CN113476355B

CN113476355B - Plant extraction compound for relieving itching and diminishing inflammation as well as preparation method and application thereof

Info

Publication number: CN113476355B
Application number: CN202110420972.6A
Authority: CN
Inventors: 罗伟; 刘学生; 曹文豪
Original assignee: Hunan Xianwei Sunshine Biotechnology Co ltd
Current assignee: Hunan Xianwei Sunshine Biotechnology Co ltd
Priority date: 2021-04-19
Filing date: 2021-04-19
Publication date: 2022-09-13
Anticipated expiration: 2041-04-19
Also published as: CN113476355A

Abstract

The invention relates to the field of plant extracts, in particular to a plant extract compound for relieving itching and diminishing inflammation and a preparation method and application thereof. The plant extract compound is prepared from rosemary extract, galangal extract and gallnut extract. The rosemary extract and the galangal extract obtained by supercritical carbon dioxide extraction are used for avoiding flushing or erythema caused by inflammatory medium or lipid medium, have high effects of fading red, removing speckles and relieving itching, are beneficial to inhibiting burning and stabbing pain, and have high safety. Adding Galla chinensis extract to promote clear and transparent solution, and keeping stability at high and low temperature. The plant extraction compound provided by the invention has excellent effects of resisting allergy, relieving itching and diminishing inflammation, is natural, free of side effect, mild and moist, and beneficial to reservation of active ingredients of the raw materials, controls the dosage of the raw materials and achieves the optimal effect superposition.

Description

Plant extraction compound for relieving itching and diminishing inflammation as well as preparation method and application thereof

Technical Field

The invention relates to the field of plant extracts, in particular to a plant extract compound for relieving itching and diminishing inflammation and a preparation method and application thereof.

Background

On the' twenty fourth national skin pathology annual meeting sponsored by the Chinese medical society in 2018, a survey data for the health condition of the skin of Chinese people shows that 45% of patients in China are troubled by the skin sensitivity problem, and the number of people with sensitive muscles in China is always in a high-speed increasing trend from 2018 in 2014. 40% -56% of Asian women are sensitive muscles, about 36% of Chinese women have sensitive muscle troubles, the number of girls affected by the sensitive muscles is about 3 times that of men, and women aged 20-30 are the main group of the sensitive muscles. Dermatological definition of skin allergy: is the state of physiological hyperresponsiveness of the skin to environmental or internal factors (Italy) rather than diseases such as: common skin discomfort symptoms caused by exposure to skin care products, soaps, sunscreen products, detergents, or in dry and cold environments, or diet, overnight, etc., severely affect quality of life, manifested as sensory symptoms: facial tightness, prickling, itching, burning, tingling, pain, persistent itching, etc.; skin surface symptoms: erythema and skin flushing, edema, etc.

Most of the anti-allergic agents in the market are anti-allergic drugs, certain side effects exist on safety, plants are natural and anti-allergic, mild and moist, no side effects exist, safety is high, and the development trend of anti-allergic raw materials in cosmetics is realized. Plants have growth characteristics for adapting to their living environments, for example, various algae living in deep sea have no sunshine, but have strong self-repairing ability and can smoothly propagate, some plants have excellent characteristics of antianaphylaxis, itching relieving, inflammation diminishing and the like, and the plants are taken as antiallergic cosmetic raw materials by fully utilizing plant active ingredients.

However, unstable components exist in the plants, and the problems of instability and decomposition possibly exist in the extraction process, so that the anti-inflammatory and itching-relieving effects are influenced, the components in different plants are complex and various, the effects of different components are difficult to characterize and possibly influence each other, the antioxidant effect is reduced or the extract is unstable, so that the subsequent application in cosmetic products is influenced.

Disclosure of Invention

In order to solve the problems, the invention provides a plant extraction compound for relieving itching and diminishing inflammation, which is prepared from raw materials of plant extracts, wherein the plant extracts comprise 45-65 parts by weight of rosemary extract, 10-15 parts by weight of galangal extract and 3-5 parts by weight of gallnut extract.

As a preferable technical scheme, the rosemary extract comprises rosemary alcohol extract, and the rosemary alcohol extract is prepared by rosemary leaf powder through supercritical carbon dioxide extraction.

As a preferred technical scheme of the invention, the galangal extract is prepared from galangal through supercritical carbon dioxide extraction.

As a preferable technical scheme of the invention, the gallnut extract is prepared by extracting gallnuts with water.

As a preferable technical scheme, the plant extract further comprises 1-5 parts of oat extract by weight.

As a preferred technical scheme of the present invention, the rosemary extract further comprises rosemary leaf water, and the rosemary leaf water is prepared from rosemary leaves through water extraction.

As a preferable technical scheme of the invention, the preparation method of the rosemary leaf water comprises the following steps:

after the rosemary leaves are crushed, steam distillation is carried out for 20-30 min to obtain an oil-water mixture and powder residues, the oil-water mixture is separated to obtain a water phase serving as rosemary leaf water, and the powder residues are dried to serve as rosemary leaf powder.

As a preferred technical solution of the present invention, the plant extract complex further comprises water.

The second aspect of the invention provides a preparation method of the plant extract compound for relieving itching and diminishing inflammation, which comprises the following steps: adding the plant extracts into water, and mixing to obtain the plant extract compound.

The third aspect of the invention provides application of the plant extract compound for relieving itching and diminishing inflammation, which is used for daily chemicals.

Compared with the prior art, the invention has the following beneficial effects:

(1) the invention provides a plant extraction compound, which is prepared by using rosemary extract and galangal extract obtained by supercritical carbon dioxide extraction, avoids flush or erythema caused by inflammatory medium or lipid medium, has high effects of fading, eliminating speckles and relieving itching, is beneficial to inhibiting burning and stinging sensations, and has high safety.

(2) Adding Galla chinensis extract to promote dispersion and dissolution of oil soluble components of herba Rosmarini officinalis extract and rhizoma Alpiniae Officinarum extract obtained by supercritical carbon dioxide extraction in water, and reducing aggregation and particle size of oil soluble components to obtain clear and transparent solution, which is stable at high and low temperature.

(3) The oat extract is added to act together with the rosemary extract, the galangal extract and the gallnut extract, so that the combination of tannic acid and glucoside is inhibited while itching relieving, inflammation diminishing and red fading are promoted, the generation and precipitation of insoluble substances are avoided, a clear and transparent solution is obtained through long-time centrifugation, and the stability of the plant extract in water is improved.

(4) Adding rosemary leaf water and rosemary extract into the plant extract complex together, further promoting the inhibition of the release of histamine and inflammatory factors, controlling and preventing inflammation and anaphylactic reaction. And the water extraction time and the water phase quality of the rosemary leaves are controlled, the loss of active ingredients such as terpenes, flavonoids, rosmarinic acid and the like in the rosemary leaves is reduced, and the retention of the active ingredients is promoted.

(5) The plant extraction compound provided by the invention has excellent effects of resisting allergy, relieving itching and diminishing inflammation, is natural, free of side effect, mild and moist, and beneficial to reservation of active ingredients of the raw materials, controls the dosage of the raw materials and achieves the optimal effect superposition.

Drawings

FIG. 1 shows the results of a test for the degranulation rate of mast cells.

FIG. 2 shows the results of the test of the secretion concentration of inflammatory factors.

Figure 3 shows the results of human skin testing.

Fig. 4 is a photograph of a human skin test, fig. 4 is a photograph after histamine is tried on the left, and fig. 4 is a photograph after histamine is tried on the right, and example 8 is used again.

Detailed Description

The present invention will be more readily understood by reference to the following detailed description of preferred embodiments of the invention and the examples included therein. Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. In case of conflict, the present specification, including definitions, will control.

The term "prepared from …" as used herein is synonymous with "comprising". The terms "comprises," "comprising," "includes," "including," "has," "having," "contains," "containing," or any other variation thereof, as used herein, are intended to cover a non-exclusive inclusion. For example, a composition, process, method, article, or apparatus that comprises a list of elements is not necessarily limited to only those elements but may include other elements not expressly listed or inherent to such composition, process, method, article, or apparatus.

The conjunction "consisting of …" excludes any unspecified elements, steps or components. If used in a claim, the phrase is intended to claim as closed, meaning that it does not contain materials other than those described, except for the conventional impurities associated therewith. When the phrase "consisting of …" appears in a clause of the subject matter of the claims rather than immediately after the subject matter, it defines only the elements described in the clause; other elements are not excluded from the claims as a whole.

When an amount, concentration, or other value or parameter is expressed as a range, preferred range, or as a range of upper preferable values and lower preferable values, this is to be understood as specifically disclosing all ranges formed from any pair of any upper range limit or preferred value and any lower range limit or preferred value, regardless of whether ranges are separately disclosed. For example, when a range of "1 to 5" is disclosed, the described range should be interpreted to include the ranges "1 to 4", "1 to 3", "1 to 2 and 4 to 5", "1 to 3 and 5", and the like. When a range of values is described herein, unless otherwise stated, the range is intended to include the endpoints thereof and all integers and fractions within the range.

The singular forms "a", "an" and "the" include plural referents unless the context clearly dictates otherwise. "optional" or "any" means that the subsequently described event or events may or may not occur, and that the description includes instances where the event occurs and instances where it does not.

Approximating language, as used herein throughout the specification and claims, is intended to modify a quantity, such that the invention is not limited to the specific quantity, but includes portions that are literally received for modification without substantial change in the basic function to which the invention is related. Accordingly, the use of "about" to modify a numerical value means that the invention is not limited to the precise value. In some instances, the approximating language may correspond to the precision of an instrument for measuring the value. In the present description and claims, range limitations may be combined and/or interchanged, including all sub-ranges contained therein if not otherwise stated.

In addition, the indefinite articles "a" and "an" preceding an element or component of the invention are not intended to limit the number requirement (i.e., the number of occurrences) of the element or component. Thus, "a" or "an" should be read to include one or at least one, and the singular form of an element or component also includes the plural unless the stated number clearly indicates that the singular form is intended.

The present invention is illustrated by the following specific embodiments, but is not limited to the specific examples given below.

The plant extract compound comprises 45-65 parts by weight of rosemary extract, 10-15 parts by weight of galangal extract and 3-5 parts by weight of gallnut extract.

In one embodiment, the plant extract comprises 45-60 parts by weight of rosemary extract, 10-15 parts by weight of galangal extract and 3-5 parts by weight of gallnut extract.

[ plant extract ]

In a preferred embodiment, the rosemary extract of the present invention comprises an alcohol rosemary extract, and the alcohol rosemary extract is prepared by extracting rosemary leaf powder through supercritical carbon dioxide.

The rosemary leaf is the leaf of rosemary. Has tea fragrance, pungent and slightly bitter taste. Contains terpenes, flavone, etc., wherein the terpenes mainly comprise diterpene phenol, and also comprise rosmarinol, carnosol and derivatives. Also contains phenolic components, mainly rosmarinic acid (rosmarinic acid), caffeic acid (coffee acid), chlorogenic acid (chlorogenic acid), etc. In addition, rosemary leaves also contain various fatty acids, linear or branched alkanes and various amino acids. Has strong anti-inflammatory activity, antioxidant activity, immunosuppressive activity and other pharmacological actions such as antithrombotic, nephritis, HIV, depression, pneumonia and bacteriostasis, and the components of the rosemary extract can obviously inhibit allergic inflammation. Rosemary can be classified by chemical nature into eucalyptol rosemary (Rosmarin CT 1, 8-Cineol), camphorrosemary (Rosmarin CT Kampfer), and verbenazone rosemary (Rosmarin CT Verbenon).

Rosemary alcohol extract

In one embodiment, the preparation method of the rosemary extract comprises the following steps:

and (3) extraction: mixing the rosemary leaf powder with a entrainer I, introducing CO ₂ After sequentially carrying out static extraction and circulating extraction, separating to obtain rosemary extract;

concentration: filtering and concentrating the rosemary extract to obtain the rosemary alcohol extract.

Preferably, the weight ratio of the rosemary leaf powder to the first entrainer is 1: (0.5 to 0.8); more preferably, the first entrainer of the present invention is 30 to 40wt% ethanol-water mixture. The 30-40 wt% ethanol-water mixture is the mass percentage of ethanol in the ethanol-water mixture.

Further preferably, in the preparation method of the rosemary alcohol extract, the pressure of static extraction is 6-10 MPa, and the time of static extraction is 0.5-1 h. More preferably, in the preparation method of the rosemary alcohol extract, the pressure of the cyclic extraction is 40-60 MPa, the temperature is 40-50 ℃, and the time is 3-5 h.

In a preferred embodiment, in the method for preparing an alcohol extract of rosemary according to the present invention, a second entrainer is added during the cyclic extraction process; in a more preferred embodiment, the second entrainer is 40 to 50wt% ethanol-water mixture, and the frequency of adding the second entrainer is once per hour, and the weight ratio of the added amount of the second entrainer to the rosemary leaf powder is (0.1 to 0.2).

In a further preferred embodiment, in the preparation method of the rosemary alcohol extract, the separation sequentially comprises a first separation and a second separation, wherein the pressure of the first separation is 8-12 MPa, the temperature is 40-50 ℃, the pressure of the second separation is 4-5 MPa, and the temperature is 35-45 ℃.

In a further preferred embodiment, in the method for preparing an alcoholic rosemary extract according to the present invention, the extraction comprises: mixing the rosemary leaf powder with a entrainer I, introducing CO ₂ Sequentially carrying out static extraction and circular extraction, then sequentially carrying out primary separation and secondary separation, and collecting liquid obtained by the primary separation and the secondary separation to obtain rosemary extract; according to the supercritical carbon dioxide extraction method provided by the invention, the weight ratio of the obtained rosemary extract to the rosemary leaf powder is (1-2): 1.

in a further preferred embodiment, in the preparation method of the rosemary alcohol extract, the temperature for concentration is less than 40 ℃; in a further preferred embodiment, the weight ratio of the rosemary alcohol extract to the rosemary extract is (0.1-0.2): 1. the invention does not limit the filtering material specifically, and can filter out large-particle impurities in the rosemary extract, and the large-particle impurities can be any one of a plastic film, a ceramic film, an anion film and a cation film.

Alpinia officinarum extract

Extracting dried rhizome of Alpinia officinarum Hance of Alpinia officinarum of Zingiberaceae; has effects in warming middle energizer, relieving pain, and promoting microcirculation. The rhizome contains various diphenylheptanes compounds: curcumin (curcumin), dihydrocurcumin (dihydrocurcumin), hexahydrocurcumin (hexahydrocurcumin), octahydrocurcumin (octahydrocurcumin), and flavonoids: galangin (galangin), quercetin (quercetin), kaempferol (kaempferol), kaempferide (kaempferide), isorhamnetin (isorhamnetin), quercetin-5-methyl ether (quercetin-5-methyl ether), galangin-3-methyl ether (galangin-3-methyl ether), and optionally rhamnin (rhamnicitrin) and 7-hydroxy-3,5-dimethoxyflavone (7-hydroxy-3, 5-dimethoxyflavone). And volatile oil, which contains: eucalyptol (1,8-cineole), eugenol (eugenol), limonene (pinene), piperylene (cadinene), and methyl cinnamate (methylcinamate). The root also contains beta-sitosterol-beta-glucoside (beta-sitosterol-beta-glucoside), stigmasterol-beta-glucoside (stigmasterol-beta-glucoside), campesterol-beta-glucoside (campestrol-beta-glucoside).

In one embodiment, the galangal extract of the present invention is prepared from galangal by supercritical carbon dioxide extraction.

Preferably, the preparation method of the galangal extract of the invention comprises the following steps:

and (3) extraction: mixing rhizoma Alpiniae Officinarum with entrainer, introducing CO ₂ After the static extraction and the circular extraction are carried out in sequence, the galangal extract is obtained by separation;

concentration: filtering the rhizoma Alpiniae Officinarum extractive solution, and concentrating to obtain rhizoma Alpiniae Officinarum extract. In the preparation method of the galangal extract, the galangal used is sliced and aired, and the water content is less than 20 wt%.

More preferably, the weight ratio of the galangal to the entrainer III is 1: (1-2); further preferably, the third entrainer is 30-40 wt% ethanol-water mixture.

Still preferably, in the preparation method of the galangal extract, the pressure of static extraction is 9-12 MPa, and the time of static extraction is 0.5-1 h. More preferably, in the preparation method of the rosemary alcohol extract, the pressure of the cyclic extraction is 40-60 MPa, the temperature is 50-60 ℃, and the time is 2-4 h.

In a preferred embodiment, in the preparation method of the galangal extract, a entrainer four is added in the circulating extraction process; in a more preferred embodiment, the fourth entrainer of the present invention is 40 to 50wt% ethanol-water mixture, the frequency of adding the fourth entrainer is once per hour, and the weight ratio of the added amount of the fourth entrainer to the rosemary leaf powder is (0.1 to 0.2): 1.

in a more preferred embodiment, in the preparation method of the galangal extract, the separation sequentially comprises a first separation and a second separation, wherein the pressure of the first separation is 9-12 MPa, the temperature is 50-60 ℃, and the pressure of the second separation is 4-5 MPa, and the temperature is 30-45 ℃.

In a further preferred embodiment, in the preparation method of galangal extract according to the present invention, the extraction comprises: mixing rhizoma Alpiniae Officinarum with entrainer, introducing CO ₂ After the static extraction and the circular extraction are sequentially carried out, the primary separation and the secondary separation are sequentially carried out, and the liquid obtained by the primary separation and the secondary separation is collected to obtain the galangal extract; according to the supercritical carbon dioxide extraction method provided by the invention, the weight ratio of the galangal extract to the galangal is (2-3): 1.

in a further preferred embodiment, in the preparation method of galangal extract according to the present invention, the concentration temperature is less than 40 ℃; in a further preferred embodiment, the weight ratio of the galangal extract to the galangal extract of the invention is (0.1-0.2): 1. the invention does not limit the material for filtration, and can filter large-particle impurities in the galangal extract, and can be any one of plastic membranes, ceramic membranes, anion membranes and cation membranes.

The effective components in rosemary and galangal are generally extracted by hot reflux and then concentrated under reduced pressure, but the problems that the extraction capability is poor, and the effective components are seriously lost and difficult to reach the standard due to long-time heating extraction or concentration exist. The inventors have found that supercritical CO is used ₂ The extraction technology and the filtration and concentration are beneficial to extracting substances with biological activity and thermal instability and maintaining the natural characteristics of the substances, and the extraction and concentration efficiency is high, the speed is high, and no matter existsPollution, simple process, pure color and taste of the extract and the like.

In addition, under the influence of sensitive muscles, itch, tingling, burning, flushing and the like may appear on the skin, and the inventor finds that the rosemary extract and the galangal extract obtained by supercritical carbon dioxide extraction can obtain a compound with various active ingredients such as flavone, rosmarinic acid, diphenyl heptane and the like, can inhibit histamine release of mast cells to achieve the effect of relieving itching, and the anti-inflammatory factor in the compound is also beneficial to controlling inflammatory mediators (such as COX 2) and lipid mediators (such as prostaglandin, leukotriene and platelet activating factor), avoids flushing or erythema caused by the inflammatory mediators or the lipid mediators, has high effects of fading, eliminating freckles and relieving itching, is beneficial to inhibiting burning and stinging sensations and has high safety.

Galla chinensis extract

Galla chinensis, also called encarpium, encarpium decoction, gall, is obtained by parasitizing a leaf or petiole of a leaf of Aphis angustifolia or a leaf of another plant of the same genus of Rhamnaceae family of Aphidae family, stabbing to generate a sac-like polyphyte gall, and drying by baking. The gallnut extract has the activity of inhibiting histamine release and inflammatory factor production and controlling and preventing inflammation and anaphylactic reaction. In one embodiment, the gallnut extract of the present invention is prepared from gallnuts by aqueous extraction.

Preferably, the preparation method of the gallnut extract comprises the following steps:

adding water into nutgall, leaching for 40-90 min at 40-60 ℃ to obtain a leaching solution, decoloring and concentrating to obtain a nutgall extract. More preferably, in the preparation method of the gallnut extract, the weight ratio of the gallnut to the water is 1: (8-12). The decolorization of the present invention can be carried out by physical adsorption or chemical adsorption, but is not particularly limited, and for example, the decolorization is carried out by physical adsorption with polar adsorbents such as silica gel and alumina, or by non-polar adsorbents such as activated carbon, talc powder and diatomaceous earth, or by chemical adsorption with anion exchange resin and basic alumina. In one embodiment, the temperature of the decolorization is 40 to 60 ℃ and the time is 20 to 40 min.

The inventor finds that the plant extract is generally added to water-based daily necessities for use, but the rosemary extract and the galangal extract obtained by supercritical carbon dioxide extraction contain a lot of oil-soluble components, such as flavone, rosmarinic acid, diphenyl heptane and the like, are not favorable for dissolving in water, and can aggregate with residual ethanol and the like in the extract to generate particles with the size of more than 50nm to obtain opaque or semitransparent liquid, and the inventor finds that the reduction of the particle size of the oil-soluble particles is promoted by adding a certain content of the gallnut extract, the components of the gallnut extract, such as tannic acid obtained by condensation of gallic acid and glucose, and the like, and the ethanol and the like, which act together with the rosemary extract and the galangal extract to obtain clear and transparent solution, and the solution is still stable in a high-low temperature state.

In one embodiment, the plant extract of the present invention further comprises 1 to 5 parts by weight of oat extract.

Oat extract

The oat extract is a whole plant extract of the gramineae plant oat, mainly contains beta-glucan, flavonoid compounds and various antioxidant substances, has excellent anti-aging effect, and has the effects of tonifying spleen, stomach, intestine and induced spawning, stopping sweating due to debility, stopping bleeding and the like. Oat is also an ideal food for preventing atherosclerosis, hypertension and coronary heart disease. The fat content is particularly rich and mainly comprises single unsaturated fatty acid, linoleic acid and linolenic acid.

The inventor finds that by adding the oat extract and the rosemary alcohol extract, the galangal extract and the gallnut extract to act together, the active ingredients in the oat extract can be recognized by receptors on immunocompetent cell membranes and activate immunocompetent cells, and the beta-glucan and the like can increase Epidermal Growth Factor (EGF) on aged skin and wrinkled skin, promote collagen and elastin in the skin to increase, promote fibroblasts to synthesize collagen, promote wound healing, repair damaged skin, and promote the effects of relieving itching, diminishing inflammation and fading red. The oat extract is not particularly limited in the invention, and can be made by oneself or purchased.

In addition, the applicant also finds that the galangal extract contains glucoside and other components, and can form a water-insoluble compound with tannic acid and the like, so that the dispersion and dissolution of the extraction compound in water are influenced, and particularly, precipitation and the like can be generated during long-term centrifugation.

In one embodiment, the rosemary extract of the present invention further comprises rosemary leaf water, wherein the rosemary leaf water is prepared from rosemary leaves by water extraction.

Rosemary leaf water

Before carbon dioxide extraction, the inventor finds that essential oil and water-soluble components of the rosemary leaves can be extracted by distillation to obtain rosemary leaf water, a small amount of substances such as 1.8-cineole and the like contained in the rosemary leaf water are beneficial to promoting skin absorption, and the rosemary leaf water and the rosemary alcohol extract are added into a plant extraction compound together to further promote the inhibition of the release of histamine and inflammatory factors and control and prevent inflammation and allergen.

However, the inventor finds that long-time high-temperature distillation causes great loss of active ingredients such as terpenes, flavonoids and rosmarinic acid in rosemary leaves, influences the content of the active ingredients in the carbon dioxide extraction process, and needs to control proper distillation time so as to control the steam quantity of steam within a proper range, so that the water phase in an oil-water mixture accounts for 0.5-0.7 of the mass of raw materials before distillation, and the loss of oil-soluble rosmarinic acid, flavonoids and other substances in residues is avoided while the loss of subsequent oil-water separation is avoided. In one embodiment, the method for preparing rosemary leaf water comprises the following steps:

after the rosemary leaves are crushed, carrying out steam distillation for 20-30 min to obtain an oil-water mixture and powder residues, separating the oil-water mixture to obtain a water phase serving as rosemary leaf water, and drying the powder residues to obtain rosemary leaf powder. Preferably, the weight ratio of the rosemary leaf water to the rosemary leaf is (0.5-0.7): 1. wherein the powder residue is dried and used as rosemary leaf powder for preparing rosemary alcohol extract.

In one embodiment, the weight ratio of the rosemary leaf water to the rosemary extract is (45-55): (5-10); preferably (45-50): (5-10).

In one embodiment, the raw material for preparing the plant extract complex of the present invention further comprises a polyol.

[ polyhydric alcohol ]

The polyhydric alcohol can be used as a humectant to increase the water content of the skin surface, and also has an antibacterial effect, the plant extracts of rosemary, galangal, gallnut, oat and the like with a certain amount provided by the invention act together, and the small molecular alcohol is added to promote bacteriostasis and moisture retention, so that the obtained plant extract compound is beneficial to relieving burning stabbing pain or numbness and itching caused by over-activation of TRPV receptors or over-release of histamine in skin sensing fibers, is beneficial to generating anti-inflammation, lipid medium factors and the like, has the effects of fading red and removing freckles, does not generate greasy feeling after being used, and hardly generates irritation. The polyhydric alcohol is not particularly limited in the present invention, and there may be mentioned glycerin, 1, 3-propanediol, 2, 3-butanediol, 2-methyl-1, 3-propanediol, isoprene glycol and 1, 4-hexanediol. In one embodiment, the weight ratio of the polyhydric alcohol and the plant extract is (1-2): (7-10). Preferably, the weight ratio of the 2, 3-butanediol to the 1, 4-hexanediol in the invention is (5-10): (4-8).

In one embodiment, the raw material for preparing the plant extract complex of the present invention further comprises water.

In a second aspect, the present invention provides a method for preparing the plant extract complex for relieving itching and inflammation, comprising: adding the plant extracts into water, and mixing to obtain the plant extract compound. The plant extraction compound obtained by the invention is clear and transparent.

In one embodiment, the method for preparing a plant extraction complex according to the present invention comprises: adding herba Rosmarini officinalis extract, rhizoma Alpiniae Officinarum extract, and Galla chinensis extract into water, and mixing to obtain the plant extract complex.

In one embodiment, the method for preparing a plant extraction complex according to the present invention comprises: adding rosemary extract, galangal extract, gallnut extract and oat extract into water, and mixing to obtain the plant extraction compound.

In one embodiment, the method for preparing a plant extraction complex according to the present invention comprises: adding rosemary extract, galangal extract, gallnut extract and oat extract into water, mixing, adding rosemary leaf water, and mixing to obtain the plant extract complex. The temperature of the rosemary leaf water added in the invention is 20-40 ℃.

In one embodiment, the method for preparing a plant extraction complex according to the present invention comprises: adding rosemary extract, galangal extract, gallnut extract and oat extract into water, mixing, heating to 60-70 ℃, adding polyol, mixing, cooling to 20-40 ℃, adding rosemary leaf water, and mixing to obtain the plant extraction compound.

The third aspect of the invention provides application of the plant extraction compound for relieving itching and diminishing inflammation, which is used for daily chemicals. Such as anti-inflammatory and allergy-relieving products, itching-relieving products, products for preventing or treating skin inflammation, whitening and sun-screening products and skin injury repairing products. Is suitable for various dosage forms, such as shampoo, hair conditioner, scalp care, body wash, lotion, cream, facial mask, essence, etc.; the suggested dosage is: 0.5-4%; the pH range used was: it is stable in a wide pH range (3-8).

Examples

The present invention will be specifically described below by way of examples. It should be noted that the following examples are only for illustrating the present invention and should not be construed as limiting the scope of the present invention, and that the insubstantial modifications and adaptations of the present invention by those skilled in the art based on the above disclosure are still within the scope of the present invention. Examples and comparative examples oat extracts were obtained from snout biotechnology limited and the rosemary leaves were eucalyptol rosemary leaves.

Example 1

This example provides a method for preparing rosemary leaf water, comprising:

after the rosemary leaves are crushed, carrying out steam distillation for 25min to obtain an oil-water mixture and powder residues, separating the oil-water mixture to obtain a water phase serving as rosemary leaf water, and drying the powder residues to obtain rosemary leaf powder; the weight ratio of the rosemary leaf water to the rosemary leaf is 0.5: 1.

comparative example 1

This example provides a method for preparing rosemary leaf water, comprising:

after the rosemary leaves are crushed, carrying out steam distillation for 1h to obtain an oil-water mixture and powder residues, separating the oil-water mixture to obtain a water phase serving as rosemary leaf water, and drying the powder residues to obtain rosemary leaf powder; the weight ratio of the rosemary leaf water to the rosemary leaf is 1.2: 1.

example 2

This example provides a method for preparing an alcoholic extract of rosemary comprising:

and (3) extraction: taking 1 part of rosemary leaf powder obtained in example 1, adding 0.7 part by mass of 35wt% ethanol-water mixture, uniformly mixing, filling into an extraction kettle, introducing CO ₂ Keeping the pressure at 9MPa for static extraction for 1h, then adjusting the pressure of the extraction kettle to 50MPa, the temperature to 45 ℃, and introducing supercritical CO ₂ Continuously and circularly extracting for 4h, and adding 45wt% ethanol-water mixture once per hour in the circular extractionThe amount of the secondary addition is 0.15 part: after cyclic extraction, sequentially passing through a first separation kettle and a second separation kettle, wherein the pressure of the first separation kettle is 10MPa, the temperature of the first separation kettle is 45 ℃, the pressure of the second separation kettle is 4MPa, and the temperature of the second separation kettle is 40 ℃, and collecting the liquid of the first separation kettle and the liquid of the second separation kettle to obtain 1.1 parts of rosemary extract;

concentration: filtering herba Rosmarini officinalis extract 1 part with ceramic membrane to obtain filtrate 0.9 part, and concentrating at below 40 deg.C to obtain herba Rosmarini officinalis extract 0.1 part.

Comparative example 2

This example provides a method for preparing an alcohol extract of rosemary, comprising:

rosemary leaf powder obtained in example 1 was added with ethanol, and subjected to hot reflux extraction and concentration under reduced pressure to obtain rosemary alcohol extract 0.11 parts.

Example 3

This example provides a method for preparing an extract of galangal comprising:

extraction: slicing and airing galangal to obtain galangal airing tablets with the water content of less than 20%, taking 1 part of galangal airing tablets, adding 30-40 wt% ethanol-water mixture with the mass of 1.5 parts, uniformly mixing, filling into an extraction kettle, introducing CO, and drying in the air ₂ Keeping the pressure at 12MPa for static extraction for 1h, then adjusting the pressure of the extraction kettle to 50MPa, the temperature to 60 ℃, and introducing supercritical CO ₂ Continuously and circularly extracting for 3 hours, wherein in the circular extraction, 0.3 part of 40-50 wt% ethanol-water mixture is added every hour; after circulating extraction, sequentially passing through a first separation kettle and a second separation kettle, wherein the pressure of the first separation kettle is 12MPa, and the temperature is 50 ℃; and collecting the liquid in the first separation kettle and the liquid in the second separation kettle at the pressure of 5MPa and the temperature of 40 ℃ to obtain 2 parts of galangal extract.

Concentration: filtering 1 part of rhizoma Alpiniae Officinarum extractive solution with ceramic membrane to obtain 0.95 part of filtrate, and concentrating at below 40 deg.C to obtain 0.2 part of rhizoma Alpiniae Officinarum extract.

Comparative example 3

This example provides a method for preparing an extract of galangal comprising: slicing rhizoma Alpiniae Officinarum, air drying to obtain rhizoma Alpiniae Officinarum air-dried tablet with water content below 20%, adding ethanol into 1 part of the rhizoma Alpiniae Officinarum air-dried tablet, heat refluxing, and concentrating under reduced pressure to obtain 0.4 part of rhizoma Alpiniae Officinarum extract.

Example 4

The embodiment provides a preparation method of a gallnut extract, which comprises the following steps:

adding 10 parts of water into 1 part of Galla chinensis, leaching at 60 deg.C for 60min, adding active carbon into the obtained leaching solution, stirring at 60 deg.C for decolorizing for 30min, filtering to obtain decolorized filtrate, and vacuum concentrating to obtain brown transparent liquid as Galla chinensis extract.

The extracts prepared by the methods provided in examples 1-3 and comparative examples 1-3 were tested separately and the results were as follows:

(1) the rosmarinic acid content of rosemary leaves before distillation and rosemary leaf powder after distillation in example 1 and comparative example 1 were tested by using an HPLC method, and the results are shown in table 1, and it can be seen that compared with the content of rosmarinic acid before distillation, the content of rosmarinic acid in example 1 is lost by 8.3%, and the content of rosmarinic acid in comparative example 1 is lost by 30%, and the extraction method for rosemary leaves prepared by using the method provided by the present invention can effectively reduce the decomposition and deterioration of unstable components.

TABLE 1

(2) The rosemary alcohol extracts obtained in example 2 and the rosemary alcohol extract obtained in comparative example 2 and the galangal extract obtained in example 3 and the galangal extract obtained in comparative example 3 are respectively used for comparison, and the total content of acid phenols is determined by adopting a spectrophotometry method, and the specific data are as follows:

TABLE 2

From the above table, it can be found that the supercritical carbon dioxide extraction methods provided in examples 2 and 3 can extract substances with biological activity and thermal instability, have higher total content extraction rate of acid and phenol and maintain natural characteristics thereof, and have the advantages of high extraction and concentration efficiency, high speed, no pollution, simple process, pure color and taste of the extract, and the like.

Example 5

The embodiment provides a plant extraction compound, and the plant extraction compound is prepared from the following raw materials in parts by weight: 48 parts of rosemary alcohol extract, 12 parts of galangal extract, 5 parts of gallnut extract and 35 parts of water in balance.

The preparation methods of the rosemary alcohol extract, the galangal rhizome extract and the gallnut extract are respectively shown in examples 2,3 and 4.

This example also provides a method of preparing a plant extract complex as described above, comprising: adding herba Rosmarini officinalis extract, rhizoma Alpiniae Officinarum extract, and Galla chinensis extract into water, and mixing to obtain clear and transparent plant extract complex.

Example 6

The embodiment provides a plant extraction compound, and the plant extraction compound is prepared from the following raw materials in parts by weight: 48 parts of rosemary alcohol extract, 12 parts of galangal extract, 5 parts of gallnut extract, 5 parts of oat extract and 30 parts of water in balance.

This example also provides a method of preparing a plant extract complex as described above, comprising: adding herba Rosmarini officinalis extract, rhizoma Alpiniae Officinarum extract, Galla chinensis extract, and herba Avenae Fatuae extract into water, and mixing to obtain clear and transparent plant extract complex.

Example 7

The embodiment provides a plant extraction compound, and the plant extraction compound is prepared from the following raw materials in parts by weight: 48 parts of rosemary alcohol extract, 10 parts of rosemary leaf water, 12 parts of galangal extract, 5 parts of gallnut extract, 5 parts of oat extract and 20 parts of the balance of water.

The preparation methods of the rosemary leaf water, the rosemary alcohol extract, the galangal rhizome extract and the gallnut extract are respectively shown in examples 1, 2,3 and 4.

This example also provides a method of making a plant extraction complex as described above, comprising: adding herba Rosmarini officinalis extract, rhizoma Alpiniae Officinarum extract, Galla chinensis extract, and herba Avenae Fatuae extract into water, mixing, adding herba Rosmarini officinalis leaf water, and mixing to obtain clear and transparent plant extract complex.

Example 8

The embodiment provides a plant extraction compound, and the plant extraction compound is prepared from the following raw materials in parts by weight: 48 parts of rosemary alcohol extract, 10 parts of rosemary leaf water, 12 parts of galangal extract, 5 parts of gallnut extract, 5 parts of oat extract, 6 parts of 2, 3-butanediol, 4 parts of 1, 4-hexanediol and the balance of water 10 parts.

This example also provides a method of making a plant extraction complex as described above, comprising: adding herba Rosmarini officinalis extract, rhizoma Alpiniae Officinarum extract, Galla chinensis extract, and herba Avenae Fatuae extract into water, mixing, heating to 70 deg.C, adding 2, 3-butanediol and 1, 4-hexanediol, mixing, cooling to 40 deg.C, adding herba Rosmarini officinalis leaf water, and mixing to obtain clear and transparent plant extract compound.

The plant extraction compound provided in examples 5-8 was tested for efficacy, with the following results:

(1) mast cell stabilization of the extract complex (anti-allergic antipruritic efficacy)

The mechanism is as follows: based on the C48/80 (Compound 48/80) induced mast cells, an in vitro cell degranulation model is established, whether a sample has stable mast cells to inhibit histamine release to achieve the effect of relieving allergy is evaluated, whether the sample has stable mast cells to inhibit histamine release to achieve the effect of relieving allergy and relieving itching is evaluated, and the test method is shown in the table 3:

TABLE 3

The results of measuring the degranulation rate of mast cells are shown in Table 4 and FIG. 1.

TABLE 4

From the test results of the degranulation rate of the mast cells in table 3 and fig. 1, it can be seen that the P values of the extraction complexes provided in examples 5 to 8 are all less than 0.001, which indicates that the extraction complex provided by the invention can obviously inhibit the degranulation amount generated by stimulating mast cells by C48/80, and has good allergy-relieving and itching-relieving effects.

(2) Anti-inflammatory and allergy-relieving test of 3D skin model of extracted complex

The test method comprises the following steps: A3D skin model (EpiKutis) is stimulated by sodium dodecyl sulfate (SLS), and an 'SLS-EpiKutis' skin damage model is constructed by simulating a human body patch test. Meanwhile, a surface administration mode is adopted, samples with different concentration gradients are used for simulating the use process of a human body, are uniformly coated on the surface of an SLS-EpiKutis skin injury model constructed by an experiment, and the anti-inflammatory and allergy-relieving effects of the sample to be tested are evaluated by detecting the secretion of inflammatory factors of the model, wherein test parameters and detection indexes are shown in a table 5.

TABLE 5

The secretion of inflammatory factors was detected using a Human ELISA kit, three tests were performed for each group to obtain three series of inflammatory factor concentrations, and the average concentration and SD were calculated, with the results shown in table 6 and fig. 2:

TABLE 6

As can be seen from table 5 and fig. 2, the NC group showed a significant up-regulation of inflammatory factor concentration (p < 0.01) under SLS compared to the BC group. The concentration of inflammatory factors was significantly suppressed in the PC group compared to the NC group (p < 0.01). The

samples

8, 7, 6 and 5 in the examples show the inhibition effect (p < 0.01) on the inflammatory factors under the working concentration of 5mg/ml, the effect is better than that of dipotassium glycyrrhizinate, and the effect of the working concentration of the example 8 is better than that of dexamethasone.

(3) Stability testing of extraction complexes

And (3) heat resistance test: the plant extraction compound provided in examples 5 to 8 was placed in an electric-heating constant temperature incubator at 50. + -.1 ℃ for 24 hours, and after the temperature was returned to room temperature, the appearance was observed to be changed, and it was found that none of examples 5 to 8 was changed.

Cold resistance test: the plant extraction compound provided by the embodiment 5-8 is placed in a refrigerator at the temperature of minus 5 to minus 10 +/-1 ℃ for 24 hours, and the appearance is observed to be changed after the room temperature is recovered, so that no change is found in the embodiment 5-8.

And (3) centrifugal test: the plant extraction compound provided in examples 5 to 8 was placed in a centrifuge, and tested at a rotation speed of 2000 to 4000 r/min for 30min, 2h, and 6h to see if the appearance changed, the results are shown in table 7. Centrifuging for 2h and 6 h.

TABLE 7

(4) Safety testing of the extracted complexes: the following toxicology tests were carried out on the extraction complexes provided in examples 5-8, respectively: the skin irritation/corrosiveness test (RHCE test), the acute eye irritation/corrosiveness test (BCOP test), the skin allergy test (DPRA test), the human skin patch test, and the results are shown in table 8.

TABLE 8

(5) Human skin trial test of extracted complexes

The extracted compound provided in example 8 and the contests 1 and 2 1 are diluted with purified water to obtain application test products with the content of 2%, the application test products are respectively numbered as No. 1, No. 2 and No. 3, the contests 1 and 2 are similar efficacy products sold in the market, and the anti-inflammatory and antipruritic effects of the test products are judged in a manner of blind testing by volunteers.

The test method comprises the following steps: using a skin prick test model, 11 volunteers are selected and coded as A-K, wherein 3 volunteers with sensitive skin are numbered as I, J, K, histamine hydrochloride (10 mg/ml) is dripped on skin test parts of the volunteers, after 5 minutes, the skin can generate red swelling and itching, and example 8 and competitive products 1 and 2 (2 mg/cm) with the content of 2% are respectively dripped on the test parts ² ) As the test article, the itching feeling, the change in the degree of redness and swelling, and the skin feeling at the test site were measured within 30 minutes after the application of the test article.

Test determination parameters:

itch feeling: subjective sensory score of subjects (set on a scale of 1-5, with 5 representing the most intense itch);

change in degree of redness and swelling: visual scoring (setting a grade of 1-5, 5 representing the largest red swelling area);

skin feel: comfort (fine, refreshing and greasy), stabbing pain (with prickles and slight pricks) and burning (hot and hot).

The results of the human skin test are shown in Table 9, the average scores of itch and red swelling of the human skin test are shown in FIG. 3, and the picture of the human skin test is shown in FIG. 4.

TABLE 9

As can be seen from the blind test results of the example 8 and the competitive products on the skin of a human body, which are provided in the table 8 and the figure 3, the sample of the example 8 has relatively outstanding effects of soothing the skin, resisting inflammation and relieving itching, and the effect is better than that of the similar competitive products. It can be found from fig. 4 that the skin becomes flush after histamine is tried on the left side of fig. 4, and the skin becomes faded obviously after histamine is tried on the right side of fig. 4 and example 8 is used, which also shows that the extraction compound provided by the invention has the effects of rapid inflammation diminishing and fading.

(6) Bacteriostatic efficacy of the extracted complex

The extracted complexes provided in example 8 were tested for bacteriostasis and the results are shown in table 10.

Watch 10

Example 9

This example provides the use of a plant extract complex for skin care products formulated in mass percent as shown in table 11, with the exception of the plant extract complex, all materials commercially available.

TABLE 11

The present example also provides a method of preparing a skin care product as described above, comprising: mixing water and propylene glycol, adding A, B phase rest raw materials, stirring to dissolve completely, heating to 70 deg.C, stirring, maintaining the temperature, and stirring for 20min to obtain water phase; heating the phase D to 70 ℃, and uniformly stirring to prepare an oil phase; slowly adding the oil phase into the water phase, emulsifying by using a homogenizer, cooling to 40 ℃, adding the phase C, adjusting the pH value to 5.5-6.5, and obtaining the cream in milky white as a skin care product.

The skin care product obtained in example 9 was subjected to a stability test, and the test results were: the temperature is 40-50 ℃, and the room temperature is recovered for 72 hours without change; the temperature is between 20 ℃ below zero and 0 ℃, and the room temperature is recovered for 72 hours without change; cream with milky white color and no demixing at 5-15 deg.C.

According to test results, the plant extraction compound provided by the invention can efficiently inhibit histamine release, has the effects of anti-allergy and itching relieving, has good anti-inflammatory, detumescence and red fading effects and certain bacteriostatic effect, can be used in various daily chemicals, and is extracted by natural plants, free of side effect, mild and moist.

The foregoing examples are merely illustrative and serve to explain some of the features of the method of the present invention. The appended claims are intended to claim as broad a scope as can be conceived and the examples presented herein are merely illustrative of selected implementations in accordance with all possible combinations of examples. Accordingly, it is applicants' intention that the appended claims are not to be limited by the choice of examples illustrating features of the invention. Also, where numerical ranges are used in the claims, subranges therein are included, and variations in these ranges are also to be construed as possible being covered by the appended claims.

Claims

1. The plant extraction compound is characterized in that the plant extraction compound is prepared from raw materials including, by weight, 45-65 parts of rosemary extract, 10-15 parts of galangal extract, 3-5 parts of gallnut extract and 1-5 parts of oat extract;

the rosemary extract comprises rosemary alcohol extract, and the rosemary alcohol extract is prepared by rosemary leaf powder through supercritical carbon dioxide extraction; the rosemary extract also comprises rosemary leaf water, and the rosemary leaf water is prepared from rosemary leaves through water extraction; the preparation method of the rosemary leaf water comprises the following steps:

after the rosemary leaves are crushed, carrying out steam distillation for 20-30 min to obtain an oil-water mixture and powder residues, separating the oil-water mixture to obtain a water phase serving as rosemary leaf water, and drying the powder residues to obtain rosemary leaf powder;

the galangal extract is prepared from galangal by supercritical carbon dioxide extraction;

the gallnut extract is prepared by extracting gallnut with water.

2. The anti-itch anti-inflammatory plant extract complex according to claim 1, further comprising water.

3. A method for preparing the plant extract complex for relieving itching and inflammation according to claim 2, comprising: adding the plant extracts into water, and mixing to obtain the plant extract compound.

4. Use of the plant extract composition for relieving itching and inflammation according to any one of claims 1 to 2, which is used for daily chemicals.