CN113475663A - Peptide-containing probiotic composition, peptide-containing probiotic solid beverage and preparation method thereof - Google Patents

Peptide-containing probiotic composition, peptide-containing probiotic solid beverage and preparation method thereof Download PDF

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CN113475663A
CN113475663A CN202110823392.1A CN202110823392A CN113475663A CN 113475663 A CN113475663 A CN 113475663A CN 202110823392 A CN202110823392 A CN 202110823392A CN 113475663 A CN113475663 A CN 113475663A
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peptide
parts
rapeseed
containing probiotic
cottonseed
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徐力
郭轶
王紫燕
于书萱
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Jilin University
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Jilin University
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    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
    • A23L2/385—Concentrates of non-alcoholic beverages
    • A23L2/39—Dry compositions
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
    • A23L2/52—Adding ingredients
    • A23L2/60—Sweeteners
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/135—Bacteria or derivatives thereof, e.g. probiotics
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/17—Amino acids, peptides or proteins
    • A23L33/18—Peptides; Protein hydrolysates
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/20—Reducing nutritive value; Dietetic products with reduced nutritive value
    • A23L33/21—Addition of substantially indigestible substances, e.g. dietary fibres
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2400/00—Lactic or propionic acid bacteria
    • A23V2400/11—Lactobacillus
    • A23V2400/113—Acidophilus
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2400/00—Lactic or propionic acid bacteria
    • A23V2400/51—Bifidobacterium
    • A23V2400/533—Longum

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  • Engineering & Computer Science (AREA)
  • Food Science & Technology (AREA)
  • Polymers & Plastics (AREA)
  • Mycology (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
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Abstract

The invention provides a peptide-containing probiotic composition, a peptide-containing probiotic solid beverage and a preparation method thereof, belonging to the technical field of health care products, wherein the peptide-containing probiotic composition comprises the following components in parts by weight: 20-30 parts of lactobacillus acidophilus, 20-30 parts of bifidobacterium longum, 21-26 parts of rapeseed peptide and 21-26 parts of cottonseed peptide; the peptide-containing probiotic solid beverage comprises 82-122 parts of the peptide-containing probiotic composition, 25-30 parts of resistant dextrin, 4-8 parts of maltodextrin and 18-22 parts of fructo-oligosaccharide. The cottonseed peptide and the rapeseed peptide in the peptide-containing probiotic composition provided by the invention can obviously promote the proliferation of lactobacillus acidophilus and bifidobacterium longum, and all components in the peptide-containing probiotic composition are mutually synergistic, so that the probiotic effect can be better exerted, the intestinal microecological balance of a human body can be adjusted, and the symptoms such as constipation, diarrhea and the like caused by the imbalance of intestinal flora can be relieved.

Description

Peptide-containing probiotic composition, peptide-containing probiotic solid beverage and preparation method thereof
Technical Field
The invention belongs to the technical field of health care products, and particularly relates to a peptide-containing probiotic composition, a peptide-containing probiotic solid beverage and a preparation method thereof.
Background
The probiotics is an active microorganism which is beneficial to a host and is formed by changing the flora at a certain part of the host through colonization in a human body, and the function of promoting nutrient absorption and keeping the intestinal tract healthy is realized by regulating the immune function of the host mucous membrane and the system or regulating the flora balance in the intestinal tract, so that a single microorganism which is beneficial to the health or a mixed microorganism with a definite composition is generated. The probiotics can reach the small intestine and the large intestine, inhibit the propagation of harmful bacteria in the intestine and promote the intestinal tract movement, thereby improving the intestinal tract function and the defecation condition and playing an important balance role in the whole body health and the immune system.
The probiotic products on the market at present have single functional components, and the combination of probiotics and natural bioactive peptides does not exist at present.
Disclosure of Invention
In view of the above, the present invention aims to provide a peptide-containing probiotic composition, a peptide-containing probiotic solid beverage and a preparation method thereof; the peptide-containing probiotic composition comprises cottonseed peptide and rapeseed peptide; the cottonseed peptide and the rapeseed peptide can promote the proliferation of lactobacillus acidophilus and bifidobacterium longum.
In order to achieve the above purpose, the invention provides the following technical scheme:
the invention provides a peptide-containing probiotic composition, which comprises the following components in parts by weight: 20-30 parts of lactobacillus acidophilus, 20-30 parts of bifidobacterium longum, 21-26 parts of rapeseed peptide and 21-26 parts of cottonseed peptide.
Preferably, the composition comprises the following components in parts by weight: 23-27 parts of lactobacillus acidophilus, 23-27 parts of bifidobacterium longum, 23-24 parts of rapeseed peptides and 23-24 parts of cottonseed peptides.
Preferably, the preparation method of the rapeseed peptide comprises the following steps:
A1) mixing rapeseed cake meal with water to obtain rapeseed suspension;
A2) mixing the rapeseed suspension with alkaline protease, and carrying out enzymolysis to obtain an enzymolysis solution;
A3) after the pH value of the enzymolysis liquid is adjusted to be neutral, centrifuging, filtering and collecting filtrate to obtain rapeseed peptide;
the preparation method of the cottonseed peptide comprises the following steps:
B1) mixing cottonseed cake meal with water to obtain cottonseed suspension;
B2) mixing the cottonseed suspension with alkaline protease, and carrying out enzymolysis to obtain an enzymolysis solution;
B3) and adjusting the pH value of the enzymolysis solution to be neutral, centrifuging, filtering, and collecting filtrate to obtain the cottonseed peptide.
Preferably, the mass volume ratio of the rapeseed cake and the water in the step A1) is 1g (8-12 ml), and the mass volume ratio of the cottonseed cake and the water in the step B1) is 1g (8-12 ml);
preferably, the temperature of the enzymolysis in the step A2) or the step B2) is 48-52 ℃, the enzymolysis process is accompanied by oscillation, the pH value of the enzymolysis feed liquid is adjusted to 8.0 once every 8-12 min in the enzymolysis process, and the enzymolysis is finished when the pH value of the enzymolysis feed liquid is maintained at 8.0 and does not change along with the enzymolysis.
The invention provides a peptide-containing probiotic solid beverage which comprises the following components in parts by weight: 82-122 parts of peptide-containing probiotic composition, 25-30 parts of resistant dextrin, 4-8 parts of maltodextrin and 18-22 parts of fructo-oligosaccharide.
Preferably, the composition comprises the following components in parts by weight: 24-26 parts of lactobacillus acidophilus, 24-26 parts of bifidobacterium longum, 23-24 parts of rapeseed peptide, 23-24 parts of cottonseed peptide, 26-28 parts of resistant dextrin, 5-7 parts of maltodextrin and 19-21 parts of fructo-oligosaccharide.
The invention provides a preparation method of a peptide-containing probiotic solid beverage, which comprises the following steps:
1) mixing rapeseed peptide, cottonseed peptide, resistant dextrin, maltodextrin, fructo-oligosaccharide and water to obtain a mixed material;
2) freeze-drying the mixed material to obtain a semi-finished product of the solid beverage;
3) and mixing the semi-finished solid beverage, lactobacillus acidophilus and bifidobacterium longum to obtain the peptide-containing probiotic solid beverage.
Preferably, stirring is carried out in the mixing process in the step 1), and the stirring time is 20-30 min.
Preferably, the mixing process in the step 3) is accompanied by stirring, and the stirring time is 30-40 min.
The invention has the beneficial effects that: the peptide-containing probiotic composition provided by the invention comprises cottonseed peptide and rapeseed peptide; the cottonseed peptide and the rapeseed peptide can remarkably promote the proliferation of lactobacillus acidophilus and bifidobacterium longum, and the rapeseed peptide and the cottonseed peptide belong to natural micromolecule active peptides and have a certain antioxidant effect; the components in the peptide-containing probiotic composition are synergistic, so that the probiotic effect can be better exerted, the micro-ecological balance of human intestinal tracts can be adjusted, and the symptoms of constipation, diarrhea and the like caused by intestinal flora imbalance can be relieved.
The peptide-containing probiotic solid beverage provided by the invention comprises fructo-oligosaccharide which is used as a prebiotic, not only can promote the proliferation of intestinal probiotics, but also can replace a common sweetener, and effectively reduces the influence of sugar in the solid beverage on the health of a human body.
Drawings
FIG. 1 shows the growth of Lactobacillus acidophilus promoted by rapeseed peptides of different concentrations;
FIG. 2 shows the growth of Lactobacillus acidophilus promoted by cottonseed peptide at different concentrations;
FIG. 3 shows the growth of Bifidobacterium longum promoted by rapeseed peptides at different concentrations;
FIG. 4 shows the growth of Bifidobacterium longum promoted by cottonseed peptide at different concentrations.
Detailed Description
The invention provides a peptide-containing probiotic composition, which comprises the following components in parts by weight: 20-30 parts of lactobacillus acidophilus, 20-30 parts of bifidobacterium longum, 21-26 parts of rapeseed peptide and 21-26 parts of cottonseed peptide. In the invention, the peptide-containing probiotic composition comprises 20-30 parts of lactobacillus acidophilus, preferably 23-27 parts, more preferably 24-26 parts, and most preferably 25 parts. In the invention, the lactobacillus acidophilus is preferably lactobacillus acidophilus freeze-dried powder, and the viable count of the lactobacillus acidophilus freeze-dried powder is preferably more than or equal to 100 hundred million CFU/g; the source of the lactobacillus acidophilus freeze-dried powder is not particularly limited, and the lactobacillus acidophilus freeze-dried powder is obtained by adopting conventional commercial products in the field or self-preparation.
In the invention, the peptide-containing probiotic composition comprises 20-30 parts of bifidobacterium longum, preferably 23-27 parts, more preferably 24-26 parts, and most preferably 25 parts. In the invention, the bifidobacterium longum is preferably bifidobacterium longum freeze-dried powder, and the viable count of the bifidobacterium longum freeze-dried powder is preferably more than or equal to 100 hundred million CFU/g; the source of the bifidobacterium longum lyophilized powder is not particularly limited, and the bifidobacterium longum lyophilized powder is obtained by adopting a conventional commercial product or self-preparation in the field.
In the invention, the peptide-containing probiotic composition comprises 21-26 parts of rapeseed peptides, preferably 23-24 parts of rapeseed peptides, and more preferably 23.5 parts of rapeseed peptides.
In the present invention, the method for producing rapeseed peptide preferably comprises the steps of: A1) mixing rapeseed cake meal with water to obtain rapeseed suspension; A2) mixing the rapeseed suspension with alkaline protease, and carrying out enzymolysis to obtain an enzymolysis solution; A3) and adjusting the pH value of the enzymolysis solution to be neutral, filtering, and collecting filtrate to obtain the rapeseed peptide.
Mixing rapeseed cake meal with water to obtain rapeseed suspension; the mass volume ratio of the rapeseed cake and water is preferably 1g (8-12 ml), more preferably 1g (9-11 ml), and most preferably 1g:10 ml. In the present invention, the water is preferably high purity water. In the invention, stirring is carried out in the mixing process, the rotating speed and the stirring time of the stirring are not particularly limited, and the uniform mixing can be realized. According to the invention, after the mixing, the preheating is preferably carried out, the preheating temperature is preferably 45-55 ℃, more preferably 50 ℃, and the preheating time is preferably 10-20 min, more preferably 15 min; the preheating is carried out by adopting a water bath. After the preheating, the method preferably further comprises a pH value adjusting step, wherein the pH value of the rapeseed suspension is adjusted to 8.0, the reagent for adjusting the pH value is not particularly limited, and an alkaline solution which is conventional in the field is adopted, and in the specific implementation process of the method, a NaOH solution is preferably adopted; the concentration of the NaOH solution is preferably 0.4-0.6 mol/ml, and more preferably 0.5 mol/ml.
In the invention, the rapeseed suspension is mixed with alkaline protease and subjected to enzymolysis to obtain an enzymolysis solution. In the invention, the alkaline protease is preferably preheated, the preheating time is not particularly limited, and the alkaline protease is preheated to 45-55 ℃. In the invention, the volume-to-mass ratio of the alkaline protease to the original rapeseed cake is preferably 1.5ml (8-12 g), and more preferably 1.5ml to 10 g. In the invention, the enzymolysis temperature is preferably 48-52 ℃, and more preferably 50 ℃; the enzymolysis process is accompanied by oscillation, the pH value is adjusted once every 8-12 min, and the preferable enzyme is adjusted once in 10 min; preferably, the pH value of the enzymolysis feed liquid is adjusted to 8.0, and when the pH value of the enzymolysis feed liquid maintains 8.0 and does not change along with the progress of enzymolysis, the enzymolysis is finished. After the enzymolysis is finished, the method preferably further comprises a step of enzyme deactivation, wherein the enzyme deactivation is preferably to place the enzymolysis liquid in a boiling water bath for 10-20 min, and more preferably 15 min.
After the enzyme is inactivated, the pH value of the enzymatic hydrolysate is adjusted to be neutral, and the rapeseed peptide is obtained by centrifuging, filtering and collecting filtrate. In the present invention, the pH is preferably adjusted to 6.9 to 7.1, and more preferably to 7.0. In the invention, the pH value is preferably adjusted by using a citric acid solution, and the concentration of the citric acid solution is preferably 0.4-0.6 mol/ml, and more preferably 0.5 mol/ml. The pH value of the enzymolysis liquid is adjusted to be neutral and then the enzymolysis liquid is centrifuged, wherein the rotation speed of the centrifugation is preferably 7000-8000 rpm, more preferably 7200-7800 rpm, and most preferably 7500 rpm; the time for centrifugation is preferably 10-20 min, and more preferably 15 min. According to the invention, after the centrifugation, the supernatant is collected, and the supernatant is filtered, wherein the filtration is preferably suction filtration, and the aperture of the filter membrane for suction filtration is preferably 0.22 μm. In the present invention, the filtrate obtained by the suction filtration is preferably lyophilized to obtain the rapeseed peptide lyophilized powder, and the specific operation of the lyophilization is not particularly limited in the present invention, and the conventional lyophilization operation in the art may be adopted.
In the invention, the preparation method of the cottonseed peptide comprises the following steps: B1) mixing cottonseed cake meal with water to obtain cottonseed suspension; B2) mixing the cottonseed suspension with alkaline protease, and carrying out enzymolysis to obtain an enzymolysis solution; B3) and adjusting the pH value of the enzymolysis solution to be neutral, centrifuging, filtering, and collecting filtrate to obtain the cottonseed peptide. In the present invention, the specific preparation method of the cottonseed peptide is the same as the preparation method of the rapeseed peptide, and is not described herein again.
The invention provides a peptide-containing probiotic solid beverage which comprises the following components in parts by weight: 82-122 parts of peptide-containing probiotic composition, 25-30 parts of resistant dextrin, 4-8 parts of maltodextrin and 18-22 parts of fructo-oligosaccharide.
In the invention, the peptide-containing probiotic solid beverage comprises the following components in parts by weight: 82-122 parts of the peptide-containing probiotic composition, wherein the peptide-containing probiotic composition specifically comprises 20-30 parts of lactobacillus acidophilus, 20-30 parts of bifidobacterium longum, 21-26 parts of rapeseed peptides and 21-26 parts of cottonseed peptides.
In the invention, the peptide-containing probiotic solid beverage comprises 25-30 parts of resistant dextrin, preferably 26-28 parts of resistant dextrin, and more preferably 27 parts of resistant dextrin, the source and the preparation method of the resistant dextrin are not particularly limited, the peptide-containing probiotic solid beverage is obtained by adopting conventional commercial products or self-preparation in the field, and the peptide-containing probiotic solid beverage is purchased from Wanbang of Henan, Inc. in the specific implementation process of the peptide-containing probiotic solid beverage, and the resistant dextrin is used for supplementing dietary fibers in the solid beverage.
In the invention, the peptide-containing probiotic solid beverage comprises 4-8 parts of maltodextrin, preferably 5-7 parts of maltodextrin, and more preferably 6 parts of maltodextrin; the source and the preparation method of the maltodextrin are not specially limited, the maltodextrin is obtained by adopting conventional commercial products or self-preparation in the field, the maltodextrin is purchased from Wanbang practice Co., Ltd in Henan in the specific implementation process of the invention, and the maltodextrin has the functions of ensuring that the product is not easy to agglomerate and is instant and has good reconstitution property.
In the invention, the peptide-containing probiotic solid beverage comprises 18-22 parts of fructo-oligosaccharide, preferably 19-21 parts of fructo-oligosaccharide, and more preferably 20 parts of fructo-oligosaccharide. The source and the preparation method of the fructo-oligosaccharide are not particularly limited, the fructo-oligosaccharide is obtained by adopting conventional commercial products or self-preparation in the field, the fructo-oligosaccharide is purchased from Wanbang practice Co., Ltd, Henan, and the like in the specific implementation process of the fructo-oligosaccharide serving as a prebiotic, not only can promote the proliferation of intestinal probiotics, but also can replace a common sweetener, and the influence of sugar in the solid beverage on the health of a human body is effectively reduced.
The invention provides a preparation method of a peptide-containing probiotic solid beverage, which comprises the following steps: 1) mixing rapeseed peptide, cottonseed peptide, resistant dextrin, maltodextrin, fructo-oligosaccharide and water to obtain a mixed material; 2) freeze-drying the mixed material to obtain a semi-finished product of the solid beverage; 3) and mixing the semi-finished solid beverage, lactobacillus acidophilus and bifidobacterium longum to obtain the peptide-containing probiotic solid beverage.
In the invention, rapeseed peptide, cottonseed peptide, resistant dextrin, maltodextrin, fructo-oligosaccharide and water are mixed to obtain a mixed material. In the invention, the amount of the water is preferably 200mL in terms of 3g of solid material per 3g of solid material; the temperature of the water is preferably 25-37 ℃. In the invention, stirring is carried out in the mixing process, the stirring time is preferably 20-30 min, and the stirring speed is not particularly limited; in the present invention, the temperature of the mixing is preferably below 37 ℃; in the specific implementation process of the invention, preferably, the rapeseed peptide, the cottonseed peptide, the resistant dextrin, the maltodextrin and the fructo-oligosaccharide are mixed firstly, and then are mixed with water.
In the invention, the mixed material is freeze-dried to obtain a semi-finished solid beverage. In the invention, the temperature of the freeze-drying is preferably-45 ℃, the freeze-drying time is determined according to the volume of the materials, and the freeze-drying time is not less than 12 hours per 100mL of the mixed material solution.
In the invention, the semi-finished product of the solid beverage, the lactobacillus acidophilus and the bifidobacterium longum are mixed to obtain the peptide-containing probiotic solid beverage. In the invention, the mixing process is accompanied by stirring, the stirring time is preferably 30-40 min, the stirring speed is not particularly limited, and the mixing can be realized. After the peptide-containing probiotic solid beverage is obtained, the peptide-containing probiotic solid beverage is preferably packaged; in the implementation process of the invention, the packaging is preferably carried out by using an automatic subpackaging device, and the specific specification is preferably 3 g/bag.
The technical solutions provided by the present invention are described in detail below with reference to examples, but they should not be construed as limiting the scope of the present invention.
Example 1
Peptide-containing probiotic composition:
25 parts of lactobacillus acidophilus freeze-dried powder (from Jiangsu Yuanshen health industry Limited company, the number of living bacteria is more than or equal to 100 hundred million cfu/g), 25 parts of bifidobacterium longum freeze-dried powder (from Jiangsu Yuanshen health industry Limited company, the number of living bacteria is more than or equal to 100 hundred million cfu/g), 23 parts of rapeseed peptide and 23 parts of cottonseed peptide.
The method comprises the following specific steps: 0.5g of lactobacillus acidophilus, 0.5g of bifidobacterium longum, 0.47g of rapeseed peptide and 0.47g of cottonseed peptide.
Peptide-containing probiotic solid beverage: 25 parts of lactobacillus acidophilus freeze-dried powder, 25 parts of bifidobacterium longum freeze-dried powder, 23 parts of rapeseed peptides, 23 parts of cottonseed peptides, 27 parts of resistant dextrin, 6 parts of maltodextrin and 21 parts of fructo-oligosaccharide, and the preparation method comprises the following steps:
0.5g of lactobacillus acidophilus, 0.5g of bifidobacterium longum, 0.47g of rapeseed peptide, 0.47g of cottonseed peptide, 0.54g of resistant dextrin, 0.12g of maltodextrin and 0.42g of fructo-oligosaccharide.
The preparation method of the peptide-containing probiotic solid beverage comprises the following steps:
s1, placing rapeseed peptide, cottonseed peptide, resistant dextrin, maltodextrin and fructo-oligosaccharide into a container, adding sterile water while stirring, and stirring for 5min at room temperature to obtain a mixture.
S2, carrying out vacuum freeze drying treatment on the mixed material prepared in the step S1 for more than 12h at the temperature of minus 45 ℃ to prepare a semi-finished product of the solid beverage.
S3, placing the semi-finished product of the solid beverage prepared in the step S2 into a stirring device, simultaneously placing 25 parts of lactobacillus acidophilus lyophilized powder and 25 parts of bifidobacterium longum lyophilized powder into the stirring device, and stirring for 30min at normal temperature to obtain the probiotic solid beverage.
And S4, dividing the probiotic solid beverage prepared in the step S3 into small packages of 3 g/bag by using an automatic subpackaging device, packaging into large packages according to the product quantity and specification requirements, performing spot check and inspection on finished products according to enterprise standards, and finally warehousing to wait for subsequent spot check work.
Example 2
Peptide-containing probiotic composition:
25 parts of lactobacillus acidophilus freeze-dried powder, 25 parts of bifidobacterium longum freeze-dried powder, 23.5 parts of rapeseed peptide and 23.5 parts of cottonseed peptide.
Peptide-containing probiotic solid beverage: 25 parts of lactobacillus acidophilus freeze-dried powder, 25 parts of bifidobacterium longum freeze-dried powder, 23.5 parts of rapeseed peptides, 23.5 parts of cottonseed peptides, 27 parts of resistant dextrin, 6 parts of maltodextrin and 20 parts of fructo-oligosaccharide.
The preparation method of the peptide-containing probiotic solid beverage is described in example 1.
Example 3
Peptide-containing probiotic composition:
25 parts of lactobacillus acidophilus freeze-dried powder, 25 parts of bifidobacterium longum freeze-dried powder, 24 parts of rapeseed peptide and 24 parts of cottonseed peptide.
Peptide-containing probiotic solid beverage:
25 parts of lactobacillus acidophilus freeze-dried powder, 25 parts of bifidobacterium longum freeze-dried powder, 24 parts of rapeseed peptides, 24 parts of cottonseed peptides, 27 parts of resistant dextrin, 6 parts of maltodextrin and 19 parts of fructo-oligosaccharide.
The preparation method of the peptide-containing probiotic solid beverage is described in example 1.
The methods for producing rapeseed peptides and cottonseed peptides in examples 1 to 3 are as follows:
adding 10g of rapeseed cake meal into 100ml of high-purity water, stirring to form a suspension, placing the suspension in a 50 ℃ water bath for preheating for 15min, taking out the suspension, adding 0.5mol/ml of NaOH solution to adjust the pH value to 8, adding 1.5ml of alkaline protease preheated at the same time, placing the suspension in a 50 ℃ water bath for shaking and enzymolysis, and taking out the suspension after 10min of shaking until the pH value is kept to be 8 and is not changed any more until the pH value is kept to be 8, wherein the enzymolysis is considered to be finished. After the enzymolysis is finished, the mixture is placed in a boiling water bath for 15min to inactivate alkaline protease in the mixture, after the temperature of the mixture is reduced to room temperature, the pH value of the mixture is adjusted to 7 by using 0.5mol/ml citric acid solution, the mixture is placed into a centrifuge tube and centrifuged at 7500rpm for 15min, a supernatant is taken and filtered by a 0.22 mu m filter membrane, and the filtered solution is subjected to vacuum freeze drying (-45 ℃ for more than 12 h), so that rapeseed peptide powder is obtained.
The preparation method of the cottonseed peptide is consistent with that of the rapeseed peptide.
Mouthfeel test of probiotic solid beverage prepared in examples 1 to 3
First, selection and evaluation method of evaluation personnel
The evaluation group consisted of 10 panelists who were healthy, had no adverse tastes such as smoking and alcohol abuse, and had strong discriminative power and high sensitivity for color, flavor and taste. All samples were coded with three random numbers and evaluated at room temperature. Rinse with warm water before sensory evaluation to ensure a fresh mouth, and do not evaluate when there is an emotional impact. Product evaluation domain: when sensory evaluation is performed on food, several indexes that most reflect the quality of the food are often selected as domains U. The experiment confirms that the product quality is composed of 4 factors of color, sweetness, acidity, fragrance and mouthfeel, namely, the index domain U of an evaluation object is equal to (color, sweetness, acidity, fragrance and mouthfeel). Comment level domain: the evaluation of each factor was rated in four grades of good, medium, and poor, i.e., rating domain V ═ good, medium, and poor. Each factor is 20 points and full points are 100 points.
The sensory evaluation reference standard is shown in table 1, the sensory evaluation result is shown in table 2, and the sensory evaluation result is shown in table 3; it can be seen that the peptide-containing probiotic beverage provided by the invention has excellent color, sweetness, acidity, fragrance and taste.
TABLE 1 sensory evaluation reference standards
Figure BDA0003172682160000091
TABLE 2 sensory Scoring results
Sweetness (20 points) Acidity (20 points) Fragrance (20 points) Mouthfeel (20 points) Color and luster (20 points)
Example 1 17 19.6 15.7 15.8 17.9
Example 2 17.7 19.8 17.8 16.1 18.2
Example 3 18.5 19.6 18.1 15.7 18.5
TABLE 3 evaluation results table
Figure BDA0003172682160000092
Figure BDA0003172682160000101
Example 4
Rapeseed peptides and cottonseed peptides promote the proliferation of lactobacillus acidophilus and bifidobacterium longum.
Recovery and activation of lactobacillus acidophilus
And (3) resuscitation: taking lactobacillus acidophilus freeze-dried powder, dissolving the powder by using sterile MRS liquid to prepare a culture medium, inoculating the dissolved bacteria liquid into an MRS solid culture medium, carrying out anaerobic culture at 37 ℃ for 48h, and observing the growth condition of lactobacillus acidophilus at intervals in the culture process to finish the recovery process.
And (3) activation: activating after the lactobacillus acidophilus is revived, inoculating 1ml of bacterial liquid into 10ml of culture medium each time in the activation process for subculture activation, keeping the culture condition unchanged, and continuously carrying out three times of subculture and then preserving the bacterial liquid for later use.
Secondly, rapeseed peptide and cottonseed peptide promote growth of lactobacillus acidophilus
Respectively preparing 5ml of MRS liquid culture medium (the concentration is 0.1mg/ml, 1mg/ml, 5mg/ml, 8mg/ml and 10mg/ml) containing rapeseed peptides and cottonseed peptides with different concentrations, respectively selecting lactobacillus acidophilus single colonies with uniform sizes, respectively placing the lactobacillus acidophilus single colonies in the culture medium, carrying out anaerobic culture at 37 ℃, and measuring the absorbance value of a bacterial liquid at 600nm wavelength by using an ultraviolet spectrophotometer after 48 hours to obtain the growth influence of the rapeseed peptides and the cottonseed peptides with different concentrations on the lactobacillus acidophilus.
Thirdly, rapeseed peptide and cottonseed peptide promote the growth of bifidobacterium longum
The steps are the same as above.
And (3) test results:
as shown in tables 1 to 4 and FIGS. 1 to 4, the optimum concentrations of the rapeseed peptides and the cottonseed peptides for promoting the growth of Lactobacillus acidophilus and Bifidobacterium longum were 10mg/ml, and it can be seen from the figures that the polypeptide concentrations of 5mg/ml or more had significant effects on promoting the proliferation of probiotics.
TABLE 1 optimal concentration of rapeseed peptides for promoting growth of Lactobacillus acidophilus
Figure BDA0003172682160000102
TABLE 2 optimal concentration of cottonseed peptide for promoting growth of lactobacillus acidophilus
Figure BDA0003172682160000111
TABLE 3 rapeseed peptides optimum concentration for promoting Bifidobacterium longum growth
Figure BDA0003172682160000112
TABLE 4 optimal concentration of cottonseed peptide for promoting growth of Bifidobacterium longum
Figure BDA0003172682160000113
The foregoing is only a preferred embodiment of the present invention, and it should be noted that, for those skilled in the art, various modifications and decorations can be made without departing from the principle of the present invention, and these modifications and decorations should also be regarded as the protection scope of the present invention.

Claims (10)

1. The peptide-containing probiotic composition is characterized by comprising the following components in parts by weight: 20-30 parts of lactobacillus acidophilus, 20-30 parts of bifidobacterium longum, 21-26 parts of rapeseed peptide and 21-26 parts of cottonseed peptide.
2. The peptide-containing probiotic composition according to claim 1, characterized in that it comprises the following components in parts by weight: 23-27 parts of lactobacillus acidophilus, 23-27 parts of bifidobacterium longum, 23-24 parts of rapeseed peptides and 23-24 parts of cottonseed peptides.
3. The peptide-containing probiotic composition according to claim 1 or 2, characterized in that the preparation method of the rapeseed peptides comprises the following steps:
A1) mixing rapeseed cake meal with water to obtain rapeseed suspension;
A2) mixing the rapeseed suspension with alkaline protease, and carrying out enzymolysis to obtain an enzymolysis solution;
A3) after the pH value of the enzymolysis liquid is adjusted to be neutral, centrifuging, filtering and collecting filtrate to obtain rapeseed peptide;
the preparation method of the cottonseed peptide comprises the following steps:
B1) mixing cottonseed cake meal with water to obtain cottonseed suspension;
B2) mixing the cottonseed suspension with alkaline protease, and carrying out enzymolysis to obtain an enzymolysis solution;
B3) and adjusting the pH value of the enzymolysis solution to be neutral, centrifuging, filtering, and collecting filtrate to obtain the cottonseed peptide.
4. The peptide-containing probiotic composition according to claim 3, characterized in that the mass-to-volume ratio of the rapeseed cake and water in step A1) is 1g (8-12 ml); and in the step B1), the mass-to-volume ratio of the cottonseed cake meal to the water is 1g (8-12 ml).
5. The peptide-containing probiotic composition according to claim 3, wherein the temperature of the enzymolysis in step A2) or B2) is 48-52 ℃, the enzymolysis is accompanied by shaking, the pH value of the enzymolysis solution is adjusted to 8.0 once every 8-12 min in the enzymolysis, and the enzymolysis is finished when the pH value of the enzymolysis solution is maintained at 8.0 and is not changed along with the enzymolysis.
6. The peptide-containing probiotic solid beverage is characterized by comprising the following components in parts by weight: 82-122 parts of a peptide-containing probiotic composition according to any one of claims 1-5, 25-30 parts of a resistant dextrin, 4-8 parts of a maltodextrin and 18-22 parts of fructo-oligosaccharides.
7. The peptide-containing probiotic solid beverage according to claim 6, characterized by comprising the following components in parts by weight: 24-26 parts of lactobacillus acidophilus, 24-26 parts of bifidobacterium longum, 23-24 parts of rapeseed peptide, 23-24 parts of cottonseed peptide, 26-28 parts of resistant dextrin, 5-7 parts of maltodextrin and 19-21 parts of fructo-oligosaccharide.
8. The method for preparing the peptide-containing probiotic solid beverage according to claim 6 or 7, comprising the steps of:
1) mixing rapeseed peptide, cottonseed peptide, resistant dextrin, maltodextrin, fructo-oligosaccharide and water to obtain a mixed material;
2) freeze-drying the mixed material to obtain a semi-finished product of the solid beverage;
3) and mixing the semi-finished solid beverage, lactobacillus acidophilus and bifidobacterium longum to obtain the peptide-containing probiotic solid beverage.
9. The preparation method according to claim 8, wherein the mixing in step 1) is accompanied by stirring, and the stirring time is 20-30 min.
10. The preparation method according to claim 8, wherein the mixing in step 3) is accompanied by stirring, and the stirring time is 30-40 min.
CN202110823392.1A 2021-07-21 2021-07-21 Peptide-containing probiotic composition, peptide-containing probiotic solid beverage and preparation method thereof Pending CN113475663A (en)

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