and if cloudy, filtered for use.
3.1.7 6.0mol/L uric acid standard stock solution: 60mg of lithium carbonate (AR) is dissolved in 40ml of distilled water and heated to 60 ℃ to be completely dissolved, 100.9mg of uric acid (MW 168.11) is accurately weighed and dissolved in the lithium carbonate solution, the solution is cooled and placed at room temperature, transferred into a 1000ml volumetric flask, diluted to a scale with distilled water and stored in a brown flask.
3.2 test operation method
Taking 16 test tubes with the scale of 16mm multiplied by 00mm, adding 4.5ml of tungstic acid reagent into each test tube, respectively adding 0.5ml of serum of test 1-14 into test tubes with the scale of 1-116 and the scale of 1-14, adding 0.5ml of standard application liquid into test tubes with the scale of 15, adding 0.5ml of distilled water into test tubes with the scale of 16, uniformly mixing, standing for 10min, centrifugally precipitating the test tubes with the scale of 1-14, respectively taking 2.5ml of supernatant liquid with the scale of 1-16, respectively adding 0.5ml of sodium carbonate solution, uniformly mixing, standing for 10min, adding 0.5ml of phosphotungstic acid application liquid, uniformly mixing, standing for 20min, zeroing the solution of test tubes with the scale of 16 with a blank tube at the wavelength of 660nm by using a spectrophotometer, and reading the absorbance of each tube. The procedure is as in Table 1
TABLE 1 operation of phosphotungstic acid reduction method for detecting serum uric acid
Serum uric acid calculation: serum uric acid μmol/l=assay tube absorbance/standard tube absorbance×300
4 test results and conclusions
TABLE 2 Effect of the inventive drug on hyperuricemia mice serum uric acid caused by Potassium Oxonate
As can be seen from Table 2, compared with the model control group, the medicine of the invention, the comparative example medicine and the positive control medicine, and the medicine for treating gout, fengshuning capsule, which is commonly used in the market, can remarkably reduce serum uric acid of mice. The effect of the medicine for reducing serum uric acid is stronger than that of the medicine for treating gout, namely allopurinol, which is a comparative example medicine and a positive control medicine. Compared with the test group 10, the test group 5 has better uric acid reducing effect on the serum of mice, that is, the medicine of the example 5 has better uric acid reducing effect on the serum of mice than the medicine of the comparative example 5, which shows that three substances of vitamin A, potassium citrate and sulfuric acid have synergistic effect on the uric acid reducing effect on the serum of mice of the traditional Chinese medicine composition. The test 5 groups of the medicine have the best uric acid reducing effect on the serum of mice, and are the optimal prescription of the invention.
Test example 2
In 8 months 2019, 50000 laying hen houses raised by Jiangsu Wang Mou are characterized by sudden enlargement of joints of action legs, slow action, lameness, anorexia, diarrhea and the like, and the veterinary clinic diagnosis shows that the chickens are gout diseases, and the medicines prepared in the example 5 are used for treating and dosing a plurality of chicken houses with the disease, and the medicines sold in the market for treating gout are used for treating for 10 days. After half a month, the service staff return visit, and more than 90% of chickens in the chicken house treated by the medicament prepared in the example 5 are recovered to be normal, and the mental state is good. And 60% of chickens in another henhouse treated by the medicines sold in the market for treating gout are recovered to be normal, and some chickens are accompanied with red and swollen joints. The medicine feedback of Wang Mou shows that the medicine has good treatment effect on chicken gout.
The foregoing is merely a preferred embodiment of the present invention, and it should be noted that it will be apparent to those skilled in the art that several modifications and variations can be made without departing from the technical principle of the present invention, and these modifications and variations should also be regarded as the scope of the invention.